Common Carp Eggs Research Articles

Vertical Transmission of Cyprinid Herpesvirus 3 (CyHV-3) in Common Carp Cyprinus carpio: Experimental Insights and Implications

Currently, little is known about vertical transmission of cyprinid herpesvirus 3 (CyHV-3). Here, we assessed potentials for (1) vertical transmission of CyHV-3 and (2) viral infection to fertilized eggs via viral-contaminated environmental water. After CyHV-3 exposure, eggs were incubated, and hatchlings were raised to juvenile stage. To assess occurrence of KHVD during the experiment, samplings were performed at various stages, such as fertilized egg, eyed egg, larval, and juvenile stages. The viral DNA was detected only from eggs at 3 h post fertilization, but not from fish at later stages. No mortality associated with CyHV-3 infection was observed in all the groups during the experimental period, or fish became 91 days post hatch. Additionally, those juveniles were re-exposed to CyHV-3 to investigate whether CyHV-3 infection was established at the egg stage. As a result, statistically significant difference was not observed in the cumulative mortality between the larval fish with or without CyHV-3 infection at the egg stage, suggesting that fish exposed to CyHV-3 at the egg stage did not acquire immunity against the viral infection. All our results suggest that it is improbable that KHVD can be transmitted vertically through eggs of common carp that have survived CyHV-3 infection.

Egg Hatching and Larval Survival of Common Carp (Cyprinus carpio Linnaeus, 1758) Againts Exposure to Carbamate Insecticides with Different Concentrations

The purpose of this study was to determine the value of Hours from the use of carbamate insecticides on hatchability of Common carp (Cyprinus carpio) eggs, to compare hatchability of carp (Cyprinus carpio) eggs exposed to carbamate insecticides at different concentration levels, to compare survival rates. goldfish exposed to carbamate insecticides at different concentration levels. This study used a completely randomized design (RAL) where there were 3 treatments and 1 control. In each treatment there were 3 replications. Data analysis used ANOVA and DNMRT tests. The results showed that the average percentage of goldfish egg hatchability was at a concentration of 0.4 ppm with 58% hatchability. While the percentage of survival is at a concentration of 0.4 ppm with hatchability of eggs is 56%. The results of acute toxicity test Hours of carp eggs are at a concentration of 0.8 ppm. The conclusion obtained is that the acute concentration of hours of carbamate pesticides with the active ingredient karbosulfan in Common carp eggs (Cyprinus carpio) is a concentration of 0.8 ppm. There are differences in egg hatchability and survival rate of carbamate (Cyprinus carpio) larvae on exposure to carbamate insecticides with different concentrations, and there is a tendency that the higher the concentration of carbamate insecticides used, the lower the hatchability of eggs and carbamate (Cyprinus carpio) larvae survival.

Sperm and egg in vitro storage effects on artificial fertilization and hatching in common carp (Cyprinus carpio L.)

This is the first report showing fertilization of short-term stored eggs (19 °C) with short-term stored diluted sperm (0–2 °C) in common carp (Cyprinus carpio). From 5 males, fresh (control) and 7-day stored sperm were diluted with common carp extender. After collection of eggs from nine females, the eggs were immediately pooled into three groups from females 1–3, 4–6 and 7–9, according to the eggs' quality based on visual criteria. They were placed in beakers, covered and incubated at 19 °C (for each pool, 10 g of eggs from each female were used). The 7-day short-term storage sperm was incubated at 20 °C for 20 min and pooled before being used for fertilization. The eggs were fertilized with fresh and short-term storage sperm after 1, 3 and 6 h post storage of eggs and activated with hatchery water and Perchec solution. Sperm motility and velocity (50–54% and 80–115 µm/s) of 7-day short-term storage sperm at 0–2 °C were ensured by additional incubation of sperm at 20 °C for 20 min before being activated. Principal component analysis showed that the sorting of eggs of different females into three groups according to sperm quality, fertilization ability and production of malformed larvae was successful. It is recommended that overripe common carp eggs are stored for a maximum of 1 h. A period of 3 h can be used for better quality eggs and exceptionally, up to 6 h for very high-quality eggs. Higher larvae malformation was found in the 6 h aged eggs together with fertilized short-term storage and fresh sperm. In all quality groups of eggs, the effect of different sperm, activation medium and egg storage time on the level of malformations was observed. The activation solution for carp did not show any positive fertilization and hatching effects.

