Compatible Protocol Research Articles

A forward compatible IoT protocol and framework addressing concerns due to internet-outage

Internet of things (IoT) paradigm is becoming ubiquitous in everyday life. IoT-based systems are increasingly getting deployed in generic household, healthcare, public utilities, etc., related application. The reason for such rapid acceptance is in majority due to the internet's feature of connect from anywhere. However, due to some weaknesses inherent with the internet itself, IoT deployments also face certain challenges. Though there is a growing number of IoT-related reference architectures, frameworks, guidelines, platforms and standards, majority of them are yet to address the concerns due to internet outage. Furthermore, such growing numbers of architectures, frameworks, etc., also means bountiful amounts of both opportunities and risks for IoT vendors and original equipment manufacturers (OEMs). Of course, the IoT-device consumers also run the risks if the purchased products frameworks incompatible. We present One IoT version 2 (v2), an IoT framework and protocol that addresses the internet outage scenario. Similar to version 1 (v1), One IoT v2 is also forward compatible.

LEO Satellite Constellation for Internet of Things

Internet of Things (IoT) is one of the evolutionary directions of the Internet. This paper focuses on the low earth orbit (LEO) satellite constellation-based IoT services for their irreplaceable functions. In many cases, IoT devices are distributed in remote areas (e.g., desert, ocean, and forest) in some special applications, they are placed in some extreme topography, where are unable to have direct terrestrial network accesses and can only be covered by satellite. Comparing with the traditional geostationary earth orbit (GEO) systems, LEO satellite constellation has the advantages of low propagation delay, small propagation loss and global coverage. Furthermore, revision of existing IoT protocol are necessary to enhance the compatibility of the LEO satellite constellation-based IoT with terrestrial IoT systems. In this paper, we provide an overview of the architecture of the LEO satellite constellation-based IoT including the following topics: LEO satellite constellation structure, efficient spectrum allocation, heterogeneous networks compatibility, and access and routing protocols.

A forward compatible IoT protocol and framework addressing concerns due to internet-outage

A forward compatible IoT protocol and framework addressing concerns due to internet-outage

안드로이드 앱을 이용한 실시간 유료 방송 취약점 분석

동영상 실시간 스트리밍 (Live Streaming)이란 텔레비전 생방송처럼 촬영한 정보를 실시간으로 사용자의 동영상 플레이어로 보내 재생하도록 하는 방식을 말한다. 비디오와 오디오를 실시간으로 인코딩하여 많은 사용자들이 동시에 시청하기 위해서는 RTMP (Real Time Messaging Protocol), HLS (HTTP Live Streaming) 등 실시간 스트리밍을 지원하는 프로토콜이 필요하다. 본 논문에서는 실시간 스트리밍을 제공하는 국내 6개 OTT (Over the Top) 업체의 어플리케이션을 대상으로 패킷 캡처를 통해 어플리케이션들의 패킷을 분석하였다. 채널 목록을 암호화하지 않거나, 유료채널에 적합하지 않은 프로토콜을 사용함으로써 유료채널을 무료로 시청할 수 있다는 취약점을 밝히고자 한다. Live streaming is a method to provide media service by sending recoded media to a user's video player. In order to provide video and audio contents in real-time for a large number of users simultaneously, live streaming compatible protocols such as RTMP (Real Time Messaging Protocol), HLS (Http Live Streaming), are required. In this paper, we analyzed vulnerability of paid live streaming services with the captured packets from the applications used by six major OTT (over-the-top) companies in Korea supporting live streaming services. We found that streaming channels were not encrypted and access control mechanisms were not properly used. Thus, guest users can freely use paid live streaming services.

