Cynodon Transvaalensis Burtt-Davy Research Articles

Raising Mowing Height Improves Freeze Tolerance of Putting Green–type Bermudagrass

There is a growing trend of cultivating hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy] on golf course putting greens in the transition zone because of its excellent quality in the summer months, coupled with less pesticide input than creeping bentgrass (Agrostis stolonifera L.). However, the long-term success of bermudagrass putting greens is hindered by low temperatures in winter months, particularly in the transition zone. To address this issue, in addition to genetic improvement for cold hardiness through the development of new cultivars, effective management approaches are necessary to enhance the winter survival of putting green–type bermudagrass. The objective of this study was to investigate the relative freeze tolerance of four bermudagrasses and the effects of raising mowing height on the freeze tolerance of putting green–type bermudagrasses. In this study, two experimental putting green–type bermudagrasses (11X2 and OKC0805) along with cultivars TifEagle and OKC3920 were tested at two mowing heights (3.2 vs. 6.4 mm) at freeze temperatures that ranged between –4 and –11 °C. The lethal temperature to kill 50% of the population (LT50) as well as regrowth vigor during recovery were evaluated. Variety ‘OKC3920’ demonstrated enhanced freeze tolerance compared with ‘TifEagle’ at both mowing heights. Increasing the mowing height from 3.2 mm to 6.4 mm improved freeze tolerance for most genotypes tested in this study. After exposing the grasses to –8 °C for 1 hour, genotypes such as 11X2 exhibited better regrowth vigor and demonstrated a faster recovery. This study suggests that golf course managers can enhance winter resilience of bermudagrass putting greens by selecting genotypes strategically with superior freeze tolerance and raising mowing heights in the fall acclimation process.

The extended day length promotes earlier flowering of bermudagrass.

Day length is a very critical environmental factor affecting plant growth and development. The extension of light application time has been shown to promote flowering in the long-day plant and to shorten breeding time in some crops. However, previous research on the regulation of bermudagrass flowering by light application time is scarce. Therefore, this study investigated the effect of day length on the growth and flowering of bermudagrass by prolonging the light application time in a controlled greenhouse. Three different light application times were set up in the experiment: 22/2 h (22 hours light/2 hours dark), 18/6 h (18 hours light/6 hours dark), 14/10 h (14 hours light/10 hours dark). Results showed that extending the light application time not only promoted the growth of bermudagrass (plant height, fresh weight, dry weight) but also its nutrient uptake (nitrogen (N) and phosphorous (P) content). In addition, daily light integrals were different when flowering under different light application times. Most importantly, under the 22/2 h condition, flowering time was successfully reduced to 44 days for common bermudagrass (Cynodon dactylon [L.] pers) genotype A12359 and 36 days for African bermudagrass (Cynodon transvaalensis Burtt-Davy) genotype ABD11. This study demonstrated a successful method of bermudagrass flowering earlier than usual time by manipulating light application time which may provide useful insights for bermudagrass breeding.

Comparative analysis reveals chromosome number reductions in the evolution of African bermudagrass (Cynodon transvaalensis Burtt-Davy).

African bermudagrass (Cynodon transvaalensis Burtt-Davy) (2n=2x=18) belongs to the genus Cynodon, tribe Cynodonteae, subfamily Chloridoideae in the grass family Poaceae. The species is frequently crossed with common bermudagrass (Cynodon dactylon Pers.) in developing high-quality hybrid turf cultivars. Molecular resources for C. transvaalensis are scarce; thus, its genomic evolution is unknown. Recently, a linkage map consisting of 1278 markers provided a powerful tool for African bermudagrass genomic research. The objective of this study was to investigate chromosome number reduction events that resulted in the nine haploid chromosomes in this species. Tag sequences of mapped single nucleotide polymorphism markers in C. transvaalensis were compared against genome sequences of Oropetium thomaeum (L.f.) Trin. (2n=2x=20), a genomic model in the Cynodonteae tribe. The comparative genomic analyses revealed broad collinearity between the genomes of these two species. The analyses further revealed that two major interchromosomal rearrangements of the paleochromosome ρ12 (ρ1-ρ12-ρ1 and ρ6-ρ12-ρ6) resulted in nine chromosomes in the genome of C. transvaalensis. The findings provide novel information regarding the formation of the initial diploid species in the Cynodon genus.

Genetic and QTL mapping in African bermudagrass.

