Communities In Water Column Research Articles

Vertical profiles of community and activity of methanotrophs in large lake and reservoir of Southwest China

Microbial methane oxidation plays a significant role in regulating methane emissions from lakes and reservoirs. However, the differences in methane oxidation activity and methanotrophic community between lakes and reservoirs remain inadequately characterized. In this study, sediment and water samples were collected from the large shallow lake (Dianchi) and deep reservoirs (Dongfeng and Hongjiadu) located in karst area, Southwest China. The results indicated that the rates of aerobic oxidation of methane (AeOM) in lake sediment ranged from 7.1 to 27.7 μg g−1 d−1, which was higher than that in reservoirs sediment (1.92 to 11.56 μg g−1 d−1). Similarly, the average AeOM in the water column of lake (104.7 μg L−1 d−1) was much higher than that of reservoirs (46 μg L−1 d−1). The content of sediment organic carbon and dissolved inorganic carbon were important factors that influenced the rates of AeOM in sediment and water column, respectively. 16S rRNA genes sequencing revealed a higher relative abundance of methanotrophs in lake sediments compared to reservoir sediments. The dominant methanotrophic taxa in lake was Methylococcaceae (type Ib), while Methylomonadaceae (type Ia) was predominant in reservoirs. Meanwhile, anaerobic methane-oxidizing microorganisms Candidatus Methylomirabilis and Candidatus Methanoperedens were also abundant in sediments of reservoirs. However, metatranscriptomic analysis revealed that the type I methanotrophs, especially Methylobacter, was most active in the sediment of both lake and reservoir. Water depth and conductivity could be the key controlling factors of the structures of methanotrophic communities in sediment and water column, respectively. Metagenome-assembled genomes suggested that type I methanotrophs exhibited greater motility, as evidenced by a higher number of flagellar assembly genes, while type II methanotrophs demonstrated advantages in metabolic processes such as carbon, phosphorus, and methane metabolism.

A bacteria-based index of biotic integrity assesses aquatic ecosystems effectively in rewetted long-term dry river channel after water replenishment.

Climate-induced droughts exert a significant influence on the connectivity of river systems. It is estimated that about 25% of the world's rivers ran dry before reaching the ocean due to climate change and human activities. Ecological water replenishment is an effective measure for restoring aquatic ecosystems damaged by drought. It is urgently needed to quantitatively assess the aquatic ecosystems in rewetted dry river channels after water replenishment. This study investigated the variations in phytoplankton, zooplankton, benthic macroinvertebrates, and benthic bacterial communities in the rewetted dry river channel of Yongding River after water replenishment. In comparison with the water column communities, the benthic macroinvertebrates were identified as limiting factors for ecological restoration in rewetted dry river channels. In the absence of a certain recovery time for benthic macroinvertebrates, the benthic bacterial-based index of biological integrity, especially calculated based on their intrinsic properties, can properly assess aquatic ecosystems in rewetted dry river channels.

Microeukaryotic Communities of the Long-Term Ice-Covered Freshwater Lakes in the Subarctic Region of Yakutia, Russia

Currently, microeukaryotic communities of the freshwater arctic and subarctic ecosystems are poorly studied. Still, these are of considerable interest due to the species biogeography and autecology as well as global climate change. Here, we used high-throughput 18S rRNA amplicon sequencing to study the microeukaryotic communities of the large subarctic freshwater lakes Labynkyr and Vorota in Yakutia, Russia, during the end of the ice cover period, from April to June. By applying the statistical methods, we coupled the microeukaryotic community structure profiles with available discrete factor variables and hydrophysical, hydrochemical, and environmental parameters. The sub-ice layer and the water column communities were differentiated due to the temporal change in environmental conditions, particularly temperature regime and electric conductivity. Additionally, the community composition of unicellular eukaryotes in lakes Labynkyr and Vorota was changing due to seasonal environmental factors, with these alterations having similar patterns in both sites. We suggest the community developed in the sub-ice layer in April serves as a primer for summer freshwater microeukaryotes. Our results extend the current knowledge on the community composition and seasonal succession of unicellular eukaryotes within subarctic freshwater ecosystems.

Comparing diversity patterns and processes of microbial community assembly in water column and sediment in Lake Wuchang, China.

