Non-esterified Fatty Acid Concentrations Research Articles

Supplementation of feed and water after long-duration road transportation: The effects on welfare and rumen fermentation in goats.

Water and feed are needed for livestock during their long-duration road transportation. However, limited information is available on the need to supply water and feed to livestock at temporary holding stations after road transportation. This study aimed to evaluate the effect of providing water and feed at holding stations on the welfare of goats in mimic surroundings. A total of 24 Guizhou black goats were randomly divided into three groups of eight goats each as follows: deprived of water and feed (TRT0), supplemented with water ad libitum (TRT1), and supplemented with water and feed ad libitum (TRT2). Blood and rumen fluid samples were collected before loading (denoted as "PRE" in this article) and after transport (denoted as "POST" in this article). Statistical analysis was performed via the SAS procedure PROC MIXED. The 10-h road transportation period reduced body weight in TRT0 goats (p < 0.05) but not in TRT1 and TRT2 (p > 0.05). TRT0 and TRT1 goats had POST plasma glucose concentrations above their PRE values (p < 0.05). The PRE-plasma urea nitrogen (PUN) levels were higher in TRT2 compared to TRT0 (p < 0.05) goats, while the POST-PUN levels increased in TRT1 compared to TRT0 goats. The POST non-esterified fatty acid (NEFA) concentration was higher for TRT0 compared to that in TRT1 and TRT2 (p < 0.05) goats. No difference was observed for plasma profiles of malondialdehyde (MDA) and superoxide dismutase (SOD) (p > 0.05). TRT2 goats had higher POST glutathione peroxidase (GSH-Px) activity than TRT0 and TRT1 (p < 0.05) goats. TRT0 goats had higher POST plasma glucagon (GC) compared to TRT2 (p < 0.05) and had increased values compared to their own PRE level as a result of road transportation (p < 0.05). TRT2 goats resulted in a lower POST plasma heat-stressed protein-70 (HSP-70) level than TRT0. There was no difference in ruminal pH (p > 0.05). Ruminal total VFA (acetate, propionate, butyrate), and the NH3-ammonia profiles showed a decrease (p < 0.05) after transportation in all groups. Ruminal microcrystalline cellulose, xylanase, cellobiase, and carboxymethyl cellulose activities were unaffected (p > 0.05). These combined results imply that water and feed supplementation to livestock can effectively alleviate stress responses in goats subjected to road transportation and emphasize the necessity to establish water and feed supplies even at a temporary holding pen.

Eucalcemia during lipopolysaccharide challenge in postpartum dairy cows: I. Clinical, inflammatory, and metabolic response

Hypocalcemia induced by immune activation is a conserved response across mammalian species; however, administration of Ca is discouraged in other species as it is associated with increased morbidity and mortality. Early postpartum cows experience a decrease in circulating Ca concentration following acute inflammation. Corrective Ca therapy during the transition period, particularly in dairy cows experiencing acute disease, is common practice. However, the effect of Ca administration on the inflammatory response during acute immune activation is unknown. Our objective was to compare the clinical, inflammatory, and metabolic response to an intravenous (IV) lipopolysaccharide (LPS) challenge between postpartum cows infused, or not, with IV Ca to maintain eucalcemia. Cows (n = 14, 8 ± 1 d in milk) were enrolled in a matched-pair randomized controlled design to receive IV Ca (IVCa) or sterile 0.9% NaCl (CTRL) during an IV LPS challenge (0.040 or 0.045 µg of LPS/kg of body weight over 1 h). Ionized Ca (iCa) was monitored cow-side, and IV Ca infusion was adjusted in a eucalcemic clamp for 12 h following the start of LPS infusion. Cows were monitored during the 24 h following challenge and serial blood samples were collected to quantify concentrations of glucose, β-hydroxybutyrate, nonesterified fatty acids, urea nitrogen, cytokines, acute-phase proteins, and cortisol. Blood iCa concentration decreased to 0.87 ± 0.03 mM in CTRL during challenge, and by design, iCa concentration was maintained within 3% of baseline in IVCa. Body temperature, heart rate, and respiratory rate were monitored for 24 h following the start of challenge and did not differ between groups. A treatment × time interaction was identified such that serum cortisol concentrations increased in both groups at 2 h but decreased to a greater extent at 6 h in IVCa compared with CTRL. Rumination time (min/h) over the first 12 h following challenge was greater in IVCa, but total rumination time in the 24 h following challenge did not differ from CTRL. Serum glucose and nonesterified fatty acid concentrations decreased, and β-hydroxybutyrate and urea nitrogen concentrations increased over time, but did not differ between groups. Acute leukopenia occurred in both groups at 4 h before leukocytosis was observed at 24 h with total white blood cell counts returning to baseline within 72 h. Plasma concentrations of tumor necrosis factor (TNF) and interleukin-10 (IL-10) increased within 1 h following the start of challenge and did not differ between groups. Serum haptoglobin and serum amyloid A concentrations increased within the 24 h following challenge and were elevated through 72 h but did not differ between groups. Eucalcemia during the acute systemic inflammatory response did not alter the TNF or IL-10 cytokine response, or the acute-phase protein SAA and haptoglobin response in this LPS challenge model; however, eucalcemia was associated with a more rapid decline in cortisol response and greater rumination time in the first 12 h following challenge. We did not find evidence that eucalcemia exacerbated the inflammatory response in early postpartum cows, but Ca administration may alter the clinical response to acute systemic inflammation.

Metabolic profile of transition period in ewes and its influence on passive immunity transference in lambs.

