Content Of Green Pigments Research Articles

Вміст пластидних пігментів у дерев сосни звичайної уражених сосновим вертуном в умовах Західного Полісся

The biosynthesis of plastid pigments in Pinus sylvestrys L.was investigated in pine trees affected by Melampsora pinitorqua in the conditions of the Western Polissya. The total content of chlorophylls and carotenoids in the needles of trees with a mild and moderate degree of damage by Melampsora pinitorqua is 13,3…30,0% lower than in the control. Trees severely affected by Melampsora pinitorqua synthesize 21.8...38.4% less green and yellow pigments. A decrease in the content of green pigments in experimental pine trees is caused by chlorophyll a. Іt influences on absorption direct sunlight for the process of photosynthesis. The ratio of chlorophylls a/b is close to the control in experimental trees with a mild and moderate degree of disease damage and decreases by 23.3...32.9% in trees with a severe degree of disease damage. Іn all affected trees, the ratio between the amount of green and yellow pigments is close to the control.

Investigation of the response of sweet cherries to root mycorrhisation with biologics for sustainable horticulture development

Sustainable horticulture development is a priority area considering climate change, especially in the context of reduced water supply. The search for ways to regulate the resistance of fruit trees to stressors is an urgent issue for the world community. The use of beneficial microorganisms to inoculate plant roots helps reduce the use of synthetic substances and is an alternative for sustainable horticulture. The purpose of the study is to identify the specific features of the effect of root inoculation by mycorrhizal fungi on the reaction of sweet cherries to develop strategies for managing the production of fruit products. The study was conducted during 2018- 2020 in the sweet sweet cherry orchard of the southern steppe subzone of Ukraine. Such research methods as field, laboratory, biochemical, physiological, and statistical were used. The regularities of the influence of mycorrhizal fungi on the total moisture content and water-holding capacity of sweet cherry leaves were examined. In the first year of studies on endomycorrhizal root inoculation, these indicators were higher compared to the control group. The positive effect of endo-ectomycorrhiza on the waterholding capacity of sweet cherry leaves was elucidated. The total leaf surface and the content of green pigments in the leaves were determined as one of the main indicators of the photosynthetic potential of a fruit crop. The effect of inoculation of sweet cherry roots by mycorrhizal fungi on the ratio of chlorophylls (a/b) in leaves was established. The parameters of the content of total nitrogen, phosphorus, and potassium in sweet cherry leaves were determined. The use of mycorrhizal fungi increases the adaptive properties of sweet cherry trees to stressful factors, namely, arid conditions in the south of Ukraine. The research results can be used by fruit producers to improve climate-optimised technologies, which will substantially reduce risks and possible economic losses, and preserve biodiversity

Protective effect of zinc complex with hypoxanthine-9-riboside on wheat seedlings grown from gamma-irradiated seeds

Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl2 with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a 60Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.

Components of photosynthetic activity of cherry trees in the Right-Bank Forest-Steppe of Ukraine

The article presents the results of research on the photosynthetic activity of cherry trees. The aim of the research was to study the morphological, growth and biochemical characteristics of the leaf cover of cherry trees depending on the time of fruit ripening. Observations, analysis and laboratory studies reveal morphological and biochemical features of annual tree growth, total leaf surface and content of green pigments in the leaves which is one of the main indicators of photosynthetic potential of fruit trees. It was established that Dar Mlieva was characterized by the largest total length of annual increments among early ripening varieties, Melitopolʹsʹka krapchasta variety – among medium ripening varieties, and Drogana zhovta variety among late ripening varieties. Aborehenka variety had the largest average length of shoots, and Mirage variety had the smallest length. The number of shoots on the tree and the activity of annual new ramules make the shoot-forming ability. Cherry varieties are divided into groups according to the obtained data: with a high degree of shoot formation – Dar Mlieva, Zoryana, Mirage, Melitopolʹsʹka krapchasta and Drogana zhovta; middle degree – Mliivsky zhovta, Aboriginal, Meotida, Amazon and low degree – varieties Alyonushka, Biryuza, Donetsky uholʹok. Low leaf area of cherry plantations negatively affected the activity of the processes of photosynthesis and productivity of plantations aged 6–8 years and made 14–35 % of the optimal. The content of chlorophyll in the leaves depended largely on the characteristics of the pomological variety, while for the maturity of the fruit, the dependence was not detected. The middle maturity cherry varieties of Mirage and Meotida differed favorably in the content in the leaf of pigments of photosynthesis of carotenoids, chlorophyll a, b, as well as the sum of a + bchlorophylls . The content of these indicators exceeded the indicators of other studied varieties by 25 % on average. Key words: sweet cherry, photosynthesis, total leaf surface, length of annual increments, chlorophyll content.

