Compounds In Sprouts Research Articles

Comparative Analysis of Polyphenolic Profile and Chemopreventive Potential of Hemp Sprouts, Leaves, and Flowers of the Sofia Variety.

This study investigates the phytochemical composition and biological activities of hemp (Cannabis sativa L.) leaves, flowers' methanolic extracts from the Sofia variety, and its sprouts cultivated under different light conditions (natural light, darkness, blue, and white LED light for 5, 7, and 9 days). Phytochemical analysis using HPLC identified four key polyphenolic compounds in sprouts' extracts: chlorogenic, caffeic, and gallic acids, and myricetin, with a predomination of the chlorogenic acid. In contrast, leaves and flowers' extracts contained cannflavins A and B and chlorogenic, p-coumaric, and ferulic acids, with a significant presence of isochlorogenic acid. Antioxidant capacity, assessed by FRAP method, revealed higher antioxidant potential in leaves compared to flowers and sprouts, with sprouts grown under blue and white LED lights exhibiting the highest activity. Cytotoxic activity was evaluated on human colon cancer cell lines (HT29, HCT116, DLD-1) and normal colon epithelial cells (CCD 841 CoN). Results demonstrated significant and selective cytotoxicity against cancer cell lines, with leaves showing more pronounced effects than flowers, and sprouts only moderate activity. All samples revealed an anti-inflammatory effect in vitro. To conclude, sprouts, leaves, and flowers of the Sofia hemp may be considered promising products for chemoprevention in the future.

Simultaneous high-performance liquid chromatography analysis of anthraquinones in sicklepod sprouts with α-glucosidase inhibitory activity.

Sicklepod [Cassia obtusifolia L. syn Senna obtusifolia (L.) H.S. Irwin & Barneby, Fabaceae] sprouts are promising ingredients with health-promoting benefits. Notwithstanding, the pharmacologically active compounds in sicklepod sprouts have not been studied or analysed in detail. This study aimed to isolate and structurally identify phytochemicals showing α-glucosidase inhibitory activity in sicklepod sprouts and simultaneously quantify the compounds in the sprouts to determine the optimal cultivation method and germination time to maximise active compounds. A simultaneous high-performance liquid chromatography-ultraviolet (HPLC-UV) method with high sensitivity and accuracy was developed and used to analyse time-dependent changes in anthraquinone content during sicklepod germination. Thirteen anthraquinones were isolated and identified, of which six-chrysoobtusin, emodin, 1-O-methyl-2-methoxychrysophanol, 7-O-methylobtusin, chrysophanol, and physcion-showed moderate α-glucosidase inhibitory activity. The maximum content of anthraquinones in a sprout was observed on Day 5 under both light and dark conditions. The findings of this study revealed that sicklepod sprouts which are promising functional food materials contain a variety of anthraquinones.

ZnO nanoparticles synthesized by chemical precipitation to increase germination and bioactive compounds in sprouts of Raphanus sa-tivus L.

Objective: To examine how priming radish (Raphanus sativus L.) sprouts with zinc oxide nanoparticles (NPs-ZnO) affects their germination, photosynthetic pigments, phenolic compounds, and zinc content. Design/methodology/approach: We evaluated five NPs-ZnO treatments and a control sample with four replications under a completely randomized design. Results: Sprouts treated with NPs-ZnO showed increased germination variables, photosynthetic pigments, phenolic compounds, and zinc content as compared to untreated radish sprouts. Study limitations/implications: It is hard to establish a response model for the effects of NPs since their shape, size, surface charge, chemical composition, and concentration may have a differentiated impact on seed germination. Findings/conclusions: Using NPs-ZnO could be an effective way to enrich crops, since the passage of Zn through plant tissues will cause an accumulation of this micronutrient.

