Critical Control Point Approach Research Articles

Processing complementary foods to reduce mycotoxins in a medium scale Tanzanian mill: A hazard analysis critical control point (HACCP) approach

Designing and implementing processing procedures for producing safe complementary foods in dynamic and unregulated food systems where common food staples are frequently contaminated with mycotoxins is challenging. This paper presents lessons about minimizing aflatoxins (AF) in groundnut flour and AF and/or fumonisins (FUM) in maize and groundnut pre-blended flour for complementary feeding in the context of a dietary research intervention in rural Tanzania. The flours were processed in collaboration with Halisi Products Limited (Halisi), a medium scale enterprise with experience in milling cereal-based flours in Arusha, Tanzania. Using a hazard analysis critical control point (HACCP) approach for quality assurance, two critical control points (CCPs) for AF in processing the pre-blended flour were identified: 1) screening maize before procurement, and 2) blending during the processing of each constituent flour. Blending of maize flour was also identified as a CCP for FUM. Visual inspection during screening and sorting were identified as important control measures for reducing AF, but these steps did not meet the criteria for a CCP due to lack of objective measurement and verifiable standards for AF. The HACCP approach enabled the production of low AF (<5 μg/kg) and FUM (<2 μg/g) flours with low rejection rates for the final products. The paper presents practical lessons that could be of value to a range of commercial processors in similar low- and middle-income contexts who are keen on improving food quality.

Risk factors for food contamination among children discharged from community management of acute malnutritionprogrammes in South Sudan: A cross-sectional study and hazard analysis critical control pointapproach.

Children under-5 years of age are particularly vulnerable to severe acute malnutrition (SAM), and the risk factors associated with relapse to SAM are poorly understood. Possible causes are asymptomatic or symptomatic infection with enteric pathogens, with contaminated food as a critical transmission route. This cross-sectional study comprised a household survey with samples of child food (n = 382) and structured observations of food preparation (n = 197) among children aged 6-59 months that were discharged from treatment in community management of acute malnutrition (CMAM) programmes in South Sudan. We quantified Escherichia coli and total coliforms (TCs), measured in colony forming units per g of food (CFU/g), as indicators of microbial contamination of child food. A modified hazard analysis critical control point (HACCP) approach was utilised to determine critical control points (CCPs) followed by multivariate logistic regression analysis to understand the risk factors associated with contamination. Over 40% (n = 164) of samples were contaminated with E. coli (43% >0 E. coli CFU/g, 95%CI 38%-48%), and 90% (n = 343) had >10 TCs (CFU/g) (>10 TC CFU/g, 95%CI 87%-93%). Risk factors associated (p < 0.05) with child food contamination included if the child fed themselves (9.05 RR, 95% CI [3.18, 31.16]) and exposure to animals (2.63 RR, 95% CI [1.33, 5.34]). This study highlights the risk factors and potential control strategies that can support interventions that reduce food contamination exposure in young children and help further protect those that are highly vulnerable to recurrent exposure to enteric pathogens.

Probiotics isolated from the shubat in chicken meat production: importance and HACCP analysis

Probiotics are becoming more widely acknowledged as a safe and effective alternative to antibiotics for improving the safety of chicken meat. The investigation of potential hazards throughout the chicken meat supply chain enables a thorough assessment of contamination risks, allowing for the establishment of control and corrective actions within the corresponding processes. The ultimate goal is to ensure the safety of chicken meat for consumers. The objective of this study is to ascertain the potential risks that may arise inside the chicken production process, encompassing physical, chemical, and biological factors, with the identification of critical control points (CCPs). The study is also aiming to identify corrective strategies and approaches for decreasing the hazards associated with using of probiotics obtained from shubat. The research conducted involved conducting microbiological evaluations to test the ability of probiotics to withstand the presence of pathogenic bacteria. Additionally, the study examined the use of probiotics in chicken production, employing careful monitoring and comprehensive analyses to figure out the effectiveness of the supplements. For hazard identification and risk assessment, the Hazard Analysis and Critical Control Points (HACCP) approach was used, with the key findings showing that the most critical activities throughout the entire chain pertained to the use of probiotics as an alternative to antibiotics.