The study compare the diversity patterns and processes of microbial community assembly in the water and sediment of Lake Wuchang (China) using high-throughput sequencing of 16S rRNA gene amplicons. A higher microbial α-diversity in the sediment was revealed (P < 0.01), and the most common bacterial phyla in water column were Proteobacteria, Cyanobacteria and Actinobacteria, while Proteobacteria, Acidobacteria, Chloroflexi and Nitrospirae were dominant in sediment. Functions related to phototrophy and nitrogen metabolism primarily occurred in the water column and sediment, respectively. The microbial communities in water column from different seasons were divided into three groups, while no such dispersion in sediment based on PCoA and ANOSIM. According to Pearson correlation analysis, water temperature, dissolved oxygen, water depth, total nitrogen, ammonium, and nitrite were key factors in determining microbial community structure in water column, while TN in sediment, conductivity, and organic matter were key factors in sediment. However, the stochastic processes (|βNTI| < 2) dominated community assembly in both the water column and sediment of Lake Wuchang. These data will provide a foundation for microbial development and utilization in lake water column and sediment under the circumstances of increasing tendency of lake ecological fishery in China.

Bacterial communities in a subarctic stream network: Spatial and seasonal patterns of benthic biofilm and bacterioplankton.

Water-column bacterial communities are assembled by different mechanisms at different stream network positions, with headwater communities being controlled by mass effects (advection of bacteria from terrestrial soils) while downstream communities are mainly driven by environmental sorting. Conversely, benthic biofilms are colonized largely by the same set of taxa across the entire network. However, direct comparisons of biofilm and bacterioplankton communities along whole stream networks are rare. We used 16S rRNA gene amplicon sequencing to explore the spatiotemporal variability of benthic biofilm (2 weeks old vs. mature biofilm) and water-column communities at different network positions of a subarctic stream from early summer to late autumn. Amplicon sequence variant (ASV) richness of mature biofilm was about 2.5 times higher than that of early biofilm, yet the pattern of seasonality was the same, with the highest richness in midsummer. Biofilm bacterial richness was unrelated to network position whereas bacterioplankton diversity was negatively related to water residence time and distance from the source. This pattern of decreasing diversity along the network was strongest around midsummer and diminished greatly as water level increased towards autumn. Biofilm communities were phylogenetically clustered at all network positions while bacterioplankton assemblages were phylogenetically clustered only at the most downstream site. Both early and mature biofilm communities already differed significantly between upstream (1st order) and midstream (2nd order) sections. Network position was also related to variation in bacterioplankton communities, with upstream sites harbouring substantially more unique taxa (44% of all upstream taxa) than midstream (20%) or downstream (8%) sites. Some of the taxa that were dominant in downstream sections were already present in the upmost headwaters, and even in riparian soils, where they were very rare (relative abundance <0.01%). These patterns in species diversity and taxonomic and phylogenetic community composition of the riverine bacterial metacommunity were particularly strong for water-column communities, whereas both early and mature biofilm exhibited weaker spatial patterns. Our study demonstrated the benefits of studying bacterioplankton and biofilm communities simultaneously to allow testing of ecological hypotheses about biodiversity patterns in freshwater bacteria.

Exaggerated trans-membrane charge of ammonium transporters in nutrient-poor marine environments.

Transporter proteins are a vital interface between cells and their environment. In nutrient-limited environments, microbes with transporters that are effective at bringing substrates into their cells will gain a competitive advantage over variants with reduced transport function. Microbial ammonium transporters (Amt) bring ammonium into the cytoplasm from the surrounding periplasm space, but diagnosing Amt adaptations to low nutrient environments solely from sequence data has been elusive. Here, we report altered Amt sequence amino acid distribution from deep marine samples compared to variants sampled from shallow water in two important microbial lineages of the marine water column community-Marine Group I Archaea (Thermoproteota) and the uncultivated gammaproteobacterial lineage SAR86. This pattern indicates an evolutionary pressure towards an increasing dipole in Amt for these clades in deep ocean environments and is predicted to generate stronger electric fields facilitating ammonium acquisition. This pattern of increasing dipole charge with depth was not observed in lineages capable of accessing alternative nitrogen sources, including the abundant alphaproteobacterial clade SAR11. We speculate that competition for ammonium in the deep ocean drives transporter sequence evolution. The low concentration of ammonium in the deep ocean is therefore likely due to rapid uptake by Amts concurrent with decreasing nutrient flux.

