Cytokine Levels In Patients Research Articles

Analysis of Optimizing Effects of Dexmedetomidine and Oxycodone on Cytokine Levels in Patients with Laparoscopic Radical Gastrectomy

This study was designed to analyze the effects of dexmedetomidine combined with oxycodone in patients underwent laparoscopic radical gastrectomy for stomach carcinomas. A total of one hundred and twenty (120) patients were recruited and divided into A, B and C groups, using a random number table method, 40 cases in each group. After surgery patients in group A received oxycodone alone, patients in group B received dexmedetomidine combined with oxycodone and patients in group C received dexmedetomidine alone respectively. The Visual Analog Scale score and Ramsay sedative score at 2h, 6h, 24h and 48h after surgery were observed and the effective compressions number of controlled intravenous analgesia was documented. The incidence of side effects was recorded. TNF-α, CRP and IL-6 were evaluated by enzymelinked immunosorbent assay on the day before surgery and in the morning of 1st and 3rd d after surgery. VAS scores in group B were lower than group A and C, the effective compressions number of CIA in group B was less than group A and C, the occurrence of adverse reactions in group B was lower than group A and group C. The postoperative levels of TNF-α, CRP and IL-6 at 72h in group B were lower than group A and C. Dexmedetomidine combined with oxycodone showed improve sedative and analgesic effects with fewer adverse reactions. It can lessen the release of inflammatory cytokines of TNF-α, CRP and IL-6 in the body and has certain clinical application value.

A Novel Systemic Inflammatory Score Combined With Immunoinflammatory Markers Accurately Reflects Prognosis in Patients With Esophageal Cancer.

To establish a novel systemic inflammatory score (SIS) combined with neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and C-reactive protein/albumin ratio (CAR) and to validate its prognostic value and relation with serum cytokine levels in patients who underwent esophagectomy for esophageal cancer (EC). Preoperative NLR, PLR, and CAR were evaluated in 102 patients undergoing esophageal resection for EC from 2009 to 2014. Receiver operating characteristic (ROC) curves censored for 5-year survival were plotted to determine the cutoff values of each measure. Each measure was scored 1 if it was above the cutoff value (NLR >3.12, PLR >230, and CAR >0.085) and scored 0 if it was below that. The SIS was defined as the sum of these values and was divided into the two groups: High SIS (SIS=2-3) and low SIS (SIS=0-1). Univariate and multivariate analyses were used to determine the prognostic significance. The area under the ROCs (AUROC) was compared to verify the discriminative power of survival prediction. In addition, we analyzed the relationship between SIS and perioperative serum interleukin (IL)-6 and IL-10 levels. In the clinicopathological findings, only tumor depth was significantly related to SIS (p=0.004). At 0.732, the AUROC of SIS was the highest (NLR=0.618, PLR=0.545), and CAR=0.712). The high-SIS group had a significantly poorer prognosis than the low-SIS group (p=0.011). SIS was identified as an independent prognostic factor in the multivariate analysis (hazard ratio=1.96, 95% confidence intervaI=1.11-3.41, p=0.020). The preoperative serum interleukin-6 level was significantly low (p=0.046) and postoperative serum interleukin-10 level was significantly high in the high-SIS group (p=0.047). SIS was a superior predictor of prognosis compared with existing immunoinflammatory markers and closely reflected the fluctuation of peripheral inflammatory cytokines in patients with EC.

