Isopropanol Concentration Research Articles

Organic acid modified activated carbon filter media for removal of isopropanol in the cleanroom

Isopropyl alcohol (IPA) is commonly used for process cleaning. Expensive chemical filters are installed in cleanrooms to remove high concentrations of IPA contamination. We firstly use organic acid to modify coal-based activated carbon (AC) as a starting point for preparation of filtration media for IPA removal. AC was characterized before and after this modification using BET, SEM, thermogravimetric analysis (TGA), FTIR, and temperature programmed decomposition (TPD), comparing results with those obtained from the same tests applied to commercial coconut AC. These tests found that the specific surface area of coal-based AC, modified by the oxalic acid, was not as large as that of commercial AC, but there was a significant increase in surface area following surface modification. As can be seen from the SEM images, following modification, the surface roughness of the AC increases, with additional precipitation of oxalic acid particles. Besides the deposition of oxalic acid functional groups on the AC surface, modification also reduces the presence of basic groups and increases the number of acidic groups, as quantified by TPD with TGA. The adsorption isotherms of IPA adsorption are well fitted by both Langmuir and Freundlich isotherm models. Breakthrough times of 590, 413, and 380 days were observed for modified coal-based AC at 1 ppb initial IPA concentration and 0.3, 0.4, and 0.5 m s−1 face velocity, respectively. The adsorption kinetics are best described by a Pseudo-Second order kinetics model and also could be predicted by the intraparticle diffusion and membrane diffusion models.

Ultrafast Time-of-Flight Method of Gasoline Contamination Detection Down to ppm Levels by Means of Terahertz Time-Domain Spectroscopy

In this article, we present the possibility of using terahertz time-domain spectroscopy to detect trace, ppm-level (%wt.) concentrations of admixtures in 95-octane lead-free gasoline in straightforward time-of-flight (pulse-delay) measurements performed directly in the liquid. The method was tested on samples containing 75–0.0125% of isopropanol and 0.3–0.0250% of water. The detection limits for isopropanol and water content were determined to be 125 ppm and 250 ppm, respectively, approaching the limits of much more complicated and time-consuming methods (like spectroscopy of the vapor phase). The measured pulse delays were compared with theoretical calculations using the modified Gladstone-Dale mixing rule for the solutions. The comparison demonstrated good agreement for gasoline-alcohol mixtures and large discrepancies for gasoline-water mixtures, suggesting that gasoline-water mixtures cannot be considered idealized binary mixtures. Our results clearly show that the pulse time delay measurement by THz-TDS is a fast and sensitive method of gasoline contamination detection and, as such, can be easily integrated with industrial online real-time quality control applications.

Preparation and application of Co3O4 catalysts from ZIF-67 membranes over paper-like stainless steel fibers in isopropanol combustion

Co3O4 catalysts supported on paper-like stainless steel fibers (PSSF) were prepared by MOFs-templated (M) method and applied for catalytic combustion of isopropanol. Physicochemical properties of the as-synthesized Co3O4 catalysts were investigated by SEM, EDS mapping, TEM, XRD, BET, XPS and H2-TPR, respectively. Characterization results of SEM and TEM shown that Co3O4 (M)-350 and Co3O4 (M)-400 still remained the dodecahedron shape of the ZIF-67 precursor and possessed porous structures. The XPS results shown that the ratio of Co3+/Co2+ and Oc/Oc ​+ ​Oa ​+ ​Ol firstly increased and then decreased with the increase of calcination temperature. Among the as-synthesized catalysts, Co3O4(M)-400 exhibited the highest activity for the catalytic combustion of isopropanol with the T50 and T90 values of 258 ​°C and 281 ​°C, respectively, which was associated with high BET surface area, high amount of Co3+ species and abundant surface chemisorbed oxygen. Moreover, Co3O4 (M)-400 was employed as a structured catalyst for isopropanol combustion, the catalytic kinetics were studied based on different feed concentration, bed height and GHSV. The experimental results indicated that the T50 and T90 of isopropanol combustion over Co3O4(M)-400 with the bed height of 2 ​cm were increased slightly as the GHSV and feed concentration of isopropanol increased.

Application of near-infrared spectroscopy and multivariate methods for the estimation of isopropyl alcohol content in hand sanitizer formulation

Application of near-infrared spectroscopy and multivariate methods for the estimation of isopropyl alcohol content in hand sanitizer formulation

Fabrication of diverse multicompartment micelles by redispersion of triblock terpolymer bulk morphologies.

