Date Palm Fruit Research Articles

Life-table parameters of Plodia interpunctella (Lepidoptera: Pyralidae) on different stored date palm fruits under laboratory conditions.

Indian meal moth, Plodia interpunctella (Hübner) (Lepidoptera: Pyralidae), is a polyphagous insect pest that causes serious damage to various food crops in storage. This study aimed to investigate the life-history and demographic parameters of P. interpunctella on 5 varieties of date palm fruits (Phoenix dactylifera L.), including Dayri, Estemaran, Fersi, Halavi, and Zahedi under laboratory conditions. Data were analyzed and compared using the age-stage, 2-sex life table. Plodia interpunctella completed its development on all date varieties. The shortest and longest pre-adult periods were recorded on Zahedi (38.47 days) and Estemaran (44.65 days) varieties, respectively. The net reproductive rates (R0) were 82.51, 59.05, 63.61, 102.27, and 114.86 offspring on Dayri, Estemaran, Fersi, Halavi, and Zahedi varieties, respectively. The intrinsic rate of increase (r) were 0.098, 0.085, 0.089, 0.109, and 0.113 day-1 on Dayri, Estemaran, Fersi, Halavi, and Zahedi varieties, respectively. The female fecundity ranged from 133.4 to 259.24 eggs on Estemaran and Zahedi varieties, respectively. The highest mean generation time (T) was obtained on Estemaran (47.984 days), and the lowest value of this parameter was obtained on Zahedi (41.722 days) variety. The results indicated that Zahedi and Halavi varieties were the susceptible hosts for P. interpunctella. In contrast, the Estemaran and Fersi were the most resistant varieties against P. interpunctella, which can be used for integrated management programs to decrease the damage of this pest.

Date Palm Fruit (Phoenix dactylifera) and Its Promising Potential in Developing Functional Energy Bars: Review of Chemical, Nutritional, Functional, and Sensory Attributes

Snack bars, known as energy bars, are widely consumed worldwide as highly nutritive on-the-go products. Due to the date fruit’s significant nutritional and functional characteristics, it can be an exceptional choice for developing snack bars. Dates contain a wide range of macro- and micronutrients known for their strong bioactive properties. The functional properties of date fruit have been demonstrated in the literature and include antioxidant, anti-inflammatory, anti-tumor, antihypertensive, and antimicrobial activities. This review summarizes the available studies investigating the potential application of dates for developing nutritive and functional snack bars. Date paste was used as a main ingredient at 55–90% concentrations. In addition, protein sources were used to provide protein-rich snack bars, as date fruit is considered high in carbohydrates and low in protein. Skim milk powder was the most common and favorable protein source, delivering significant amounts of protein with limited negative effects on sensory attributes. Incorporating other ingredients, such as cereals or legumes, was also explored; adding such dry ingredients can promote positive nutritional effects along with improving sensory attributes, mainly in terms of the bars’ textures. Dry ingredients can significantly lower moisture content, reducing the bars’ fracturability to acceptable ranges. Reduced moisture content can also significantly enhance the shelf-life stability, as observed by limited microbial growth. Furthermore, the incorporation of bioactive or functional ingredients such as fruit peels, plant seeds, or plant leaf extracts was also reported; such ingredients promoted significant enhancements in the contents of phenolics or flavonoids, for instance, leading to an increase in the bars’ antioxidant potential. Though dates are rich in such bioactive components, incorporating additional bioactive ingredients can boost the dates’ functional properties. In conclusion, this review shows the high potential of the application of dates for developing nutritious and functional snack bars. Taking this into account, the snack bar market has grown remarkably over the past decade; thus, providing well-balanced, nutritious, and functional date-based bars in markets worldwide is expected to show positive consumer acceptance.

