This research focused on measuring the concentration of particulate matter (PM2.5) aerosols and radionuclides (226Ra, 232Th, and 40K) in road dust collected using glass fiber filters from busy roads and populated areas in Islamabad and Rawalpindi, Pakistan. The measured concentrations ranged between 551.0 and 2025.7 µgm−3 for PM2.5 aerosols, 32.4 and 54.7 mBqm−3 for 226Ra, 15.8 and 26.8 mBqm−3 for 232Th, and 239.0 and 316.4 mBqm−3 for 40K. The study found that PM2.5 levels surpassed the WHO’s recommended limit of 25 µgm−3. Additionally, concentrations of 226Ra and 232Th were considerably higher than the global average values of 1 and 0.5 µBqm−3, respectively, in dust. The total inhalation effective dose in all samples exceeded the ICRP’s acceptable limit of 2 mSvy-1, except in the infant and 1-year-old age groups (considering F, M, and S-type modes). Notably, 232Th was identified as the primary contributor, accounting for 36.5 % to 99.4 % of the total inhalation effective dose.