Redispersing block copolymer (BCP) bulk films in selective solvents is a simple and efficient method to prepare BCP micelles and polymersomes. While ABC triblock terpolymers are known to form multicompartment micelles (MCMs) with intricate nanoarchitecture, this is typically done by solvent exchange instead of redispersion of bulk films despite obvious advantages of greatly reduced solvent usage. Here, we provide guidelines on how to form MCMs with defined shapes and inner structure through direct redispersion of terpolymer bulk morphologies in selective plasticizing solvents. For this purpose, we redisperse a series of polystyrene-b-polybutadiene-b-poly(tert-butyl methacrylate) (PS-b-PB-b-PT) triblock terpolymers in acetone/isopropanol mixtures, where PT is always soluble, PB always insoluble, and PS will range from soft (high acetone content) to kinetically frozen (high isopropanol content). We investigate the effect of solvent mixtures, block composition, and thermal annealing on MCM shape and core morphology. Additionally, we performed terpolymer blend experiments to open up a simple route to further diversify the range of accessible MCM morphologies.

Determination of contaminants in artisanal cannabis products used for childhood epilepsy in the Australian community: A sub-analysis of the ‘PELICAN’ study

Despite recent approval of pharmaceutical-grade cannabis products for the treatment of childhood epilepsy, some families continue to use artisanal cannabis products as a way to manage seizures in their children. However, such products are typically of unknown composition and quality, and may therefore pose an unpredictable health risk to the child. In the present analysis, 78 samples of cannabis products collected (as part of a previous study) from families of children with epilepsy (average age 8.8 ± 4.6 years) were analyzed for heavy metals (arsenic, cadmium, lead, and mercury), residual solvents (panel of 19 solvents) and pesticides (panel of 57 pesticides). Due to small sample volumes obtained, only a subset of samples was used in each analysis. Results showed that no cannabis sample exceeded the toxicity limits for heavy metals (n = 51 samples tested). Of the 58 cannabis samples tested for residual solvents, 17 (29%) contained concentrations of ethanol or isopropanol above the generally accepted limit of 5000 parts per million. With the volumes consumed, it was thought unlikely that children were consuming hazardous amounts of residual solvents, although this could not be ruled out in every case. Most samples (n = 31 samples tested) yielded inconclusive results for the pesticides, although one sample contained concentrations of bifenthrin that were 4.9 times higher than the acceptable limit. Overall, these results highlight the need for improved access to quality-assured cannabis products and the education of doctors, patients, and artisanal manufacturers around the contaminant exposure risk in unregulated cannabis products.

Telomere length in peripheral leukocytes is a sensitive marker for assessing genetic damage among workers exposed to isopropanol, lead and noise: the case of an electronics manufacturer

BackgroundWorkers in electronics manufacturers may be exposed to various occupational hazards such as isopropanol, lead, and noise. Telomeres are special segments of cap-like DNA protein complex at end of liner chromosomes in eukaryotic cells. Telomere length is a potential marker of genetic damage. The aim of this study is to evaluate the effect of occupational hazards on the relative telomere length (rTL) of peripheral blood cells of workers in an electronics manufacturer, and to explore whether relative telomere length could be a biomarker for assessing genetic damage in the electronics manufacturing industry.MethodsWe investigated a large-scale electronics manufacturer in the Pearl River Delta Region. We ultimately collected 699 qualified workers (248 with isopropanol exposure, 182 with lead exposure, 157 with noise exposure, and 112 controls). During physical examination of the workers, we gave them questionnaires to understand their health statuses and living habits. We also collected peripheral blood samples from these workers to test exposure levels and rTL in the leucocytes.ResultsThe concentrations of air isopropanol in all monitored workshops was 25.3 mg/m3 and air lead smoke was 0.020 mg/m3. The maximum equivalent continuous A sound level noise exposure position was 82.2dB (A). All were lower than those in the Occupational Exposure Limits in Workplaces in China. Urinary acetone in the isopropanol exposed group was 1.04 (0, 1.50) mg/L, and cumulative urinary acetone was 1.48 (0, 5.09) mg-years/L. Blood lead levels (BLLs) were 28.57 (22.77, 37.06) µg/dL, and cumulative blood lead levels (CBLLs) were 92.75 (55.47, 165.13) µg-years/dL. rTL was different between occupational exposed workers and controls: rTL was 0.140 units (95 % CI: 0.022, 0.259) shorter in lead exposed workers and 0.467 units (95 % CI: 0.276–0.658) shorter in noise exposed workers compared to the controls. There is no statistical difference in rTL between isopropanol exposure workers and the controls. In order to elucidate the relationship between rTL and occupational hazards exposure, we divided the isopropanol exposure workers into three groups (0, ~1.43 mg/L, and >1.43 mg/L). None of the rTL difference was statistically significant among exposed workers at different uroacetone levels (P>0.05).The groups with ≥100 µg/dL blood lead had shorter rTL than the group with blood lead below 100 µg/dL (F=4.422, P=0.013). We incorporated age, gender, birthplace, race, education level, smoking, and alcohol consumption into the linear regression equation. Only blood lead concentration (X) was entered into the regression equation, yielding a multivariate linear regression equation of Y=0.397-0.124X (F=8.091, P=0.005). Workers with different hearing loss also had statistically significant differences in rTL (F=5.731, P=0.004). rTL was a protective factor for the occurrence of noise-induced hearing loss (NIHL). The longer the rTL, the lower the risk of NIHL [OR=0.64 (0.42, 0.98)].ConclusionsrTL was shorter in lead exposed workers and noise exposed workers, and it was a protective factor for the occurrence of the noise-induced hearing loss. Thus, rTL of peripheral blood may be a sensitive marker of genetic damage among workers in environments with lead and noise exposure.

