Cotton Quality Research Articles

Identification of quantitative trait loci for fiber quality, yield, and plant height traits in Upland cotton

AbstractIn cotton, most agronomic traits are controlled by polygenes. In this study, 110 F6 recombinant inbred lines (RILs), derived from Upland cotton cross NC05AZ06 x NC11‐2100, were used to develop a linkage map and to identify quantitative trait loci (QTL) for six fiber quality traits, three yield traits, and plant height. These RILs were genotyped using the CottonSNP63K array and phenotyped for fiber quality, yield traits, and plant height in 2‐year field trials. Analysis of variance revealed significant (p < 0.05) differences among RILs for all traits studied, and the heritability estimates were moderate (30%–60%) to high (> 60%). Both positive and negative correlations were observed for fiber quality and yield traits. A total of 3,774 polymorphic SNP markers were used to develop a genetic map with an average marker density of 1.54 SNP/cM. Thirty QTL for fiber quality traits, yield traits, and plant height were detected on 15 different chromosomes, explaining 6.80%–20.02% of the phenotypic variance (PVE). Of these, 14 were major QTL (PVE > 10%), and three major QTL were detected in both years. Candidate gene analysis in the major QTL detected in both years and plant height QTL with PVE of 20.02% revealed five putative genes for fiber quality traits and one putative gene for plant height. The linkage map and identified QTL along with the candidate genes in the study could serve as additional breeding resources for Upland cotton genetic improvement.

Comparative transcriptome analysis of interspecific CSSLs reveals candidate genes and pathways involved in verticillium wilt resistance in cotton (Gossypium hirsutum L.)

Verticillium wilt (VW), known as the ‘cancer’ of cotton, is a vascular disease of cotton caused by Verticillium dahliae, which seriously affects the yield and quality of cotton. Upland cotton (Gossypium hirsutum, Gh) has good yield but low resistance to VW, while Sea-island cotton (G. barbadense, Gb) has excellent resistance to VW and good fiber quality but low yield. Creating chromosome segment substitution lines (CSSLs) from progeny of interspecific cross between Gh and Gb is a way to combine the superior traits of Gh and Gb to breed cotton varieties with high yield and fiber quality as well as good disease resistance. In this study, a CSSL population derived from Emian22 (Gh) × 3–79 (Gb) was subject to disease assay in multiple environments to identify VW-resistant CSSLs. The two CSSLs (M20 and M34) showing stable VW-resistance in different assay conditions together with the VW susceptible parent Emian22 were selected for comparative transcriptome analysis to identify genes associated with cotton response to V. dahliae infection. Significant transcriptome changes were found to occur at each time point (24, 48, and 72 h) after V. dahliae infection in all three lines. Gene ontology (GO) analysis of the DEGs identified in each line revealed enriched terms shared by the three lines or unique to each line. Based on gene expression trend analysis, the DEGs identified in M20, M34, and Emian22 were clustered into 8, 8, and 7 cluster, respectively, with one unique to Emian22 and one unique to both M20 and M34. Weighted gene co-expression network analysis (WGCNA) identified 8 modules significantly related to cotton response to V. dahliae infection, especially the cyan module with 22 hub genes, including two genes (Ghi_D02G10111 and Ghi_A03G10581) encoding probable protein phosphatase 2. Suppressing the expression level of Ghi_D02G10111 (GhPP2C52) by virus-induced gene silencing led to the reduction of VW-resistance, implying GhPP2C52 being a positive regulator of VW-resistance. In addition, genes of the ABA and Ca2+ signaling pathways were found to be associated with the response of cotton plants to V. dahliae infection. The findings of the study revealed the molecular difference between the VW-resistant and VW-susceptible cotton lines. Combining the genetic mapping results of the same population, the results of this study will facilitate cloning and molecular characterization of the genes responsible for VW-resistance observed in M20 and M34 and finally accelerate breeding VW-resistant cotton varieties through marker assisted selection and gene editing.

Phenomics and transcriptomics analyses reveal deposition of suberin and lignin in the short fiber cell walls produced from a wild cotton species and two mutants.

