Cyclic Voltammetric Studies Research Articles

Electrochemical oxidation of haematoxylin at poly(alanine) modified carbon paste electrode: A cyclic voltammetric study

Electrochemical oxidation of haematoxylin (HX), a bioactive bis-catechol molecule was studied at poly(alanine) modified carbon paste electrode (CPE) by using cyclic voltammetric technique followed by its electrochemical characterisation. The poly(alanine) modified CPE exhibited a high sensitivity towards the oxidation of HX. The effect of scan rate was found to be adsorption-controlled. The concentration study in the range of 20–160μM was calibrated and the limit of detection (3S/M) and limit of quantification (10S/M) were calculated to be 0.102μM and 0.34μM respectively. Because of the high sensitivity of the fabricated poly(alanine) modified CPE it can be used as a promising electrochemical sensor for the determination of HX.

Synthesis and characterization of biopolymer based hybrid hydrogel nanocomposite and study of their electrochemical efficacy

A highly competent material, based on poly lactic acid (PLLA) grafted hydroxypropyl guar gum (HPG-g-PLLA) and polypyrrole/carboxylated multiwalled carbon nanotube (PPy/C-MWCNT) composite of various binary composition and copolymer of one of these nanocomposites have been synthesized successfully by in-situ polymerization. The environmentally affable nanocomposites have been characterized by spectroscopy, microscopy and thermogravimetry. Cytotoxicity of bio-nanocomposite has been inquired by cell viability study, which reveals its eco-friendly nature. The electrochemical properties of the biomaterials have been appraised by cyclic voltammetric studies. The PPy/C-MWCNT composite having 1 wt% C-MWCNT appears as the optimum composition from electrochemical studies. The hydrogel nanocomposite (HPG-g-PLLA5/0.5) copolymer behaves as a super ordinate material than pure PPy and PPy/C-MWCNT in every aspect of electrochemical properties like current density, stability, processibility and reversibility. Moreover the hydrogel nanocomposite, making electrode fabrication more simple and binder-free, nullifies all the interfacial complications arising from binders as well.

Fabrication of PEI-Fc/SGO multilayer films modified electrode through LBL assembly technique for the detection of dopamine

In this work, a sensor for the detection of dopamine (DA) based on electroactive polymer (PEI-Fc)/sulfonic acid-functionalized graphene oxide (SGO) multilayer film-modified glassy carbon electrode (GCE) through layer-by-layer (LBL) method was fabricated. Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy revealed the successful functionalization of GO into SGO with negatively charged sulfonic groups. The obtained PEI-Fc/SGO multilayer films were characterized using scanning electron microscopy (SEM) and ultravioletvisible spectrometer (UV-Vis). Cyclic voltammetric studies demonstrated that the modified electrode presented enhanced electrocatalytic performance towards DA in terms of peak currents compared with bare GCE. The choronoamperometric results indicated that the modified electrode exhibited a wide linear range from 1 to 695 μM with a low detection limit of 0.255 μM towards the detection of DA. Besides, the modified electrode exhibited good selectivity, excellent reproducibility, and high stability of at least 1 month.

Carbon black nanospheres modified with Cu (II)-phthalocyanine for electrochemical determination of Trimethoprim antibiotic

This work investigates the electrochemical sensing properties of glassy carbon (GC) electrodes modified with Printex L6 carbon black (PC) and copper (II) phthalocyanine (CuPh) films. Successful incorporation of the CuPh into carbon black was confirmed by FEG-SEM, EDS and TEM analysis. The transition from nanocrystalline graphite to amorphous carbon material could be evidenced for Printex L6 carbon black using Raman analysis. The modified material (CuPh/PC/GC) demonstrated electrocatalytic effect towards TMP detection according to cyclic voltammetric studies. CuPh modification resulted in strong adsorption to carbon black and TMP due to π-π interactions. Scan rate studies proved reactions across the electrode/electrolyte interface progressively change from adsorption to diffusion. The developed method presented linear relationship in the ranges of 0.4–1.1 µmol L−1 and 1.5–6.0 µmol L−1 with limit of detection of 0.6 µmol L−1 under optimized experimental conditions. The proposed CuPh/PC/GC electrode was effectively employed towards the detection of TMP in natural water samples.

