Cumulative Intake Research Articles

Assessment of Radiation Background Suppression Using Phoswich Detectors for In Vivo Pb-210 Measurements: A Simulation Study

Detecting the activity of 210Pb in the human skull by counting its 46.5-keV gamma rays in vivo is a promising method to reconstruct one’s cumulative radon intake, based on which associated lung cancer risk can be evaluated. However, this technique is strongly challenged by the background radiation level, which can be largely categorized as room background and subject background. In this work, we quantitatively assess the performance of the phoswich detector in suppressing background radiation resulting from 40K ubiquitously present in human subjects under in vivo measurements using Monte Carlo simulations. We first determined the region of interest for 210Pb gamma-ray detection to be 31 to 61 keV and focused on the background level inside this region caused by two 40K decay processes. It is found that the 1.46-MeV gamma-ray–led background can be reduced by 40% by the phoswich detector operating in anticoincidence mode whereas the 1.31-MeV beta-particle–led background is almost unaffected. This observation is understood through the dependence of the anticoincidence efficiency on the incident gamma-ray energies. Our results suggest that the 1.31-MeV beta-particle–led background is much larger and harder to suppress than the 1.46-MeV gamma-ray–led background, and they call for more investigations in the background reduction techniques for 210Pb in vivo measurement.

Alcohol intake and risk of glioma: results from three prospective cohort studies.

The association between alcohol intake and glioma remains unclear. We evaluated the association between alcohol intake and incidence of glioma in three large, prospective cohort studies with repeated alcohol assessments. We harnessed data from three studies with repeat alcohol assessment to compute hazard ratios (HR) and 95% confidence intervals (CI) for glioma by overall alcohol intake and intake from specific beverages using Cox proportional hazards regression, adjusted for age, cohort, body mass index, smoking status, and caloric intake. Analyses were conducted separately for glioma overall and for glioblastoma (GBM). We confirmed 554 incident glioma cases (362 GBM) among 237,505 participants with 6,216,378 person-years of follow up. Cumulative average alcohol intake was associated with reduced risk of glioma (HR = 0.75, 95%CI:0.56-0.99 comparing > 8-15 to ≤ 0.5g/d; HR = 0.71, 95%CI:0.53-0.96 comparing > 15g/d to ≤ 0.5g/d). When stratified by sex, for the same comparisons, the HRs for men were 0.57 (95%CI:0.36-0.89) and 0.79 (0.53-1.16), and for women 0.90 (95%CI:0.62-1.30) and 0.62, 95%CI:0.39-0.97. Results were consistent when examining cumulative average, baseline, and recent intake, and with a 4year lag. These results provide evidence against a positive association between alcohol intake and glioma risk. Alcohol intake was associated with reduced risk of glioma in both men and women.

Welfare and performance of slower growing broiler breeders during rearing

Current commercial strains of broiler breeders can only achieve an optimal reproductive performance under feed restriction. However, chronic feed restriction in broiler breeders is a welfare concern because of physiological and behavioral signs of hunger, lack of satiety, and frustrated feeding motivation. The objective of this research was to assess the welfare and performance of slower growing broiler breeders during rearing. A total of 360 broiler breeder chicks from 3 female strains (100 chicks per strain) and 2 male strains (20 and 40 chicks per strain) were raised in four identical pens per strain. Strain B and C pullets and X cockerels were slower growing strains, and strain A pullets and Y cockerels were intermediate growing strains. Birds were weighed and scored individually for footpad lesions, hock burns and feather coverage. Data were analyzed using generalized linear mixed models with pen nested in the models and age as a repeated measure. Compared to B and C pullets, strain A pullets grew faster, had poorer body weight uniformity, and started feed restriction 2 wk earlier to control growth rate. Strain A pullets also had higher feeding rate at 3 and 5 wk, higher water intake at 4 and 5 wk, and higher prevalence of footpad lesions at 6 wk than the other pullet strains. Fault bars in wing feathers (an indicator of chronic stress) were more numerous in A pullets than in B and C pullets. Our results indicate that pullets showed little feather coverage loss during early rearing and had good body weight uniformity and low cumulative feed intake at the end of rearing. Slower growing broiler breeders may still require some degree of feed restriction to control growth rate, and strains with lower feed restriction exhibited lower signs of feeding frustration and high body weight uniformity.

