Dangerous Pests Research Articles

Potential Ecological Distribution of the Beetle Agrilus mali Matsumura (Coleoptera: Buprestidae) in China under Three Climate Change Scenarios, with Consequences for Commercial and Wild Apple Forests.

The apple jewel beetle (AJB), Agrilus mali Matsumura (Coleoptera: Buprestidae), is a dangerous pest of commercial apple orchards across China, the largest apple production country in the world, and has recently become invasive in the Xinjiang Uygur Autonomous Region (XUAR) of northwestern China, where wild apple forests also occur. This pest poses a serious threat to apple production and wild apple forests throughout the world. Global warming is expected to change the geographical distribution of A. mali in China, but the extent of this is unknown. Based on empirical data from 1951 to 2000, a MaxEnt model was used to forecast the ecological distribution of A. mali under three different climate scenarios projected in the fifth report of the Intergovernmental Panel on Climate Change. The results showed that the most important variables were the maximum temperature of November, precipitation in January, and minimum temperatures in April. Under all climate scenarios, the forecasted suitable regions for A. mali in China will expand northward in the 2050s and 2070s. The forecasted highly suitable regions will be 1.11-1.34 times larger than they are currently, and their central distributions will be 61.57-167.59 km further north. These findings suggest that the range and damage caused by A. mali in China will increase dramatically in the future. Monitoring and management measures should be implemented urgently to protect both the commercial apple industry and wild apple resources.

Development and validation of a rapid loop-mediated isothermal amplification assay for the detection of Chrysomyxa and characterization of Chrysomyxa woroninii overwintering on Picea in China

Chrysomyxa rusts cause significant damage to spruce in both natural forests and plantations. Particularly, Three Chrysomyxa species, Chrysomyxa deformans, Chrysomyxa qilianensis, and Chrysomyxa rhododendri, listed as National Forest Dangerous Pests in China, have severely affected many economically and ecologically important spruce native species in China. Also, Chrysomyxa arctostaphyli, an important plant quarantine fungus, causes a damaging broom rust disease on spruce. Therefore, rapid, and efficient detection tools are urgently needed for proper rust disease detection and management. In this study, a sensitive, genus-specific loop-mediated isothermal amplification (LAMP) assay targeting the ITS-28S rRNA region was developed to detect the presence of Chrysomyxa in spruce needle and bud samples. After optimization and validation, the LAMP assay was found to be sensitive to detect as low as 5.2 fg/µL DNA, making it suitable for rapid on-site testing for rust infection. The assay was also specific to Chrysomyxa species, with no positive signals from other rust genus/species. The application of LAMP in the early detection of rust infections in spruce needles and buds was investigated, and spatial colonization profiles as well as the means of overwintering of Chrysomyxa woroninii in infected buds and branches were verified using the LAMP assay. This LAMP detection method will facilitate further studies on the characteristics of the life cycle and inoculation of other systemic rusts.

Scientific achievements in Siberian agriculture and their development

A historical review of the formation and development of scientific farming in Siberia is given. The main achievements of the leading research institutes of the region are highlighted. The contribution of Siberian scientists to the development of scientific farming, including its constituent directions, is shown. It presents almost 100 years of development of research on agriculture - from the Omsk experimental farm (XIX century), agricultural experimental stations - to modern federal scientific centers. The evolution of farming from individual crop cultivation techniques to the formation of zonal and then adaptive-landscape farming systems and modern agro-technologies is demonstrated. Examples of completed scientific developments on farming, ready for implementation in production, the number of which runs into thousands, are given. Implementation of knowledge-intensive agro-technologies guarantees grain production in Siberia up to 25-30 million tons, sustainable development of farming, stable food supply and high return on non-renewable resources. The evolution of plant protection from individual methods against the most dangerous pests to the formation of integrated systems that take into account the phytosanitary state of agrocenoses and agro-ecological conditions of agricultural crops cultivation is shown. The most important techniques to optimize the relationship between the crop and pests are the selection of plant species and varieties resistant to their effects, optimization of mineral nutrition of plants and sowing dates. At present, the development of scientific farming is associated with the design of adaptive-landscape farming systems, selection and support of agro-technologies, rational use of land resources, improvement of approaches to the conservation and reproduction of soil fertility, soil treatment systems, fertilizers and integrated plant protection, formation of adaptive crop rotations, research of technological and biological processes in the cultivation of crops, use of GIS-technologies, remote sensing, and the use of remote sensing.