Sonochemically recovered silver oxide nanoparticles from the wastewater of photo film processing units as an electrode material for supercapacitor and sensing of 2, 4, 6-trichlorophenol in agricultural soil samples

The present work describes the sensing application and supercapacitive behavior of silver oxide nanoparticles recovered from wastewater of photo film processing units via one-pot green sonochemical recovery process. The recovered silver oxide nanoparticles (Ag2O NPs) were characterized by spectral techniques such as FT-IR, Raman, UV–Vis and analytical tools such as XRD, FE-SEM, TEM, EDX, XPS and BET. In view of Ag2O NPs as electrode material with wide technological applications, the recovered Ag2O NPs were examined for their sensing and supercapacitive behavior. The developed sensor was explored to detect 2, 4, 6-trichlorophenol, and as expected it shows moral parameters which are required of an effective sensor. Therefore, it was exploited for the quantification of 2, 4, 6-trichlorophenol in soil samples from the agricultural area. Cyclic voltammetric (CV), Galvanostatic Charge-Discharge (GCD) and Electrochemical Impedance Spectroscopic (EIS) studies on the recovered Ag2O NPs coated Ni foam electrode depicted the pronounced capacitive behavior. The GCD studies revealed an enhanced electrochemical performance, particularly with the large specific capacitance of 530 F/g at a current density of 1 A/g. The cyclic stability of the electrode material was identified with 88% retention in specific capacitance even after 5000 GCD cycles. These results strongly proved that the recovered Ag2O NPs are potential candidates for sensing and supercapacitor applications.

Synthesis, characterization, docking and electrochemical studies of nitroaromatic amides

A series of N-[4-(Nitrophenoxy)phenyl]alkanamides (N-1 to N-14) with different alky groups were synthesized following two-step procedures. The structural elucidation of the synthesized compounds was carried out by IR and NMR spectroscopic techniques along with elemental and single crystal X-ray diffraction analyses. Molecular docking study (drug-DNA binding) was carried out to check their biological potential. The docking data revealed the interaction of compounds with ds. DNA, which was further complemented by cyclic voltammetric study. The synthesized compounds showed minor groove binding mode of interaction with DNA. The increased chain length of alkyl groups did not significantly affected the compound-DNA binding.

Preparation of Co-MOF derived Co(OH)2/multiwalled carbon nanotubes as an efficient bifunctional electro catalyst for hydrazine and hydrogen peroxide detections

Abstract Metal–organic frameworks (MOFs) are the more desirable electrode materials in the recent development of electrocatalyts. Still, the electrocatalytic behavior as well as the stability of the prepared MOFs were diminished by its poor conductance. In this work, Cobalt hydroxide (Co (OH)2) functionalized multi-walled carbon nanotubes (MWCNTs) with enhanced electro activity has been prepared using Co-based metal−organic framework (Co MOF, ZIF-67) templates through room temperature electrochemical synthesis. The as synthesized composite has been utilized in the construction of MWCNTs-Co(OH)2 modified glassy carbon electrode (GCE) for the determination of hydrazine (N2H4) and hydrogen peroxide (H2O2). The higher electrochemical performance of MWCNTs-Co(OH)2/GCE compared to individual MWCNTs/GCE and Co(OH)2/GCE was evident from the cyclic voltammetric studies. The characterizations of the composite have been carried out using various techniques. The electrochemical parameters such as linear range, limits of detection and sensitivity of MWCNTs-Co(OH)2/GCE were found to be more comparable with the previous N2H4 and H2O2 amperometric sensors reported in the literatures. The outcomes of practicability inspections were adequate with the recovery results of conventional methods. The high selectivity of MWCNTs-Co(OH)2 modified GCE towards N2H4 and H2O2 has been further demonstrated in the presence of several biologically interfering molecules.

Synthesis, Photophysical, Electrochemical Properties, DFT Studies and DSSC Performance of BODIPY Cored Triazole Bridged 3,6‐Ditertiary Butyl Carbazole Decorated Dendrimers

AbstractA series of BODIPY cored dendrimers up to second generation decorated with 3,6‐ditertiary butyl carbazole at the surface and triazole as the bridging unit has been achieved by convergent approach. The synthesised dendrimers show excellent photophysical and electrochemical properties. All the dendrimers exhibit bi‐exponential lifetime decay, with the higher generation dendrimers (G2) exhibiting longer lifetime decay than the lower generation dendrimers. Density functional theory calculations on the synthesized dendrimers show the HOMO – LUMO energy gap as 2.42 eV with predicted absorption maximum around 425 nm. Cyclic voltammetric studies reveal that all the dendrimers exhibit irreversible oxidation‐reduction signal. Higher generation dendrimer (G2) shows a better power conversion efficiency of 2.7% than the other synthesised dendrimers when used as sensitizer in DSSC.