Higher Dietary Se Intake Is Associated With the Risk of New-Onset Fracture: A National Longitudinal Study for 20 Years.

Background: The association between dietary selenium (Se) intake and osteoporosis-related fractures remains inconsistent. We aimed to examine the dose relationship between Se intake and incident fracture among Chinese adults.Methods: The dietary data were retrieved from the China Health and Nutrition Survey conducted between 1991 and 2011, and 17,150 participants aged above 20 were included. A 3-day, 24-h recall of food intake was performed to assess cumulative average dietary Se intake. The fracture was based on self-report in each survey between 1997 and 2011. The association between Se intake and fracture was tested by Cox regression, and the non-linear association was examined by restricted cubic splines (RCS).Results: There were 976 fracture cases during a mean of 10.2 years follow-up. In a fully adjusted Cox model, across the quartiles of Se intake, the hazard ratios (HRs) for fracture were 1.07 (95% CI .86–1.33), 1 (reference), 1.25 (95% CI 1.02–1.53), and 1.33 (95% CI 1.07–1.65). RCS showed a parabolic association (P non-linear = 0.037) between Se and fracture for men as well as a U-shape dose-response (P non-linear = 0.04) between Se and fracture for subjects living in highly urbanized areas.Conclusion: In conclusion, there is a non-linear association between selenium intake and fracture, with higher intake associated with increased risk. The shape of the association varies by gender and urbanization level.

Plant-Centered Diet and Risk of Incident Cardiovascular Disease During Young to Middle Adulthood.

BackgroundThe association between diets that focus on plant foods and restrict animal products and cardiovascular disease (CVD) is inconclusive. We investigated whether cumulative intake of a plant‐centered diet and shifting toward such a diet are associated with incident CVD.Methods and ResultsParticipants were 4946 adults in the CARDIA (Coronary Artery Risk Development in Young Adults) prospective study. They were initially 18 to 30 years old and free of CVD (1985–1986, exam year [year 0]) and followed until 2018. Diet was assessed by an interviewer‐administered, validated diet history. Plant‐centered diet quality was assessed using the A Priori Diet Quality Score (APDQS), in which higher scores indicate higher consumption of nutritionally rich plant foods and limited consumption of high‐fat meat products and less healthy plant foods. Proportional hazards models estimated hazard ratios of CVD associated with both time‐varying average APDQS and a 13‐year change in APDQS score (difference between the year 7 and year 20 assessments). During the 32‐year follow‐up, 289 incident CVD cases were identified. Both long‐term consumption and a change toward such a diet were associated with a lower risk of CVD. Multivariable‐adjusted hazard ratio was 0.48 (95% CI, 0.28–0.81) when comparing the highest quintile of the time‐varying average ADPQS with lowest quintiles. The 13‐year change in APDQS was associated with a lower subsequent risk of CVD, with a hazard ratio of 0.39 (95% CI, 0.19–0.81) comparing the extreme quintiles. Similarly, strong inverse associations were found for coronary heart disease and hypertension‐related CVD with either the time‐varying average or change APDQS.ConclusionsConsumption of a plant‐centered, high‐quality diet starting in young adulthood is associated with a lower risk of CVD by middle age.

Elucidating the impact of feeding behaviour on body composition in finishing pigs fed ad libitum using an integrative feeding behaviour index