Comparative ultrastructure and proteomics of two economic species (common carp and grass carp) egg envelope

Progressive morphological and cytochemical processes occur in the egg after fertilization in which the egg envelope changes dramatically. However, the biochemical and structural alterations of egg envelope between common carp adhesive eggs and grass carp semi-buoyant eggs remain poorly understand. Here, this study found that compared with grass carp semi-buoyant eggs, common carp adhesive eggs possess a smaller egg diameter and a thicker egg envelope, and the difference was most significant at 1 h postfertilization. After fertilization, an adhesive layer was found and gradually thickened in common carp eggs, especially at 1 h postfertilization. The surface of fertilized adhesive egg of common carp was covered by a dense adhesive layer which gradually grew to be thickened after fertilization. While grass carp semi-buoyant eggs did not possess an adhesive layer after fertilization and the surface of grass carp semi-buoyant egg was smoother. Moreover, proteomic changes of egg envelopes between common carp adhesive eggs and grass carp semi-buoyant eggs at 1 h postfertilization showed that a rapid fold change of cytoskeleton-associated proteins (including actin, filamin-A, α-actinin, cytoskeleton-associated protein 5, and ezrin) was observed in common carp egg envelope after fertilization, which might be conductive to maintaining cell morphology and fish egg morphology. Significant upregulation of proteins such as a coagulation factor XIII A protein, zona pellucida proteins and cystatin in the fertilized egg of common carp might be responsible for inducing the process of egg envelope hardening in order to the protection of embryonic development. Obviously up-regulated level of alpha-2-macroglobulin-like protein in the fertilized egg of common carp could reflect the potentials of the effective immune defense in fertilized adhesive eggs of common carp. In conclusion, this study is the first to present the ultrastructure and protein composition in the egg envelope of common carp adhesive eggs and grass carp semi-buoyant eggs. Extensive changes in the abundance of proteins of egg envelope would throw some new lights on the biochemical processes underlying the early developmental processes after fertilization.

Respon Penetasan Telur Ikan Mas (Cyprinus carpio) pada Tingkatan Suhu yang Berbeda

ABSTRACT: The present study aimed to determine the effect of different temperature levels on the hatching rate of common carp eggs. This study consisted of 12 experimental units, in which 4 treatments with 3 replications were applied, namely A (control temperature 22-29 ° C), B (24 ° C), C (temperature 28 ° C), and D (temperature 32 ° C). Temperature, pH, DO and hatching rate was measured for data collection. Analysis of variance (ANOVA) with a confidence level of 95% was performed for statistical analysis. Water quality data were descriptively described. The results showed that the highest number of hatching rate was obtained in treatment D (32 ° C) of 290 (99%), C (28 ° C) of 276 (94%), treatment B (24 ° C) of 251 individuals, and the lowest was observed in treatment A (22-29 ° C) of 242 individuals (83%). Statistically, the temperature of 22-32 ° C did not affect the hatching rate of common carp eggs.
 ABSTRAK: Penelitian ini bertujuan untuk mengetahui pengaruh tingkatan suhu yang berbeda terhadap daya tetas telur ikan mas. Penelitian ini terdiri dari 12 satuan percobaan, yang mana terdapat 4 perlakuan dengan 3 ulangan yakni A (kontrol suhu 22-29°C), B (24°C), C (suhu 28°C) dan D (suhu 32°C). Parameter uji meliputi suhu, pH, DO dan daya tetas telur (hatching rate). Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) dengan tingkat kepercayaan 95% dan jika menunjukkan pengaruh nyata akan dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Data kualitas air dijelaskan secara deskriptif sesuai kelayakan hidup ikan mas. Hasil penelitian menunjukkan bahwa jumlah telur yang menetas terbanyak diperoleh pada perlakuan D (32°C) sebesar 290 ekor (99%), perlakuan C (28°C) sebesar 276 ekor (94%), perlakuan B (24°C) sebesar 251 ekor dan terendah pada perlakuan A (22-29°C) sebesar 242 ekor (83%). Hasil uji statistik menunjukkan bahwa suhu 22-32°C tidak berpengaruh terhadap daya tetas telur Ikan Mas.

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm

Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straws volumes and thawing rates on the post-thaw quality and fertilization ability of cryopreserved common carp ( Cyprinus carpio ) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl 2 , 1 mM MgSO 4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4°C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120ºC) and finally stored in liquid nitrogen (-196ºC) tank. Thawing of cryopreserved semen process was performed at 30ºC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30ºC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

The effects of ciprofloxacin on early life stages of common carp (Cyprinus carpio).

