Shale oil reservoirs usually have various lithofacies with complex spatial patterns. Seismic reflections usually reveal the combination of sets of thin mud shales with various lithofacies. The seismic response characteristics of the lithofacies and the differences in seismic response of different lithofacies are still unclear, making the reservoir description difficult. The complex tectonic structures, diverse lithofacies, thin interlayers, fractures with various length scales, and various fracture development zones in the shale oil reservoir have placed very high demands on the design and construction method during the physical modeling of the shale oil reservoir. Facing these challenges, a physical modeling study on shale oil reservoirs with complex lithofacies and meter-scale structures is conducted. The target is the shale oil reservoir of the [Formula: see text] subsegment in the Jiyang depression. The complex physical model of a shale oil reservoir, with the thin stratigraphic layers and the complicated lithofacies and fracture patterns to date, has been designed and constructed in a laboratory. In total, 25 combinations of materials have been developed to simulate various lithofacies with different elastic parameters, and the error of the wave velocity simulation is less than 2.35%. The simulation of 1 m thick interlayers and the construction method for seismic physical models with meter-scale geologic structures has been realized. The quality control analysis finds that the simulation accuracy of small-scale geologic structure, complex lithofacies, and fractures is relatively reliable. Based on the physical model of the shale oil reservoir, high-density and wide-azimuth seismic data are acquired. Preliminary data processing and analysis find that the model layers have clear reflection characteristics, providing reliable data for the next step of seismic characteristics analysis of complex lithofacies shale oil reservoirs. The methodology has improved the capacity of seismic physical modeling to simulate complex oil and gas reservoirs, enabling the seismic physical simulation method to be applied in analyzing the commonly observed complex lithofacies, fractures, and meter-scale structures in unconventional oil and gas reservoirs.