Large variability in body composition is observed in pigs fed and raised in similar conditions. Part of this variation might be explained by the feeding behaviour of pigs, given its effect on metabolic hormones associated with energy regulation and protein metabolism. The objective of this study was to elucidate this relationship by examining body composition with a new index describing feeding behaviour in finishing pigs. Feeding behaviour and body composition information on 138 pigs during the last 28 d of the finishing phase from three trials were used. For each pig, the sum of the areas between the observed relative cumulative feed intake and the regression line for the evolution of relative feed intake over time was used to calculate the new feeding behaviour index. This index quantifies the irregularity of feed intake (IIFI) by integrating different components of feeding behaviour into a single measurement. Body lipids and protein composition were estimated by dual X-ray densitometry at the beginning (day 1) and end of the finishing phase (day 28). The relationship of IIFI with performance and body composition variables was examined using correlation tests and factor analysis within each dataset. In datasets where IIFI was correlated with body composition, linear models were used to quantify the contribution of IIFI to the total variation of body composition. Weak to moderate correlations (r = ±0.31 to ±0.44; P < 0.05) of IIFI with body composition and performance variables were found only in datasets 1 and 2. However, in dataset 2, IIFI was also correlated with daily feed intake (r = - 0.54; P < 0.001). Irregular feeding behaviour is associated to increased proportion of protein (PdDG) and decreased proportion of lipids (LdDG) in body gain. However, IIFI only accounted for 8% to 14% and 10% to 12% of the total variation of PdDG and LdDG in datasets 2 and 1, respectively. Additionally, factor analysis showed that the number of meals and IIFI had high loadings in a factor separate from factors associated with body composition or performance, suggesting that body composition and performance are independent of feeding behaviour. In conclusion, factors other than feeding behaviour might be involved in modulating body composition of finishing pigs fed ad libitum.

Effect of in ovo feeding of probiotic, prebiotic and synbiotic to Broiler embryos on growth performance, Mucin-2 gene expression and gut colonization of microbiota

Supplementation of antibiotics in poultry diet was banned in several countries due to development of antibiotic resistance. In ovo feeding of prebiotics, probiotics and synbiotics have gained more attention recently. The present study was carried out in 2018 to investigate the effect of in-ovo feeding of probiotic, prebiotic and symbiotic on growth performance and gut microbiome of broiler chicken. On 18th day of incubation, 600 eggs were randomly divided into five treatments each with four replicates of 30 eggs each and were injected with different bio-active compounds, viz. 0.2 ml of Lactobacillus acidophilus 3×107 cfu, 0.5% Mannan-oligosaccharide (MOS), synbiotic (0.1 ml each of Lactobacillus acidophilus 3×107 cfu and 0.5% MOS) along with injected and non-injected controls. After hatch, 400 chicks were sorted out as per treatment with four replicates of 20 chicks each. Birds were reared under deep litter system and fed with experimental diet ad lib. In ovo feeding of Lactobacillus acidophilus, MOS either separately or in combination significantly improved hatch weight, fifth week body weight and gain. However, hatchability, cumulative feed intake, cumulative FCR and cumulative livability were not affected. Improved colonization of Lactobacillus acidophilus and suppressed colonization of Salmonella, Escherichia coli and Staphylococcus in all intra-amniotic groups was noticed. Ileal Mucin-2 gene was significantly up-regulated in the order of MOS, L. acidophilus and synbiotic injected broilers. The results concluded that the in ovo delivery of Lactobacillus acidophilus and MOS either separately or in combination had beneficial effect on growth and gut health of broiler chicken.

A novel feeding behavior index integrating several components of the feeding behavior of finishing pigs

Describing the feeding behavior of pigs is difficult given the large day-to-day variations observed for a given animal. The objectives of this study were to create an index that integrates the information from several components of feeding behavior in order to account for intra-animal variation within a day and between days, and to evaluate the capability of this index to study the impact of relevant nutritional factors affecting feeding behavior. Feed intake information from 160 pigs during the last 28 d of the growing phase from three studies was used. For each pig, the sum of the absolute values of the deviation areas between the regression line of the relative cumulative feed intake and the observed cumulative feed intake was used to calculate the weekly index measuring the irregularity of feed intake (IIFI). Spearman’s correlations of IIFI with the number of daily meals (r = −0.42; P < 0.001), meal duration (r = 0.38; P < 0.01), and feed intake per meal (r = 0.41; P < 0.01) indicate that pigs with high IIFI have fewer meals of longer duration and higher feed intake compared to pigs with low IIFI. This shows that IIFI captures information from several components of feeding behavior. Analysis of variance showed no effect of treatment on feeding behavior for datasets 1 and 2. However, the correlation between IIFI and dietary levels of CP (r = 0.34) indicates that diets high in CP were associated with pigs having more irregular meals. In dataset 3, pig feeding behavior was more regular in control diets than in pigs fed fibrous diets (IIFI; 164 vs. 197, respectively; P < 0.05). Additionally, IIFI was smaller in pigs fed canola by-product diets than in pigs fed wheat by-product diets, indicating that the source of fiber may also influence the feeding behavior of pigs. In most cases, IIFI was more effective at identifying differences in feeding behavior between dietary treatments than conventional feeding behavior variables. These results show the ability of IIFI to integrate information from several conventional components of the feeding behavior of pigs and its potential to successfully evaluate the effect of nutritional factors on feeding behavior.