The intensification of ewe production, focusing on increasing prolificity, results in a higher incidence of metabolic disorders, especially in the transition period, which can lead to production losses with consequences for the health of the animals. The objective of this study was to evaluate the metabolic profile in transition period Lacaune ewes according to the parity and litter size born and its influence on the transference of passive immunity. Nineteen Lacaune ewes, with initial mean body weight of 76.36 ± 10.37kg (SD), kept in a semi-extensive system and receiving the same diet, were used. Ewes were classified according to parity (primiparous and multiparous) and the litter size born at parturition (1, 2, and 3). On days - 14, - 7, and - 4 of the expected date of parturition and on days 1, 4, 7, and 14days postpartum, blood sampling, the evaluation of the body condition score (BCS), and the weighing of animals were performed. Blood samples were taken from the lambs 48h after birth. Colostrum was collected up to 6h postpartum to determine the brix percentage. Serum concentrations of total plasma proteins (TPP), albumin, aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), paraoxonase (PON1), calcium (Ca), and magnesium (Mg) were evaluated. In lambs, the blood parameters evaluated were TPP, albumin, GGT, and PON1. Lamb plasma was used to estimate colostrum efficiency, brix percentage immunity, and total plasma protein (PPT). Productive data, pre- and postpartum metabolic variables for ewes, lambs, and those related to colostrum brix were analyzed. The BCS had a reduction (p < 0.0001) in the postpartum period, which is evidenced on days 7 and 14 (2.88 and 2.73, respectively). Serum concentration of BHB presented the highest concentrations in the prepartum period for ewes that gave birth to triplets, from day - 14, and for ewes that gave birth to twins on day - 4 (p = 0.0245). Serum concentration of NEFA demonstrated the highest value on day 14 in primiparous (1.07; p = 0.0402). Calcium had the lowest concentrations on day 1 (8.85mg/dl, p < 0.0001) and magnesium on day 3 (1.79mg/dl, p < 0.0001) postpartum. The multiparous with two lambs had higher brix percentages (30.5%), and the lowest value was observed in primiparous ewes that gave birth to a single lamb (27.06%; p = 0.0395). Triplet lambs had the lowest weight (3.73kg, p = 0.0007), and the best brix percentage in plasma was observed in twins (10.29%, p = 0.0174). Regardless of the parity, the ewes that presented the greatest metabolic challenge were those that gave birth to triplets, and these lambs presented the worst immunity and the lowest live weight. The quality of colostrum was influenced by the parity, and multiparous ewes that gave birth to triplets had the lowest brix percentage in the colostrum.

Abstract P308: The Determinants of Fasting and Post-Load Non-Esterified Fatty Acids in Older Adults: The Cardiovascular Health Study

Aim: Non-esterified fatty acids (NEFA) are associated with key cardiometabolic diseases of aging and are potential targets for prevention, but their population-level correlates remain uncertain. We sought to identify modifiable factors associated with higher fasting and post-load NEFA levels in older adults. Methods: We used linear multivariable regression models to determine the cross-sectional associations of demographic, anthropometric, lifestyle characteristics, and medication use with serum fasting and post-load NEFA concentrations amongst community-dwelling older adults enrolled in the Cardiovascular Health Study (n = 1,924). Results: In general, fasting NEFA levels demonstrated a broader set of determinants, while post-load NEFA were more consistently associated with metabolic factors. Waist circumference and weight were associated with higher fasting and post-load NEFA. Cigarette smoking and caffeine intake were associated with lower levels of both species, and moderate alcohol intake was associated with higher fasting levels whereas greater consumption was associated with lower post-load levels. Unique factors associated with higher fasting NEFA included female sex, higher age, loop and thiazide diuretic use and calcium intake, while factors associated with lower fasting levels included higher educational attainment, beta-blocker use, and protein intake. Hours spent sleeping during the daytime were associated with higher post-load NEFA, while DASH score were associated with lower levels. Conclusion: Fasting and post-load NEFA appear to have both common and unique modifiable risk factors, including sociodemographics, anthropometric, medications, and diet. Post-load NEFA were particularly sensitive to metabolic factors, while a broader range of determinants were associated with fasting levels. These factors warrant further study as targets for lowering levels of NEFA in older adults..

Association between Tryptophan Metabolism and Inflammatory Biomarkers in Dairy Cows with Ketosis

Dairy cows with ketosis have high circulating beta-hydroxybutyric acid (BHBA) concentrations alongside which inflammation is concomitantly developed. Tryptophan (Trp) is an essential amino acid that participates in the regulation of the inflammatory response. However, the association between Trp metabolism and inflammation in dairy cows with ketosis remains unclear. Therefore, blood samples from healthy (n = 10) and ketotic (n = 10) primiparous dairy cows were collected at the calving date and the day of ketosis diagnosis (7 days in milk (7 DIM)). Serum levels of non-esterified fatty acids (NEFA), BHBA, haptoglobin (HP), serum amyloid A (SAA), lipopolysaccharide, and cortisol were analyzed. Tryptophan and its metabolites were quantified using liquid chromatography-tandem mass spectrometry. At 7 DIM, the concentrations of NEFA, BHBA, HP, and SAA were higher and the levels of Trp, kynurenine (KYN), indoleacetic acid, indole-3-lactic acid, and 3-indoxyl sulfate were lower in the dairy cows with ketosis compared with those in the healthy cows. However, the KYN/Trp and melatonin/Trp ratios increased in the cows with ketosis. At the calving date, the serum lipopolysaccharide levels did not differ between the healthy and ketotic cows, whereas the levels of NEFA, HP, and cortisol increased in the ketotic cows. Correlation analysis showed that Trp deficiency and elevated Trp metabolism in the dairy cows occurred during ketosis. Overall, our results suggest that abnormal Trp metabolism may contribute to the pathogenesis of ketosis.