Expansion of Human Tregs from Cryopreserved Umbilical Cord Blood for GMP-Compliant Autologous Adoptive Cell Transfer Therapy

Umbilical cord blood is a traditional and convenient source of cells for hematopoietic stem cell transplantation. Thymic regulatory T cells (Tregs) are also present in cord blood, and there is growing interest in the use of autologous Tregs to provide a low-risk, fully human leukocyte antigen (HLA)-matched cell product for treating autoimmune diseases, such as type 1 diabetes. Here, we describe a good manufacturing practice (GMP)-compatible Treg expansion protocol using fluorescence-activated cell sorting, resulting in a mean 2,092-fold expansion of Tregs over a 16-day culture for a median yield of 1.26 × 109 Tregs from single-donor cryopreserved units. The resulting Tregs passed prior clinical trial release criteria for Treg purity and sterility, including additional rigorous assessments of FOXP3 and Helios expression and epigenetic analysis of the FOXP3 Treg-specific demethylated region (TSDR). Compared with expanded adult peripheral blood Tregs, expanded cord blood Tregs remained more naive, as assessed by continued expression of CD45RA, produced reduced IFN-γ following activation, and effectively inhibited responder T cell proliferation. Immunosequencing of the T cell receptor revealed a remarkably diverse receptor repertoire within cord blood Tregs that was maintained following in vitro expansion. These data support the feasibility of generating GMP-compliant Tregs from cord blood for adoptive cell transfer therapies and highlight potential advantages in terms of safety, phenotypic stability, autoantigen specificity, and tissue distribution.

Microtubule density and landing rate as parameters to analyze tau protein in the MT-kinesin “gliding” assay

Microtubule-associated protein (MAP) tau is a well-established hallmark of a large group of age related neurodegenerative diseases collectively called tauopathies. Under pathological conditions the equilibrium of tau binding to the MTs is perturbed, either by misregulation in the expression levels of specific tau isoforms or by MAPT gene mutations. Preclinical detection of such misregulated tau proteins in cerebrospinal fluid (CSF) is desirable for differential diagnosis and effective prognosis of neurodegeneration. Conventional tau protein detection methods utilize tau isoform-specific antibodies. Such immuno-based protocols, including enzyme-linked immunosorbent assay (ELISA) and Western blots have appropriate sensitivity and specificity, but often show high variability and are time consuming. Here, we established a non-immuno tau protein detection method utilizing microtubule (MT)-kinesin “gliding”assay. All the six tau isoforms expressed in the human brain (0N3R, 1N3R, 2N3R, 0N4R, 1N4R and 2N4R) and five MAPT gene mutants (V248L, G272V, P301L, V337M and R406W) were studied. The landing rate, binding density and gliding velocity of MTs with respect to each tau type were determined and are proposed as tau detection parameters. The detection parameters depicted the type of tau bound to the MTs. Furthermore, MT landing rate and density were found to be superior to gliding velocity in differentiating tau isoforms and mutants. The 3R vs. 4R isoforms, their admixtures, wild vs. mutant 2N4R and specific mutants were differentiated. Our data show that MT-kinesin gliding assay provides a convenient, lab-on-a-chip (LOC) compatible and antibody-free protocol for tau protein analysis.

Abstracts from The 34(th) Annual National Neurotrauma Symposium June 26-29, 2016 Lexington, Kentucky.

Abstracts from The 34(th) Annual National Neurotrauma Symposium June 26-29, 2016 Lexington, Kentucky.

Research on Probabilistic Model based Services Composition Compatibility Focusing on Structural Behaviors Modeling

With the increasing of Web services on Internet, services composition requests considering not only the static information compatibility, such as interface grammar and semantic, but also the structural behavior compatibility, i.e., control flow and communication protocol. However, due to the uncertainty of Internet, application failures occurred in service-based software will cause kinds of economic losses. This stochastic phenomenon has been impacted on the structural behavior which makes services composition should take the probabilistic compatibility into account. The probabilistic compatibility needs to compute the degree of compatibility for the effective performance evaluation, which has been an important issue during services collaboration. In this paper, the probabilistic interface automaton is proposed to formalize service behaviors. Then, the synchronized product model is used to the qualitative checking for verifying whether they can interact with each other or not. Third, the probabilistic compatibilities of composite service and component service are discussed for the quantitative computing. Our method provides a reference to generate the correctness and reliable services composition.