Cynodon transvaalensis Burtt-Davy is frequently used to cross with C. dactylon Pers. in the creation of F1 hybrid cultivars that are some of the most widely used in the worldwide turf industry. However, molecular resource development in this species is limited. Accordingly, the objectives of this study were to construct a high-density genetic map, and to identify genomic regions associated with establishment rate. In this study, we constructed the first high-density linkage map for African bermudagrass using a genotyping by sequencing approach based on 109 S1 progenies. A total of 1,246 single nucleotide polymorphisms and 32 simple sequence repeat markers were integrated in the linkage map. The total length of nine linkage groups was 882.3 cM, with an average distance of 0.69 cM per interval. Four genomic regions were identified to be associated with sod establishment rate. The results provide important genetic resources towards understanding the genome as well as marker-assisted selection for improving the establishment rate in bermudagrass breeding.

Goosegrass Control and Turfgrass Injury Following Metribuzin and Topramezone Application with Immediate Irrigation

Goosegrass (Eleusine indica L. Gaertn.) is a problematic C4 weedy grass species, occurring in the warmer regions of the world where it is difficult to selectively control without injuring the turfgrass. Furthermore, control efficacy is affected by plant maturity. End-user options for satisfactory goosegrass control has decreased; thus, the need for developing management techniques to improve the selectivity of POST goosegrass control options in turfgrass systems is ever increasing. One possible means of providing control, yet maintaining turf quality is immediately incorporating applied products via irrigation. Greenhouse and field trials were conducted in Pickens County, SC, with the objectives of 1) evaluating turfgrass injury following use of POST goosegrass control options; 2) assessing if irrigating (0.6 cm) immediately following the herbicide application reduces injury of ‘Tifway 419’ bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy]; and 3) determining if immediate irrigation influences goosegrass control at one- to three-tiller and mature growth stage. Following the application of herbicide treatments, irrigation was applied (+) or not applied (−). Treatments included the following: control (+/− irrigation); topramezone at 12.3 g a.i./ha (+/− irrigation); metribuzin at 420 g a.i./ha (+/− irrigation); and topramezone plus metribuzin (+/− irrigation) at 12.3 and 420 g a.i./ha. Irrigation treatment had minimum effect on greenhouse-grown goosegrass biomass, all treatments provided >85% control of 1- to 3-tiller goosegrass plants. However, control for mature plants was <50% for topramezone- and 60% to 70% for metribuzin-containing treatments. In field studies, at 1 week after treatment (WAT), the irrigated metribuzin and topramezone plus metribuzin had ≈37% and ≈16%, respectively, less goosegrass control vs. nonirrigated treatments. At 2WAT, irrigated metribuzin and irrigated topramezone plus metribuzin–treated plots, had ≈50% less mature goosegrass control vs. nonirrigated treatments. Irrigated herbicide treatments, however, experienced ≈23% less turfgrass injury at this time. At 4 WAT, irrigated metribuzin- and irrigated topramezone plus metribuzin–treated plots experienced reduced mature goosegrass control by ≈65% and ≈59%, respectively. Overall, incorporating POST herbicide applications via 0.6 cm of irrigation reduced turfgrass injury by at least 20% for all herbicide treatments, while maintaining goosegrass control.

Comparing evapotranspiration rates of tall fescue and bermudagrass in North Carolina

Increasing water conservation efforts across landscapes necessitate the establishment of turfgrasses that require less water to sustain functionality. Therefore, it is important to consider concurrent water use potential of popular grass species such as tall fescue (Festuca arundinacea Schreb.) and hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy]. The primary objective of this field study was to compare water use characteristics of ‘TifTuf’ a recently released bermudagrass cultivar with reported drought tolerance with a commonly planted tall fescue/bluegrass mix in North Carolina. A secondary objective was to quantify minimum irrigation requirements during establishment from sod when planted during spring and summer. Direct measurements of actual evapotranspiration (ETa) were made through weighing of non-water stressed lysimeters planted with ‘Triple Threat’ tall fescue and TifTuf hybrid bermudagrass. Actual evapotranspiration rates during the first 14 days after planting (DAP) were 3.3 and 4.3 mm d−1 for bermudagrass and tall fescue, respectively, averaged across spring plantings. Tall fescue ETa 14 DAP was no different during summer establishment, while bermudagrass ETa increased to 4.3 mm d−1. After 14 DAP, cumulative bermudagrass ETa was 44% less than tall fescue when established in spring. Cumulative bermudagrass ETa was similar to tall fescue (3% less) following summer establishment. Both grasses provided acceptable turf quality (TQ ≥ 6), when planted during spring, unlike tall fescue which resulted in unacceptable TQ following summer establishment. Results indicate the use of TifTuf bermudagrass can provide acceptable quality in the landscape while significantly reducing turfgrass water use compared to Triple Threat tall fescue when adapted to localized conditions.

