Community In Leachate Research Articles

Microbial community composition and metabolic functions in landfill leachate from different landfills of China

Landfill leachate usually harbors complex microbial communities responsible for the decomposition of municipal solid waste. However, the diversity and metabolic functions of the microbial communities in landfill leachate as well as the factors that influence them are still not well understood. In this study, Illumina MiSeq high-throughput sequencing was used to investigate the microbial community composition and metabolic functions in landfill leachate from 11 cities in China. The microbial diversity and structure of different leachate samples exhibited obvious differences. In general, Bacteroidetes, Firmicutes and Proteobacteria were the three dominant microbial communities among the 26 bacterial phyla identified in landfill leachate, regardless of the geographical locations. Diverse bacterial genera associated with various functions such as cellulolytic bacteria (e.g., Sphaerochaeta and Defluviitoga), acidifying bacteria (e.g., Prevotella and Trichococcus) and sulfate-reducing bacteria (e.g., Desulfuromonas and Desulfobacterium) were detected abundantly in the landfill leachate. Moreover, the archaeal community in all leachate samples was dominated by the orders Methanomicrobiales and Methanosarcinales belonging to the Euryarchaeota phylum. Notably, the archaea-specific primer pair covered more diverse archaeal communities than the universal bacteria-archaea primer pair. Seventeen archaeal genera belonging to acetoclastic, hydrogenotrophic, and methylotrophic methanogens were identified, and the composition of the dominant genera in these samples varied greatly. The canonical correlation analysis indicated that landfill age, electrical conductivity, ammonia nitrogen, and total nitrogen were significantly correlated with the microbial community structure. Based on PICRUSt2, a total of 41 metabolic pathways belonging to six metabolic pathway groups were predicted, and the KEGG pathway Metabolism was the most abundant group across all leachate samples. This study provides an important insight into the composition and functional characteristics of the microbial communities in landfill leachate.

Effect of cathode material and charge loading on the nitrification performance and bacterial community in leachate treating Electro-MBRs

Electro-MBR technology, which combines an electrocoagulation process inside the mixed liquor of a membrane bioreactor, was studied for the treatment of a high-strength ammonia leachate (124 ± 4 mg NH4-N L−1). A lab-scale aerobic Electro-MBR was operated with a solid retention time of 45 days, hydraulic retention times of 24h and 12h, and charge loading ranging from 100 to 400 mAh L−1. At 400 mAh L−1, with a combination of a Ti/Pt cathode and a sacrificial iron anode, removal percentages for ammonia nitrogen, total organic carbon, and total phosphorus were 99.8%, 38%, and 99.0%, respectively. At 400 mAh L−1, the estimated ferric ion dosage was 325 mg Fe3+ L−1. Experiments conducted with different cathode materials showed that previously reported inhibition phenomena may result from a cathodic nitrate reduction into ammonia nitrogen. Conventional cathode materials, such as graphite, have electrochemical nitrate reduction rates of −0.03 mg NO3-N mAh−1. By comparison, when using Ti/Pt, the rate was −0.0045 mg NO3-N mAh−1(85% lower than graphite due to its low hydrogen overpotential). Charge loading tested in this study had no significant impact on both nitrification performance and microbial population diversity. However, the relative abundance of the mixed liquor’s Nitrosomonas increased from 4.8% to 8.2% when the charge loading increased from 0 to 400 mAh L−1. Results from this study are promising for future applications of the Ti/Pt - Iron Electro-MBR in various high-strength ammonia wastewater treatment applications.

Comparison of bacterial communities in leachate from decomposing bovine carcasses.

ObjectiveBurial is associated with environmental effects such as the contamination of ground or surface water with biological materials generated during the decomposition process. Therefore, bacterial communities in leachates originating from the decomposing bovine carcasses were investigated.MethodsTo understand the process of bovine (Hanwoo) carcass decomposition, we simulated burial using a lab-scale reactor with a volume of 5.15 m3. Leachate samples from 3 carcasses were collected using a peristaltic pump once a month for a period of 5 months, and bacterial communities in samples were identified by pyrosequencing of the 16S rRNA gene.ResultsWe obtained a total of 110,442 reads from the triplicate samples of various sampling time points (total of 15 samples), and found that the phylum Firmicutes was dominant at most sampling times. Differences in the bacterial communities at the various time points were observed among the triplicate samples. The bacterial communities sampled at 4 months showed the most different compositions. The genera Pseudomonas and Psychrobacter in the phylum Proteobacteria were dominant in all of the samples obtained after 3 months. Bacillaceae, Clostridium, and Clostridiales were found to be predominant after 4 months in the leachate from one carcass, whereas Planococcaceae was found to be a dominant in samples obtained at the first and second months from the other two carcasses. The results showed that potentially pathogenic microbes such as Clostridium derived from bovine leachate could dominate the soil environment of a burial site.ConclusionOur results indicated that the composition of bacterial communities in leachates of a decomposing bovine shifted continuously during the experimental period, with significant changes detected after 4 months of burial.