Cellulose nanofiber separator for suppressing shuttle effect and Li dendrite formation in lithium-sulfur batteries

Lithium-sulfur battery (LSB) has high energy density but is limited by the polysulfides shuttle and dendrite growth during cycling. Herein, a free-standing cellulose nanofiber (CNF) separator is designed and fabricated in isopropanol/water suspension through vacuum filtration progress. CNFs with abundant polar oxygen-containing functional groups can chemically immobilize the polysulfides, and suppress the formation of the dendrites by controlling the surface morphology of the SEI on lithium metal in LSB. The isopropanol content in a suspension can fine-tune the pore structure of the membrane to achieve optimal electrochemical performance. The prepared separator displays integrated advantages of an ultrathin thickness (19 μm), lightweight (0.87 mg cm−2), extremely high porosity (98.05%), and decent electrolyte affinity. As a result, the discharge capacity of the LSB with CNF separator at the first and 100th cycle is 1.4 and 1.3 times that of PP separator, respectively. Our research provides an environmental-friendly and facile strategy for the preparation of multifunctional separators for LSBs.

Quantitative volatile organic compound sensing with liquid crystal core fibers

SummaryPolymer fibers with liquid crystals (LCs) in the core have potential as autonomous sensors of airborne volatile organic compounds (VOCs), with a high surface-to-volume ratio enabling fast and sensitive response and an attractive non-woven textile form factor. We demonstrate their ability to continuously and quantitatively measure the concentration of toluene, cyclohexane, and isopropanol as representative VOCs, via the impact of each VOC on the LC birefringence. The response is fully reversible and repeatable over several cycles, the response time can be as low as seconds, and high sensitivity is achieved when the operating temperature is near the LC-isotropic transition temperature. We propose that a broad operating temperature range can be realized by combining fibers with different LC mixtures, yielding autonomous VOC sensors suitable for integration in apparel or in furniture that can compete with existing consumer-grade electronic VOC sensors in terms of sensitivity and response speed.

Comparison of Effectiveness of Antiseptic Wet Wipe Products In Inhibiting Bacterial Growth With Replica Method

Hand washing is one of various way to maintain hand hygiene to prevent bacteria infections. Along with the development of the pharmaceutical and cosmetics industry appeared wet wipe products that are used as an alternative to handwashing. This study was conducted to determine antimicrobial activity in various samples of antiseptic wet wipe products that are easily obtained in the market in inhibiting the growth of E,coli, S.aureus , and C.albicans bacteria. Testing of antimicrobial properties is done using diffusion method, namely by inoculating the blank disc above so that Muller Hinton Agar that has been bred by bacteria and also replica test, namely by sticking the fingers that have been applied wet wipes on the media agar. The analysis was conducted by measuring the bland zones formed and calculating the number of bacterial colonies growing on the media. As a result, the sample of wet wipes C with isopropyl alcohol content of 70% which has the largest bland zone is 14 mm. In the replica test, only samples of wet wipes A grew bacteria on the media with an average number of 2 colony. In addition, the ANOVA One Way test was also conducted and obtained p > 0.05 which showed there was a significant difference in each treatment Keywords: Antiseptic wet wipes; Replica method; Antibacteria activity test