EFFECT OF EXCESS IRON (II) IONS ON PHOTOSYNTHESIS PROCESSES IN BARLEY PLANTS

It is known that all heavy metals (TM), including iron, affect the development of crops. At the same time TM affect both morphometric parameters of plants and physiological processes taking place in them. However, these processes and their changes as a result of adverse factors may differ not only in different plant species, but also in different varieties. This paper presents a study of changes in the content of chlorophylls a and b in leaves of different varieties of barley plants when excessive doses of iron (II) ions are added to the growing medium. As a result of the study, it was revealed that Fe2+ had a different effect on the content of green pigments and their ratio in the studied varieties of barley. At the same time, some varieties turned out to be more stable than others. In general, the content of chlorophyll a in the control version ranged from 17.45 (Belgorod 100) to 22.16 (29-11) mg/g; chlorophyll b - from 9.30 (Bionic) to 12.95 (29-11) mg/g. Photosynthesis processes are associated with the formation of proteins, fats and carbohydrates in plants. Therefore, this study will serve as a further basis for studying the relationship between disruptions in the processes of photosynthesis in crops and the formation of organic substances in them.

Impact of NaCl on physiological and biochemical characteristics of maize (Zea mays L.) plants

Aim. The effect of 50 and 100 mmol NaCl concentrations on seed germination, the content of green pigments in seedlings, and on the activity of photosystem II in seedlings of maize varieties Zagatala 420, Zagatala 514, Zagatala 68 and Gurur and hybrid Gurur x Zagatala 68 was studied. Methods. Plant seeds were germinated under laboratory conditions in Petri dishes and pots with soil using 50 and 100 mmol of sodium chloride solutions. Germination, seed germination energy had been determined. In two-week old seedlings determined the number of photosynthetic pigments and the activity of photosystem II. Results. High concentrations (100 mmol) of NaCl reduced seed germination, pigments number, and photosystem II activity. At a salt concentration of 100 mmol, an increase in the carbohydrate content was noted. Conclusions. Varieties Gurur and Zagatala 68 and hybrid Gurur x Zagatala 68 were more tolerant compared to varieties Zagatala 420, Zagatala 514.
 Keywords: maize, NaCl, photosynthetic pigments, photosystem II.

Influence of NaCI on Seed Germination, Content of Photosynthetic Pigments, Sugars, and Activity of Photosystem II in Maize (Zea mays L.) Leaves

The effect of 50 and 100 mmol NaCl concentrations on seed germination, the content of green pigments in seedlings, and on the activity of photosystem 2 in seedlings of maize varieties Zagatala 420, Zagatala 514, Zagatala 68 and Gurur and hybrid Gurur x Zagatala 68 was studied. Plant seeds were germinated under laboratory conditions in Petri dishes and pots with soil using 50 and 100 mmol of sodium chloride solutions. In two-week old seedlings determined the number of photosynthetic pigments and the activity of photosystem II. High concentrations (100 mmol) of NaCl reduced seed germination, pigments number, and photosystem II activity. At a salt concentration of 100 mmol, an increase in the carbohydrate content was noted. Varieties Gurur and Zagatala 68 and their hybrid “Gurur x Zagatala 68” were more tolerant compared to varieties Zagatala 420, and Zagatala 514.

Аналіз життєвості вуличних насаджень після омолоджувальної обрізки (на прикладі м. Дніпро)