Immediate Irrigation Improves Turfgrass Safety to Postemergence Herbicides

Summer annual grassy weeds such as goosegrass (Eleusine indica L. Gaertn.) continue to be problematic to control selectively with postemergence (POST) herbicides within turfgrass stands. In recent years, reduced performance by certain herbicides (e.g., foramsulfuron), cancellation of goosegrass-specific herbicides (e.g., diclofop-methyl), and cancellation and/or severe use reductions of other herbicides [e.g., monosodium methanearsonate (MSMA)] have limited the options for satisfactory control and maintenance of an acceptable (≤30% visual turfgrass injury) turfgrass quality. Currently available herbicides (e.g., topramezone and metribuzin) with goosegrass activity typically injure warm-season turfgrass species. The objectives of this research were to evaluate both ‘Tifway 419’ bermudagrass [Cynodon dactylon (L.) Pers. ×Cynodon transvaalensis Burtt-Davy] injury after treatment with POST herbicides, and to determine whether irrigating immediately after application reduces turfgrass injury. Treatments were control (± irrigation); topramezone (Pylex 2.8C; ± irrigation); carfentrazone + 2,4-D + dicamba + 2-(2-methyl-4-chlorophenoxy) propionic acid (MCPP) (Speedzone 2.2L; ± irrigation); carfentrazone + 2,4-D + dicamba + MCPP in combination with topramezone (± irrigation); metribuzin (Sencor 75DF; ± irrigation); mesotrione (Tenacity 4L; ± irrigation); simazine 4L (±irrigation); and mesotrione + simazine (± irrigation). Irrigated treatments were applied immediately with a hand hose precalibrated to apply 0.6 cm or 0.25 inch (≈6.3 L). Visual turfgrass injury for combined herbicide treatments for the irrigated plots was 6% 4 days after treatment (DAT), 12% 1 week after treatment (WAT), 17% 2 WAT, and 6% 4 WAT, whereas nonirrigated plots had turfgrass injury of 14% at 4 DAT, 31% 1 WAT, 35% 2 WAT, and 12% 4 WAT. Irrigated pots had normalized differences vegetative indices (NDVI) ratings of 0.769 at 4 DAT, 0.644 at 1 WAT, 0.612 at 2 WAT, and 0.621 at 4 WAT, whereas nonirrigated plots had the lowest (least green) turfgrass NDVI ratings of 0.734 at 4 DAT, 0.599 at 1 WAT, 0.528 at 2 WAT, and 0.596 at 4 WAT. These experiments suggest turfgrass injury could be alleviated by immediately incorporating herbicides through irrigation.

Leaf Wetness Influences Turf Colorant Application

Core Ideas Conditions during application can impact the visual quality of turf colorants. The response of measured colorant color parameters to pre-application irrigation was variable among products tested. Turf colorants with increased product viscosity benefit from pre-application irrigation. Turf colorants are primarily used on warm-season grasses throughout the southern United States to maintain aesthetic quality leading into and during winter dormancy. To maximize aesthetic quality, turf colorants must be applied when certain conditions are present. The objective of this field study was to evaluate the presence of leaf moisture during turf colorant application to dormant turfgrass and its effect on measured color parameters (color, intensity, and hue angle). Four turf colorants were applied to wet and dry (i.e., irrigated and non-irrigated) dormant ‘Tifsport’ hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy] and ‘El Toro’ zoysiagrass (Zoysia japonica Steud.). Color parameters were evaluated weekly for 5 consecutive weeks following turf colorant application. Average colorant color and intensity ratings were 14 and 10% higher when dormant turfgrass was irrigated prior to colorant application, respectively. Color and intensity ratings were unaffected by leaf wetness following applications of Endurant Premium and Green Turf Paint applications. However, color and intensity ratings increased by 38 and 26%, respectively, when turfgrass was irrigated prior to the application of Green Lawnger and Ultradwarf Super. Negative effects (i.e., streaking) commonly seen during application of turf colorants higher in viscosity were alleviated as a result of pre-application irrigation. Hue angles quantified from digital images were unaffected by the presence of leaf wetness during colorant application. Results from this research indicate turfgrass managers should consider wetting dormant turfgrass through a quick irrigation cycle to mitigate possible unattractive streaking that result from the use of higher-viscosity products or increased application rates.