Fiber length is one of the major properties determining the quality and commercial value of cotton. To understand the mechanisms regulating fiber length, genetic variations of cotton species and mutants producing short fibers have been compared with cultivated cottons generating long and normal fibers. However, their phenomic variation other than fiber length has not been well characterized. Therefore, we compared physical and chemical properties of the short fibers with the long fibers. Fiber characteristics were compared in two sets: 1) wild diploid Gossypium raimondii Ulbrich (short fibers) with cultivated diploid G. arboreum L and tetraploid G. hirsutum L. (long fibers); 2) G. hirsutum short fiber mutants, Ligon-lintless 1 (Li1) and 2 (Li2) with their near isogenic line (NIL), DP-5690 (long fibers). Chemical analyses showed that the short fibers commonly consisted of greater non-cellulosic components, including lignin and suberin, than the long fibers. Transcriptomic analyses also identified up-regulation of the genes related to suberin and lignin biosynthesis in the short fibers. Our results may provide insight on how high levels of suberin and lignin in cell walls can affect cotton fiber length. The approaches combining phenomic and transcriptomic analyses of multiple sets of cotton fibers sharing a common phenotype would facilitate identifying genes and common pathways that significantly influence cotton fiber properties.

Fine mapping and candidate gene analysis of qFL-A12-5: a fiber length-related QTL introgressed from Gossypium barbadense into Gossypium hirsutum.

The fiber length-related qFL-A12-5 identified in CSSLs introgressed from Gossypium barbadense into Gossypium hirsutum was fine-mapped to an 18.8 kb region on chromosome A12, leading to the identification of the GhTPR gene as a potential regulator of cotton fiber length. Fiber length is a key determinant of fiber quality in cotton, and it is a key target of artificial selection for breeding and domestication. Although many fiber length-related quantitative trait loci have been identified, there are few reports on their fine mapping or candidate gene validation, thus hampering efforts to understand the mechanistic basis of cotton fiber development. Our previous study identified the qFL-A12-5 associated with superior fiber quality on chromosome A12 in the chromosome segment substitution line (CSSL) MBI7747 (BC4F3:5). A single segment substitution line (CSSL-106) screened from BC6F2 was backcrossed to construct a larger segregation population with its recurrent parent CCRI45, thus enabling the fine mapping of 2852 BC7F2 individuals using denser simple sequence repeat markers to narrow the qFL-A12-5 to an 18.8 kb region of the genome, in which six annotated genes were identified in Gossypium hirsutum. Quantitative real-time PCR and comparative analyses led to the identification of GH_A12G2192 (GhTPR) encoding a tetratricopeptide repeat-like superfamily protein as a promising candidate gene for qFL-A12-5. A comparative analysis of the protein-coding regions of GhTPR among Hai1, MBI7747, and CCRI45 revealed two non-synonymous mutations. The overexpression of GhTPR resulted in longer roots in Arabidopsis, suggesting that GhTPR may regulate cotton fiber development. These results provide a foundation for future efforts to improve cotton fiber length.

Investigation of local resistance and air velocity in narrowing pipes for the transport of fibrous materials

In the world, one of the main tasks is to improve the quality of cotton and products from it, the introduction of energy and resource-saving technologies and technical means at enterprises, which make it possible to reduce their cost. In the priorities of the Decree of the President of the Republic of Uzbekistan dated January 28, 2022 No. PF-60 “New Development Strategy of the Republic of Uzbekistan for 2022-2026”, the third priority area called “Advanced development of the national economy and ensuring high growth rates” clarifies the inclusion of a number of tasks in the state program. Air pipes are used in most stages of the primary cotton processing process. In this direction, a scientific base is being created to improve the efficiency of the process of transporting fibrous materials by air, special attention is paid to improving product quality and reducing costs through the creation of automated pneumatic transport systems, and the widespread introduction of modern pneumatic equipment and technology into production is accelerating. Although many studies have been done on the loss of fibrous products during the air transport of cotton and cotton products, some fibrous material is released into the atmosphere. To prevent this, it is necessary to determine the aerodynamic resistances that form in the areas of the changed surface of pneumatic transport pipes, and develop recommendations for their reduction. In this article, based on the results of theoretical and practical studies, local resistances are determined in the zones of narrowing of air pipelines of pneumatic transport, and the change in air speed is presented in graphical form.