Development and Quality Evaluation of Extruded Breakfast Cereal from Blends of Ofada Rice and Cowpea (Vigna unguiculata) with Date Palm Fruit

Development of food product is a global initiative focused on crops utilization for economic empowerment and service provider. Extruded breakfast cereal developed from unconventional grain, ofada rice blended with cowpea and date palm fruit as natural sweetener was designed and evaluated. Composite flour formulation was in ratio 55:45, 60:40, 65:35, 70:30 while date palm fruit was kept constant. Proximate, functional, pasting, shelf-life and sensory analyses were carried out. Protein and ash content in experimented samples, B1KK, B2KL, B3KM and B4KN increased significantly with increased proportion of cowpea. At 5% level of significance, there was significant difference in water absorption index in all experimented sample while bulk density and swelling power showed no significant difference in most samples. Final viscosity of the samples increased as the proportion of ofada rice decreased while setback viscosity increased as the proportion of cowpea increased. Moisture content, free fatty acid and peroxide value of the samples increased slightly as approaches six weeks storage, however, the result was within internationally acceptable quality limits. Sensory attributes of the designed products are significantly related to the control sample, apparently, consumer perception of the breakfast cereal based products indicated that B2KL was most preferred and thus recommended for commercialization. How to Cite: Musa Omotayo Jimoh, Taiwo Olufemi Olurin, Oyesiku Seun Odunayo, 2023. "Development and Quality Evaluation of Extruded Breakfast Cereal from Blends of Ofada Rice and Cowpea (Vigna unguiculata) with Date Palm Fruit." Journal of Agriculture and Crops, vol. 9, pp. 293-301.

Antioxidant and antibacterial activities of date palm fruit (Phoenix dactylifera L.) in response to postharvest application with natural elicitors

Antioxidant and antibacterial activities of date palm fruit (<i>Phoenix dactylifera</i> L.) in response to postharvest application with natural elicitors

Algerian date palm (Phoenix dactylifera L.) fruit cultivars: HPLC fingerprinting and antibacterial activity

The abusive use of antibiotics causes the destruction of intestinal flora and the proliferation of antibiotic-resistant pathogens. Date palm is used in traditional medicine in the Saharan regions due to its biological properties.&#x0D; The study aimed to identify the phytochemical composition and assess the antibacterial activity of the methanolic extracts of three date cultivars from Algeria. Their total phenolic, flavonoid, and flavonol contents were measured spectrophotometrically. The phytochemical screening was conducted by HPLC fingerprinting using twenty-three pure phenolic compounds as standards. The antibacterial activity against pathogenic bacterial species was assessed using the disk diffusion method. &#x0D; The colorimetric methods showed that the total phenolic, flavonoid, and flavonol contents ranged from 2.13 ± 0.09 to 2.67 ± 0.02 mg GAE/100 g DW, 1.33 ± 0.21 to 1.55 ± 0.13 mg CEQ/100 g DW, and 0.41 ± 0.23 to 0.47 ± 0.05 mg REQ/100 g DW, respectively. HPLC fingerprinting showed that the extracts of date cultivars served as an excellent source of bioactive compounds (gallic acid, tannic acid, ferulic acid, vanillin, caffeine, quercetin, luteolin, rutin, aspegenin, isorhamnetin, and hesperidin). They also exhibited an antibacterial potential with an inhibition zone diameter ranging from 8.40 to 12.50 mm.&#x0D; The results clearly demonstrate the antibacterial potency of date palm fruits, which could be attributed to their considerable content of phenolic compounds such as gallic acid, rutin, quercetin, and luteolin.

Optimization and Intensification of Bioactive Components and Antioxidant Activity of Extracts from Date Fruit (Phoenix dactylifera L.) Using Pulsed Electric Fields (PEF) Technology and Thermal Processing

The objective of this study was to assess the impact of pulsed electric field (PEF) treatment on the extraction of polyphenolics and antioxidant activity from downgraded date palm fruits. The PEF pretreatments (frequency: 30 Hz, time: 50 μs, pulse number: 240, the electric field strengths were found to be 1, 2, and 2.5 kV.cm−1, and methanol (50%) and temperatures (20, 40, 50 °C)) were optimized and applied before extraction to produce an extract of date fruits with a high content of bioactive compounds. The extracts obtained immediately after pretreatments were analyzed for total polyphenolic content, antioxidant activity, and phenolic profiles. The results revealed that the PEF-assisted extracts at 2.5 kV.cm−1 at T50 °C exhibited higher polyphenol content (+27%) and antioxidant activity (+31%) and notably improved phenolic profiles compared to untreated extracts. PEF treatment processing significantly enhanced the bioactive components and antioxidant activities of date fruits over time, regardless of the treatment applied and the extraction’s temperature. Hence, the application of PEF combined with thermal processing can be an appropriate alternative treatment for a better extractability of bioactive compounds from fruit of dates and food byproducts. These biomolecules could be consumed as new food technology, incorporated as food additives, and nutraceuticals products.