Association of maternal late-gestation lipid mobilization and their offspring's disease risk during the pre-weaned period and performance through first lactation: A cohort study in a dairy herd.

Excessive maternal lipid mobilization in late gestation may impact the immune function of the newborn. However, the long-term effects remain unknown. The objective was to explore associations between excessive maternal lipid mobilization in the last 2 weeks of gestation with offspring health and performance. A retrospective study was performed including 1,511 calves (heifer = 692, bull = 819) born between 2015 and 2020 in one MI farm. Plasma non-esterified fatty acids (NEFA) was measured from cows 7 to 14 d before calving. Calves were categorized in 2 groups based NEFA concentration: physiological lipid mobilization (PLM = 1,373; NEFA <0.3 mM) and excessive lipid mobilization (ELM = 138; NEFA ≥0.3 mM). Calf records were obtained from the herd's management software. Outcomes of interest were the hazard of pre-weaned digestive and respiratory disease, pre-weaned ADG, age at first breeding and calving, first lactation 305 d mature equivalent milk yield (305ME), and survival until first calving. Statistical models included dam NEFA category adjusted by year and season of birth, parity of the dam, and sex of the calf. Cox proportional analysis was used to determine the hazard of a pre-weaned health event, first breeding, and first calving. Linear regression was used to evaluate ADG and 305ME. The survival until first calving was analyzed with logistic regression. No difference was detected in the hazard of diarrhea (HRPLM vs. ELM = 1.06; 95% CI = 0.82-1.38) and respiratory disease (HRPLM vs. ELM = 1.29; 95% CI = 0.79-2.10) by NEFA category in the pre-weaned period. Also, no difference was detected for the LSM (±SE) of pre-weaned ADG (PLM = 0.77±1.55, ELM = 0.72±2.76 kg/d). In heifers, the hazard for first breeding favored the PLM group (HRPLM vs. ELM = 1.59; 95% CI = 1.18-2.12), with a reduced median age at first breeding (PLM = 400 d, 95% CI = 397-402; ELM = 412 d, 95% CI = 404-421). However, NEFA category was not associated with the hazard of first calving (HRPLM vs. ELM = 0.94; 95% CI = 0.69-1.27), first lactation 305ME (PLM = 16,665±165 kg; ELM = 16,256±532), the odds of presenting at least 1 health event in the first lactation (ORPLM vs. ELM = 0.78; 95% CI = 0.41-1.49), or the odds of leaving the herd before first calving (ORPLM vs. ELM = 1.21; 95% CI = 0.56-2.02). Overall, dam ELM affected the hazard of first breeding but no other indicators of health or long-term performance. However, associations between maternal lipid mobilization and calf outcomes cannot be excluded, as the NEFA cut-off used has not been established as a predictor of offspring health and performance.

Influence of lactation stage on heat production and macronutrient oxidation in dairy cows during a 24-hour fasting period

Understanding nutrient utilization and partitioning is essential for advancing the efficiency of dairy cattle. Our objective was to determine if dairy cows exposed to a 24-h fasting period differ in heat production (HP) and macronutrient oxidation at different stages of lactation. Twelve primiparous, lactating German Holstein dairy cows were used in a longitudinal study design spanning from 2013 to 2014. Dairy cows were housed in respiration chambers during 3 stages of the lactation cycle: early (mean ± SD; 28.8 ± 6.42 d), mid- (89.4 ± 4.52 d), and late (293 ± 7.76 d) lactation. Individual CO2, O2, and CH4 gas exchanges were measured every 6 min for two 24-h periods, an ad libitum period and fasting period (RES). Blood was sampled at the start and end of the RES period. Gas measurements were used to calculate HP, net carbohydrate oxidation (COX), and net fat oxidation (FOX). Measurements were corrected with metabolic BW (kg of BW0.75; cBW). The RES period for each stage of lactation was further subdivided into the start (RESstart) and end (RESend) by averaging the first and last 2 h of the RES period. The net change was calculated as RESend - RESstart. All energy variables differed among lactation stage within the RES period except for HP/cBW. As expected, COX, COX/cBW, COX/HP, HP, and HP/cBW, were greater at the RESstart compared with RESend, whereas FOX, FOX/cBW, and FOX/HP were greater at the RESend except for FOX and FOX/cBW during mid lactation, which was only a tendency for a difference. The net change for COX, COX/cBW, HP, HP/cBW, and FOX/cBW did not differ among stages of lactation. Despite detecting a tendency for a difference among stage of lactation for FOX, pairwise analysis revealed no differences. Plasma triglyceride, urea, and nonesterified fatty acid concentrations were greater at RESend than RESstart. The net change for plasma glucose, urea, β-hydroxybutyrate, and nonesterified fatty acid concentrations were greater in early than late lactation. Our results demonstrate that despite differences in absolute measurements of energy variables and plasma metabolites, the change in whole-body macronutrient oxidation and HP as cows' transition from a fed-like state to a starvation-like state during a 24-h fasting period is consistent throughout lactation.