Pathogenicity Efficacy of Entomopathogen Fungus Beauveria bassiana Against In Vitro Rice Stem Borer (Scirpophaga innotata)

White rice stem borer (Scirpophaga innotata) is one of the dangerous pests for rice (Oryzae sativa) because it attacks rice stems that can cause 50% loss of total production. The purpose of this study was to determine the pathogenicity of Entomopathogen Fungus Beauveria bassiana at different conidia dilutions (J), namely J1 (105), J2 (106), J3 (107), J4 (108), and J5 (109). The mortality of S. innotata larvae was tested with the density of B. bassiana of 2.08 × 106 conidia/ml. The effect of B. bassiana on the success transformation of S. innotata larvae to pupae for 14 observation day was also observed at the same B. bassiana density. This research resulted in the optimal pathogenicity of B. bassiana at dilution of 107 (treatment J3) which caused 100% death and 100% successful pupation. The lowest treatment was J5 (109 dilution) with a mortality rate of 50% and a successful pupation of 20%. The results of the probit analysis showed that the fastest average lethal time (LT50) value was found in treatment of 107 (J3) which reached 11.11 h. Keywords: Beauveria bassiana, Pathogenicity, Probit analysis, Scirpophaga innotata.

Efficacy of Some Aquatic Plant Extracts on the Khapra Beetle <i>Trogoderma Granarium</i> Everts

Khapra beetle is one of the dangerous pests that infests fields and warehouses, and using safe alternatives such as plant extracts is one of the options. The efficacy of aqueous extracts of Achillea fragrantissima and Chenopodium album (5, 10, 15%) at different exposure durations (48, 96, and, 144 hr) has been evaluated in this study. Mortality of fifth-instar larvae and adults was observed, and it was shown that an exposure period of 144 hr was superior with hot and coldwater extracts of C. album. The results also showed that maximum mortality of fifth instar larva (65,000 and 92,405%), respectively, was at a concentration of 15% for the cold and boiling water extracts. Mortality of adults was maximum with cold and hot water extracts of the C. album, at an exposure period of 144 hr, as well as at 15% (36,809 and 55,671%, and (51,299 and 74,671%, respectively). Thus the results showed that C. album extract was superior, there existed a significant effect of the interaction between (extract type, concentration, and treatment duration) on the mortality of larvae and adults.

Investigating biological performance, oviposition and diet preference of the twospotted spider mite feeding on various melon genotypes

The twospotted spider mite, Tetranychus urticae Koch, is one of the most dangerous pests for many crops worldwide, including the melon. Here, we raise the hypothesis that the resistance of melon genotypes to the spider mite is associated with the morphological characteristics of the leaves. We determined (i) the feeding preference and oviposition suitability of T. urticae on four melon genotypes, (ii) various biological parameters and population growth rate (ri) of T. urticae on different melon genotypes, (iii) the leaf morphology of the melon genotypes, and (iv) the relationship between the leaf characteristics and resistance to T. urticae. In no choice tests, lower numbers of adults and eggs of T. urticae were found in the genotypes CNPH 06–1047-343, CNPH 11–1071-43, and CNPH 06-1047-341 compared to control (Goldex). Individuals that fed on CNPH 06–1047-343, CNPH 06–1047-341, and CNPH 11–1071-43 genotypes had a longer egg incubation time, longer development time, lower immature viability, longer time to reach adult stage (egg–adult) and lower instantaneous rate of increase compared to control. These findings are in line with the fact that these three genotypes had thicker epidermis and greater mesophyll thickness than control genotype “Goldex”. Even the genotypes CNPH 06–1047-343, CNPH 06–1047-341, and CNPH 11–1071-43 had higher trichome densities, this morphological characteristic was not related with the resistance to T. urticae. CNPH 06–1047-343, CNPH 06–1047-341, and CNPH 11–1071-43 showed antibiosis and antixenosis against T. urticae and that resistance of the melon to T. urticae wss associated with the greater thickness of the leaf epidermis and mesophyll.

