Connective Tissue Septa Research Articles

Correlation between the expression of the iNOS, caspase-3 and α-SMA genes in the parotid glands of albino rats following the administration of two antihistamines from two different generations.

Several medications, including antihistamines, can alter salivary gland function, causing dry mouth or xerostomia. Antihistamines are commonly used for treating allergic rhinitis. The aim of the present study was to compare and correlate the effects of first-generation vs. second-generation H1-antihistamines on the parotid glands of rats. Twelve adult male albino rats were used; 4 rats served as a control group (group I) and the remaining rats were divided into 2 groups: group II received promethazine hydrochloride; and group III received cetirizine dihydrochloride for 3 weeks. The parotid salivary glands were dissected, and examined histologically and analyzed histomorphometrically for the acinar area percentage. In addition, mRNA gene expression of iNOS, caspase-3 and α-SMA was assessed using quantitative realtime polymerase chain reaction (qRT-PCR). Finally, all the obtained data was statistically analyzed. Histologically, group I showed the typical architecture of the gland. In group II, degenerative changes were noticed, including acinar degeneration and shrinkage with widened connective tissue septa, intracellular vacuolization, and increased inflammatory cell infiltration. In group III, similar histological features were detected as in group II, but to a lesser extent. Histomorphometric results revealed significant differences in the acinar area percentage between various groups. In addition, qRT-PCR results showed a significant increase in iNOS expression in both groups II and III as compared to group I, caspase-3 gene expression was significantly increased in group II, while in group III, it increased non-significantly. Finally, α-SMA gene expression non-significantly decreased in both groups II and III. A significant positive correlation was observed between caspase-3 and iNOS gene expression, while an inverse correlation was noticed between caspase-3 and α-SMA gene expression. The administration of antihistamines resulted in changes in the rat salivary glands, which could be due to the induction of oxidative stress and the resultant apoptotic effect. These changes were suggested to occur mainly through action on muscarinic receptors; yet, action on histamine receptors could not be excluded. However; these effects were less marked with the second-generation antihistamine.

Study of the localization of pigment cells in the skin of scaly carp of various ages (Cyprinus carpio L.)

Morphological methods of fish research provide an opportunity to study their biological systems – cells, tissues, and organs in normal and pathological conditions – in depth at all levels of structural organization. The work aimed to investigate the morphological features of localization of skin pigment cells in scaly carp (Cyprinus carpio L.) of different ages. The study was conducted in the laboratory of complex ichthyopathological studies at the Department of Ichthyology and Zoology of the Belotserkiv National Agrarian University. One-, two-, and three-year-old scaly carp from the feeding ponds of PJSC “Chernigivrybhosp” were used. From which the scales were removed, skin samples of scaly carp (Cyprinus carpio L.) were used as material for research. Tissue samples were taken from freshly caught fish for the research. Our study established that pigment cells in carp of different ages were localized in the upper and lower layers of the dermis and hypodermis and in the lateral connective tissue septum at the level of the lateral line that divides the lateral muscles. In addition, in the carp of the same age, in contrast to the older age groups, isolated clusters of pigment cells were observed in the loose connective tissue between the muscle fibers of the superficial lateral muscle. During the histological examination, we also discovered the presence of pigment cells in the skin, particularly melanophores. Thus, the skin of peers of scaly carp was located in single clusters and had a rounded shape and a concentrated distribution of melanin pigment. Many melanophores were observed in the lower layer of the dermis at the border with the hypodermis. In the area of the lateral line, in the connective tissue septum, at the level of the deep lateral muscle, large strands of melanophores were detected.

Anatomical and Functional Study of the Ostrich (Struthio camelus) Lung through Macroscopic Analysis in Combination with Optical and Electron Microscopy Techniques.