Liver fibrosis is a common pathological change in the liver of dairy cows with fatty liver

Fatty liver (i.e., hepatic lipidosis) is a prevalent metabolic disorder in dairy cows during the transition period, characterized by excess hepatic accumulation of triglyceride (TG), tissue dysfunction, and cell death. Detailed pathological changes, particularly hepatic fibrosis, during fatty liver remain to be determined. Liver fibrosis occurs as a consequence of liver damage, resulting from the excessive accumulation of extracellular matrix, which distorts the architecture of the normal liver, compromising its normal synthetic and metabolic functions. Thus, we aimed to investigate liver fibrosis status and its potential causal factors including oxidative stress, hepatocyte apoptosis, and production of inflammatory cytokines in the liver of cows with fatty liver. Forty-five dairy cows (parity, 3-5) were selected, and liver biopsy and blood were collected on the second week postpartum (days in milk, 10-14 d). On the basis of the degree of lipid accumulation in liver, selected cows were categorized into normal (n = 25; TG <1% wet wt), mild fatty liver (n = 15; 1% ≤ TG <5% wet wt), and moderate fatty liver (n = 5; 5% ≤ TG <10% wet wt). Compared with normal cows, blood concentrations of nonesterified fatty acids and β-hydroxybutyrate, along with alanine aminotransferase and aspartate aminotransferase activities, were greater in the cows with fatty liver (mild and moderate). Hepatic extracellular matrix deposition, as indicated by Picrosirius red staining, was greater in cows with fatty liver than those with normal ones. In addition, we observed an increased proportion of collagen type I fiber in extracellular matrix with increased lipid accumulation in the liver. Compared with normal cows, the area of α-smooth muscle actin (α-SMA)-positive staining along with the mRNA abundance of collagen type I α 1 (COL1A1), ACTA2 (gene encoding α-SMA), and transforming growth factor-β (TGFB) were greater in cows with fatty liver. Compared with normal cows, hepatic contents of malondialdehyde, glutathione disulfide, and 8-isoprostane were greater, whereas total antioxidant capacity, the hepatic content of glutathione, and activities of antioxidant indicators, including superoxide dismutase, glutathione peroxidase, and catalase, were lower in cows with fatty liver. The number of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling-positive cells and abundance of apoptosis-related molecules BAX, CASP3, CASP8, and CASP9 were greater in cows with fatty liver. However, mRNA abundance of the anti-apoptotic gene BCL2 did not differ. The mRNA abundance of pro-inflammatory cytokines including tumor necrosis factor-α (TNFA), interleukin-1β (IL1B), and interleukin-6 (IL6) was greater in the liver of cows with fatty liver. Overall, the present study indicated that fibrosis is a common pathological response to liver damage and is associated with oxidative stress, hepatocyte death, and inflammation.

Pregnancy but Not Moderate Undernutrition Attenuates Insulin Suppression of Fat Mobilization in Sheep , ,

Nonpregnant and late-pregnant ditocous ewes were fed either to maintain zero energy balance in maternal tissues (fed) or at 50% of this level (underfed) for several weeks. Plasma concentrations of nonesterified fatty acids (NEFA) and glycerol were measured under basal conditions and during infusion of various doses of insulin while maintaining euglycemia (hyperinsulinemic, euglycemic clamp technique). Pregnancy and undernutrition separately increased basal plasma NEFA concentration in an additive manner; plasma glycerol was increased by pregnancy but unaffected by undernutrition. The molar ratio of NEFA to glycerol was significantly greater in underfed ewes. Analysis of dose-response relations between plasma insulin and metabolites during insulin infusions showed that maximally insulin-suppressed concentrations of NEFA and glycerol were significantly greater in pregnant than in nonpregnant ewes but were unaffected by undernutrition. Neither pregnancy nor undernutrition affected the maximally insulin-suppressed NEFA to glycerol ratio, or the plasma insulin concentration for 50% maximal responses to insulin of plasma NEFA, plasma glycerol, or the plasma NEFA to glycerol ratio. Thus, even in ewes at or close to zero energy balance, pregnancy seems to reduce adipose responsiveness but not sensitivity to the antilipolytic effect of insulin. This is another manifestation of the normal development of insulin resistance in maternal tissues during late pregnancy.

Effects of Nutritional Plane at Breeding on Feed Intake, Body Weight, Condition Score, Mass Indexes, and Chemical Composition, and Reproductive Performance of Hair Sheep.

This study was conducted to assess effects of the nutritional plane before and during breeding on feed intake, body weight (BW) change, reproductive performance, body condition score (BCS), body mass indexes (BMI), and body composition of three breeds of hair sheep. Twenty-five Dorper, 27 Katahdin, and 33 St. Croix ewes were allocated to groups and treatments based on initial BW and age. Supplementation treatments were soybean meal fed at 0.16% BW (LS) and a mixture of 25% soybean meal and 75% ground corn given at 0.8% BW (HS; dry matter basis) for 88 to 102 days including 17 days after breeding. Wheat straw intake (% BW) was greater (p < 0.01) for LS than for HS. Average daily gain and BCS change were similar among breeds, but both were greater for HS vs. LS. Birth rate, litter size, total litter birth weight, gestation length, and number of services were similar among breeds (p > 0.05), although individual lamb birth weight was lower for St. Croix than for Katahdin (p < 0.05). None of these reproductive variables were influenced by treatment or the breed × treatment interaction (p > 0.05). There were no breed differences in whole body concentrations of water, fat, protein, ash, or energy in week 0 or 4, but in 8 week concentrations of water, protein, and ash were greatest among breeds for St. Croix (p < 0.05) and levels of fat and energy were lower for St. Croix than for Dorper (p < 0.05). In week 8, total amounts and concentrations of fat and energy were greater for HS vs. LS and those of water, protein, and ash were less for HS (p < 0.05). There were interactions (p < 0.05) between treatment and period in blood concentrations of cholesterol and non-esterified fatty acids and between breed, treatment, and period in level of triglycerides and total antioxidant capacity. The concentration of cortisol was greater for HS vs. LS (p < 0.05). In conclusion, supplementation did not influence reproductive performance despite differences in BW and BCS change, which may be due to the initial moderate BCS and greater wheat straw intake for the LS treatment.