Effect of combination of peripheral oxytocin and naltrexone at subthreshold doses on food intake, body weight and feeding-related brain gene expression in male rats

In a recent case report involving a male with hypothalamic obesity, concurrent administration of oxytocin (OT) and an opioid receptor antagonist, naltrexone (NTX), synergistically affected energy balance. Here, by using laboratory rats, we examined whether the reported synergy between OT and NTX in the context of food intake extends beyond that one unique case. We found that intravenous OT+NTX combination, at doses subthreshold for each of the drugs individually, decreased episodic consumption of a 10% sucrose solution in non-deprived animals. Daily administration of OT and NTX just before a scheduled, 2-hour, high-fat high-sugar (HFHS) meal over 24 days, decreased cumulative HFHS diet intake, but without a change in body weight due to compensatory standard chow intake during the remainder of the day. The NTX-OT treatment affected expression of several feeding-related genes in the hypothalamus, brain stem and nucleus accumbens, brain regions essential for the regulation of energy- and reward-driven consumption. We conclude that OT and NTX act synergistically to decrease food consumption in rats and that this transient effect is accompanied by changes in brain processes relevant to feeding.

Effect of probiotic and nucleotide supplementation in milk replacer on growth performance and fecal bacteria in calves

The aim of the study was to determine whether a combination of both probiotic feed additive and nucleotide supplementation in milk replacer (MR) would have a synergistic impact on growth performance and fecal bacteria of calves. Sixty four 10-day-old dairy calves (40 females and 24 males; 39.3 ± 4.4 kg) were randomly allocated to one of four treatments (10 females and 6 males/treatment) and fed MR (22% CP and 19% fat in DM): 1) without probiotic and additional nucleotides; 2) with probiotic containing spores of Bacillus licheniformis and Bacillus subtilis (0.65 × 106 CFU of each/g of MR); 3) with additional nucleotides (yeast derived; 500 g/ton of MR); and 4) with both probiotic and additional nucleotides. The calves were fed 6 L of MR (150 g of MR powder in 1 L) divided into three feedings and provided texturized starter. Performance and health of calves was monitored for 50 days, including MR intake (daily), cumulative starter intake, body weight (every 10 days), fecal score (daily) and illness (daily). On day 20 and 50 of the study, fecal samples were collected from 8 randomly selected calves/treatment and analyzed for counts of Lactobacillus spp., Bifidobacterium spp., Escherichia coli and Clostridium perfringens. Neither probiotic nor nucleotide supplementation had an effect on feed intake, body weight, average daily gain, and fecal score of calves (P ≥ 0.17). Nucleotide supplementation in MR reduced counts of Lactobacillus spp. in feces on day 20 of the study (P = 0.01) although number of calves with diarrhea also did not differ between treatments (P ≥ 0.53). With doses of feed additives used in the current study, supplementation of both probiotic containing Bacillus spores and nucleotides had no synergistic impact on growth, health, and fecal bacteria of calves; whereas nucleotide supplementation in MR reduced fecal counts of Lactobacillus spp.

Calcium Intake Is Inversely Related to Risk of Obesity among American Young Adults over a 30-Year Follow-Up.

