Deep-sea Environments Research Articles

A differential-based method for correcting ultra-short baseline positioning results

The primary factors influencing the positional accuracy of an ultra-short baseline (USBL) system are errors in sound speed, delay, installation deviation, and the bending of sound rays. Premised on the USBL positioning model, an error contribution model including ranging error, delay error, sound speed error and array element installation deviation was constructed. In parallel, an indirect difference correction method with additional coefficients was proposed based on the geometric correlation of the error function model. Specifically, a reference beacon was deployed on the seabed, and differential correction was achieved by directly computing target coordinates, integrating them with the positional data from the reference beacon. The proposed method demonstrated efficacy in mitigating errors associated with gradual variations or pronounced geometric correlations, including discrepancies in sound speed, bending of sound rays, and deviations in the installation of array elements. The correction method was applied to the USBL positioning, and the simulation results show that the proposed method notably enhanced both accuracy and consistency in positioning, particularly in shallow water environments characterised by significant sound speed errors. The sea trial data from the R/V Dayang Yihao in the Western Pacific demonstrate that the proposed method can effectively mitigate the errors induced by sound ray bending in deep-sea environments. The mean absolute error (MAE) and standard deviation (STD) were reduced by 51.3% and 55.3%, respectively, compared with the results of the USBL direct solution.

Phytoplankton-derived polysaccharides and microbial peptidoglycans are key nutrients for deep-sea microbes in the Mariana Trench

BackgroundThe deep sea represents the largest marine ecosystem, driving global-scale biogeochemical cycles. Microorganisms are the most abundant biological entities and play a vital role in the cycling of organic matter in such ecosystems. The primary food source for abyssal biota is the sedimentation of particulate organic polymers. However, our knowledge of the specific biopolymers available to deep-sea microbes remains largely incomplete. One crucial rate-limiting step in organic matter cycling is the depolymerization of particulate organic polymers facilitated by extracellular enzymes (EEs). Therefore, the investigation of active EEs and the microbes responsible for their production is a top priority to better understand the key nutrient sources for deep-sea microbes.ResultsIn this study, we conducted analyses of extracellular enzymatic activities (EEAs), metagenomics, and metatranscriptomics from seawater samples of 50–9305 m from the Mariana Trench. While a diverse array of microbial groups was identified throughout the water column, only a few exhibited high levels of transcriptional activities. Notably, microbial populations actively transcribing EE genes involved in biopolymer processing in the abyssopelagic (4700 m) and hadopelagic zones (9305 m) were primarily associated with the class Actinobacteria. These microbes actively transcribed genes coding for enzymes such as cutinase, laccase, and xyloglucanase which are capable of degrading phytoplankton polysaccharides as well as GH23 peptidoglycan lyases and M23 peptidases which have the capacity to break down peptidoglycan. Consequently, corresponding enzyme activities including glycosidases, esterase, and peptidases can be detected in the deep ocean. Furthermore, cell-specific EEAs increased at 9305 m compared to 4700 m, indicating extracellular enzymes play a more significant role in nutrient cycling in the deeper regions of the Mariana Trench.ConclusionsTranscriptomic analyses have shed light on the predominant microbial population actively participating in organic matter cycling in the deep-sea environment of the Mariana Trench. The categories of active EEs suggest that the complex phytoplankton polysaccharides (e.g., cutin, lignin, and hemicellulose) and microbial peptidoglycans serve as the primary nutrient sources available to deep-sea microbes. The high cell-specific EEA observed in the hadal zone underscores the robust polymer-degrading capacities of hadal microbes even in the face of the challenging conditions they encounter in this extreme environment. These findings provide valuable new insights into the sources of nutrition, the key microbes, and the EEs crucial for biopolymer degradation in the deep seawater of the Mariana Trench.6w3UcpMmGbcStWK5zJqVACVideo

A new chemosymbiotic bivalve species of the genus Acharax Dall, 1908 (Bivalvia, Solemyida, Solemyidae) from the Haima cold seep of the South China Sea.

