Changes In Composition Research Articles

Lithogeochemistry of upper precambrian terrigenous rocks of Belarus. Communication 1. Bulk chemical composition, general features and anomalies

The first of two publications is devoted to the lithogeochemical features study of the pilot collection of the Upper Precambrian sandstones and siltstones samples, selected from four boreholes: Bogushevskaya 1, Bykhovskaya, Lepel 1 and Korminskaya (Belarus). This article analyzes the general features of their bulk chemical composition and shows the possibilities and limitations for further reconstructions. It has been established that the Riphean and the Vendian rocks included in the pilot collection, visually identified as sandstones, are actually quartz, feldspathic-quartz and arkose varieties with different cement types. The Vendian “siltstones” in their geochemical characteristics correspond to coarse- and fine-grained siltstones and, to a greater extent, mudstones with a predominance of illite, as well as various admixtures of berthierine, kaolinite and smectite. Considering the trace elements enrichment factors of these rocks a number of dissimilarities were identified, caused both by the source rocks composition and sedimentary environments changes. The data point position of the studied samples on the Zr/Sc–Th/Sc diagram suggests that the composition of all the Riphean and the Vendian rocks is dominated by first sedimentation cycle material. This suggests that the lithogeochemical characteristics of the rocks of the pilot collection quite correctly reflect similar features of the source rock complexes and can be used to reconstruct the paleogeodynamic and paleoclimatic factors that controlled the accumulation of the Riphean and the Vendian sedimentary sequences of Belarus.

Age of Stratospheric Air: Progress on Processes, Observations, and Long‐Term Trends

AbstractAge of stratospheric air is a well established metric for the stratospheric transport circulation. Rooted in a robust theoretical framework, this approach offers the benefit of being deducible from observations of trace gases. Given potential climate‐induced changes, observational constraints on stratospheric circulation are crucial. In the past two decades, scientific progress has been made in three main areas: (a) Enhanced process understanding and the development of process diagnostics led to better quantification of individual transport processes from observations and to a better understanding of model deficits. (b) The global age of air climatology is now well constrained by observations thanks to improved quality and quantity of data, including global satellite data, and through improved and consistent age calculation methods. (c) It is well established and understood that global models predict a decrease in age, that is, an accelerating stratospheric circulation, in response to forcing by greenhouse gases and ozone depleting substances. Observational records now confirm long‐term forced trends in mean age in the lower stratosphere. However, in the mid‐stratosphere, uncertainties in observational records are too large to confirm or disprove the model predictions. Continuous monitoring of stratospheric trace gases and further improved methods to derive age from those tracers will be crucial to better constrain variability and long‐term trends from observations. Future work on mean age as a metric for stratospheric transport will be important due to its potential to enhance the understanding of stratospheric composition changes, address climate model biases, and assess the impacts of proposed climate geoengineering methods.

Immediate and prolonged effects of hydrogen peroxide to a natural plankton community from a tropical reservoir: ecotoxicological aspects and compositional changes

Immediate and prolonged effects of hydrogen peroxide to a natural plankton community from a tropical reservoir: ecotoxicological aspects and compositional changes

Thermal cycling performance of GYbZ/YSZ thermal barrier coatings with different microstructures based on finite element simulation

Three (Gd0.9Yb0.1)2Zr2O7/YSZ double ceramic layer (DCL) thermal barrier coatings (TBCs) with different microstructures were manufactured by electron-beam physical vapor deposition (EB-PVD). The thermal cycling behavior of the three TBCs was evaluated comparatively at 1150 ℃. The results showed that TBCs with small columnar grains exhibited the best thermal cycling performance among all the tested TBCs. The effect of microstructure on thermal cycling behavior, including crack evolution and failure mode was discussed. A finite element model tailored to the microstructural characteristics of the DCL coatings was established. Several in-plane stress concentration points appeared in the taller GYbZ column due to discontinuities in strain tolerance resulting from sudden structural or compositional changes. Analysis of the strain energy release rate variation for each concentration point suggested that the predicted delamination position and process aligned with experimental results.