The authors performed a toxicity test with ciprofloxacin in fertilized eggs of common carp according to guideline 210 of the Organisation for Economic Co-operation and Development. The tested concentrations were 1 μg L(-1) , 100 μg L(-1) , 500 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) . Accelerated hatching was found in all groups exposed to ciprofloxacin, but significant growth reduction was found only in the group exposed to the highest concentration (3000 μg L(-1) ). Increased numbers of macroscopic morphological anomalies were observed on day 6 of the test (after hatching). The highest numbers of macroscopic morphological anomalies were observed in the groups of free embryos and larvae exposed to ciprofloxacin concentrations of 100 μg L(-1) , 500 μg L(-1) , 1000 μg L(-1) , and 3000 μg L(-1) (20-23% of tested samples). A gradual decrease in glutathione S-transferase activity was detected in all experimental groups exposed to ciprofloxacin, but significant differences (p < 0.01) were found only in groups treated with 500 μg L(-1) and 3000 μg L(-1) . Glutathione peroxidase and catalase exhibited increased activity in most of the tested concentrations (p < 0.01 and <0.05, respectively), whereas decreased glutathione reductase activity was found in the groups exposed to ciprofloxacin concentrations of 500 μg L(-1) and 3000 μg L(-1) (p < 0.05). The concentration of thiobarbituric acid-reactive substances was significantly lower (p < 0.01) in all experimental groups exposed to ciprofloxacin. The lowest-observed-effect concentration of ciprofloxacin was 1 μg L(-1) . These results suggest that hatching, early ontogeny, occurrence of morphological anomalies, antioxidant and biotransformation enzyme activity, and lipid peroxidation in fish can be affected by ciprofloxacin. Environ Toxicol Chem 2016;35:1733-1740. © 2015 SETAC.

The effect of osmolality on egg fertilization in common carp, Cyprinus carpio Linnaeus, 1758

The aim of the study was to investigate the effect of osmolality of the activating solution (AS) on in vitro fertilization of the eggs of common carp, Cyprinus carpio L. Four different AS (NaCl solutions) were used with different osmolalities: AS-0 (distilled water), AS-100 (100 mOsm kg−1), AS-200 (200 mOsm kg−1) and AS-300 (300 mOsm kg−1). Fertilization was performed in Petri dishes using freshly collected sperm. Insemination (sperm addition to activated eggs) was preceded by exposure of egg samples to each AS for 0 (control group), 15, 30, 60, 90, 120, 150, 180, 210, 240, 270, 300, 330 and 360 s. Embryonic survival was determined 24 h after incubation at 20°C. Additionally, motility parameters (motility rate [MOT, %] and curvilinear velocity [VCL, μm s−1]) of the spermatozoa activated with the tested AS were measured using a CASA (computer-assisted sperm analysis) system. Motility duration (DUR, s) in each AS was also determined. The results revealed a significant effect of osmolality of the AS on the fertilizing capacity of common carp eggs. There were no viable embryos in the group AS-300. In the group AS-0, the eggs lost their ability for fertilization after 90 s; in the remaining groups, eggs could be fertilized for up to 150 s (group AS-100) or 360 s (group AS-200). Fertilization failure in the group AS-300 was caused by the lack of spermatozoa activation in this solution. The present study findings for in vitro common carp fertilization indicate that special attention should be given to the AS osmolality, where 100 mOsm kg−1 was found to be the most suitable.

English

The study investigated the growth of hydromycoflora on the eggs of common carp (Cyprinus carpio L.) in five water bodies of different eutrophication levels. Thirty-three (33) species of mycotal organisms, including 28 species belonging to Peronosporomycota, 2 to anamorphic fungi and 1 each to Ascomycota, Blastocladiomycota and Zygomycota were identified on the eggs. The water from River Biala and Pond Fosa, which are more biogenic, had the largest number of mycotal species (20 and 19, respectively) on the eggs of common carp. The smallest number of those species was found on the eggs of common carp in water from Spring Cypisek, Pond Komosa and River Suprasl, which are low in biogenes (12, 11 and 11, respectively). Achlya diffusa, Aphanomyces laevis, Saprolegnia ferax, Saprolegnia parasitica and Pythium ultimum belong to the species that were most frequently found on C. carpio eggs. The following rare mycotal species were also found: Allomyces arbuscula, Aphanomyces frigidophilus, Candida albicans, Fusarium aquaeductum, F. culmorum and Zoopage phanera. Amino acid, carbohydrate and urease tests were used. Of the C. carpio eggs that were investigated, 12.7% were found to be infected with mycotal species. Key words: Cyprinus carpio, common carp, eggs, mycotal species, infections, hydrochemistry.

