Culture Regeneration Research Articles

Cultural regeneration through urban design in Algeria. The case of Leonardo Fibonacci’s promenade in Bejaia

Cultural regeneration through urban design in Algeria. The case of Leonardo Fibonacci’s promenade in Bejaia

“Places of Memory” as the Historical and Spiritual Values of the Kazakh Culture Regeneration

“Places of Memory” as the Historical and Spiritual Values of the Kazakh Culture Regeneration

Optimization of Protease Activity byBacillus haynesiiBK1H Using Response Surface Methodology

Proteases are enzymes that catalyze the hydrolysis of peptide bonds into oligopeptides and amino acids. Previous bacterial studies reported that eight proteolytic isolates had been isolated from Bledug Kuwu mud, Central Java. One of these isolates is BK1H which has a proteolytic index of 2.960 making it possible to be studied further. In this study, optimization of the protease activity of BK1H was carried out using 3 factors namely temperature, pH, and divalent metal cations. This research was conducted through several stages, (1) Bacterial culture regeneration, (2) Protease fermentation using the SmF method, (3) Determination of the type of protease, (4) One Factor at a Time (OFAT), (5) Plackett-Burman Design (PBD), (6) Response Surface Methodology-Central Composite Design (RSM-CCD), and (7) Confirmation of RSM-CCD optimization results on experimental results. OFAT experiment results showed the lowest activity and the highest activity was obtained at 37°C and 57°C; pH 6 and 8; and CaCl2 0.5 and 1.0 mM. The significance test of the 2 factors namely temperature and pH has a p value <0.05, which indicates that these factors have a significant effect on protease activity. The optimum factor value in the RSM-CCD has a temperature of 63.10°C and pH 10.82, with the predicted activity of 149.910 U/mL, while the experimental results have activity of 157.630 ± 16.240 U/mL.

유휴공간을 활용한 문화재생시설 방문객의 특성이 만족도와 재방문의도에 미치는 영향

This study investigated the influence of cultural regeneration perceptions and socioeconomic attributes on satisfaction and revisit intentions among visitors to Yeongwol Y Park. From the survey, 550 valid responses were secured. The primary outcomes and implications of the research include the following:First, distinct variations in cultural regeneration perceptions were observed across different age groups, occupations, and income levels. To enhance satisfaction and encourage repeat visits, it's imperative to strategize by segmenting visitors based on these attributes. Second, differences in satisfaction and revisit intentions, based on socioeconomic attributes, were evident in certain age and occupational brackets. Third,the role of cultural engagement was deemed significant when analyzing factors contributing to satisfaction. Notably, individuals in their 20s and those within the middle-income range reported higher levels of satisfaction. Fourth, key determinants affecting the intention to return encompassed overall satisfaction, the viewing environment, pricing, and cultural engagement. As a recommendation, the analysis of enhancing the viewing ambiance and refining services are required.

Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine

An investigation was carried out to initiate in vitro mutagenesis in banana cv. Grand Naine shoot tip culture. Based on the sensitivity of explants, the lethal dose was estimated using probit analysis. The LD50 dose of gamma irradiation of the banana shoot tip culture was determined to be 39.5 Gy. It was also discovered that the number of new shoots rose with the number of subculture days, with the largest number of shoots recorded at 40 Gy. The study also discovered that when 13 DAS explants were treated with 10 Gy, they recovered the greatest number of shoots. As a result of the negative effects of higher doses of irradiation, it was decided that determining the lethal dose can be very beneficial in reducing population loss. Its further optimization may be effective in developing significant variations with a certain trait of interest.

Mutual Regulation of Transcriptomes between Murine Pneumocytes and Fibroblasts Mediates Alveolar Regeneration in Air-Liquid Interface Cultures.

Alveolar type 2 and club cells are part of the stem cell niche of the lung and their differentiation is required for pulmonary homeostasis and tissue regeneration. A disturbed crosstalk between fibroblasts and epithelial cells contributes to the loss of lung structure in chronic lung diseases. Therefore, it is important to understand how fibroblasts and lung epithelial cells interact during regeneration. Here, we analyzed the interaction of fibroblasts and the alveolar epithelium modeled in air-liquid interface cultures. Single-cell transcriptomics showed that cocultivation with fibroblasts leads to increased expression of type 2 markers in pneumocytes, activation of regulons associated with the maintenance of alveolar type 2 cells (e.g., Etv5), and transdifferentiation of club cells toward pneumocytes. This was accompanied by an intensified transepithelial barrier. Vice versa, the activation of NF-κB pathways and the CEBPB regulon and the expression of IL-6 and other differentiation factors (e.g., fibroblast growth factors) were increased in fibroblasts cocultured with epithelial cells. Recombinant IL-6 enhanced epithelial barrier formation. Therefore, in our coculture model, regulatory loops were identified by which lung epithelial cells mediate regeneration and differentiation of the alveolar epithelium in a cooperative manner with the mesenchymal compartment.