Ecological Impacts of Aged Freshwater Biofilms on Estuarine Microbial Communities Elucidated Through Microcosm Experiments: A Microbial Invasion Perspective.

Inadvertent introductions of alien species via biofilms as a vector released through ballast water are of environmental importance, yet their consequences are not much known. In the present study, biofilm communities developed in an inland freshwater port under in situ and dark conditions were subjected to long-term dark incubations. Subsequently, the impact of these aged biofilms as vectors on estuarine water column communities were evaluated using microcosm experiments in the laboratory. Variations in biofilm and planktonic microbial communities were quantified using quantitative PCR.Upon prolonged dark incubation, a shift in bacterial diversity with an increase in tolerant bacterial communities better adapted to stress was observed. Actinobacteria were the dominant taxa in both aged biofilms upon dark incubations. The laboratory studies indicated that on exposure of these biofilms to estuarine water, resuscitation of Vibrio alginolyticus, V. parahaemolyticus, and V. cholerae from a dormant state existing in these biofilms to culturable form was observed. Moreover, the results revealed that both the biofilm types can pose a threat to the environment, but the degree of risk can be attributed to the imbalance caused by significant changes in the surrounding estuarine microbial communities. Consequently, this may result in either proliferation or decline of some genera with different metabolic potential and resuscitation of pathogenic forms not present earlier, thereby influencing the ecology of the environment. Quantifying these effects in the field using biofilm metagenomes with an emphasis on virulent species and understanding traits that enable them to adapt to changing environments is a way forward.

Microbial communities associated with sinking particles across an environmental gradient from coastal upwelling to the oligotrophic ocean

Complex interactions between microbial communities in the epipelagic and mesopelagic ocean drive variability in sinking carbon flux as part of the biological carbon pump. To investigate the relative contribution of dominant prokaryotic, protistan, and phytoplankton taxa to sinking particles, we used 16S/18S rRNA gene sequencing of particles collected in sediment traps and water column communities. Comparisons were done across a coastal upwelling-to-oligotrophic environmental gradient in the southern California Current Ecosystem to assess effects of natural environmental variability on taxon-specific contributions to sinking particle export. Particle-associated microbial assemblages differ from ambient mixed-layer communities. Gammaproteobacteria, dinoflagellates, and rhizarians were dominant microbes associated with sinking particles at all sampling locations, with diatoms increasing significantly at the inshore mesotrophic site. Parasitic groups, syndiniales and apicomplexans, were also major particle-associated taxa. Relative sequence abundance on exported particles from the mesotrophic inshore site more closely resembled the water-column dominants (especially diatoms) found above, while oligotrophic communities exhibited greater dissimilarity between the microbial communities on sinking particles and in the mixed layer. Protists generally showed greater similarity between mixed-layer and sinking particle communities than prokaryotes. Synechococcus was over-represented on sinking particles (relative to eukaryotic phytoplankton and other cyanobacteria) only in oligotrophic areas. Diatoms were the only phytoplankton group consistently over-represented on sinking particles across the region. Our results highlight important variability in microbial contributions to export that seem to align with differences in dominant grazing pathways. At the upwelling influenced site where export was primarily due to the fecal pellets of large copepods, diatoms were dominant contributors to export and protistan communities on sinking particles resembled those in the water column above. When doliolids were abundant, dinoflagellates contributed substantially to export, while the dominant picoeukaryote (Ostreococcus) was only a minor contributor. In oligotrophic areas, where grazing pathways were dominated by protists and sinking particles were primarily amorphous aged aggregates, there was little similarity between mixed layer and sinking particle communities.

