You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology II1 Apr 2017MP19-19 PATIENTS WITH CALCIUM STONES AND RANDALL’S PLAQUE EXCRETE DISTINCT POPULATIONS OF MICRO RNA-CONTAINING URINARY EXTRACELLULAR VESICLES John Lieske, Xiangling Wang, Robin Chirackal, John Knoedler, Amy Krambeck, Felicity Enders, Andrew Rule, Pritha Chanana, and Muthuvel Jayachandran John LieskeJohn Lieske More articles by this author , Xiangling WangXiangling Wang More articles by this author , Robin ChirackalRobin Chirackal More articles by this author , John KnoedlerJohn Knoedler More articles by this author , Amy KrambeckAmy Krambeck More articles by this author , Felicity EndersFelicity Enders More articles by this author , Andrew RuleAndrew Rule More articles by this author , Pritha ChananaPritha Chanana More articles by this author , and Muthuvel JayachandranMuthuvel Jayachandran More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.3261AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Randall’s plaques (RP) appear to be an important precursor of urinary stone disease. However, RP cannot be noninvasively detected. Furthermore, the biologic processes that mediate growth of RP remain unclear. This study was designed to identify specific micro RNA (miRNAs) changes within urinary extracellular vesicles (EVs) based on stone forming status, and the amount of intrarenal RP. METHODS A total of 40 subjects were included in this study. First time (incident) stone formers and population controls were recruited from the community (n=10 each). RP were assessed via endoscopic digital imaging with quantitative analysis in consecutive idiopathic calcium oxalate stone formers undergoing percutaneous surgery for stone removal. Subjects with high amounts of RP (HP; > 5% papillary surface area coverage; n=10) were age (+/- 5 yrs) and sex matched to a group with low amounts of RP (LP; < 5% papillary surface area coverage; n=10). Small non-coding miRNAs within urinary EVs were quantitated by XRNA Exosome RNA-Seq Library Kit (System Biosciences, Palo Alto, CA). Differentially expressed miRNAs with a p-value of 0.05 or lower were chosen for pathway analysis and miRNA target prediction comparing LP versus HP, and population controls versus stone formers. RESULTS When controls were compared to stone formers, a total of 10 miRNA were increased (6 to 10-fold), while 5 miRNA were decreased (2 to 5-fold). When LP were compared to HP stone formers, 3 miRNA were increased (6.5 to 10-fold) while 7 miRNA were decreased (6-9 fold). The upregulated miRNAs contribute in calcification, cell proliferation, acute kidney injury, renal fibrosis, pro-apoptotic and pro-inflammatory pathways whereas down-regulated miRNAs contribute anti-apoptotic and anti-inflammatory processes, prevent renal fibrosis, ischemic injury, and progression of chronic kidney disease. CONCLUSIONS Stone formers and those with high amounts of RP excrete distinct populations of miRNAs within urinary EVs. These miRNAs may serve as novel biomarkers to indicate the presence of RP. These miRNAs may also provide new insights into early renal cellular processes in the progression of stone pathogenesis and RP and new tools for the screening, diagnosis, risk stratification and monitoring of pharmacological therapy for persons with idiopathic stone disease. Further studies to validate and extend these observations are needed. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e238 Advertisement Copyright & Permissions© 2017MetricsAuthor Information John Lieske More articles by this author Xiangling Wang More articles by this author Robin Chirackal More articles by this author John Knoedler More articles by this author Amy Krambeck More articles by this author Felicity Enders More articles by this author Andrew Rule More articles by this author Pritha Chanana More articles by this author Muthuvel Jayachandran More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...