The aim: study the effect of the synthetic anabolic steroid 19-nortestosterone on the fractional composition of blood plasma proteins, as well as the total protein and albumin content, indicators of the metabolism of carbohydrates, lipids, macro- and microelements in the fish hepatopancreas. Materials and methods. The experiments were conducted on two-year-old carp (Cyprinus carpio L.), which were kept for 24 hours in aquariums with a volume of 40 dm3 with different concentrations of 19-nortestosterone in water at optimal temperature, oxygen content, and pH. Spectrophotometric methods and an automatic biochemical analyzer were used to determine metabolic indicators in fish, and disk electrophoresis in PAGE was used to determine the fractional composition of proteins. Results. It was established that the steroid hormone 19-nortestosterone, the concentration of which in the water of the fish of the experimental groups was 50 and 200 μg/dm3, respectively, increased the level of total protein and albumin, the concentration of glucose, triglycerides, creatinine, inorganic phosphorus and iron in the hepatopancreas and did not affect the calcium content . In the blood plasma of carp, kept in water with a concentration of 19-nortestosterone of 50 μg/dm3, the content of proteins with a molecular weight of 240-231 increases; 215-179; 169-146 and 105-95 kDa, decreases - 35-33 kDa, fractions of proteins with a molecular weight of 228-220 appear; 116-105 and 48-44 kDa. An increase in the concentration of 19-nortestosterone in water to 200 μg/dm3 increased the content of proteins with a molecular weight of 240-231 and 215-179 kDa in the blood plasma of fish, and decreased it by 35-33 kDa, causing the redistribution of individual protein fractions due to the appearance of proteins with a molecular weight of 240 -228; 116-105 and 48-44 kDa in the absence of proteins 42-41; 38-36 and 24-20 kDa. Conclusions. Based on the obtained results, it was concluded that the anabolic synthetic steroid 19-nortestosterone stimulates the biosynthesis of proteins, the metabolism of carbohydrates and lipids, and its effect on the content of minerals in the hepatopancreas and the fractional composition of blood plasma proteins in carp fish was proven
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