Preliminary Study on the Impact of Non-Thermal Plasma Activated Water on the Quality of Triticum aestivum L. cv. Glosa Sprouts

The present study aimed to investigate the effects of non-thermal plasma-activated water (PAW) on Triticum aestivum L. cv. Glosa sprouts. Two types of PAW were generated by exposing distilled water to a high-voltage electric discharge. Wheat caryopses were treated either with PAW1 (25 mg/L NO3−, 4 mg/L NO2−, and 6 mg/L H2O2) or PAW2 (35 mg/L NO3−, 5 mg/L NO2−, and 7.5 mg/L H2O2) for 8 days, with samples being collected at days 1, 2, 3, and 8. The germination rate, growth parameters, protein, photosynthetic pigments, total phenolic contents, antioxidant activity of free and bound phenolic fractions, and activity of antioxidant enzymes were evaluated. Both PAW1 and PAW2 had positive effects on Triticum aestivum L. cv. Glosa sprouts. PAW2 had a better impact on sprouts with respect to growth parameters, free phenolic content, and antioxidant activity of the free phenolic fraction (day 3), the accumulation of proteins, and photosynthetic pigments (day 8). In conclusion, exposure to PAW increases the quality of Triticum aestivum L. cv. Glosa sprouts. Further optimization of PAW treatment is required for the most favorable impact on the accumulation of biomass and health-promoting compounds in sprouts.

Influence of Selenium Biofortification of Soybeans on Speciation and Transformation during Seed Germination and Sprouts Quality

Selenium (Se) biofortification during seed germination is important not only to meet nutritional demands but also to prevent Se-deficiency-related diseases by producing Se-enriched foods. In this study, we evaluated effects of Se biofortification of soybeans on the Se concentration, speciation, and species transformation as well as nutrients and bioactive compounds in sprouts during germination. Soybean (Glycine max L.) seedlings were cultivated in the dark in an incubator with controlled temperature and water conditions and harvested at different time points after soaking in Se solutions (0, 5, 10, 20, 40, and 60 mg/L). Five Se species and main nutrients in the sprouts were determined. The total Se content increased by 87.3 times, and a large portion of inorganic Se was transformed into organic Se during 24 h of germination, with 89.3% of the total Se was bound to soybean protein. Methylselenocysteine (MeSeCys) and selenomethionine (SeMet) were the dominant Se species, MeSeCys decreased during the germination, but SeMet had opposite trend. Se biofortification increased contents of total polyphenol and isoflavonoid compounds and amino acids (both total and essential), especially in low-concentration Se treatment. In conclusion, Se-enriched soybean sprouts have promising potential for Se supplementation and as functional foods.

Effect of stress on germination of djulis (Chenopodium formosanum Koidz.) sprouts: a natural alternative to enhance the betacyanin and phenolic compounds.

Germination is regarded as a natural method for improving the bioavailability of seed nutrients against stress, which enhances the accumulation of bioactive compounds. The present study aimed to determine the effect of stress (H2 O2 , catechin, gallic acid, tyrosine, and NaCl) during germination of djulis (Chenopodium formosanum Koidz.) sprouts on betacyanin, phytochemicals, and antioxidant capacities. The betacyanin and antioxidant activities of the djulis sprouts increased significantly compared to seeds. The lowest betacyanin was found in NaCl-stressed sprouts. The djulis sprouts reported the presence of celosianins I and II (50.72%), which was absent in seeds. Hydroxycinnamic acids accounted for > 60% of the total phenolic compounds in sprouts, whereas rutin predominated in the seeds. Germination under stress may represent an effective natural method for improving the bioactive potential of sprouts, an alternative to use seeds, in the development of bioactive compounds-enriched healthy foods that are good for public health. © 2022 Society of Chemical Industry.

UV and Visible Spectrum LED Lighting as Abiotic Elicitors of Bioactive Compounds in Sprouts, Microgreens, and Baby Leaves-A Comprehensive Review including Their Mode of Action.

Background: According to social demands, the agri-food industry must elaborate convenient safe and healthy foods rich in phytochemicals while minimising processing inputs like energy consumption. Young plants in their first stages of development represent great potential. Objective: This review summarises the latest scientific findings concerning the use of UV and visible spectrum LED lighting as green, sustainable, and low-cost technologies to improve the quality of sprouts, microgreens, and baby leaves to enhance their health-promoting compounds, focusing on their mode of action while reducing costs and energy. Results: These technologies applied during growing and/or after harvesting were able to improve physiological and morphological development of sprouted seeds while increasing their bioactive compound content without compromising safety and other quality attributes. The novelty is to summarise the main findings published in a comprehensive review, including the mode of action, and remarking on the possibility of its postharvest application where the literature is still scarce. Conclusions: Illumination with UV and/or different regions of the visible spectrum during growing and shelf life are good abiotic elicitors of the production of phytochemicals in young plants, mainly through the activation of specific photoreceptors and ROS production. However, we still need to understand the mechanistic responses and their dependence on the illumination conditions.

