Template For Construction Research Articles

Fabrication of Coffee‐Ring Nanostructured Membranes for Organic Solvent Nanofiltration

AbstractOrganic solvent nanofiltration (OSN) plays important roles in pharmaceutical ingredients purification and solvent recovery. However, the low organic solvent permeance under cross‐flow operation of OSN membrane hampers their industrial applications. Herein, we report the construction of coffee‐ring structured membrane featuring high OSN permeance. A water‐insoluble crystal monomer that dissolved in EtOH/H2O mixed solvent was designed to react with trimesoyl chloride via interfacial polymerization. Owing to the diffusion of EtOH to n‐hexane, coffee‐ring nanostructure on the support membrane appeared, which served as the template for construction of coffee‐ring structured membrane. The optimal nanostructured membrane demonstrated 2.6‐fold enhancement in the effective surface area with reduced membrane thickness. Resultantly, the membrane afforded a 2.7‐fold enhancement in organic solvent permeance, e.g., ~13 LMH/bar for MeOH, without sacrificing the rejection ability. Moreover, due to the rigid monomer structure, the fabricated membrane shows distinctive running stability in active pharmaceutical ingredients purification and the ability for concentration of medicines.

Fabrication of Coffee-Ring Nanostructured Membranes for Organic Solvent Nanofiltration.

Organic solvent nanofiltration (OSN) plays important roles in pharmaceutical ingredients purification and solvent recovery. However, the low organic solvent permeance under cross-flow operation of OSN membrane hampers their industrial applications. Herein, we report the construction of coffee-ring structured membrane featuring high OSN permeance. A water-insoluble crystal monomer that dissolved in EtOH/H2O mixed solvent was designed to react with trimesoyl chloride via interfacial polymerization. Owing to the diffusion of EtOH to n-hexane, coffee-ring nanostructure on the support membrane appeared, which served as the template for construction of coffee-ring structured membrane. The optimal nanostructured membrane demonstrated 2.6-fold enhancement in the effective surface area with reduced membrane thickness. Resultantly, the membrane afforded a 2.7-fold enhancement in organic solvent permeance, e.g., ~13 LMH/bar for MeOH, without sacrificing the rejection ability. Moreover, due to the rigid monomer structure, the fabricated membrane shows distinctive running stability in active pharmaceutical ingredients purification and the ability for concentration of medicines.

Research on data security governance of smart city construction

In the post-epidemic era, the construction of smart cities has become an important link in the economic development of various countries. To improve the degree of urban smart, accelerate the construction of urban digital information infrastructure, and promote the safe sharing of network data is the meaning of smart city construction. At present, China’s smart city construction has made breakthrough progress, but the data flow still faces problems such as leakage, abuse and sharing obstruction. Therefore, we can learn from the mature experience adopted by Europe and the United States in the construction of smart cities, set up third-party institutions to actively promote data sharing, build high-value data sets open to the public, and adhere to the first trial reform. The introduction of regulatory sandbox and common data space mechanism to improve the level of smart city construction and export China’s template for smart city construction.

Embodied semantic parameters for the lexical representation of spatial relational categories

Abstract This paper proposes an explanatory model for the lexical representation of the native speakers’ lexical knowledge of English prepositions. Lexical knowledge of prepositions as relational predicates includes argument structure (trajector-landmark) as in Cognitive Grammar, situation types (position vs state) as in Functional Grammar, lexical hierarchies (spatial subdomains) based on semantic primitives, as in Natural Semantic Metalanguage, and embodied perceptual parameters configured in four dimensions, namely, geometry, topology, force-dynamics and function (from Cognitive Linguistics). This model is illustrated here by expounding three lexical templates compatible with constructional templates in the Lexical Constructional Model, representing the semantic decomposition of English prepositions at, on and in.