The Ostrich occupies a unique position as the largest bird on the planet. Like other ratites, it has been reputed to have a phylogenetically primitive lung. We used macroscopy, light microscopy, transmission and scanning electron microscopy as well as silicon rubber casting to elucidate the functional design of its lung and compare it with what is already documented for the avian species. The neopulmonic region was very small and poorly developed. The categories of the secondary bronchi (SB) present and their respective numbers included laterodorsal (8-10), lateroventral (4-5), medioventral (4-6) and posterior (16-24). The lateral aspects of the laterodorsals were covered with a transparent collapsible membrane internally lined with a squamous to cuboidal epithelium. The bulk of these SB were in close proximity to intercostal spaces and the intercostal muscles and were thought to be important in the propulsion of gases. The lung parenchyma was rigid, with the atria well supported by septa containing smooth muscles, connective tissue interparabronchial septa were absent, and blood capillaries were supported by epithelial bridges. There were two categories of epithelia bridges: the homogenous squamous type comprising two leaflets of type I cells and the heterogeneous type consisting of a type I pneumocyte and type II cell. Additional type two cells were found at the atrial openings as well as the walls of the infundibulae and the air capillaries. The atria were shallow and opened either directly into several air capillaries or into a few infundibulae. The presence of numerous type II cells and the absence of interparabronchial connective tissue septa may imply that the ostrich lung could be capable of some degree of compliance.

Embryonal Rhabdomyosarcoma: Rare Case Reports with Middle Ear Location

Background: Patients with embryonal rhabdomyosarcoma (EMRS) are dominated by boys. The peak incidence occurs in the small age group from one year to 4 years. Approximately 35% of all pediatric RMS occur in the head and neck.
 Case presentation: A 3-year-old male patient came to the ear, nose, throat-head neck (ENT-HNS) polyclinic at Dr. M. Djamil General Hospital Padang on February 25th, 2021, with complaints of a lump appearing in his right ear canal for 4 months. The patient also complained of slanting on the right side of his face and pain in his right ear since 2 weeks ago. History of bleeding from the ear 1 week ago. On the examination of House-Brackmann, the patient obtained a category House-Brackmann V. Histopathological results, microscopically visible pieces of tissue with part of the surface covered with keratinized squamous epithelium, part of the surface not keratinized. There are dense groups consisting of a proliferation of cells with round, oval nuclei, small to medium size, little cytoplasm, some cells with pleomorphic nuclei, hyperchromatic, irregular nuclear membranes, lots of eosinophilic cytoplasms, with the appearance of "tadpole”, atypical mitoses may be found. The underlying tissue stroma appears myxoid. Among them, fibrotic connective tissue septa containing partially hyperemic capillaries are visible. Necrotic areas and clusters of PMN leukocytes and cellular debris were also seen. This description is consistent with EMRS.
 Conclusion: The patient was diagnosed with right middle ear EMRS stage III with right peripheral facial nerve paresis House-Brackmann V.

Role of CD68+ and CD206+ cells in the progression of toxic liver fibrosis in rats

The aim of the work was to evaluate the role of stellate macrophages in a large number of points of toxic liver fibrosis in rats. Liver fibrosis and cirrhosis in male Wistar rats were induced with thioacetamide at a dose of 200 mg/kg animal weight for 17 weeks. Histological preparations of the liver were stained with hematoxylin and eosin according to the Mallory method. Immunohistochemical examination was performed on paraffin sections using monoclonal mouse antibodies CD68 and polyclonal rabbit antibodies CD206. The fibrosis degree was determined according to the Ishak semi-quantitative scale. Toxic liver fibrosis before the start of its transformation into cirrhosis (9 weeks) was accompanied by an increase in the number of CD68+ cells compared with the control. At all subsequent experiment stages, no differences were found in comparison to the control. In the liver of control rats, CD206+ cells were practically absent. Throughout the experiment, their number remained above the control point – 3 weeks. With the progression of liver cirrhosis, a decrease in the number of CD206+ cells was noted, but it did not reach a level of 3 weeks. Morphologically, two different groups of CD68+ cells were identified. One group of cells had a pterygoid shape and they were located mainly in the liver sinusoids. The second group of CD68+ cells had a round shape and different localization. They were detected around the vessels of portal zones, surrounded brown pigment accumulations in connective tissue septa, were observed near single lying groups or groups of giant hepatocytes and liver cells containing brown pigment in the cytoplasm, and were also noted in the foci of necrosis of hepatocytes. Cells, expressing the CD206 marker, are round in shape and are elongated and located in the liver sinusoids. Presumably, round-shaped CD68+ cells perform a phagocytic function, and pterygoid-shaped CD68+ cells transdifferentiate into CD206+ cells that have anti-inflammatory properties.