First Report on Banana Weevil, Cosmopolites sordidus (Germar 1823) (Coleoptera: Curculionidae), an Exotic Economically Important Pest from Saudi Arabia.

We report the first record of the occurrence of the banana weevil, Cosmopolites sordidus (Germar, 1823) (Coleoptera: Curculionidae), an economically important pest of bananas (Musa spp.), from Fifa Mountains in Saudi Arabia. Moreover, we recorded the first observation of damage caused to bananas by C. sordidus in a banana farm in Jazan Province, southwestern Saudi Arabia, in March 2022. Molecular characterization using DNA sequences of the mitochondrial COI gene confirmed the morphological identification of C. sordidus. This discovery is considered a warning notice to prevent the potential establishment and spread of this dangerous pest in the banana cultivation regions in Saudi Arabia. Therefore, it is recommended that detection and monitoring of banana weevil should be undertaken in Saudi banana farms in order to restrict the dissemination of this weevil to other banana cultivation areas.

Forecasting the phytosanitary state of agrocenoses: innovation developments

Goal. Development and implementation of new theories, methods, technologies and models that would allow more reliable forecasting of the development of pests and pathogens of agricultural crops.
 Methods. Analysis of innovative development of the Institute of Plant Protection of the National Academy of Agrarian Sciences of Ukraine and other institutions of the Scientific and Methodological Center «Protection of Plants» for 2001—2020. Isolation of those relating to the problem forecasting the development of pests and pathogens of plant diseases in agrocenoses of Ukraine.
 Results. The concept of formation of the phytosanitary state of crops and plantations under the conditions of climate change has been developed. The theory of the dynamics of the number of locusts and the prediction of emergency situations in the agricultural sphere of Ukraine has been developed. The widespread use of pheromone traps for diagnosing the phytosanitary state of agrocenoses of Ukraine is recommended. The cyclicity of outbreaks of mass reproduction of the Anisoplia austriaca beetle and the European corn borer was established, depending on the amount of solar activity. The possibility of applying GIS-technologies for the analysis of long-term data was studied. Predictive computer programs (models) of harvest shortages of rapeseed, sunflower, corn, sugar beet, and winter wheat from a complex of pests have been developed. An algorithm for the application of information technology programs for the analysis of multi-year dynamics of the phytosanitary state of agrocenoses has been developed. The role of the developed computer model of the development of leaf spots of grain crops in determining the moment for the most effective application of fungicides has been established. Methods of forecasting the development of pathogens of grape diseases in the South of Ukraine have been developed. A system of forecasting the phytosanitary state of agrocenoses of Ukraine in the conditions of the year and planning the implementation of protective measures has been developed.
 Conclusions. Innovative developments of the Scientific and Methodological Center «Plant Protection» make it possible to solve the following issues at a modern level: forecasting changes in the agrosphere based on the analysis of a long-term database of hydrothermal conditions and indicators of the phytosanitary state of agrocenoses; prevention of emergency situations in the agricultural sector on the basis of a modern monitoring system using GPS-navigation and the development of regulations for carrying out protective measures; forecasting the development of dangerous pests and diseases of agricultural crops; forecasting crop failures and determining the economic feasibility of chemical plant protection; forecasting the appearance and spread of quarantine pests.

Optimal control of red palm weevil model incorporating sterile insect technique, mechanical injection, and pheromone traps

The red palm weevil is one of the most dangerous pests affecting date palm trees in the Gulf region and the Middle East. This paper investigates the control of the red palm weevil (RPW) in date palms using several techniques, namely stem injection, sex pheromone traps, and the Sterile Insect Technique. A mathematical model describing the spread of the red palm weevil is presented, and the boundedness of the solution for the RPW system is studied. The conditions for local stability, forward bifurcation, and Hopf bifurcation are also obtained. Additionally, this research highlights the significance of optimal control in managing RPW and simulates the model using the Forward-Backward Sweep numerical method. It is observed that the parameters of the sterile insect technique, the pheromone trap, and the mechanical injection play important roles in controlling the RPW. Mechanical Injection may be more effective in controlling the palm weevil compared to the other two methods.

Influence of Chabazite Zeolite Foliar Applications Used for Olive Fruit Fly Control on Volatile Organic Compound Emission, Photosynthesis, and Quality of Extra Virgin Olive Oil.