Egg stickiness in artificial reproduction of sturgeon: an overview

AbstractThe egg structure of sturgeon is complex, with multiple micropyles and an envelope structure different from that of teleosts and other fish groups. In the sturgeon, an adhesive layer in the follicular epithelium is responsible for the egg adhesiveness. This is a problem for artificial reproduction of many fish species, including sturgeon, when a large number of eggs are incubated in hatchery conditions. Although several techniques for removing adhesiveness have been developed and successfully applied to eggs of common carp, tench, pikeperch and European catfish, de‐sticking methods for sturgeon are limited. Proteolytic enzymes have been successfully applied to common carp, tench and African catfish eggs, leading to a hatching rate over 80% following 2‐min treatment. In sturgeon, blue clay is the most commonly used de‐sticking substance. The chemical reactions determining egg adhesiveness in sturgeon are poorly studied, and optimum methods for removing stickiness remain to be developed.

Induced Artificial Androgenesis in Common Tench, Tinca Tinca (L.), Using Common Carp and Common Bream Eggs

This study presents artificial induction using tench eggs, Tinca tinca (L.), of androgenetic origin. The oocytes taken from common bream, Abramis brama (L.) and common carp, Cyprinus carpio L. were genetically inactivated using UV irradiation and then inseminated using tench spermatozoa. Androgenetic origin (haploid or diploid embryos) was checked using a recessive colour (blond) and morphological markers. The percentage of hatched embryos in all experimental groups was much lower than in the control groups. All haploid embryos showed morphological abnormalities, which were recorded as haploid syndrome (stunted body, poorly formed retina, etc.). The optimal dose of UV irradiation of common bream and common carp eggs was 3456 J m–2. At this dose, almost 100% of haploid embryos were produced at a hatching rate of over 6%. Lower UV-ray doses affected abnormal embryo development. The highest yield of tench androgenesis (about 2%) was noted when eggs were exposed to thermal shock 30 min after egg activation.

The Effect of Polychlorinated Biphenyls Mixture (Aroclor 1254) on the Embryonic Development and Hatching of Prussian Carp, &lt;I&gt;Carassius Gibelio&lt;/I&gt;, and Common Carp, &lt;I&gt;Cyprinus Carpio&lt;/I&gt; (Actinopterygii: Cypriniformes: Cyprinidae)

Background. Polychlorinated biphenyls (PCBs) are persistent and bioacumulative chemical pollutants which exert negative physiological effects on the reproductive system of mature male and female fish. PCBs present in the aquatic environment may also have an influence on developing embryos. The aim of this study was to investigate the effect of Aroclor 1254, a polychlorinated biphenyls mixture, on the embryonic development of Prussian carp, Carassius gibelio (Bloch, 1782), and common carp, Cyprinus carpio L. Materials and methods. The samples of eggs obtained from 4 females of each species were divided into two dishes and incubated separately after fertilization (common carp) or activation (Prussian carp) with common carp sperm. The incubation, with Aroclor 1254 (1 or 10 ng · mL–1) or in water with no PCB added, lasted for 4 days. The mortality, hatching rate, number of hatched larvae, and number of deformed larvae were observed. Results. After 24 h of incubation of Prussian carp and common carp eggs there were no significant differences in the percentage of living eggs between the Aroclor 1254 treated groups and the control one. The lowest tested concentration of PCB (1 ng · mL–1) accelerated the hatching of Prussian carp larvae at 75 h of incubation. The significant increase in the percentage of deformed larvae was observed only in the experiment with common carp eggs incubated with Aroclor 1254 at the concentration of 10 ng · mL–1. Conclusion. Results of the presented data showed that Aroclor 1254 (at tested concentrations) is not harmful for the development of activated Prussian carp eggs but teratogenic effect was observed in the case of common carp embryos.

