Corneal Epithelium Research Articles (Page 1)

Introduction Acanthamoeba is a ubiquitous protozoan pathogen that can cause a severe ocular infection, Acanthamoeba keratitis. Despite its high prevalence and potential contamination of contact lenses, the natural behavior of this parasite remains poorly understood. Therefore, we investigated Acanthamoeba trophozoite movement, rate of encystment, trophozoite size, and phylogenetic relationships between eight prevalent Acanthamoeba genotypes. Methods Acanthamoeba was seeded onto a plate with and without E. coli . After initial size measurements were recorded, images were taken using a microscope to create time-lapse videos over a 72-hour period. Amoeba trophozoite tracks were quantified for distance, displacement, and speed. Separately, Acanthamoeba cysts were generated naturally over the course of the study via nutrient deprivation in ¼ Ringer’s over 72 hours. Wells were stained with calcofluor white to identify cysts and wells were quantified for rate of encystment and cyst size. Results Of the eight genotypes investigated, T7 and T18 possessed the largest trophozoite size while T5 was the smallest. T5 was consistently the fastest genotype over the 72-hour period in both the fed and starved conditions. Nutrient conditions did not show any consistent impact on the overall distance, speed, or encystment of any genotype within 72 hours. Finally, while some genotypes (T1, T11) demonstrated a relatively high percentage of encystment at the 24-, 48-, and 72-hour timepoints, the other genotypes demonstrated a relatively low encystment percentage at these same times. Discussion Overall, these results indicate that eight of the common genotypes of Acanthamoeba vary widely in terms of size, speed, rates of encystment, and response to nutritional state. From these, we can infer that Acanthamoeba keratitis prevention methods must be robust enough to counter amoeba in trophozoite or cyst form, and that amoeba should be expected to be able to traverse a wide variety of distances (for instance, across a contact lens or onto a corneal epithelium) in either a fed or starved nutritional state.

Sox9 is a transcription factor with multiple roles during development and in adult organ homeostasis. In the adult eye, Sox9 expression persists in several cell types, including the retinal pigmented epithelium cells and the Müller glial (MG) cells, as well as in the limbal and corneal basal epithelia. To uncover the role of Sox9 in these cell types, we induced the deletion of the gene in adult mice. We found that, after Sox9 ablation, mutant mice undergo a severe process of retinal degeneration characterized by the loss of MG cells and complete depletion of the photoreceptors layer. Moreover, by combining single-cell RNA sequencing and Sox9 lineage tracing, we found that Sox9 is expressed in a basal limbal stem cell population with the ability to form two types of long-lived cell clones involved in stem cell maintenance and homeostasis. Mosaic analysis of Sox9 positive and negative cells confirmed that the gene is essential for limbal stem cell differentiation. Our results show that Sox9 is required for the maintenance of retinal integrity and for limbal stem cell differentiation in the adult mouse eye.

Myeloma overexpressed gene (MYEOV) was initially identified as a gene amplified in several malignancies and was found to promote cell proliferation and metastasis. Our previous comparative RNA sequencing and epigenetic analyses revealed high MYEOV levels in differentiated corneal epithelial cells and showed that TET2 epigenetically regulated MYEOV expression. In the current study, we aimed to further characterize the expression and regulation of MYEOV in the human ocular surface. MYEOV expression was examined by immunostaining of the human corneas and by analysis of the publicly available single-cell RNA sequencing data. Gene knockdown (KD) of MYEOV and the regulators of corneal epithelial differentiation, PAX6 and KLF4, in in vitro-expanded corneal epithelial cells was performed using siRNA transfection. Protein expression levels were examined by western blot. MYEOV KD cells were subjected to colony-forming and EdU cell proliferation assays and RNA sequencing analysis. Human cornea immunostaining revealed strong MYEOV expression in the KRT12-positive differentiated corneal epithelial cells, whereas the majority of KRT13-positive differentiated conjunctival epithelial cells were MYEOV negative. MYEOV expression was not detected in the other surface ectoderm-derived epithelia, such as skin and oral mucosa. Both PAX6 KD and KLF4 KD resulted in a reduction of MYEOV and KRT12 protein expression. MYEOV KD decreased cell proliferation. Our study revealed specific high MYEOV expression in KRT12-positive corneal epithelial cells among surface ectoderm-derived epithelia. Similar to KRT12, MYEOV expression is regulated by PAX6 and KLF4. Functionally, MYEOV regulates the proliferation of transient amplifying cells in the cornea.