Integrated flood management based on Hazard Analysis Critical Control Point approach

AbstractThis study proposes to adopt the Hazard Analysis Critical Control Point (HACCP) approach and its seven principles to assist integrated flood management and transboundary cooperation in river basins, extending over more municipalities, regions, or states. Although HACCP was initially employed in the food production and processing industry to identify hazards and reduce risks throughout the production process, its principles have already been successfully applied to other sectors, such as water supply and wastewater treatment. The paper focuses (a) on the adaptation of the HACCP approach for integrated flood management, for example, for assessing the impact of planned upstream measures on downstream areas, and (b) on step 3 of the proposed Flood‐HACCP approach establishing critical limits for CCPs. For the demonstration purposes, the confluence of the Slovenian part of Drava River and its tributaries, Meža and Mislinja, is selected. Results indicate that keeping the Meža River discharge within the range of ±5% from its initial value would cause only minor local changes of flood situation within the selected case study area. On the other hand, variations to the Mislinja River discharge could change the flood situation to a greater extent and in a more diffuse way.

Seaweed aquaculture: a preliminary assessment of biosecurity measures for controlling the ice-ice syndrome and pest outbreaks of a Kappaphycus farm

The application of biosecurity in seaweed aquaculture plays an important role in reducing the impact of disease and pest outbreaks. The continuous occurrence of seaweed pests including the macroalgal epiphytes, epi-endophytic filamentous algae and biofilms on Kappaphycus farms may also potentially induce further incidences of the ice-ice syndrome. In this study, on-farm biosecurity management measures were tested on the commercially grown seaweeds Kappaphycus malesianus and Kappaphycus alvarezii during peak ice-ice season at Gallam-Gallam Village, Sabah, Malaysia. The investigation was focused on preventative control measures including the early detection of the ice-ice syndrome and pests through propagule health checks, regular cleaning of the crop thallus and associated long-line ropes and monitoring of the environment. Farm procedures and practices were also assessed in terms of their biosecurity ‘risk’ using the hazard analysis and critical control point (HCCAP) approach. Observations were replicated in two different farm management systems; one system adopted routine biosecurity measures and the other had no biosecurity measures. The results showed that the ice-ice syndrome and pest outbreak was significantly decreased by 60–75% for K. malesianus and 29–71% for K. alvarezii at the farm which adopted the routine biosecurity measures compared with the no biosecurity treatment. The biosecurity measures also significantly improved growth rate and seaweed quality. The infection levels of the epi-endophyte Melanothamnus sp. contributed to the ice-ice syndrome in K. malesianus, whilst the epiphyte coverage was correlated to the ice-ice incidence in K. alvarezii. This study provides the first evidence of biosecurity management measures significantly decreasing the incidence of the ice-ice syndrome and pests on a commercial seaweed farm.

Predictive water virology using regularized regression analyses for projecting virus inactivation efficiency in ozone disinfection