Effects of a genetic variant rs13266634 in the zinc transporter 8 gene (SLC30A8) on insulin and lipid levels before and after a high-fat mixed macronutrient tolerance test in U.S. adults

BackgroundThe common C-allele of rs13266634 (c.973C>T or p.Arg325Trp) in SLC30A8 (ZNT8) is associated with increased risk of type 2 diabetes. While previous studies have examined the correlation of the variant with insulin and glucose metabolism, the effects of this variant on insulin and lipid responses after a lipid challenge in humans remain elusive. The goal of this study was to determine whether the C-allele had an impact on an individual’s risk to metabolic syndromes in U.S. adults. MethodWe studied the genotypes of rs13266634 in 349 individuals aged between 18 and 65 y with BMI ranging from 18.5 to 45 kg/m2. The subjects were evaluated for insulin, glucose, HbA1c, ghrelin, and lipid profiles before and after a high-fat mixed macronutrient tolerance test (MMTT). ResultsWe found that the effects of variants rs13266634 on glucose and lipid metabolism were sex-dimorphic, greater impact on males than on females. Insulin incremental area under the curve (AUC) after MMTT was significantly decreased in men with the CC genotype (p < 0.05). Men with the CC genotype also had the lowest fasting non-esterified fatty acid (NEFA) concentrations. On the other hand, the TT genotype was associated with a slower triglyceride removal from the circulation in men after MMTT. The reduced triglyceride removal was also observed in subjects with BMI ≥ 30 carrying either the heterozygous or homozygous T-allele. Nevertheless, the SNP had little effect on fasting or postprandial blood glucose and cholesterol concentrations. ConclusionWe conclude that the CC genotype negatively affects insulin response after MMTT while the T-allele may negatively influence lipolysis during fasting and postprandial blood triglyceride removal in men and obese subjects, a novel finding in this study.

Roasted Soybeans and an Estrogenic Growth Promoter Affect Growth Hormone Status and Performance of Beef Steers

This study investigated the effects of a roasted soybean (RSB)-supplemented diet and an estrogen implant (SYN; Synovex-S® ear implant, 20 mg estradiol benzoate plus 200 mg progesterone) in young crossbred beef steers on their performance and plasma growth hormone (GH) response to challenge injections of thyrotropin-releasing hormone (TRH) + GH-releasing hormone (GHRH). Twenty individually fed steers (body weight 255 ± 5 kg) were assigned to the following treatments: 1) no SYN and fed a soybean meal-supplemented diet, 2) no SYN and fed the RSB-supplemented diet, 3) plus SYN and soybean meal, and 4) plus SYN and RSB. Steers were fed 1.13 MJ metabolizable energy/kg metabolic body weight daily of an 18% protein diet. After a 5-wk growth period, all steers were challenged (intravenous injection) over a 3-wk period with three levels of a combination of TRH + GHRH (0.1 + 0.01, 1.0 + 0.1, 2.5 + 0.25 µg/kg body weight, respectively). After an additional 3 wk, steers were reimplanted and a second 5-wk growth period was followed by a single challenge of the 1.0 + 0.1 TRH + GHRH dose level. Plasma nonesterified fatty acid concentration was greater when steers were fed the RSB compared with soybean meal (265 vs. 205 µmol/L; P < 0.01; SEM = 9.5). Body weight gains for treatments 1, 2, 3 and 4 were 1.35, 1.21, 1.47 and 1.38 kg/d, respectively (RSB, P < 0.10; SYN, P < 0.07; SEM = 0.06). Gain/dry matter intake (g/kg) means were 184, 167, 197 and 184 (RSB, P < 0.04; SYN, P < 0.07; SEM = 7.5). Feeding roasted soybeans resulted in depressed area under the GH response curve [907, 555, 827 and 989 µg/(L*min) (SYN × RSB, P < 0.03; SEM = 117)] and depressed peak response (37.2, 26.6, 33.5 and 41.1 µg/L [SYN × RSB, P < 0.05; SEM = 4.5]), an effect alleviated by estrogen for young growing steers (Period 1) but not for heavier steers (Period 2).

Evaluation of the diagnostic accuracy of a point-of-care device to measure concentrations of nonesterified fatty acids in serum and whole blood

