Stable isotope analysis has been extensively used to establish trophic relationships within avian communities of marine ecosystems. The Neotropic Cormorant (Phalacrocorax brasilianus) is a good representative of marine bird communities along the northern coasts of South America. The diet and trophic position of the Neotropic Cormorant during the non-breeding season in a tropical lagoon, Bocaripo-Chacopata, in northeastern Venezuela, was determined by using gut content analyses and stable isotope ratios of carbon (δ13C) and nitrogen (δ15N), from birds, fish and invertebrates muscle tissue. Gut content and stable isotopes both indicated that cormorants are strict piscivores, whose diet is mainly based on benthonic fish, and thus occupy a high trophic level, above that of the piscivorous fish in the same habitat. Crustaceans and mollusks were circumstantial components of cormorant diet, found in the gut of some cormorant fish prey, as shown from their δ15N isotope values. A mixing model, MixSIR, estimated that two fish species, Cetengraulisedentulous and Arius sp., were the main components of the diet. However, consumer stable isotope values fell outside the mixing polygon, suggesting that either another food source with a larger 15N value was present in the diet of the cormorant but we failed to detect it, or, that we used inaccurate discrimination factors in the model. This cormorant species is a trophic specialist. It appears that, at least during the studied period, this population fed only on fish from the lagoon or from the nearby sea.
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