Conjunctival Fungal Flora Research Articles

Conjunctival Fungal Flora in a Tertiary Eye Hospital in Nigeria.

The aim of this study is to determine the hospital incidence and pattern of conjunctival fungal flora in adult patients at the Guinness Eye Center Onitsha, Nigeria. New adult patients, without anterior segment disease, were randomly recruited. Using a sterile swab stick, specimen was taken from the inferior conjunctival fornix of each participant's right eye and inoculated into Sabouraud dextrose agar slant in a test tube and incubated at 27°C. The specimens were examined for fungal growth every 48 h for 4 weeks. Specimens with fungal growth were further examined under a high power microscope for fungal identification and characterization. A total of 225 patients (105 males, 120 females) were examined. The age range was 18-75 years; mean age was 41 ± 17.1 years; 62 (27.6%) were culture-positive: 25 (40.3%) were males and 37 (59.7%) were females (P >0.05). Both moulds and yeasts were isolated with moulds constituting 44 (74.2%). Aspergillus [26 (41.9%)] and Candida [16 (25.8%)] were the commonest organisms. Participants >60 years had the greatest burden. Pensioners (61.5%), traders (44.0%), farmers (30.1%), and artisans (27.3%) were occupational groups with significantly higher proportions of culture-positive specimens (P < 0.05). Over a quarter of new adult patients without anterior segment disease harbour fungi, some of which are pathogenic, in their conjunctival fungal organisms. While Aspergillus and Candida were the commonest isolates, older participants, pensioners, traders, farmers, and artisans had significantly higher proportion of culture-positive specimens. These findings should be considered when formulating pre-operative guidelines for ocular surgery in our environment.

Conjunctival bacterial and fungal flora in clinically normal sheep

ObjectivesThe aim was to identify conjunctival bacterial and fungal flora in clinically normal sheep.DesignProspective study.SettingTuscany.Participants100 eyes from 50 adult Massese female sheep were examined. The sheep included in the study...

Ocular fungal flora from healthy horses in Iran

This study was carried out in order to isolate and identify the normal conjunctival fungal flora from Caspian miniature, Thoroughbred, Turkmen and Persian Arab breeds in Tehran, Iran. A total of seventy-two adult healthy horses were studied. Ocular samples were collected from right and left eyes by using sterile cotton swabs; samples were cultured on Sabouraud dextrose agar and incubated at 30°C for 7-10 days. Molds and yeasts were identified using macro and micro-morphological and physiological characteristics. Number of fungal colonies per eye varied between 0 and 123 colony forming units (CFUs). The most predominant fungal isolates were Aspergillus (19.9%), Rhizopus (15.9%) and Penicillium (15.1%). No significant differences were observed between types of eye fungal floras in different breeds. Caspian miniature horses had significantly the highest number of fungal isolates in compare with other breeds (P<0.001), however no significant difference was observed among other breeds under study. The fungal isolates were almost the same as with studies performed in other countries, although differences in species isolated could be related to geographic and climate difference.

Conjunctival and cutaneous fungal flora in clinically normal dogs in Southern France

The objectives of the present study were to describe the fungal flora of the conjunctiva in clinically normal dogs in southern France and to compare the fungal flora from the conjunctiva and that from adjacent skin areas (medial canthus and nose). Fifty dogs aged from 5 months to 13 years were investigated for conjunctival fungal flora. Specimens were obtained from both conjunctivae and the skin of the medial canthus and from the surface of the nose using sterile cotton-wool swabs and a piece of sterile carpet, respectively. Identification of fungal colonies was achieved recording the genus level on the basis of macro- and microscopic features. Fourteen fungal genera (most frequently Alternaria, Cladosporium, Penicillium and Aspergillus) were isolated from the conjunctival fornix of 7 dogs (14%). There was no significant effect of sex or age on frequency of fungal isolation. Eleven dogs (22%) had a positive mycological culture from at least one eye. Fungi found in the conjunctival fornix of dogs might represent transient seeding from the environment, as suspected in other animal species. The presence of conjunctival fungal organisms was correlated to the presence of fungi on the skin.

Evaluation of the conjunctival fungal flora and its susceptibility to antifungal agents in healthy horses in Switzerland

To characterize the conjunctival fungal flora and to determine the susceptibility of 2 isolated molds to antifungal drugs in samples of 64 healthy horses from The National Stud in Switzerland. Conjunctival cytobrush samples were collected from both eyes of 64 ophthalmologically normal horses in August 2012 and subsequently cultured on Sabouraud's agar medium. Growing fungi were identified and counted. Etests or broth microdilution tests for Aspergillus fumigatus and Eurotium amstelodami were carried out to determine antifungal drug sensitivity. These species had previously been detected in samples from eyes with keratomycosis in Switzerland. Minimal inhibitory concentrations (MICs) for voriconazole, fluconazole, itraconazole, amphotericin B, and miconazole were recorded. Fifty-nine of the horses were tested positive for fungal growth from at least one eye (92%). Eleven genera of fungi were identified. The most common fungal genera were Alternaria, Eurotium, Rhizopus, and Cladosporium. Aspergillus spp. and Penicillium spp. were isolated frequently, while no Fusarium spp. was found. In only 2 cases, yeasts were identified as Candida guilliermondii. For certain fungal species, the type of bedding and housing appeared to influence their prevalence. Susceptibility testing of A. fumigatus showed lowest MICs for voriconazole, E. amstelodami for voriconazole and itraconazole. High MICs for fluconazole were detected for all tested fungi while MICs for amphotericin B and miconazole were variable. A large range of fungal mold species was identified including A. fumigatus and E. amstelodami, which have been causative agents of keratomycosis in Switzerland. Best in vitro susceptibility results for these two species were obtained for voriconazole.