Epidermal–dermal coupled spheroids are important for tissue pattern regeneration in reconstituted skin explant cultures

Tissue patterning is critical for the development and regeneration of organs. To advance the use of engineered reconstituted skin organs, we study cardinal features important for tissue patterning and hair regeneration. We find they spontaneously form spheroid configurations, with polarized epidermal cells coupled with dermal cells through a newly formed basement membrane. Functionally, the spheroid becomes competent morphogenetic units (CMU) that promote regeneration of tissue patterns. The emergence of new cell types and molecular interactions during CMU formation was analyzed using scRNA-sequencing. Surprisingly, in newborn skin explants, IFNr signaling can induce apical-basal polarity in epidermal cell aggregates. Dermal-Tgfb induces basement membrane formation. Meanwhile, VEGF signaling mediates dermal cell attachment to the epidermal cyst shell, thus forming a CMU. Adult mouse and human fetal scalp cells fail to form a CMU but can be restored by adding IFNr or VEGF to achieve hair regeneration. We find different multi-cellular configurations and molecular pathways are used to achieve morphogenetic competence in developing skin, wound-induced hair neogenesis, and reconstituted explant cultures. Thus, multiple paths can be used to achieve tissue patterning. These insights encourage more studies of “in vitro morphogenesis” which may provide novel strategies to enhance regeneration.

도시유휴공간의 문화적 재생 사례 연구

Vacant urban spaces refer to the abandoned areas resulting from the technological and scientific advancements during the industrial revolution, coupled with urban population concentration. These former secondary industrial facilities lost their original purpose and function as spaces, becoming neglected and left unused. The objective of this study is to identify the current status and vulnerabilities of cultural regeneration in domestic vacant urban spaces and suggests improvement measures. By examining case studies of Le Centquatre and Kulturbrauerei . As a result, the researchers derive various approaches and new directions to enhance the qualitative aspects of cultural regeneration for vacant urban spaces : Firstly, research should focus on developing diverse programs and systems that bridge the gap between practical experience and theoretical knowledge. Secondly, providing spaces that foster aesthetic and fundamental cultural education for children and adolescents, rather than merely instructive art training, is crucial. Thirdly, there is a need to propose and support collaborative programs for artist discovery and development. Fourthly, it is necessary to be aware of the importance of archiving and study various methodologies. Through this study, it is expected that cultural regeneration of vacant urban spaces will be sustainably activated.

TURGAI REGION IN PRE-REVOLUTIONARY WORKS: IN THE CONTEXT OF “NEW IMPERIAL HISTORY”

The article deals with the Russian historiographical tradition of the pre-revolutionary period in relation to the Turgai region. The contribution of the main institutions of this region is analyzed: the Orenburg department of the Imperial Russian Geographical Society, the Turgai regional statistical committee, the Orenburg scientific archival commission. As well as the figures of these institutions, who were also officials of the colonial apparatus: V.V. Katarinsky, I.I. Kraft, A.I. Dobrosmyslov and others, as well as Kazakh researchers. From the position of “new imperial policy” the main reasons of research interest dictated by the study of the region for the purpose of further colonization are shown. Nevertheless, these works are of great informational value. The study allows us to define the activities of pre-revolutionary scientists, both research and service, as cultural regeneration, contrary to the modern assessment of Russian scientists characterizing it as an imperial policy of acculturation.

Investigation of the Influence of Polyamines on Mature Embryo Culture and DNA Methylation of Wheat (Triticum aestivum L.) Using the Machine Learning Algorithm Method.