Solemyidae is an ancient group of protobranch bivalves that typically inhabit unusual environments, such as deep-sea chemosynthetic environments, and are symbiotic with chemoautotrophic and gill-hosted bacteria. In May 2018, a living solemyid bivalve was collected using a remotely operated vehicle at a depth of 1,390 m from the Haima cold seep in the northwestern slope of the South China Sea. Through a comprehensive taxonomic approach combining morphological observations and molecular phylogeny reconstruction of concatenated mitochondrial COI,16S rRNA and 18S rRNA gene sequences, a new species, Acharaxhaimaensissp. nov. is identified and described. The discovery of this new species contributes to the diversity of known solemyids in deep-sea chemosynthetic environments.

Effect of hydrostatic pressure on the anodic dissolution process of X80 steel

Based on the fact that hydrostatic pressure affects chemical potentials of reaction species and the structure evolution of electric double layer (EDL), the authors propose a generalized-BV equation to analyze the anodic dissolution process of X80 steel in deep-sea environment. Combined with the results of potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) tests, the following conclusions can be drawn: hydrostatic pressure can compress the electric double layer non-uniformly. The CHCD (CD: capacitance of diffused layer; CH: capacitance of compact layer) value increases first and then decreases with increasing hydrostatic pressure and reaches its maximum at 15 MPa, which is sure to influence the difference in electrical potential that drives chemical reaction at interface. Ultimately, the anodic dissolution current increases and then decreases with rising hydrostatic pressure at the same anodic polarized potential. Besides, low hydrostatic pressure may contribute to forming a dense corrosion product film and inducing a good inhibiting effect on further dissolution of X80 steel; However, when the hydrostatic pressure exceeds the critical value of 20Mpa, too high anodic dissolution rate along with promoted Cl− adsorption will deteriorate the protection performance of corrosion product film, and exacerbate local corrosion process.

Strong linkage between benthic oxygen uptake and bacterial tetraether lipids in deep-sea trench regions

Oxygen in marine sediments regulates many key biogeochemical processes, playing a crucial role in shaping Earth’s climate and benthic ecosystems. In this context, branched glycerol dialkyl glycerol tetraethers (brGDGTs), essential biomarkers in paleoenvironmental research, exhibit an as-yet-unresolved association with sediment oxygen conditions. Here, we investigated brGDGTs in sediments from three deep-sea regions (4045 to 10,100 m water depth) dominated by three respective trench systems and integrated the results with in situ oxygen microprofile data. Our results demonstrate robust correlations between diffusive oxygen uptake (DOU) obtained from microprofiles and brGDGT methylation and isomerization degrees, indicating their primary production within sediments and their strong linkage with microbial diagenetic activity. We establish a quantitative relationship between the Isomerization and Methylation index of Branched Tetraethers (IMBT) and DOU, suggesting its potential validity across deep-sea environments. Increased brGDGT methylation and isomerization likely enhance the fitness of source organisms in deep-sea habitats. Our study positions brGDGTs as a promising tool for quantifying benthic DOU in deep-sea settings, where DOU is a key metric for assessing sedimentary organic carbon degradation and microbial activity.

Influence of cathodic polarization on stress corrosion cracking susceptibility of 35CrMo steel for high strength bolt in simulated deep-sea environment

The effect of cathodic polarization on stress corrosion cracking (SCC) of 35CrMo steel for high strength bolt in the simulated deep-sea environment has been studied. The results show that 35CrMo steel has a higher SCC sensitivity at open circuit potential in the simulated deep-sea environment than that in the shallow seawater. With potential negatively shifted to −750 mV, 35CrMo steel presents the lowest SCC sensitivity. And the SCC susceptibility is increased significantly with the further negative shift of potential because of the intensive hydrogen evolution. When the potential is below −950 mV, hydrogen embrittlement with hydrogen-enhanced-plasticity mediated decohesion should be the dominant SCC micro-mechanism.