Bacterial community composition and abundance in leachate of semi-aerobic and anaerobic landfills

The abundance and phylogenetic composition of bacterial community in leachate of semi-aerobic and anaerobic landfill were compared through real-time polymerase chain reaction and denaturing gradient gel electrophoresis. In semi-aerobic landfill scenario, the bacterial 16S rRNA copy numbers in leachate had no significant reduction from initial stage to stable period. In the scenario of anaerobic landfill, the largest bacterial 16S rRNA gene copy number was found in leachate at initial stage, but it reduced significantly at stable period. Moreover, methane-oxidizing bacteria population in stable period was lower than that in initial period in both two landfill processes. However, semi-aerobic landfill leachate had more methanotrophic bacteria populations than that in the anaerobic one. Furthermore, according to the sequences and phylogenetic analysis, obvious difference could be detected in bacterial community composition in different scenarios. Proteobacteria and bacteroidetes took up a dominantly higher proportion in semi-aerobic landfill leachate. To summarize up, different landfill methods and its landfill ages had crucial impacts on bacterial abundance and composition in leachate of semi-aerobic and anaerobic landfills.

Diversity and structure of the archaeal community in the leachate of a full-scale recirculating landfill as examined by direct 16S rRNA gene sequence retrieval.

The diversity and structure of the archaeal community in the effluent leachate from a full-scale recirculating landfill was characterized by direct 16S rRNA gene (16S rDNA) retrieval. Total-community DNA was extracted from the microbial assemblages in the landfill leachate, and archaeal 16S rDNAs were amplified with a universally conserved primer and an Archaea-specific primer. The amplification product was then used to construct a 16S rDNA clone library, and 70 randomly selected archaeal clones in the library were grouped by restriction fragment length polymorphism (RFLP) analysis. Sequencing and phylogenetic analysis of representatives from each unique RFLP type showed that the archaeal library was dominated by methanogen-like rDNAs. Represented in the kingdom of Euryarchaeota were phylotypes highly similar to the methanogenic genera Methanoculleus, Methanosarcina, Methanocorpusculum, Methanospirillum and Methanogenium, where the clone distribution was 48, 11, 3, 1 and 1, respectively. No sequences related to known Methanosaeta spp. were retrieved. Four rDNA clones were not affiliated with the known methanogenic Archaea, but instead, they were clustered with the uncultured archaeal sequences recently recovered from anaerobic habitats. Two chimeric sequences were identified among the clones analyzed.

Diversity and structure of the archaeal community in the leachate of a full-scale recirculating landfill as examined by direct 16S rRNA gene sequence retrieval

The diversity and structure of the archaeal community in the effluent leachate from a full-scale recirculating landfill was characterized by direct 16S rRNA gene (16S rDNA) retrieval. Total-community DNA was extracted from the microbial assemblages in the landfill leachate, and archaeal 16S rDNAs were amplified with a universally conserved primer and an Archaea-specific primer. The amplification product was then used to construct a 16S rDNA clone library, and 70 randomly selected archaeal clones in the library were grouped by restriction fragment length polymorphism (RFLP) analysis. Sequencing and phylogenetic analysis of representatives from each unique RFLP type showed that the archaeal library was dominated by methanogen-like rDNAs. Represented in the kingdom of Euryarchaeota were phylotypes highly similar to the methanogenic genera Methanoculleus, Methanosarcina, Methanocorpusculum, Methanospirillum and Methanogenium, where the clone distribution was 48, 11, 3, 1 and 1, respectively. No sequences related to known Methanosaeta spp. were retrieved. Four rDNA clones were not affiliated with the known methanogenic Archaea, but instead, they were clustered with the uncultured archaeal sequences recently recovered from anaerobic habitats. Two chimeric sequences were identified among the clones analyzed.