Learning shared template representation with augmented feature for multi-object pose estimation

Template matching pose estimation methods based on deep learning have made significant advancements via metric learning or reconstruction learning. Existing approaches primarily build distinct template representation libraries (codebooks) from rendered images for each object, which complicate the training process and increase memory cost for multi-object tasks. Additionally, they struggle to effectively handle discrepancies between the distributions of training and test sets, particularly for occluded objects, resulting in suboptimal matching accuracy. In this study, we propose a shared template representation learning method with augmented semantic features to address these issues. Our method learns representations concurrently using metric and reconstruction learning as similarity constraints, and augments response of network to objects through semantic feature constraints for better generalization performance. Furthermore, rotation matrices serve as templates for codebook construction, leading to excellent matching accuracy compared to rendered images. Notably, it contributes to the effective decoupling of object categories and templates, necessitating the maintenance of only a shared codebook in multi-object pose estimation tasks. Extensive experiments on Linemod, Linemod-Occluded and TLESS datasets demonstrate that the proposed method employing shared templates achieves superior matching accuracy. Moreover, proposed method exhibits robustness on a collected aircraft dataset, further validating its efficacy.

Retraction: PEG-nanotube liquid crystals as templates for construction of surfactant-free gold nanorods.

Retraction of 'PEG-nanotube liquid crystals as templates for construction of surfactant-free gold nanorods' by Naohiro Kameta and Hidenobu Shiroishi, Chem. Commun., 2018, 54, 4665-4668, https://doi.org/10.1039/C8CC02013B.

Filamentary Dust Polarization and the Morphology of Neutral Hydrogen Structures

Filamentary structures in neutral hydrogen (H i) emission are well aligned with the interstellar magnetic field, so H i emission morphology can be used to construct templates that strongly correlate with measurements of polarized thermal dust emission. We explore how the quantification of filament morphology affects this correlation. We introduce a new implementation of the Rolling Hough Transform (RHT) using spherical harmonic convolutions, which enables efficient quantification of filamentary structure on the sphere. We use this Spherical RHT algorithm along with a Hessian-based method to construct H i-based polarization templates. We discuss improvements to each algorithm relative to similar implementations in the literature and compare their outputs. By exploring the parameter space of filament morphologies with the Spherical RHT, we find that the most informative H i structures for modeling the magnetic field structure are the thinnest resolved filaments. For this reason, we find a ∼10% enhancement in the B-mode correlation with polarized dust emission with higher-resolution H i observations. We demonstrate that certain interstellar morphologies can produce parity-violating signatures, i.e., nonzero TB and EB, even under the assumption that filaments are locally aligned with the magnetic field. Finally, we demonstrate that B modes from interstellar dust filaments are mostly affected by the topology of the filaments with respect to one another and their relative polarized intensities, whereas E modes are mostly sensitive to the shapes of individual filaments.

The CARMENES search for exoplanets around M dwarfs

Light from celestial objects interacts with the molecules of the Earth’s atmosphere, resulting in the production of telluric absorption lines in ground-based spectral data. Correcting for these lines, which strongly affect red and infrared wavelengths, is often needed in a wide variety of scientific applications. Here, we present the template division telluric modeling (TDTM) technique, a method for accurately removing telluric absorption lines in stars that exhibit numerous intrinsic features. Based on the Earth’s barycentric motion throughout the year, our approach is suited for disentangling telluric and stellar spectral components. By fitting a synthetic transmission model, telluric-free spectra are derived. We demonstrate the performance of the TDTM technique in correcting telluric contamination using a high-resolution optical spectral time series of the feature-rich M3.0 dwarf star Wolf 294 that was obtained with the CARMENES spectrograph. We apply the TDTM approach to the CARMENES survey sample, which consists of 382 targets encompassing 22 357 optical and 20 314 near-infrared spectra, to correct for telluric absorption. The corrected spectra are coadded to construct template spectra for each of our targets. This library of telluric-free, high signal-to-noise ratio, high-resolution (ℛ > 80 000) templates comprises the most comprehensive collection of spectral M-dwarf data available to date, both in terms of quantity and quality, and is available at the project website.

Remote Recruitment Strategy and Structured E-Parenting Support (STEPS) App: Feasibility and Usability Study.