Immunohistochemical study of human pineal vessels

BACKGROUND: The pineal gland is a neuroendocrine organ located in the epithalamic area of the brain. By using the melatonin, a pineal hormone, the pineal gland synchronizes the work of the internal physiological systems of the body with the circadian light-darkness cycle. Melatonin is synthesized in pinealocytes, the endocrine cells of the pineal gland, and secreted into the bloodstream. However, the structural features of the blood vessels in the pineal gland are still not well understood.
 AIM: The purpose of this study was to elucidate the intraorgan localization and immunohistochemical pattern of the blood vessels of the pineal gland of human, which had not been previously studied.
 MATERIALS AND METHODS: In the research, immunohistochemistry methods were applied using two selective markers of blood vessels, the antibodies to von Willebrand factor and type IV collagen. Von Willebrand factor is expressed selectively in endothelial cells that form blood vessels, including small capillaries, while type IV collagen is inherent to the basement membrane that separates the vascular endothelium from the underlying tissue.
 RESULTS: The immunohistochemical reaction to both markers clearly visualize the blood vessels of the human pineal gland, which in both cases were observed mainly in the connective tissue septa (trabeculae), or, in the absence of a regular lobular structure, in the connective tissue layers. In lobules surrounded by connective tissue trabeculae and containing a large number of densely packed pinealocytes, von Willebrand factor- and type IV collagen-immunoreactive structures were very rare, and in many cases were not observed. The found phenomenon of distribution of blood vessels in the human pineal gland is described for the first time.
 CONCLUSIONS: Since blood vessel markers with well-proven selectivity were used, the results obtained with their usage can be considered reliable; this gives grounds with a high degree of probability to assert that the majority of pinealocytes in the human pineal gland do not have direct contact with blood vessels and, accordingly, cannot secrete melatonin directly into the bloodstream. On the basis of the results obtained, a hypothesis is proposed that the hormone secretion from pinealocytes into blood vessels is mediated by astroglial cells.

Morphological description of the pancreas in male and female swan geese

One of the organs that can be found in the abdominal cavity is called the pancreas. It is a vital component in the process by which the food we eat is transformed into fuel for the cells of the body. For investigation of morphological description of the pancreas, the study was performed on twenty adult swan geese (10 males and 10 females). According to the morphological findings, the pancreas of geese was located on the right side of their abdominal cavity. It possessed four lobes—dorsal, splenic, ventral, and third and a distinctive duodenal papilla. It was between the ascending and descending duodenal loops. The goose pancreas has a thin covering of connective tissue over its mesothelium. The pancreatic parenchyma was divided by connective tissue septa, into numerous lobules, and these lobules extended outward from the pancreatic capsule. There was a wide range of diameters for the blood vessels. It consists of significant blood arteries, which can be identified by their oval or circular, bright nuclei. They have oval or circular, brilliant nuclei with the nucleolus. In the swan goose, serous acini and islets of Langerhans made up most of the pancreatic parenchyma. Exocrine cells had pyramidal shapes, acidophilic granules, and serous acini. In conclusion, the pancreas of geese like the most bird and is different from domestic animals.