Coupling Effect of Elemental Carbon and Organic Carbon on the Changes of Optical Properties and Oxidative Potential of Soot Particles under Visible Light.

Soot particles, coming from the incomplete combustion of fossil or biomass fuels, feature a core-shell structure with inner elemental carbon (EC) and outer organic carbon (OC). Both EC and OC are known to be photoactive under solar radiation. However, research on their coupling effect during photochemical aging remains limited. This study examines how the optical properties and oxidative potential (OP) of wood, coal, and diesel soot particles with varying EC and OC levels are affected by exposure to visible light. Wood soot, which has the highest OC content, showed the most significant changes in both optical properties and OP, indicating its highest sensitivity to visible light aging. Molecular composition analysis revealed that the reduction of polycyclic aromatic hydrocarbons (PAHs) and methyl-PAHs primarily affects the optical properties, while oxygenated PAHs play a major role in OP. Combined with the results from reactive oxygen species detection, it is suggested that EC initiates photoreactions by generating superoxide anions, while OC undergoes compositional changes that result in subsequent atmospheric effects. These findings enhance our understanding of the photochemical aging process of soot particles and their implications for climate and health.

Association between changes in body composition and progression of liver fibrosis in patients with type 2 diabetes mellitus.

The correlation between type 2 diabetes mellitus (T2DM) and the occurrence of liver fibrosis is well-established. However, the longitudinal association between body composition and liver fibrosis progression in patients with T2DM remains incompletely explored. Total of 390 patients with T2DM underwent body composition assessments, followed by a median duration of 2.13 years. The calculated parameters included body mass index (BMI), fat mass index (FMI), trunk fat mass index (TFMI), appendicular skeletal muscle mass index (ASMI), muscle/fat mass ratio (M/F) and appendicular skeletal muscle mass/trunk fat mass ratio (A/T). Liver fibrosis was evaluated through liver stiffness measurement (LSM). Patients were classified according to BMI and body composition, followed by a comprehensive investigation into the impact of body composition changes on liver fibrosis outcomes. Among 72 patients with incident advanced liver fibrosis at readmission, ΔBMI, ΔFMI and ΔTFMI increased, while ΔM/F and ΔA/T decreased. Individuals who kept obese had a dramatically elevated hazard of incident advanced liver fibrosis compared to those who kept non-obese, with an adjusted odds ratio of 3.464. When TFMI heightened, the hazard of incident advanced liver fibrosis was 3.601 times higher compared to the decreased group. Additionally, individuals in increased ASMI and A/T groups showed a slight advantage in preventing incident advanced liver fibrosis compared to the stable groups. Stable obesity was associated with a greater hazard of liver fibrosis advancement, and an increase in TFMI may promote the progression of liver fibrosis. Maintaining a balanced muscle/fat ratio appeared to help prevent the progression.

Study on the Applicability of Deterioration Detection Techniques for Sandstone Heritage in Multi Environment using MLP-Attention Model

Abstract. Stone cultural heritage, encompassing a broad spectrum of artifacts such as stone artworks, buildings, tools, and utensils, represents one of the most significant categories of cultural heritage. However, the conservation of these cultural heritage faces challenges from the process of deterioration. This degradation not only compromises the structural integrity of the heritage but also results in the loss of invaluable historical information. Thus, there emerges a critical demand for effective methods to detect and assess the condition of stone cultural heritage, enabling timely and precise conservation interventions. Here we obtained sandstone samples under different deteriorative environments through laboratory-simulated deterioration experiments and employed a variety of detection technologies to capture a series of changes in the deterioration detection parameters during the sandstone deterioration process. Subsequently, a deep learning model was established to correlate the detection parameters with the degree of stone deterioration. A SHAP analysis was then conducted to determine the contribution of various parameters to the degree of stone weathering under different experimental environments, providing recommendations for selecting appropriate detection technologies and indicators adapted to different deterioration environments. To further analyze the deterioration processes of the stone samples, XRD analysis was conducted to observe changes in mineral composition throughout the deterioration process. SEM images were utilized to examine the changes in micro-morphology and the internal pore structure associated with deterioration. This study provides a basis for the scientific design of deterioration detection schemes by selecting the most suitable testing technology for optimal deterioration assessment under specific environmental conditions.