The Hepatoprotective Effect of Two Date Palm Fruit Cultivars’ Extracts: Green Optimization of the Extraction Process

Date palm fruit (Phoenix dactylifera: Arecaceae) is rich in essential nutrients and possesses several pharmacological and medicinal activities. The current study aimed to optimize a water bath-assisted extraction method for two cultivars of date palm fruits, Anbara (An) and Reziz (Rz), and investigated the protective effect of the optimized date palm fruit extract against CCl4-induced liver toxicity in relation to oxidative stress, inflammation, apoptosis, and DNA integrity. The optimization process of two date palm fruit cultivars was applied, using response surface methodology through adjusting three "factors"; time, temperature, and rotation, to allow maximum contents of total phenolic (TPC), total flavonoid (TFC), reducing power (FRAP) and scavenging activity (ABTS) of the extract "responses". Extraction factors' application significantly enhanced TPC, TFC, FRAP, and ABTS responses by 1.30, 1.23, 3.03, and 2.06-fold, respectively in An and 2.18, 1.71, 1.11, and 2.62-fold, respectively in Rz, in relation to the convectional water extraction. Furthermore, co-administered CCl4 with An or Rz optimized extracts enhanced body weight gain, amended hepatic architecture, and diminished collagen fiber accumulation. Furthermore, An or Rz extracts reduced liver enzymes, hydroxyproline, alpha-fetoprotein (AFP), MDA, inflammatory cytokine (TNF-α, NF-κB) levels, and DNA fragmentation, while increasing deteriorated adiponectin (ADP) and antioxidant enzyme (GSH, GPX, NO, and IFN-γ) levels, relative to CCl4-administered animals. The protective effects of An or Rz-optimized extracts were also evidenced by suppressing hepatic fibrosis and improving liver function and structure via modulating oxidative stress, inflammation, and apoptosis, in CCl4-induced hepatic damage. Hence, the optimized extraction process for the two date palm fruits resulted in extracts which are rich in phenolic and flavonoid contents and with an elevated antioxidant power. The presence of these rich extracts could help to explain their proven hepatoprotective activity against CCl4-induced liver toxicity.

Enhancing yield and fruit quality of date palm (Phoenix dactyllifera L., cv. Zaghloul) using nitrogen and potassium nano-fertilizer

The present investigation was carried out during two successive seasons of 2018 / 2019 and 2019/ 2020 on 15 years old date palm trees of Zaghloul cv. grown in sandy soil in a private orchard at El-Behira governorate, Egypt. The research was aimed to investigate the effect of nitrogen and potassium nano-fertilizers comparing with conventional mineral fertilizers on yield and fruit quality, four doses of nitrogen and potassium nano-fertilizer (75, 150, 300 and 600g/ palm/ year) were applied compared to the conventional nitrogen and potassium mineral fertilizers as a control. Different fertilization treatments significantly affected palm yield and fruit quality during both seasons. Treatment of 600g of nano N and K fertilizers produced higher values of tree yield and bunch weight, fruit volume, and fruit length in both seasons. Meanwhile, 600g of nano-fertilizer and control treatments gave the highest fruit and flesh weight. The obtained results also showed fruit content of total soluble solids, total and reducing sugars was significantly increased by nano-fertilizer treatment at 600 and 300g per palm and improved significantly N, P, and K content. Whereas treatment with 75 and 150g nano-fertilizer gave higher values of total tannins and total phenols contents in Zaghloul fruits in both seasons. According to the current study, nano-fertilizer improved date palm yield, fruit quality and nutritional status.