Strategies of Elicitation to Enhance Bioactive Compound Content in Edible Plant Sprouts: A Bibliometric Study.

Vegetable sprouts are a food source that presents high content of bioactive compounds which can also be enhanced through elicitation mechanisms. To better understand the scientific production and research trends on this topic, a bibliometric analysis by means of the Web of Science database was carried out. The results showed significant growth in research on the elicitation of edible plants sprouts. The three most productive journals were the Journal of Agricultural and Food Chemistry, followed by Food Chemistry and LWT-Food Science and Technology. The co-occurrence of keyword analysis of the different authors showed that the main research topics in this domain were ‘germination’, ‘antioxidant activity’, ‘sprouts’, ‘glucosinolates’ and ‘phenolics‘. The countries with the highest number of scientific publications were China, followed by India and USA. The productivity patterns of the authors conformed to Lotka’s law. This study provides an overview of research on elicitation to enrich bioactive compounds in sprouts, and the need to review and update the trends on this subject.

Constitutional and Induced Accumulation of Callose and Phenol Compounds as Elements of Systemic Resistance in Winter Wheat Sprouts

The amounts of accumulated callose and phenol compounds in sprouts of two winter wheat cultivars under inoculation with pathogenic fungus Pseudocercosporella herpotrichoides, the causing agent of wheat eyespot, were determined. Considerable differences were demonstrated in the constitutional and induced accumulation of phytoimmunity substances by the sprouts of the Myronivska 808 and Renan cultivars, which cause specific features of their resistance to pathogenic microorganisms.

Effect of Sprouting Temperature on Durum Wheat (Triticum Durum) Sprouts Nutritional Properties and Bioactive Compounds

Sprouting is an old food engineering tool to improve edible seeds nutritional value. It can improve carbohydrate digestibility and enhance levels of bioactive compounds. These changes are strongly related to sprouting conditions: temperature, light, duration. The aim of this research was to evaluate the effect of sprouting temperature on sprouts bioactive molecules and proximate composition of Chili Tunisian durum wheat (Triticum durum) seeds. Hence, two temperatures were tested: 18°C and 25°C. Analysis of ashes, proteins, lipids, reducing sugars, carotenoids, vitamin C, vitamin E, and total phenols contents and DPPH radical scavenging activity were done. Our results indicated that temperature influenced significantly proximate composition of sprouts, as well as bioactive compounds. Vitamin C and tocopherols levels were higher at 25°C, than in raw seeds. In contrast, a temperature of 18°C led to highest amounts of carotenoids and total phenols. In addition, antioxidant properties of durum wheat seeds were improved by sprouting only at 18°C. In conclusion, evolution of nutritional properties and bioactive compounds in sprouts were strongly dependent on sprouting temperature used.

Effect of chemical stress on germination of cv Dalia bean (Phaseolus vularis L.) as an alternative to increase antioxidant and nutraceutical compounds in sprouts

The aim of this study was to determine the effect of chitosan (CH), salicylic acid (SA) and hydrogen peroxide (H2O2) at different concentrations on the antinutritional and nutraceutical content, as well as the antioxidant capacity of bean sprouts (cv Dalia). All elicitors at medium and high concentrations reduced the antinutritional content of lectins (48%), trypsin inhibitor (57%), amylase inhibitor (49%) and phytic acid (56%). Sprouts treated with CH, SA and H2O2 (7μM; 1 and 2mM, and 30mM respectively) increased the content of phenolic compounds (1.8-fold), total flavonoids (3-fold), saponins (1.8-fold) and antioxidant capacity (37%). Furthermore, the UPLC-ESI-MS/MS analysis showed an increase of several nutraceutical compounds in bean sprouts treated with SA such as coumaric (8.5-fold), salicylic (115-fold), gallic (25-fold) and caffeic (1.7-fold) acids, as well as epigallocatechin (63-fold), rutin (41-fold) and quercetin (16.6-fold) flavonoids. The application of elicitors in bean seed during sprouting enhances their nutraceutical properties.