Numerous factors can impact the efficiency of callus formation and in vitro regeneration in wheat cultures through the introduction of exogenous polyamines (PAs). The present study aimed to investigate in vitro plant regeneration and DNA methylation patterns utilizing the inter-primer binding site (iPBS) retrotransposon and coupled restriction enzyme digestion-iPBS (CRED-iPBS) methods in wheat. This investigation involved the application of distinct types of PAs (Put: putrescine, Spd: spermidine, and Spm: spermine) at varying concentrations (0, 0.5, 1, and 1.5 mM). The subsequent outcomes were subjected to predictive modeling using diverse machine learning (ML) algorithms. Based on the specific polyamine type and concentration utilized, the results indicated that 1 mM Put and Spd were the most favorable PAs for supporting endosperm-associated mature embryos. Employing an epigenetic approach, Put at concentrations of 0.5 and 1.5 mM exhibited the highest levels of genomic template stability (GTS) (73.9%). Elevated Spd levels correlated with DNA hypermethylation while reduced Spm levels were linked to DNA hypomethylation. The in vitro and epigenetic characteristics were predicted using ML techniques such as the support vector machine (SVM), extreme gradient boosting (XGBoost), and random forest (RF) models. These models were employed to establish relationships between input variables (PAs, concentration, GTS rates, Msp I polymorphism, and Hpa II polymorphism) and output parameters (in vitro measurements). This comparative analysis aimed to evaluate the performance of the models and interpret the generated data. The outcomes demonstrated that the XGBoost method exhibited the highest performance scores for callus induction (CI%), regeneration efficiency (RE), and the number of plantlets (NP), with R2 scores explaining 38.3%, 73.8%, and 85.3% of the variances, respectively. Additionally, the RF algorithm explained 41.5% of the total variance and showcased superior efficacy in terms of embryogenic callus induction (ECI%). Furthermore, the SVM model, which provided the most robust statistics for responding embryogenic calluses (RECs%), yielded an R2 value of 84.1%, signifying its ability to account for a substantial portion of the total variance present in the data. In summary, this study exemplifies the application of diverse ML models to the cultivation of mature wheat embryos in the presence of various exogenous PAs and concentrations. Additionally, it explores the impact of polymorphic variations in the CRED-iPBS profile and DNA methylation on epigenetic changes, thereby contributing to a comprehensive understanding of these regulatory mechanisms.

Optimization of High-Efficiency Tissue Culture Regeneration Systems in Gray Poplar.

A series of tissue culture regeneration protocols were conducted on gray poplar (P. tremula × P. alba) to select the most efficient callus induction medium, adventitious shoot differentiation medium, shoot elongation medium and rooting medium, which laid the foundation for the optimization of genetic transformation technology for gray poplar. The results showed that the Woody Plant Medium (WPM) supplemented with 0.10 mg L-1 kinetin (KT) and 1.00 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) was the most suitable medium for callus induction. The callus induction rates of different tissues were greater than 85.7%. The optimal adventitious shoot differentiation medium was the WPM supplemented with 0.02 mg L-1 thidiazuron (TDZ), and the adventitious shoot differentiation rates of young tissues were 22.2-41.9%. The optimal direct differentiation medium was the Murashige and Skoog (MS) medium supplemented with 0.20 mg L-1 6-benzylaminopurine (6-BA), 0.10 mg L-1 indole butyric acid (IBA) and 0.001 mg L-1 TDZ, and the differentiation rate of adventitious shoots was greater than 94%. The best shoot elongation medium for adventitious shoots was the MS medium with 0.10 mg L-1 naphthylacetic acid (NAA). After 45 days of cultivation in the MS medium with 0.10 mg L-1 NAA, the average plant height was 1.8 cm, and the average number of elongated adventitious shoots was 11 per explant. The 1/2 MS medium with 0.10 mg L-1 NAA showed the best performance for rooting, and later, shoot growth. The direct shoot induction pathway can induce adventitious shoots much faster than the indirect adventitious shoot induction pathway can, and the time cost via the direct adventitious shoot induction pathway can be shortened by 2-6 weeks compared to that of the indirect shoot induction pathway.

Development and comparative analysis of initiation ability in large-scale Heuchera propagation using tissue culture versus cuttings

The Heuchera genus, a member of the Saxifragaceae family, encompasses a wide array of varieties and hybrids, serving both traditional medicinal and ornamental purposes. However, a significant knowledge gap persists in achieving efficient mass propagation of diverse Heuchera cultivars creating a substantial market void. To address this, our study focuses on expedited seedling regeneration by investigating leaf cutting and tissue culture techniques to offer novel insights to cultivators. Herein, we successfully rooted thirteen distinct cultivars from the Heuchera and Heucherella (Heuchera × Tiarella) genera through cutting. Moreover, in vitro culture experiments led to the successful induction of calli and shoots from petiole samples. Notably, variations in measured parameters were observed across cultivars in both cutting and tissue culture methodologies. When petiole explants were exposed to cytokinin 6-benzylaminopurine (BA) at concentrations of 0.5, 1.0, and 2.0 mg/L along with auxin α-naphthaleneacetic acid (NAA) at 0.5 mg/L, shoots were produced either directly or indirectly during the primary culture. Exposure to darkness and the application of 2,4-dichlorophenoxyacetic acid (2,4-D) did not promote shoot formation but were beneficial for callus stimulation. Interestingly, a negative correlation was observed between the ease of initiating cutting recovery and inducting tissue culture regeneration, suggesting that cultivars that easily regenerate through cutting might encounter difficulties during induction by tissue culture. In light of these findings, we devised a streamlined and effective protocol for rapid Heuchera propagation. This protocol involves micropropagation, directly acquiring adventitious shoots from primary cultures supplemented by cutting-based propagation methods.