This study aimed to investigate the therapeutic effects of pasireotide (SOM230) on corneal nerve abnormalities in a mouse model of dry eye disease (DED) and to elucidate its potential mechanisms of action. Male C57BL/6J mice underwent lacrimal gland excision (LGE) to induce DED and were treated with SOM230 or PBS. Corneal epithelial integrity, pain-related behaviors, and corneal sensitivity were evaluated. RNA sequencing was performed to identify potential pathways modulated by SOM230. Flow cytometry and immunofluorescence quantified macrophage polarization states. SOM230 treatment significantly improved corneal epithelial integrity, reduced pain behaviors, and restored corneal sensitivity compared to PBS. RNA sequencing revealed that SOM230 downregulated key inflammatory pathways, including TNF-α and NF-κB. Flow cytometry and immunofluorescence showed that SOM230 modulated macrophage polarization by decreasing pro-inflammatory M1 macrophages and enhancing anti-inflammatory M2 macrophages, contributing to reduced corneal inflammation and improved healing. SOM230 alleviates nerve abnormalities in DED by suppressing inflammatory pathways (TNF-α, NF-κB) and promoting a shift in macrophage polarization from the pro-inflammatory M1 phenotype to the reparative M2 phenotype. These findings suggest that modulation of macrophage polarization is a crucial mechanism underlying the therapeutic effects of SOM230, highlighting its potential as a treatment strategy for nerve damage in DED.

Silencing USP10 alleviates persistent corneal epithelial defect in mice.

Single-cell RNA sequencing reveals LGALS9+ epithelial and COMP+ stromal cell crosstalk driving keratoconus pathogenesis.

There is no molecular evidence available confirming or contradicting inter-eye molecular variability in keratoconus (KTCN). The key question is whether the use of a model of more and less affected eyes of the same pair facilitates the identification of specific molecular features of KTCN severity. This retrospective case-control study involved 21 KTCN patients (n = 132 experimental samples derived from 42 corneal epithelium samples separated into central, middle, and peripheral topographic regions) analyzed in the paired model. Transcriptomic (RNA-sequencing) and proteomic (MALDI-TOF/TOF MS/MS) profiling was performed. An additional non-paired model, including KTCN patients (n = 42) and controls (n = 14), strengthened the assessment. Then, key findings were validated in a rediscovery study with 20 patients using reverse-transcription quantitative polymerase chain reaction, immunofluorescence staining, and confocal microscopy. In the paired model, which included patients with ≥1 grade difference in topographic keratoconus classification, 48 differentially expressed genes were identified. Over-representation analysis highlighted the GO term "cell-cell adhesion" (adjusted P = 0.028), with key contributors including ACTN1, EPCAM, PCDH19, PVR, and TFRC. TFRC showed significantly higher expression in the middle topographic region of more severely affected eyes (P = 0.008, paired t-test), correlating with the average topographic region thickness (R = 0.53, P = 0.008) and flat keratometry (K1; R = 0.49, P = 0.016). Immunofluorescence confirmed intra-individual and regional differences in transferrin receptor (TFRC) protein expression, with increased expression in the middle topographic region of more advanced eyes, compared to eyes with forme fruste KTCN. Inter-eye variability implicates TFRC as a component of the KTCN severity signature, thus adding the element to the mechanisms underlying KTCN cone formation.

Corneal transplantation is often considered the last resort for severe corneal epithelial disorders, especially limbal stem cell deficiency (LSCD). Tissue engineering offers novel strategies to mitigate the shortage of corneal transplant donors. However, low cell viability and compromised functionality in tissue engineering represent a major challenge. In this review, we describe the key characteristics required for corneal epithelium bioscaffolds. We summarize the research progress centered on optimizing cell activity and functionality in the past 10 years from four key perspectives: the sourcing of cells, seed cell pretreatments, biomaterial optimization, and engineered culture system innovation. The sources, isolation, and induction methods of seed cells are described, and the advantages and disadvantages of existing clinical treatment methods are compared. Furthermore, we compare existing clinical therapies and summarize promising seed cell pretreatment strategies for the first time. Several innovative engineered cell culture systems are exhibited as well. We demonstrated how to preserve cell viability through bioscaffold stiffness modulation, topographic design, and application of innovative fabrication techniques. Finally, we propose a personalized and precise regeneration strategy based on high-resolution images, digital modeling, bioprinting, and machine learning.