The influence of pruning of the crown on the state of five tree species has been studied (Robinia pseudoacacia L., Ulmus pumila L., Gleditsia triacanthos L., Acer pseudoplatanus L. and Populus bolleana Lauch.). The trees were pruning at a height of 8 meters, leaving only the trunk and lower branches. Control plants were not pruned. All plants are growing on the boulevard near the highway with high traffic. It was found that among the rejuvenated trees there are more both healthy plants and severely damaged ones. Dead plants appeared among species such as G. triacanthos, A. pseudoplatanus, and R. pseudoacacia also. The best vital state was found in P. bolleana and U. pumila. Plants after crowning have more water content in leaves. U. pumila and G. triacanthos have the greatest difference in water content between the two variants. The intensity of transpiration in pruning plants P. bolleana and G. triacanthos is 2 times higher, and in U. pumila – by 20 %. In A. pseudoplatanus and R. pseudoacacia, there is no difference in this indicator between the variants. Pruned plants have a lower water-holding capacity by an average of 30 % compared to control (except for P. bolleana, in which the difference between the variants is insignificant). The content of green pigments is higher than in the control samples in the leaves of the rejuvenated plants. The exception is R. pseudoacacia, which has a reverse reaction. Thus, the intensification of the processes of water exchange and the pigment system does not occur in all species to the same extent. It is most pronounced in U. pumila and G. triacanthos. The activity of photosynthesis and water exchange in both variants differs insignificantly at P. bolleana. R. pseudoacacia have deterioration in all parameters. This probably explains the poor condition of pruned trees of this species.

Influence of multicomponent contamination on the content of photosynthetic pigments in the leaves of woody plants commonly planted for greening of cities

Woody plants, as an important element of optimization of the urban environment, respond negatively to the ever-increasing technogenic pressure in cities. Therefore, it is necessary to assess their vital status, the most sensitive indicator of which being the content of plastid pigments. In this article we analyze the effects of multicomponent contamination on quantitative fluctuation of the level of photosynthetic pigments in the leaves of woody plants and identify sensitive species for the purpose of phytoindication in order to assess the state the environment is currently in. For the purposes of our research we chose the most widespread tree species in the city of Dnipro (Steppe zone of Ukraine), growing in the area of intensive industrial pollution and high levels of emissions by vehicles. We determined the content of photosynthetic pigments (chlorophylls a and b, a + b, carotenoids) in the leaves of woody plants during different months of the growing season (June, July, August) and afterwards this data was used to compare the given parameters against the values taken from trees in the control area. The sum of chlorophyll levels a + b for the majority of plants was decreasing compared to the control. A more significant decrease in the content given by the sum of the a and b chlorophyll levels is observed in the assimilation organs of Aesculus hippocastanum L., Pinus pallasiana (D. Don) and Picea abies (L.) H. The most significant changes in the content of pigments in the leaves of plants under study were detected at the end of the growing season (in August). The content of green pigments in the leaves of Robinia pseudoacacia L., Platanus orientalis L. remains virtually unchanged. Under the influence of multicomponent contamination, the amount of pigments in the case of chlorophyll a in the majority of woody plant species decreases more rapidly compared to the control than in the case of chlorophyll b. The most noticeable drop in the content of chlorophyll a occurs in the leaves of Betula pendula Roth, Ae. hippocastanum, P. pallasiana, Acer platanoides L. and Tilia cordata Mill. The needles of P. abies and Picea pungens Engelm. 'Glauca’ on the contrary suffer from a significant reduction in the concentration of chlorophyll b. The species most resistant to multicomponent contamination in terms of changes in the content of chlorophyll in the leaf blade are R. pseudoacacia, P. orientalis. The concentration of carotenoids in the leaves of plants such as B. pendula, Ae. hippocastanum, A. platanoides, T. cordata, P. abies is lower than that of species growing in the relatively clean zone. The concentration of pigments in Populus alba L., P. pungens 'Glauca’ was higher during all of the months elapsed since the beginning of our study, whereas Ulmus laevis Pall., P. orientalis and R. pseudoacacia – had their concentrations reach an all time high in the second half of the growing season. Therefore, the peculiarities of quantitative fluctuation of photosynthetic pigments in plants, which are characterized by the variable resistance ability against the effects of technogenic multicomponent contamination, have been identified.

Content of Plastid Pigments in the Needles of Pinus Pallasiana D. Don in Different Forest Growth Conditions of Anti-Erosion Planting

Abstract The influence of different forest growth conditions on the content of plastid pigments in Pinus pallasiana D. Don needles in the anti-erosion planting of the steppe zone of Ukraine was investigated. The reduction in the total chlorophyll a and b concentration in the pine needles in the area with insufficient water supply (xerophilous and mesoxerophilous hygrotopes) was found, compared with more favourable conditions for water supply (mesophilous hygrotope). The reduction in the content of green pigments in arid conditions is due to chlorophyll a. Chlorophyll b showed high resistance to moisture deficiency. There is an increase in the concentration of carotenoids in pine needles in forest growth conditions with insufficient water supply (xerophilous and mesoxerophilous), compared with more favourable conditions (mesophilous), which can be explained by the adaptive role of these pigments in the processes of stabilising the functioning of the photosynthetic apparatus in adverse environment, and it is considered as a protective reaction of P. pallasiana to the effects of drought.

