Concentration Of Coconut Water Research Articles

Study the Efficacy of Different Concentrations of Coconut Water on Boar Semen Following Equilibration at 18°C for Different Hours

Study the Efficacy of Different Concentrations of Coconut Water on Boar Semen Following Equilibration at 18°C for Different Hours

Pollination Compatibility of Dendrobium Polinela Lampung, and The Effect of Media Composition with Coconut Water on Seed Germination In Vitro

Efforts to increase the quality of Dendrobium orchids can be made by increasing the genetic quality through crosses while increasing the quantity can be done by propagation through in vitro culture. Information about the compatibility of an orchid species is very important to maximize the acquisition of seeds as propagation material. The seeds from the cross were propagated by in vitro culture technique. The purpose of this study was to obtain a compatible crossing method between crossing and selfing D.Polinela Lampung orchids with selected parents and to determine the effect of giving a combination of media with coconut water concentration in inducing germination and growth of orchid seeds. This study consisted of two experiments, namely (1) the compatibility test of D.Polinela Lampung orchids with three types of Indonesian orchids (D.discolour, D.leporinum, D.lasientera), and (2) the effect of basic media (MS, VW, Growmore, Gandasil) and water concentration CW (0, 50, 150, and 250 ml/1) on seedling growth of D.Polinela Lampung x D.lasientera orchids. In conclusion, among the sixteen crosses of Dendrobium orchids, ten compatibility crosses were found (pollination that produces fruit). The addition of 15 and 25% CW in the growmore medium (91.37% and 98.03%), MS (90.97% and 96.00 %), and Gandasil (90.67% and 92.67%) gave the highest percentage results. Seed germination and protocorm formation. The protocorm that forms the first leaves produced on Growmore media added with 50-150 ml/1 coconut water gave the most results compared to other treatments. The results of the research are expected as basic information in orchid propagation, especially the study of compatibility and germination of orchid seeds.

The Effect of Natural PGR Combination on The Growth of Fig (Ficus carica L.) Plant Cuttings

Fig is shrub with short stems, creeping, not many trunks and woody which have cambium tissue due to thickening between the xylem and phloem, can grow and bear fruit in the hot sun or in the desert. Fig plant is a plant that has many benefits. The purpose of this research is to determine the effect of natural Plant Growth Regulator (PGR) (mature coconut water, young coconut water and shallot extract) and obtain the best concentration of PGR for Fig plant cuttings. The study was carried out by field trial methods arranged in Completely Randomized Design (CRD) with a single factor trial design. The treatments tested were the addition of PGR from natural ingredients, namely: mature coconut water 20% and 30%, young coconut water 20% and 30%, red shallot extract 60% and 70% and commercial PGR (Root-Up™™). Each treatment was repeated 3 times. The results of this search showed that all-natural PGR tested, namely from young coconut water concentrations of 20% and 30%, mature coconut concentrations of 20% and 30% and shallots concentrations of 60% and 70% can be used to increase the growth of Fig plant cuttings. All natural PGRs tested, namely young coconut water with a concentration of 20% and 30%, mature coconut with a concentration of 20% and 30% and shallots with a concentration of 60% and 70% can be used to increase the growth of fig plant cuttings.

PENGARUH PENAMBAHAN EKSTRAK JAGUNG MANIS DAN AIR KELAPA PADA MEDIA MS SECARA IN VITRO TERHADAP INDUKSI TUNAS ADVENTIF BAWANG PUTIH (Allium sativum L.)

[SWEET CORN EXTRACT AND COCONUT WATER EFFECT TO INDUCE GARLIC (Allium sativum L.) ADVENTIST SHOOTS BY IN-VITRO CULTURE]. Garlic (Allium sativum L.) is one of the horticultural plants used daily for various uses. This study aims to determine the best extract concentration of sweet corn and coconut water and its interaction for induction garlic Adventist shoots (Allium sativum L.) var. Lumbu Hijau. The study was conducted at the Biotechnology Laboratory, Agriculture Faculty, UPN ”Veteran” East Java, in February – April 2021. The experimental design used was a factorial completely randomized design (CRD). The first factor is four levels of extract of sweet corn concentration (0, 50, 100, and 150 g/L), while the second factor is four levels of coconut water concentration (0, 50, 100, and 150 mL/L). The results showed that the extract of sweet corn concentration-time treatment increased the growing adventist shoots percentage, adventist shoots number, and adventist shoots size. The section of sweet corn concentration of 150 g/L increased the rate of growing adventist shoots, adventist shoots number, and adventist shoots size. The treatment of coconut water concentration and its interaction with the extract of sweet corn concentration is still not able for induction garlic adventist shoots (Allium sativum L.) of the var. Lumbu Hijau.