The Preparation of High Purity of Rebaudioside A and Stevioside and the Enrichment of Rebaudioside C by Subsequent Twice Crystallizations from Natural Stevia Resources

A comprehensive utilization of rebaudioside A (RA), stevioside (ST), and rebaudioside C (RC) from natural stevia resources was proposed. The influence of the solvent, solvent concentration, solid-liquid ratio, temperature, and time on the purity and recovery were investigated with response surface methodology. A 99% purity and 81% recovery of the RA were achieved by one crystallization of crude stevia under optimized conditions. Around a 95% purity and 80% recovery of the ST were easily achieved by the recrystallization of less value of mother liquor sugar (MLS) with a certain concentration of isopropyl alcohol–methanol aqueous solution. During the crystallization of the ST, the enriched RC in the liquid phase was more than three times higher than that in the crude stevia, which can provide cheaper RC raw material for the subsequent preparation of very expensive and high-purity RC.

Refining the in vitro release test method for a dapivirine-releasing vaginal ring to match in vivo performance

Previously reported in vitro release test methods for drug-releasing vaginal rings containing poorly water-soluble drugs have described use of water-alcohol systems or surfactant solutions in efforts to maintain sink conditions. Here, as part of efforts to more closely match in vitro and in vivo release for the 25 mg dapivirine matrix-type silicone elastomer vaginal ring for HIV prevention, we have investigated alternatives to the 1:1 v/v water/isopropanol medium described previously. Specifically, we evaluated dapivirine release from rings into (i) monophasic water/isopropanol mixtures of varying compositions and (ii) biphasic buffer/octanol systems using pH 4.2 and pH 7.0 buffers. The rate and mechanism of dapivirine release were dependent upon the isopropanol concentration in the release medium, in accordance with the observed trend in drug solubility. At 0 and 10% v/v isopropanol concentrations, dapivirine release followed a partition-controlled mechansim. For media containing ≥ 20% v/v isopropanol, in vitro release of dapivirine was significantly increased and obeyed permeation-controlled kinetics. Cumulative release of ~3.5 mg dapivirine over 28 days was obtained using a water isopropanol mixture containing 20% v/v isopropanol, similar to the ~4 mg dapivirine released in vivo. Dapivirine release into the biphasic buffer/octanol system (intended to mimic the fluid/tissue environment in vivo) was constrained by the limited solubility of dapivirine in the buffer component in which the ring resided, such that cumulative dapivirine release was consistently lower than that observed with the 20% v/v isopropanol in water medium. Release into the biphasic system was also pH dependent, in line with dapivirine’s pKa and with potential implications for in vivo release and absorption in women with elevated vaginal pH.Graphical abstract

Biocatalytic preparation of a key intermediate of antifungal drugs using an alcohol dehydrogenase with high organic tolerance

In this study, an alcohol dehydrogenase derived from Lactobacillus kefir (LkADH) was engineered and a simple and practical bioreduction system was developed for the preparation of (R)-2-chloro-1-(2, 4-dichlorophenyl) ethanol ((R)-CDPO), a key intermediate for the synthesis of antifungal drugs. Through active pocket iterative saturation mutagenesis, mutant LkADH-D18 (Y190C/V196L/M206H/D150H) was obtained with high stereoselectivity (99% ee, R vs 87% ee, S) and increased activity (0.44 µmol·min−1·mg−1). LkADH-D18 demonstrated NAD(P)H regeneration capability using a high concentration of isopropanol (IPA) as a co-substrate. Using 40% IPA (v/v), 400 mM of (R)-CDPO (90.1 g·L-1) was obtained via complete substrate conversion using 40 mg·mL−1 LkADH-D18 wet cells. The biocatalytic process catalyzed at constant pH with the cheap co-solvent IPA contributed to improved isolated yield of (R)-CDPO (97%), lower reaction cost, and simpler downstream purification, indicating the potential utility of LkADH-D18 in future industrial applications.