Pronounced luminescence efficiency and thermal stability of small imidazole architect 2-(1, 4, 5-triphenyl-1H-imidazol-2-yl)phenol for efficient non-doped blue OLEDs

A remarkable attention have been devoted to fabricate blue OLEDs, since it plays a vital role in the field of organic electronics particularly in full colour flat panel displays. Though, notable research outputs were emerged, it is still being a challenging task by the researchers to fulfil its essential criteria such as highly efficient, colour stable and long life-span materials. With the view to address the issues, herein we report an easy synthetic strategy under catalyst free condition for a single imidazole moiety, 2-(1, 4, 5-triphenyl-1H-imidazol-2-yl)phenol, (TSPI-1) with excellent emissive behavior and high thermal stability. OLED device utilizing TSPI-1 as an active layer with the configuration of ITO/PEDOT: PSS (40 nm) /TSPI-1 (60 nm)/ TPBi (30 nm) / LiF (1 nm)/Al (100 nm) displayed blue emission with the maximum brightness of 1.60 Cd/m2, CIE coordinates (0.17, 0.18) and the turn on voltage of 3.77 V. The synthesized imidazole derivative, TSPI-1 was well characterized by FT-IR, UV–vis, photoluminescence (PL), 1H &13C NMR, TGA-DTA, mass spectrometry and cyclic voltammetric (CV) studies. The results revealed the existence of localized electron density through non-planar geometry, specifically in solid state, good decay time, better morphological stability, (Tg = 111.93 °C) and high decomposition temperature (Td = 344 °C), therefore rendering it as a highly desirable material to fabricate OLED device under vacuum evaporation.

Electrochemical Diagnosis of Chemical Switch: Impact of Structural Changes on Charge Transport Mechanism of “Redox Anion Bound Polysilsesquioxane” Film

AbstractHerein, we report the structurally dependent charge transport mechanism of ferrocyanide ions immobilized in a protonated amine functionalized polysilsesquioxane (PSQ) film (Redox polymer film). This redox polymer film shows anion actuated reversible “active to inactive” electrochemical interfaces when the electrolyte anion is changed from chloride to perchlorate as a result of swelling and hydrophobic collapse of the PSQ film, respectively. This concept was successfully applied for switchable electrocatalytic oxidation of ascorbic acid by a [Fe(CN)6]4−‐PSQ film. In addition, it was shown in cyclic voltammetric (CV) studies that the presence of HPO42− and CO32− is leading to strong irreversible structural collapse of the PSQ film, whereas, the presence of SO42− and S2O82− is leading to reversible structural changes of the PSQ film within chloride ion medium. Reversible structural transition between swelling and aggregation (voltammetric response reversibly changing from peak to sigmoidal shape) described in this work is new and provides mechanistic insight into redox polymer electrochemistry and allows differentiating electron hopping from physical motion of redox polymers. Moreover, ion‐exchange between bound redox anions and divalent anions in the electrolyte at fixed positive surface sites of PSQ film was explained in terms of Jones‐Dole B‐coefficient. Electrochemical impedance spectroscopy, Atomic force microscopy, Contact angle measurement and UV‐visible spectroscopy analysis of above phenomenon were consistent with CV results.

Synthesis, Characterization, DNA Binding and Nuclease Activity of Cobalt(II) Complexes of Isonicotinoyl Hydrazones

Cobalt(II) complexes of isonicotinoyl hydrazones of two series of ligands have been synthesized and characterized on the basis of elemental analyses, molar conductance, magnetic moment, mass, IR, UV spectral data. Electrochemical behavior of ligands and complexes has been investigated by using cyclic voltammetry. Cyclic voltammetric studies reveal that the oxidation/reduction potentials of all complexes are shifted towards positive or negative values than its corresponding ligands. The interactions of these complexes with calf thymus DNA have been studied by using absorption studies. Bathochromic shift and hypochromism suggest that the intercalative mode of binding of complexes with DNA. Their DNA cleavage activity of complexes was studied on double-stranded pBR322 plasmid DNA using gel electrophoresis experiments in the absence and presence of oxidant (H2O2) and reductant (DTT).

