Coprological Studies Research Articles

COMPARATIVE ASSESSMENT OF COMPLEX DRUGS BASED ON TOLTRAZURIL AND MOXIDECTIN AND TOLTRAZURIL AND EMODEPSIDE FOR NEMATODOSES AND CYSTOISOSPOROSIS OF DOGS

The article presents data on the effectiveness of the new domestic complex antiparasitic drug "Profilin coccid" (suspension for oral use) in comparison with the reference drug "Prokoks@ for dogs" when used for the treatment and prevention of endoparasitic infestations in dogs of various breeds, age, sex and body weight.
 According to the results of coprological studies, the animals of the experimental and control groups were found to have spontaneously infected dogs with endoparasites, the causative agents of digestive tract nematodes: Toxocara canis, with an intensity of invasion (II) of 100 to 190 eggs in one gram of feces (ЕGF) with an average intensity of invasion of 140.9 ± 4.07 ЕGF and Ancylostoma caninum with an intensity of invasion of 36 to 54 eggs in 1 g of feces with an average intensity of invasion of 44.9 ± 1.45 ЕGF, as well as coccidia of the alimentary canal of the species Cystoisospora canis with an intensity of invasion of 580 to 750 oocysts in 1 g of feces (OGF) with an average intensity of invasion of 677.5 ± 4.07 OGF. Clinically, nematodes of the alimentary canal in dogs and puppies were manifested by: loss of appetite, depression, pallor of the mucous membranes, and in some animals vomiting and mild diarrhea, and in cystoisosporosis - depression, motility, weight loss, during defecation - feces are liquid, sometimes with impurities of mucus.
 After the use of the drugs "Profilain coccid" and "Prokoks@ for dogs", coprological studies were carried out with the establishment of indices of parasitological damage and determination of the intensive effectiveness (IE) and extensive effectiveness (EE) of the drugs.
 On the basis of the obtained results, it was established that for a spontaneous toxocarosis infestation of dogs on the 7th day after deworming, the intensity of the drug "Profilin coccid" was 96.2 %, and the reference drug "Prokoks@ for dogs" was 96.9 %. On the 14th day after deworming the animals, the intensive and extensive effectiveness of both drugs was 100%.
 Helminthological studies established that for spontaneous hookworm infestation in dogs 7 and 14 days after the use of the researched drug and the reference drug in the recommended doses, the intensive and extensive effectiveness of veterinary drugs was 100 %.
 In case of spontaneous cystoisosporosis infestation in dogs, it was established that on 3 and 7 days after oral administration of the drugs to the animals, the intensity of the studied drug was 53.0 and 85.2 %, and the intensity of the reference drug was 56.0 and 87.3 %, respectively. On the 14th, 21st, 28th, and 35th day after the use of drugs in the form of a suspension, the intensive and extensive effectiveness for cystoisosporosis in dogs was 100 %.
 Field tests showed that the investigational drugs "Profilin coccid" and "Prokoks@ for dogs" are convenient to use and well tolerated by dogs and puppies, regardless of breed, age, sex and body weight, when they are used once orally in the medicinal form of a suspension in the dosage recommended by the manufacturer , and do not have a toxic effect on the body of animals.

Methods of coprological diagnostics of animal parasitoses

The purpose of the research is to review the classical methods of coprological diagnostics, taking into account modern recommendations and proposals. Materials and methods. Literature sources on the issues of coprological methods of diagnosing parasitosis have been studied. Results and discussion. Diagnosis during life is an important element of practical veterinary activity, the success of which largely depends on the correct choice and competent use of suitable laboratory methods. For the diagnosis of parasitic diseases, the study of feces is most often used. Coprological methods are highly informative due to the fact that they can detect not only parasites of the digestive tract, liver, pancreas, but also a number of helminths of the respiratory system and even some tissue parasites that excrete eggs or larvae with feces. Unlike existing indirect immunological and molecular genetic methods, classical research methods are based on the detection and identification of the pathogens themselves at different stages of development. At the same time, the researcher has the opportunity to study morphological features and conduct morphometric analysis. The widespread use of classical coprological methods is also associated with their availability, they are quite simple to perform and relatively inexpensive. A limitation of coprological methods is the inability to diagnose pathogens that are at a non-propagative stage. The effectiveness of diagnostics is influenced by the method of collecting samples, the terms and conditions of transportation, and the choice of an adequate method for examining samples. The article presents the rules for sampling and options for the use of fixatives, a classification of coprological methods is given, and the procedure for performing research is described. Special attention is paid to the choice of flotation solutions, the method of cultivation of strongylid larvae and the method of sporulation of coccidia oocysts. Attention is drawn to the importance of micrometric measurements of detected objects. To ensure the quality of the conducted coprological studies, it is necessary to control the stages of the studies, the technical condition of the equipment and train the personnel. The material presented in the article is intended for both students and practitioners.