Changes in microbial community phylogeny and metabolic activity along the water column uncouple at near sediment aphotic layers in fjords

Fjords are semi-enclosed marine systems with unique physical conditions that influence microbial community composition and structure. Pronounced organic matter and physical condition gradients within fjords provide a natural laboratory for the study of changes in microbial community structure and metabolic potential in response to environmental conditions. Photosynthetic production in euphotic zones sustains deeper aphotic microbial activity via organic matter sinking, augmented by large terrestrial inputs. Previous studies do not consider both prokaryotic and eukaryotic communities when linking metabolic potential and activity, community composition, and environmental gradients. To address this gap we profiled microbial functional potential (Biolog Ecoplates), bacterial abundance, heterotrophic production (3H-Leucine incorporation), and prokaryotic/eukaryotic community composition (16S and 18S rRNA amplicon gene sequencing). Similar factors shaped metabolic potential, activity and community (prokaryotic and eukaryotic) composition across surface/near surface sites. However, increased metabolic diversity at near bottom (aphotic) sites reflected an organic matter influence from sediments. Photosynthetically produced particulate organic matter shaped the upper water column community composition and metabolic potential. In contrast, microbial activity at deeper aphotic waters were strongly influenced by other organic matter input than sinking marine snow (e.g. sediment resuspension of benthic organic matter, remineralisation of terrestrially derived organic matter, etc.), severing the link between community structure and metabolic potential. Taken together, different organic matter sources shape microbial activity, but not community composition, in New Zealand fjords.

Effects of Concentrated Freshwater Discharge on Hydrography and Mesozooplankton Community in a Dammed Estuary

This study examines the different effects of high and low freshwater discharge on the water physical structure of the water column and plankton community in a tide-dominated eutrophic estuary, the upper reach of which has been dammed. Water with a low amount of dissolved oxygen (DO) rose to the surface in 2017, which was most likely due to tidal reflection. The surface DO level is also highly affected by the concentration of chlorophyll-a (Chl-a), which may increase the DO through photosynthesis. However, this effect seems to be minimal given the degree of stratification, and the freshwater appears to remain in the bay for less than 10 days according the progression in this macrotidal region. Following a freshwater discharge in 2012, there was an influx of ammonium into the bay, followed by an increase in Chl-a and mesozooplankton biomass. When the flow was lower in 2017, there were no cladocerans. Instead, a small copepod (Oithona similis) increased exponentially toward late August, potentially taking advantage of the empty niche and rapidly multiplied during the relatively low freshwater discharge. The low oxygen did not affect the mesozooplankton, which may have been due to the relatively high DO level in the bottom waters in both years.

Role of emergent and submerged vegetation and algal communities on nutrient retention and management in a subtropical urban stormwater treatment wetland

A 4.6-ha urban stormwater treatment wetland complex in southwest Florida has been investigated for several years to understand its nutrient retention dynamics. This study investigates the role of aquatic vegetation, both submerged vegetation (such as benthic macrophytic and algal communities) and emergent plant communities, on changes in nutrient fluxes through the wetlands. Gross and net primary productivity of water column communities and net primary productivity of emergent macrophytes were used to estimate nutrient fluxes through vegetation in these wetlands using biannual biomass, nutrient concentrations of plant material, and areal coverage data. Emergent macrophyte net primary productivity was estimated as the difference between the increase of productivity during the wet season and the loss during the dry season which, in turn, suggested approximately 0.11g-N m− 2 y− 1 and 0.09g-P m− 2 yr− 2 being removed, primarily from the soil, by emergent vegetation. Water column primary productivity accounted for a much larger flux of nutrients with approximately 39.6g-N m− 2 yr− 1 and 2.4g-P m− 2 yr− 1 retained in algal communities. These fluxes, combined with measurements in parallel studies, allowed us to develop preliminary nutrient budgets for these wetlands and identify gaps, or missing fluxes, in our models for these wetlands. The results further validated previous findings that suggested that there are large inputs of nitrogen (up to 62.3g-N m− 2 yr− 1) that are not accounted for by the pumped inflow. Additionally, management suggestions are provided to improve water quality by identifying vegetative species that are most effective at retaining nutrients.