The Structured E-Parenting Support (STEPS) app provides support for parents of children with elevated hyperactivity, impulsivity, inattention, and conduct problems who are awaiting clinical assessment. STEPS will be evaluated in a randomized controlled trial (RCT) within the Online Parent Training for the Initial Management of ADHD Referrals (OPTIMA) research program in the United Kingdom. Phase 1 of the OPTIMA tested the feasibility of participants' recruitment and the app's usability. This study aimed to adapt a digital routine clinical monitoring system, myHealthE, for research purposes to facilitate waitlist recruitment; test using remote methods to screen and identify participants quickly and systematically; pilot the acceptability of the recruitment and assessment protocol; and explore the usability of STEPS. myHealthE was adapted to screen patients' data. Parents' and clinicians' feedback on myHealthE was collected, and information governance reviews were conducted in clinical services planning to host the RCT. Potential participants for the observational feasibility study were identified from new referrals using myHealthE and non-myHealthE methods. Descriptive statistics were used to summarize the demographic and outcome variables. We estimated whether the recruitment rate would meet the planned RCT sample size requirement (n=352). In addition to the feasibility study participants, another group of parents was recruited to assess the STEPS usability. They completed the adapted System Usability Scale and responded to open-ended questions about the app, which were coded using the Enlight quality construct template. Overall, 124 potential participants were identified as eligible: 121 (97.6%) via myHealthE and 3 (2.4%) via non-myHealthE methods. In total, 107 parents were contacted, and 48 (44.9%) consented and were asked if, hypothetically, they would be willing to participate in the OPTIMA RCT. Of the 28 feasibility study participants who provided demographic data, 21 (75%) identified as White. Their children had an average age of 8.4 (SD 1.7) years and 65% (31/48) were male. During the primary recruitment period (June to July 2021) when 45 participants had consented, 38 (84%) participants agreed hypothetically to take part in the RCT (rate of 19/mo, 95% CI 13.5-26.1), meeting the stop-go criterion of 18 participants per month to proceed with the RCT. All parents were satisfied or very satisfied with the study procedures. Parents (n=12) recruited to assess STEPS' usability described it as easy to navigate and use and as having an attractive combination of colors and visual design. They described the content as useful, pitched at the right level, and sensitively presented. Suggested improvements included adding captions to videos or making the recorded reflections editable. Remote recruitment and study procedures for testing a parenting intervention app are feasible and acceptable for parents. The parents felt that STEPS was a useful and easy-to-use digital parenting support tool. RR2-10.1186/s40814-021-00959-0.

2073. Persistent HIV-1 Viremia Despite Intensive Antiviral Therapy Due to Non-responsive Clonal Viral Lineages

Abstract Background After initiation of antiviral therapy (ART), plasma HIV-1 RNA is usually undetectable after one month. In rare cases, viral suppression may not be achieved despite good adherence and with virus susceptible to ART. We used ultra-deep Primer ID next gen sequencing (NGS) to study the viral population and evolution of HIV-1 in two patients with persistent viremia on intensive ART. Methods We extracted viral RNA from plasma samples collected at multiple timepoints over the duration of the treatment from two patients (VEX1 and VEX2). We constructed Primer ID NGS libraries covering part of the pol gene and the env V1/V3 region and sequenced them on the Illumina MiSeq platform. We used the ‘tcs’ pipeline to construct template consensus sequences (TCS) for each region, and searched for drug resistance mutations (DRMs). The Geno2pheno pipeline was used to predict co-receptor tropisms. Results Patient VEX1 was followed for two years on ART. VEX2 restarted ART in the hospital and received directly observed therapy for nearly 6 months. Both had over 1 million viral copies/mL at the initiation of ART, and the CD4 cell counts were extremely low. Their viral loads slowly declined to approx. 10,000 copies/mL at the end of follow-up but complete viral suppression was not achieved for either patient despite appropriate ART. DRMs were not detected in either patient throughout the treatment with detection sensitivity as low as 0.1%. The sequencing results for VEX1 showed that there were both X4- and R5-tropic viruses at all timepoints and R5 viruses decayed much more slowly than X4 viruses, up to 35-fold more slowly in the initial 3 months of ART. Phylogenetic analysis revealed that the persistent R5 viruses were largely clonal while little clonality was found in the persistent X4 virus. In VEX2, all viruses were R5-tropic. There were three major distinct lineages in the viral population, and two of them completely disappeared after the initiation of ART while the other lineage persisted throughout therapy (Fig 1). The persistent lineage in VEX2 was highly clonal. Pooled maximum likelihood tree at env V1V3 region of 3 time points from patient VEX2. Sequences in red were from 2018 when we obtained the initial specimen from the patient. Sequences in blue were from Sept 2021 when the patient restarted ART. Sequences in green were from Oct 2021 when the patient were on ART for 3 weeks. The patient had 3 major viral lineages, L1, L2, and L3. From 2018 to Sept 2021, we could see the viral evolution of the three lineages (blue arrows). After 3 weeks of ART, L1 and L2 disappeared, while the L3 persisted. We could also see that L3 had several clonal populations which did not respond to ART nor evolve from 2018 to 2021. The additional sequences from Nov 2021 to Feb 2022 were identical to the sequences obtained on Oct 2021 (not shown on this figure). Conclusion Our study shows that persistent viremia on ART can come from clonal viral lineages that carry no DRMs. Clonally expanded and infected host cells might contribute to the phenomenon. Further study is needed to explore the origin of these clonal viral genomes. Disclosures Jonathan Parr, MD, Abbott Laboratories: Donation of laboratory testing and reagents|Gilead Sciences: Grant/Research Support|Virology Education: Honoraria|World Health Organization: Advisor/Consultant|World Health Organization: Grant/Research Support David A. Wohl, M.D., Gilead: Advisor/Consultant|Gilead: Grant/Research Support|Janssen: Advisor/Consultant|Lilly: Grant/Research Support|Merck: Grant/Research Support|ViiV: Advisor/Consultant|ViiV: Grant/Research Support Joseph J. Eron, MD, Adagio Therapeutics: data safety monitoring committee|Gilead Sciences: Advisor/Consultant|Gilead Sciences: Grant/Research Support|Glaxo Smith Kline: Advisor/Consultant|Merck: Advisor/Consultant|ViiV Healthcare: Advisor/Consultant|ViiV Healthcare: Grant/Research Support.