ROLE OF PROINFLAMMATORY CYTOKINES IN PATHOGENESIS OF ARTHROPATHIES IN PATIENTS WITH DIABETES MELLITUS

The aim: Of our work was to study the level of proinflammatory cytokines in patients with diabetic arthropathy and to investigate their possible effect on the development of this complication. Materials and methods: 118 patients were examined, which were divided into groups by type of diabetes, the presence and severity of diabetic arthropathy. The content of IL-1, TNF-α, IL-6 and receptors to S IL-6-R in serum was determined by immunoassay. Results: In patients with diabetic arthropathy, levels of TNF-α (with type 1 diabetes 44.5%, type 2 diabetes 42.9%) and IL-6 (with type 1 diabetes 52.1%, with diabetes 2 types by 64.4%) significantly increased. There is a direct correlation between the severity of joint damage and the level of TNF-α and IL-6. For IL -1, receptors for S IL-6-R have not been detected. Conclusions: The chances of detecting arthropathy with type 1 diabetes with increasing TNF levels increase by 1.7 times, with an increase in IL-6 by 1.5 times. For type 2 diabetes, it is 1.8 and 1.3 times, respectively. Thus, TNF-α and IL-6 may be markers of the presence and progression of arthropathy in patients with diabetes mellitus.

PECULIARITIES OF PRO- AND ANTI-INFLAMMATION CYTOCINS LEVEL IN PREVENTION OF PROSTHETIC STOMATITIS IN PATIENTS WITH REMOVABLE ACRYLIC PROSTHESES

Due to the using of removable laminar dentures made of acrylic plastic, the complications in the form of chronic inflammation of the oral mucosa are developed in a certain category of patients, as a result of the complex impact of dentures on the tissues of the prosthetic bed in terms of mechanical, chemical, toxic, microbiological and immunological factors. In conditions of mechanical or chemical damage to the mucous membrane epithelium by the action of the microbial factor the activation of epithelial cells occurs and they acquire the properties of immunocompetent cells. As a result, the epithelial cells begin to produce cytokines. A special place in the implementation of acute inflammatory process is held by proinflammatory cytokines: interleukin (IL-1β) and tumor necrosis factor (TNF-α). They regulate all successive stages in the development of inflammation and an adequate response to the etiological factor. Interleukin 10 (IL-10) is the main anti-inflammatory cytokine, which inhibits the synthesis of proinflammatory cytokines. Determination of the level of pro- and anti-inflammatory cytokines is a diagnostic criterion in the development of prosthetic stomatitis and at the same time it is used to test the effectiveness of the proposed method of prevention or treatment of complications on the part of the tissues of the prosthetic bed.
 The aim of the research is to study the concentration of proinflammatory cytokines (TNFα, IL-1β) and anti-inflammatory cytokines (IL-10) in the oral fluid of patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic and dentures coated with fullerene C60 molecules.
 Material and methods of research. The study included 39 patients who were between 40 to 80 years old, including 19 male (48.7%) and 20 female (51.3%) subjects. The partial and complete defects of the dentition were diagnosed. The control group consisted of apparently healthy individuals, namely 10 people without defects of the dentition and periodontal tissue diseases.
 The patients were divided into 3 groups: the first group comprised apparently healthy people with intact dentition; the second group included patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic (20 patients) 7 days after prosthetics; the third group – patients with complete and partial defects of the dentition with removable laminar dentures made of acrylic plastic (20 patients) 30 days after prosthetics; the fourth group – patients with complete and partial defects of the dentition after prosthetics with removable laminar dentures made of acrylic plastic and coated with fullerene C60 molecules.
 The concentration of cytokines IL-1β and TNFα and IL-10 (pg / ml) was determined in the mouth before treatment at day 7, 2 weeks and 1 month. Statistical processing was conducted using STATISTICA 6.0 (StatSoft, USA).
 Results of the studies and their discussion. The results of the studies show that the level of cytokines in patients before prosthetics is higher than in apparently healthy people. On the 7th and 30th day after prosthetics in the study groups, there was a sharp increase in the concentration of proinflammatory and anti-inflammatory cytokines in the oral fluid as contrasted with the comparison group. These data confirm that activation of local synthesis of proinflammatory cytokines in the oral fluid is an important pathogenetic factor that determines the formation and maintenance of an active inflammatory response in the mucosa of the prosthetic bed. After fullerene C60 coating of the surface of denture, which is in contact with the tissues of the prosthetic bed, there is a significant decrease in the level of proinflammatory (IL-1β and TNFα) and anti-inflammatory (IL-10) cytokines.