In vitro propagation of Lobelia chinensis Lour. under different LED lights

The current study was performed to verify the effect of light quality especially of light-emitting diodes (LEDs) on the in vitro regeneration of Lobelia chinensis. Nodal explants were cultured on MS medium supplemented with 30 g L-1 sucrose and treated with red (R), blue (B), white (W) combination red/blue (1:1; RB), and red/blue/green (1:1:1; RGB) LED sources. After 4 weeks of culture regeneration capabilities, growth characteristics, and bioactive compounds were evaluated. RB treatments were found suitable for shoot regeneration, shoot growth, and root regeneration from shoots. Photosynthetic pigments, total phenolics, 􀃀avonoid content, and DPPH, ABTS, and FRAP antioxidant activities were highest with plants regenerated under RB LED. HPLC analysis of plants revealed the highest accumulation of catechin and myricetin in the plants regenerated under RB light sources.

“Exotic Sensation” or “Völkisch Art”? Press Reviews of the Indisches Ballett Menaka (Menaka Indian Ballet) on Tour Through Germany, 1936–1938

The tour of the Indian Ballet Menaka through hundreds of German cities between 1936 and 1938 left a large footprint in the form of theatre reviews. This article focuses on the role of these performances in actualizing a specific knowledge about India that was, firstly, based on the assumption of the consanguinity of Indo-German peoples and, secondly, on a vision of history as a realization of the utopian ideal of cultural regeneration through art. This article thus hopes to articulate the ways in which the unique experience of this Indian theatre event served as an instrument for consolidating a völkisch/racialized perception of art in general, and of music in particular.

DIY Music and Contemporary Art Spaces: Centre for Contemporary Arts, Transmission and the Art-Music Nexus in Glasgow

ABSTRACT This article examines the relationship between contemporary art spaces and ‘Do-It-Yourself’ (DIY) music in Glasgow. It demonstrates the continuity and interconnectedness of DIY music and art practices by considering the transition of galleries from ‘white cubes’ to music venues and spaces for social interaction. Following a brief overview of Glasgow’s urban renewal and cultural regeneration, it traces the emergence of the Centre for Contemporary Arts (CCA) and Transmission and provides an outline of their organisational structure and policies. It then explores ethnographically how they have facilitated the cross-pollination between DIY music and contemporary art after the 2008 financial crisis. Approaching art galleries as social spaces that provide a platform for DIY musicians invites a holistic understanding of the reciprocal relationship between DIY music and contemporary art institutions in Glasgow. Therefore, the article considers in turn how these spaces were influenced by the ethics of DIY music. The malleability of contemporary art spaces and their relationship to the DIY ethos demonstrate that cultural policies should aim to strengthen the existing grassroots communities that form the bedrock of the city’s cultural scenes. This necessitates a move beyond the labels ‘post-industrial’ and ‘creative city’, thus decoupling cultural activities from the discourse of urban competition and Glasgow’s economic goals.

A comparative study of cultured and field plants provides evidence for the shy male hypothesis in tropical genotypes of Bryum argenteum Hedw.

ABSTRACT Introduction The female-biased sex ratio is recurrently observed in bryophyte populations, although the sex ratio of spores is usually balanced. The results of several studies have suggested explanations for this observation, one of which is the shy male hypothesis. The shy male hypothesis predicts that the sex ratio of populations is balanced in the field, but due to a low or lack of sex expression of male plants, the populations present a female bias when the sex ratio is based on observations of sex-expressing plants. Methods We tested the shy male hypothesis using samples of the moss Bryum argenteum collected from Brazilian seasonally dry tropical forests and the Brazilian Atlantic Forest. Field sex ratios were determined by classifying shoots as male, female, or non–sex-expressing. Non–sex-expressing shoots were further analysed for sex expression after culture regeneration. Results and discussion Our results showed that populations of Bryum argenteum in the field had a low rate of sex expression, and that most of the shoots expressed were female. However, when shoots without sex expression were cultured, a greater number of male shoots were observed compared with in the field plants. Because more males appeared in the shoots cultured from non–sex-expressing field plants, our data for tropical B. argenteum tend to support the shy male hypothesis. Some genotypes from the wet forest were synoicous (i.e. had male and female gametangia in the same gametoecia), suggesting polyploidy.