Aneurysmal bone cyst of dorsal spine presenting with paraparesis in a pediatric patient: a case report

Introduction: Aneurysmal bone cyst (ABC) are rare benign tumours of spine. They most commonly presents with backache. However neurological deficit in spinal ABCs are not uncommon. Magnetic resonance imaging of ABC may sometimes be confused with osteosarcoma or Giant cell tumours. Treatment options are varied but complete excision of tumour with spine stabilisation should be the goal. Clinical case: Here we presented the case of a 13 year old girl presenting with spastic paraparesis and bladder incontinence with MRI dorsal spine suggestive of an expansile lesion of D5 body. She underwent subtotal corpectomy with fixation of spine . Biopsy was suggestive of aneurysmal bone cyst. Post operative neurological outcome was excellent and followup scans over 2 year period suggest complete remission. Discussion: ABC are non neoplastic expansile bone lesion consisting of blood filled spaces separated by connective tissue septa of bony trabeculation and osteoclastic giant cells. Similar to our case it is seen more commonly in young females. Lumbar spine has higher incidence of ABC as compared to dorsal and cervical spine. Most common presentation is backache. Surgical options for ABC include intralesional curettage, en bloc resection followed by fixation. Vertebroplasty is also a treatment option to reinforce spinal stability. Post operative radiotherapy also can be considered in partially resected cases. Although ABC have high recurrence rate of 10-44% which has been reported with 2 years following surgery. However our patients continue to have no radiological evidence of recurrence on 2 year follow up. Conclusion: ABCs are benign tumour which may present with neurological deficit. Although treatment of ABC remains controversial but early surgery with complete removal of tumour in patients with neurological deficit provides excellent recovery. However due to high recurrence rate regular follow up is necessary.

Three-dimensional analysis of junctions between efferent and epididymal ducts in the human caput epididymis.

Due to the scarcity of studies using human tissues, the limited information is currently available on the gross structure of the caput epididymis in humans, at which efferent ducts connect to the epididymal duct. The present study investigated the three-dimensional structures of efferent and caput epididymal ducts in humans, with a focus on junctions between the former and the latter. We examined three sets of human efferent and caput epididymal ducts in specimens obtained from prostatic carcinoma patients. They were reconstructed from serial paraffin sections using a segmentation model created by a deep learning protocol and high-performance three-dimensional reconstruction software. Serial sections and three-dimensional images of human efferent and caput epididymal ducts were combined to obtain the detailed anatomical information. When a single efferent duct was defined as a duct connecting to both the extra-testicular rete testis and epididymal duct, there were 14.7 efferent ducts with a total length of 3.0m per specimen on average. The cranial portion of the efferent ducts joined to a single duct and terminated at the end of the epididymal duct, whereas other efferent ducts terminated independently on the side of the epididymal duct. These two types of junctions between the efferent and epididymal ducts differed in the patterns of the epithelial-type switch. The epididymal duct consisted of multiple segments, which were separated by a minimal amount of connective tissue septa or even without them. Efferent ducts occupied most of the volume of the caput epididymis. By utilizing deep learning, we reconstructed human efferent and caput epididymal ducts and revealed their precise three-dimensional structures, which differed from those of rodents in several aspects. The present results may be useful for analyzing anatomical abnormalities related to some types of male infertility.