Reaction of photosynthetic apparatus of a representative of extrazonal steppe plants Quercus robur to air pollution by motor vehicle emissions

We examined the vital condition of the common oak (Quercus robur L.) in plantations of different categories in Dnipro-city. In the investigated plantations, differences in the individual resistance of plants were recorded. The patterns of changes in the pigmented complex of leaves exposed to atmospheric pollution from motor vehicle emissions were studied in connection with the life state of oak trees. The content of chlorophyll a had, in all variants of the experiment, significantly higher values than chlorophyll b. Chlorophyll a accounted for 52.4–75.0% of the total content of chlorophylls, and chlorophyll b (25.0–47.6%). Regardless of the level of air pollution, relatively healthy oak plants were characterized by a high content of pigments activity in the photosynthetic apparatus of the leaves. A chronic moderate influence of motor vehicle emissions had a positive effect on the content of green pigments and carotenoids. Increase in the intensity of transport emissions resulted in a significant reduction in the accumulation of chlorophylls and to an increase in carotenoids. The stability of the chlorophyll a / chlorophyll b ratio under the influence of motor vehicle emissions was been registered. Only severely damaged common oak trees showed a decrease of this parameter by 29.3% compared with healthy and moderately weakened specimens of oak. The values of the coefficients of variation in the content of chlorophylls, their amounts and ratios, as well as carotenoids were low and represent less than 19.0%, while the amplitude of the variability of the studied parameters of the pigment complex, depending on the life state of plants and the intensity of transport emissions, was quite significant: the highest fluctuations in the сontent indexes were recorded for chlorophyll a, the sum of chlorophylls a + b in trees from the park area and the area with a medium level of pollution, chlorophyll b only in oaks from the street with an average level of air pollution by transport emissions. The smallest variability was a characteristic of the ratio of chlorophylls a / b and the content of carotenoids. Low values of the ratio of the sum of chlorophylls a + b to carotenoids (4.3–5.2) indicate the stress state of oak plants in urban environments.

Effect of lead on the photosynthetic apparatus of annual grasses

The effect of lead (in the form of Pb(CH3COO)2 x 3H2O at concentrations of 200, 400, and 800 mg/kg substrate) on the photosynthetic apparatus of barley (cultivar Dina) and oat (cultivar Falenskii) was studied. Exposure to low concentrations of lead (200 mg/kg) slightly increased the content of chlorophyll in leaves and the rate of photosynthesis. Medium concentrations of lead (400 mg/kg) had no marked effect on the parameters analyzed, whereas high concentrations (800 mg/kg) decreased the area of leaves, the content of green pigments in them, and the rate of photosynthesis.

Studies on Chlorophylls Contained in Vegetable Oils. I.

The composition and content of green pigments in alkali-refined rapeseed oil before and after bleaching were investigated.Pheophytins a and b were detected as major pigments, but chlorophylls a and b could hardly be found, when alkali-refined oil was fractionated by alumina column chromatography. The photofluorometric quantification of the same oil indicated essentially the same results.Bleaching tests of these four authentic pigments with activated clay as an adsorbent indicated the adsorption of each pigment to decrease in the following order: Chl a>Phy a>Chl b>Phy b. However, in experiments using an active carbon, no distinct difference in the adsorbed quantity of each pigments could be observed. The behavior of these pigments during the bleaching of rapeseed oil was analyzed photofluorometrically.

Studies on Greening of Canned Shellfishes

For the purpose of finding the seasonal variation of the content of green pigments in oysters at each oyster bed as below described, and also the relation between the content of green pigments and that of magnesium, zinc, iron, and copper, the oysters cultivated at Akitsu, Saka and Jigozen, along the coast of Hiroshima, were investigated from January 11 to June 10.Green pigments were estimated by measuring optical density at 660mμ for the solution which was extracted from oysters with hydrochloric acid-acetone (10:90) mixture. Iron was determined by 0-phenanthroline method, and magnesium, zinc and copper were determined by atomic absorption spectrophotometry.The content of green pigments in oysters considerably changes with bed or season. Although the content of these metals considerably changes with oyster bed, any remarkable seasonal change and also any definite interrelation between the content of metals and that of green pigments were not found.