The objective of this study was to compare measurements of nonesterified fatty acids (NEFA) between the gold standard diagnostic laboratory method and a handheld NEFA meter (Qucare Pro meter, DFI Co. Ltd.). Three experiments were conducted to study the usability of the meter. In experiment 1 we compared results of the meter obtained from measurements in serum and whole blood with results of the gold standard method. Based on the results of experiment 1 we compared the results measured by the meter in whole blood with results obtained from the gold standard method on a larger scale, as we wanted to omit the step of centrifugation with the cow-side test. In experiment 3 we determined the influence of ambient temperature on measurements. Overall, blood samples of 231 cows were collected between 14 and 20 d in milk. The Spearman correlation coefficients (ρ) were calculated and Bland-Altman plots were created to compare the accuracy of the NEFA meter with the gold standard. In addition, in experiment 2 receiver operating characteristic (ROC) curve analyses were performed, to define thresholds for the NEFA meter to detect cows with a NEFA concentration above 0.3, 0.4, and 0.7 mEq/L. In experiment 1, there was a high correlation between NEFA concentrations in whole blood and serum determined by the NEFA meter and the gold standard (ρ = 0.90 for measurement in whole blood; ρ = 0.93 for measurement in serum). In experiment 2, the measurement in whole blood with the NEFA meter was compared with the gold standard. Despite a lower correlation (ρ = 0.79) the ROC curve analyses revealed a high specificity and a moderate sensitivity for lower cut-points (i.e., 0.3 and 0.4 mEq/L). The NEFA meter underestimated especially high concentration of >0.7 mEq/L. Considering thresholds of 0.3, 0.4, and 0.7 mEq/L measured by the gold standard test, sensitivity and specificity were 59.1% and 96.7%, 79.0% and 95.4%, and 86.4% and 95.6%, respectively, when using 0.3, 0.3, and 0.4 mEq/L as thresholds for the NEFA meter. Accuracy was 74.1%, 88.3%, and 93.8% for the 3 thresholds tested. Experiment 3 showed that measurements should be conducted at approximately 21°C (ρ = 0.73) as correlations were poor at 6.2°C and 15.1°C (ρ = 0.18 and 0.22, respectively).

Study of the activity of lipolysis and gluconeognesis in new-child cows with subclinical and clinical ketosis

The article discusses the results of a study of markers of lipolysis and gluconeogenesis in new-calf cows against the background of subclinical and clinical ketosis. The indicator of beta-hydroxybutyric acid in blood serum was chosen as a marker for the formation of groups based on the presence of ketosis and its form. Cows with an indicator of up to 1.0 mmol/l were considered healthy, the presence of subclinical ketosis was determined at a level of beta-hydroxybutyrate from 1.1 to 1.5 mmol/l, clinical ketosis - over 1.6 mmol/l. Cows were bled 3-7 days after calving and a study was made of the concentration of beta-hydroxybutyric acid, glucose, fructosamine, non-esterified fatty acids, cortisol and ALT activity. The results of the study revealed a significant increase in the concentration of non-esterified fatty acids in cows with moderate ketonemia by 46.9%, and in cows with a pronounced increase in the content of beta-hydrosibutyrate in the blood - by 79.8%. When considering the concentration of glucose in cows with different levels of beta-hydroxybutyrate in the blood, there is a slight increase in the indicator by 14.5% (P&gt; 0.05) in cows with subclinical ketosis, but in animals with severe ketonemia, a significant decrease in glucose by 38.3 %. Cows in subclinical and clinical ketosis had significantly higher levels of cortisol (1.9 and 2.4 times, respectively) than cows with low blood levels of betahydroxybutyric acid. At the same time, cows with clinical ketosis showed a significant increase in the concentration of fructosamine by 22.3%. Subclinical ketosis, manifested by a moderate increase in the concentration of beta-hydroxybutyric acid in the blood (1.1 - 1.5 mmol / l), causes adaptive reactions in the body aimed at maintaining glucose homeostasis and extracting lipids from the depot that compensate for a temporary energy deficit. Clinical ketosis is accompanied by dissociation of the regulatory mechanisms of anabolic and catabolic processes in the body of a freshly calved cow.

Association between biochemical parameters and ultrasonographic measurement for the assessment of hepatic lipidosis in dairy cows

Hepatic lipidosis (HL) is a serious metabolic disorder that affects transient high-producing dairy cows. However, there are many laboratory methods could help in the assessment of HL in dairy cows, the definite diagnosis of the disease under field conditions remains a challenge because of the non-specificity of clinical parameters. The aim of the study was to investigate the utility of serum biomarkers, and ultrasonography (US) measurement to diagnose HL in dairy cows. Forty Holstein dairy cows underwent physical examination and were divided into three groups: non-HL, mild HL, and severe HL groups based on US findings, and the concentrations of beta-hydroxybutyric acid (BHBA) and nonesterified fatty acids (NEFAs). Ultrasound images showed hyperechoic lesions and increased pixel intensity (PI) values of liver echotexture in the HL groups. A significant increase was observed in mild and severe HL groups in the activity of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), BHBA, NEFAs, malondialdehyde (MDA), and nitric oxide (NO) while the concentrations of triglyceride (TG), glutathione (GSH), and total antioxidant capacity (TAC) were significantly decreased. The classification accuracy was in the interest of NEFAs (cut off 0.62 mmol/L, sensitivity 82.6%, specificity 91.7%, and an AUC of 0.913) and BHBA (cut off 1.35 mmol/L, sensitivity 91.31%, specificity of 86.4%, and an AUC 0.812) as important diagnostic parameters. This study offers new insights into the importance of serum biochemical parameters and oxidative stress biomarkers especially, AST, NEFAs, BHBA, and GSH with the digital values of US images for prompt use of minimally invasive techniques to diagnose HL under farm conditions. HIGHLIGHTS Serum metabolites and oxidative stress indices could diagnose hepatic lipidosis in dairy cows NEFA and BHBA concentrations and ultrasound digital values can use as a non-invasive way to the classification of hepatic lipidosis degrees in cows under farm condition.