Seasonal prevalence of fungi in the conjunctival fornix of healthy cows during a 2-year study

The aims of the present paper were to: (i) identify and quantify conjunctival fungi isolated from healthy cows; (ii) verify the influence of different methods of farm management on the prevalence (percentage of positive cultures for each fungal species per farm) of conjunctival fungi. Forty Friesian and twenty Limousin female cows aged 1-10 years stabled in three farms with different managements (farm 1: cows housed strictly indoors; farm 2: cows housed outside during the day and inside the stall during the night; farm 3: cows housed strictly outdoors) were investigated for conjunctival fungal flora. Air and food were also tested. Specimens were collected every season during a 2-year study. Identification of colonies of filamentous fungi was achieved to the genus level on the basis of macro- and microscopic features. The total number of eyes positive for fungi ranged from 85 to 100% at farm 1, from 65 to 95% at farm 2, and from 55 to 95% at farm 3. Fungi most frequently isolated from conjunctival fornix were Cladosporium spp. and Penicillium spp. Statistical analysis did not show any differences in fungal prevalences among the three farms during the same season. Some fungal species were consistently isolated while others were intermittently isolated. Fungi found in the conjunctival fornix of cows might represent transient seeding from the environment, as suspected in other species. The prevalence of conjunctival fungal organisms is not different in cattle housed indoors vs. outdoors.

Conjunctival fungal flora in healthy donkeys

To identify and quantify ocular fungi from healthy donkeys living in the center of Italy. One hundred and two Amiata donkeys were examined. Conjunctival swabs from both eyes were seeded onto Sabouraud dextrose agar (SDA) and malt extract agar (MEA), and incubated at 25 degrees C over a 10-day period. Filamentous fungi identification was achieved to the genus level; yeast colonies were identified for macro-micromorphologic and physiological characteristics. Eighty-one donkeys out of 102 (79.4%) were positive for fungi; 47/102 (46.1%) had positive cultures from both eyes. Most frequently recovered fungal genera were Aspergillus spp., Penicillium spp., Cladosporium spp., Acremonium spp. Different fungal genera and/or species were recovered from the same donkey in 43 cases (42.1%). Yeasts were isolated from five subjects; the yeasts were never associated with molds. The number of colony forming units (CFU) ranged from 1 to 100. Aspergillus was the most commonly isolated fungal genus (33%). This result agrees with the findings of similar surveys carried out in horses. There was a remarkable presence of fungi and perfect forms. These observations may be explained by the optimal conditions for presence and development of fungi in the conjunctival fornix microenvironment in Amiata donkeys.

Estudo da microbiota fúngica da conjuntiva ocular em portadores de hanseníase e seus comunicantes

To study the conjunctival fungal flora of patients with leprosy who live in the colony hospital and of those who deal with "Hansen" patients. Sixty-one eyes (61 patients) with Hansen's disease and twenty-five healthy eyes (25 individuals) of individuals who deal with patients were investigated. Conjunctival fungi growth occurred in 19.67% and 20% of the patients and healthy individuals, respectively. There was no significant difference (p<0.05) regarding fungi percentage of fungi isolated from the conjunctiva between both groups. Candida ssp was the most frequent fungus isolated from the conjunctiva of patients with Hansen's disease, whereas Penicillium spp was the most frequent fungus isolated from the group of people who deal with these patients. The most frequent yeast isolated from conjunctival samples of leprosy patients was Candida, which could be explained by cellular immunodeficiency of these patients, associated with dry eye and the prolonged use of antibiotics.

Fungal flora of normal eyes of healthy horses from the State of Rio de Janeiro, Brazil.

The conjunctival fungal flora of 32 adult horses with normal eyes (n = 64) from the State of Rio de Janeiro in Brazil was identified in the fall of 2000 using horses of different breeds, both genders and aged 5-19 years old. The culture samples were taken from the conjunctival sac of both eyes with a sterile cotton swab wetted with saline solution, seeded in Sabouraud's dextrose agar with chloramphenicol, and incubated for 5 days at an average temperature of 25 degrees C. The number of fungal colonies per eye varied between 0 and 250 colony forming units (CFUs). There were often differences in colony types between eyes of the same animal. Filamentous fungi of genera were isolated and identified in the following proportion of the total genera of fungal colonies isolated: Aspergillus (32.2%), Penicillium (25.8%), Scopulariopsis (15.9%), Trichoderma (11.2%), Cladosporium (5.6%), Mucor (2.1%), Syncephalastrum (2.1%), Eurotium (1.7%), Geotrichum (0.9%), Rhizopus (0.9%), Gliomastix (0.4%), Fusarium (0.4%), Staphylotrichum (0.4%) and Verticillium (0.4%). Yeast genera represented 9% of the total isolates. Over half the horses had at least one normal eye with either Aspergillus, Penicillium, Trichoderma or Scopulariopsis isolated, which is a departure from other studies of the normal horse eye.