Calcium (Ca) is an essential nutrient that may play an important role in weight maintenance through its involvement in energy or lipid metabolism. However, little is known about the long-term associations of Ca intake with obesity risk. We aimed to prospectively examine the association between cumulative Ca intake and the incidence of obesity among American young adults over 30 y of follow-up. Participants were from the CARDIA (Coronary Artery Risk Development in Young Adults) study. A total of 4097 of 5115 black and white individuals aged 18-30 y at baseline in 1985-1986 were included in the current analysis. Dietary and supplemental Ca intake was assessed by the validated interview-based CARDIA diet history at baseline and exam years 7 and 20. Incident cases of obesity were identified when BMI was ≥30kg/m2 for the first time since baseline. A survival analysis was performed using Cox proportional hazards regression models to estimate the HRs and corresponding 95% CIs for obesity incidence during follow-up. During a 30-y follow-up (mean±SD: 20± 10 y), 1675 participants developed obesity. Cumulative total Ca intake (dietary plus supplemental Ca) was inversely associated with incidence of obesity in multivariable-adjusted analysis [quintile (Q)5 (highest intake) compared with Q1 (lowest intake): HR: 0.68; 95% CI: 0.56, 0.82; P-trend <0.01]. This inverse association persisted among Ca supplement users (Q5 compared with Q1: HR: 0.53; 95% CI: 0.40, 0.70; P-trend <0.01), but was not seen among nonusers. Following a cohort of Americans from young adulthood to midlife, an inverse association between calcium intake and obesity incidence was observed. Further studies are needed to confirm our findings.

Timing and Clinical Significance of Fluid Overload in Pediatric Acute Respiratory Distress Syndrome.

Fluid overload is associated with worse outcomes in adult and pediatric acute respiratory distress syndrome. However, the time-course of fluid overload and its relationship to outcome has not been described. We aimed to determine the relationship between the timing of fluid overload and outcomes over the first 7 days after acute respiratory distress syndrome onset in children. Retrospective cohort study. Single tertiary care PICU. Intubated children with acute respiratory distress syndrome between 2011 and 2019. None. Daily and cumulative total fluid intake, total output, urine output, and fluid balance were collected for each 24-hour period from days 1 to 7 after acute respiratory distress syndrome onset. We tested the association between daily cumulative fluid metrics with PICU mortality and probability of extubation by 28 days using multivariable logistic and competing risk regression, respectively. In a subset of children, plasma was collected on day 1 and day 3 of acute respiratory distress syndrome and angiopoietin-2 quantified. Of 723 children with acute respiratory distress syndrome, 132 died (18%). In unadjusted analysis, nonsurvivors had higher cumulative fluid balance starting on day 3. In multivariable analysis, a positive cumulative fluid balance on days 5 through 7 was associated with increased mortality. Higher cumulative fluid balance on days 4 to 7 was associated with lower probability of extubation. Elevated angiopoietin-2 on day 1 predicted early (within 3 d) fluid overload greater than or equal to 10%, and elevated angiopoietin-2 on day 3 predicted late (between days 4 and 7) fluid overload. Fluid overload after day 4 of acute respiratory distress syndrome, but not before, was associated with worse outcomes. Higher angiopoietin-2 predicted subsequent fluid overload. Our results suggest that future interventions aimed at managing fluid overload may have differential efficacy depending on when in the time-course of acute respiratory distress syndrome they are initiated.

Assessment and Impact of Intravenous Medication Fluid Administration in Critically Ill Patients With Acute Respiratory Failure.

Positive fluid balance early in critical illness is associated with poor outcomes. Reducing intravenous medication volume may mitigate volume overload. Objective: Assessment of fluid and medication administration and clinical outcomes in acute respiratory failure. Single-center, prospective observational study of hemodynamically stable adult patients in a medical intensive care unit (MICU) with acute respiratory failure. Median cumulative total intake volume was 12 890 (interquartile range [IQR] = 8654-22 221) mL (n = 27), and median cumulative intravenous medication volume was 3563 (IQR = 2371-9412) mL over the first 7 days. Medication volume accounted for 27.6% of aggregate fluid volume. Median daily intravenous medication volume administered was 591 (IQR = 339-1082) mL. Cumulative fluid volume was associated with reduced ventilator-free days (r2 = -0.393; P = 0.043), and cumulative fluid volumes during the first 3 and 7 days were associated with increased MICU length of stay (LOS ± standard error 0.73 ± 0.35 d/L, P = 0.047, and 0.38 ± 0.16 d/L, P = 0.021, respectively). Cumulative medication volume administered significantly reduced the likelihood of mechanical ventilator liberation (hazard ratio [HR] = 0.917; 95% CI: 0.854, 0.984; P = 0.016) and MICU discharge (HR = 0.911; 95% CI: 0.843, 0.985; P = 0.019). Small-volume infusion may decrease cumulative intravenous medication volume by 38%. Intravenous medication diluent contributes substantially to total fluid intake in patients with acute respiratory failure and is associated with poor outcomes. Reduction of intravenous medication fluid volume to improve clinical outcomes should be further investigated.