The influence of selenium addition during germination of Brassica seeds on health-promoting potential of sprouts

The correlation among selenium uptake, the content of bioactive compounds in sprouts, and biological activities triggered in cultured human cells by sprout extracts was investigated. Seeds of Brassica crops and rye were treated with SeO2 water solution. The selenium levels in sprouts increased from 1.0–4.1 to 53.3–382 μg/g dw with no influence on plant physiology according to the indices used. Neither the composition of glucosinolates (GL) in Brassica sprouts nor the myrosinase activity nor the composition of GL breakdown lipophilic products were significantly affected. In all Brassica sprouts, conversion to health-promoting isothiocyanates (ITC) and indoles corresponded to only 1% of total GLs. Low ITC concentration may explain observed lack of induction of glutathione S-transferases (GST) and quinone oxidoreductase (NQO) detoxifying enzymes in HT29 cells exposed to sprout extracts. The insignificant impact on cell growth and genome function suggests that Brassica sprouts may be safe vehicle of selenium to combat its dietary deficiency.

Improving the phytochemical composition of broccoli sprouts by elicitation

In order to increase the concentrations of bioactive compounds in broccoli sprouts, exogenous elicitors: methionine, tryptophan, chitosan, salicylic acid (SA) and methyl jasmonate (MeJA) were sprayed at different concentrations during germination. The effects of the elicitors on vitamin C, phenolic compounds and glucosinolate levels were assessed in 3, 5 and 7-days old seedlings. The application of 200, 300 μM SA and 0.01% chitosan induced increases, by 26%, 18% and 54%, respectively in the content of vitamin C in 5 days old broccoli sprouts. Flavonoid concentration was also increased by 31% and 33% after 10 μM MeJA and 100 μM SA treatments, respectively in 7 days old broccoli sprouts. In contrast, methionine or tryptophan solutions did not positively affect the vitamin C or the phenolic compounds in the sprouts. The individual classes of glucosinolates respond differently to the elicitor treatment. Methionine, a sulphur-containing amino acid, applied at 5 mM, significantly increased, by 32%, the levels of aliphatic glucosinolates. However, indole glucosinolates presented a significant response to the induction with tryptophan, SA or MeJA treatments. Tryptophan at 10 mM concentration augmented by 1.8-fold the indole glucosinolate concentration in 7 days old broccoli sprouts, and the treatments of 100 μM SA and 25 μM MeJA also induced significantly higher amounts of indole glucosinolates (by 33% and 51%, respectively) in 7 days old treated broccoli sprouts. Therefore, the application of elicitors could be a useful tool for improving the bioactive metabolites in broccoli sprouts for fresh consumption or functional ingredients for nutraceuticals foods.

Nutritional and health-related compounds in sprouts and seeds of soybean (Glycine max), wheat (Triticum aestivum.L) and alfalfa (Medicago sativa) treated by a new drying method

Nutritional and health-related compounds of alfalfa, wheat and soybean seeds dried by new drying process called the DIC process (controlled instantaneous pressure release) were evaluated, before and after sprouting. Vitamins (A, B1, B2, B6, C and E), minerals (Fe, Mg, Ca, K, Mn, Na, Cu and Zn) and phytoestrogens (genistein and daidzein ) content were determined. Alfalfa, soybean and wheat seeds dried by DIC showed similar content of biological compounds to seeds dried by traditional processes. Sprouting DIC-seeds significantly increased the levels of vitamins, minerals and phytoestrogens, improving their nutrititional value and health quality compared to fresh products. The increase of 1250-fold and 10-fold of the initial vitamin A and vitamin C content, respectively, of alfalfa seeds due to sprouting is remarkable. Sprouts from DIC-seeds showed a significantly higher vitamin A content than sprouts obtained from seeds dried by other methods. Soybean sprouts obtained from DIC-seeds showed a significant increase in free phytoestrogens, quantified as genistein (70.34±5.8 mg kg–1 d.w.) and daidzein (109.17.3±17.3 mg kg–1 d.w.).