Effect of Dietary Supplemented with Mulberry Leaf Powder on Growth Performance, Serum Metabolites, Antioxidant Property and Intestinal Health of Weaned Piglets

Background: The study aimed to explore the effect of mulberry leaf powder (MP) on the performance, serum metabolites and antioxidant property, as well as intestinal health, of weaned piglets. A total of 120 healthy piglets weighing 8.43 ± 1.91 kg (Duroc × (Landrace × Yorkshire); weaned at 28 d) were chosen and classified into four treatments with three replicates of 10 piglets each based on a randomized complete block design (barrow:gilt = 1:1). The diet treatments were a corn-soybean meal basal diet added with 0% (Ctrl), 2% (MP_2), 4% (MP_4) and 6% MP (MP_6), respectively. The feeding experiment was 28 days in total. The feeding period lasted for 28 days in total. Results: The diet supplemented with 2% MP had no detrimental effects on the growth performance, immunity, enzyme capacity and inflammatory factors, as well as intestinal barrier function. MP_2 is capable of decreasing the levels of serum D-lactic acid and lactate dehydrogenase, enhancing the superoxide dismutase capacity in the liver and diminishing the potential pathogenic bacteria Allisonella in the colon. However, compared with MP_2, MP_6 had unfavorable effects on the average daily gain and average daily feed intake; the concentration of serum non-esterified fatty acids; the activities of superoxide dismutase and glutathione peroxidase and the capacity of lipase and amylase, as well as the intestinal barrier function-related mRNA expression of occludin, claudin-1 and mucin-2 in piglets. Conclusion: Taken together, piglets fed with 2% MP had no adverse effect and was capable of improving the serum metabolites, enhancing the antioxidant capacity (SOD) and lowering the potential pathogenic bacteria of the hindgut (Allisonella). However, the highest concentration of MP (6%) may cause detrimental effects for piglets, which are probably associated with the higher antinutritional factors and fiber. Therefore, the dietary supplementation of 2% MP for piglets may be advisable.

Effects of injectable vitamin E before or after transit on receiving phase growth performance, health, and blood parameters of beef steers.

The objective was to determine the effects of injectable vitamin E (VE) before or after transit on feedlot cattle receiving performance, health, and blood parameters. Angus × Simmental steers (n = 196; body weight [BW] = 163 ± 29 kg) were utilized in a randomized complete block design. Steers were blocked by BW and randomly assigned to 1 of 3 treatments: intramuscular injections of saline pre- and post-transit (CON), intramuscular injections of VE (2,000 mg d-α-tocopherol) pre-transit and saline post-transit (PRE), or intramuscular injections of saline pre-transit and VE (2,000 mg d-α-tocopherol) post-transit (POST). Pre-transit injections were administered on day 0, and steers were transported on day 7 for approximately 4 h (348 km). After arrival, steers were fed a common corn silage-based diet in GrowSafe bunks. Final BW tended to be greater (P = 0.08) for CON steers compared with POST steers while PRE steers were intermediate. From days 7 to 63, treatment affected average daily gain (ADG) with PRE and CON steers exhibiting (P = 0.04) greater ADG compared with POST steers. Dry matter intake (DMI), water intake, and gain to feed from days 7 to 63 were not affected (P ≥ 0.17) by treatment. Day 0 serum α-tocopherol concentrations were considered marginal (2.3 ± 0.2 mg/l). A treatment × day interaction (P < 0.01) was observed for serum α-tocopherol concentrations. Serum α-tocopherol concentrations were greatest for PRE steers on day 7 (prior to and post-transit), but greater for POST steers on dys 10 and 14. Plasma ferric-reducing antioxidant potential concentrations increased (P = 0.04) for POST steers compared with CON steers and PRE steers being intermediate. Plasma non-esterified fatty acids (NEFA) concentrations exhibited a treatment × day interaction (P = 0.04) with CON and POST steers being 16% and 14% greater than PRE steers on day 14, respectively. On day 21, NEFA concentrations were greatest for POST steers compared with PRE steers and CON steers being intermediate. There was no main effect (P ≥ 0.14) of treatment on the number of bovine respiratory disease morbidity treatments. Hair cortisol concentrations were decreased (P < 0.01) 14 days after transit for PRE and POST steers compared with CON steers. Overall, injectable VE administered before or after transit increased serum tocopherol concentrations while reducing stress, but did not improve the growth performance of beef steers during the receiving phase.

Effect of the supplementation with a combination of plant extracts on sow and piglet performance and physiology during lactation and around weaning.

Weaning is a critical period for pigs. Some plant extracts showing antioxidant, anti-inflammatory or antibacterial properties, provided to piglets and/or their dam, may improve piglets' robustness at weaning, thus reducing the need for antobiotics. This study investigated the effects of a maternal and/or a direct supplementation of piglets with a combination of plant extracts on sow and piglet performance and their metabolic, immune, inflammatory, and oxidative status during lactation and around weaning. Sixty-four sows were assigned to the control or treated group. Treated sows were supplemented with a powdered plant extracts supplement daily top-dressed on feed from day of gestation (DG) 106 to day of lactation (DL) 28 and a liquid solution top-dressed on feed on DG109. Within each sow group, litters were divided into two groups: a control piglet group and a treated piglet group. A single dose of a liquid solution was orally given to piglets in the treated piglet group. Piglets were weaned on DL28. Blood samples were collected from sows on DG94, DG112, and DL26 and from 2 piglets per litter on DL3, DL14, DL25, and 5 d postweaning to analyze indicators of metabolic, immune, inflammatory, and oxidative status. Colostrum and milk samples were collected at farrowing, DL6, and 26. Maternal supplementation had no effect on sow metabolic, immune, inflammatory, and oxidative status except for fewer lymphocytes on DG112 (P < 0.05) and a lower plasma concentration of non-esterified fatty acids on DL26 (P < 0.05). Maternal supplementation tended to decrease dry matter and gross energy (P < 0.10) and reduced fat and haptoglobin concentrations (P < 0.01) in milk on DL26. Maternal supplementation had no effect on piglets' growth performance and blood indicators during lactation and around weaning. On DL25, the direct supplementation of piglets decreased their neutrophils proportion (P < 0.05), increased the expression of genes encoding pro- and anti-inflammatory cytokines in whole blood culture in response to lipopolysaccharide (P < 0.05) and tended to decrease the oxidative stress index (P = 0.06). After weaning, these beneficial effects were no longer observed but the supplementation improved piglets' growth performance during the postweaning period (P < 0.05). Plant extract supplementation could thus modify the composition of mammary secretions and improve postweaning performance of piglets potentially related to the modification of their immune and oxidative status before weaning.