This study analyzed the outcomes of corneal epithelium reconstruction using the method of paralimbal oral mucosal epithelial transplantation (PLOMET) in patients with bilateral limbal stem cell deficiency. PLOMET surgery was performed in 10 patients with bilateral limbal stem cell deficiency. Surgical outcomes were monitored for 12 months postoperatively. Impression cytology of the ocular surface with immunohistochemical (IHC) analysis for specific keratins (K3, K4, K7, K12) was performed before and after surgery in addition to standard and supplementary diagnostic methods. Corneal re-epithelialization within 1-2 months was achieved in all patients. In the late postoperative period, 2 out of 10 patients experienced spontaneous epithelial erosions, associated with systemic comorbidities and certain environmental factors. At 12 months postoperatively, 9 out of 10 patients demonstrated slight improvement in visual acuity, and best-corrected visual acuity exceeded 0.1 in 2 out of 10 patients. Grading of corneal status by three independent experts demonstrated a reduction in conjunctivalization, decreased neovascularization, and improved corneal transparency in 8 out of 10 patients. IHC results showed positive staining for K4, characteristic of oral mucosal epithelial cells, in 7 out of 8 patients, whereas only one patient exhibited marked K12 expression characteristic of corneal epithelium. Paralimbal oral mucosa epithelial transplantation (PLOMET) enables stable corneal re-epithelialization and partial improvement in visual acuity. The latter is limited by the degree of corneal stromal opacification, providing a rationale for optical keratoplasty as a second stage of visual rehabilitation in this patient population.

Corneal neovascularization (CNV) is one of the major causes of vision impairment and blindness worldwide. Efficient intrastromal drug delivery of antiangiogenic agents is a significant challenge due to the presence of various ocular barriers (i.e., tear barrier, corneal epithelial barrier, etc.). Here, we report a rapidly dissolving bilayer microneedle (MN) patch, combining the anti-VEGF antibody (Ava) with an integrin-targeted anti-inflammatory drug (PF-Y-RGD), achieving synergistic therapy for CNV. Equipped with microscale needle tips, the topically applied MN patches reversibly pierce the corneal epithelium to generate microchannels, bypassing the tear/epithelial barrier and facilitating the transport of payload drugs into the stromal layer of the cornea, which significantly enhances drug bioavailability. Such MN patches exhibit biphasic drug release behavior and are also readily applicable and minimally invasive to impart good ocular tolerance. A rabbit model of CNV reveals that the combination of Ava with PF-Y-RGD in such bilayer MN patches robustly inhibits neovascularization and reduces neovascular areas and the expression of various cytokines (i.e., VEGF, TNF-α, IL-1β, MMP-9) in the cornea. As a strategy for efficient intrastromal drug delivery, this study promises an easy and effective way to treat CNV, which may also lead to approaches for curing various ocular disorders.

PurposeDiabetic keratopathy, a common ocular complication of diabetes, is characterized predominantly by corneal epithelial damage and peripheral nerve injury. This study examined the role of adiponectin (ADPN) in regulating the repair of the diabetic corneal epithelium and accompanying nerve injuries.MethodsRNA sequencing was performed on total RNA isolated from corneal epithelium of streptozotocin (STZ)-induced type 1 diabetic mice and type 2 diabetic BKS.Cg-Dock7m +/+ Leprdb/Nju (db/db) mice to identify differentially regulated pathways and interactions. ADPN receptor expression was assessed. Recombinant ADPN, ADPN receptor 1/2 siRNA, and a phosphorylated AKT (p-AKT) inhibitor were then utilized in diabetic mice and in human corneal epithelial cells (HCECs) cultured under high-glucose conditions to evaluate corneal wound healing responses.ResultsADPN receptor expression and p-AKT levels were downregulated in corneas of diabetic mice and in HCECs exposed to high glucose. Treatment with recombinant ADPN accelerated repair of corneal epithelial and nerve damage in both type 1 and type 2 diabetic mice, enhanced HCEC proliferation and migration under high-glucose conditions and activated AKT signaling. ADPN treatment also reduced neutrophil infiltration and inflammatory factor expression during wound repair. These beneficial effects were abolished by ADPN receptor 1 knockdown or AKT inhibition.ConclusionsOur results demonstrate that ADPN promotes the corneal epithelium and nerve regeneration in diabetic mice via activation of the AdipoR1/AKT signaling axis and suppression of inflammatory responses. These findings identify ADPN as a promising therapeutic candidate for promoting corneal epithelial wound healing in diabetic conditions.Supplementary InformationThe online version contains supplementary material available at 10.1186/s40662-025-00458-w.