Stress corrosion behavior and mechanism of Ti6321 alloy with different microstructures in stimulated deep-sea environment

The stress corrosion cracking (SCC) behaviors of Ti-6Al-3Nb-2Zr-1Mo (Ti6321, in wt%) alloy samples with equiaxed microstructure (EM), bimodal microstructure (BM) and Widmanstätten microstructure (WM) in simulated 3000 m deep sea environment were investigated using electrochemical tests, electron backscattered diffraction (EBSD), and so on. WM exhibits the best SCC resistance, which is attributed to its best self-healing ability, and is beneficial for the re-passivation of fresh metal at the crack tip. Besides, there is no obvious micro-texture region in WM with higher β phase, and α phase colonies hinder the dislocation movement, resulting in the lowest crack growth rate.

CFD-Based Study on the Flow and Kinetic Energy Characteristics of a Supercritical Suspended Abrasive Water Jet in the Deep-Sea Environment

A supercritical suspended abrasive water jet with dual inputs of pressure and heat is proposed to improve the cutting performance of the conventional suspended abrasive water jet in deep-sea environments. The paper studies the flow and kinetic characteristics of the supercritical suspended abrasive water jet. The CFD simulation method is proposed to investigate these characteristics by integrating a programmed database of supercritical water material properties with Ansys Fluent. The simulation and comparison show that abrasive particle density, abrasive particle size, inlet pressure, and water temperature affect the acceleration process of the abrasive particles. At the nozzle outlet, the velocity of the abrasive particles reaches over 95% of the supercritical water velocity. With the proposed supercritical abrasive water jet, the jet velocity is increased by 192.2% to 402.40 m/s compared to the conventional suspended abrasive water jet, reducing the amount of water used by 67.7% at a specified temperature of 773.15 K. Correspondingly, the medium kinetic energy is increased by 177.7% and the medium kinetic energy ratio is 2.78. The particle kinetic energy is increased by 723.2% and the particle kinetic energy ratio is 8.23.

Exceptionally high levels of total mercury in deep-sea sharks of the Southeastern Mediterranean sea over the last ∼ 40 years

Deep-sea habitats are currently recognized as a hot spot for mercury (Hg) accumulation from anthropogenic sources, resulting in elevated concentrations of total mercury (THg) in deep-sea megafauna. Among them, deep-sea sharks (Class Chondrichthyes) are characterized by high trophic position and extended longevity and are, therefore, at high risk for mercury contamination. Despite this, sharks are overexploited by fishing activity in increasingly deeper water, worldwide, imposing health risks to human consumption. While it is imperative to better understand long-term mercury contamination in deep-sea megafauna, few historical data sets exist to capture this process. Here we explore four decades (1985–2022) of THg accumulation in five species of deep-sea sharks (G. melastomus, E. spinax, S. rostratus, C. granulosus, and D. licha) of the ultra-oligotrophic Southeastern Mediterranean Sea (SEMS) sampled during 19 research cruises. We exhibited exceptionally high THg levels (per length/weight), the highest as 16.6 μg g−1 (wet wt.), almost entirely (98.9 %; n = 298 specimens) exceeding the limit for safe consumption (0.3–0.5 μg THg g−1 wet wt.). The maximal THg levels of the long-lived species D. licha and C. granulosus in the SEMS were enriched by a factor of ∼ 7 and >10 compared to counterpart species from other oceanic areas, respectively. We attribute this to the ultra-oligotrophic conditions of the SEMS, which cause slower growth rates and dwarfism in deep-sea sharks, resulting in an extended exposure time to mercury contamination. In the long-lived species, C. granulosus and D. licha, a temporal increase of average THg levels of ∼ 80 % was recorded between 1987–1999 and 2021–2022. This likely reflects the long-term accumulation of historical anthropogenic Hg in deep-sea environments, which is further amplified in marginal seas such as the Mediterranean, impacted by global air pollution crossroads and surrounded by land-based pollution sources. Future consumption of products from deep-sea sharks is potentially high risk to human health.

Qipengyuania benthica sp. nov. and Qipengyuania profundimaris sp. nov., two novel Erythrobacteraceae members isolated from deep-sea environments.