Transcriptional regulation of heat shock proteins and ascorbate peroxidase by CtHsfA2b from African bermudagrass conferring heat tolerance in Arabidopsis.

Heat stress transcription factor A2s (HsfA2s) are key regulators in plant response to high temperature. Our objectives were to isolate an HsfA2 gene (CtHsfA2b) from a warm-season grass species, African bermudagrass (Cynodon transvaalensis Burtt-Davy), and to determine the physiological functions and transcriptional regulation of HsfA2 for improving heat tolerance. Gene expression analysis revealed that CtHsfA2b was heat-inducible and exhibited rapid response to increasing temperature. Ectopic expression of CtHsfA2b improved heat tolerance in Arabidopsis and restored heat-sensitive defects of Arabidopsis hsfa2 mutant, which was demonstrated by higher survival rate and photosynthetic parameters, and lower electrolyte leakage in transgenic plants compared to the WT or hsfa2 mutant. CtHsfA2b transgenic plants showed elevated transcriptional regulation of several downstream genes, including those encoding ascorbate peroxidase (AtApx2) and heat shock proteins [AtHsp18.1-CI, AtHsp22.0-ER, AtHsp25.3-P and AtHsp26.5-P(r), AtHsp70b and AtHsp101-3]. CtHsfA2b was found to bind to the heat shock element (HSE) on the promoter of AtApx2 and enhanced transcriptional activity of AtApx2. These results suggested that CtHsfA2b could play positive roles in heat protection by up-regulating antioxidant defense and chaperoning mechanisms. CtHsfA2b has the potential to be used as a candidate gene to genetically modify cool-season species for improving heat tolerance.

Performance of Winter Overseeded and Colorant-treated ‘Tifway’ Bermudagrass Receiving Weekly Irrigation and Simulated Traffic

Municipal water restrictions across the southern and southwestern United States have created additional challenges for maintaining safe playing surfaces on recreational turf facilities. In recent years, many cities within these regions have begun to impose irrigation restrictions during winter months. Although winter overseeding has been regularly practiced in these areas, interest and use of colorants as an alternative to overseeding has grown due to decreasing water availability and budget concerns. Data on relative performance of colorant-treated vs. overseeded dormant turf would be of interest to turf managers, because colorants may be more cost-effective and require less water than winter overseeding. The objectives of this 2-year field study were to evaluate effects of winter treatments on performance (green cover, surface hardness, and soil moisture attributes), turfgrass injury resulting from simulated traffic, and spring transition of ‘Tifway’ bermudagrass (Cynodon dactylon L. × Cynodon transvaalensis Burtt-Davy) under a 1-day per week irrigation schedule. Treatments included 1) untreated bermudagrass, 2) fall colorant-treated bermudagrass, 3) perennial ryegrass (Lolium perenne L.) overseeded bermudagrass, and 4) turf-type annual ryegrass (Lolium multiflorum Lam.) overseeded bermudagrass. In both years, treatment differences were detected for percent green cover, soil volumetric water content (VWC), percent visual turfgrass injury, surface hardness, and percent bermudagrass transition. Percent green cover and visual turfgrass injury levels were similar between annual and perennial ryegrass in year 1, whereas loss of green cover and greater turfgrass injury were noted in annual ryegrass during the spring of year 2. Residual benefits of fall colorant applications extended into February of year 1, but dissipated by late December of year 2, likely due to higher rainfall and warmer temperatures, which prevented full bermudagrass shoot dormancy. Overseeding reduced bermudagrass spring transition by up to 50% compared with untreated and colorant-treated plots. Fall colorant treatments did not accelerate bermudagrass transition compared with untreated plots. Results of the study demonstrate that environmental differences from season to season can impact the relative benefits derived from colorant applications, as well as the performance of annual and perennial ryegrass.