Purpose: Ambroxol is a high-potency inhibitor of voltage-gated sodium channels Nav1.7 and Nav1.8 responsible for nociceptive and neuropathic pain. This study investigated the analgesic property of ambroxol on corneal pain in rats as well as its toxicity on human corneal epithelial cells. Methods: Inhibition of corneal sensation (CS) by ambroxol hydrochloride (AH) in Sprague-Dawley rats was assessed by Cochet-Bonnet esthesiometer. Rat corneal pain was induced by administering 5 M NaCl drops to the eyes, and eye wiping rates were recorded to reflect pain intensity. Subsequently, a rat neuropathic corneal pain (NCP) model was created by first removing the corneal epithelium and then provoking pain with 2 M NaCl. The analgesic property of 0.5% AH eye drop was assessed in rats by reductions in eye wiping rates. In cultured human corneal epithelial cells, the adverse effects of AH on cell migration and viability were studied by cell scratch and Cell Counting Kit-8 tests. Results: It was noted that 0.5% AH could significantly inhibit CS, and 0.5% AH is efficacious in suppressing rat corneal pain as well as heightened nociceptor sensitivity from repetitive stimulation from irritants. At concentrations up to 30 μM, AH does not affect corneal epithelial cell migration, cell proliferation, and viability. Conclusions: Selective blockage of key nociceptors of the cornea by ambroxol is effective in suppressing corneal pain and heightened nociceptor excitability, leading to modulation of NCP. Sparing certain CS could translate into more protection to the ocular surface. No overt toxicity of ambroxol was seen in cultured human corneal epithelial cells in this study.

"Evaluation of Crystalline Lens Equatorial Plane: A Novel SS-OCT Biometry Parameter For Predicting Postoperative Intraocular Lens Position".

The eye is one of the primary structures of the body that allows perception of the entire world. A person's activities rely entirely on having good vision, and any diseases or problems encountered with vision create a troublesome condition in life. Ocular delivery can potentially treat numerous eye-related disease conditions. The diseases that affect the eyes include glaucoma, dry eye syndrome, cataracts, conjunctivitis, diabetic retinopathy, keratitis, uveitis, Endophthalmitis, allergies, and others. The conventional dosage forms (eye drops, ointment) pose numerous challenges in treating ocular infections owing to their complex nature and several barriers. Recent advances in artificial intelligence and machine learning provide a preliminary diagnosis in the early stages of disease identification and are also useful during retinal surgery. The poor ocular penetration, low bioavailability, short retention time, and frequent administration are the limitations of conventional treatments. These limitations are easily solved with nanotechnology-driven approaches. The current state-of-the-art review explores eye physiology, barriers (precorneal, corneal epithelium, lacrimal sac, blood-ocular, and efflux protein), limitations of the conventional and nanotechnology-based delivery (Lipid-based, polymer-based, metal and inorganic NPs, vesicle-based NPs, and miscellaneous). These nanocarriers facilitate good permeation, extended retention time, augment solubility, improve bioavailability, improve patient comfort and compliance, and minimize dosage application. The nanocarriers are equally effective in treating the anterior and posterior regions of the eyes, whereas conventional ones have failed to treat them effectively. The recently approved agents and patents are elaborated on ocular drug delivery. Advancements in stem cell and gene therapy are also gaining attention for treating inherited and acquired retinal diseases.

Background/objectives: The continuous necessity to support biostrumental data with biolomecular data collected using non-invasive tools is influencing the world of ocular surface devices. The ocular imprint still represents a non-invasive and safety technique for collecting corneal and conjunctival epithelia in an easy way, as performed in human and veterinary clinics. Although used in clinical practice since 1977, operators might benefit from improvements in these techniques, especially in terms of handling and management. Methods: Herein, by reporting the design and characteristics of a patent of ocular surface sampling (the SurfAL pen and periocular-assisted SurfAL pen; PCT WO2016IB51474 20160316), we performed a validation and analysis of its value compared to gold standards. The level-headedness and advantages of this device were verified in 15 sclerocorneal specimens (sampling advantages) and tested in 25 volunteers (handling and operator efficiency, as well as frequency of discomfort in volunteers). Morphological as well as biomolecular analyses were used to compare SurfAL devices with conventional ones. Results: The easy management of SurfAL pens and the good detection of epithelial/goblet cells were confirmed. The SurfAL pen was found to be smart and suitable for routine analysis, as confirmed by quick and reproducible onsite sampling. Periocular-assisted SurfAL pen was comparable in terms of sampling quality but less comparable in terms of subject confidence due to its geometry. Conclusions: This study suggests that the SurfAL pen and periocular-assisted SurfAL pen might represent an additional and hands-on way of sampling ocular surface cells and improve the diagnostic route in ophthalmology.