Impact of graphene on the enhancement of electrochemical and photocatalytic performance of Gd2O3 - Graphene nanocomposites

Gadolinium oxide - Graphene (Gd2O3-G) nanocomposites were prepared with different weight ratio of graphene using low temperature solution process. The structural, morphological, electrochemical and photocatalytic properties of the composites were investigated by X-ray diffraction, Raman, FE-SEM, HRTEM, Cyclic voltammetric and photo-degradation analysis. The chemical composition of the composites was studied by elemental mapping analysis using EPMA. The binding states of various elements present in the composites were analyzed by XPS. Cyclic voltammetric studies revealed that the nanocomposite with 5% graphene exhibits the specific capacitance of 26 F g−1, which is higher than that of pure Gd2O3 (18 F g−1). The presence of graphene has greatly enhanced the photocatalytic performance of Gd2O3G composites as the rate of degradation of MB dye is relatively higher in the composites compared to pure Gd2O3. The significant increase in the specific capacitance and rate of degradation of dye suggest that the Gd2O3G is a promising material for energy storage and environmental applications.

Preparation and preliminary evaluation of a tris-metronidazole-99mTc(CO)3 complex for targeting tumor hypoxia

Conventional 99mTc-radiopharmaceuticals for the detection of tumor hypoxia generally possess a single nitroimidazole moiety. Herein, we report the synthesis and evaluation of a 99mTc-complex with three-nitroimidazole moieties in an attempt to improve hypoxic cell detection. Isocyanide derivative of metronidazole (MetroNC) was synthesized and subsequently radiolabeled with [99mTc(CO)3(H2O)3]+ precursor complex, wherein the three labile water molecules were replaced with MetroNC ligand to form a pseudo-octahedral [99mTc(CO)3(MetroNC)3]+ complex. Analysis of corresponding Re(CO)3-analog prepared in macroscopic scale confirmed the formation of expected complex. Cyclic voltammetric studies of [Re(CO)3(MetroNC)3]+ complex showed no significant change in single-electron reduction potential (SERP) of MetroNC ligand (− 0.96 V) upon forming the [Re(CO)3(MetroNC)3]+ complex (− 0.90 V). In vitro studies in Chinese hamster ovary (CHO) cells showed three-fold preferential accumulation of [99mTc(CO)3(MetroNC)3]+ complex in hypoxic cells over normoxic cells. Biodistribution studies of [99mTc(CO)3(MetroNC)3]+ complex in Swiss mice bearing fibrosarcoma tumor showed tumor uptake and steady retention till 60 min post injection. Present study constitutes a novel design approach towards development of a 99mTc-radiopharmaceutical for hypoxia imaging application, which could be extended to other potential nitroimidazole ligands.

Spectroscopic investigations on La3+, Pr3+, Nd3+ and Gd3+ complexes with a multidentate ligating system: Luminescence properties and biological activities

A series of eight complexes of Ln chlorides and perchlorates (Ln = La3+, Pr3+, Nd3+ and Gd3+) with the ligand, L2− (H2L = 2,2′-iminobis [N-(3-hydroxypropyl)acetamide]), are prepared and characterized by spectral (FTIR, FAB mass, and UV–Visible, 1D and 2D NMR), thermal, electrochemical and density functional theory (DFT) studies. The analytical and spectral data confirmed the composition to be [LnLCl(H2O)2]·2H2O {La3+(1), Pr3+(2), Nd3+(3) and Gd3+(4)} and [LnLClO4(H2O)2]·H2O {La3+(5), Pr3+(6), Nd3+(7) and Gd3+(8)}. The spectral data ascertained a hepta-coordinate geometry of the complexes, which was further supported from DFT studies. Analytical and thermal data supported the stoichiometry of the complexes. The oscillator strengths and the covalency parameters (β, b1/2 and δ) were evaluated from electronic (UV–Vis) spectral studies. The complexes exhibited moderate emission in the luminescence spectra of Pr3+ and Nd3+. Cyclic voltammetric studies confirm the formation of quasi-reversible (PrIII/IV and GdIII/IV) redox couples in solution. Complexes and the free ligand were tested for antibacterial and antifungal properties. Molecular docking results ascertain the comparable binding affinity of the neutral ligand (H2L), complex (4) and complex (8) with B-DNA.

Facile Separation, Spectroscopic Identification, and Electrochemical Properties of Higher Trifluoromethylated Derivatives of [70]Fullerene.