Histogenesis of Parotid Gland in Human Fetuses

Background: The present study attempted to find out the histological changes of parotid gland in the developing human fetuses. Materials and Methods: Parotid glands from 60 fresh fetuses of gestational weeks ranging from 12weeks to term were studied after staining with Hematoxylene & Eosin, Masson’s Trichome stain, Van Gieson’s stain and Verhoeff’s stains. Results: The gland of the fetus at 12 weeks composed of solid epithelial cords with occasional canalization, surrounded by loose mesenchyme. Canalization of cords was completed at 25 weeks and adult picture attained at 36 weeks. Division of the glandular parenchyma into lobes and lobules by connective tissue septa started at 17 weeks. A well formed capsule was seen around the gland at 20 weeks. A gradual decrease in intra-glandular connective tissue occurred in late gestational age. Variety of cells such as fibroblasts, mesenchymal cells, fibrocytes and lymphocytes were seen. Numerous adipocytes were found within the glandular parenchyma and around the gland. Ductal tree showed gradual differentiation, presence of cilia-like surface projections from epithelia of larger ducts were seen in 14-32 week fetuses. Conclusion: When compared with various authors, some findings of the present study followed the foot-steps of previous workers whereas some were in contrary, the most important difference being the age of full maturation of the glandular architecture. One of the unique finding being the presence of cilia-like surface projections seen in the larger ducts in 14-32 week aged fetuses.

The equine patellar ligaments and the infrapatellar fat pad – a microanatomical study

BackgroundInterpretation of patellar ligament (PL) ultrasonography may be difficult, as hypoechoic or heterogenous echogenicity are common findings. Verifying suspected disease of equine PLs by histopathology is also problematic as descriptions of normal PL vascularity and histology are scarce. The current study describes the PL and infrapatellar fat pad (IFP) vascular pattern from computed tomography scans of barium perfused normal equine specimens (n = 8; age 10 days to 18 years), as well as routine histology to serve as a reference for future investigations into PL pathology and IFP disease.ResultsThe PLs received a bipolar blood supply. Vascular architecture consisted of numerous distinct longitudinal vessels with several horizontal connections, which branched into extensive latticeworks of smaller vessels throughout the ligaments. Several vascular connections between the PLs and the IFP were identified. One distinct longitudinal vessel was seen entering each of the IFP lobes at the distocranial aspect, branching extensively into lobar vascular networks which anastomosed by several horizontal branches at the mid portion of the IFP where the two lobes merge. Histologically, there were large variations in PL interfascicular endotenon thickness, vascularity and fatty infiltration; these parameters increased with age for the intermediate and medial PL. Areas of metaplastic tenocytes / chondroid metaplasia were identified in all investigated adult medial PLs; in 2/7 in the intermediate PL and in 4/7 in the lateral PL. The adult IFP consisted of white unilocular adipose tissue, organized in lobules separated by thin connective tissue septa increasing in thickness towards the periphery and the distocentral aspect.ConclusionsThe equine PLs and IFP are highly vascularized structures with ample vascular connections suggestive of crosstalk. This, together with the large variation in PL endotenon thickness, vascularity and fatty infiltration, should be taken into consideration when assessing potential PL histopathology as these changes increase with age and are found in horses without clinical signs of stifle disease. Metaplastic tenocytes / chondroid metaplasia should be considered a normal finding throughout the medial PL and is not age dependent. The role of the equine IFP in stifle disease has yet to be elucidated.

Morphometrical and Histochemical study of glandular stomach (Proventriculus) in local domestic male ducks (Anas Platyrhynchos)

The present work explores morphometric and histochemical foundations of the wall of proventriculus in adult domestic male ducks. To achieve this study, six healthy adult ducks were purchased from local vendors in Mosul province. All birds were sedated. Their stomachs were removed after being dissected to achieve the gross description and measurements of the proventriculus, including the length and diameter of cranial, middle, and caudal regions of the proventriculus. Small tissue pieces 6-8 mm in length from three regions of proventriculus were processed for histomorphological studies using hematoxylin and Eosin stain, combined PAS-AB pH 2.5, toluidine blue, and Masson's trichrome stains. The finding showed that the proventriculus connects with the esophagus from the cranial side and the muscular stomach from the caudal side. It was a thin elongated oval tubular organ. Histologically, the mucosa of the proventriculus revealed the presence of folds and sulci-like structures covered by simple columnar epithelium. The tunica submucosa consisted of many signs of profound and straightforward sub-mucosal glands supported by connective tissue septa and extended from the basal part of the diverged gland through the lamina propria, which was organized in a conical manner (adenomere). Tunica Muscularis appeared as a double-layer coordinated in the form of inner longitudinal and outer circular ones. Finally, serosa consists of fibrous tissue, vessels, fatty tissue, and nerve plexuses studded by the mesothelial cells. The highest thickness of the four layers of mucosal, submucosal, muscular, and serosal layers in the distal part of ‎the proventriculus. Histochemical PAS staining positively reacted with epithelial tunica mucosa and wall of proventricular glands.