Production of potato (Solanum tuberosum) pre-basic seed through tissue culture in Katibougou, Mali

Abstract. Abdoulaye M, Blay ET, Eleblu JSY. 2021. Production of potato (Solanum tuberosum) pre-basic seed through tissue culture in Katibougou, Mali. Cell Biol Dev 5: 90-104. In Mali, the main problem limiting the productivity and production of potatoes is the non-availability of quality seeds in adequate quantities and at affordable prices. This study proposes two experiments on the techniques adopted in Mali's IPR/IFRA plant biotechnology laboratory to meet quantity and quality improvements. That evaluation of the effects of 3 concentrations of coconut water and 2 of potassium nitrate on potato plantlets growth in vitro and of 2 physiological ages and 3 substrates on potato in vitro plants' establishment, post in vitro growth, and mini tubers production in vivo. The first experiment was a Completely Randomized Design (CRD) with 12 treatments replicated 4 times. The second was a factorial experiment with 2 factors (physiological ages: 2 levels and substrate: 3 levels) in a Randomized Complete Block Design (RCBD) with 6 treatments replicated 4 times. The first experiment showed that the culture medium M7 (MS+40 mL/L of coconut water +250 mg of potassium nitrate) had promoted all plant growth parameters (shoot emergence, plant height, number of nodes, leaves and roots, and plant fresh and dry weight) after 30 days of in vitro culturing. The lower concentrations of coconut water (40 mL) and potassium nitrate (250 mg) per liter of MS medium had significant and positive effects on all the in vitro growth parameters after 30 days. The second experiment showed that the plantlet weaning age of 25 days and the post-flask culture substrate S1 (only soil) provided the best plant survival percentage at 20 days after transplanting in vivo. The substrate S2 (soil and cow dung 2:1) positively affects plant stem length, stem diameter, fresh and dry biomass formation, tuber yield, tuber numbers per plant, and tuber grading size B (tubers with a diameter of less than 28 mm). The substrate composition S1 (only soil) has significantly affected the weight loss (12.50%) of tubers stored within 8 weeks. The weaning age was 45 days, and the substrates S2 (soil and cow dung 2:1) and S3 (soil and cow dung 1:1) significantly reduced the number of sprouts per tuber and sprouts number per eye on tubers. In addition, the results indicate that for the better and more rapid growth of potato plantlets in vitro culture, the coconut water concentration used as a supplement to MS medium should be 40 mL per liter of medium. The proportion of cow dung used in substrate composition should not exceed the soil and cow dung ratio 2:1 for maximum post-transplanting plant re-establishment rate in vivo and rapid maturity of mini-tubers.

38 The efficacy of different concentration of coconut water on boar semen following equilibration at 18°C for different times.

Reproduction, Fertility and Development is an international journal publishing original research , review and comment in the fields of reproduction and developmental biology in humans, domestic animals and wildlife

Study of Making Yogurt From Peanut (Arachis hypogaea L.) With The Addition of Coconut Water and Skim Milk

Abstract- Plant-based functional foods has been highly developed in recent years. Peanut (Arachis hypogaea L.) can be used as functional drink that substitute for cow's milk. This study aimed to determine the effect of ratio (peanut milk and coconut water) and skim milk concentration on the quality of peanut yogurt. Yogurt was made by mixing peanut milk with coconut water and adding skim milk. The ratio of peanut milk and coconut water (50% : 50% and 75% : 25% v/v) and skim milk concentration (0%, 1% and 2% w/v). Fermentation was carried out at room temperature for 12 hours using commercial yogurt starter culture. Peanut yogurt analysis included of total LAB, pH, total acid and organoleptic. The data obtained were tabulated and presented descriptively. The best peanut yogurt was obtained in the ratio of peanut milk and coconut water (75%: 25%) and the addition of 2% skim milk which showed total LAB, pH and total acid were 9.2 x 109 CFU/mL, 2.8 and 0.37% respectively.

Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

Seed Priming Used Coconut Water In Different Concentration And Soaked Periode To Increase Soybean Germination

Soybean seeds are orthodox seeds that quickly deteriorate, especially if the storage environment conditions are less favorable (sub optimum). Soaking using coconut water can be done to overcome the deterioration of soybean seeds by utilizing vegetable waste. The purpose of this research was to study the effect of concentration and duration of coconut water soaking on the process of soybean seed germination. This research was conducted in January-February 2020, at the Agronomy Laboratory, Agrotechnology Study Program, Faculty of Agriculture-Livestock. The soybean used in the study was the Dena I variety. Factorial Completely Randomized Design (CRD) with 2 factors was used in this study. Factor 1 was coconut water concentration (K0 = 0% Coconut Water Concentration; K1 = 25% Coconut Water

Penggunaan Air Kelapa Muda Sebagai Zat Pengatur Tumbuh Terhadap Daya Kecambah, Vigoritas, Berat Kering Biji Tanaman Kelor (Moringa oleifera)

The more common methode for planting moringa plant is using seed, because this plant produces a lot of seeds. However, the resulting seeds have a hard enaugh seed coat so that. These conditions can affect and even thwart germination, vigor and growth of the plant. This Study aims to determine the proper dosage of young coconut water to be used in growing moringa seeds, so that later it become a recommendation for formers in the development of moringa plants. This research was conducted using a green house and laboratory scale method with the media that had been prepared for germination obsevations were carried out for two weeks to obtain data according to the observed parameters which included capacity, Vigority and dryweight of moringa plants. The result of the analysis of variety showed that the concentration of young coconut water had no significant effect (P>0.05) on the germination, vigor and dryweight of moringa plants. Likewise, the interaction between immersion time and coconut water concentration had no significant effect (P>0.05) on the observed variables. This situation is due to the fact that not all moringa seeds undergo the imbibition process properly so that they effect the observed variables.

Media Tanam Kultur Jaringan yang Tepat untuk Perbanyakan Tanaman Pisang Cavendish (Musa acuminata L.)

There are many types of media used in tissue culture, one of which is Murashige and Skoog (MS). Growth regulators commonly used in tissue culture include Indol Acetic Acid (IAA), Naphthalene Acetic Acid (NAA), Benzyl Amino Purine (BAP), and Indole 3-Butyric Acid (IBA). The aim was to examine the effect of various types of media treatment on the growth of Cavendish banana plants. This research method was carried out by reviewing relevant articles using google until mid-2020. Based on the results of the discussion, it was shown that the most appropriate media for the process of propagation of the Cavendish banana plant was media combined with BAP and IAA hormones, because the concentrations of BAP and IAA hormones could be used. Spur the process of organogenesis in banana plantlets. In addition, giving Super Growth foliar fertilizer 1 ml / l can also stimulate root growth at the rooting stage, so that giving Super Growth is sufficient to induce the formation of shoots and plant roots. Giving coconut water concentration of 7.5% and 15% into the planting medium can produce the best shoot initiation and the number of banana shoots.

RESPON EKSPLAN PISANG KLUTUK (Musa paradisiaca L.) TERHADAP KONSENTRASI EKSTRAK BIJI PINANG MUDA DAN AIR KELAPA MUDA SECARA IN VITRO