Эффективность блокатора кальциевых каналов лерканидипина в качестве нефропротектора при лечении больных уролитиазом с обструктивным синдромом и мониторинг цитокинового профиля крови

to evaluate the nephroprotective effect of lercanidipine, its effect on the dynamics of creatinine clearance and blood cytokine levels in patients with nephrolithiasis with obstructive uropathy during renal drainage. 66 patients were included in the study with concretions of the pelvic segment and the presence of obstruction according to instrumental methods of examination. In order to prevent the occurrence of infectious complications before lithotripsy patients the first stage was performed installation of nephrostomic drainage, followed by antibacterial, anti-inflammatory therapy. Patients were divided into 2 groups: the first (33 patients) received standard therapy, the second (33 people) additionally received lercanidipine at a dose of 10 mg per day for 1 month. Determined the concentration of IL-8, VEGF, MCP-1, G-CSF and GM-CSF in the blood serum by the method of solid-phase ELISA. The glomerular filtration rate was calculated using the CKD-EPI formula. All studies were performed at the preoperative stage, on 7, 14, 21 and 28 days after renal drainage. In the appointment of lercanidipine, there was a more rapid decrease in levels of IL-8, VEGF, MS-1, GM-CSF in serum (21 days), and an improvement in renal function, compared with the group that did not receive nephroprotective therapy. The administration of lercanidipine may contribute to a more rapid recovery of renal function and normalization of blood cytokine levels. This drug can be used in the complex treatment of patients with nephrolithiasis with obstructive uropathy.

Long non-coding RNA zinc finger antisense 1 expression associates with increased disease risk, elevated disease severity and higher inflammatory cytokines levels in patients with lumbar disc degeneration.

This study aimed to investigate the correlation of long noncoding RNA zinc finger antisense 1 (lncRNA ZFAS1) expression with disease risk, disease severity and inflammatory cytokines levels in lumbar disc degeneration (LDD) patients.83 LDD patients underwent surgery and 28 traumatized, non-LDD patients underwent lumbar disc surgery (controls) were consecutively enrolled in this case-control study. Lumbar disc tissue was obtained during surgery and herniated nucleus pulposus (HNP) was isolated to detect lncRNA ZFAS1 expression and inflammatory cytokines mRNA levels by RT-qPCR, and determine protein levels of inflammatory cytokines by western blot.HNP lncRNA ZFAS1 expression in LDD patients was up-regulated compared with controls (P < .001), and receiver operating characteristic (ROC) curve showed lncRNA ZFAS1 expression disclosed a good predictive value for LDD risk with area under curve (AUC) 0.753 (95% CI 0.646–0.859). And after adjustment by age, gender and body mass index (BMI), lncRNA ZFAS1 (P = .017) remained to be an independent predictive factor for higher LDD risk. In addition, lncRNA ZFAS1 expression was positively associated with Modified Pfirrmann Grade (P = .015). As to inflammatory cytokines, lncRNA ZFAS1 expression was observed to be positively correlated with TNF-α (P = .002), IL-1β (P = .007) and IL-6 (P = .015) mRNAs expressions while reversely associated with IL-10 mRNA level (P = .014); and lncRNA ZFAS1 expression was also positively correlated with protein levels of TNF-α (P = .038) and IL-6 (P = .027) while reversely associated with IL-10 protein expression (P = .039).lncRNA ZFAS1 expression associates with increased risk, elevated disease severity and higher inflammatory cytokines levels in LDD patients.

P.274 Blood levels of cytokines in patients with type 1 bipolar disorder, in manic episode and after treatment response