What If? Re-imagined Scenarios and the Re-Virtualisation of History

What If? Re-imagined Scenarios and the Re-Virtualisation of History

Internet of Robotic Things: Concept, Technologies, and Challenges

Internet of Things allow massive number of uniquely addressable “things” to communicate with each other and transfer data over existing internet or compatible network protocols. This paper proposes a new concept which tackles the issues for supporting control and monitoring activities at deployment sites and industrial automations, where intelligent things can monitor peripheral events, induce sensor data acquired from a variety of sources, use ad hoc, local, and distributed “machine intelligence” to determine appropriate course of actions, and then act to control or disseminate static or dynamic position aware robotic things in the physical world through a seamless manner by providing a means for utilizing them as Internet of robotic things (IoRT). Although progressive advancements can be seen in multi-robotic systems, robots are constantly getting enriched by easier developmental functionalities, such vertical robotic service centric silos are not enough for continuously and seamlessly supporting for which they are meant. In this paper, a novel concept-IoRT is presented that highlights architectural principles, vital characteristics, as well as research challenges. The aim of this paper is to provide a better understanding of the architectural assimilation of IoRT and identify important research directions on this term.

In vitro co-culture model of medulloblastoma and human neural stem cells for drug delivery assessment

Physiologically relevant in vitro models can serve as biological analytical platforms for testing novel treatments and drug delivery systems. We describe the first steps in the development of a 3D human brain tumour co-culture model that includes the interplay between normal and tumour tissue along with nutrient gradients, cell-cell and cell-matrix interactions. The human medulloblastoma cell line UW228-3 and human foetal brain tissue were marked with two supravital fluorescent dyes (CDCFDASE, Celltrace Violet) and cultured together in ultra-low attachment 96-well plates to form reproducible single co-culture spheroids (d=600μm, CV%=10%). Spheroids were treated with model cytotoxic drug etoposide (0.3–100μM) and the viability of normal and tumour tissue quantified separately using flow cytometry and multiphoton microscopy. Etoposide levels of 10μM were found to maximise toxicity to tumours (6.5% viability) while stem cells maintained a surviving fraction of 40%. The flexible cell marking procedure and high-throughput compatible protocol make this platform highly transferable to other cell types, primary tissues and personalised screening programs. The model's key anticipated use is for screening and assessment of drug delivery strategies to target brain tumours, and is ready for further developments, e.g. differentiation of stem cells to a range of cell types and more extensive biological validation.

Research and Application of Facility Agriculture Monitoring and Early Warning Technology

Infrastructure changes in the environment have had a huge impact for the growth of crops. For real-time monitoring facilities environment, increase crop yield, the paper takes Jilin agricultural facilities, for example, introduced the greenhouses, sheds and other crops growing environmental monitoring network structures, technical methods of data acquisition and wireless transmission of real-time early warning information dissemination. This method realizes the different sensors compatible protocols and networking technology, the remote server through the unified management of data, enabling real-time alert when data anomalies. Application of the technology, which enables remote monitoring and early warning function effectively solve Jilin agricultural facilities centralized management, improve the level of facility agriculture park management and the ability to deal with abnormal.

PTU-122 A Gmp Treg Expansion Protocol Restores Treg Suppressor Function In End-stage Liver Disease; Implications For Adoptive Transfer Therapy

IntroductionLong-term survival in liver transplant recipients remains suboptimal because of the morbidity and mortality associated with the long-term use of immunosuppression (IS). However, IS weaning early post liver transplantation (LT)...