The corneal barrier restricts the entry of xenobiotics (including drugs) in the anterior segment of the eye, resulting in less than 5% of the drug reaching the target site (deep corneal tissues) - necessitating frequent topical eye drop administration. Interestingly, despite these barriers, endogenous peptides are shuttled across the cornea by peptide transporters expressed on the corneal epithelium. The current study investigates the potential of targeting peptide transporters to increase the permeability of poorly permeable drugs across the cornea. D-α-tocopherol polyethylene glycol succinate (TPGS), an amphiphilic polymer, was conjugated with Glycyl-Sarcosine (Gly-Sar), a well-known peptide transporter ligand to develop nanomicelles. Natamycin, a poorly water-soluble and poorly permeable drug, was entrapped (0.6%) in Gly-Sar-TPGS-formulated nanomicelles. Both ex vivo and in vivo studies demonstrated that the natamycin-loaded Gly-Sar-TPGS nanomicelles (PeNatcel) enhanced permeation across the cornea. The PeNatcel demonstrated therapeutic efficiency against Candida albicans and Aspergillus flavus using in vitro and ex vivo antifungal studies. PeNatcel showed comparable activity against Candida albicans at 4 times a day dosing frequency compared to 12 times a day marketed 5% natamycin suspension. Consequently, the developed peptide transporter-specific ligand-conjugated nanomicelles delivery system can also be explored further for other water-insoluble and poorly permeable drugs.

A corneal abrasion results from the disruption or loss of cells in the corneal epithelium. If inadequately treated, it can compromise visual clarity. The wound healing process of a corneal abrasion involves epithelial migration, proliferation and adhesion. Clitoria ternatea flower extract (CTE) is rich in flavonoids, anthocyanins and other bioactive compounds. It has antioxidant, anti-inflammatory and wound-healing properties. This study explores the potential of CTE to be used as a natural supplement to improve corneal wound healing. Phytochemical profiling via LC–MS identified a total of 51 distinct bioactive constituents. The anthocyanin content, quantified in terms of cyanidin-3-glucoside equivalent, was quantified at 33.06 mg per gram of extract. The extract exhibited 33.8% DPPH radical scavenging activity and a total polyphenol content equivalent to 24.14 mg/g gallic acid. Human telomerase-immortalized corneal epithelial (hTCEpi) cells maintained in keratinocyte basal medium were utilized to determine cytotoxicity and wound-healing effects. The optimal extract concentration of 0.08 mg/mL, quantified via MTT assay, resulting in enhanced cell viability. Scratch assays demonstrated a higher percentage of wound closure in the CTE-treated group at 6 and 12 h relative to the untreated group, with statistical significance (p < 0.05). The gene expressions of CK3 and Cx43, quantified via qRT-PCR, showed no significant differences between groups. However, within the CTE-treated group, CK3 expression increased at 12 h relative to 0 h and 6 h, and Cx43 expression rose significantly at 12 h compared with 0 h (p < 0.05). Immunofluorescence confirmed positive protein expression of both markers. These findings suggest that CTE possesses potent antioxidant properties and promotes corneal epithelial wound healing through upregulation of CK3 and Cx43 in vitro.

Hyperglycemia threatens vision via inducing irreversible morphologic and physiologic changes in the corneal epithelium. We aimed to investigate whether glucosamine (GlcN) is capable of countering the detrimental effects of high glucose (HG) levels on human corneal epithelial (HCE-T) cells. GlcN failed to attenuate the HG-associated cytotoxicity in HCE-T cells owing to its inherent cytotoxicity. Compared with the decrease in the pAKT/AKT and p-p38/p38 ratios, GlcN induced the KLF4 (Krüppel-like factor 4) and SIRT1 (Sirtuin-1) proteins. Furthermore, GlcN mitigated hyperglycemic-induced reactive oxygen species (ROS) and cellular senescence, and elevated the population of cells in the subG1 and S phases but reduced the population in the G1 phase. Hyperglycemia-induced KLF4 proteins and SIRT1 and fibronectin proteins were enhanced and suppressed by 10 mM GlcN, respectively. GlcN induced the expression of the tight junction protein claudin-1, which was otherwise suppressed in hyperglycemic conditions. Lastly, two SIRT1 inhibitors Ex-527 and INZ mitigated GlcN-induced claudin-1 expression and suppression of hyperglycemic-induced ROS generation. Our study may provide valuable insights into developing therapeutic strategies that utilize GlcN to rejuvenate the corneal epithelium in patients experiencing oxidative and senescent stresses due to poor glycemic control.Supplementary InformationThe online version contains supplementary material available at 10.1038/s41598-025-19766-1.