The olive fruit fly (Bactrocera oleae Rossi) is the most dangerous pest of olive fruits and negatively influences the chemical and sensory quality of the oil produced. Organic farms have few tools against this pest and are constantly looking for effective and sustainable products such as geomaterials, i.e., zeolite. Since a particle film covers the canopy, a study was carried out on the olive tree's responses to zeolite foliar coating. The tested treatments were natural zeolite (NZ), zeolite enriched with ammonium (EZ), and Spintor-Fly® (SF). EZ was associated with higher photosynthetic activity with respect to the other treatments, while no differences were found between SF and NZ. Foliar treatments affect the amount of BVOC produced in both leaves and olives, where 26 and 23 different BVOCs (biogenic volatile organic compounds) were identified but not the type of compounds emitted. Foliar treatment with EZ significantly affected fruit size, and the olive fruit fly more frequently attacked the olives, while treatment with NZ had olives with similar size and attack as those treated with Spintor-Fly®; no difference in oil quantity was detected. Oil produced from olives treated with NZ presented higher values of phenolic content and intensities of bitterness and spiciness than oils from those treated with EZ and SF. According to the results of this study, using zeolite films on an olive tree canopy does not negatively influence plant physiology; it has an impact on BVOC emission and the chemical and sensory characteristics of the oil.

Tomato pest recognition using convolutional neural network in Bangladesh

The tomato is one of the most popular and well-liked veggies among Asians. It is interesting to note that in Bangladesh, it is the second most significant vegetable consumed. Moreover, tomato is served not only as a vegetable, but it is also served as sauce, jam, etc., and used in making different types of cuisines. But the fact is due to the pests, thousands of tons of tomatoes are harmed every year in Bangladesh. The production of tomatoes in Bangladesh is harmed by a number of dangerous pests. We develop a solution to recognize pests at an early stage. Five different pest types, including aphids, red spider mites, whiteflies, looper caterpillars, and thrips, have been studied in this research. To identify tomato pests, we curated image datasets from online and offline repositories and processed them using a convolutional neural network (CNN) model. We used features from CNN layers for three machine learning algorithms: Random Forest (RF), support vector machine (SVM), and K-Nearest Neighbors (K-NN). This comprehensive approach allowed a thorough comparison of these algorithms in tomato pest recognition. For recognizing tomato pests, our methods generate excellent results. The accuracy of our experiment is 95.49% which indicates the successful completion of the experiment.

A ScWIP5 gene confers fall armyworm resistance by reducing digestive enzyme activities in sugarcane.

The fall armyworm, Spodoptera frugiperda, is one of the most dangerous pests to various crops. As the most crucial sugar crop, sugarcane is also constantly threatened by these pests. Plant wound-induced proteinase inhibitors (WIP) are natural defense proteins that play important roles in the defense system against insect attack. Breeding for resistance would be the best way to improve the variety characteristics and productivity of sugarcane. Screening and verification for potential plant endogenous insect-resistant genes would greatly improve the insect-resistant breeding progress of sugarcane. A sugarcane WIP5 gene (ScWIP5) was up-regulated 536 times after insect feeding treatment on previous published transcriptome databases. ScWIP5 was then cloned and its potential role in sugarcane resistance to fall armyworm evaluated by construction of transgenic Nicotiana benthamiana. The toxicity of ScWIP5 transgenic N. benthamiana to fall armyworm showed lower weight gain and higher mortality compared to wild-type N. benthamiana feeding group. Furthermore, the concentration of JA and NbAOC, NbAOS, and NbLOX from the Jasmin acid biosynthesis pathway was significantly induced in ScWIP5 transgenic N. benthamiana compared to the control. In addition, digestive enzyme actives from the insect gut were also evaluated, and trypsin and cathepsin were significantly lower in insects fed with ScWIP5 transgenic N. benthamiana. These results indicate that ScWIP5 might enhance insect resistance by increasing JA signal transduction processes and reducing insect digestive enzyme activities, thus impacting insect growth and development. © 2023 Society of Chemical Industry.