The anti-toxic effect of the date palm fruit extract loaded on chitosan nanoparticles against CCl4-induced liver fibrosis in a mouse model

The liver is the most important organ in the body. Hepatocyte oxidative damage occurs to excess ROS. Liver fibrosis is a mechanism that the immune system uses to treat extreme inflammation by repairing damaged tissue with the creation of a scar. The outcome of fibrosis may be reversed by consuming natural plant extracts with high ROS-scavenging ability. The date palm fruits contain caffeic acid, gallic acid, syringic acid, and ferulic acid, which have anti-inflammatory, antioxidant, and hepatoprotective properties. This study aimed to prepare a date fruit extract, load it onto chitosan nanoparticles, and compare its anti-fibrotic activity with the unloaded crude extract in the CCl4-mouse model. Our findings show that nanocomposite (Cs@FA/DEx) has anti-fibrotic properties and can improve liver function enzymes and endogenous antioxidant enzymes by inhibiting cell apoptosis caused by CCl4-induction in mice. Furthermore, significantly reduced CD95 and ICAM1 levels and down-regulation of TGFβ-1 and collagen-α-1 expression demonstrated the anti-fibrotic effects of the Cs@FA/DEx. Therefore, the Cs@FA/DEx might be an innovative supplement for inhibiting liver fibrosis and hepatocyte inflammation induced by chemical toxins. Besides, this nano-supplement could be a promising anti-hepatocellular carcinoma agent as it has potent in vitro anticancer activity against the HePG2 cell line.

Combined Impact of Irrigation, Potassium Fertilizer, and Thinning Treatments on Yield, Skin Separation, and Physicochemical Properties of Date Palm Fruits.

Orchard cultural practices, i.e., irrigation, fertilizer, and fruit thinning, are crucially encompassed to enhance fruit yield and quality. Appropriate irrigation and fertilizer inputs improve plant growth and fruit quality, but their overuse leads to the degradation of the ecosystem and water quality, and other biological concerns. Potassium fertilizer improves fruit sugar and flavor and accelerates fruit ripening. Bunch thinning also significantly reduces the crop burden and improves the physicochemical characteristics of the fruit. Therefore, the present study aims to appraise the combined impact of irrigation, sulfate of potash (SOP) fertilizer, and fruit bunch thinning practices on fruit yield and quality of date palm cv. Sukary under the agro-climatic condition of the Al-Qassim (Buraydah) region, Kingdom of Saudi Arabia. To achieve these objectives, four irrigation levels (80, 100, 120, and 140% of crop evapotranspiration (ETc), three SOP fertilizer doses (2.5, 5, and 7.5 kg palm-1), and three fruit bunch thinning levels (8, 10, and 12 bunches palm-1) were applied. The effects of these factors were determined on fruit bunch traits, physicochemical fruit characteristics, fruit texture profile, fruit color parameters, fruit skin separation disorder, fruit grading, and yield attributes. The findings of the present study showed that the lowest (80% ETc) and highest (140% ETc) irrigation water levels, lowest SOP fertilizer dose (2.5 kg palm-1), and retaining the highest number of fruit bunch per tree (12 bunches) had a negative effect on most yield and quality attributes of date palm cv. Sukary. However, maintaining the date palm water requirement at 100 and 120% ETc, applying SOP fertilizer doses at 5 and 7.5 kg palm-1, and retaining 8-10 fruit bunches per palm had significantly positive effects on the fruit yield and quality characteristics. Therefore, it is concluded that applying 100% ETc irrigation water combined with a 5 kg palm-1 SOP fertilizer dose and maintaining 8-10 fruit bunches per palm is more equitable than other treatment combinations.

Abscisic acid plays a key role in the regulation of date palm fruit ripening.

The date palm (Phoenix dactylifera L.) fruit is of major importance for the nutrition of broad populations in the world's desert strip; yet it is sorely understudied. Understanding the mechanism regulating date fruit development and ripening is essential to customise date crop to the climatic change, which elaborates yield losses due to often too early occurring wet season. This study aimed to uncover the mechanism regulating date fruit ripening. To that end, we followed the natural process of date fruit development and the effects of exogenous hormone application on fruit ripening in the elite cultivar 'Medjool'. The results of the current study indicate that the onset of fruit ripening occurre once the seed had reached maximum dry weight. From this point, fruit pericarp endogenous abscisic acid (ABA) levels consistently increased until fruit harvest. The final stage in fruit ripening, the yellow-to-brown transition, was preceded by an arrest of xylem-mediated water transport into the fruit. Exogenous ABA application enhanced fruit ripening when applied just before the green-to-yellow fruit color transition. Repeated ABA applications hastened various fruit ripening processes, resulting in earlier fruit harvest. The data presented supports a pivotal role for ABA in the regulation of date fruit ripening.