Study on Fine Management of Construction Engineering Projects Based on BIM Technology: Taking the Construction of Group C Project of Zibo Cultural Center as an Example

The roof steel structure of group C project of Zibo cultural center is composed of steel truss, welded H-shaped steel and large-diameter steel pipe embedded parts. The construction of embedded parts of inclined cylinder overhanging steel structure is difficult. The stair square tube ladder beam requires precise angles and radians, complex processing, and construction site setting out is difficult. A three-dimensional visual building information model has been established for this projects which includes several aspects which are as follows: the development of the post anchor construction technology of large steel sleeve embedded parts with inclined cylinders based on Building Information Modeling (BIM) technology, multi discipline synthesis and collision optimization, the net height optimization of electromechanical pipelines, fine reinforcement management, BIM virtual construction template. Through this planning, 14908 pipeline collisions, including 236 major collisions have been eliminated. BIM is used in handling and solving problems with the construction unit and the supervision company. This greatly improves the project work efficiency and saves meeting time. Through optimization of electromechanical line collision and secondary masonry layout, more than RMB 9 million has been saved in terms of personnel and materials, effectively reducing material losses, saving manpower, and significantly improving the implementation efficiency of the project. Based on the on-site simulation, the project planning scheme of the enterprise was adjusted, efficient work allocation with less idle time were ensured, and the correct utilization of project funds and time were ensured. The original extensive annual control and monthly control have been dynamically and accurately controlled to realize the dynamic understanding of the profits and losses of the project, and effectively reduce the occurrence of non-ideal situations.

Synergistic effect of cobalt in hierarchical carbon nitride nanorods (HCNNR) with promising charge transfer rate by hole scavenger for stimulating solar H2 production

One-dimensional (1D) hierarchical carbon nitrides nanorods (HCNNRs) mediated by cobalt was designed and synthesized using a template free hydrothermal approach for improving solar light assisted H2 evolution. The performance of 1D HCNNTs was significantly enhanced compared to bulk g-C3N4 due to larger active surface area, more visible light utilization and superior charge carrier along the unidirectional flow pathways. The 2% Co/HCNNRs (1D) exhibited excellent photocatalytic efficiency and optimal H2 yield reached up to 620 µmol g−1, which is 11.27 and 17.71 folds more than it was attained with pure HCNNR and bulk g-C3N4, respectively. This reveals that 1D structure helps to accelerate charge transport, whereas, Co works as a mediator to trap electrons, thus improving photocatalytic performance. The crucial operating parameters such as sacrificial reagents, feed concentration and catalyst loading were further optimized based on highest H2 evolution. Interestingly, mass transfer, charge transfer and amount of photoinduced charges were greatly dependent on the operating parameters. Higher methanol and lower TEOA concentration were beneficial to yield highest photoactivity and stability. This work provides new approach to construct template free 1D nanorods and would be beneficial to enhance performance in other solar energy related applications.