Sleep apnea is the most pervasive sleep disorder as its severity causes even life-threatening health issues. The present study aimed to evaluate the self-administered questionnaire for assessing obstructive sleep apnea (OSA).The qualitative analysis compared and validated the Berlin (BQ), STOP-BANG (SBQ) and OSA50 questionnaires for sleep apnea assessment. In this preliminary study, we proposed four systematic analyses: gender, age group, smoking habit and working hour. One hundred and ninety participants were completed the screening test, where 52% of men and 48% of women with the mean age of 27 ± 10.In the present study, the results summarized that the male, young age adults, smoking person and long working hour's participants seem to be high prevalence of OSA. The statistical parameters such as sensitivity, specificity, positive predictive values (PPV) and negative predictive values (NPV) of BQ, SBQ and OSA50 questionnaires has been calculated. The OSA50 had the highest sensitivity (93%) when compared to BQ and SBQ having the sensitivity (91%). Significant difference (p < 0.05) in specificity was found in OSA50 (93%) when compared to the specificity of BQ and SBQ (90%). The PPV and NPV of BQ and SBQ questionnaires were 24% and 77% respectively. The OSA50 achieved 65% and 98%of PPV and NPV respectively.The study concluded that OSA50 questionnaire seems to be better validated OSA screening tool that can be used in resource-poor primary healthcare settings.

Effects of vitamin D supplementation on red cell indices and cytokines in patients with thalassemia: An open-label randomized clinical trial

Background and aim&#x0D; Deficiency of vitamin D is known as a health problem all over the world and a recognized clinical complication of beta thalassemia patients. Vitamin D acts as a hormone at the nuclear receptor rendering it a beneficial medication for a number of diseases. It is believed that vitamin D&#x0D; &#x0D; is important in the modulation of the inflammation system by regulating the formation of inflammatory cytokines and immune cells. This study aimed to investigate the effect of vitamin D supplementation on the red cell indices and cytokines levels in patients with beta thalassemia major, in an open label randomized clinical trial.&#x0D; Patients and Methods: this study performed an open-label randomized clinical trial in patients with beta thalassemia major. Forty-six patients completed the eight weeks clinical trial and were allocated to administer oral vitamin D3 supplement of 100,000 IU every two weeks as an add-on treatment. During the study, hematological indices, serum iron, ferritin, vitamin D, calcium and inflammatory markers (interleukin-6, interleukin-2 and interleukin-10) were evaluated before (at baseline) and after vitamin D supplementation for 8 weeks.&#x0D; Results: Vitamin D3 supplements significantly decreases interleukin-6 levels and elevates the serum levels of anti-inflammatory cytokines IL-2 and IL-10, it also significantly reduced serum ferritin level, but it did not alter the hematological indices.&#x0D; Conclusion: &#x0D; Our results suggest that administration of vitamin D has a potential anti-inflammatory role in beta thalassemia patients and reduces serum ferritin levels, which may reduce the burdens of iron overload in thalassemia patients.&#x0D;

Oligoclonal bands and levels of interleukin 4, interleukin 10, and tumor necrosis factor alpha in idiopathic intracranial hypertension Egyptian patients

BackgroundIdiopathic intracranial hypertension (IIH) is a neurological disorder of unknown pathophysiology with many proposed theories that involve CSF dynamics but recently, involvement of inflammatory and autoimmune processes has been postulated.ObjectivesTo investigate presence of oligoclonal bands (OCB) in cerebrospinal fluid (CSF) and serum cytokine level in patients with IIH.MethodsThis study was conducted on 27 IIH female patients and 21 age- and sex-matched control groups. Patient and control groups were subjected to measurement of interleukin-4 (IL-4), IL-10, and tumor necrosis factor α (TNF-α) levels in serum, and CSF oligoclonal bands was measured in the IIH patient group. Body mass index (BMI) was measured to both patients and control.ResultsSerum levels of IL-4, IL-10, and TNF alpha were significantly higher in IIH patients than controls (p < 0.001); 22% of IIH patients had positive OCB in CSF. There was a statistically significant difference regarding TNF-α level in OCB-positive and OCB-negative patients being higher in positive patients. No statistically significant correlation was found between serum levels of IL-4, IL-10, TNF-α, and BMI.ConclusionAutoimmune inflammatory process may play a role in pathophysiology of IIH.