The effects and interrelationships of intercropping on Cotton Verticillium wilt and soil microbial communities

BackgroundCotton Verticillium wilt, causing by Verticillium dahliae, has seriously affected the yield and quality of cotton. The incidence of Verticillium wilt in cotton fields has been on the rise for many years, especially after straw has been returned to the fields. Intercropping can reduce the incidence of soil borne diseases and is often used to control crop diseases, but the relationship between the effects of intercropping on microbial communities and the occurrence of plant diseases is unclear. This research explored the relationship between soil microbial community structure and Cotton Verticillium wilt in interplanting of cotton-onion, cotton-garlic, cotton-wheat and cotton monocultures. Amplicon sequencing applied to the profile of bacterial and fungal communities.ResultsThe results showed that the disease index of Cotton Verticillium wilt was significantly reduced after intercropping with cotton-garlic and cotton-onion. Chao1 and Sobs indices were not significantly different in the rhizosphere soil and pre-plant soils of the four planting patterns, but the pre-plant fungal shannon index was significantly lower in the cotton-onion intercropping plot than in the other three plots. PCoA analysis showed that the soil microbial communities changed to a certain extent after intercropping, with large differences in the microbial communities under different cropping patterns. The abundance of Chaetomium was highest in the cotton-garlic intercropping before planting; the abundance of Penicillium was significantly higher in the cotton-wheat intercropping than in the other three systems.ConclusionCotton-garlic and cotton-onion interplanting can control Cotton Verticillium wilt by affecting the soil microbial community. Fungi of the genera Chaetomium and Penicillium may be associated with plant disease resistance.

Analysis of the main effect clustering and decision-making coefficients for F2 generation of upland cotton in Southern Xinjiang

Yield and fibre quality traits respectively accounting for 4 and 5 of their 278 varieties (lines) and their 784 F2 crosses of upland cotton were for their additive and dominance effects by a genetic model comprising additive, dominance and their interaction effects with the environment in two years of Alar, South Xinjiang, People’s Republic of China. Based on the additive and dominance effects, all varieties were clustered using cluster analysis of the R software package.Then, the decision-making coefficient of F2 was analyzed. Results indicated that under the high-density planting mode of "low plant growth (plant height: 0.8–1.0 m), high density (225,000–300,000/hm−2), early ripe and film cover" in Southern Xinjiang Province, the additive effects of 278 parents were divided into fourteen groups. The average additive effects of yield and fibre quality traits of 21 varieties (lines) in the fourth group were at a good level. Obtain offspring was easy with both yield (except Lint percentage) and fibre quality traits by crossing between these varieties (lines). The fifth group had a better average additive effect on fibre quality traits, whilst the tenth group had a higher average additive effect on yield traits. The progeny with complementary yield and fibre quality traits could be obtained by crossing these two kinds of varieties. The dominance effects of 278 parents were divided into thirteen groups. The fourth group included 33 varieties (lines) that yield traits, and fibre quality traits (except lint percentage)were all at a better average level in dominance effect, which could be used as parents for hybrid utilisation of both yield traits and fibre quality traits. The average dominance effect of yield traits was higher in the first group, and the average value of dominance effect of fibre quality traits in the twelfth group was high. Results indicated the order of significant relationships of dominance effect, the decision-making traits of lint percentage to lint yield, length and micronaire. The decision-making traits of strength was length. The main decisive traits and restricted traits for improving the yield and fibre quality of upland cotton were also determined.

Exogenous Application of Chitosan Mediated Biochemical, Phenological, Quality, and Yield Attributes of Heat-stressed Cotton (Gossypium hirsutum L.)

Exogenous Application of Chitosan Mediated Biochemical, Phenological, Quality, and Yield Attributes of Heat-stressed Cotton (Gossypium hirsutum L.)

Effect of deep softeners on irrigation, salt washing and cotton yield on soils whose mechanical composition is heavy and meliorative status is difficult

In the article, studies were conducted on the economy of quality saline washing and irrigation water of saline soils by deepening medium coarse soils with a mechanical content of melioration, plaster, salinity in the conditions of Bukhara region. In experiments, basically, there were three ways to soften the field: plowing in the first experiments at a depth of 35 cm, plowing in the second experiment at 60 cm, and plowing in the third experiments at a depth of 80 cm. During the experiments, in the first variant of the studies, the norm of salt washing was set to 6480 cbm/ha, in the second variant to 5210 cbm/ha and in the third variant to 5010 cbm/ha. First studies in irrigation of Bukhara included 102 varieties, that is, in the control variant to 5821 cbm/ha, in the second variant to 5284 cbm/ha and in the third variant to 5100 cbm/ha. They were watered 5 times during the season. At the same time, high quality cotton was harvested due to the increase in the cotton yield to 0.39 t/ha in variant 2 and 0.129 t/ha in variant 3 compared to the control. And the land reclamation situation improved. The amount of chlorine ion in the active layer is 0.003 in variant 2 compared to the control. The amount of dry residue decreased by 0.058% in variant 2 and the amount of dry residue by 0.063% in variant 3.