장소정체성 요소로서의 공공예술 활용이 문화마을의 경관요소에 미치는 영향에 관한 연구

In order to identify the role of public art and landscape planning in the Regional Cultural Area as part of the cultural regeneration of the local community, this study analyzed the placeness and sense of place of these regions, focusing on the cases of Korea, China and Japan. The first analysis qualitatively analyzed the history and locality of each region. The framework of the second quantitative analysis is a correlation analysis of the cultural regeneration strategy, the function of public art, and the concept of landscape elements, and uses adjectives of landscape status with a specific generation in China, that is, millennials in their 20s and 30s as parameters. A questionnaire was conducted. As a result of the 1st and 2nd analysis, it was analyzed that each case exhibited a place-linked, place-dependent, place-dominant public art piece, and had a sense of place with mediating, experiential, and symbolic shared values. Traditional decorative public art form was the main form, but partly participation and intervention, and new genre public art form were shown. The sense of place perceived by the subjects was normally distributed with an average median value of 3 or higher on a Likert 5-point scale, and the difference was not large. However, among the landscape elements, there was a difference in systematicity, continuity, convenience, and originality, and a cross-section of the subjects' unconscious cognitive process could be confirmed.

The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies.

Exhaustive analysis of genetically modified crops over multiple decades has increased societal confidence in the technology. New Plant Breeding Techniques are now emerging with improved precision and the ability to generate products containing no foreign DNA and mimic/replicate conventionally bred varieties. In the present study, metabolomic analysis was used to compare (i) tobacco genotypes with and without the CRISPR associated protein 9 (Cas9), (ii) tobacco lines with the edited and non-edited DE-ETIOLATED-1 gene without phenotype and (iii) leaf and fruit tissue from stable non-edited tomato progeny with and without the Cas9. In all cases, multivariate analysis based on the difference test using LC-HRMS/MS and GC-MS data indicated no significant difference in their metabolomes. The variations in metabolome composition that were evident could be associated with the processes of tissue culture regeneration and/or transformation (e.g. interaction with Agrobacterium). Metabolites responsible for the variance included quantitative changes of abundant, well characterised metabolites such as phenolics (e.g. chlorogenic acid) and several common sugars such as fructose. This study provides fundamental data on the characterisation of gene edited crops, that are important for the evaluation of the technology and its assessment. The approach also suggests that metabolomics could contribute to routine product-based analysis of crops/foods generated from New Plant Breeding approaches.

Response of Different Safflower Genotypes to Anther Culture

In this study; the responses of 12 safflower genotypes to anther culture were determined in MS medium containing increasing doses of BAP (0, 0.5, 1, and 2 mg L-1) and NAA (0, 0.1, and 0.5 mg L-1). Donor plants were grown under field conditions and the main capitulas were collected in early or late uninucleate stage. A pre-treatment was applied to their capitules at +4 °C for 3-4 days. Only cvs. Balcı and Linas produced regenerable callus among these genotypes. Although regenerable callus rate was higher in Balcı than Linas, shoot regeneration was achieved only in Linas with 0.5 or 1 mg L-1 of BAP + 0.1 mg L-1 of NAA combinations. In total, 8 shoots of Linas were moved to ½ MS medium consisting of 0.1 mg L-1 of NAA, but rooting of them could not be performed. In sum, among the 12 safflower genotypes, cvs. Balcı and Linas have higher anther culture regeneration potential; however, it is necessary to focus on shoot development and rooting on these cultivars in further experiments.

Postulating the impossible? The ideal of Gesamtkunstwerk in interwar avant-garde periodicals and their manifestos

The nineteenth-century principle of Gesamtkunstwerk was inseparably linked to the interwar avant-garde, as indicated in a plethora of manifestos and programmatic writings published in periodicals such as De Stijl, 7 Arts and Blok. The widely postulated interrelation of the arts in the service of social and cultural regeneration, however, did not always prove easy to implement. The social renewal of the Total Work of Art prophesied by avant-garde writers, painters and architects could hardly be achieved. This contribution seeks to answer the question whether the very synthesis of arts too was just an unachievable dream. Based on selected programmatic writings from avant-garde periodicals of Polish, Dutch and Belgian provenance I will analyse the recurring similarities and divergences that characterised various approaches to achieving the Total Work of Art voiced by particular artists. Subsequently I will scrutinise the chronicles of the actual cooperation between artists and architects in order to shed light on the actual application of the Gesamtkunstwerk ideal and the (im)possibility of its implementation.