Wastewater reclamation and reuse have been practically applied to water-stressed regions, but waterborne pathogens remaining in insufficiently treated wastewater are of concern. Sanitation Safety Planning adopts the hazard analysis and critical control point (HACCP) approach to manage human health risks upon exposure to reclaimed wastewater. HACCP requires a predetermined reference value (critical limit: CL) at critical control points (CCPs), in which specific parameters are monitored and recorded in real time. A disinfection reactor of a wastewater treatment plant (WWTP) is regarded as a CCP, and one of the CCP parameters is the disinfection intensity (e.g., initial disinfectant concentration and contact time), which is proportional to the log reduction value (LRV) of waterborne pathogens. However, the achievable LRVs are not always stable because the disinfection intensity is affected by water quality parameters, which vary among WWTPs. In this study, we established models for projecting virus LRVs using ozone, in which water quality and operational parameters were used as explanatory variables. For the model construction, we used five machine learning algorithms and found that automatic relevance determination with interaction terms resulted in better prediction performances for norovirus and rotavirus LRVs. Poliovirus and coxsackievirus LRVs were predicted well by a Bayesian ridge with interaction terms and lasso with quadratic terms, respectively. The established models were relatively robust to predict LRV using new datasets that were out of the range of the training data used here, but it is important to collect LRV datasets further to make the models more predictable and flexible for newly obtained datasets. The modeling framework proposed here can help WWTP operators and risk assessors determine the appropriate CL to protect human health in wastewater reclamation and reuse.

Inactivation kinetics modeling of Escherichia coli in concentrated urine for implementing predictive environmental microbiology in sanitation safety planning

Urine concentration (condensation) leads to the inactivation of pathogens in urine owing to a hyperosmotic environment. This study proposed an inactivation kinetic model of Escherichia coli (E. coli), a surrogate of human bacterial pathogens, in concentrated synthetic urine. The model parameters were obtained under an assumption that the inactivation rate of E. coli followed a binomial distribution, which made it possible to accurately simulate the time-course decay of E. coli in synthetic urine. The inactivation rate constant values obtained in concentrated urine samples, ammonium buffer solutions and carbonate buffer solutions indicated that the osmotic pressure was a relatively predominant cause for the inactivation of E. coli. The appropriate storage time was estimated using the approach of quantitative microbial risk assessment, which indicated that the 5-fold concentrated urine could be safely collected after 1-day storage when urea was hydrolyzed, whereas 91-hour storage was required for non-concentrated urine. The occupational risk was not negligible even with 6-month storage at 20 °C when urea was not hydrolyzed, which suggested that the urine storage styles should be clarified more minutely. The present study highlights the importance of “predictive environmental microbiology,” which deals with inactivation kinetic models of microorganisms under varied environmental conditions to fully implement the hazard analysis and critical control point (HACCP) approach for the safe use of human excreta in agriculture.

Risk factors for child food contamination in low-income neighbourhoods of Maputo, Mozambique: An exploratory, cross-sectional study.

In low‐ and middle‐income countries, food may be a critical transmission route for pathogens causing childhood diarrhoea, but basic food hygiene is often overlooked in public health strategies. Characterising child food contamination and its risk factors could help prioritise interventions to reduce foodborne diarrhoeal disease, especially in low‐income urban areas where the diarrhoeal disease burden is often high. This cross‐sectional study comprised a caregiver questionnaire coupled with food sampling, and food preparation observations, among the study population of an ongoing sanitation trial in Maputo. The aim was to determine the prevalence of child food contamination and associated risk factors. The prevalence of Enterococcus spp., as an indicator of faecal contamination, was estimated in food samples. Risk factor analyses were performed through zero‐inflated negative binomial regression on colony counts. A modified hazard analysis and critical control point approach was used to determine critical control points (CCPs) that might effectively reduce risk. Fifty‐eight linked caregiver questionnaires and food samples were collected, and 59 food preparation observations were conducted. The prevalence of enterococci in child foods exceeding 10 colony forming units per gram was 53% (95% confidence interval [40%, 67%]). Risk factors for child food contamination were identified, including type of food, food preparation practices, and hygiene behaviours. CCPs included cooking/reheating of food and food storage and handling. This exploratory study highlights the need for more research into diarrhoeagenic pathogens and foodborne risks for children living in these challenging urban environments.