Development and performance evaluation of picking mechanism for knapsack cotton picker

One of the most significant fibre cash crops in both India and the rest of the world is cotton (Gossypium herbaceum). India is the world’s second-largest cotton producer. Cotton is hand-picked by human labours in India, which is a laborious and time-consuming task. In existing knapsack cotton picker, the cotton quality was compromised due to the impact of the impeller vanes during conveying of picked cotton to collector. Keeping all these aspects in mind, a picking mechanism for knapsack power driven cotton picker was developed with considering the agronomical parameters, functional requirements, engineering requirements and ergonomic aspects. As a power source, a 0.9 kW, 5500 rpm petrol engine was used. The remaining components of the knapsack cotton picker, such as the aspirator, impeller, sucking assembly, collecting sack, mounting frame and so on, were developed according to design specifications. It was evaluated how effectively the knapsack cotton picker performed in terms of picking capacity, picking efficiency, harvesting losses, trash content, the quality of machine-picked cotton, and operation costs. Based on trial results using cotton varieties as GPG-3, a knapsack cotton picker’s picking capacity was determined to be 7.62 kg/ h. The average picking efficiency for cotton picking using a knapsack cotton picker was 95.79%. The harvesting losses and trash content were determined as 4.21% and 8.13%, respectively in case of knapsack cotton picker. The fiber quality in terms of span length, uniformity ratio and strength was not affected by knapsack cotton picker. There was reduction in time requirement and the picking cost by 51.44 and 14.86%, respectively over manual picking method. Cotton picking with a knapsack cotton picker showed a 3.27% increase in overall net realization over manual picking.

NAC domain transcription factor gene GhNAC3 confers drought tolerance in plants

Abiotic stress seriously affects the growth, yield, and fiber quality of cotton. It is of great importance to cultivate drought-resistant and salt-tolerant cotton. NAC (NAM, ATAF1/2, and CUC2) is a plant-specific transcription factor, which is widely involved in the response to abiotic stress. Here, we discovered the GhNAC3 gene isolated from the expression profile of drought stress in cotton and verified its functions in cotton. First, GhNAC3 was strongly induced expression by drought and salt stresses. Gene structure analysis revealed that GhNAC3 had a conserved NAC domain and was homologous to several stress-related NAC transcription factors gene of Arabidopsis. Subcellular localization and transcriptional activation assays revealed that GhNAC3 was a nuclear protein with a C-terminal transcriptional activation domain. Overexpression of GhNAC3 enhanced Arabidopsis tolerance to drought stress with reduced sensitivity to ABA, characterized by increased germination and cotyledon rates under drought stress, and promoted root elongation. VIGS silencing of GhNAC3 reduced cotton tolerance to drought stress as indicated by the low water content of the leaves under drought treatment, significantly faster water loss and lower ABA content in detached leaves, along with the accumulation of more hydrogen peroxide (H2O2) and malondialdehyde (MDA). In conclusion, GhNAC3 plays an important role in the abiotic stress of cotton, which might have great application potential in molecular breeding of cotton varieties with drought resistance.

Predicting cotton fiber properties from fiber length parameters measured by dual-beard fibrograph

Cotton fiber properties, although strongly influenced by plant growth conditions, are largely dictated by the cotton variety; therefore, certain inherent associations exist among these properties. Previous studies examined the mutual influences of cotton properties (e.g., fiber maturity on strength), but latent associations between fiber length and other important properties (e.g., fineness, maturity and strength) have not been explored. This paper attempted to investigate these relationships, and to create regression models to predict the fiber properties from the length parameters so that an overview on cotton quality can be provided when only length measurements are available. We collected 100 cotton samples as a training set and 17 extra samples as a testing set, and measured the fiber length parameters using the dual beard fibrograph and the seven other fiber properties (strength, elongation, micronaire, nep, fineness, immature fiber content, and maturity ratio) using the High Volume Instrument and Advanced Fiber Information System. We then performed the correlations, multicollinearity, regression and clustering analyses on the fiber properties. It was found that the fiber length parameters had moderate associations (0.3<|r|<0.7) with the seven properties, and the prediction errors for the training set varied from 2.25% (maturity ratio) to 14.36% (nep). The Bland–Altman analysis proved that for all the seven properties, more than 94.9% of the predicted and actual points were within the 95% agreement limits and without systematic biases. The regression models based on the five cotton clusters consistently lowered the prediction errors through the optimally aggregated fiber properties. The comparable results were obtained from the testing set, which demonstrated the good generalization power of the prediction models.