Epizootic situation on horse helminthiases in the northern region of Kazakhstan.

The research relevance is predefined by the widespread distribution of parasitosis, including helminthiases, alongside constant depasture on the same areas of pastures, which annually causes massive re‑infection of horses. In this regard, the research aims to identify the age, seasonal, and territorial dynamics of infection of horses with helminthiases in the context of the districts of the northern region of Kazakhstan. The primary research method was an experimental analysis that allowing to consider the dynamics and causes of horse helminthiases, associated with the prepatent period, the resistance of the organism, and the system of keeping and feeding horses. The study presents the results of studies of horse helminthiases on the territory of the Northern region of Kazakhstan, covering 4 regions that currently exist in 61 agricultural formations. Faecal samples of 4,395 horses were examined by scatological methods and 126 samples - by incomplete helminthological autopsy. As a result of coprolarvoscopic and coprological studies, it was found that in horses, the dominant representatives among the invasive larvae of strongylates are small and large palisade worms. In scatological studies, strongylatosis of the gastrointestinal tract was recorded in 63.9% of cases, oxyurosis in 40.8%, and parascariasis in 25.9%. The study also presents the results of post‑mortem diagnostics, which was carried out during the slaughter or due to the death of the horse.

АНАЛІЗ ПОКАЗНИКІВ КРОВІ ТА КОПРОГРАМИ ПРИ ІНФЕКЦІЙНИХ ПАТОЛОГІЯХ ТРАВНОГО ТРАКТУ У СОБАК

Реакцію організму в разі будь-якого захворювання оцінюють не тільки за клінічними ознаками, але й за результатами досліджень біологічних субстратів організму. У процесі вивчення основних гематологічних показників собак, клінічно хворих на інфекційні патології травного тракту, виявлено помірну еритроцитопенію, стійку лейкоцитопенію, олігохромемію, гіпопротеїнемію, зниження кольорового показника крові, гематокритної величини та збільшення ШОЕ. Показники лейкограми свідчать про розвиток помірної нейтрофілії, чітко вираженої лімфоцитопенії, що призводить до виникнення імуносупресії, більш важкому перебігу хвороби. Копрограма собак із гострим перебігом інфекційних ентеропатій характеризувалась підвищеним вмістом білка, стеркобіліногену, білірубіну, неперетравленого крохмалю, мил і нейтрального жиру. Отже, зміни гематологічних показників і копрограми за інфекційних патологій травного тракту собак об’єктивно треба брати до уваги під час вибору терапевтичних засобів із метою ефективного їх лікування.

Infection of ducks kept in private subsidiary farms of Jalilabad district with mixed invasions

In the village of Badirri, Jalilabad district, coprological studies were conducted and 18.8% of ganguleteracidosis infections were detected among 3-month-old ducks, 31.3% among 6-month-olds, 43.8% among 9-month-olds. And in the village of Karayar, the examination revealed infection among 3-month-old ganguleterakis 36.0%, drepaidotenia 23.0%, amidostoma 27.2%, 6-month-old ganguleterakis 29.4%, drepanidotenia 17.1%, amidostoma 20.0%, 9-month-old ganguleterakis 30.0%, drepanidotenia 16.0%, amidostoma 25.0%, among adult ducks ganguleterakis 31.8%, drepanidotenia 12.0%, amidostoma 16.0%. Infection among ducks on the farm was 31.5% ganguleterakis, 16.6% drepanidoteenia and 22.0% amidostoma. In the farm, the highest infection rate of ducks in summer was 50.0%. According to the results of coprological surveys, the weakest infection in the farm was 6.7% in winter.