Nurseries play an important role in banana development. The tissue culture technique is an alternative to produce quality seeds in large quantities, uniform and produced in a short time and free of pathogens. Areca nut extract is an organic material that contains tannin and can replace bicycling used as a disinfectant in tissue culture activities. Coconut water is a food reserve that contains vitamins and growth substances, so it can stimulate germination. The purpose of the study was to examine the interaction and single response of klutuk banana explants to the concentration of areca nut extract and young coconut water in vitro. This research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, the Islamic University of Riau with the planting material used Pisang Klutuk weevil with the method used in a factorial Completely Randomized Design (CRD) with 4 x 4. The first factor was seed extract Young areca nut and the second factor was Young Coconut Water. The parameters observed were the percentage of live explants, the percentage of contaminated explants, the age of shoot emergence, and the number of shoots. The observation results were analyzed statistically and continued with the further test of Honest Significant Difference (HSD) at the 5% level. It was found that the best treatment was P2K2 (20% young betel nut extract (200 ml + 800 ml distilled water) and young coconut water 20 ml/liter media), where the percentage of life was 100%, the percentage of contamination was 0%, the age of shoots appeared was 37.67 days, and the number of shoots was 5 strands.

PEMATAHAN DORMANSI BENIH KELAPA SAWIT (Elaeis guinensis Jacq.) MENGGUNAKAN KALIUM NITRAT (KNO3) DAN AIR KELAPA

The aim of this study were, 1) to find out and study the effect of KNO3 immersion treatment on oil palm seed germination; 2) to find out and study the effect of coconut water immersion treatment on oil palm seed germination; 3) to find out and study the interactions that occur between KNO3 immersion treatment and coconut water to oil palm seed germination. This research was conducted in July 2019 until August 2019 in the Laboratory of the Department of Agriculture Cultivation, Faculty of Agriculture, Palangka Raya University using a Completely Randomized Design with 2 factors. The first factor is the concentration of KNO3: K0 (0%); K1 (0.2%); K2 (0.4%); K3 (0.6%) and the second factor is coconut water concentration, B0 (0%); B1 (60%); B2 (80%); B3 (100%). The parameters observed were first count germination, maximum growth potential, germination power, growth speed, radicle length, plumular length and dormancy intensity. The results showed that the treatment of KNO3 concentration significantly affected first count germination, maximum growth potential, germination rate, growth speed and dormancy intensity. Treatment of coconut water concentration significantly affected first count germination, maximum growth potential, germination rate, growth speed and dormancy intensity. The interaction between the treatment of KNO3 concentration and coconut water concentration had no significant effect on all observed parameters

AKUMULASI BAHAN KERING DAN INDEKS KLOROFIL BIBIT KAKAO AKIBAT APLIKASI AIR KELAPA DENGAN KONSENTRASI BERBEDA

Cocoa seedling are plant material to be planted in the field, superior seedling can produce maximum cocoa pods. Good cacao seeds can be produced from superior cultivar, one of the superior cultivar was the ICCRI 08H cultivar. Fertilization was required so that the cocoa seeds can grow perfectly. The fertilizer was used usually inorganic fertilizers, there was an alternative to fertilizing using coconut water. The purpose of this study was to see the results of photosynthesis reflected in the dry weight due to coconut water in various concentrations. The research was conducted at the Experimental Garden of the Ciparanje Faculty of Agriculture, Jatinangor in October 2018 until March 2019. The experiment used a randomized block design (RBD). The treatments used were several concentrations of coconut water and their combination with inorganic fertilizers. The results showed that the dry weight accumulation of cocoa seedlings was influenced by various concentrations of coconut water, but had no effect on the chlorophyll index. The concentration of coconut water of 50% is the optimum concentration for better dry matter accumulation of cocoa seedlings and can be an alternative fertilizer for cocoa seedlings.

THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

The use of coconut water (cocos nucifera l.) as alternative media to substitute Sabouraud Dextrose Agar (SDA) for the growth of aspergillus flavus