Comprehensive treatment of rosacea patients taking into account the indicators of inflammatory mediators and the state of prooxidant-antioxidant homeostasis

Objective - increasing the effectiveness of treatment of patients with rosacea, taking into account changes in the indicators of inflammatory mediators, the state of proox­idant-antioxidant homeostasis and the clinical course of dermatosis by developing complex differentiated therapy using anti-inflammatory and antioxidant drugs.&#x0D; Material and methods. Under the supervision were 112 patients with rosacea (73 women, 39 men) aged 26 to 67 years. In patients with rosacea, the parameters of prooxidant­antioxidant homeostasis and inflammatory mediators (individual cytokines) were determined before and after standard treatment (comparative group) and the developed complex treatment using anti-inflammatory and antioxidant drugs (main group) according to known methods.&#x0D; Results. A significant increase in the activity of the processes of free radical oxidation of lipid and protein structures with the formation of oxidative stress and the state of endogenous intoxication was established in patients with rosacea, and their connection with the clinical course of dermatosis was proved. Significant growth in the serum of proinflammatory cytokines (interleukins - 8, 17A and 18) was investigated in patients with rosacea and their relationship with the clinical form of dermatosis was determined.The correlation between indicators of oxidative stress and the level of proinflammatory cytokines in patients with rosacea was found. An improved method of complex treatment of rosacea patients with combined and differentiated use of anti-inflammatory and antioxidant drugs has been developed.&#x0D; Conclusions. The developed complex treatment of rosacea patients with combined and differentiated use against the background of standard therapy of antihistamines with anti-inflammatory activity of fast (containing bilastine) and prolonged action (ketotifen fumarate) and antioxidant drug ethylmethylhydroxypyridine succinate significantly improves both the immediate and long-term clinical results of treatment of such patients.

Effects of vitamin D supplementation on red cell indices and cytokines in patients with thalassemia: An open-label randomized clinical trial

Background and aim Deficiency of vitamin D is known as a health problem all over the world and a recognized clinical complication of beta thalassemia patients. Vitamin D acts as a hormone at the nuclear receptor rendering it a beneficial medication for a number of diseases. It is believed that vitamin D is important in the modulation of the inflammation system by regulating the formation of inflammatory cytokines and immune cells. This study aimed to investigate the effect of vitamin D supplementation on the red cell indices and cytokines levels in patients with beta thalassemia major, in an open label randomized clinical trial. Patients and Methods: this study performed an open-label randomized clinical trial in patients with beta thalassemia major. Forty-six patients completed the eight weeks clinical trial and were allocated to administer oral vitamin D3 supplement of 100,000 IU every two weeks as an add-on treatment. During the study, hematological indices, serum iron, ferritin, vitamin D, calcium and inflammatory markers (interleukin-6, interleukin-2 and interleukin-10) were evaluated before (at baseline) and after vitamin D supplementation for 8 weeks. Results: Vitamin D3 supplements significantly decreases interleukin-6 levels and elevates the serum levels of anti-inflammatory cytokines IL-2 and IL-10, it also significantly reduced serum ferritin level, but it did not alter the hematological indices. Conclusion: Our results suggest that administration of vitamin D has a potential anti-inflammatory role in beta thalassemia patients and reduces serum ferritin levels, which may reduce the burdens of iron overload in thalassemia patients.

Plasma cytokine levels in patients with chronic alcohol overconsumption: Relations to gut microbiota markers and clinical correlates.