Possible Mechanisms of the Neuroprotective Actions of Date Palm Fruits Aqueous Extracts against Valproic Acid-Induced Autism in Rats.

The current study aimed to determine how palm date aqueous fruit extracts (AFE) affected the autistic-like behaviors brought on by valproic acid (VPA) injection, as well as any potential contributions from Sirt-1, oxidative stress, apoptosis, and autophagy. The pregnant Sprague Dawley females were treated with VPA at 12.5th gestation day and pregnant females and their offspring were treated with AFE orally at doses of 4 mg/Kg by gastric gavage for 45 days after birth. The elevated plus-T maze, water maze, and rotarod tests were used to examine autism-like behaviors. At the end of the study, the expression of Nrf2, heme oxygenase (HO-1), Sirt-1, caspase-3 (a marker of apoptosis), LC3 (a marker of autophagy), and NFκB (inflammatory cytokines) were evaluated along with the oxidative stress in brain tissues and the histological changes in the cerebellum and hippocampus. The neurobehavioral assessments significantly declined due to VPA, which also significantly increased oxidative stress in the brain tissues and significantly decreased Nrf2 and HO-1 expression. Additionally, VPA administration caused significant increase in the expression of caspase-3 in the cerebellar cortex, not in the hippocampus; LC3 and NFκB in the hippocampus, not in the cerebellar cortex; and significant reduction in the expression of Sirt-1 in the hippocampus, not in the cerebellum. On the other hand, AFE treatment significantly improved the neurobehavioral changes as well as it improved significantly the oxidative stress and the expression of LC3, NFκB, NrF2, HO-1, and Sirt-1 in the cerebellum and hippocampus. Conclusions: AFE administration might improve the autistic-like symptoms induced by VPA in rats via attenuation of the oxidative stress, upregulation of Nrf2 and HO-1, Sirt-1 and LC3 expression with downregulation of caspase-3, and NFκB expression in the cerebellum and hippocampus.

Evaluating the effect of pre-storage application with 1-methyle cyclopropene and salicylic acid for enhancing antioxidant enzyme activity and quality of Date palm fruits during cold storage.

Evaluating the effect of pre-storage application with 1-methyle cyclopropene and salicylic acid for enhancing antioxidant enzyme activity and quality of Date palm fruits during cold storage.

Development and Validation of Innovative Machine Learning Models for Predicting Date Palm Mite Infestation on Fruits

The date palm mite (DPM), Oligonychus afrasiaticus (McGregor), is a key pest of unripe date fruits. The detection of this mite depends largely on the visual observations of the webs it produces on the green fruits. One of the most important problems of DPM control is the lack of an accurate decision-making approach for monitoring and predicting infestation on date fruits. Therefore, this study aimed to develop, evaluate, and validate prediction models for DPM infestation on fruits based on meteorological variables (temperature, relative humidity, wind speed, and solar radiation) and the physicochemical properties of date fruits (weight, firmness, moisture content, total soluble solids, total sugar, and tannin content) using two machine learning (ML) algorithms, i.e., linear regression (LR) and decision forest regression (DFR). The meteorological variables data in the study area were acquired using an IoT-based weather station. The physicochemical properties of two popular date palm cultivars, i.e., Khalas and Barhee, were analyzed at different fruit development stages. The development and performance of the LR and DFR prediction models were implemented using Microsoft Azure ML. The evaluation of the developed models indicated that the DFR was more accurate than the LR model in predicting the DPM based on the input variables, i.e., meteorological variables (R2 = 0.842), physicochemical properties variables (R2 = 0.895), and the combination of both meteorological and the physicochemical properties variables (R2 = 0.921). Accordingly, the developed DFR model was deployed as a fully functional prediction web service into the Azure cloud platform and the Excel add-ins. The validation of the deployed DFR model showed that it was able to predict the DPM count on date palm fruits based on the combination of meteorological and physicochemical properties variables (R2 = 0.918). The deployed DFR model by the web service of Azure Ml studio enhanced the prediction of the DPM count on the date fruits as a fast and easy-to-use approach. These findings demonstrated that the DFR model using Azure Ml Studio integrated into the Azure platform can be a powerful tool in integrated DPM management.