Sensitive chemoselectivity of cellulose nanocrystal films

Cellulose nanocrystals (CNCs), self-assembled into a chiral nematic structure film, create an advanced platform for the fabrication of remarkable sensing, photonic and chiral nematic materials. Despite extensive progress in the knowledge of functions of CNCs, their chemoselectivity has rarely been reported. Here, we report a brand-new perspective of CNCs in chemoselectivity, which shows sensitive selectivity even between isomers of monosaccharides and disaccharides by generating discernible crystal patterns. This sensitive selectivity of glucose homologs is attributed to the selective carbohydrate–carbohydrate interactions (CCIs) through generating hydrogen bonds between CNC units and the glucose homologs, endowing a remarkable color variation of the CNC films. Moreover, the CCIs are distinct for different immersion times and concentrations of glucose homologs, verified through Fourier Transform Infrared Spectroscopy (FTIR) spectra. Based on the sensitive CCIs, pristine CNC films are then used as templates to generate chiral mesoporous carbon films with tunable specific surface areas by assembly of CNC suspensions and the glucose homologs. We envision that the sensitive chemoselectivity of CNC films as well as precise structure modulation could provide insights into the recognition of carbohydrates and the preparation of mesoporous carbon in numerous practical applications. A pristine chiral nematic cellulose nanocrystal film with sensitive chemoselectivity to glucose homologues and even isomers of monosaccharides and disaccharide are prepared through self-assembly. For different kinds of glucose homologs, distinct crystal morphologies are formed due to the selective carbohydrate–carbohydrate interactions. Besides, the highly ordered left-helical layered structure of pristine cellulose nanocrystal film could be used as templates for the precise construction of delicate nanostructures.

A Deep Learning-Based Chinese Semantic Parser for the Almond Virtual Assistant.

Almond is an extendible open-source virtual assistant designed to help people access Internet services and IoT (Internet of Things) devices. Both are referred to as skills here. Service providers can easily enable their devices for Almond by defining proper APIs (Application Programming Interfaces) for ThingTalk in Thingpedia. ThingTalk is a virtual assistant programming language, and Thingpedia is an application encyclopedia. Almond uses a large neural network to translate user commands in natural language into ThingTalk programs. To obtain enough data for the training of the neural network, Genie was developed to synthesize pairs of user commands and corresponding ThingTalk programs based on a natural language template approach. In this work, we extended Genie to support Chinese. For 107 devices and 261 functions registered in Thingpedia, 649 Chinese primitive templates and 292 Chinese construct templates were analyzed and developed. Two models, seq2seq (sequence-to-sequence) and MQAN (multiple question answer network), were trained to translate user commands in Chinese into ThingTalk programs. Both models were evaluated, and the experiment results showed that MQAN outperformed seq2seq. The exact match, BLEU, and F1 token accuracy of MQAN were 0.7, 0.82, and 0.88, respectively. As a result, users could use Chinese in Almond to access Internet services and IoT devices registered in Thingpedia.

Hierarchical colloidosomes self-assembled from block copolymer micelles via emulsion interfacial confinement

Hierarchical self-assembly of polymeric building blocks into high-level colloidosomes is desirable to not only design novel nanostructures but also fabricate the complex artificial materials across many length scales with multifunctionality. Although great progress has been made in the designing the hierarchical colloidosomes, the fabrication of polymeric colloidosomes self-assembled from block copolymer (BCP) colloidal nanoparticles still remains challenge. Here, we report the fabrication of the hierarchical polymeric colloidosomes with typical hollow internal structures self-assembled from the polystyrene-block-poly (2-vinyl pyridine) (PS-b-P2VP) BCP spherical micelles through the emulsion interfacial confinement, which is constructed through the water-in-1-butanol emulsion system. Moreover, the hierarchical colloidosomes can disassemble into the original uniform spherical micelles under the acid aqueous solution, indicating that the colloidosomes possess good pH stimuli-responsibility. Finally, the stability of the colloidosomes can be greatly improved by cross-linking the P2VP corona of original spherical micelles, offering the effective templates for construction of the multifunctional materials. This finding provides a simple yet effective method for the fabrication of the hierarchical colloidosomes from the BCP building blocks.