Transpiration responses of warm-season turfgrass in relation to progressive soil drying

Developing turfgrass with good drought resistant is a major goal in breeding programs when water scarcity is one of the long-term challenges facing the turfgrass industry. It has been shown that plant transpiration does not decline until available soil water drops below a certain threshold. Studying the species and genotypic differences in this threshold may lead to turfgrass that conserve water and retain turfgrass quality during drought. The objective of this study was to characterize and compare the transpiration response of 19 warm-season turfgrass genotypes and cultivars in five species during soil drying and well-watered conditions in the greenhouse. The species included in the study were: Zoysia japonica (Steud), Zoysia matrella L., common bermudagrass (Cynodon dactylon L. Pers. var. dactylon), African bermudagrass (Cynodon transvaalensis Burtt-Davy), hybrid bermudagrass (C. dactylon L. var. dactylon×C. translvaalensis Burtt-Davy) and St. Augustinegrass [Stenotaphrum secundatum (Walter) Kuntze]. They were evaluated for evapotranspiration rate (ET) and the sensitivity of transpiration to soil drying which was indicated by a break point of the fraction transpirable soil water (FTSW). The range of break point during dry down was from 0.25 to 0.41, and genotypes within species had different ET when well-watered (ETck). Break point values were not correlated with the number of days to the endpoint, where ET declined below 10% of the control. Instead, the number of days to endpoint was negatively correlated with ETck (−0.59, P≤0.01). Thus, these results found significant variability in turfgrass break point and ETck that could contribute to water conservation, and a better understanding of drought responses in warm season turfgrasses.

Root Development and Profile Characteristics of Bermudagrass and Zoysiagrass

Irrigation for commercial and residential turf is becoming limiting, and water scarcity is one of the long-term challenges facing the turfgrass industry. Potential root development and profile characteristics of turfgrass provide important information regarding their drought resistance mechanisms and developing drought-resistant cultivars. The objective of this study was to determine the potential root development and root profile characteristics of two bermudagrass species and two zoysiagrass species using experimental lines and commercial cultivars. The species evaluated in the study were: African bermudagrass (Cynodon transvaalensis Burtt-Davy), common bermudagrass (CB) [Cynodon dactylon (L.) Pers. var. dactylon], Zoysia japonica (ZJ) (Steud), and Zoysia matrella (ZM) L. Plants were grown outdoors in clear acrylic tubes encased in poly vinyl chloride (PVC) sleeves. The experimental design was randomized complete block design with four replications. Rates of root depth development (RRDD) during the first 30 days were obtained. Root length density (RLD) in four different horizons (0–30, 30–60, 60–90, and 90–120 cm) was determined 60 days after planting. Specific root length (SRL, m·g−1) was also calculated dividing total root length by total root dry weight (RDW). The root depth in four turfgrass species increased linearly during the first 30 days after planting. Common bermudagrass (CB) had high RRDD and uniform RLD in different horizons, while ZM accumulated the majority of its roots in the upper 30 cm. Z. matrella had higher RLD than CB in the upper 30 cm. African bermudagrass had higher SRL than CB. There was limited variation within the two African bermudagrass genotypes studied except at the lowest horizon (90–120 cm). Two genotypes in CB and ZJ, respectively, including ‘UF182’ (ZJ), which consistently ranked in the top statistical group for RRDD, and RLD for every horizon, and ‘UFCD347’ (CB) demonstrated greater RLDs in the lower horizons in comparison with the commercial cultivars.

Consumptive water use and crop coefficients for warm-season turfgrass species in the Southeastern United States

Increased urban demand for landscape irrigation, as well as interest in promoting water-use efficient species by municipalities, water purveyors, and homeowners associations emphasize the need for comparative data on consumptive water use by warm-season lawn grasses. The objective of this study was to quantify actual evapotranspiration (ETa) and to develop crop coefficients (Kc) for four warm-season turfgrass species, namely ‘Tifway’ bermudagrass (Cynodon dactylon (L.) Pers. x Cynodon transvaalensis Burtt-Davy), ‘Empire’ zoysiagrass (Zoysia japonica Steud.), ‘Floratam’ St. Augustinegrass [Stenotaphrum secundatum (Walter) Kuntze], and ‘Argentine’ bahiagrass (Paspalum notatum Flugge). Crop coefficients were derived by dividing ETa (measured directly from lysimeter weight change over 24 to 72-h periods) by reference evapotranspiration (ETo) calculated from the ASCE–EWRI Standardized Method using onsite weather station data. Data were collected over three seasons from non-stressed, well-watered turf. For 17 of the 30 measurement periods, Kc did not differ among the 4 species, and on 24 of 30 periods zoysiagrass, bermudagrass, and St. Augustinegrass Kc did not differ from one another. A trend toward elevated Kc was observed in bahiagrass in years 2 and 3, particularly during early spring measurement periods. Kc values for all species fluctuated across seasons and years, peaking to ∼0.8 during active growth periods when vapor pressure deficit and solar radiation were greatest, and declining to ∼0.3 in late fall and winter. Root growth differences among the species appeared to have a stronger relationship to ET rates than did shoot growth rate. Results demonstrated that the commonly recommended warm-season turf coefficient of 0.6, while approximating overall average annual ETa, under-predicted ETa during active growth periods and over-predicted ETa during late fall and winter periods, when turf was slowly growing or quiescent. The results indicate seasonal refinement of Kc values may be needed to more effectively meet consumptive water use requirements of warm-season turfgrasses.

