In order to explore the influence of C14 alkane on physiological stress responses, mineral nutrient elements uptake, cadmium (Cd) transfer, and uptake characteristics of Lolium perenne L. (ryegrass), a series of pot trials were conducted which included a moderate level of Cd (2.182mg·kg-1) without (control) and with five levels of C14 alkane (V/m, 0.1%, 0.2%, 0.5%, 1%, 2%). Biomass and Cd content in the root and shoot, chlorophyll content, antioxidant enzymes activity, and mineral nutrient elements in the shoot of ryegrass were determined at the end of the experiment. The results indicated that Cd uptake significantly elevated at 0.1% C14 alkane treatment, then gradually decreased with the increase of C14 alkane concentration. Compared with the control, chlorophyll content was significantly suppressed and malondialdehyde (MDA) concentration obviously increased. Superoxide dismutase (SOD) activity and catalase (CAT) activity significantly increased to prevent the C14 alkane stress. With the increase of C14 alkane, the Mn concentration gradually increased; Mg and Fe significantly decreased. Correlation analysis showed that Mn was positively correlated with SOD (with the exception of 2% treatment) and CAT (p < 0.01), and negatively correlated with Cd uptake (p < 0.01). It implied that the increase of Mn induced by C14 alkane stress was an important reason for the decrease of Cd uptake.