Introduction to Rivera's Gender Affirming Nursing Care Model: A Middle-Range Theory.

Purpose: Currently, standards for transgender care guide surgical, hormonal, cosmetic and psychological care, yet do not include the domain of nursing care and nursing sensitive care outcomes. In response, the purpose of this project was to create a middle-range nursing theory to guide transgender nursing care. Methods: Rivera's Gender Affirming Nursing Care (GANC) Model was developed using a community informed iterative process, combining and modifying two existing theories. Each theory was evaluated, construct templates were created, modified, and expanded to create the proposed gender transcendent model. Face validity was established through expert and community review. Findings: Concepts identified include: Relationship (Join With), Knowledge (Learn With) and Engagement (Partner With). Action within the model is propelled by drivers, including ongoing learning, ongoing research, ongoing self-assessment, and leadership. The model operates within the recognized domains of nursing, to support gender affirming nursing care. Conclusions: The middle-range theory was tested and found to stimulate gender neutral thinking; however, the model would benefit from additional testing to determine impact on nursing and patient outcomes. The model allows the nurse, through self-reflection and other internal growth mechanisms, to identify personal implicit and explicit bias. It is through these actions and shifting paradigms that nurses develop a personal gender affirming nursing practice.

Sperm Repository for a Breeding Program of the Eastern Oyster Crassostrea virginica: Sample Collection, Processing, Cryopreservation, and Data Management Plan.

Simple SummaryThe Eastern oyster Crassostrea virginica is one of the most important fishery and aquaculture species in the USA and is a keystone species for coastal reefs. A breeding program was initiated in 2019 to support the fast-growing aquaculture industry for this species in the Gulf of Mexico. Oysters from wild populations in embayment along the U.S. Gulf of Mexico coast were used as broodstock for the program to maximize genetic diversity. A sperm repository of the broodstock, including a total of 102 male oysters from the 17 collection sites, was established to support the breeding project. Sperm collection was accomplished by strip spawn, and fresh sperm production, motility, and fertility were recorded for quality analysis. Cryopreserved sperm samples were sorted, labelled, archived, and stored in liquid nitrogen for future use. Post-thaw motility and plasm membrane integrity were recorded as post-thaw quality parameters. Overall, this study demonstrated sperm sample collection, processing, cryopreservation, and a data management plan involved in the establishment of the sperm repository. The streamlined procedure can serve as a template for construction of oyster germplasm repositories for breeding programs.The Eastern oyster Crassostrea virginica (Family Ostreidae) is one of the most important fishery and aquaculture species in the U.S. and is a keystone species for coastal reefs. A breeding program was initiated in 2019 to support the fast-growing aquaculture industry culturing this species in the Gulf of Mexico. Oysters from 17 wild populations in embayment along the U.S. Gulf of Mexico coast from southwest Florida to the Matagorda Bay, Texas were used as broodstock for the program to maximize genetic diversity in the base population. A sperm repository of the broodstock was established to support the breeding project. The goal of this study was to demonstrate the sperm sample collection, processing, cryopreservation, and the data management plan involved in the establishment of a sperm germplasm repository of base populations. The supporting objectives were to: (1) develop a data management plan for the sperm repository; (2) streamline the procedure for sample collection, processing, and cryopreservation; (3) incorporate sperm quality analysis into the procedure, and (4) archive the cryopreserved samples as a repository for future use in the breeding program. This sperm repository included a total of 102 male oysters from the 17 collection sites (six oysters per site). A data management plan was developed with six categories, including sample collection, phenotype, fresh sperm, genotype, cryopreservation, and post-thaw sperm, as guide for data collection. Sperm collection was accomplished by strip spawn, and fresh sperm production, motility, and fertility were recorded for quality analysis. Cryopreserved sperm samples were sorted, labelled, archived, and stored in liquid nitrogen for future use. Post-thaw motility (1–30%) and plasm membrane integrity (15.34–70.36%) were recorded as post-thaw quality parameters. Overall, this study demonstrated a streamlined procedure of oyster sperm collection, processing, and cryopreservation for establishing a sperm repository that can serve as a template for construction of oyster germplasm repositories for breeding programs.

Light-Controlled Friction by Carboxylic Azobenzene Molecular Self-Assembly Layers.