Growth Performance of Grower Pigs (Sus scrofa domesticus L.), Nutritional and Microbial Contents of Wet and Fermented Commercial Hog Ration with Different Levels of Wood Vinegar

Background: Swine is a very important commodity that contributed to the country’s food security by providing about 60% of the total animal meat consumption of the Filipinos. Feeds comprise a major expense in swine production and searching for possibly better and cheaper alternatives is urgent to have high quality but economical animal feeding.Methods: The study aimed to evaluate the growth performance of grower pigs (Sus scrofa domesticus L.), nutritional and microbial contents of wet and fermented commercial ration with different levels of wood vinegar as follows: T0: 0% wood vinegar (WV) per liter Plain water (PW) (3000 mL PW) (control), T1: 2% WV per liter PW (60 mL WV: 2940 mL PW), T2: 5% WV per liter PW (150 mL WV: 2850 mL PW). Data gathered were analyzed using ANOVA and LSD to compare treatment means by STAR version 2.0.1.Result: A significantly higher (p less than 0.05) bi-weekly cumulative feed intake (BWCFI) and bi-weekly cumulative body weight gain (BWCBWG) were noted on grower pigs given wet and fermented commercial hog ration with different levels of WV during week 4 and no significant differences during weeks 2, 6 and 8. No significant differences were noted on ADG and FCR throughout the study. A significantly higher profit was noted on grower-pigs given wet and fermented commercial hog ration 5% WV inclusion. Moreover, no differences in microbial and nutritional contents were noted on fermented feeds. Regardless of treatments, the wet and fermented commercial hog ration revealed the same microbial content present to enumerate as follows: Yeast, Lactobacillus and Streptococcus.

Regional Difference in the Association between the Trajectory of Selenium Intake and Hypertension: A 20-Year Cohort Study.

The effect of selenium on hypertension is inconclusive. We aimed to study the relationship between selenium intake and incident hypertension. Adults (age ≥20 years) in the China Health and Nutrition Survey were followed up from 1991 to 2011 (N = 13,668). The latent class modeling method was used to identify trajectory groups of selenium intake. A total of 4039 respondents developed hypertension. The incidence of hypertension was 30.1, 30.5, 30.6, and 31.2 per 1000 person-years among participants with cumulative average selenium intake of 21.0 ± 5.1, 33.2 ± 2.8, 43.8 ± 3.6, and 68.3 ± 25.2 µg/day, respectively. Region and selenium intake interaction in relation to hypertension was significant. In the multivariable model, cumulative intake of selenium was only inversely associated with the incident hypertension in northern participants (low selenium zone), and not in southern participants. Compared to selenium intake trajectory Group 1 (stable low intake), all three trajectory groups had a low hazard ratio for hypertension among the northern participants. However, Group 4 (high intake and decreased) showed an increasing trend of hypertension risk in the south. In conclusion, the association between selenium intake and the incidence of hypertension varied according to regions in China. In the low soil selenium zone, high selenium intake might be beneficial for hypertension prevention.

Arsenic exposure through drinking groundwater and consuming wastewater-irrigated vegetables in Multan, Pakistan.

Arsenic (As) is one of the most toxic metalloids for humans. Above permissible levels of As cause severe health implications. Contaminated drinking water and food items may be the leading sources of As exposure to people all around the world. The current study assessed the levels of As in drinking water, vegetables, irrigation water, agricultural soils, and the human population (adult women and men) of rural and peri-urban areas of Multan (Pakistan). For a comparison between peri-urban (exposed site) and rural areas (control site), we sampled irrigation water, vegetables and vegetable-grown soils, drinking water, and human blood. In all sample types, As concentration was significantly higher at exposed site than at control site. Alarmingly, As concentration in drinking groundwater (34µg As L-1) of exposed site was 3.4-folds higher than the permissible limit (set by WHO). Among the studied vegetables, the cumulative daily dietary intake of As was recorded maximum by the consumption of okra (474ng d-1 on exposed site) and minimum by long melon (1ng d-1 on control site). However, As intake via drinking water was estimated to contribute ≥ 98% of total As intake at both sites. Hence, the health risks associated with drinking As-contaminated groundwater were recorded much higher than the health risks associated with the consumption of As-contaminated vegetables. Blood As levels in most of the subjects at exposed site exceeded the safe limit of 12µg L-1. Conclusively, the findings of the current study indicated that drinking contaminated groundwater may be the major cause of As-associated health risks in the region.