Alcohol-related morbidity may involve changes in the gut microbiota and immune dysregulation. We have previously demonstrated alterations in gut microbiota composition and functions in patients with alcohol overconsumption, and now aimed to investigate possible associations between cytokine levels, gut microbiota, and clinical symptoms. We included hospital inpatients with a history of chronic alcohol overconsumption. For comparison, we included control patients with a low alcohol intake. Cytokine levels (TGF-β1, TNF-α, IL-10, IL-8, IL-6, IFN-γ, MCP-1, IL-1RA, IL-1β, and IL-17) were determined using a customized V-plex assay. We then examined associations of cytokine levels with the abundance of Proteobacteria and Faecalibacterium, percentage of the short-chain fatty acid butyrate, psychiatric symptoms (Hospital Anxiety and Depression Scale), and biochemical liver variables. We included 28 patients with alcohol overconsumption (79% men), and 25 control patients (72% men). Patients with alcohol overconsumption had higher levels of IL-6 (p=0.002), IFN-γ (p=0.018), and MCP-1 (p=0.006), and lower levels of TGF-β1 (p=0.017) compared with control patients. Inverse correlations were found between Proteobacteria abundance and TNF-α (Rs=-0.55, p=0.02) and IL-8 (Rs=-0.58, p=0.014), and between Faecalibacterium and MCP-1 levels (Rs=-0.56, p=0.02) in the control patients, but not in patients with alcohol overconsumption. Patients with alcohol overconsumption reported more psychiatric symptoms, and these symptoms were inversely correlated with IL-10 levels. There were positive correlations between several of the assessed cytokines and biochemical liver variables, and negative correlations between cytokine levels and albumin. Patients with alcohol overconsumption had a cytokine profile suggestive of increased systemic inflammatory activity, with higher levels of pro-inflammatory cytokines (IL-6, IFN-γ, and MCP-1) and lower levels of anti-inflammatory cytokines (TGF-β1). The findings may represent a link between alcohol use and alcohol-related morbidity.

Determining of cytokine levels in the urine in clinical practice.

The paper presents data on the study of the content of cytokines (IL-1β, RAIL-1β, IL-2, IL-4, IL-10, IL-17A, TNF-, IFN-γ) in the morning urine using enzyme immunoassay in healthy individuals (n = 20) and in patients with acute glomerulonephritis (n = 93). The determination of cytokine levels in patients was carried out in the debut of the disease and 12 months after the onset of the disease. The obtained indicators of cytokine content in the urine are presented as absolute values in pg/ml and creatinine-normalized values calculated by the formula: cytokine level (pg/ml) / urine creatinine (µmol/ml). The study was made of changes in the content of cytokines in the urine of patients with glomerulonephritis with respect to a group of healthy individuals, as well as the dynamics of the content of cytokines in the urine during the 12-month observation period. The results of the study showed that the absolute values of cytokines in urine can distort the true picture of the cytokine profile of urine in renal pathology. Normalized values of the predominant number of pro- and anti-inflammatory cytokines (IL-1β, IL-2, IL-8, IL-10, IL-17A and TNF-α) in patients with glomerulonephritis were significantly higher than the corresponding indicators of healthy individuals. The normalized values of cytokines were shown to be as more sensitive indicators than absolute values in the course of analyzing differences in the cytokine profile in patients with glomerulonephritis, depending on chronic and acute course of the disease. These indicators influenced the outcome of glomerulonephritis, assessed, as a rule, 12 months after the onset of the disease. Thus, the low levels of IL-1β, IL-8 and IL-17А detected in the debut of the disease in combination with the high level of RAIL-1β determined the chronization of glomerulonephritis. So, the creatinine-normalized cytokine levels in the urine expand the possibilities of using the evaluation of the cytokine profile of urine to establish changes in the cytokine content in the urine in renal pathology and predict the chronization of glomerulonephritis.

Altered polyunsaturated fatty acid levels in relation to proinflammatory cytokines, fatty acid desaturase genotype, and diet in bipolar disorder

Inflammation and altered polyunsaturated fatty acid (PUFA) levels have been implicated in bipolar disorder (BD). A recent genome-wide association study identified a locus in the fatty acid desaturase (FADS) gene cluster conferring susceptibility to BD. In this study, we examined PUFA levels in patients with BD in relation to proinflammatory cytokines, FADS genotype, and dietary habits. We enrolled 83 patients with BD and 217 healthy controls who underwent plasma PUFA measurement. A subsample of 65 patients and 90 controls underwent plasma interleukin (IL)-6 and tumor necrosis factor alpha (TNFα) measurement, and three FADS single nucleotide polymorphisms (SNPs) were genotyped. Information on fish consumption was obtained by a self-reported diet history questionnaire. In comparing PUFA levels between patients and controls, significant differences were found for all 7 PUFAs tested. Specifically, n-3 eicosapentaenoic acid (EPA) level was decreased, and n-6 arachidonic acid level was increased in the patients (p < 0.0001 for both). Plasma IL-6 and TNFα levels were both significantly increased in the patients. Plasma EPA level was negatively correlated with IL-6 and TNFα levels. The FADS genotype, which was associated with increased n-6 PUFA levels, was also associated with marked elevation in TNFα levels. Less frequent fish intake was associated with low EPA and high IL-6 level. Taken together, our results provide strong evidence for altered plasma PUFA and proinflammatory cytokine levels in patients with BD. Furthermore, FADS genotype and fish consumption may contribute not only to altered PUFA levels but also to inflammation in BD.