Two Gram-stain-negative, rod-shaped and non-motile strains, designated as DY56-A-20T and G39T, were isolated from deep-sea sediment of the Pacific Ocean and deep-sea seawater of the Indian Ocean, respectively. Strain DY56-A-20T was found to grow at 15-37 °C (optimum, 28 °C), at pH 6.0-10.0 (optimum, pH 6.5-7.0) and in 0.5-6.0 % (w/v) NaCl (optimum, 1.0-2.0 %), while strain G39T was found to grow at 10-42 °C (optimum, 35-40 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and in 0-12.0 % (w/v) NaCl (optimum, 1.0-2.0 %). The 16S rRNA gene sequence identity analysis indicated that strain DY56-A-20T had the highest sequence identity with Qipengyuania marisflavi KEM-5T (97.6 %), while strain G39T displayed the highest sequence identity with Qipengyuania citrea H150T (98.8 %). The phylogenomic reconstruction indicated that both strains formed independent clades within the genus Qipengyuania. The digital DNA-DNA hybridization and average nucleotide identity values between strains DY56-A-20T/G39T and Qipengyuania/Erythrobacter type strains were 17.8-23.8 % and 70.7-81.1 %, respectively, which are below species delineation thresholds. The genome DNA G+C contents were 65.0 and 63.5 mol% for strains DY56-A-20T and G39T, respectively. The predominant cellular fatty acids (>10 %) of strain DY56-A-20T were C17 : 1 ω6c, summed feature 8 and summed feature 3, and the major cellular fatty acids of strain G39T were C17 : 1 ω6c and summed feature 8. The major polar lipids in both strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and an unidentified polar lipid. The only respiratory quinone present in both strains was ubiquinone-10. Based on those genotypic and phenotypic results, the two strains represent two novel species belonging to the genus Qipengyuania, for which the names Qipengyuania benthica sp. nov. and Qipengyuania profundimaris sp. nov. are proposed. The type strain of Q. benthica is DY56-A-20T (=MCCC M27941T=KCTC 92309T), and the type strain of Q. profundimaris is G39T (=MCCC M30353T=KCTC 8208T).

A new chemosynthetic community (ostracods, foraminifers, echinoderms) from Late Jurassic hydrocarbon seeps, south-eastern France Basin

Hydrocarbon seeps represent some of the most extreme marine habitats but are also home to rich communities developed around chemosynthesis rather than photosynthesis. Here we describe the outcrop of Sahune (Drôme department, south-eastern France), that illustrates a new seeping site during the Late Jurassic (middle Oxfordian), as formally demonstrated by geochemical proxies. We report the associated fauna composed of foraminifers, radiolarians, crinoids, echinoids and ostracods that all point to seepage at bathyal depth. The foraminifer assemblage and the occurrence of the irregular echinoid Tithonia oxfordiana together point to a middle Oxfordian age. We provide an in-depth analysis of the ostracod community, which is the oldest so far reported in such environments. The new species Procytherura praecoquum may be cognate to the seepage site and could illustrate the oldest known example of pore clusters, sometimes proposed as representing ectosymbiosis. The Sahune assemblage demonstrates that cold seep ostracod communities were already a mixture of taxa from platform and deep-sea oligotrophic environments. The post-Jurassic diversification of ostracods at cold seeps was related to colonization events and diversification of families that have been inhabitants of such ecosystems at least since the Oxfordian. The Sahune record changes our current conception of the deep-sea colonization by the ostracods Tethysia and Procytherura that occurred earlier than traditionally considered.

Failure mechanism of Al-Zn-In sacrificial anode under the synergic action of water pressure and fluid in the extreme deep-sea environment

Corrosion failure of sacrificial anodes in extreme deep-sea environment is a key problem in metal protection technology. This study investigated the failure mechanism of the Al-Zn-In sacrificial anode under the synergic action of water pressure and fluid flow, by microstructure characterization and finite element analysis. The results exhibit that water pressure promotes the dissolution of Al-Zn-In alloy near the precipitates that are enriched at the grain boundaries. The flow amplifies the promoting effect of the pressure on anodic dissolution by inhibiting the formation of corrosion layers, and it accelerates the mass loss by mechanically aggressing the aluminum matrix. The synergic effect of water pressure and fluid flow results in a rapid degradation in the extreme deep-sea environment.