EFFECT OF ORGANIC ACID AS A FEED SUPPLEMENT ON GROWTH PERFORMANCE OF BROILER

An experiment was carried out to evaluate the effect of organic acid as a feed supplement on growth performance of broiler. The experiments were laid out in Completely Randomized Block design (CRD) with four treatments. The treatments were Control, 0.5% Propionic acid,1% Lactic acid,1% Citric acid. Each treatment was replicated thrice. It was revealed that the effect of organic acid on feed conversion ratio were 1.48,1.49,1.54 and 1.68 in these different treatments control, Propionic acid, Lactic acid and Citric acid respectively. The cumulative feed intake was found to be 3073.94gm, 2911.46gm, 3338.24gm and 3214.94gm in these different treatments control, Propionic acid, Lactic acid and Citric acid respectively. Similarly, water intake were found to be 356.79ml, 350.28ml, 400.97ml and 355.51ml in these different treatments control, Propionic acid, Lactic acid and Citric acid respectively. From the overall experiment, we observed that control feed gave less feed conversion ratio (FCR) though mortality rate was higher. The FCR was found to be less in propionic acid as comparison to other organic acids and the mortality rate was found to be lowest as well. Cumulative feed intake were highest in lactic acid treatment and lowest in propionic acid treatment and water intake were highest in lactic acid treatment and lowest in propionic acid treatment. So, we can conclude that the effect of lactic acid on water intake and feed intake were found to be better whereas the effect of propionic acid was found to be better on feed conversion ratio.

Dietary Gluten Intake Is Not Associated With Risk of Inflammatory Bowel Disease in US Adults Without Celiac Disease

Diet is thought to play a role in the development of inflammatory bowel disease (IBD), though it is unknown whether gluten intake confers risk of IBD. The aim of this study was to determine the relationship between gluten intake and risk of incident Crohn's disease (CD) and ulcerative colitis (UC). We performed a prospective cohort study of 208,280 US participants from the Nurses' Health Study (1986-2016), Nurses' Health Study II (1991-2017), and the Health Professionals Follow-up Study (1986-2016) who did not have IBD at baseline or celiac disease, and who completed semiquantitative food frequency questionnaires. We used Cox proportional hazards modeling to estimate the risk of IBD according to quintiles of cumulative average energy-adjusted dietary gluten intake over the follow-up period. We documented 337 CD cases and 447 UC cases over 5,115,265 person-years of follow-up evaluation. Dietary gluten intake was not associated with risk of IBD. Compared with participants in the lowest quintile of gluten intake, the adjusted hazard ratios and 95% CIs for participants in the highest quintile of gluten intake were 1.16 (95% CI, 0.82-1.64; Ptrend= .41) for CD and 1.04 (95% CI, 0.75-1.44; Ptrend= .64) for UC. Adjusting for primary sources of gluten intake did not materially change our estimates. In 3 large adult US prospective cohorts, gluten intake was not associated with risk of CD or UC. Our findings are reassuring at a time when consumption of gluten has been increasingly perceived as a trigger for chronic gastrointestinal diseases.