Dark Ammonium Transformations in the Pearl River Estuary During Summer

AbstractGrowing human activities in recent decades have collectively resulted in large amounts of nutrients export into coastal oceans. As the most reactive nitrogen species, ammonium ( ) plays the critical role in biogeochemical cycles in estuaries and the coastal ocean. In the highly polluted Pearl River Estuary (PRE), predominates to be the energy source for nitrification and to be the material source for bacteria and phytoplankton to grow. Both above processes are affected by light, yet in opposite ways. Nevertheless, rare studies paid attention to dual transformation processes specifically during dark conditions. By using nitrogen isotope tracer technique, we quantitatively and simultaneously differentiated two distinctive consumption pathways, that is, oxidation (AOD) and assimilation (AAD) rates, especially under dark conditions along the PRE during the 2015 and 2017 summer cruises when biological activities were the highest. We found the transformations display a bilayer structure with AAD &gt; AOD in almost all the surface waters and vice versa in all bottom waters, suggesting bacteria and phytoplankton (mainly bacteria) control consumption in surface during the night while nitrifiers are the major consumer in the bottom waters. Through redundancy analysis, we found that both processes are mainly driven by in the PRE during summer. In addition, in the downstream PRE during two cruises, AOD at most contributed 27% and 8% of the water column community dissolved oxygen (DO) consumption assuming the Redfield stoichiometry has no direct effect on hypoxia formation in the PRE during summer.

Characterisation of constructed ponds and factors influencing their macroinvertebrate communities on the lower Waikato River floodplain, New Zealand

ABSTRACT Ponds are increasingly being constructed to enhance amenity values in human-dominated lowland landscapes, but little is known of design features that influence macroinvertebrates which can provide important food resources for fish and waterbirds. We quantified pond characteristics, sampled benthic and water-column macroinvertebrates in winter, and related abundance, biomass and community composition to characteristics of 34 ponds on the lower Waikato River floodplain, northern New Zealand. Compositionally different macroinvertebrate communities occupied water-column and benthic habitats. Landscape setting had a significant effect on benthic composition in terms of biomass, while the independent effects of frequency of pond drying or flooding, respectively, influenced benthic (abundance, biomass) or water-column (abundance) communities. Distance-based linear models highlighted physicochemical conditions, water depth regimes and riparian vegetation composition as key variables accounting for dissimilarity in total macroinvertebrate biomass between ponds. Our results indicate that managing hydrological stability of ponds in terms of permanence and flooding influences the composition and biomass of invertebrate communities. Within this hydrological template, design of appropriate depth regimes and riparian conditions can enhance accrual of macroinvertebrate biomass, thereby increasing food resources for fish and waterbirds.

Occurrence and distribution of microplastics in commercial fishes from estuarine areas of Guangdong, South China

The widespread presence of microplastics in global aquatic ecosystems has aroused growing concerns regarding their potential impact on aquatic biota. Data show an extensive plastic pollution in coastal areas of Guangdong, China, however, to date there has been no quantitative study on the ingestion of microplastics by commercial fishes from the estuarine areas. In this study, we analyzed the abundance, characteristics, species-specific and spatial distribution of microplastics in six species of commercial fish from estuarine areas of Guangdong. Sixty-four fish samples from nineteen sampling sites were investigated. The average abundance of microplastics in the gastrointestinal tracts of the commercial fishes was 5.4 items/individual, indicating potential food safety. The majority of microplastics were fibers, white in color and less than 1 mm in size. Species-specific and spatial distribution of the microplastics were observed in terms of abundance, shape, color and size. Sillago japonica had the highest (6.9 items/individual), while Alepes djedaba had the lowest (3 items/individual) abundance of microplastics in the gastrointestinal tract. We recommend demersal Mugil cephalus and pelagic Konosirus punctatus to be considered as two species suitable for monitoring microplastics ingestion in the seafloor and water column communities.

Bacterial and archaeal communities in deep sea waters near the Ninetyeast Ridge in Indian Ocean

Depth-dependent distribution patterns of bacterial and archaeal communities in deep sea water column around the Ninetyeast Ridge in the Indian Ocean were investigated using 16S rRNA gene profiling. Sampling was conducted at the northern Ninetyeast Ridge (1°59.89′N–9°59.70′S, 87°58.90′E–88°00.03′E) from September to November 2016 where samples were collected from the bathyal (1 000 m) to bathypelagic depths (>4 000 m) in four different stations. A total of 1 565 405 clean data falling into 6 712 bacterial OTUs and 1 452 727 clean data falling into 806 archaeal OTUs based on 97% similarity level were analyzed. Most of the bacterial 16S rRNA gene sequences were affiliated with Gammaproteobacteria, followed by Alphaproteobacteria and Bacteroidia. The archaeal 16S rRNA gene sequences mostly affiliated to Nitrososphaeria (Thaumarchaeota) dominated with relative abundances ranging from 52.68% to 97.2%, followed by Thermoplasmata (Euryarchaeota). Vertical partitioning of bacterial and archaeal communities among different water layers was observed. Canonical correspondence analysis (CCA) and Spearman’s correlations revealed that depth (P=0.003), dissolved oxygen (P=0.019), and nitrite (P=0.033) were the main environmental factors affecting bacterial community structure at genus level in the Ninetyeast Ridge. On the other hand, the first two CCA axes accounted for 74.4% of the explained total variance, it seems that the archaeal communities at genus level were heavily influenced by the environmental variables including depth, dissolved oxygen (DO), nitrite, salinity, phosphate, ammonia, nitrate, and silicate, but none of them exhibited any significant correlation on the structuring (P>0.1).