We survey the structure and electronic properties of the family of higher trifluoromethylated C70 (CF3 )n molecules with n=14, 16, 18, and 20. Twenty-two available compounds, of which thirteen are newly obtained and characterized, demonstrate the broad diversity of π-system topologies, which enabled us to study the interplay between the CF3 addition pattern and the electronic properties. UV/Vis spectroscopic and cyclic voltammetric studies demonstrate the importance of the exact addition pattern rather than the plain number of addends. Of particular interest is the skew pentagonal pyramid (SPP) addition pattern, which enables formation of closed-shell cyclopentadienyl anions C70 (CF3 )n-1- through CF3 detachment upon electron transfer. A detailed study of the process is presented for a SPP-C70 (CF3 )16 where potentiostatic electrolysis at the second reduction potential gives C70 (CF3 )15- oxidizable to a persistent C70 (CF3 )15. radical. Together with the literature data for the lower C70 (CF3 )n compounds with n=2-12, the present results show good correlation between the experimental boundary level positions and the DFT predictions. The compounds turn out to be electron acceptor molecular semiconductors with experimental LUMO energies and HOMO-LUMO gaps within the ranges of -4.3 to -3.7 eV and 1.6 to 3.3 eV, respectively, depending on the shape of the conjugated fragments. The HOMO levels fall within the range of -5.6 to -6.9 eV and show linear correlation with the number of addends.

A Combined XRD, Solvatochromic, and Cyclic Voltammetric Study of Poly (3,4-Ethylenedioxythiophene) Doped with Sulfonated Polyarylethersulfones: Towards New Conducting Polymers.

Despite the poor solubility in organic solvents, poly (3,4-ethylenedioxythiophene) (PEDOT) is one of the most successful conducting polymers. To improve PEDOT conductivity, the dopants commonly used are molecules/polymers carrying sulfonic functionalities. In addition to these species, sulfonated polyarylethersulfone (SPAES), obtained via homogeneous synthesis with different degrees of sulfonation (DS), can be used thanks to both the tight control over the DS and the charge separation present in SPAES structure. Here, PEDOTs having enhanced solubility in the chosen reaction solvents (N,N-dimethylformamide, dimethylacetamide, dimethyl sulfoxide, and N-methyl-2-pyrrolidone) were synthesized via a high-concentration solvent-based emulsion polymerization with very low amounts of SPAES as dopant (1% w/w with respect to EDOT monomer), characterized by different DS. The influence of solvents and of the adopted doping agent was studied on PEDOT_SPAESs analyzing (i) the chemical structure, comparing via X-ray diffraction (XRD) the crystalline structures of undoped and commercial PEDOTs with PEDOT_SPAES’ amorphous structure; (ii) solvatochromic behavior, observing UV absorption wavelength variation as solvents and SPAES’ DS change; and (iii) electrochemical properties: voltammetric peak heights of PEDOT_SPAES cast onto glassy carbon electrodes differ for each solvent and in general are better than the ones obtained for neat SPAES, PEDOTs, and glassy carbon.

Determination of Ophthalmic Drug Proparacaine Using Multi-walled Carbon Nanotube Paste Electrode by Square Wave Stripping Voltammetry.

Proparacaine, one of the most common local anesthetics to facilitate diagnosis and treatment of eye diseases, was assayed by square wave voltammetry using a paste electrode prepared with carbon nanotubes. In cyclic voltammetric studies, proparacaine has exhibited a single irreversible anodic peak at around + 900 mV vs. Ag/AgCl in pH 6.0 Britton-Robinson buffer solution. It was suggested that the peak had appeared due to the oxidation of the NH2 group on the proparacaine molecule. Prior to the determination of the proparacaine by square wave stripping voltammetry (SWSV) on the fabricated multi-walled carbon nanotube paste electrode (MWCNTPE), the accumulation potential (Eacc), accumulation time (tacc), pulse amplitude (ΔE), step potential (ΔEs) and frequency (f ) parameters were optimized. The peak currents plotted in the range of 0.5 - 12.5 mg/L proparacaine exhibited two linear sections with a detection limit of 0.11 mg/L. The results for the determination of proparacaine on a pharmaceutical local anesthetic (Alcaine®) showed that relative standard deviation (RSD) and relative error (RE) were 4.1 and -2.0%, respectively. Selectivity has also been investigated and results showed recoveries of 5.0 mg/L proparacaine in the presence of 5.0 mg/L dopamine, ascorbic acid and uric acid as 106.9 ± 0.8, 99.9 ± 1.2 and 94.1 ± 0.7, respectively.

