In-vivo Delivery Research Articles

Particle‐Mediated Gene Delivery In Vivo and In Vitro

Particle-mediated or "gene gun" technology has been developed as a nonviral method for gene transfer into various mammalian tissues. Gene delivery is achieved by physical force: a strong shock wave is generated that accelerates DNA-coated gold particles to high speeds, providing them with the momentum needed to penetrate the targeted cells. This unit describes general procedures for in vivo and in vitro DNA and RNA transfections by particle-mediated delivery. The Basic Protocol and an alternate protocol address in vivo delivery to mouse skin. In vitro delivery to cryopreserved and adherent cells is also described.

Limitations of Cationic Liposomes for Antisense Oligonucleotide Delivery in Vivo

Much attention has focused on cationic liposomes for delivery of antisense oligonucleotides. While these reagents demonstrate efficient delivery and potentiation of antisense oligonucleotides in vitro under serum-free conditions, then-use in vivo may be limited. This report highlights issues including serum-instability, biodistribution, and target-nonspecificity that may hinder their use for efficient nucleic acid delivery in vivo. Given the many advantages of liposomes for drug delivery, alternative liposome formulations of nucleic acid are discussed.

Magnetic microspheres: a model system of site specific drug delivery in vivo.

SummaryA novel carrier system for the delivery of chemotherapeutic agents by magnetic means to desired sites has been developed. Results indicate that the carrier, albumin microspheres with entrapped Fe3O4, and adriamycin HCl, can be concentrated at a predetermined site in vivo by a magnetic field. Carrier delivery of adriamycin is supported by the presence of a significant concentration of the drug at the site of carrier localization. This delivery system allows for the accumulation of local adriamycin which is comparable to that achieved by administration of a 100-fold higher dose of the free drug.