The Antioxidant Impact of Supplementing High and Low doses of Ashwagandha Extract in Chronic Pancreatitis Induced Experimentally

The pancreas is part of the gastrointestinal system that lies in the upper abdomen behind the stomach. It is a mixed endocrine/exocrine gland enveloped by a thin layer of connective tissue. Connective tissue septa invade the gland, dividing it into lobules, serving as a support for tissue and providing a pathway for vasculature, nerve fibers, and lymphatic ducts. The pancreas lacks a complete fibrous capsule (Abozaid et al., 2020). The pancreas is composed of exocrine portion which comprises more than 95% of the pancreatic mass and it responsible for makes and secretes digestive enzymes into the intestine and endocrine portion (the islets) which makes and secretes hormones as (insulin, glucagon, somatostatin and pancreatic polypeptide) into the blood to control energy metabolism and storage throughout the body, it includes acinar and duct cells with associated connective tissue, vessels, and nerves.it comprise 1-2% of pancreatic mass Mederos et al (2021).

Morphometric parameters of sinusoidal capillaries, interlobular veins and interlobular arteries at different stages of experimental liver fibrosis

The aim of the study was to conduct a morphometric analysis of sinusoidal capillaries, interlobular veins and interlobular arteries of the liver at different stages of experimental fibrosis in order to investigate relationship between fibrogenesis and angiogenesis and identify new therapeutic targets. Material and methods. The study included male Wistar rats. Liver fibrosis with transformation into cirrhosis was induced with a solution of thioacetamide, which was injected into the stomach, dosage 200 mg/kg of animal body weight, using a probe 2 times a week. The rats of the experimental groups were withdrawn from the experiment in 3, 5, 7, 9, 11, 13, 15, and 17 weeks, and the intact ones - at the end of the experiment. To identify connective tissue, liver sections were stained according to the Mallory method. Immunohistochemical examination was performed on paraffin sections using a monoclonal mouse antibody to CD31. The number of sinusoidal capillaries, interlobular arteries, and interlobular veins was counted in three non-overlapping fields of view of each histological section using an OLYMPUS BX51 microscope at a 40× objective magnification. Results. The results obtained supported a statistically significant decreased number of sinusoidal capillaries (p=0,0000) and an increased number of interlobular veins (p=0,0000) in animals of the experimental group compared with the control group. There were no differences in the number of interlobular arteries (p=0,9999). Pronounced angiogenesis in the portal zones and connective tissue septa was associated with an increased amount of the connective tissue during liver fibrogenesis. Conclusion. In the future, angiogenesis modulation should be differentiated and directed towards antifibrotic, anti-inflammatory or autoimmune therapy in chronic liver diseases.

Histomorphometrical and histochemical study of the pancreas on the local dogs (Canis lupus familiaris)

In the current study, ten adult healthy local dogs of both sexes were used to perform histomorphometrical study on their pancreases. The dog pancreas had an inverted V-shape consisting of left and right lobes joined by a body. The pancreas possesses both exocrine and endocrine parts. The exocrine portion was composed of numerous acini and fewer tubules as well as the duct system. The number, total diameter and cellular height of acini were significantly abundant, larger and taller in the right lobe compared those of the body and left lobe. Furthermore, the number and total diameter of large pancreatic islets were significantly abundant and larger in the left lobe despite with those of the body and right lobe. This result leads to consider the right lobe was a target region for enzymatic secretion, while the left lobe has a specific function for hormonal secretion. According to available literature, no such result and thus conclusion had been reported on dog pancreas previously. In all parts of the pancreas and with aldehyde fuchsin stain, only the large pancreatic islets contained α-, β-, δ-cells as well as non-staining cells. Only, the β-cells occurred as single cells between the pancreatic acini or even within the connective tissue septa. These single cells were more numerous in the left lobe, but they were infrequently located in the body and right lobe.