Administration of Fermented Averrhoa bilimbi L. Fruit Filtrate on Growth, Hematological, Intestinal, and Carcass Indices of Broilers

The study investigated the effects of administration of graded levels of fermented Averrhoa bilimbi L. fruit filtrate in drinking water on the growth performance, hematological variables, intestinal ecology, and carcass characteristics of broilers. The experiment was arranged based on a completely randomized design. Two hundred day-old-Lohmann broiler chicks were randomly assigned into 4 treatment groups, each consisting of 5 replications with 10 chicks in each replication. The treatments were administration of fermented A. bilimbi L. fruit filtrate through drinking water at concentrations of 0% (CONT), 0.5% (FAB05), 1.0% (FAB1), and 2.0% (FAB2). Blood was sampled on days 21 and 33, while intestinal segments and digesta were collected on day 33. Feed conversion ratio (FCR) was improved (p<0.05) with the increased concentrations of fermented filtrate in drinking water. Body weight, cumulative feed intake, and mortality of broilers were not affected by the treatments. On day 21, thrombocytes decreased (p<0.05) with the increased concentrations of fermented filtrate. On day 33, leukocytes and lymphocytes were lower (p<0.05) in treated chicks than in control. On day 21, the high-density lipoprotein (HDL) and aspartate aminotransferase (AST) increased (p<0.05) with the enhanced fermented filtrate concentrations. On day 33, creatinine and alanine aminotransferase (ALT) increased (p<0.05) following the increased fermented filtrate concentration in drinking water. Fermented fruit filtrate increased (p<0.05) jejunal villi height and ileal crypt depth. Fermented filtrate also resulted in higher (p<0.05) pH values of jejunum. The Enterobacteriaceae counts in the ileum decreased (p<0.05) with the increased fermented filtrate concentration in drinking water. Fermented fruit filtrate decreased (p<0.05) the liver and caeca weights and increased (p<0.05) the proventriculus weight. In conclusion, administration of 2% of fermented A. bilimbi L. fruit filtrate (pH 1.83) through drinking water improved FCR, physiological condition, and intestinal ecology of broilers. The addition of fermented fruit filtrate up to 2% in drinking water did not exert a negative effect on the carcass characteristics of broilers.

Canola meal or soybean meal as protein source and the effect of microencapsulated sodium butyrate supplementation in calf starter mixture. I. Performance, digestibility, and selected blood variables

Two studies were conducted to assess the effect of protein source and microencapsulated sodium butyrate (MSB) inclusion in pelleted starter mixtures on growth performance, gain to feed (G:F) ratio, nutrient digestibility, and selected blood metabolites in calves. In study 1, 28 Holstein bull calves (8.7 ± 0.8 d of age and 43.0 ± 4.4 kg; mean ± SD) were allocated to 1 of 4 treatments in a 2 × 2 factorial arrangement and fed a pelleted starter mixture containing canola meal (CM, 35% as fed) or soybean meal (SM, 24% as fed) as the main source of protein, with or without supplemental MSB (0.3% as fed). Starter mixtures were formulated to be similar for crude protein, Lys, and Met, and were fed ad libitum. Calves were weaned after 42 d of milk replacer feeding (51.7 ± 0.8 d of age) and observed for another 21 d. Furthermore, selected blood metabolites were measured on d 21, 42, and 63 of the study, and nutrient digestibility was measured after weaning. In study 2, 60 Holstein heifer calves (9.1 ± 0.8 d of age and 43.2 ± 4.2 kg) were assigned to the same treatments as in study 1. The calves were weaned after 49 d of milk replacer feeding (59.1 ± 0.8 d of age) and observed for an additional 14 d. Milk replacer and starter mixture intake and fecal score were recorded daily, whereas body weight (BW) was recorded weekly. In study 1, calves fed starter mixtures containing CM had or tended to have lesser preweaning starter intake, weaning average daily gain (ADG), weaning and overall G:F ratio, and postweaning total-tract dry matter digestibility, as opposed to those fed starter mixtures with SM. However, these differences did not affect overall starter intake, overall ADG, or final BW. Supplementation with MSB only tended to increase the preweaning starter mixture intake. In study 2, heifer calves that were fed starter mixtures with CM had greater cumulative starter intake after weaning, but the protein source in the starter mixture had no effect on ADG, BW, or G:F ratio. Inclusion of MSB in starter mixtures for calves tended to decrease postweaning starter mixture intake. In conclusion, use of CM or SM as the main source of protein in starter mixture resulted in similar growth performance of bull and heifer calves; however, CM use in starter mixtures reduced starter intake, ADG, and G:F ratio at least at some points of rearing. Supplementation of MSB had minor effects on the growth performance of calves.