Development and characterization of genomic SSR markers in Cynodon transvaalensis Burtt-Davy

Simple sequence repeat (SSR) markers are a major molecular tool for genetic and genomic research that have been extensively developed and used in major crops. However, few are available in African bermudagrass (Cynodon transvaalensis Burtt-Davy), an economically important warm-season turfgrass species. African bermudagrass is mainly used for hybridizations with common bermudagrass [C. dactylon var. dactylon (L.) Pers.] in the development of superior interspecific hybrid turfgrass cultivars. Accordingly, the major objective of this study was to develop and characterize a large set of SSR markers. Genomic DNA of C. transvaalensis '4200TN 24-2' from an Oklahoma State University (OSU) turf nursery was extracted for construction of four SSR genomic libraries enriched with [CA](n), [GA](n), [AAG](n), and [AAT](n) as core repeat motifs. A total of 3,064 clones were sequenced at the OSU core facility. The sequences were categorized into singletons and contiguous sequences to exclude redundancy. From the two sequence categories, 1,795 SSR loci were identified. After excluding duplicate SSRs by comparison with previously developed SSR markers using a nucleotide basic local alignment tool, 1,426 unique primer pairs (PPs) were designed. Out of the 1,426 designed PPs, 981 (68.8 %) amplified alleles of the expected size in the donor DNA. Polymorphisms of the SSR PPs tested in eight C. transvaalensis plants were 93 % polymorphic with 544 markers effective in all genotypes. Inheritance of the SSRs was examined in six F(1) progeny of African parents 'T577' × 'Uganda', indicating 917 markers amplified heritable alleles. The SSR markers developed in the study are the first large set of co-dominant markers in African bermudagrass and should be highly valuable for molecular and traditional breeding research.

White Clover (Trifolium repens) Establishment within Dormant Bermudagrass Turf: Cultural Considerations, Establishment Timing, Seeding Rate, and Cool-season Companion Grass Species

White clover (Trifolium repens L.) inclusion is a proposed means of increasing the sustainability of certain low-maintenance turfgrass scenarios through increased pollinator habitat and as a result of the legume’s ability to biologically fix atmospheric nitrogen (N). Proper white clover establishment is key to maximizing stand uniformity and N contribution to associated grasses. However, there are few guidelines for white clover establishment within warm-season turfgrasses. Four studies were conducted to evaluate seeded white clover establishment within a dormant hybrid bermudagrass [Cynodon transvaalensis Burtt-Davy × C. dactylon (L.) Pers.] lawn as affected by 1) pre-seeding mechanical surface disruption; 2) establishment timing; 3) seeding rate; and 4) companion grass species. White clover establishment was improved by scalping before October seeding, but these effects were not further enhanced by the addition of verticutting or hollow tine aerification. Unscalped turfgrass yielded nearly 50% lower white clover densities than those scalped before seeding, possibly as a result of decreased seed-to-soil contact and increased bermudagrass competition. January and February establishment dates generally yielded the lowest spring clover densities, whereas October timing yielded superior establishment. Clover densities resulting from six seeding rates (0, 0.4, 0.8, 1.5, 3.0, and 6.0 g live seed/m2) were fit to the linear model (y = y0 + axb, where y equals trifoliate leaves/m2 and x is equal to initial seeding rate). An important feature of this model was that it accurately represented the diminishing response of increasing seeding rate. Clover establishment was negatively correlated with companion grass densities with the largest densities occurring when planted with tall fescue and the smallest when planted with annual ryegrass. Ultimately, scalping alone or in combination with other mechanical surface disruption should be paired with a clover variety acceptable to the height of cut and the environmental conditions of individual scenarios. Likewise, seeding rates and the decision to include a cool-season companion grass species will be dependent on the use of a turf and the desired green cover.

