Phase 2 of the Stimulating Peripheral Activity to Relieve Conditions (SPARC) program ( https://commonfund.nih.gov/sparc ) aims to build a comprehensive map of the anatomy and functional connectivity of the human vagus nerve, allowing comparisons of inter-subject variability, and helping guide experiments on vagus nerve stimulation and the development of neuromodulation devices. Following approaches used in Phase 1 of the SPARC program, segmented nerve and fascicle data (using various imaging modalities) of vagus nerves from a large number of human subjects will be registered into an Anatomical Scaffold. This is a geometric model of the vagus nerve providing a common coordinate system across subjects. Registration is achieved by fitting a subject-specific vagus scaffold to digitized subject data, using standardized annotations for trunks, branches, fixed landmarks and orientation marks in the data and for the corresponding features of the Scaffold. We report here on progress and issues in the early stages of the study. A particular complication of the vagus is inter-subject variability. While the left and right vagus trunk are quite consistent across subjects, the number and locations of branches innervating a particular organ or target is quite different from one subject to another. This dictates generating a scaffold for each subject with its own unique branching structure. Once data is available for a reasonable number of subjects these will be remapped to a common or representative vagus scaffold. At the conclusion of this study all vagus imaging, plus derived data including the scaffold and embedded fascicles, their groupings and distributions will be freely available on the SPARC Portal ( https://sparc.science/ ). Mapping to a common scaffold allows these data to be compared and visualized at equivalent locations across all subjects in the study. This work is supported by the NIH SPARC program under award number OT3OD025347. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.