THE QUESTION OF EIMERIOSIS OF LAMBS IN SMALL FARMS

The article provides data on keeping lambs in small farms and homesteads in the Cherkasy region. Coprological studies have identified eimeria oocysts, in particular E. crandallis, E. intricate, E. arloingi, E. faurei, which are found in lambs and cause a lot of damage to their bodies. The presence of eimeria oocysts is associated with insufficient sanitary measures in cages and pens, which significantly affect the economic indicators of the profitability of small farms, which in turn limit the increase in the attractiveness of sheep farming. It has been established that eimeriosis in lambs often runs chronically, sometimes acutely. In lambs from the age of one month, characteristic clinical signs are observed: anemia of visible mucous membranes, diarrhea, depression, weakness and death. It has been noted that the intestinal epithelium of lambs is damaged during infestation. This worsens their assimilation of nutrients from food. It has been established that sick or sick lambs cause significant economic losses for such small farms, in particular, the profitability of wool, leather, and meat production decreases. It has been studied that at the beginning of the infestation, visible clinical signs in lambs depend on certain factors, in particular their age, weaning from the ewe, feeding, sanitary conditions in cages and pens, as well as various stressful situations. In older lambs, there is a tendency to infection with eіmeria. At the same time, eіmeriosis in them can be chronic or not clinically manifested, but Eimeria oocysts are excreted in their feces in a small amount, compared to one-month-old lambs during an acute course. In this regard, it is important, taking into account the time of year, to carry out diagnostic studies and medical and preventive measures for lambs of different age groups in small farms and homesteads.

Optimization of coprological studies in animals with the use of homeopathic Nux vomica 6CH

Background: Etiology of various gastrointestinal dysfunctions varies from infections and infestations to consequences of emotional stress and errors in animals’ nutrition. Taking this variability of etiology and pathogenesis into account, a lot depends not only on completeness of homeopathic history taking but also on the quality of laboratory studies. In our experience, coprology studies are especially complicated when persistent gastrointestinal disorders were preceded by massive use of allopathic remedies. This sometimes necessitates repeated examinations. That is why our interest in having reliable results and early detection of latent indigestion made us look for non-invasive methods which could be easy to use in animals but at the same time could reveal latent gastrointestinal pathology in animals. Aims: The study was aimed to determine the effect of a homeopathic medication Nux Vomica 6CH on detection of latent gastrointestinal pathology in small domestic animals. Method: Coprology studies were performed simultaneously with the use of sedimentation method and Fulleborn method (modification of Scherbovich). Infestations were diagnosed and characteristics of patient digestion were recorded. Feces test detected: 1. Presence of endoparasites (helminths, protozoa and yeast-like structures); 2. State of intestinal microflora (presence of different species and their balance with each other); 3. Degree of food absorbency (presence or lack of undigested protein, carbohydrates and fat); 4. Presence of inedible inclusions; 5. Presence of cells (lymphocytes, erythrocytes, intestinal epithelium, tumor fragments). If animal feces tests were non-informative, then all medications (except vitally important) were discontinued and Nux Vomica 6CH twice a day twenty minutes prior to food intake was prescribed for 6 days (4 globules dissolved in 10 ml of water). Results: 4500 coprology tests were performed during 3 years in cats and dogs. About 10% of the total number of examined animals (449 patients) produced very little information about the state of gastrointestinal systems at the first examination. When these cases were analyzed, the following reasons were found: long-term use of strong drugs, significant weakening of body immune system. As a result diagnostic tests were of very little value. These animals were treated by Nux Vomica 6CH for 6 days and then repeated feces test was performed. This produced a more informative picture of the state of gastrointestinal system and enabled to determine etiology and pathogenesis of the animal condition and prescribe necessary treatment. Conclusion. In such a way, Nux Vomica 6CH homeopathic remedy promoted effective optimization of coprology studies in animals. The suggested scheme of use for Nux Vomica 6CH significantly improves feces test quality. This in turn reduces time needed to choose strategy and tactics of treatment of small domestic animals with different chronic diseases.

Necropsies disclose a low helminth parasite diversity in periurban howler monkeys.