The effects of Carpinus betulus expansion on soil properties under changing climatic conditions

Carpinus betulus (CB) is becoming increasingly important in the forests of Central Europe and is significantly increasing its proportion in various habitat types. We have analysed how the increase in CB affects soil properties. The study was conducted in the Białowieża Forest (BF). We monitored the changes in CB cover between 1962 and 2013 on 56 permanent plots and collected samples of the litter sub-horizons and the content of the underlying A horizons for laboratory analyses in 2014. During the study period, there was a significant increase in CB cover in the second tree layer by an average of 17.4 % (±1.8) and in the shrub layer by 3.8 % (±1.0), as well as an increase in species diversity from 41 % in the past to 59 % currently. The C/N ratio of the Oi sub-horizon was 32.0 (±0.9), and NH4+ compounds predominated over NO3-, with all nitrogen forms most closely associated with the C/N ratio. In the Oea sub-horizon, the C/N ratio was 23.4 (±0.4), and the sum of mineralised nitrogen reached 8%, with a clear predominance of NO3-. The characteristic most strongly associated with the increase in CB coverage was the NO3- content in the Oea sub-horizon. The MID analysis confirmed that the change in CB cover was not only significant, but also ecologically important. We suggest that there is a feedback loop in which the generally observed climate changes lead to trees occupying new habitats that supply the forest floor with high quality litter, which in turn affects the soil and promotes the persistence of changes in the species composition of the forest.

Characterizing body composition modifying effects of a glucagon-like peptide 1 receptor-based agonist: A meta-analysis.

Diabetes is an independent risk factor for muscle mass loss, with possible mechanisms including impaired insulin signalling and chronic inflammation. The use of a glucagon-like peptide 1 (GLP-1) receptor-based agonist could lead to weight reduction, which might result from the loss of both fat and skeletal muscle. However, the body composition-modifying effects of GLP-1 receptor-based agonists have not been systematically characterized. PubMed, EMBASE, the Cochrane Center Register of Controlled Trials for Studies and Clinicaltrial.gov were searched from inception to October 2023. Randomized controlled trials of GLP-1 receptor agonist or glucose-dependent insulinotropic polypeptide/GLP-1 receptor dual agonist, which reported the changes of body composition, were included. The results were computed as weighted mean differences (WMDs) and 95% confidence intervals (CIs) in a random-effects model. In all, 19 randomized controlled trials were included. When compared with controls, substantial reductions in fat body mass were observed in patients using GLP-1 receptor-based agonist treatment (WMD = -2.25 kg, 95% CI -3.40 to -1.10 kg), with decrease in areas of both subcutaneous fat (WMD = -38.35 cm2, 95% CI, -54.75 to -21.95 cm2) and visceral fat (WMD = -14.61 cm2, 95% CI, -23.77 to -5.44 cm2). Moreover, greater reductions in lean body mass were also observed in GLP-1 receptor-based agonist users compared with non-users (WMD = -1.02 kg, 95% CI, -1.46 to -0.57 kg), while the changes in lean mass percentage were comparable between GLP-1 receptor-based agonist users and non-users. Compared with the controls, GLP-1 receptor-based agonist users experienced greater reductions in fat body mass, with body shaping effects in terms of both subcutaneous fat mass and visceral fat mass. Although greater reductions in lean body mass were also observed in GLP-1 receptor-based agonist users, the changes in lean mass percentage were comparable between the users and non-users.