Identification and genomic analysis of a novel temperate bacteriophage infecting Labrenzia aggregata isolated from the Mariana Trench

In marine environments, viruses play a pivotal role, yet deep-sea bacteriophages remains largely uncharacterized. The bacterium Labrenzia aggregata RF14, isolated from the Mariana Trench at a depth of 4,000 meters, harbors prophage regions based on a previous study. In this study, we induced a temperate bacteriophage from it using mitomycin C. The bacteriophage exhibited an icosahedral structure with a non-extendable tail and was named vB_LagS-V1. The genome size of it is 39,329 bps with a 59.46% G+C content, encoding 60 putative open reading frames. Genomic and phylogenetic analyses demonstrated that vB_LagS-V1 along with many bacteriophages infecting Hyphomicrobiales, constituted a newly unclassified family, which we designated as Hyphoviridae. Within this novel family, vB_LagS-V1 is distinct with isolated phages and clustered with two uncultured prophages within Labrenzia, forming an unclassified new genus, given a name of Labrenmarinevirus. The codon usage correlation and absence of tRNAs found in vB_LagS-V1, also prevail in some deep-sea bacteriophages, highlighting their adaptations to the deep-sea prokaryotic hosts. Moreover, vB_LagS-V1 encoded two auxiliary metabolic genes, cysteine dioxygenase and phosphoadenosine phosphosulfate reductase, which might help the phage and its host adapt to high hydrostatic pressure in the deep-sea environments. Our study will significantly contribute to the understanding of deep-sea bacteriophages and their interactions with hosts in extreme environments.

Geochemical proxies for deep-sea temperature and nutrient content in cold-water bamboo corals

The impact of warming, acidification, and deoxygenation on deep-sea environments is a growing concern. Historical records are sparse, particularly at high latitudes, making climate change projections challenging. Indirect proxies, such as trace element composition of marine carbonates, such as coral skeletons, can offer an alternative method to fill data gaps but have not been realised. Here, using Laser Ablation Triple-Quadrupole Inductively Coupled Plasma Mass Spectrometry (LA-QQQ-ICP-MS), we examined micrometre-scale element variation within and between individual colonies of the bamboo coral Keratoisis sp. obtained from the Eastern Canadian Arctic. These data are used to assess the influence of biological variability on geochemical tracers for reconstructing past environmental conditions (temperature: Mg/Ca, Li/Mg, Sr/Ca, Ba/Ca, U/Ca; [Ba]SW: Ba/Ca). We place these data into context, based on a survey of literature data, using refined calibrations for high-Mg calcitic Octocorals. We find reproducible (2σ relative coefficient of variation) values of Mg/Ca (3%) and Ba/Ca (6%) along the radial growth axis of all colonies and internodes of Keratoisis sp., indicating that these signals are likely suitable for environmental reconstructions. After revising the available multi-taxa calibrations for Mg/Ca (0.316 ± 0.026 °C/mmol/mol, R2 = 0.87, p < 0.001) and Ba/Ca ([Ba/Ca μmol/mol] = 0.148 ± 0.005 [BaSW nmol/kg], R2 = 0.97, p < 0.001), we show that vital effects within and among Keratoisis sp. colonies strongly influence reconstructed temperature and [Ba]SW, but this can be somewhat mitigated by combining multiple internode transects from one colony into a single composite series. Despite the ontogenetic variability, all colonies reveal a gradual deep-water cooling trend since the early 21st century and synchronised, multi-year spikes in Ba/Ca (and hence [Ba]SW) that suggest substantial and coherent barium inputs to the seafloor. Our study confirms the reliability of Mg/Ca and Ba/Ca proxies in high-Mg bamboo corals for detecting multi-annual temperature and seawater barium variations in cold-water environments, but further investigation into micro-scale element behaviour influenced by biotic processes in these corals is needed to enhance confidence in reconstructions at finer spatial and temporal resolutions. We conclude that employing empirical calibrations based on multi-taxa approaches can increase the certainty of capturing regional changes in the environment more accurately than a single species calibration, while leveraging multiple element series to account for biological-induced variability improves single colony reconstructions.