Selection of Cotton Varieties in a Competitive Nursery in the South of Kazakhstan

The Turkestan region is a cotton-growing zone in the south of Kazakhstan, which is the northernmost cotton-growing zone in the world. 115-125 thousand hectares of medium-staple cotton (Gossypium hirsutum L.) are cultivated here annually, of which 80-85 thousand hectares are sown in the Maktaaral and Zhetysay districts. This region is highly susceptible to salinity, drought, invasion of dangerous pests (cotton budworm, beet borer, spider mites, aphids), and diseases (fusarium blight (wilt), gummosis). Considering the high salt content in the arable soil horizon, the aridity of the climate of the Turkestan region are the main limiting factor of the region, and selection and genetic methods is the most effective and economical way to reduce their negative impacts on cultivated vegetation, then research work on the study and creation of new resistant cotton varieties in these soil and climatic conditions are relevant. Considering all the above problems, scientists of the Agricultural Experimental Station of Cotton and Melon Growing LLP have set themselves the goal of creating heat- and drought-resistant, precocious cotton varieties with high productivity, resistance to salinization, diseases, pests, possessing high technological qualities as the yield and quality of fiber of types III-IV, based on previously obtained ones during hybridization of intraspecific and interspecific lines of families. For 30 years, scientist-breeders of Kazakhstan have created 13 varieties of medium-fiber cotton, of which 8 are approved for use in the Turkestan region in the Republic of Kazakhstan. The research method is based on hybridization, multiple selection, and testing of offspring by the half method, according to the full diallel scheme (first Griffing model for F1). The created seven varieties PA-3031, PA-3044, M-4005, M-4007, M-4011, Bereke-07, Myrzashol-80, and M-4017 are zoned for more than 92% of the acreage in cotton-growing farms in the south of Kazakhstan, for the variety M-5027, which is resistant to pests, the patent has been issued 2021 and in 2022 it is planned to enter it into the register of approved varieties.

Benzyl alcohol, benzyl benzoate and methyl benzoate as bio-insecticides against dried bean beetle Acanthoscelides obtectus (Coleoptera: Tenebrionidae)

Acanthoscelides obtectus is most dangerous pest of preserved beans. The current study used benzyl alcohol (BA), benzyl benzoate (BB), and methyl benzoate (MB) to control A. obtectus. Contact and fumigant toxicity tests were performed to evaluate the efficacy of the used compounds. The contact toxicity assay revealed a significant mortality rate with LC50 reached at low concentrations of 0.57, 0.68, and 0.94 μL/g for BA, BB, and MB, respectively. BA possesses significant fumigant toxicity and outperforms MB and BB, with an LC50 achieved at concentration of 8.91 μL/L. In comparison to the control group, BA, BB, and MB have anti-feedant efficacy against A. obtectus. Furthermore, even at a low concentration of 0.5 μL/g, these compounds induced a significant suppression of A. obtectus F1 progeny production. The BA and BB activities persisted until day 5 after treatment while MB activity sustained for 3 days only. These compounds significantly inhibited acetylcholinesterase, decrease in the level of reduced glutathione (GSH), and elevated malondialdehyde (MDA). In conclusion, BA, BB, and MB are particularly promising choices for controlling the bean beetle A. obtectus because they are low in cost, of natural origin, and the European Food Safety Authority (EFSA) approved their use in animal feed.

Key factors in the successful adaptation of the pest <i>Corythucha ciliata</i> Say in the northern subtropics of the Black Sea coast

Aim. To identify a complex of factors in the successful adaptation of the sycamore lace bug Corythucha ciliata Say for the development of methods for regulating its numbers of and effective biological control measures against this dangerous pest. Material and Methods. The research was carried out by studying scientific publications, analysing the dynamics of climate change in the Lazarevsky district of Sochi for 15 years and studying the reporting data of the Lazarevskaya Experimental Plant Protection Station, Branch of the Federal Research Center of Biological Plant Protection for the period 2008 to 2022. Results. 5 key factors of successful adaptation of Corythucha ciliata to new invasion regions have been identified: the factors of food and climatic adaptation, the factor of interaction with natural enemies, the factor of resistance to entomopathogenic organisms and the factor of adaptation to anthropogenic load. The main conditions and possible vectors of further invasion of sycamore lace in new regions have been determined. Conclusion. The development of effective methods of biological control of the invasive pest Corythucha ciliata Say should be based taking into account the totality of qualitative characteristics of the pest's living conditions. The sycamore lace bug Corythucha ciliata Say effectively builds new trophic connections, without prejudice to its population, adapts not only to new climatic conditions, but also to their changes. Natural entomophages and entomopathogens do not significantly affect the development of the Corythucha ciliata population. The pest has adapted to survival in conditions of high anthropogenic load and successfully uses it to invade new regions.