Synthesis and electrolytic cation-dependent cyclic voltammetric study of SILAR deposited PPy-Cr2O3 in equimolar aqueous solutions of H2SO4, Na2SO4, and K2SO4

PPy-Cr2O3 hybrid flexible electrodes (HFEs) have been synthesized by successive ionic layer adsorption and reaction (SILAR) method using flexible aluminum (Al) strips (derived from cold drink cans) as substrates. For the synthesis, 0.1 M pyrrole and 0.02 M K2Cr2O7 dissolved in 0.5 M aqueous H2SO4 were used as precursors while the flexible aluminum strips derived from waste cold drink cans were used as substrates. XRD pattern shows the peak at 50.48^circ indicating the existence of rhombohedral Cr2O3 in the hybrid. FTIR spectrum corroborates the formation of hybrid. SEM image exhibits porous morphology with interconnected granules. TEM image depicts the Cr2O3 granules of average size 20 nm and PPy globules of average size 50 nm. The liquid–solid contact angle was found to be 10^circ 30{prime } indicating the near-superhydrophilic nature of HFE. Cyclic voltammetric (CV) analyses of HFEs have been carried out in each of 20 ml, 0.5 M solutions H2SO4, Na2SO4, and K2SO4. Changes in potential window, redox behavior and hence, the specific capacitance have been observed. The pseudocapacitance of HFE is combined effect of doping–dedoping of PPy matrix with SO42− anions as well as redox reaction shown by Cr2O3. HFE shows maximum specific capacitance 4246 Fg−1 in K2SO4 as measured from CV. HFE shows appreciable stability with 58.20% retention in capacitance even after 1000 cycles.

Selectivity for La3+ ion by synthesized 4-((5-methylfuran-2-yl)methylene)hydrazono)methyl)phenol receptor and its spectral analysis

The functionalized molecules with specific molecular sites appear to be a promising approach for detection of cation in UV–visible and fluorescence spectroscopy. The synthesized receptor 4-((5-methylfuran-2-yl)methylene)hydrazono)methyl)phenol MFMHMP was found selective for La3+ among Ag+, K+, Na+, Be2+, Mg2+, Ca2+, Eu3+, Al3+, La3+, Zr4+, Th4+, UO22+, Fe3+, Fe2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+ metal ions used as their nitrates by UV–visible spectroscopy and fluorescence spectroscopy. The binding nature of MFMHMP with La3+ ion was analyzed by UV–visible, fluorescence, IR, mass spectroscopy and cyclic voltammetric studies. The stoichiometry was established to be 1:1 by Benesi-Hildebrand, mole-ratio method and method of continuous variation (Job's method) with good association affinity K = 6.245 × 104 M−1. Computational studies and Density functional theory (DFT) calculation gives the proof of electron transfer during excitation and emission. Binding energy of complex through Density Function Theory −62.387 kcal/mol has also indication of strong binding. The electron transfer energy of Higher occupied molecular orbital (HOMO) to Lower unoccupied molecular orbital (LUMO) is about 4.662 eV for MFMHMP+La3+ Complex. Among that all transitions HOMO → LUMO + 8 and HOMO → LUMO + 9 play a key role for the blue shift transition during complexation.

Binary Thiolate DNA/Ferrocenyl Self-Assembled Monolayers on Gold: A Versatile Platform for Probing Biosensing Interfaces.

The properties of DNA self-assembled monolayers (SAMs) have strong influences on the interfacial DNA-analyte binding behavior, which further affect the performance of biosensors built upon. In this work, we prepared binary thiolate DNA/6-ferrocenyl-1-hexanethiol (FcC6SH) SAMs on gold (DNA/FcC6S-Au) for convenient electrochemical characterization and subsequent data analysis. Our cyclic voltammetric (CV) studies confirmed that the redox responses of surface-tethered Fc and electrostatically bound [Ru(NH3)6]3+ are capable of providing quantitative information regarding the DNA film properties, including the surface density, structural heterogeneity, and molecular orientation under different preparation and measurement conditions. With the binary thiolate DNA/FcC6S-Au SAM prepared in the conventional post-assembly exchange protocol as a trial system, we are demonstrating the capability of introducing redox-active thiols as passivating and labeling reagents for preparing many other DNA-based biosensing interfaces via varied assembly steps and under different measurement conditions.