Generalist Predators and Predation of Black CutwormAgrotis ipsilonLarvae in Close Mown Creeping Bentgrass

Predators play a significant role in regulating pest outbreaks in turfgrass (Potter 2001). The most common predaceous arthropods recorded from turfgrass are spiders, predatory beetles (Carabidae and Staphylinidae) (Kunkel et al. 1999; Rothwell & Smitley 1999), predatory hemipterans, and ants (Lopez & Potter 2003; Frank & Shrewsbury 2004a). Natural enemies and their effects can be monitored by pitfall traps (e.g., Kunkel et al. 1999; Frank & Shrewsbury 2004a) or by exposing prey overnight without direct observation (Kunkel et al. 1999; Lopez & Potter 2000; Frank & Shrewsbury 2004a). Furthermore, most studies are conducted in roughs or fairways, which have more biodiversity (Lopez & Potter 2000), greater mowing height, and are less intensely managed than greens. Predation and predator abundance can be significantly different based on mowing heights (Rothwell & Smitley 1999). A better understanding of ecosystem services in putting greens may provide insights into reducing pesticide inputs. The objectives of this study were to determine the types of nocturnal predators in putting greens and determine which taxa are involved in predation of black cutworm larvae (Agrotis ipsilon Hufnagel) in close-mown bentgrass. Black cutworms provide a model system to investigate predation in turfgrass. Black cutworm larvae feed nocturnally on creeping bentgrass ( Agrostis stolonifera L.) greens causing pock marks that interfere with ball roll and aesthetics (Potter 1998). Predation trials were conducted at the Turfgrass Research Unit, Auburn, Alabama and the Grand National Golf Course, Opelika, Alabama. Plots or greens had not been sprayed with insecticides for at least 2 wk prior to each experiment. At the Turfgrass Research Unit, 2 plots of creeping bentgrass were used. A 706 m2 plot was used for larval predation trials, and a 929 m2 plot for predator surveys. Turfgrass in these plots was maintained at 0.33 cm with surrounding turf 2.54 cm high. Putting greens have 'Dominant' creeping bentgrass, whereas 'El Toro* zoysiagrass ( Zoysia japonica Steud), and 'Tifway' bermudagrass (Cynodon dactylon L. Pers Cynodon transvaalensis Burtt-Davy) were established in collars. Greens and collars are maintained at 0.36 and 1.27 cm, respectively. Larvae were obtained from a commercial in-

Growth and Evapotranspiration Response of Two Turfgrass Species to Nitrogen and Trinexapac-ethyl

Resolution of the effects of trinexapac-ethyl and nitrogen (N) application rate on evapotranspiration of low-cut, prostrate turfgrass species such as creeping bentgrass (Agrostis stolonifera L.) and hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt-Davy] has not been explored. This study sought to examine the integrated growth and evapotranspiration responses of these two turfgrass species to two N rates and application of trinexapac-ethyl, a commonly used growth-regulating compound for suppressing turfgrass shoot growth. ‘Penncross’ creeping bentgrass and ‘Tifdwarf’ bermudagrass were studied. We hypothesized that application of trinexapac-ethyl and/or lower N application rates might result in lower rates of turfgrass evapotranspiration. Two greenhouse studies were conducted over 6-week periods in 2008. A completely randomized design was used to support a two species × two N rate × two trinexapac-ethyl rate factorial. Shoot and root growth and evapotranspiration were determined from the two grass species when maintained in well-watered conditions and grown in pots. The treatments were either a high N (1.2 g N/m2/week) or a low N (0.3 g N/m2/week) application and either 0 or 28 mg·m−2 a.i. trinexapac-ethyl. Application of trinexapac-ethyl and use of the low N rate each significantly reduced shoot growth in both species; however, neither factor caused a reduction in turfgrass water use. The high N rate promoted increased root growth in both species during the spring, but there was no effect of either factor on rooting during the summer study. These results indicate that although decreasing N application rates and applying trinexapac-ethyl effectively suppress shoot growth, neither is likely to have any impact on overall water requirements for these species when maintained in well-watered conditions.