Examination of duodenal and colonic microbiome changes in mouse models of acute and chronic pancreatitis

The exocrine pancreas is the main source of digestive enzymes which are released from secretory vesicles of acinar cells into the small intestine. Enzymes, including amylases, proteases and lipases, degrade the ingested food and thus determine the nutritional substrate for the gut microbiota. Acute (AP) and chronic pancreatitis (CP) are associated with a transitional or progressive exocrine pancreatic dysfunction, we analysed in the present study how an experimental induction of pancreatitis in mouse models affects the colonic and duodenal microbiome composition. Evaluation by 16 S rRNA gene sequencing revealed specific microbiome changes in colonic as well as in duodenal samples in different models of AP and CP. Mild acute pancreatitis, which is associated with a transient impairment of pancreatic secretion showed only minor changes in microbial composition, comparable to the ones seen in progressive dysfunctional mouse models of CP. The strongest changes were observed in a mouse model of severe AP, which suggest a direct effect of the immune response on gut microbiome in addition to a pancreatic dysfunction. Our data indicate that highly dysbiotic microbiome changes during pancreatitis are more associated with the inflammatory reaction than with a disturbed pancreatic secretion.

The Consumption of Lacticaseibacillus rhamnosus HDB1258 Changes Human Gut Microbiota and Induces Immune Enhancement Through NK Cell Activation.

The gut microbiota can play an important role in enhancing the host's complex immune system. In this regard, many studies indicate that probiotics consumption has a beneficial impact on alterations in the composition of the gut microbiota. Our previous study demonstrated that the oral administration of Lacticaseibacillus rhamnosus HDB1258 (HDB1258) enhances immune cell activity and alters the composition of gut microbiota in C57BL/6 mice, thereby showing its potential as a novel immunostimulatory ingredient. Therefore, this clinical trial assessed the effects of HDB1258 on human natural killer (NK) cell activity and changes in gut microbiota. It also investigated the correlation between gut microbiota and NK cell activity following HDB1258 supplementation. Participants (n = 71) were randomized into placebo and HDB1258 groups, and NK cell activity and gut microbiota were investigated at baseline (week 0) and endline (week 8). The present study showed that HDB1258 significantly increased NK cell activity and resulted in positive regulatory effects on the gut microbial balance in subjects compared to the placebo group. HDB1258 affected the gut microbial balance by inducing the growth of beneficial bacteria such as Lactococcus and Sutterella. Especially, the changes in Escherichia-Shigella composition were negatively correlated with the changes in NK cell activity after HDB1258 consumption. There was also a positive correlation between the NK cell activity in the HDB1258 group and the composition of Prevotella 9 and Adlercreutzia. These findings suggest that HDB1258 may improve the host's intestinal environment by regulating gut bacteria related to immune response and promote NK cell activation. This study was registered at clinical research information service (CRIS: KCT0008204).

Changes in Microbial Ecosystems and Serum Metabolomics by Diet Supplementation With Enramycin in Weaning Piglets.

Antibiotics are used in swine production for growth promotion and disease prevention, raising concerns about environmental contamination and antibiotic resistance. In this study, we investigated the effects of enramycin supplementation on piglet growth, gut microbiota and blood metabolites. Enramycin promotes piglet growth and temporarily reduces diarrhoea. Gut microbiota analysis revealed changes in microbial composition, including an increase in the abundance of Limosilactobacillus reuteri. Metabolomic analysis has identified elevated levels of dimethylglycine, a known growth-promoting factor, in the enramycin group. Liver gene expression analysis revealed increased mRNA levels of ALDH and dimethylglycine dehydrogenase, which are enzymes involved in dimethylglycine metabolism. The enramycin-treated group had a higher concentration of acetic acid in caecal contents, and their caecal acetic acid concentrations were positively correlated with the abundance of L. reuteri and the content of serum dimethylglycine, respectively. These findings suggest that the promotion effect of enramycin on piglet growth is related to the gut microbiota, blood metabolites and liver gene expression, which provide insights into antibiotic alternatives for swine production.

Morphine-induced hyperalgesia impacts small extracellular vesicle miRNA composition and function.