Primate-parasite interactions are often investigated via coprological studies given ethical and conservation restrictions of collecting primate hosts. Yet, these studies are inadequate to recover adult helminths for taxonomic identification and to accurately assess their prevalence, intensity, abundance, and site of infection. Fresh carcasses found in anthropogenic landscapes come as informative and reliable alternatives. In this study, we identified the helminths of brown howler monkeys (Alouatta guariba clamitans) and their sites of infection, and measured their prevalence, intensity, and abundance of infection. We necropsied 18 adult males, 11 adult females, and 7 juvenile males that died in conflicts with the anthropogenic environment (domestic dog attacks, n = 11; electrocutions and road-kills, n = 10 each; unknown, n = 5) in periurban landscapes of southern Brazil between 2013 and 2019. We found three nematodes (Trypanoxyuris minutus, Dipetalonema gracile, and Parabronema bonnei) and one cestode (Bertiella cf. studeri), a diversity estimated to account for a sampling completeness of 99%. Prevalence ranged from 3% for P. bonnei to 100% for T. minutus. Mean abundance ranged from 2 (D. gracile and B. cf. studeri) to 55,116 (T. minutus) and mean intensity of infection ranged from 4 (B. cf. studeri) to 55,116 (T. minutus). Trypanoxyuris minutus sex ratio was strongly male-biased. The intensity of infection with T. minutus was higher in juvenile males and adult females than in adult males. The low parasite diversity and the helminths' mode of transmission are compatible with howlers' arboreality and folivorous-frugivorous diet. The howlers were not infected with soil-transmitted helminth parasites of humans and domestic animals on the ground and probably did not eat invertebrates to complement the diet. Given the lack of evidence of howler health problems, we suggest that the causes of death of the necropsied howlers are the major threats to the long-term conservation of the species at the study periurban landscapes.

Prevalence of gastrointestinal helminth parasites in domestic ruminants from Srikakulam district, Andhra Pradesh, India.

Coprological studies on the prevalence of gastrointestinal helminth (GI) parasites in domestic ruminants from Srikakulam district of Andhra Pradesh, India, were carried out for a period of one year from January 2019 to December 2019. Fecal examination was done using direct smear, sedimentation and floatation methods. Altogether 3527 fecal samples were examined including 595 from cows, 485 from buffaloes, 1342 from sheep and 1105 from goat. Out of 3527 fecal samples examined, 1084 were found to be positive with three groups of gastrointestinal parasites (GI) showing an overall prevalence of 30.7%. Maximum infection was noted with nematodes (39.1%) and trematodes (37.3%), least infection was noted with cestodes (3.5%) and mixed infections were found to be moderate (20.01%). Host wise prevalence of parasitic infection has shown highest in the case of cattle recording 43.03%, followed by 40.8% in buffaloes, 29.4% in sheep, and 21.4% in goats. Total six varieties of parasites were recorded, the predominant being Haemonchus (Strongyle group) (29.25%), followed by Paramphistomum (25.5%), Fasciola (11.9%), Strongyloides (7.6%), Moniezia (3.5%) and Trichuris (2.2%). Season wise data on prevalence showed, high rate of infection in summer season (32.0%) when compared to winter (30.2%) and rainy (29.9%) seasons. Further studies are needed to design a rational for sustainable management of GI parasite infections in domestic animals of local regions.

Helminthiases of wild and domestic pigs of the Trans-Baikal Territory and measures to control them