Development and validation of a fully GMP-compliant production process of autologous, tumor-lysate-pulsed dendritic cells

Background and aimsOne of the major challenges of dendritic cell (DC) vaccination is the establishment of harmonized DC production protocols. Here, we report the transfer and validation of a successfully used open DC manufacturing method into a closed system, good manufacturing practice (GMP)-compatible protocol. MethodsAll production steps (lysate generation, monocyte selection, DC culture and cryopreservation) were standardized and validated. ResultsTumor lysate was characterized by histology, mechanically homogenized and avitalized. This preparation yielded a median of 58 ± 21 μg protein per milligram of tumor tissue. Avitality was determined by trypan blue staining and confirmed in an adenosine triphosphate release assay. Patient monocytes were isolated by elutriation or CD14 selection, which yielded equivalent results. DCs were subsequently differentiated in Teflon bags for an optimum of 7 days in CellGro medium supplemented with interleukin (IL)-4 and granulocyte macrophage colony stimulating factor and then matured for 48 h in tumor necrosis factor-α and IL-1ß after pulsing with tumor lysate. This protocol resulted in robust and reproducible upregulation of DC maturation markers such as cluster of differentiation (CD)80, CD83, CD86, human leukocyte antigen-DR and DC-SIGN. Functionality of these DCs was shown by directed migration toward C-C motif chemokine ligand 19/21, positive T-cell stimulatory capacity and the ability to prime antigen-specific T cells from naive CD8+ T cells. Phenotype stability, vitality and functionality of DCs after cryopreservation, thawing and washing showed no significant loss of function. Comparison of clinical data from 146 patients having received vaccinations with plate-adherence versus GMP-grade DCs showed no inferiority of the latter. ConclusionsOur robust, validated and approved protocol for DC manufacturing forms the basis for a harmonized procedure to produce cancer vaccines, which paves the way for larger multi-center clinical trials.

Characterizing normal-use temperature conditions of pumped insulin.

In this study, the temperature profiles of insulin pump reservoirs during normal wear conditions across multiple seasons were characterized. Thermocouples secured in reservoirs filled with insulin diluent were loaded in infusion pumps worn by volunteers. Reservoir and ambient environmental temperature data and activity levels were logged during the course of normal daily activities in February (winter), April (spring), and August (summer). Each seasonal data set comprised 7 to 14 days of wear from 3 to 5 volunteers. Reservoir temperature profiles were generally higher than ambient temperatures, likely due to heat transfer from the wearer when the pump was placed close to the body. Temperature conditions inside pump reservoirs fluctuated between 25°C and 37°C regardless of seasonal variations. The average reservoir temperature remained close to 30°C across all seasons, notably lower than used in previously published compatibility and stability protocols (37°C). Results from this study could be utilized to develop more accurate stability and compatibility testing procedures for new insulin formulations and/or delivery devices.

Novel GMP-Compatible Protocol Employing an Allogeneic B Cell Bank for Clonal Expansion of Allospecific Natural Regulatory T Cells

The adoptive transfer of natural regulatory T cells (nTreg) is a new option to reshape undesired immune reactivity in autoimmunity and transplantation toward "tolerance." The first clinical trials using adoptive transfer of polyclonal nTreg demonstrated safety and hints of efficacy. However, the low frequencies of antigen-specific cells among the pool of polyclonal nTreg and their broad antigen nonspecific suppression are limitations of this approach regarding efficacy and safety. Recently, the isolation and expansion of (allo)antigen-specific nTreg have successfully been achieved by using Treg-specific activation markers but the yield is relatively low. Here, we describe a novel good manufacturing practice (GMP)-compatible expansion protocol of alloantigen-specific nTreg based on the stimulation of nTreg by allogeneic activated B cells. Their functionality and specificity are superior compared to polyclonal nTreg both in vitro and in vivo. Employing an allogeneic B cell bank, designed to cover the majority of HLA types, allows fast GMP-compliant manufacturing for donor-specific nTreg for clinical application in organ and stem cell transplantation. TCR repertoire analyses by next generation sequencing revealed impressive expansion by several log-steps of even very low-abundance alloantigen-specific nTreg clones. This novel method offers a simple approach for expanding antigen-specific nTreg and is characterized by high replicability and easy transferability to full GMP standards.