Effect of Different Levels of Organic Matter and Genotypes on Seed Yield and Fiber Quality in Cotton (Gossypium hirsutum L)

Effect of Different Levels of Organic Matter and Genotypes on Seed Yield and Fiber Quality in Cotton (Gossypium hirsutum L)

Estimating gene action and combining ability for yield and fiber quality in cotton (Gossypium hirsutum L.)

Genetic evaluation of segregating populations is one of the primary activities of cotton breeders. Seven cotton genotypes were crossed in a line ? tester mating design. The population of 12 F1, 12 F2 and their parents were tested in Randomized Complete Block Design with three replications at the Nazilli Cotton Research Institute of T?rkiye during the 2014/2015 cotton-growing season. The genetic variability was significant for all characters studied. ?2GCA/?2SCA indicated non-additive gene action in controlling seed cotton yield (F2), ginning out-turn (F1) and fiber quality traits for both generations. The highly favourable GCA effects for seed cotton yield and fiber strength were recorded in the Carmen cultivar. Carmen ? Carisma hybrid exhibited high mean performance and SCA effects for seed cotton yield and fiber strength, while the performance of Gloria ? ST-468 was superior in terms of ginning out-turn. We concluded that individual plants with desired traits should be selected in later generations due to the preponderance of non-additive gene actions.

The Effect of the Application of Biodux Stimulator in Growing High and Quality Cotton From Cotton

In the article, the scientific basis of the new stimulators on enzyme activity, biosynthesis of amino acids and proteins, and the physiological processes that occur during cotton development, the effect of new stimulators on seed and cotton during its growth period, on seed germination, cotton growth and development, and resistance to gommosis and wilt diseases, were determined. . Biodux stimulator provides 8.512.1% higher field fertility of seed, and the highest result was achieved at the rate of 3.0 ml/t. Also, when cotton was treated with Biodux at the rate of 2.0 ml/ha during the flowering period, the plant height was 7.7 cm higher, the yield branches were 1.3 pieces and the number of bolls was 1.8 more, and the degree of opening of bolls was 7.7-13.7 % has been found to be accelerated.

Effect of “Bist” and “Bist-M” biopreparations on quality and productivity of wheat and cotton yields in agro-ecological zones of Uzbekistan

Significant emphasis is placed on the development and application of biopreparations harnessed from beneficial bacteria, which exhibit advantageous traits for plants. A multitude of experiments have substantiated that the concurrent utilization of biopreparations in conjunction with mineral fertilizers opens avenues to cultivate high-quality agricultural produce. Researchers across diverse regions have demonstrated that not only can plant productivity be augmented through this approach, but also the attainment of superior, environmentally-friendly yields is feasible, stemming from an exploration of the intricate interplay between plants and microorganisms. This growing body of scientific evidence underscores the potential of leveraging the symbiotic relationship between plants and beneficial microorganisms. This synergy holds the promise of enhancing agricultural output while championing the production of products that meet stringent ecological standards. As such, the collaborative influence of biopreparations and traditional agricultural practices stands as a noteworthy avenue for fostering sustainable and quality-driven agricultural endeavors.

A Model for Detecting Fungal Diseases in Cotton Cultivation using Segmentation and Machine Learning Approaches

This research detailed a model for detecting fungal diseases via techniques for processing images of cotton leaves. The work allowed to develop a model based on the set of preprocessed data, to formulate the developed model, to simulate and evaluate the model. It is about detecting fungal diseases in cotton cultivation. The image data records were collected in an online data repository consisting of images of cotton leaves infected with fungal diseases and normal leaf images. In addition, other images of infected and uninfected cotton leaves were collected in cotton production fields in the Ségbana region in Benin Republic. The model was formulated based on watershed segmentation technique by applying Edge Detection algorithm and K-Means Clustering; and Support Vector Machine (SVM) for classification. The simulation was done using MATLAB with Image Processing Toolbox 9.4. The results gave an accuracy of 99.05%, specificity 90%, misclassification rate 0.95%, recall rate 99.5% and precision 99.5%. In addition, with less computational effort and in less than a minute, the best results were obtained, showing the efficiency of the image processing technique for the detection and classification of infected and uninfected leaves. It was concluded that this approach was applied to detect fungal diseases on cotton leaves to promote the production and harvest of good quality cotton and valuable cotton products.