Ameliorative effect of different mesoporous bioactive glass materials in experimental tibial defects in rats.

Enhancing the bone healing procedure would resultantly improve the post-recovery life quality, as well as the speed with which the patient returns to their former life quality. Porous structures can provide a large surface area and abundant channels to facilitate mass transfer. To evaluate the application of mesoporous materials in the bone healing of surgically created defects on the tibiae of male adult Wistar rats. The defect areas were evaluated after implantation of 4 types of bioactive glass histopathologically and immunohistochemically. Fifty adult rats were divided into 5 groups including a control group without material. The used products were mesoporous bioactive glass (MBG), Cu-MBG, Zn-MBG, and Cu-Zn-MBG. Unicortical bone defects with a 3 mm diameter were performed in both tibiae of the animals and filled with 4 types of glass particles. The rats were then euthanized at 15 d and 30 d. Tibial samples were collected and the tissues forwarded for histological processing, and examined using light microscopy. Additionally, bone healing was evaluated by assessing the levels of bone morphogenetic protein BMP2, collagen 1, osteocalcin (OST), and vascular endothelial growth factor (VEGF) using immunohistochemical methods. Within the 15th day, all groups presented connective tissue septa; at the 30th day, the new bone formation was more intense in the Cu-Zn-MBG group. Additionally, BMP2, collagen 1, OST, and VEGF immune expression were more prominent in the Cu-Zn-MBG group. The study results indicated that MBG may be used for the repairing of bone defects. Cu-Zn-MBG may be the best choice for this purpose.

Morphological examination of the accessory sex glands of the Barki bucks (Capra hircus).

The present investigation was prepared to describe the accessory sex glands of the Barki bucks grossly and by light microscopy. There are four sex glands: ampullary, vesicular, prostate, and bulbourethral. The ampullary gland is an enlargement of the terminal part of the ductus deferens, its glandular part has branched tubuloalveolar glands, and its secretory alveoli lined with a pseudo-stratified epithelium composed of cuboidal to columnar cells. The vesicular gland takes the appearance of a cluster of grapes and the left vesicular gland is enlarged and higher than the right one. The vesicular gland is a lobulated tubuloalveolar gland with wide intralobular space and the gland contain a secretory unit which lined by pseudo-stratified columnar epithelium, and the interlobular ductules lined by the stratified epithelium, while the interlobular duct lined by simple cuboidal epithelium; moreover, the lining epithelium of secretory part consists of tall columnar cells. The prostate gland consists only of the disseminated part and is enclosed by a connective tissue capsule that was thin dorsally, thick laterally, and reduced in thickness ventrally. The prostatic acini are lined by simple cuboidal epithelium. The bulbourethral gland was similar in size to the walnut and surrounded by a capsule and there are interlobular connective tissue septa that divided the gland into lobes and lobules of different sizes. The bulbourethral gland contained secretory units lined by the tall simple columnar epithelium of mucous type with basely located nuclei and eosinophilic cytoplasm contains granular secretion. The gross and microscopic examination of the four accessory sex glands gave valuable information in the future pathology diagnosis of the accessory sex glands of the Barki bucks.