The paper presents the results of coprological studies and complete helminthological autopsies (2015–2019) to identify sources of infection in pigs, parasites of the Nematoda class, Ascaris suum, Oesophagostomum dentatum, Trichocephalus suis, Strongyloides ransomi . A complex drug based on a chemotherapeutic agent (fenbendazole) and medicinal herbs (common wormwood and St. John's wort) was developed, which has a pronounced anthelmintic effect against nematodes and a symptomatic effect on concomitant diseases of the respiratory and gastrointestinal tract. At the first stage, the study of the effectiveness of the developed preparation was carried out on domestic pigs. The use of the developed agent on two-month-old piglets allows to free the body from worms completely. In four-month-old piglets, it has a 100% detrimental effect on strongyloid, esophagostoma and trichocephalus, 80% – on ascarids. It was noted that with ascariasis, the intensity of the developed agent is 16% higher than that of Ivermec. Both drugs had the same 100% internal efficacy against strongyloidosis, esophagostomosis and trichocephalosis in terms of the percentage of isolated helminths, eggs or larvae in relation to their number before the application of the anthelmintic. The external efficacy of both drugs in terms of the percentage of animals completely freed from helminths after treatment was 80% against ascariasis, and 100% against strongyloidosis, esophagostomosis and trichocephalosis. The therapeutic efficacy of the developed medical and prophylactic agent against helminthiasis of wild pigs was studied on the territory of the hunting farm of the Trans-Baikal Territory. A decrease in the infection of wild pigs due to the use of the drug was recorded: internal efficacy against ascariasis was 50–66%, against strongyloidosis, esophagostomosis and trichocephalosis 100%. External efficacy was 80%, indicating a 20% reduction of disease rate in wild pigs. The agent for the prevention and treatment of pig helminthiases allows to reduce the morbidity of animals and ensure the production of products that are safe in veterinary and sanitary terms.

Efficacy of anthelmintic Eprimek against gastrointestinal nematodes in sheep

The purpose of the research is studying the efficacy of Eprimek (Eprinomectin) against gastrointestinal nematodes in sheep.Materials and methods. A commercial experiment to study the antiparasitic efficacy of Eprimek was carried out in June 2020 on 300 lambs of the North Caucasian breed in a private flock of Filimonovskaya Village, Izobilnensky District, the Stavropol Territory, which were divided into two groups. The experimental group of lambs (290 animals) was injected Eprimek subcutaneously at the earset at a dose of 1 ml/50 kg of live weight (10 mg of Eprinomectin in 1 ml), and 10 lambs were not treated; they were used as control. We collected feces from the lambs of the experimental and control groups before administration of the drugs and after 15 and 30 days. Fecal samples were examined by the flotation technique with a saturated solution of ammonium nitrate with counting nematode eggs in 1 g of feces. The results were processed statistically.Results and discussion. Eprimek showed a decrease in the number of excreted helminth eggs from 225.1±28.2 to 4.1±2.3 in production environment at a dose of 1 ml/50 kg of live weight, according to coprological studies on the 15th day after treatment in the experimental group of lambs. The efficacy was 98.2%, and 70% of the animals were free from the infection. The intensity of infection of the control lambs by gastrointestinal nematodes was 131–151 eggs per 1 g of feces at 100% prevalence.

Identificación de nematodos gastrointestinales en búfalos faenados en un frigorífico de Corrientes, Argentina

<p>El objetivo del trabajo fue identificar y cuantificar los ejemplares adultos de nematodos gastrointestinales en los búfalos faenados en el frigorífico de Virasoro (Corrientes) mediante la necropsia parasitaria del tubo digestivo y, por intermedio de estudios coprológicos, correlacionarlos con el recuento de huevos por gramo de materia fecal y la proporción de géneros parasitarios de larvas de tercer estadio de los coprocultivos. Se realizaron en total 4 necropsias parasitarias que correspondieron a búfalos machos jóvenes, de los cuales el 50% presentó ejemplares adultos de Trichostrongylus sp y Haemonchus sp ubicados solamente en el abomaso. En los estudios coprológicos, el 75% de las muestras presentaron recuentos de huevos por gramo de materia fecal, de los cuales únicamente en dos casos se pudieron identificar larvas de tercer estadio de Haemonchus sp en los coprocultivos.</p>

Epidemiological distribution of Echinococcus granulosus s.l. infection in human and domestic animal hosts in European Mediterranean and Balkan countries: A systematic review.