Morphine and other synthetic opioids are widely prescribed to treat pain. Prolonged morphine exposure can paradoxically enhance pain sensitivity in humans and nociceptive behavior in rodents. To better understand the molecular mechanisms underlying opioid-induced hyperalgesia, we investigated changes in miRNA composition of small extracellular vesicles (sEVs) from the serum of mice after a morphine treatment paradigm that induces hyperalgesia. We observed significant differential expression of 18 miRNAs in sEVs from morphine-treated mice of both sexes compared to controls. Several of these miRNAs were bioinformatically predicted to regulate cyclic AMP response element binding protein (CREB), a well-characterized transcription factor implicated in pain and drug addiction. We confirmed the binding and repression of Creb mRNA by miR-155 and miR-10a. We tested if serum-derived sEVs from morphine-treated mice could elicit nociceptive behavior in naïve recipient mice. Intrathecal injection of 1 μg sEVs did not significantly impact basal mechanical and thermal threshold in naïve recipient mice. However, prophylactic 1 μg sEV administration in recipient mice resulted in faster resolution of complete Freund's adjuvant-induced mechanical and thermal inflammatory hypersensitivity. Other behaviors assayed following administration of these sEVs were not impacted including sEV conditioned place preference and locomotor sensitization. These results indicate that morphine regulation of serum sEV composition can contribute to analgesia and suggest a potential for sEVs to be a non-opioid therapeutic intervention strategy to treat pain.

Biotic and abiotic properties mediating sediment microbial diversity and function in a river-lake continuum.

A river-lake system plays an important role in water management by providing long-term and frequent water diversions. However, hydrological connectivity in the system can have a profound effect on sediment microbial communities through pH, nutrient concentrations, and benthos invertebrates. Consequently, identifying the key environmental factors and their driving mechanisms is vital for microbial adaptation strategies to extreme environments. In this study, we analyzed the significant difference in sediment bacterial and fungal community structures and diversity indices among Dongting Lake and its tributary rivers, which worked as a typical river-connected lake ecosystem. There were significant differences in biotic and abiotic environments in the sediment habitats of Dongting Lake and its tributary rivers. Random forest analysis revealed that pH and Mollusca were found to be the most important abiotic and biotic variables for predicting both bacterial and fungal community structures, respectively. The beta diversity decomposition analyses showed that the bacterial and fungal community compositional dissimilarities among different sections were dominated by species replacement processes, with more than half of the OTUs in each section being unique. Notably, both biotic and abiotic factors affected the number and the relative abundance of these bacterial and fungal unique OTUs, leading to changes in community composition. Mollusca, pH, TP, NO3-N, and NH4-N were negatively related to the relative abundance of Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes, and Ascomycota, while Annelida and ORP were positively related to the relative abundance of Actinobacteria and Gemmatimonadetes. Additionally, PICRUSt analysis revealed that the functional dissimilarity among lakes and rivers was strengthened in unique species compared to all species in bacterial and fungal communities, and the changes of functional types helped to improve the habitat environment in the main Dongting Lake and promote the process of microbial growth. From our results, the role of macrozoobenthos and physicochemical characteristics in driving the sediment microbial community spatial variations became clear, which contributed to further understanding of the river-lake ecosystem.

Efficacy of Sodium Fluoride and Fluoridated Calcium Phosphate in Mitigating Dental Erosion on Human Enamel: An In Vitro Analysis.

With increasing prevalence of dental erosion, this study explores the protective role of traditional fluoride-based products and newer formulations on eroded enamel. To assess the protective effectiveness of casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) and sodium fluoride (NaF) on human enamel against erosion using surface microhardness (SMH) and Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analyses. Ten extracted human third molars were sectioned to obtain 40 enamel sections and randomly assigned into four groups (n = 10) and treated as follows: G1 (Sound Enamel), G2 (Erosive Challenge), G3 (CPP-ACPF + Erosive Challenge), and G4 (NaF + Erosive Challenge). All samples were subjected to Vicker's SMH analysis, while changes in surface morphology and elemental composition were validated in few representative samples using FTIR and SEM, respectively. Paired samples test, Kruskal-Wallis test, and Tukey HSD test were performed using SPSS software version 23 setting P value < 0.05 as statistically significant. The mean SMH1 values for the experimental groups G3 and G4 were significantly higher (426.58VHN and 455.83VHN) when compared to G1 (P = 0.000) and G2 (P = 0.000). In SEM analysis, G2 showed eroded honeycomb appearance compared to the smooth homogenous surface of G1, while both G3 and G4 showed deposition of some precipitates. FTIR analysis revealed that in G3 and G4, a characteristic peak of phosphate vibrations between 528 and 823 cm-1 and carbonate bands at 845-932 cm-1 was observed. Both CPP-ACPF and NaF demonstrated a protective effect on enamel against erosive challenge by an orange juice-based beverage.