Parenteral Nutrition Preparation Procedure: A Hazard Analysis and Critical Control Points Approach at a Pediatric Hospital in Vietnam

Background: Hazard analysis and critical control points (HACCP) is a tool for food safety assurance, but the application of HACCP in healthcare facilities in Vietnam has been understudied and underregulated given a lack of governmental guidelines. This study aimed to explore the hazards and determine the critical control points related to the parenteral nutrition (PN) preparation procedures in a pediatric hospital in Vietnam. Methods: A cross-sectional study was conducted at Children Hospital No. 2 in Ho Chi Minh City, Vietnam, from July to September 2019. The study subjects were eleven standard operating procedures for preparation of a PN product called Vaminolact 6.5%. The hazards were explored based on the probability of occurrence, the severity of consequence, and the quantity of influenced individuals. Each step in the PN procedure was determined by multiplying the points of three standards. A step with a point equal to or higher than 18 was considered a hazard. The critical control point (CCP) was determined by following the five-question decision tree. Results: Eight hazards were explored via hazard analysis, including sanitation prior to compounding, measurement of basal solution, compounding, labeling, sanitation of preparation room, in-out control, air flow control, and primary filter system. Nine critical control points were determined as follows: sterilization by alcohol, glove change per every 10 vials, basal solution measurement, compounding, mini-spike change per every 10 vials, syringe change per every 10 vials, air flow control, primary filter system, and sterilization check-up. Conclusion: The hospital manager should consider this study as support for an evidence-based adjustment to improve PN preparation procedure.

Development of a Robust Canine Welfare Assessment Protocol for Use in Dog (Canis Familiaris) Catch-Neuter-Return (CNR) Programmes.

Simple SummaryThis paper describes the development of a robust composite welfare assessment tool to evaluate the welfare of free-roaming dogs passing through surgical sterilisation or catch-neuter-return (CNR) programmes. Catch-Neuter-Return programmes are frequently employed by governments and animal welfare charities to control the rising population of free-roaming dogs. Due to the focus on dog population control, individual dog welfare may be compromised through the CNR process, which comprises necessary stressors and harms, including capture, transport, surgery, and social disruption. Using a combination of a modified Delphi analysis and a Hazard Identification and a Critical Control Point analysis, this project described potential hazards to dog welfare during the CNR process and identified critical points where dog welfare could be assessed, and mitigations put in place to improve dog welfare throughout the CNR process. This project resulted in the development of a composite dog welfare assessment tool that will allow the robust assessment of the welfare of free-roaming dogs in CNR programmes and allow CNR projects to benchmark and mitigate hazards to dog welfare.The aim of this study was to develop a welfare assessment tool based on objective, reliable and relevant measures to be applied to individual dogs as they underwent a Catch-Neuter-Return (CNR) programme. A modified Delphi method and Hazard Analysis and Critical Control Points (HACCP) approach was used to develop the composite canine welfare assessment protocol, comprising both animal-based and resource-based measures. This draft welfare assessment protocol was then trialed and refined in existing CNR programmes to identify key control points where individual dog welfare may be moderately or significantly compromised in the CNR process. The results show that animal-based welfare indicators, e.g., pain behaviours, which provide a more direct indication of an animal’s welfare state, require training and skill to recognise, whilst resource-based indicators are simple to measure but act only as indirect measures of welfare. We concluded that whilst CNR projects can potentially improve the health and welfare of free-roaming dogs in the long-term, the risk of short-term welfare harms during the CNR process is high. Thus, it is essential for staff involved in dog population management programmes to assess the welfare state of dogs in CNR and take remedial action to safeguard individual dog welfare.