Nowadays, reversible friction regulation has become the focus of scientists in terms of the flexible regulatory structure of photosensitive materials and theories since this facilitates rapid development in this field. Meanwhile, as an external stimulus, light possesses great potential and advantages in spatiotemporal control and remote triggering. In this work, we demonstrated two photo-isomerized organic molecular layers, tetra-carboxylic azobenzene (NN4A) and dicarboxylic azobenzene (NN2A), which were selected to construct template networks on the surface of the highly oriented pyrolytic graphite (HOPG) to study the friction properties, corresponding to the arrangement structure of self-assembled layers under light regulation. First of all, the morphology of the self-assembled layers were characterized by a scanning tunneling microscope (STM), then the nanotribological properties of the template networks were measured by atomic force microscope (AFM). Their friction coefficients are respectively changed by about 0.6 and 2.3 times under light control. The density functional theory (DFT) method was used to calculate the relationship between the force intensity and the friction characteristics of the self-assembled systems under light regulation. Herein, the use of external light stimulus plays a significant role in regulating the friction properties of the interface of the nanometer, hopefully serving as a fundamental basis for further light-controlling research for the future fabrication of advanced on-surface devices.

RNA nanotechnology to build a dodecahedral genome of single-stranded RNA virus

ABSTRACT The quest for artificial RNA viral complexes with authentic structure while being non-replicative is on its way for the development of viral vaccines. RNA viruses contain capsid proteins that interact with the genome during morphogenesis. The sequence and properties of the protein and genome determine the structure of the virus. For example, the Pariacoto virus ssRNA genome assembles into a dodecahedron. Virus-inspired nanotechnology has progressed remarkably due to the unique structural and functional properties of viruses, which can inspire the design of novel nanomaterials. RNA is a programmable biopolymer able to self-assemble sophisticated 3D structures with rich functionalities. RNA dodecahedrons mimicking the Pariacoto virus quasi-icosahedral genome structures were constructed from both native and 2ʹ-F modified RNA oligos. The RNA dodecahedron easily self-assembled using the stable pRNA three-way junction of bacteriophage phi29 as building blocks. The RNA dodecahedron cage was further characterized by cryo-electron microscopy and atomic force microscopy, confirming the spontaneous and homogenous formation of the RNA cage. The reported RNA dodecahedron cage will likely provide further studies on the mechanisms of interaction of the capsid protein with the viral genome while providing a template for further construction of the viral RNA scaffold to add capsid proteins for the assembly of the viral nucleocapsid as a model. Understanding the self-assembly and RNA folding of this RNA cage may offer new insights into the 3D organization of viral RNA genomes. The reported RNA cage also has the potential to be explored as a novel virus-inspired nanocarrier.

Of absent mothers, strong sisters and peculiar daughters: The constructional network of English NPN constructions

Abstract This paper analyzes symmetric NPN constructions (e.g., day to day, face to face, step by step) qualitatively and quantitatively by examining data from the Corpus of Contemporary American English (Davies, Mark. 2008–. The Corpus of Contemporary American English (COCA): 570 million words, 1990–present. http://corpus.byu.edu/coca/). The constructions’ frequency and productivity, as well as their semantics and extension potential (i.e., modification, complementation) is investigated (e.g., by conducting collostructional analysis). In terms of theoretical modeling, the paper takes a Usage-based, Cognitive Construction Grammar approach (UCCxG) and sketches the constructional network of this constructional family, postulating various constructional templates on different levels of specificity – among others – the existence of the following subtypes [CNsg,time i after CNsg,time i]Cx (e.g., day after day, night after night), [CNsg,measurement i by CNsg,measurement i]Cx (e.g., inch by inch, step by step) or [CNsg,bodypart i to CNsg,bodypart i]Cx (e.g., skin to skin, shoulder to shoulder). We show how these templates are vertically and horizontally connected to each other. Ultimately, we argue that in a usage-based model which strives for cognitive plausibility it is not always feasible to postulate the entrenchment of an abstract overarching schema (i.e., a ‘mothernode’) like [CNi P CNi]Cx or even [N P N]Cx high up in the network. It is unlikely that speakers abstract such a general schema in a bottom-up acquisition process for this family. Rather, the NPN group is a constructional family characterized by many sister ties and by the absence of mother nodes from which information can be inherited.