Soil and Effluent Irrigation Nutrient Monitoring of an Alabama Golf Course

Although wastewater (effluent) application to golf courses is a practice of interest across the USA, research has largely centered on effluent use in the arid southwest. The objective of this research project was to sample effluent used to irrigate a southeastern golf course, and to also sample soil receiving effluent application. From October 2006 to October 2008 soil and effluent samples were taken monthly from three replicate fairways, the sewage lagoon itself, and a “no effluent” (control) area. Both soil and effluent samples were analyzed for nitrate (NO3-N), ammonium (NH4-N), phosphate (PO4-P), and electrical conductivity (EC). Average EC of effluent was low (0.23 dS/m), and average EC in effluent treated soil was 0.26 dS/m, as compared to 0.21 dS/m in soil not receiving effluent. Extractable soil PO4-P ranged from a low of 1.6 to a high of 62.2 ug/g, and always remained within the “medium” range for a P soil test, indicating that P was not accumulating in the upper 7.6 cm of soil. Highest and lowest effluent NO3-N was 7.24 and 0.12 ug/mL, respectively, with a 2-year average of 1.88 and 2.11 ug/mL measured in fairway irrigation and lagoon effluent, respectively. In this 2-year period, continuous irrigation with effluent water posed no observed environmental or agronomic hazards to the sampled 7.6-cm deep soil layer nor to the hybrid bermudagrass [Cynodon dactylon (L.) Pers. X Cynodon transvaalensis Burtt-Davy] on the golf course fairways.

Growth Habit Determination of Genotypes of African Bermudagrass

Six African bermudagrass (Cynodon transvaalensis Burtt-Davy) genotypes, one common bermudagrass [C. dactylon (L.) Pers. var. dactylon] genotype, and ‘Tifway’ (C. dactylon × transvaalensis) hybrid bermudagrass were evaluated for shoot type, leaf angle, and shoot angle. Evaluations were conducted to determine if these measurements could be used to differentiate among upright, intermediate, and prostrate growth habits. Significant differences were found for all three techniques, but attempts to group plants together as having prostrate, intermediate, or upright growth habits was not possible. ‘Tifway’ was intermediate between the African bermudagrass genotypes and the common genotype for shoot type observations, but was more similar to upright-growing African bermudagrass for leaf angle and the more prostrate-growing common bermudagrass for shoot angle. Quantification of shoot type and leaf angle did not appear as useful as shoot angle measurements for screening germplasm to identify upright or prostrate growth habits in bermudagrass.

Physiological acclimation of seashore paspalum and bermudagrass to low light

Physiological responses play an important role in low light tolerance in plants. The acclimations of photosynthetic activities, total soluble protein, and antioxidant enzyme were characterized in low light-tolerant ‘Sea Isle 1’ seashore paspalum ( Paspalum vaginatum Swartz) and intolerant ‘TifSport’ hybrid bermudagrass ( Cynodon dactylon L. × Cynodon transvaalensis Burtt-Davy) grown under high light control (HL, 500–900 μmol m −2 s −1), low light (LL, 60–100 μmol m −2 s −1), and LL to HL transfer in the greenhouse. Shade cloth was used to create the LL treatment. Sea Isle 1 maintained higher turf quality and normalized difference vegetation index (NDVI) than that of TifSport under LL. Chlorophyll a (Chl a) and chlorophyll b (Chl b) content were reduced by 34–36% in Sea Isle 1 and by 51–63% in TifSport at day 35 of LL, respectively, relative to their HL levels. Chlorophyll a/b ratio (Chl a/b) generally was not affected by LL for Sea Isle 1, but the values under LL increased to above HL level in TifSport. Photochemical efficiency (Fv/Fm) remained constant under LL in both species. Total soluble protein content (SPC), water-soluble carbohydrate content (WSC), catalase (CAT) activity, and ascorbate peroxidase (APX) activity decreased 22% and 55%, 52% and 66%, 68% and 77%, and 40% and 66% under LL for Sea Isle 1 and TifSport, respectively. After grasses were transferred from LL to HL, Chl a, Chl b, SPC, WSC, and CAT activity increased, but to a higher extent in Sea Isle 1. A short-term reduction in Fv/Fm was exhibited in both species after light transfer but a further decrease was found in TifSport. The results indicated that the relative higher levels of Chl, SPC, WSC, and antioxidant enzyme activities under LL contributed to better LL tolerance in grass species. More rapid recovery of these parameters from LL to HL was a characteristic of the tolerant Sea Isle 1 seashore paspalum relative to the intolerant TifSport bermudagrass.