The effect of minocycline on amelioration of cognitive deficits and pro-inflammatory cytokines levels in patients with schizophrenia

BackgroundCognitive deficits of schizophrenia are predictors of poor function, but antipsychotic medication has limited efficacy for cognitive deficits. These deficits in learning and memory may result from activity of pro-inflammatory cytokines, which microglia produce. The microglia inhibitor minocycline might arrest this cytokine damage to the hippocampus and reverse the cognitive deficits of schizophrenia. MethodsA double-blind, placebo-controlled study involved 75 patients with schizophrenia who randomly received low dose (100 mg/day) or high dose minocycline (200 mg/day) or placebo added to risperidone. MATRICS Consensus Cognitive Battery (MCCB) was used to assess the cognitive functioning, and serum levels of Interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor–α (TNF-α) were assessed. ResultsMinocyclinehigh dose group was significantly superior to minocyclinelow dose or placebo group not only for the improvements in cognitive tests' scores as well (P < 0.05), but for IL-1β and IL-6 serum levels reduction (P < 0.01). The amelioration of cognitive deficits with minocycline correlated not only with the remission of negative symptoms, but also with the reduction in serum levels of IL-1β and IL-6. ConclusionsMinocycline adjunctive treatment was effective in improving cognitive deficits of patients with schizophrenia. The beneficial effect of minocycline may be related to reducing pro-inflammatory cytokines through microglia inhibition.

Altered levels of inhibitory cytokines in patients with thalassemia major and gingival inflammation

Objective: To evaluate local and systemic levels of interleukin-10 (IL-10), IL-33, and tumor necrosis factor alpha (TNF-?) in Thalassemia major (TM) in the presence of gingival inflammation. Material and Methods: 58 patients (TM, n=29 and systemically healthy controls, n=29) were included to the study. IL-10, IL-33, and TNF-? levels were evaluated in gingival crevicular fluid (GCF), saliva and serum. Clinical periodontal measurements were recorded. Results: GCF IL-33 total amounts in TM and gingivitis group were elevated compared to systemically and periodontally healthy group (p=0.01). GCF IL-10, IL-33 and TNF-? concentrations were higher in TM and periodontally healthy group than the systemically healthy and gingivitis group (p=0.02, p=0.008, p=0.003). Serum IL-10 levels were elevated in TM and gingivitis compared to the systemically healthy and gingivitis (p=0.0009) and systemically and periodontally healthy (p=0.0007) groups. Serum IL-10 and TNF-? levels in TM and periodontally healthy group were higher than systemically and periodontally healthy group (p=0.01 and p=0.02). Conclusion: TM may potentially alter circulating levels of IL-33 and IL-10 and therefore, may affect the degree of periodontal inflammation locally or vice versa. Yet, the underlying mechanism linking the hematologic condition is not clear and deserves further investigation. KeywordsGingivitis; Thalassemia major; Interleukin-10; Interleukin-33; Tumor Necrosis Factor-alpha.

Evaluation of Cardiovascular Involvement and Cytokine Levels in Patients with Mucopolysaccharidosis

Evaluation of Cardiovascular Involvement and Cytokine Levels in Patients with Mucopolysaccharidosis

Comparison of the abundances of various bacterial Gneiss in gut microbiota between the highest and lowest levels of cytokines in patients with rheumatoid arthritis (RA). TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; IL-17A: interleukin-17A.