HighlightsWhat are the main findings?Modern high-resolution anterior segment tomographers are capable of extracting surface height data from the corneal front and back surfaces and from the epithelium–stroma interface.The higher refractive index of the corneal epithelium suggests that the cornea should be considered as a dual-layer structure to account for potential inhomogeneity in the epithelial thickness.What is the implication of the main finding?Model surfaces, such as floating best-fit spheres or conoids, could be fitted to the height map data within a specific region of interest to determine relevant surface characteristics such as curvatures, asphericities, and apex positions.Based on a dataset with bilateral repeat measurements in a cataractous population, we were able to confirm that the extracted surface characteristics seem to be very robust. However, surface asphericity should be extracted from a larger region of interest to ensure more robust data.We investigated the repeatability of the MS-39 in determining power vector components—the spherical equivalent (SEQ) and astigmatic powers (C0 and C45) and asphericity (Q)—of corneal epithelium, stroma, and endothelium in a large patient cohort. In this retrospective cross-sectional single-centre study, we evaluated a dataset containing 600 MS-39 anterior segment tomography measurements from 200 eyes (three repeat measurements each) taken prior to cataract surgery. The exported measurements included height map data for the epithelium, stroma, and endothelium surface. Model surfaces (spherocylinder (SphCyl), cylindrical conoid (CylConoid), and biconic (Biconic), all in the 3/6 mm zone) were fitted using nonlinear iterative optimisation, minimising the height difference between the measurement and model. The mean (MEAN) and standard deviation (SD) for each sequence of measurements were derived and analysed. In the 3 mm and 6 mm zone, the MEAN SEQ was 53.47/53.56/53.57 and 53.21/53.54/53.54 D for SphCyl/CylConoid/Biconic for the epithelium, −4.47/−4.51/−4.51 and −4.45/−4.50/−4.50 D for the stroma, and −6.23/−6.26/−6.26 and −6.18/−6.29/−6.30 D for the endothelium. With the three surface models and the 3/6 mm zone, the SD for SEQ/C0/C45 was in the range of 0.04 to 0.11/0.05 to 0.13/0.04 to 0.11 D for epithelium; 0.01 to 0.02/0.01 to 0.05/0.01 to 0.06 D for stroma; and 0.01 to 0.02/0.02 to 0.07/0.03 to 0.07 D for endothelium. Fitting floating model surfaces with astigmatism to map data of the corneal epithelium, stroma, and endothelium seems to be a robust and reliable method for extracting equivalent power and astigmatism using all the datapoints within a region of interest.

Abstract Serving as the cornea's outermost barrier, the corneal epithelium is susceptible to persistent damage, which can lead to irreversible vision loss and severe neuropathic pain. Electrical stimulation bandage contact lenses (BCLs) can provide wound protection while accelerating the healing process. However, their opacity and complex design hinder their clinical adoption. This study proposes a bioactive BCL using poly(vinylidene fluoride‐co‐trifluoroethylene) (P(VDF‐TrFE)) through a simple fabrication process, which exhibits outstanding transparency and the ability to generate a uniform microelectric field over the injury site, while also offering superior smoothness, mechanical, and electrical properties. In vivo and in vitro experiments confirmed the excellent biocompatibility and effectiveness of P(VDF‐TrFE) BCLs in corneal epithelial wound healing, with RNA‐sequencing revealing the underlying mechanisms associated with corneal injury healing, such as cytoskeletal reorganization and inflammation regulation. Furthermore, the reorganization of the cytoskeleton and pseudopodia, which enhances the cellular ability to sense the injury environment and to promote migration and proliferation, is validated in co‐cultured human corneal epithelial cells. Additionally, P(VDF‐TrFE) BCLs inhibit excessive inflammation during the injury process, promoting a favorable healing environment. These findings position P(VDF‐TrFE) as a promising treatment option for corneal injuries, highlighting the broader potential of ferroelectric polymers in ophthalmic tissue engineering.