Bioethanol Production from Waste and Nonsalable Date Palm (Phoenix dactylifera L.) Fruits: Potentials and Challenges

Balancing the needs of current and future generations stimulates investment for sustainable development such as converting waste biomass into biorenewables. Sugar-based ethanol production is a well-investigated and established process, and researchers are now focusing on the transformation of cellulosic biomass to sugar and the application of non-conventional methods for ethanol production. The State of Qatar generates date palm fruit waste of approximately 4505 tons annually, excluding ornamental palms and palms outside the farms that bear nonmarketable date fruits. Date fruit molasses contains fermentable sugars, representing 75% of the total fruit mass, which can offer a good source for bioethanol production through anaerobic fermentation and distillation. On this basis, the valorization of waste date fruits can be an effective zero-waste strategy via biotransformation into bio-renewable materials, hence, contributing to the achievement of sustainable development goals. This paper reviews the potentials and challenges for the utilization of waste date fruits as a bioethanol source and assesses the abundance of waste date fruits as raw material for the conventional bioethanol conversion process. The review also identified conventional and nonconventional processes for bioethanol production and their applicability to Qatar. Finally, this confirmed ample demand for bioethanol ranging from fuel and industrial chemicals to pharmaceutical alcohol.

The health values of Phoenix dactylifera (dates): A review

Dates are one of the most prominent types of fruits spread in the Middle East and North Africa and have been an essential part of thediet of the Arab world since ancient times. It has been called “nature’s candy” due to its sweet taste, which is attributed to its high sugarcontent. Date fruits have great nutritional and therapeutic value, due to the variety of elements they contain, as they contain a goodpercentage of fiber, vitamins, and minerals, especially magnesium, potassium, selenium, calcium salts, and fatty acids (lauric, linoleic,palmitic, and citric acid) as well as an excellent source of antioxidants (flavonoids, polyphenols, and carotenoids) which offer significanthealth-promoting and disease-fighting benefits. In this review, the origin of the date palm fruits was studied, and the nutritional valuewas compared between three types of dates (Ruthana, Sukari, Ajwa) in terms of the nutritional components and the therapeutic values,which is proven by several studies. The nutritional content of date fruits; sugars, beneficial fats, nutritious proteins, and phytochemicalswas reviewed. The health benefits of date fruits include their ability to inhibit free radicals, anti-inflammatory activity, cytotoxicity oncancer cell lines, and the applications for its use as a functional food were indicated. The exact mechanisms still need further researchand exploration, specifically, Ruthana fruits as this type of available literature showed a research gap that could be investigated

Evaluation of Different Insecticides Against Lesser Date Moth, Batrachedra amydraula Meyrick, 1916 (Lepidoptera: Batrachedridae) under Field Conditions

Evaluation of Different Insecticides Against Lesser Date Moth, Batrachedra amydraula Meyrick, 1916 (Lepidoptera: Batrachedridae) under Field Conditions

Effect of Date Palm (Phoenix dactylifera) Fruits Consumption on Birth Delivery Outcome: Meta-Analysis

Background: The choice of cesarean section continues to increase in the world and is expected to continue to increase over the coming decades, which is almost a third of all deliveries in 2030. The benefits of dates for improving labor processes are considered to increase vaginal deliveries and also reduce the frequency of cesarean sections with the aim of reducing bigger complications. This study aims to analyze and estimate the influence of the consumption of dates on labor outcomes. Subjects and Method: This study is a systematic review and meta-analysis with PICO, P: Pregnant women, I: consumption of dates, C: No consumption dates, O: Bishop's score, length of labor, and postpartum hemorrhage. Data collection was obtained from databases, namely: PubMed, Science Direct, Springer Link, and Google Scholar. Keywords used “date fruit” OR “date palm” OR “phoenix dactylifera” AND “labor” AND “delivery”, AND “oxytocin” AND “hemorrhage”. The inclusion criteria used were full papers in English with the design of randomized controlled trials in 20002022. This study was analyzed using RevMan 5.3 software. Results: Meta-analysis was conducted on 4 primary studies from several countries such as Iran, Malaysia, and Saudi Arabia with a sample size of 603 pregnant women. The meta-analysis concluded that there was an effect of date consumption on cervical dilatation upon admission to the hospital (SMD= 0.73; CI 95%= 0.09 to 1.38; p=0.030). Consumption of dates also has an effect on the duration of active 1st stage labor (SMD= 0.46; CI 95%= 0.82 to 0.09; p= 0.010). Consumption of dates has a weak effect on the length of stage II labor (SMD= 0.05; 95% CI= 0.88 to 0.78; p= 0.910). In addition, the consumption of dates also has a weak effect on the length of stage III labor (SMD=0.16; CI95%=0.46 to 0.79; p=0.610). Conclusion: Consumption of dates has an influence on labor outcomes. Keywords: phoenix dactylifera, dates, labor, postpartum hemorrhage, Bishop score Correspondence: Ratna Purwani Siwi. Masters Program in Public Health, Universitas Sebelas Maret. Jalan Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia. Email: ratnapsiwi@yahoo.com. Mobile: 085602389598. Indonesian Journal of Medicine (2023), 08(01): 79-91 https://doi.org/10.26911/theijmed.2023.08.01.08