Cystic echinococcosis (CE) is a neglected zoonosis caused by infection with the cestode Echinococcus granulosus sensu lato. We carried out a systematic literature review on E. granulosus s.l. human and animal (cattle, sheep, dog) infection in European Mediterranean and Balkan countries in 2000-2019, to provide a picture of its recent epidemiology in this endemic area. MEDLINE, EMBASE, Scopus, Google Scholar and Open Grey databases were searched. Included cases were: i) for humans, data from hospital records and imaging studies; ii) for dogs, data from necropsy and coprological studies; iii) for ruminants, cases based on slaughter inspection. The NUTS (Nomenclature of Territorial Units for Statistics) classification was used to categorize extracted data in epidemiological units, defined as data referred to one NUTS2 (basic region) in one year time. Data were then aggregated to NUTS1 level (major regions), calculating the average incidence value of included epidemiological units. For prevalence studies covering different epidemiological units, the pooled prevalence was estimated. Data were extracted from 79 publications, 25 on human infection (covering 437 epidemiological units), and 54 on animal infection (52 epidemiological units for cattle, 35 for sheep and 25 for dogs). At NUTS1 level, average annual incidence rates of human CE ranged from 0.10-7.74/100,000; pooled prevalence values ranged from 0.003-64.09% in cattle, 0.004-68.73% in sheep, and 0-31.86% in dogs. Southern and insular Italy, central Spain, Romania and Bulgaria reported the highest values. Bovine data showed a more similar pattern to human data compared to sheep and dogs. Limitation of evidence included the paucity of human prevalence studies, data heterogeneity, and the patchy geographical coverage, with lack of data especially for the Balkans. Our results confirm Italy, Spain, and Eastern Europe being the most affected areas, but data are extremely heterogeneous, geographical coverage very patchy, and human prevalence studies extremely scant. Results also highlight the notorious problem of underreporting of E. granulosus s.l. infection in both humans and animals.

Mixed course of Fasciolosis and Paramphistomatosis in sheep in Uzbekistan

The purpose of the research to study the mixed course of sheep infection, caused by pathogens of fasciolosis and paraphistomatosis, in the conditions of Samarkand and Tashkent regions. Materials and methods. The research material was fallen and forcibly killed sheep from two regions of Uzbekistan, simultaneously infected by Fasciola gigantica and Paramphistomum spp. (Gastrothylax crumenifer, Calicophoron calicophorum). The animals' liver was subjected to complete helminthological dissection, followed by the presence of young immature in the parenchyma, and in the bile ducts – sexually mature fasciola. To detect pathogens of paramphistomatosis, we examined the scar and mesh, and, if necessary, the mucous membranes of the abomasum and the initial part of the small intestine. To kill the collected F. gigantica and complete bleeding with a Paramphistomum spp., they were kept in water for one and three to four days, respectively. After they took their natural form, their type, age and number were determined, and then fixed in Barbagallo fluid. If necessary, coprological studies of sheep and malacological studies of biotopes of freshwater mollusks, intermediate hosts of the studied trematodes, were carried out. Results and discussion. In recent years, cases of a mixed course in sheep fasciolosis (F. gigantica) and paramphistomatosis in farms of Uzbekistan have become more frequent. When opening the liver of the fallen sheep, up to 278 specimens were found immature F. gigantica and up to 3994 sp. G. crumenifer. In some farms, the death of individual owners' sheep from calicophorosis caused by C. calicophorum was observed with an intensity of infection of 592 sp. of C. calicophorum.

CASES OF ASCARIASIS IN THE KRASNODAR REGION IN THE PERIOD FROM 2015 TO 2019

To identify the epidemiological situation in the Krasnodar region, the following activities were carried out: a) analysis of statistical data kindly provided to us by the Office of the Federal state statistics Service for the Krasnodar region in the last five years (2015–2019); b) analysis of the results of coprological studies of faecal smears; as well as the results of blood serum tests for serological diagnostics of children and adults, conducted in the laboratories of the GBUZ regional hospital No. 2 and KBUZ Research Institute – Regional clinical hospital No. 1, as well as in the laboratory of the emergency care hospital of Krasnodar city. Based on our research, we identified two categories of the population, of different age groups, most susceptible to the disease ascariasis. The first group is children 7–16 years old, students of Junior and senior classes of city schools and the second group is adults – from 18 years and older, students of colleges, technical schools, and working people. In the first group, the peak of infestation of children with ascariasis occurred in 2018, it reached 23.1% per 100,000 people surveyed. In 2019, it was lower than 22.8%, but still quite large. In the second group, the rise of the disease occurred in 2016, it amounted to 17.5%, in subsequent years, the decline of ascariasis is noticeable.