Impact of isopropanol on nucleation and growth of polyaniline nanofibers towards capacitive charge storage enhancement

The nanofibrous morphology and chain orientation have predominant impacts on the charge storage ability of polyaniline (PANi) that could be promoted by introducing small molecules such as alcohols to affect the formation of the nanofibers. In this work, one kind of continuous–flow polymerization was designed to separate the seed formation and subsequently assembly process of PANi nanofibers, making it possible to evaluate independently the influence of isopropanol on the nucleation and growth of PANi nanofibers, i.e. isopropanol molecules were involving in the initial reaction in the flow polymerization, or only in the subsequent assembly and growth of nanofibrous seeds produced through flow polymerization. Furthermore, the effects of isopropanol content on the morphologies, microstructures and capacitance performances of PANi nanofibers were discussed. It is found that the isopropanol could give a great improvement in charge storage ability of the nanofibers when a small amount of alcohol molecules were only used for affecting the assembly and growth of PANi nanofibers. Benefiting relatively large diameter–to–length ratio and high conductivity, PANi nanofibers prepared at 5 wt. % content of isopropanol possessed the greatest specific capacitance of 1082 F g–1 at the scan rate of 1 mV s–1 and 530.2 F g–1 at the current density of 1 A g–1, which was improved by 10% compared with pure PANi. The understanding of effect of small organic molecules on the capacitance performances of PANi could provide a much easier route in fabricating high performance electrode of PANi as promising candidates for lightweight and flexible energy storage devices.

A novel isopropanol salt aqueous two-phase system and its applications for 2, 3-butanediol extraction

At present, the hydrophilic alcohol and inorganic salt combined with the two-phase system have been widely used in the extraction of biological fermentation products. However, the residual salt in the extraction solution of the existing system caused the loss of salt and increased the energy consumption of subsequent product separation. Therefore, how to achieve organic phase without salt, water phase without extractant is particularly important. This paper studies the ternary liquid-liquid equilibrium of four highly soluble inorganic salts (K4P2O7, K2HPO4, K3PO4, K2CO3) + isopropanol + water at 298.15 K and their applications. By comparison, the extraction system with no salt and low water content in the organic phase and no extractant in the aqueous-rich phase was selected, with the K4P2O7/isopropanol aqueous phase system being the best system. Moreover, the screened system avoided the overuse of extraction solvent. The results showed that the isopropanol content had little effect on the water content in the extract phase. The salting-out extraction recovery rate of 2,3-butanediol is 100%, and the concentration coefficient is 5.74, which is much larger than other systems. More importantly, no salt was in the extract phase and no isopropanol was in the water-rich phase. Biofuel recovery from fermentation broth by K4P2O7/isopropanol aqueous two-phase system has the advantages of high selectivity and can effectively reduce energy demand.

Artificial Intelligence based modeling of pervaporation process for alcohol dehydration

In this study, an Artificial Intelligence/artificial neural network (ANN) based approach is used to model the pervaporation process for alcohol dehydration using an inorganic membrane. The Deep Learning Toolbox of MATLAB was used to model an artificial neural network. ANN was developed with a single hidden layer and a variable number of neurons. Results showed that minimum Mean Squared Error was found with 12 neurons for permeate flux and 15 neurons for selectivity, and thus, the same number of neurons were considered for further simulations. Model predictions showed a good fit with experimental results with 0.96 and 0.98 correlation factors for permeate flux and selectivity, respectively, which provide confidence in the model predictions.Later, trained neural networks were used to study the effects of operating parameters. A maximum value of flux 5.8 kg/m2h was found at 90 °C feed temperature and 2.9 kPa permeate pressure. Similarly, the highest selectivity for water 1099 was found at 100 dm3/h flow rate and 0.96 kg/kg isopropanol concentration.

Gaseous isopropanol removal in a microbial fuel cell with deoxidizing anode: Performance, anode characteristics and microbial community

A deoxidizing packing material (DPM) with an encapsulated deoxidizing agent (DA) was developed to construct the packed anodes of a trickle-bed microbial fuel cell (TB-MFC) for treating waste gas. The encapsulated DA can consume O2 in waste gas and increase the voltage output and power density (PD) of the constructed TB-MFC. The DPM effectively enables the circulating water in TB-MFC for maintaining a low level of dissolved oxygen for 80 h. The results revealed that when the concentration of isopropanol (IPA) in waste gas was 0.74 g/m3, the TB-MFC (DPM with DA) exhibited an IPA removal efficiency (RE) of up to 99.7%. When DPM with DA was used as the packing material of the TB-MFC (486.6 mW/m3), the PD was 2.54 times that obtained when using coke as the packing material (191.6 mW/m3). The next-generation sequencing results demonstrated that because the oxygen content of the MFC anode chamber decreased over time in the TB-MFC, the richness of anaerobic electrogens (Pseudoxanthomonas, Flavobacterium, and Ferruginibacter) in the packing materials was increased. These electrogens mainly attached to the DPM, and IPA-degraders appeared in the circulating water of the TB-MFC. This enabled the TB-MFC to simultaneously achieve a high voltage output and IPA RE.