Optimizing the extraction of phenolic antioxidants from date palm fruit by simplex-centroid solvent mixture design

Date palm (Phoenix dactylifera L.) fruits are rich in various bioactive compounds, such as phenolic acids, hydroxycinnamates, flavonoid glycosides, coumarins, alkaloids, and proanthocyanidin oligomers. The focus of this study was to develop a simplex-centroid mix design method to identify the most suitable mixture of solvents (water, acetone, and methanol) to extract bioactive compounds from date fruits. Three extraction solvents (water, methanol, and acetone) were investigated during this study to determine, total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (free radical DPPH) and ferric reduction ability (FRAP). The results showed that polar mixtures were effective in extracting antioxidant phenolics. The optimum solvent for extraction was Binary mixture water-acetone (50%) presenting TPC, TFC, DPPH and FRAP values of 502.88 mg GAE.100 g-1 DW, 206.23 mg QE.100 g−1 DW, 77 0.01% and 1688.66 μmol.100 g−1 respectively. The results also confirmed a strong correlation between the amount of polyphenols in a given extract and the antioxidant activities observed in the DPPH and FRAP assays. This study presents a pragmatic and efficient way to choose a solvent combination to extract polyphenols from date palm fruits.

Anatomical Assessment of Skin Separation in Date Palm (Phoenix dactylifera L. var. Mejhoul) Fruit during Maturation and Ripening Stages

Skin separation (puffiness) is a critical physiological disorder that significantly reduces the date palm (Phoenix dactylifera L.) fruit’s visual appearance and quality. The objectives of this study were to analyze fruit anatomy in skin-separated and normal date palm (var. Mejhoul) fruit and to assess the microclimatic conditions (temperature and relative humidity) during the fruit developmental stages and their association with skin separation. Fruit anatomy analysis was carried at different growth stages (Kimri-Khalal, Khalal, Rutab and Tamr) for two growing seasons. In addition, microclimatic conditions (specifically, temperature, and relative humidity) as well as soil moisture content were assessed across the study period 2020 and 2021. The anatomical analysis of the date palm fruit revealed that the exocarp or skin (cuticle, epidermis, hypodermis) thickness were quite similar across the developmental stages and over the study period (2020, 2021). Conversely, significantly more sclereid cells were found in skin-separated fruit (compared to normal fruit) at the Tamr stage. At that stage (Tamr), the percentage increase in sclereid cell number in skin-separated fruit ranged from 34–183%, sclereid clusters number 23–92%, cluster area 37–206%, and cluster perimeter 25–64%, as compared to normal fruit. In addition, sclereid cells at skin-separated areas of the fruit were clustered together, forming a chain of aggregates underneath the fruit skin, and were closer (42–50%, than normal) to the cuticle, leading to the partial separation of the exocarp from the fruit mesocarp. Although the weather data were similar across the growing seasons (temperatures, 33–34 °C; relative humidity, 45–46%), skin separation occurred in 14–15% of total fruit in 2020 and 30–34% in 2021. Therefore, we believe that skin separation disorder is not as heavily weather dependent as it seems to have been thought. In conclusion, changes in microclimate conditions were not the conclusive component for inducing the skin separation physiological disorder in date palm fruit. Further studies are required to identify the main factors that stimulate the development of sclereid clusters aggregates and the potential of agricultural practices to reduce skin separation.