A critical control point approach to the removal of chemicals of concern from water for reuse

The reuse of water in a range of potable and non-potable applications is an important factor in the augmentation of water supply and in improving water security and productivity worldwide. A key hindrance to the reuse of water is the cost of compliance testing and process validation associated with ensuring that pathogen and chemicals in the feedwater are removed to a level that ensures no acute or chronic health and/or environmental effects. The critical control point (CCP) approach is well established and widely adopted by water utilities to provide an operational and risk management framework for the removal of pathogens in the treatment system. The application of a CCP approach to barriers in a treatment system for the removal of chemicals is presented. The application exemplar is to a small community wastewater treatment system that aims to produce potable quality water from a secondary treated wastewater effluent, however, the concepts presented are generic. The example used seven treatment barriers, five of which were designed and operated as CCP barriers for pathogens. The work demonstrates a method and risk management framework by which three of the seven barriers could also include a CCP approach for the removal of chemicals. Analogous to a CCP approach for pathogens, the potential is to reduce the use of chemical analysis as a routine determinant of performance criteria. The operational deployment of a CCP approach for chemicals was augmented with the development of a decision tree encompassing the classification of chemicals and the total removal credits across the treatment train in terms of the mechanistic removal of chemicals for each barrier. Validation of the approach is shown for an activated sludge, ozone and reverse osmosis barrier.

Development of a food safety toolkit for dry common beans (Phaseolus vulgaris L.) in Uganda using a Hazard Analysis and Critical Control Point (HACCP) approach

Common beans ( Phaseolus vulgaris L) may be contaminated with heavy metals and aflatoxins. Cooked beans may also be contaminated with micro-organisms due to poor hygiene and sanitation practices. Hazard Analysis and Critical Control Point (HACCP), which is a globally recognised food safety program, was proposed as a suitable program to minimise/eliminate the risk of contamination. Therefore, the objective of this study was to develop a HACCP plan for dry common beans in Uganda and an accompanying food safety toolkit. The seven principles of HACCP as outlined by Codex Alimentarius were followed to develop a HACCP plan for the dry common beans value chain in Uganda. A decision tree diagram was further used to identify each potential hazard at each processing stage and Critical Control Points (CCPs) along the chain. The identification of the CCPs was further supported by an evaluation of the actual risk and severity of the hazard. For the CCP identified, reliable control mechanism and corrective actions were established to fulfill the requirements set by the critical limits to guarantee the safety of the products. Verification and records systems were proposed to determine the effectiveness and traceability of the HACCP plan. For identified CCPs, a co-creation methodology was used to develop the food safety toolkit. This was carried out in four sessions that included a background of the chain actors’ ambitions to determine the suitability of the toolkit, assessment of CCPs, expert advice on the CCP and an exercise to develop concepts for each CCP. From the analysis, fourteen processing stages starting from land selection to cooking and serving were identified. Out of these, four stages were CCPs. These were land selection and preparation, storage, post-harvest drying, and cooking and serving. Hazards at the CCPs included heavy metals, mycotoxins, and micro-organisms such as S. aureus, E. coli, and Salmonella spp. A combination of good hygiene and sanitation practices and good agricultural practices were recommended as control measures against the hazards. To further equip the value chain actors with mitigation strategies, a food safety toolkit whose usefulness is to give the actors a systematic means to control identified CCPs was developed. In this regard, the toolkit and HACCP plan will complement each other. From the study results, implementation of the toolkit, followed by an assessment of its uptake and impact on livelihoods and food safety risks is recommended. Key words: Common beans, food safety toolkit, prerequisite programs, HACCP plan, hazards, Uganda

Four decades of water recycling in Atlantis (Western Cape, South Africa): Past, present and future

The primary aquifer at Atlantis (Western Cape, South Africa) is ideally suited for water supply and the indirect recycling of urban stormwater runoff and treated domestic wastewater for potable purposes. The relatively thin, sloping aquifer requires careful management of the artificial recharge and abstraction for balancing water levels. Water quality management is a further key issue at Atlantis for ensuring the highest quality potable water. Groundwater quality varies from point to point in the aquifer, while urban runoff and wastewater qualities vary greatly. The layout of the town allows for the separation of stormwater from the residential and industrial areas as well as separate treatment of domestic and industrial wastewater. This permits safe artificial recharge of the various water quality portions at different points in the aquifer, either for recycling or for preventing seawater intrusion. All of the management actions are dependent on detailed data collection and this paper describes the various parts of the system, describes the data collection activities, and provides results of the monitoring and aquifer responses over the past four decades. Challenges related to iron fouling of production boreholes are also described. The presence of emerging contaminants was studied in 2008 but requires follow-up research for establishing the extent of any possible threat to water recycling. In order to address the shortcomings of the system a risk management plan based on the Hazard Analysis and Critical Control Points approach was developed. Lessons learnt from the Atlantis experience can be transferred to other potential sites for establishment of similar systems in arid and semi-arid areas of South Africa and the African continent. Keywords: Atlantis, managed aquifer recharge, water recycling, groundwater, stormwater, wastewater, monitoring