A real-time sharp selectivity with In2S3 gas sensor using a nonlinear dynamic response for VOCs

Selectivity continues to be one of the major challenges for gas sensors. This work reports on transient variations in the resistance of In2S3 sensors following injection of different concentrations of ethanol, methanol, isopropanol, acetone and toluene at 350 °C operating temperature. Volatile Organic Compounds (VOCs) are recognized using the transient evolution of a single sensing element that offers different characteristics that can be direct or indirect signals detection of the target gas. Novel features for twenty individuals were processed by principal component analysis and hierarchical ascending classification, and four obvious clusters were observed. The output of the VOC detection sensor for the gaseous analyte is represented by the result of analyzes of three data matrices having 9, 7 and 8 dimensional vectors. Result is delivered with a sequence of three digits determined after global execution. Based on this approach, the distinction of one reducing gas from another is demonstrated.

Removal of Isopropanol by synergistic non-thermal plasma and photocatalyst

The dielectric barrier discharge (DBD) of non-thermal plasmas was combined with a self-made photocatalyst to remove isopropanol (IPA). Synthesis conditions for the novel photocatalyst, including calcination temperature and copper loading, were varied before photocatalysis to obtain at the optimal reaction efficiency. The effects of initial IPA concentration, oxygen content, and catalyst dosage were also observed. Finally, catalyst reusability was analyzed. X-ray photoelectron spectroscopy fitting revealed Ti, Cu, C, and O peaks in the synthesized catalyst. After a 60-min reaction with 100% oxygen as the carrying gas, nearly 100% of the IPA was converted. Overall, the optimal IPA conversion efficiency and acetone and carbon dioxide selectivity were achieved when the photocatalyst was synthesized at a calcination temperature of 550 °C and copper loading of 1.8%, along with a 100% oxygen carrying gas and a 3-mm discharge gap.

In-vitro evaluation of bactericidal activity of antiseptics and disinfectants commonly used in healthcare settings

Introduction: Healthcare-associated infections are common problems found in healthcare systems in most of the countries. Proper use of antiseptics and disinfectants is useful in reducing the magnitude of such infections. The aim of this study was to evaluate the bactericidal effect of different concentrations of selected antiseptics and disinfectants. Methods: Bactericidal activity of different concentrations of antiseptics and disinfectants; isopropyl alcohol, povidone iodine, chlorhexidine gluconate, sodium hypochlorite, hypertonic saline, peracetic acid and mixture of 2-aminoethanol, dodecyl dimethyl ammonium chloride, potassium carbonate & bis(3-aminopropyl) dodecylamine were evaluated against nine strains of American Type Culture Collections and 11 strains isolated from the hospitals. Bacterial suspensions were prepared equal to 0.5 McFarland turbidity standards and 100 μl of each was mixed with 3 ml of antiseptic/ disinfectant solution. After desired contact time (1 minute, 15 minutes and 24 hours), 20 μl of reaction mixer was transferred into 4 ml of sterile physiological saline, mixed well and 20 μl of solution was evenly spread throughout Muller Hinton agar/ blood agar plates. Growth control was done using 3 ml of distilled water instead of 3 ml of antiseptic/ disinfectant solution. All plates incubated at 35oC ± 2oC for 18 – 24 hours. Colonies were counted and reduction percentage was calculated. Results: All concentrations of isopropyl alcohol, povidone iodine, chorhexidine, sodium hypochlorite, peracetic acid and the mixture of 2-aminoethanol, didecyldimethylammonium chloride, potassium carbonate and bis(3-aminopropyl)dodecylamine showed 100% bactericidal activities against all tested bacterial strains in1-minute contact time. Bactericidal activity of hypertonic saline was varied against tested bacterial strains with higher bactericidal activity against Gram positive organisms than Gram negative organisms. Conclusions: Currently using concentrations of antiseptics and disinfectants evaluated in this study have 100% bactericidal activity other than hypertonic saline.