Comparison of the abundances of various bacterial Gneiss in gut microbiota between the highest and lowest levels of cytokines in patients with rheumatoid arthritis (RA). TNF-α: tumor necrosis factor-α; IL-6: interleukin-6; IL-17A: interleukin-17A.

562-P: The Effect of Vitamin D Supplementation on the Cytokines Levels in Patients with Type 2 Diabetes Mellitus and Diabetic Neuropathy

Background: Inflammation plays an important role in the pathogenesis of diabetic neuropathy (DN) in patients with type 2 diabetes (DM2). It seems that vitamin D ((VitD) could be involved in this process, however, the relationship between VitD status and inflammatory markers has not been wildly studied. Hypothesis: The aim was to study the association between VitD status and inflammatory markers in patients with DM2 and DN. Methods: The single-centre randomized study included 62 subjects with DM2 and DN (more than 4 points according to NDS), HbA1c up to 9%. Antihyperglycemic treatment was stable during the study. Patients were randomized in two groups: 5000 IU (group I) and 40000 IU (group II) of cholecalciferol supplementation per week. Body mass index (BMI), glycated hemoglobin (HbA1c), serum 25(OH)D level, Interleukins 6, 10 (IL-6, IL-10) were determined at baseline and after 24 weeks of treatment. The correlation analysis was evaluated by Pearson test. Results: Group I (n = 31, F16) and Group II (n = 31, F15) were matched for age, gender, BMI and baseline HbA1c levels. VitD insufficient/deficiency was revealed in 78% of diabetic patients with DN. After 6 months of VitD supplementation, the BMI, HbA1c and IL-6 levels significantly decreased, and 25(OH)D and IL-10 concentration increased only in Group II. We found associations between serum 25(OH)D and IL-6 (r = -0.912, p = 0.017) as well as IL-10 (r = 0.903, p = 0.014), and between HbA1c and IL-6 (r = 0.825, p = 0.031) and IL-10 (r = -0.897, p = 0.025) exclusively in Group II after treatment. Conclusions: VitD supplementation in 40000 IU of cholecalciferol per week during 6 months was associated with improved vitamin D status, pro-inflammatory markers and better glycemic control in patients with DM2 and DN. Vitamin D supplementation in 5000 IU per week did not change vitamin D status and inflammatory markers in this population. Disclosure A.P. Stepanova: None. T.L. Karonova: None. M. Galagoudza: None. E.Y. Vasileva: None. E.B. Jude: None.

Cytokine levels and their role in the etiopathogenesis of Burning Mouth Syndrome: A systematic review.

Burning Mouth Syndrome is characterized by variable symptoms that include pain, burning and paraguesia in an otherwise healthy-appearing oral mucosa. Although the etiopathogenesis of Burning Mouth Syndrome is unknown, some studies provide evidence of subclinical inflammation leading to disrupted cytokine levels. To investigate the expression of cytokines and role in the etiopathogenesis of Burning Mouth Syndrome. Online databases (MEDLINE and EMBASE) were searched from November 1986 to November 2018 for case control/cross-sectional studies comparing the levels of cytokines in patients with Burning Mouth Syndrome and healthy controls. A total of eight studies were included in the current review. Four studies were of high and four studies were of moderate quality. Seven studies evaluated IL-6, out of which four showed comparable results, two showed higher levels and one study reported lower levels in Burning Mouth Syndrome patients compared to controls. Four studies assessed IL-2, out of which two reported comparable results whereas one study reported higher levels and one study reported lower levels in Burning Mouth Syndrome patients compared to controls. IL-10 levels were measured in three studies that reported no significant differences in the levels between Burning Mouth Syndrome and healthy controls. The etiopathogenesis of Burning Mouth Syndrome is multifactorial. Studies have provided scientific evidence that inflammation plays a key role in Burning Mouth Syndrome pathogenesis. However, whether up-regulation or down-regulation of specific cytokines contribute to the etiopathogenesis of Burning Mouth Syndrome remains debatable. Further high-quality studies with larger sample size and assessing a wider array of cytokines are warranted in order to obtain strong conclusions.