A managed realignment in the upper Bay of Fundy: Community dynamics during salt marsh restoration over 8 years in a megatidal, ice-influenced environment

Salt marshes are ecologically and globally vital ecosystems. Unfortunately, world-wide salt marsh loss has been extensive, and until recently there has been little effort to undo the loss of ~30,500 ha of salt marshes in Bay of Fundy, Canada, since European colonization. To better understand salt marsh restoration in the upper Bay of Fundy, we monitored sediment deposition and community dynamics in 2 managed realignment salt marsh restoration sites and 2 reference sites from 1 y pre-breach to 8 y post-breach in Aulac, New Brunswick. Because of the initial elevational disparity (~2 m) between site types, substantial amounts of sediment were deposited immediately after breaching the old dike (>50 cm in some locations). After 7–8 y, mean sediment deposition was 34–67 cm in the restoration sites, and 6 cm in a reference site. To date, we identified three stages of vegetative community succession: (i) rapid deposition of unconsolidated sediment and loss of terrestrial vegetation, but Spartina pectinata remained (1 y post-breach), (ii) colonization and spread of S. alterniflora and loss of S. pectinata (2–5 y post), and (iii) high percent cover and decreased spatial variability of S. alterniflora (mean stem density: 345 stems m−2, 6–8+ y post). We expect the fourth stage of vegetative community succession will be defined by spread of S. patens throughout restoration sites. Invertebrate community on emergent marsh and water column community in salt pools were variable and lagged behind vegetative community. Our study reported the first managed realignment in Maritime Canada, and the first such realignment in an ice-influenced and megatidal (~14 m tidal amplitude) region.

Functional properties of bacterial communities in water and sediment of the eutrophic river-lake system of Poyang Lake, China.

In river-lake systems, sediment and water column are two distinct habitats harboring different bacterial communities which play a crucial role in biogeochemical processes. In this study, we employed Phylogenetic Investigation of Communities by Reconstruction of Unobserved States to assess the potential functions and functional redundancy of the bacterial communities in sediment and water in a eutrophic river-lake ecosystem, Poyang Lake in China. Bacterial communities in sediment and water had distinct potential functions of carbon, nitrogen, and sulfur metabolisms as well as phosphorus cycle, while the differences between rivers and the lake were inconspicuous. Bacterial communities in sediment had a higher relative abundance of genes associated with carbohydrate metabolism, carbon fixation pathways in prokaryotes, methane metabolism, anammox, nitrogen fixation, and dissimilatory sulfate reduction than that of water column. Bacterial communities in water column were higher in lipid metabolism, assimilatory nitrate reduction, dissimilatory nitrate reduction, phosphonate degradation, and assimilatory sulfate reduction than that of sediment bacterial communities. Furthermore, the variations in functional composition were closely associated to the variations in taxonomic composition in both habitats. In general, the bacterial communities in water column had a lower functional redundancy than in sediment. Moreover, comparing to the overall functions, bacterial communities had a lower functional redundancy of nitrogen metabolism and phosphorus cycle in water column and lower functional redundancy of nitrogen metabolism in sediment. Distance-based redundancy analysis and mantel test revealed close correlations between nutrient factors and functional compositions. The results suggested that bacterial communities in this eutrophic river-lake system of Poyang Lake were vulnerable to nutrient perturbations, especially the bacterial communities in water column. The results enriched our understanding of the bacterial communities and major biogeochemical processes in the eutrophic river-lake ecosystems.