A Highly Sensitive Deep-Sea Hydrodynamic Pressure Sensor Inspired by Fish Lateral Line.

Hydrodynamic pressure sensors offer an auxiliary approach for ocean exploration by unmanned underwater vehicles (UUVs). However, existing hydrodynamic pressure sensors often lack the ability to monitor subtle hydrodynamic stimuli in deep-sea environments. In this study, we present the development of a deep-sea hydrodynamic pressure sensor (DSHPS) capable of operating over a wide range of water depths while maintaining exceptional hydrodynamic sensing performance. The DSHPS device was systematically optimized by considering factors such as piezoelectric polyvinylidene fluoride-trifluoroethylene/barium titanate [P(VDF-TrFE)/BTO] nanofibers, electrode configurations, sensing element dimensions, integrated circuits, and packaging strategies. The optimized DSHPS exhibited a remarkable pressure gradient response, achieving a minimum pressure difference detection capability of approximately 0.11 Pa. Additionally, the DSHPS demonstrated outstanding performance in the spatial positioning of dipole sources, which was elucidated through theoretical charge modeling and fluid-structure interaction (FSI) simulations. Furthermore, the integration of a high Young's modulus packaging strategy inspired by fish skull morphology ensured reliable sensing capabilities of the DSHPS even at depths of 1000 m in the deep sea. The DSHPS also exhibited consistent and reproducible positioning performance for subtle hydrodynamic stimulus sources across this wide range of water depths. We envision that the development of the DSHPS not only enhances our understanding of the evolutionary aspects of deep-sea canal lateral lines but also paves the way for the advancement of artificial hydrodynamic pressure sensors.

Mitochondrial and morphological adaptions of Lindaspio polybranchiata (Annelida: Spionidae) in the South China Sea

A dense population of Lindaspio polybranchiata (Annelida: Spionidae) was discovered in a novel cold seep with drastic methane seepage located on the northern passive continental margin of the South China Sea. L. polybranchiata may function as a pioneer species by rapidly colonizing this nascent methane seep. We studied its classification status and evolutionary selection adaptation based on the mitochondrial genome. Phylogenetic analysis revealed that the deep-sea genus Lindaspio clustered with the neritic Rhynchospio aff. asiatica, and the ancestor of the deep-sea Lindaspio might originate from shallow-water spionids. Twelve potentially adaptive mutations were identified in cox1, cox3, cytb, and nad5. The morphological characteristics of L. polybranchiata and R. aff. asiatica collected in the intertidal zones were systematically compared to explore the possible phenotypic adaptations of L. polybranchiata to extreme deep-sea environments. L. polybranchiata lost its eyespots due to long-term colonization of the dark habitat in the deep sea but developed an enlarged caruncle for facilitating chemical perception in the darkness. The specific ventral branchiae and extended respiratory surfaces may aid the survival of L. polybranchiata in the hypoxic environment of the cold seep. This study provides novel insights into the molecular and morphological adaptations of a species endemic in chemosynthetic ecosystems.

Deep sea treasures - Insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems

Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phylum. The technical limitations and the high cost of deep sea surveys have made exploring deep sea environments and the biology of the organisms that inhabit them challenging. In spite of the widespread recognition of Cnidaria's environmental importance in these ecosystems, the microbial assemblage and its role in coral functioning have only been studied for a few deep water corals. Here, we explored the microbial diversity of deep sea corals by recovering nucleic acids from museum archive specimens. Firstly, we amplified and sequenced the V1–V3 regions of the 16S rRNA gene of these specimens, then we utilized the generated sequences to shed light on the microbial diversity associated with seven families of corals collected from depth in the Coral Sea (depth range 1309 to 2959 m) and Southern Ocean (depth range 1401 to 2071 m) benthic habitats. Surprisingly, Cyanobacteria sequences were consistently associated with six out of seven coral families from both sampling locations, suggesting that these bacteria are potentially ubiquitous members of the microbiome within these cold and deep sea water corals. Additionally, we show that Cnidaria might benefit from symbiotic associations with a range of chemosynthetic bacteria including nitrite, carbon monoxide and sulfur oxidizers. Consistent with previous studies, we show that sequences associated with the bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes and Acidobacteriota dominated the microbial community of corals in the deep sea. We also explored genomes of the bacterial genus Mycoplasma, which we identified as associated with specimens of three deep sea coral families, finding evidence that these bacteria may aid the host immune system. Importantly our results show that museum specimens retain components of host microbiome that can provide new insights into the diversity of deep sea coral microbiomes (and potentially other organisms), as well as the diversity of microbes writ large in deep sea ecosystems.