Development of the mineral fertilizer application system for cotton yards under different soil-climatic conditions of Uzbekistan

Today, in order to develop cotton production in Uzbekistan, all attention is paid to the use of modern, promising ways of farming, in particular, to free up cotton fields for other crops and to increase the gross yield by planting cotton varieties with high fiber yield and high quality in relatively small areas, in each province and farm in the region where cotton is grown. - breeding of varieties suited to climatic conditions is being focused on. The cultivation of high and quality cotton depends primarily on the yield potential of cotton varieties and the compliance of cotton fibers with standard requirements. Therefore, creating new cotton varieties and placing the most promising ones in accordance with the different soil and climatic conditions of Uzbekistan remains an urgent task of scientists and specialists. Care of different cotton varieties, taking into account the fact that their requirements for nutrients, water, soil and climatic conditions and other agrotechnical factors are different depending on their biological characteristics, it is considered very necessary to develop specific recommendations and put them into practice.

Effect of using repeated and siderate crops on soil fertility, yield of cotton varieties, and technological quality indicators of cotton fiber in short rotation cropping systems

In the 1:1 (cotton:grain) system of short-rotation, soybean, bean, mung bean crops were harvested as a repeat crop after winter wheat. Their stem crop and mixed siderate crops (perco, oats, green peas) were planted in the second half of August, and the blue mass crop grown in October-November was harvested for livestock. The lower 15-20 cm part of the stem was plowed under the plow as a blue manure, at the end of one rotation, the amount of organic carbon in the soil increased by 0.041-0.073%, and the total nitrogen content by 0.012-0.016% compared to the initial values. The highest rate of dry mass accumulation of cotton was observed when using short-row rotation 1:1, winter wheat + mixed siderate crops (perco, oat, green pea):cotton system. The dry mass of the leaves of the Navroz variety of cotton was 22.6 g, the dry mass of the stem was 33.4 g, the dry mass of the bolls was 18.5 g, and the dry mass of the cotton was 48.0 g. The total dry mass of 1 plant was equal to 122.5 g. In the 1:1 (cotton:grain) system of short-rotation, when cotton was grown as a repeat crop after legume-cereal (soybean, mung bean) and mixed siderate (perco, oat, green pea) crops, it provided a higher and better quality cotton yield. Cotton yield was 3.2-5.8 tons/ha, fiber output was 1.0-1.5 percent, and 1000 seed mass was 9.0-11.0 g/ha. Besides, the length of the fiber was found to be higher by 1.4-2.0 mm.

EFFECT OF SAW GINNING ON THE FIBRE QUALITY OF Bt AND NON-Bt COTTON

Bt cotton refers to a plant which has Bacillus thuringiensis (Bt) toxins in many of its cells. This naturally occurring soil bacterium will be used to reduce insect damage from bollworm, pink bollworm, and budworm. Therefore, farmers who are cultivating the Bt cotton variety will not be subjected to spray pesticides to control these worms. Whereas in this study, the non-Bt cotton (DP-90) refers to the commercially known variety which have no Bacillus thuringiensis (Bt) toxins in its cells. During the cultivation of non-Bt cotton varieties, the control of bollworms is done through the application of pesticides, which is a costly exercise in terms of cost of pesticides, spray equipment and labour. Along with the cost of cultivation, the best index to cotton quality is the performance of the fibres during spinning at the textile mill. In the present study the effect of saw ginning to the quality of both varieties was studied. The result could help the spinners to predict the preperformance of both varieties when subjected to the mechanical action of modern high rotating spinning machines parts. Ginning results a significant effect (at 0.05 level of significance) in all other measured fibre quality properties (upper half mean length, length uniformity index, short fibre content by number and by weight, level of neps, single fibre tenacity and elongation) of both Bt and non-Bt (DP-90) cotton varieties. The impact of ginning on the studied fibre quality properties was relatively severer on Bt cotton varieties than non-Bt (DP-90) varieties.