Field evaluation of the efficacy of copper nanoparticles against mites associated with orange trees.

Phytophagous mites are dangerous pests, causing economic losses to the world's crops. Nanotechnology is a promising field for pests and disease management, and enhancement of agricultural productivity. The aim of the study was to evaluation of the effectiveness of copper nanoparticles (CuNP) against all stages of eriophyid mite, the citrus rust mite, Phyllocoptruta oleivora (Ashmead) (Acari: Eriophyidae) and tetranychid mite, the citrus brown mite Eutetranychus orientalis (Klein) (Acari: Tetranychidae), and Tenuipalpid mite, the false spider mite, Brevipalpus obovatus Donnadieu (Acari: Tenuipalpidae). This includes its impacts on predacious mites, Amblyseius swirskii Athias-Henriot and Euseius scutalis (Athias-Henriot) (Acari: Phytoseiidae), on orange trees under field conditions. Five different concentrations of copper nanoparticles (40, 80, 160, 240, and 320 ppm), as well as the control (well water) were examined. The obtained results indicated that the mortality rate of both phytophagous and predacious mites was associated with an increase in the concentrations of copper nanoparticles. Copper nanoparticles were significantly effective in killing P. oleivora, E. orientalis and B. obovatus with minimal effects on A. swirskii and E. scutalis. The mortality percentage was 15.24, 20.32, 46.32, 78.97 and 86.37% for P. oleivora, 6.87, 9.86, 28.91, 56.30 and 77.52% for E. orientalis and 8.38, 23.50, 48.83, 68.80 and 84.08% for B. obovatus while the mortality percentage was 0.00, 0.56, 5.83, 9.91 and 15.19% for A. swirskii and 0.44, 3.96, 6.93, 8.63 and 21.39% for E. scutalis one week after exposure to 40, 80, 160, 240 and 320 ppm of copper nanoparticles, respectively. Moreover, the results showed that copper nanoparticles caused a reduction in the percentage of eggs hatching. The percentages of larvae hatching from eggs were 96.29, 80.00, 64.13, 45.66 and 32.17% for P. oleivora, 97.38, 83.28, 69.41, 48.01 and 35.29 for E. orientalis and 96.60, 76.92, 56.38, 40.55 and 33.28% for B. obovatus one week after exposure to copper nanoparticles at 40, 80, 160, 240 and 320 ppm respectively, compared with the control (well water). According to the results, the use of copper nanoparticles significant effect on reducing the population of phytophagous mites associated with orange trees, with low detrimental effects on predatory mites.

Study of the resistance of economically important pine species to dangerous pests on the Absheron Peninsula

The main goal of the research is to study the resistance of widely used pine species in the landscaping of the Absheron Peninsula to serious dangerous pests, to select relatively resistant species, and to recommend them for landscaping farms. At the initial stage of scientific research, phytosanitary monitoring of various types of pine trees cultivated on the peninsula was carried out, dangerous organisms were studied, analyzed, and identified (2020-2023). As a result of the research, it was established that dangerous microorganisms that are often found in economically important pine species in the studied region: pine bark beetle, sub-bark pine bug, pine scale insect, and the causative agent of tar cancer, cause serious damage to the lifestyle and productivity of plants. This is explained by the fact that harmful organisms cannot cause serious harm to the plant due to the activation of protective and regenerative processes inside the plants. Thus, taking into account the resistance of P. eldarica to other unfavourable soil and climatic factors of the peninsula, including the mentioned dangerous pathogenic and harmful insects, in comparison with other species, it is advisable to widely use it as the dominant species in the region’s landscaping.