Anthropometric Profile and Position-Specific Changes in Segmental Body Composition of Professional Football Players Throughout a Training Period.

Body and anthropometric profiles of football players vary depending on the physiological and technical demands imposed by different positions. The aim of this study was to evaluate the body composition of professional soccer players in relation to their position on the field during a training macrocycle. The Direct Segmental Multi-Frequency Bioelectrical Impedance Analysis method was used to analyze 58 players at six key moments of the macrocycle. The results show that body profiles are adjusted to the specific demands of each position. Midfielders showed the lowest muscle mass, while defenders showed many notable changes. In general, as the season progressed, all field players experienced an increase in trunk body fat. Fat and lean mass values of goalkeepers differed greatly from the rest. The greatest variations in body composition were observed during pre-season and transition in relation to variations in training load and competitive intensity. The results suggest that the phase of the macrocycle has a greater influence on these variations, although the physical characteristics of each position are relevant. Understanding these dynamics allows for the design of more personalized and efficient training programs to optimize the performance of footballers according to their roles and each stage of the season.

Data-independent acquisition proteomic analysis of the brain microvasculature in Alzheimer’s disease identifies major pathways of dysfunction and upregulation of cytoprotective responses

Brain microvascular dysfunction is an important feature of Alzheimer’s disease (AD). To better understand the brain microvascular molecular signatures of AD, we processed and analyzed isolated human brain microvessels by data-independent acquisition liquid chromatography with tandem mass spectrometry (DIA LC–MS/MS) to generate a quantitative dataset at the peptide and protein level. Brain microvessels were isolated from parietal cortex grey matter using protocols that preserve viability for downstream functional studies. Our cohort included 23 subjects with clinical and neuropathologic concordance for Alzheimer’s disease, and 21 age-matched controls. In our analysis, we identified 168 proteins whose abundance was significantly increased, and no proteins that were significantly decreased in AD. The most highly increased proteins included amyloid beta, tau, midkine, SPARC related modular calcium binding 1 (SMOC1), and fatty acid binding protein 7 (FABP7). Additionally, Gene Ontology (GO) enrichment analysis identified the enrichment of increased proteins involved in cellular detoxification and antioxidative responses. A systematic evaluation of protein functions using the UniProt database identified groupings into common functional themes including the regulation of cellular proliferation, cellular differentiation and survival, inflammation, extracellular matrix, cell stress responses, metabolism, coagulation and heme breakdown, protein degradation, cytoskeleton, subcellular trafficking, cell motility, and cell signaling. This suggests that AD brain microvessels exist in a stressed state of increased energy demand, and mount a compensatory response to ongoing oxidative and cellular damage that is associated with AD. We also used public RNAseq databases to identify cell-type enriched genes that were detected at the protein level and found no changes in abundance of these proteins between control and AD groups, indicating that changes in cellular composition of the isolated microvessels were minimal between AD and no-AD groups. Using public data, we additionally found that under half of the proteins that were significantly increased in AD microvessels had concordant changes in brain microvascular mRNA, implying substantial discordance between gene and protein levels. Together, our results offer novel insights into the molecular underpinnings of brain microvascular dysfunction in AD.

Effect of the Mediterranean diet on the fecal long-chain fatty acid composition and intestinal barrier integrity: An exploratory analysis of the randomized controlled LIBRE trial.