Recommendations for dealing with waste contaminated with Ebola virus: a Hazard Analysis of Critical Control Points approach.

ObjectiveTo assess, within communities experiencing Ebola virus outbreaks, the risks associated with the disposal of human waste and to generate recommendations for mitigating such risks.MethodsA team with expertise in the Hazard Analysis of Critical Control Points framework identified waste products from the care of individuals with Ebola virus disease and constructed, tested and confirmed flow diagrams showing the creation of such products. After listing potential hazards associated with each step in each flow diagram, the team conducted a hazard analysis, determined critical control points and made recommendations to mitigate the transmission risks at each control point.FindingsThe collection, transportation, cleaning and shared use of blood-soiled fomites and the shared use of latrines contaminated with blood or bloodied faeces appeared to be associated with particularly high levels of risk of Ebola virus transmission. More moderate levels of risk were associated with the collection and transportation of material contaminated with bodily fluids other than blood, shared use of latrines soiled with such fluids, the cleaning and shared use of fomites soiled with such fluids, and the contamination of the environment during the collection and transportation of blood-contaminated waste.ConclusionThe risk of the waste-related transmission of Ebola virus could be reduced by the use of full personal protective equipment, appropriate hand hygiene and an appropriate disinfectant after careful cleaning. Use of the Hazard Analysis of Critical Control Points framework could facilitate rapid responses to outbreaks of emerging infectious disease.

Process control in phytosanitary irradiation of fresh fruits and vegetables as a model for other phytosanitary treatment processes

Phytosanitary treatments reduce the risk of commodities carrying invasive quarantine pests to negligible levels. Ionizing radiation processing of fresh fruits and vegetables to prevent successful infestation from these pests is steadily growing in commercial use because it provides safe solutions to quarantines and is tolerated by more fresh produce than any alternative treatment in use. Unlike all other commercial phytosanitary treatments (heat, cold, fumigation), the burden of proof of efficacy of phytosanitary irradiation is entirely on process control. Facilities for phytosanitary irradiation processing are more tightly controlled than facilities for other treatments, and the process control involved can serve as a model to harmonize and streamline the latter. Basing treatment certifications of efficacy on process control instead of inspection will help guide phytosanitation towards a phytosanitary hazard analysis and critical control point approach more consistent with other forms of food process control.

Recommendations for dealing with waste contaminated with Ebola virus: a Hazard Analysis of Critical Control Points approach

Recommendations for dealing with waste contaminated with Ebola virus: a Hazard Analysis of Critical Control Points approach

Sensitivity Analysis for Critical Control Points Determination and Uncertainty Analysis to Link FSO and Process Criteria: Application to Listeria monocytogenes in Soft Cheese Made from Pasteurized Milk