Utilization of Shrimp Shells Waste and Golden Snails to Increase the Yield of Shallots

Shallots are high economic value commodities. The chemical fertilizers alone in onion cultivation can treat to agricultural sustainability. Environmentally friendly alternative fertilizers are urgently needed. One of them is Local Microorganism (LMO) which is developed from waste/natural materials, such as shrimp shells and golden snails which are available in nature. Shrimp shell contains nutrients N, P, K, C, Mg, and Fe. Golden snail is dangerous pest for rice plants, but it can be used as organic fertilizer. This study aims to determine effect of giving LMO of shrimp shells and golden snail for shallots growth and yield. The experiment used randomized block design with eight treatments, four replications, namely without LMO, 1.2 g plant-1 NPK fertilizer; 250 mL, 300 mL, and 350 mL shrimp shell LMO; LMO golden snail 250 mL, 300 mL, and 350 mL. The results showed that the provision of local microorganisms of shrimp shells and golden snails was able to increase shallot growth and yield, which was indicated by an increase in plant height, number of leaves, fresh weight, and dry weight of tubers per plant. The increase in tuber dry weight ranged from 130 % to 239 % (2.3 times to 3.4 times higher than control).

Genetic Diversity of Barley Accessions from East Asia for Greenbug Resistance.

The greenbug, Schizaphis graminum, is a dangerous pest of barley and other grain crops in the south of Russia. An effective and environmentally friendly way to control this insect is to cultivate resistant varieties. The differential interaction between the phytophage and host plants necessitates the search for new donors of resistance. Seven hundred and seventy-eight accessions of barley from East Asian countries (313 from China, 450 from Japan, and 15 from Nepal) were evaluated for greenbug resistance. The Krasnodar population of the insect and clones isolated from it were used in the experiments. Forty heterogeneous accessions were identified, in which plants with a high level of resistance to the aphid were found. As a result of damage assessment by the 108 S. graminum clones of 11 lines selected from heterogeneous accessions, 52 insect virulence phenotypes were identified. Experiments with aphid test clones showed that all 11 lines possess diverse greenbug resistance alleles, which differ from the previously identified Rsg1, but their efficiency is low. The frequency of clones virulent to ten lines and the cultivar Post (a carrier of the Rsg1 gene) varies from 60.4% to 98.0%. The exception is line 15903, which is resistant to the aphid population and protected by one dominant gene. The high resistance of other lines against a part of the natural population of S. graminum is also under oligogenic control. Lines 15600 and 16190 each have one dominant resistance gene, and line 28129 is protected by two genes, the dominant and recessive ones. A recessive resistance gene is presumably present in line 15600. Lines 16237/1 and 16237/2, isolated from the same collection accession, each have one dominant gene effective against individual aphid clones. The loss of effectiveness of distinctly manifested resistance genes causes the expression of previously masked genes with a weak phenotypic manifestation, which differentially interact with insect genotypes.

Geospatial Analysis For Determination Of Preferential Soil Conditions For The Desert Locust Oviposition

Understanding the thresholds of influencing parameters that favor the habitability of dangerous pests like desert locusts (Schistocerca gregaria) can aid in early detection and eradication using control operations. The life cycle of the desert locust and its behavioral changes are associated with the weather patterns and the region’s ecosystem settings. This study attempts to retrieve the preferential soil conditions like texture and moisture at the surface and subsurface levels for egg-laying by desert locusts. Towards this, Locust Hub, a comprehensive database of desert locusts maintained and disseminated by the Food and Agriculture Organisation under the Locust Watch program, was used to identify breeding site locations for 2017-2021. In this research, we extracted sand-silt-clay percentage at these breeding sites using SoilGrids ver. 2.0 from the World Soil Information Service database facilitated by International Soil Reference and Information Centre. Similarly, soil moisture conditions extracted from Level-4 data products of the Soil Moisture Active Passive mission for all these breeding sites aided in essaying the optimal soil conditions for the desert locust’s oviposition. This study’s results confirm the sand percentage in a broad range of 55-70% (for 90% of samples), followed by a narrow range of silt and clay with 19-24% and ~14-20%, respectively. Our study reveals that female desert locusts prefer sandy loam-textured soils for oviposition. The preferred range of soil moisture at the surface and subsurface resulted in 5-10% and 10-20%, respectively. These results confirm that dampness is required at the surface soil for initiating the oviposition by female desert locusts. Results from this research can aid in the early identification of breeding grounds during desert locusts’ invasion period.