Intraosseous angiolipoma of the mandible: case report of a rare entity

<h3>Background</h3> Intraosseous angiolipoma is a benign adipocytic lesion that may occur at any bony site, with predilec- tion for the long bones, ribs, vertebrae, and the head and neck region. As the name suggests, the lesion is character- ized by the presence of mature adipocytes associated with increased vascularity. Intraosseous angiolipoma of the mandible is an exceedingly rare condition first described by Polte in 1976. Until the present date, only four cases were documented in the English literature. Case description: We report a case of a 58 year-old woman presenting with an asymptomatic lesion of the mandible. CBCT examination shows a hypodense area extending from the api- cal region of the mandibular first molar to the mid portion of the mandibular ramus, right side. Lesion is localized, multilocular, with scalloped borders and partially corticated. There is thinning of the facial and lingual cortices, with focal areas of expansion and bone discontinuity. The cortices of the inferior alveolar canal are not visual- ized in some portions. No root resorption is identified. The clinical impression was ameloblastoma. Enucleation and curettage was performed. Microscopic examination reveals the presence of mature adipocytes surrounded by fibrous connective tissue septa featuring numerous vascular channels. Scattered chronic inflammatory cells includ- ing mast cells are seen. No cellular atypia or increased mitotic activity are identified. <h3>Conclusion</h3> We present the fifth case of an intraosseous angiolipoma involving the mandible. The lesion has been classified as either a hyper- plastic process or a benign neoplasm. The differential diagnosis often includes vascular lesions, benign odontogenic entities, central giant cell granuloma, or low-grade intraosseous malignancies. Due to the scarcity of case reports, the nature of the lesion as well as its preferred treatment method are still to be determined.

The Anatomy, Physiology, and Histology of Optic Nerve

The optic nerve (Nervus opticus) is the second of the 12 cranial nerves and is considered to be a part of the central nervous system. It carries visual information from the neurosensory retina to the occipital cortex. The nerve consists of the axons of retinal ganglion cells (RGCs). These axons extend in an organized pattern from the RGC soma to the lateral geniculate nucleus where the neuron's synapse. The nerve is subdivided into fascicles by connective tissue and glial septa, and it is surrounded by cerebrospinal fluid. The optic nerve has intraocular, intraorbital, intracanalicular, and intracranial parts and a length of approximately 50 mm. The central retinal artery, the Zinn-Haller circle formed by the short posterior ciliary artery extensions, and pial vessels take part in the blood supply of the nerve.

Metastatic Canine Phaeochromocytoma with Unusual Manifestation

We report the clinical, pathological and immunohistochemical characteristics of a phaeochromocytoma (PCC) in a 9-year-old male neutered Golden Retriever dog. The dog presented with acute onset of deteriorating cervical pain but was otherwise normal on general physical and neurological examinations. Magnetic resonance imaging of the cervical spine revealed a focal, extramedullary, infiltrative, poorly demarcated, heterogeneous mass with moderate contrast enhancement at the left cranial articular process of C2, associated with osteolysis and pathological fractures of C2, and marked soft tissue trauma. Due to the severe lesions and grave prognosis, the dog was euthanized. Post-mortem examination revealed severe enlargement of the right adrenal gland due to a neoplasm of the adrenal medulla. C2 was lytic and there was a white, well-demarcated, firm neoplastic mass in the surrounding musculature. Neoplasms were also present in, and adjacent to, the prostate gland and in pulmonary lymph nodes. Histologically, the neoplasms were composed of dense sheets and nests of small, round to polyhedral cells with frequent palisading along fine connective tissue septa, karyomegaly, multinucleated cells and frequent mitotic figures. Immunohistochemically, neoplastic cells expressed chromogranin A and synaptophysin. Gross, microscopic and immunohistochemical findings support the diagnosis of PCC, originating from the right adrenal gland, with multiple metastases in the cervical spine, prostate gland and pulmonary lymph nodes. This case highlights the difficulty of intra-vitam diagnosis of PCC as its manifestation can be highly variable. PCC should be considered as a rare but possible differential diagnosis for painful vertebral masses in elderly dogs.