Two-hop incentive compatible routing protocol in disruption-tolerant networks

Two-hop incentive compatible routing protocol in disruption-tolerant networks

Rapid extraction and preservation of genomic DNA from human samples

Simple and rapid extraction of human genomic DNA remains a bottleneck for genome analysis and disease diagnosis. Current methods using microfilters require cumbersome, multiple handling steps in part because salt conditions must be controlled for attraction and elution of DNA in porous silica. We report a novel extraction method of human genomic DNA from buccal swab and saliva samples. DNA is attracted onto a gold-coated microchip by an electric field and capillary action while the captured DNA is eluted by thermal heating at 70 °C. A prototype device was designed to handle four microchips, and a compatible protocol was developed. The extracted DNA using microchips was characterized by qPCR for different sample volumes, using different lengths of PCR amplicon, and nuclear and mitochondrial genes. In comparison with a commercial kit, an equivalent yield of DNA extraction was achieved with fewer steps. Room-temperature preservation for 1 month was demonstrated for captured DNA, facilitating straightforward collection, delivery, and handling of genomic DNA in an environment-friendly protocol.

ATCA/AXIe compatible board for fast control and data acquisition in nuclear fusion experiments

An in-house development of an Advanced Telecommunications Computing Architecture (ATCA) board for fast control and data acquisition, with Input/Output (IO) processing capability, is presented. The architecture, compatible with the ATCA (PICMG 3.4) and ATCA eXtensions for Instrumentation (AXIe) specifications, comprises a passive Rear Transition Module (RTM) for IO connectivity to ease hot-swap maintenance and simultaneously to increase cabling life cycle.The board complies with ITER Fast Plant System Controller (FPSC) guidelines for rear IO connectivity and redundancy, in order to provide high levels of reliability and availability to the control and data acquisition systems of nuclear fusion devices with long duration plasma discharges.Simultaneously digitized data from all Analog to Digital Converters (ADC) of the board can be filtered/decimated in a Field Programmable Gate Array (FPGA), decreasing data throughput, increasing resolution, and sent through Peripheral Component Interconnect (PCI) Express to multi-core processors in the ATCA shelf hub slots. Concurrently the multi-core processors can update the board Digital to Analog Converters (DAC) in real-time. Full-duplex point-to-point communication links between all FPGAs, of peer boards inside the shelf, allow the implementation of distributed algorithms and Multi-Input Multi-Output (MIMO) systems. Support for several timing and synchronization solutions is also provided.Some key features are onboard ADC or DAC modules with galvanic isolation, Xilinx Virtex 6 FPGA, standard Dual Data Rate (DDR) 3 SODIMM memory, standard CompactFLASH memory card, Intelligent Platform Management Controller (IPMC), two PCI Express x4 (generation 2) ATCA Fabric channels (dual-star topology), eleven Xilinx Aurora x1 (or other ATCA compatible communications protocol) ATCA fabric channels (full-mesh topology) and two Fast Ethernet (Precision Time Protocol – PTP IEEE1588-V2 and Lan eXtensions for Instrumentation – LXI compatible) ATCA base channels (dual-star topology).

Simultaneous determination of vitamin B1 and B2 in complex cereal foods, by reverse phase isocratic HPLC-UV

The evaluation of nutritional or functional components in grain products is an important feature for the industry, especially when recent regulations require a correct nutrition labelling, valid during all the shelf life of the product. For that reason, industry usually makes many efforts to develop simple and reliable analytical methods that can be easily applied in any quality control laboratories for routine analysis. Spectrofluorimetric analysis of thiamine and riboflavin are sensitive, but need specific equipment. A few HPLC-UV methods have been described but they are less sensitive, and present difficulties due to interfering compounds, particularly in complex food matrixes, as grains and derivatives.A combination of extraction and separation systems, that allows enough sensitivity, precision and accuracy for the analysis of vitamin B1 and B2 in complex cereal food products, by isocratic UV-HPLC, in a single wavelength simultaneous separation is presented, with the advantage of using low-cost equipment requirements, simple sample pre-treatment and short time. The achievement of this goal has involved the optimization of compatible extraction and measurement protocols for cereal matrices, comparing seven different separation conditions and six extraction/clarification matrices analysis. The selected method was comparatively validated and compared to reference AOAC spectrofluorimetric methods, providing comparable linearity and accuracy, with better specificity and precision parameters, as well as practical applicability.