We recently showed that adherence to the Mediterranean diet increased the proportion of plasma omega-3 polyunsaturated fatty acids (n-3 PUFAs), which was associated with an improved intestinal barrier integrity. In the present exploratory analysis, we assessed fecal fatty acids in the same cohort, aiming to investigate possible associations with intestinal barrier integrity. Women from the LIBRE randomized controlled trial, characterized by an impaired intestinal barrier integrity, followed either a Mediterranean diet (intervention group, IG, n=33) or a standard diet (control group, CG, n=35). At baseline (BL), month 3 (V1), and month 12 (V2), plasma lipopolysaccharide binding protein (LBP), fecal zonulin, and fecal fatty acids were measured. In the IG, fecal proportions of palmitoleic acid (16:1, n-7) and arachidonic acid (20:4, n-6) decreased, while the proportion of linoleic acid (18:2, n-6) and alpha linoleic acid (18:3, n-3) increased (BL-V1 and BL-V2, all P<0.08). In the CG, fecal proportions of palmitic acid and arachidic acid increased while the proportion of linoleic acid decreased (BL-V1, all P<0.05). The decrease in the proportion of palmitoleic acid correlated with the decrease in plasma LBP (∆V1-BL r=0.72, P<0.001; ∆V2-BL r=0.39, P<0.05) and correlated inversely with adherence to the Mediterranean diet (Mediterranean diet score; ∆V1-BL r=-0.42, P=0.03; ∆V2-BL r=-0.53, P=0.005) in the IG. Our data show that adherence to the Mediterranean diet induces distinct changes in the fecal fatty acid composition. Furthermore, our data indicate that the fecal proportion of palmitoleic acid, but not fecal n-3 PUFAs, are associated with intestinal barrier integrity in the IG.

Macrophyte litter mixtures mediate decomposition processes in coastal sediments

Understanding litter decomposition processes in coastal macrophyte habitats is critically important for predicting ecosystem functioning. However, decomposition processes of litter mixtures in coastal habitats remain largely unexplored. Here, we evaluated the litter mixture effects on the decomposition of six marine macrophytes (two seagrasses and four macroalgae) through in situ litter-mixing experiments with five levels of litter species richness and 36 different litter compositions. We found that the litter species identity and composition, rather than species richness, were crucial in structuring benthic faunal communities. Macroalgal litter, particularly Sargassum sp., hosted higher numbers of polychaetes and crustaceans than seagrass litter. More macroalgal presence induced faster decomposition rates of seagrass litter in the late stage, but not in the early stage. These findings suggest that changes in macrophyte diversity and composition can alter decomposition processes and, consequently, the sediment organic carbon stock through the transition of litter sources and benthic faunas.

Impact of periodontal therapy on oral bacterial composition in individuals diagnosed with advanced periodontal disease.

Introduction. Negative changes in the microbial composition have been extensively studied in individuals with periodontal disease.Gap Statement. The changes in the oral microbiota after treating this disease are still unknown.Aim. We sought to elucidate the distinctive traits of salivary microbiota in individuals displaying healthy gums and those with severe periodontitis (SP) and examine the influence of periodontal therapy.Methodology. Periodontal pocket depths were examined to determine disease severity. The presence and quantity of oral Helicobacter pylori (associated with periodontal disease) were determined. Sequencing of 16S ribosomal DNA and bioinformatic analyses were performed to assess oral bacterial compositions in patients.Results. Sequencing analysis of 16S ribosomal DNA revealed a significant reduction in the abundance of operational taxonomic units (OTUs) and Chao1 and Abundance coverage-based estimator(ACE) indices in the oral cavities of individuals with SP compared to those of the healthy controls. However, these parameters showed significant recovery after appropriate treatment severe periodontitis after treatment (TSP). Additionally, the levels of harmful Bacillales and Spirochetes significantly increased, whereas the presence of beneficial Euryarchaeota significantly decreased in the SP group. The TSP group exhibited considerably augmented abundances of Burkholderiaceae and Veillonella, while noteworthy reductions in the pathogenic microbiota (Clostridia, Fusobacteria and Spirochaetes) were noticed compared to those of the SP group. Functionally, these modified OTUs were extensively implicated in 41 metabolic pathways.Conclusion. Our study demonstrates that nonsurgical periodontal therapy can effectively reduce the diversity of the oral microbiota, thereby potentially enhancing the treatment efficacy in patients with periodontal disease.