СЕЗОННО-ВОЗРАСТНАЯ ДИНАМИКА ЭЙМЕРИОЗА И КРИПТОСПОРИДИОЗА КРУПНОГО РОГАТОГО СКОТА

Currently, the topic of determination of the seasonal and age-related dynamics of eimeria and cryptosporidia, especially with their associative course in cattle on farms of the Udmurt Republic, remains relevant. The research material were represented by fecal samples (1629 samples) from animals of various age groups of the central zone of Udmurtia, spontaneously infected with eime-ria and cryptosporidia. Coprological studies were performed monthly by generally accepted flotation methods, and fingerprint smears were made, followed by Ziehl-Nielsen staining. Our studies on the seasonal dynamics of protozoa showed that peaks of infection in animals in the central zone of the Udmurt Republic were noted in the autumn-winter periode. The age dynamics in the regions differed significantly depending on the type of pathogen and the age of the animals. Thus, Zavyalovsky is a stationary dysfunctional area for eimeriosis and cryptosporidiosis, where the per-centage of eimeriosis infection is 96.3%. At the same time, the maximum number of ocists were observed in young animals in age of 8-12 months. Cryptosporidiosis infestation was recorded from 3 days of age to 2 months, and the percentage of infection in this age group reached 89,66%.The results of our studies revealed a fairly widespread association of these protozooses. So, it was found that in Zavyalovsky district, the maximum intensi-ty of mixtinvasion is recorded in animals aged 1-2 months and reaches up to 69,17%. In the Uvinsky district, the greatest degree of infection with the association of eimeria and cryptosporidia is record-ed almost 2 times less often (in 37,31% of cas-es).The results of seasonal dynamics must be taken into account for drawing up treatment and preventive measures for various age groups of animals in the central zone of the Udmurt Republic.

Endoparasitic fauna of two South American foxes in Chile: Lycalopex culpaeus and Lycalopex griseus.

Native foxes face serious threats related to anthropic activities and the uncontrolled pets. However, the transmission of several pathogens such as parasites by domestic dogs is an important conservation issue with limited attention in Chile. The lack of a proper identification of the helminths of wild canids in the country, based mostly in coprological studies, limited our knowledge in potential interspecific transmission. Thus, the aim of the present survey was to identify the parasitic fauna of two native canids, the Andean fox (Lycalopex culpaeus) (n=49) and Chilla (Lycalopex griseus) (n=17), from several localities in Chile through a complete parasitological survey. Sixteen different species were identified, 9 of which are new host records for Andean fox and two for Chilla fox in South America, and three are recorded for first time in Chile. Also, five of them are of zoonotic concern and six are known to use domestic dogs as their principal hosts. These findings have implications for public health and the conservation of canids whose interspecific transmission of parasites with domestic dogs is confirmed through these findings. The need for an exhaustive surveillance of wild carnivores is emphasized considering these hosts as important sources of micro- and macroparasites.

МОНИЗЕН® ФОРТЕ ПРИ ПАРАЗИТАРНЫХ БОЛЕЗНЯХ ОВЕЦ

The Monizen Forte test for parasitic sheep diseases was tested under production condi-tions on 460 lambs of the North Caucasian breed, with a live weight of 25-30 kg, with high infection with nematodes, cestodes and larvae of the nasopharyngeal gadfly (Oestrus ovis), which were given orally in a mixture with half the normal rate of concentrated feed, the test drug in a dose of 1 ml per 20 kg of the animal weight. Experienced (20 ani-mals) and control (10 animals) groups of lambs with an average live weight of 28 kg were selected from these lambs according to the principle of analogues. To the lambs of the control group, the drug was not given. 10 days after administration of the drug by clini-cal and coprological studies, it was found that the lambs of the experimental group, the intensity of nematode invasion decreased and the drug’s intensity (IE) was 96.7%, and the extensivity (EE) was 95%. The lambs of the experimental group before the introduction of the drug were 40% invaded with moniesia and 50% dictiocauli, and after using Monizen Forte they were released - the ex-tensibility (EE) was 100%. Lambs of the control (untreated) group, the intensity and extent of infection with nematodes and ces-todes remained at the same level as at the beginning of the experiment. All 10 lambs (50%) of the experimental group, the clinical signs of estrosis (sneezing, snorting, serous-mucous discharge from the nose) disap- peared, and the lambs of the control group have these clinical signs persisted.