Impact of liquid diet supplementation while suckling and weaning age on nutritional status, intestinal health, and immunity of piglets pre- and post-weaning.

New strategies are needed to enhance piglets' robustness and proper functional development and maturation of piglets' intestine before weaning, to reduce the number of antibiotic treatments of diarrheic disorders in newly weaned piglets. It was hypothesized that a liquid nutritional supplement during the suckling period, and/or an increased weaning age, would beneficially impact piglets' gut health and enhance piglets' nutritional status before weaning. Further, it was hypothesized that a high intake of colostrum during the first 24 h after birth would be more advantageous for piglets' growth and robustness when compared to a low colostrum intake (CI). A 2 × 2 factorial design with two nutritional strategies (± supplementation with milk/feed, i.e., milk provided from day 2 shifted to wet feed at day 12 of age) and two weaning ages (days 24 vs. 35) was used. In total, 460 piglets from 24 sows were used for estimation of the individual CI after birth. Provision of the nutritional supplement and the increased weaning age improved the nutritional status of piglets' post-weaning assessed by their blood plasma concentration of albumin (P = 0.04), triglycerides (P = 0.004), and nonesterified fatty acids (P = 0.02). Piglets with high CI demonstrated improved nutritional status when compared to low CI (P = 0.04). Villous height and crypt depth were greater in piglets weaned at day 35 of age in contrast to day 24 of age (P < 0.001) irrespective of the nutritional intervention (P = 0.82). The concentration of branched-chain fatty acids in piglets' digesta was reduced in groups provided the nutritional supplement (P = 0.01), while total short-chain fatty acids were elevated at weaning in large intestinal digesta of piglets weaned at day 35 of age compared to piglets weaned at day 24 of age (P = 0.05). The weaning age in combination with the nutritional supplement had pronounced beneficial effect on gene expression of all investigated genes: interleukin-6, interleukin-10, nuclear factor kappa-beta, occludine, prostaglandin-endoperoxide synthase-2, tumor necrosis factor-alpha, and zonula occludens-1 (ZO-1) (P = 0.04). In conclusion, nutritional supplementation preweaning combined with increased weaning age could be considered as a strategy for improvement of the intestinal health, function, and maturation in piglets pre- and post-weaning, and a high CI enhanced piglets' robustness before weaning.

Effect of voluntary waiting period on metabolism of dairy cows during different phases of the lactation.

An extended calving interval (CInt) by extending the voluntary waiting period (VWP) could be associated with altered metabolism in dairy cows. The aim of this study was first to evaluate the effects of VWP on metabolism and body condition during the first 305 d after the first calving in the experiment (calving 1), around the end of the VWP, and during pregnancy (280 d before calving 2). Second, the effects of the VWP on metabolism were determined from 2 wk before until 6 wk after calving 2. Third, individual cow characteristics were used to predict milk production and body condition of cows after different VWP. Holstein-Friesian cows (N = 154, 41 primiparous [PP], 113 multiparous [MP]) were blocked for parity, milk production, and lactation persistency, randomly assigned to a VWP of 50, 125, or 200 d (VWP50, VWP125, or VWP200) and followed from calving 1 until 6 wk after calving 2. In the first 6 wk after calving 1 and from 2 wk before until 6 wk after calving 2, weekly plasma samples were analyzed for nonesterified fatty acids (NEFA), β-hydroxybutyrate, glucose, insulin, and insulin-like growth factor 1 (IGF-1). From wk 7 after calving 1 until 2 wk before calving 2, insulin and IGF-1 were analyzed every 2 wk. Fat- and protein-corrected milk (FPCM) and body weight (BW) gain were measured weekly. Cows were divided in two parity classes based on calving 1 (PP and MP) and remained in these classes after calving 2. During pregnancy, MP cows in VWP200 had greater plasma insulin and IGF-1 concentration and lower FPCM compared with MP cows in VWP125 (insulin: 18.5 vs. 13.9 µU/mL, CI 13.0-19.7, P < 0.01; IGF-1: 198.5 vs. 175.3 ng/mL ± 5.3, P = 0.04; FPCM: 22.6 vs. 30.0 kg/d ± 0.8, P < 0.01) or VWP50 (insulin: 15.8 µU/mL, P < 0.01; IGF-1: 178.2 ng/mL, P < 0.01; FPCM: 26.6 kg/d, P < 0.01) and had a greater daily BW gain compared with cows in VWP50 (3.6 vs. 2.5 kg/d ± 0.2; P < 0.01). After calving 2, MP cows in VWP200 had greater plasma NEFA concentration (0.41 mmol/liter) compared with MP cows in VWP125 (0.30 mmol/liter, P = 0.04) or VWP50 (0.26 mmol/liter, P < 0.01). For PP cows, the VWP did not affect FPCM or body condition during the first lactation in the experiment, or metabolism after calving 2. Independent of the VWP, higher milk production and lower body condition before insemination were associated with higher milk production and lower body condition at the end of the lactation. Variation in these characteristics among cows could call for an individual approach for an extended VWP.