Microbiological food safety is an important economic and health issue in the context of globalization and presents food business operators with new challenges in providing safe foods. The hazard analysis and critical control point approach involve identifying the main steps in food processing and the physical and chemical parameters that have an impact on the safety of foods. In the risk-based approach, as defined in the Codex Alimentarius, controlling these parameters in such a way that the final products meet a food safety objective (FSO), fixed by the competent authorities, is a big challenge and of great interest to the food business operators. Process risk models, issued from the quantitative microbiological risk assessment framework, provide useful tools in this respect. We propose a methodology, called multivariate factor mapping (MFM), for establishing a link between process parameters and compliance with a FSO. For a stochastic and dynamic process risk model of Listeriamonocytogenes in soft cheese made from pasteurized milk with many uncertain inputs, multivariate sensitivity analysis and MFM are combined to (i) identify the critical control points (CCPs) for L.monocytogenes throughout the food chain and (ii) compute the critical limits of the most influential process parameters, located at the CCPs, with regard to the specific process implemented in the model. Due to certain forms of interaction among parameters, the results show some new possibilities for the management of microbiological hazards when a FSO is specified.

A Systems Approach for Management of Pests and Pathogens of Nursery Crops.

Horticultural nurseries are heterogeneous and spatially complex agricultural systems, which present formidable challenges to management of diseases and pests. Moreover, nursery plants shipped interstate and internationally can serve as important vectors for pathogens and pests that threaten both agriculture and forestry. Current regulatory strategies to prevent this movement of pathogens and pests with nursery plants are based on visual inspections of plants just before shipping, a process that is costly and inadequate. Here we propose the application of a systems approach for horticultural nurseries modeled after the Hazard Analysis of Critical Control Points (HACCP) approach widely used in the food processing industry. We evaluated aspects of the systems approach to analyze contamination hazards by Phytophthora species and then implemented management practices targeting specific critical control points. The systems approach for analyzing and correcting unsafe practices offers a potential alternative strategy for preventing plant contamination that could be broadly applied to many pests and pathogens. One of the hallmarks of this approach is its flexibility, including the ability to improve and adapt the approaches as new technologies are developed or improved and to provide the grower with management options.

Quality related principles of the South African beef classification system in relation to grading and classification systems of the world

This paper addresses the principles related to different grading and classification systems of the world with specific focus on beef quality related outcomes. The paper uses the definitions that classification is a set of descriptive terms describing features of the carcass that are useful as guidelines to those involved in the production, trading and consumption of carcasses, whereas grading is the placing of different values on carcasses for pricing purposes, depending on the market and requirements of traders and consumers. The literature shows that the criteria used in grading systems rank carcasses fairly accurately according to expected eating experience of the loin muscles but not of higher connective tissue cuts of the hind and fore quarter. Criteria used in classification systems give limited descriptions of the quality related characteristics of the carcass. Only the Meat Standards Australia (MSA) cuts based grading system of Australia seeks to define or predict consumer satisfaction with a cooked meal for each cut of the carcass. Its success is based on a palatability assured critical control point (PACCP) approach to satisfy the consumer. However, MSA requires high technical skills, a well organised infrastructure and proper traceability, high level of integrity from different role players and could be very costly, involving high additional personnel cost. The South African classification system should probably focus on distinguishing between young feedlot and somewhat more mature pasture animals with different criteria within each sub category to describe the variation in product quality. Correct pre-slaughter and slaughter management (stress, weight, chilling rate, electrical stimulation, post mortem aging) could improve consistency within age group.

Effects of Temperature Abuse on the Survival, Growth, and Inactivation ofSalmonella typhimuriumin Goat Milk

The growth and survival of Salmonella typhimurium in goat milk samples at different shifting temperatures were evaluated. The growth of S. typhimurium at lower temperatures (5°C, 10°C, and 15°C) exhibited bacteriostatic effects in milk, whereas at ambient temperature (25°C) and at 45°C, this pathogen luxuriantly grew throughout the 12-h stationary phase. At 50°C this pathogen was found to be thermotolerant and could still thrive in the milk. Overall, shifting temperatures from 37°C to 55°C and 60°C clearly indicated S. typhimurium to have reached complete elimination. The results demonstrated that the adaptation and survival of this pathogen directly depend on temperature stress. It is expected that the results will be useful to dairy industries for implementation of good manufacturing practices with a better hazard analysis critical control point approach to predict the microbial risk assessment and also benefit the consumers.