Effectiveness of the new Iverlong prolonged-release drug for gastrointestinal nematodoses in sheep

The article presents the results of experiment on testing a prolonged form of the Iverlong drug against gastrointestinal nematodes in sheep. The work was carried out on 30 lambs. The lambs of the first group were subcutaneously injected with the Iverlong preparation at a dose of 1 ml/50 kg of live weight (in 1 ml of 50 mg of Ivermectin plus PLGA prolongator), the lambs of the second group were treated with the same preparation, but at a dose of 0.5 ml/50 kg of live weight. The lambs of the third group served as a control, which were subcutaneously injected with the well-known Ivomek drug in the recommended dose (1 ml/50 kg of live weight). The effectiveness from the prolonged form of Iverlong in 2 doses against nematodes of the gastrointestinal tract was taken into account by means of coprological studies before drug administration and every 15 days for three months. According to the results of coprological studies, it was found that the Iverlong drug in a dose of 1ml/50 kg of the animal’s live weight showed a high prolonged anthelmintic effect and for 60 days protected animals from infection by nematodes of the gastrointestinal tract.

Integrated treatment of cattle in fasсiolosis

One of the reasons for the unprofi tability of dairy cattle breeding is fasciolose invasion. This disease can cause signifi cant economic damage in the form of a decrease in the milk production of cows, a decrease in the growth of young animals and a negative impact on the reproduction and renewal of the herd. The domestic veterinary market is saturated with a large number of various anti-trematode drugs of both foreign and domestic production, however, the price/eff ectiveness of anthelmintics remains a key aspect for animal owners. However, against parasitic drugs, they only destroy the parasite in the body of sick animals, without pouring in on the processes of recovery of the body after invasion. Longterm use of the same drugs, violation of the instructions for use of the latter leads to an increase in the number of reports of scientists from around the world about the increase in the number of cases of development of fasciol resistance in anthelmintics. That is why the search and testing of new therapeutic combinations that ensure the release of cattle from trematodes and helped restore disturbed physiological functions remain relevant today. The study presents the results of testing the use of a combination of rafenzene emulsion and infusion of St. John's wort on cattle fascioliasis in the conditions of the dairy industry of the Kyiv region. The purpose of the study was to study the therapeutic effi cacy of a combination of aetiotropic and pathogenetic therapies of calves for fasciulosis - rafenzol emulsion and infusion of herbs with St. John's wort. The young cattle of the black-bred breed, aged 12-18 months, were examined and their faeces sampled. The calves were monitored for 30 days during the experiment. They paid attention to the general condition. Heart rate, body temperature and respiratory rate were measured. Studies of individual organs and systems were performed according to conventional methods. Coprological studies were performed on the 10th and 30th day of observation. Used the method of sequential fl ushing faces. Calves, one of which was a control, was formed to study the eff ectiveness of antitrematodes measures. In each of them there were 5 animals, aff ected by fascioles, selected on the principle of analogues. The calves of the fi rst group were administered rafenzol emulsion at a dose of 0.75 ml of the preparation per 10 kg of body weight inside once, the animals of group 2 used the drug similar to the fi rst group. Previously they were fed with infusion of herbs St. John's wort. The control group was not treated.The results of the study indicate that the young is large aff ected with fascioles and the average invasion intensity is 48% and the intensity is 17 copies of eggs, respectively. Complex use of rafenzol emulsion with infusion of herbs with St. John's wort, according to the scheme, after 80 days revealed 80% of the extent and intensity. After 30 days, the indicator improved to 100%. Extensibility and intensifi cation of one rafenzol emulsion, after 10 days was 40%, for 30 days the effi ciency increased to 80%. St. John's wort improves the regeneration of tissue-damaged fasciolosis. It contributes to the absorption of feed and restore productivity. Therefore, treatment and prevention of fasciulosis in the farm is best done comprehensively. Key words: rafenzene emulsion, st. John's wort herb infusion, fasciolosis, disease intensity and intensity, effi cacy and drug intensity.