Cyanobacterial bloom mitigation by sanguinarine and its effects on aquatic microbial community structure

Sanguinarine has strong inhibitory effects against the cyanobacterium Microcystis aeruginosa. However, previous studies were mainly limited to laboratory tests. The efficacy of sanguinarine for mitigation of cyanobacterial blooms under field conditions, and its effects on aquatic microbial community structure remain unknown. To elucidate these issues, we carried out in situ cyanobacterial bloom mitigation tests. Our results showed that sanguinarine decreased population densities of the harmful cyanobacteria Microcystis and Anabaena. The inhibitory effects of sanguinarine on these cyanobacteria lasted 17 days, after which the harmful cyanobacteria recovered and again became the dominant species. Concentrations of microcystins in the sanguinarine treatments were lower than those of the untreated control except during the early stage of the field test. The results of community DNA pyrosequencing showed that sanguinarine decreased the relative abundance of the prokaryotic microorganisms Cyanobacteria, Actinobacteria, Planctomycetes and eukaryotic microorganisms of Cryptophyta, but increased the abundance of the prokaryotic phylum Proteobacteria and eukaryotic microorganisms within Ciliophora and Choanozoa. The shifting of prokaryotic microbial community in water column was directly related to the toxicity of sanguinarine, whereas eukaryotic microbial community structure was influenced by factors other than direct toxicity. Harmful cyanobacteria mitigation efficacy and microbial ecological effects of sanguinarine presented in this study will inform the broad application of sanguinarine in cyanobacteria mitigation.

Depth-Resolved Distribution of Particle-Attached and Free-Living Bacterial Communities in the Water Column of the New Britain Trench.

Particle-attached (PA) and free-living (FL) microorganisms play significant but different roles in mineralization of organic matter (OM) in the ocean. Currently, little is known about PA and FL microbial communities in bathyal and abyssal pelagic waters, and understanding of their diversity and distribution in the water column and their interactions with environmental factors in the trench area is limited. We investigated for the first time the variations of abundance and diversities of the PA and FL bacterial communities in the epi-, bathy-, and abyssopelagic zones of the New Britain Trench (NBT). The PA communities showed decreasing species richness but increasing relative abundance with depth, suggesting the increasing ecological significance of the PA bacteria in the deep ocean. The abundance and diversity of PA and FL bacterial communities in the NBT water column appeared to be shaped by different sets of environment factors, which might be related to different micro-niches of the two communities. Analysis on species distribution suggested that the differences between PA and FL bacteria communities mainly resulted from the different relative abundance of the “shared taxa” in the two types of communities. These findings provide valuable information for understanding the relative ecological roles of the PA and FL bacterial communities and their interactions with environmental factors in different pelagic zones along the vertical profile of the NBT water column.

Epiphytic Bacterial Communities in Seagrass Meadows of Oligotrophic Waters of Andaman Sea

The epiphytic microbial communities of seagrass (Cymodocea rotundata and Thalassia testudinum) along with sediments and seawater of the seagrass ecosystem of Andaman Sea were assessed for heterotrophic bacterial communities. Young leaves of C. nodosa and T. testudinum sediments and water samples were collected from two different locations at Burmanala and Havelock island. Young leaves were swabbed and transferred into Alkaline peptone water along with 1 gm of sediments and 1 ml of seawater for initial microbial enrichments, followed by culture of microbes in Salt Yeast Extract Agar. Thiosulphate Citrate Bile Salt agar was used for seagrass leaves, sediments and water for culturing of Vibrio like microbes. The observed colonies were morphologically (colour) identified first and then further identification was done by Gram staining and various biochemical tests. Sediment bacterial count was 10-fold higher than seagrass and water column communities. Morphologically 29% of total bacterial colonies identified were of grey and white colour. T. testudinum bacterial epiphytes were 2-fold higher than C. rotundata communities. Gram negative bacterial isolates of Pseudomonas, Vibrios and Aeromonas dominated the bacterial colonies than gram positive bacteria of Bacillus and Micrococcus. Vibrios were only observed on seagrass leaves. Seagrass harboured more gram positive bacterial colonies than gram negative, which were dominated in the sediments of seagrass ecosystem. The bacterial communities of landward site of Burmanala and Havelock were similar whereas 2-fold higher communities were observed at the seaward site of Havelock site on T. testudinum. Seagrass ecosystem bacterial epiphytic communities are diverse in oligotrophic waters.