Structure design and deformation analysis of double hydraulic cylinder deep-sea pressure simulator

Because of the particularity of the deep-sea environment and the difficulty of on-site observation and research, it is particularly important to establish a deep-sea simulation system for research. Based on the high measurement accuracy requirement of deep-sea simulation equipment, a design scheme of a deep-sea pressure simulation device with a double hydraulic cylinder that can measure small water pressure changes is proposed. Firstly, the working parameters and pressure principle of the design are introduced, and then the deformation of the deep-sea pressure simulation device is analyzed. The mathematical model of piston feed and pressure without considering the deformation of the sealed pressure cylinder and the mathematical model of piston feed and pressure considering the deformation of the sealed pressure cylinder are established respectively. Finally, the relationship curve is analyzed by using origin software. The results show that when the deep-sea pressure simulator is used to simulate the underwater environment, it is necessary to consider not only the influence of liquid compression on the pressure but also the influence of deformation on the pressure.

Quantitative detection algorithm for deep-sea megabenthic organisms based on improved YOLOv5

Detecting deep-sea megabenthic organisms is of foremost importance for seabed resource surveys, typical habitat protection, and biodiversity surveys. However, the complexity of the deep-sea environment, uneven illumination, and small biological targets that are easily obscured all increase target detection difficulty significantly. To address these, this paper proposes a deep-sea megabenthic detection algorithm, DS-YOLO, based on YOLOv5s. To improve the detection ability of the model for deep-sea megabenthic organisms, the space-to-depth module and the spatial pyramid pooling cross stage partial channel module are introduced in the Backbone layer to enlarge the receptive field and enhance the retention of small-scale features. Then, the space-to-depth and normalization-based attention modules and the Add and Concat functions of the bidirectional feature pyramid network are introduced in the Neck layer to increase the multiscale fusion ability of the model and highlight the insignificant features. Finally, the two branches of the decoupling header output the category and location of the target, which causes the model to utilize the feature information to the maximum extent. Experiments showed that DS-YOLO improved mAP0.5 from 89.6% to 92.4% and mAP0.5:0.95 from 65.7% to 72.3% compared to the original YOLOv5s on the homemade dataset and outperformed other algorithms in the YOLO series. DS-YOLO reaches 84.7 FPS for deployment on mobile platforms. In addition, the combined DS-YOLO and DeepSORT algorithm can be used to calculate the abundance and community structure of deep-sea megabenthos. The model outperforms general target detection models for deep-sea megabenthos detection and is suitable for use in complex deep-sea environments.

Microscopic investigation on pitting corrosion of Ni-Cr-Mo-V high-strength steel weldment in simulated deep-sea environment

The pitting corrosion behavior and mechanism of Ni-Cr-Mo-V high-strength low-alloy steel weldment in simulated deep-sea environment were investigated by in-situ electrochemical measurements and microscopic observation techniques. The results show that pitting corrosion is the main degradation form of Ni-Cr-Mo-V steel in the deep sea, and its weldment exhibits more severe localized attack under the macro-galvanic effect at the welded joint. HAZ is more sensitive to pitting due to the inhomogeneity of microstructure and composition. Most pitting is associated with non-metallic inclusions. The Al-enriched inclusions makes greater contribution to pitting than Si-enriched and Ti-enriched inclusions. The hydrostatic pressure in the deep sea promotes pitting, which is induced either by the preferential dissolution of the soluble components of the inclusions or by the micro-crevices and internal stresses formed at inclusion/matrix interface.