A study was made of the relative thiamine and riboflavin content of whole wheat, nonenriched and enriched flour of common origin and of breads made therefrom. Four hundred and fifty young rats constituting 38 experimental groups were used in the study. All animals were subjected to a 2-week adaptation period before being placed on experiment. The feeding period was of 8 weeks duration unless death of experimental subject intervened. The results, as a whole, show that flour, enriched in accordance with the present formula, is definitely superior to nonenriched flour with respect to thiamine and riboflavin, and is somewhat superior to whole wheat with respect to these vitamins. While the amount of thiamine contributed by ground whole wheat and by enriched flour, when the flours composed 30% of the diet, is only slightly less than that required for optimal growth in young rats, the amount of riboflavin contributed is definitely insufficient. However, the enriched flour was found to contain more riboflavin than the original wheat. Breads made from ground wheat and from enriched flour show good retention of thiamine and riboflavin as indicated by growth tests and by vitamin assays. Dark bread and milk bread, as prepared and used in these studies, were found to be definitely inferior to breads made from ground whole wheat or from enriched flour as sources of thiamine, and somewhat less inferior as sources of riboflavin.
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