SummaryThe use of grafted tomato plants is expanding. An in vitro propagation system was established for three commercial F1 cultivars (‘Garnet 622’, ‘Jumbo’ and ‘Marvel’) to combine micropropagation with micrografting. Nodal tissue explants were excised from the central stem of plantlets and micropropagated on MS medium. A micropropagation rate of 6 shoots per explant was obtained for all three hybrids. Micropropagated shoots were then micrografted onto 3 week-old ‘RT-79’ rootstocks with a union rate of over 80%. After acclimatisation, micropropagated plants were established ex vivo, flowered and matured normally. In addition, regeneration protocols were tested using cotyledon explants from 10 d-old seedlings. Adventitious shoot organogenesis from three parts of the cotyledon explants (proximal, middle, distal) was monitored on MS media supplemented with different concentrations of zeatin (Z) and indole-3-acetic acid (IAA). The highest percentage of shoot formation was observed on MS medium supplemented with 0.5 mg l–1 Z and 0.1 mg l–1 IAA acid with cotyledon explants that originated from the proximal part. Up to 80% of explants formed adventitious shoots, with an average of three shoots per explant. Three week-old regenerated shoots were placed on MS medium without growth regulators for elongation, rooting and further development of plantlets.