Media is a material composed by mixed nutrition to grow microorganisms, common media to grow fungi is Sabouraud Dextrose Agar (SDA). It contains 10 g of epton, 40 g of dextrose and 15 g of agar. This study used various concentration of coconut water (20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. The aim of this study is to determine the concentration of coconut water that can be used to replace Sabouraud Dextrose Agar (SDA) for the growth of Aspergillus flavus and to determine the difference of colony diameter of Aspergillus flavus and to determine the difference of colony diameter of Aspergillus flavus. Macroscopic observation of colony diameter (mm) has been done in 1 x 24 hours for 10 days using calipers with Lactophenol Cotton Blue (SDA) dyes for affirmation test. Data was analyzed statistically using One Way Anova continued by Post hoc test. It showed that the higher the concentration of coconut water, the bigger the diameter colony (sig. 0,00 < 0,05). Post hoc test showed that coconut water concentration of 20%, 30%, 40%, 50%, and 60% gave lower significant growth of the fungus than Sabouraud Dextrose Agar (SDA) media, while the concentration of 70%, 80%, and 90% gave non – significant value or similar to wards control media Sabouraud Dextrose Agar (SDA). The concentration it can be concluded that coconut water can be used as an alternative media to substitute SDA (Aspergillus flavus) for the growth of Aspergillus flavus.

RESPON PERTUMBUHAN DAN PRODUKSI BAWANG MERAH (Allium cepa L) TERHADAP KONSENTRASI AIR KELAPA DAN MEDIA TANAM SECARA VERTIKULTUR

The study was conducted at experimantal field at Faculty of Agriculture, Asahan University, Asahan Regency on October 2020 until December 2020. The study aims to the find out the growth response and production of shallots (Allium cepa L.) to the concentration of coconut water and growth media veticulture. This study used randomized complete block design with two factors: (1) Concentration of coconut water (K) with 3 levels: K1 = 25% (250 ml coconut water + 750 ml water), K2 = 50% (500 ml coconut water + 500 ml water), K3 = 75% (750 ml coconut water + 250 ml water) and (2) growth media (M) with 3 levels: M1 = soil + cow manure (1:1), M2 = soil + cow manure (2:1), M3 = soil + cow manure (3:1). Observation parameters were plant height, number of leave, number of bulbs, bulbs weight. The results showed that coconut water concentration had a significant effect on the number of tubers per clump and tuber wet weight, but had no significant effect on observations. plant height and number of leaves, the best treatment at K3 = 75% (750 ml coconut water + 250 ml water). The planting medium did not significantly affect the observations of plant height, number of leaves, number of tubers per clump and wet weight of tubers. The interaction between the concentration of coconut water and growing media did not have a significant effect on all observed variables.

Comparation Between Mac conkey and Coconut Water Medium as a Growth Medium for Escherichia coli

Escherichia coli is the bacteria that can cause diarrhea in humans and often used as a parameter of stool environmental pollution. Culture of E. coli from the sample often requires Mac Conkey as commercial media which is able to distinguish it from other bacteria in the Enterobacteriaceae group. Commercial media such as Mac Conkey certainly has a price that is quite expensive because of its ability as a growth medium for Enterobacteriaceae. Therefore, in the study tested natural ingredients that can be used for growth media, such as coconut water. The purpose of this study was to compare the ability of Mac Conkey media and coconut water to support the growth of E. coli. This research is an experimental study with a completely randomized design. The concentration of coconut water tested was 0%, 20%, 40%, 60%, 80%, and 100%. The results showed that at the concentration of coconut water 20% to 60% the number of E. coli colonies on coconut water media was slightly below the Mac Conkey Agar media, while in coconut water a concentration of 80% showed a greater number of colonies than Mac Conkey. The Mann Whitney test showed a significant difference between the number of colonies on 80% coconut water media and Mac Conkey Agar, which was equal to 0.004 (p &lt; 0.05). Based on these results, coconut water has the potential to be used as a growth medium for E. coli.

Potato tuber (Solanum tuberosum L.) formation due to the application of different concentrations of coconut water in in-vitro

This study apply coconut water to support the process of formation of potato tubers in vitro. The study was conducted at the laboratory of tissue culture, Experimental Garden of Vegetable Research Center, Berastagi, North Sumatera. The experiment used Completely Randomized Design with single treatment factor, the coconut water concentration of 0; 75; 150 and 225 ml L−1. The results show that the application of coconut water provides a significant effect on the number, diameter and fresh weight of potato micro tubers, but the effect is not significant at the time of potato micro tuber formation. The application concentration of coconut water 225 mL L−1 affected the potato micro tuber formation in in vitro.