Development of Denuded Eggs of Common Carp

Development of Denuded Eggs of Common Carp

Physiological and biochemical investigations on egg stickiness in common carp

The properties and behaviour of common carp, Cyprinus carpio, eggs in water and in ovarian fluids were studied at different temperature, pH, and with divalent cation concentrations. The biochemical composition of zona radiata externa (ZRE) was analyzed qualitatively and quantitatively on amino acids, carbohydrates, uronic acid and sialic acids using chemical assays; on proteins using electrophoresis. Comparative biochemical studies were performed on the chub, Leuciscus cephalus, the vimba, Vimba vimba and the bleak, Chalcalburnus chalcoides. Eggs of common carp became sticky within seconds after mixing with water. Egg stickiness was not affected by water pH in a range of 6–9, by water temperatures between 4 and 30 °C, by divalent cations in concentrations ≤20 mmol/l, and by sodium chloride concentrations ≤50 mmol/l. Our investigations indicated that specific proteins of the cyprinid ovarian fluid are controlling (inhibiting) the initiation of egg stickiness: egg stickiness did not develop as long as the eggs were incubated in ovarian fluid. When however the ovarian fluid proteins were removed from the ovarian fluid by heat treatment, eggs developed stickiness within seconds, like they do in water. Biochemically, the ZRE consisted of nine types of proteins whereby four of them were glycoproteins. Glucose, fructose, galactose, and uronic acids were the major carbohydrates. Treatment of the egg membrane with invertase or amyloglucosidase did not affect the egg stickiness. Treatment with protease prevented stickiness. From these results and from additional histochemical results, we conclude that glycoproteins are likely to be the molecules responsible for stickiness.

PERBANDINGAN PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN MAS (Cyprinus carpio Linn.) DIPLOID DAN TETRAPLOID

The aim of this study were to know growth and gonadal development of tetraploidy common carp. The method that used in this study was experiment. Treatment that used was tetraploidyzation by heat shock 40°C during 1.5 minutes of common carp eggs to 29 minutes after fertilization. Five replicates were carried out for treatment. Parameters test were relative length growth (h), specific growth rate (SGR) and gonadal development of common carp. Data analysis that used was descriptive analysis. The result of this study indicated that tetraploidization teratment influenced on growth and gonadal development of common carp. The relative length growth of tetraploidy common carp was 5.38 ± 0.12 (30-days) and 19.12 ± 0.00 (110-days), as their specific growth rate was 19.87 ± 0.10 percent BW/day (30-days) and 8.57 ± 0.00 percent BW/day (110-days). Tetraploidy common carp have fertile characteristic and show gonadal development that same with diploid common carp relatively.

PERBANDINGAN PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN MAS (Cyprinus carpio Linn.) DIPLOID DAN TETRAPLOID

The aim of this study were to know growth and gonadal development of tetraploidy common carp. The method that used in this study was experiment. Treatment that used was tetraploidyzation by heat shock 40°C during 1.5 minutes of common carp eggs to 29 minutes after fertilization. Five replicates were carried out for treatment. Parameters test were relative length growth (h), specific growth rate (SGR) and gonadal development of common carp. Data analysis that used was descriptive analysis. The result of this study indicated that tetraploidization teratment influenced on growth and gonadal development of common carp. The relative length growth of tetraploidy common carp was 5.38 ± 0.12 (30-days) and 19.12 ± 0.00 (110-days), as their specific growth rate was 19.87 ± 0.10% BW/day (30-days) and 8.57 ± 0.00% BW/day (110-days). Tetraploidy common carp have fertile characteristic and show gonadal development that same with diploid common carp relatively.

PERBEDAAN KEBERHASILAN TINGKAT POLIPLOIDISASI IKAN MAS (Cyprinus carpio Linn.) MELALUI KEJUTAN PANAS

The easiest, cheapest, simpliest and efficient polyploidization method was heat shock treatment. Each fish species has different tolerance either initial time, temperature or duration of heat shock. The aim of this study were to show and determine the heat shock effects on differentiation of hatching rate, abnormality, survival rate, and successful polyploidization of common carp. The method that used in this study was experiment. Treatment that used was heat shock 40° C during 1.5 minutes of common carp eggs to 3 and 29 minutes after fertilization. Ten replicates were carried out for each treatment. Parameters test were hatching rate, abnormality, survival rate and polyploidy induction by counting of nucleolus number. Data analysis that used was descriptive. The result of this study indicated that heat shock treatment influenced on hatching rate, abnormality, survival rate and polyploidy induction of the common carp polyploidization. Hatching rate of common carp, triploidy and tetraploidy were 22.63 ± 8.36 percent and 11.10 ± 8.60 percent, abnormality 13.81 ± 4.67 percent and 24.86 ± 8.37 percent, survival rate 52.64 ± 8.46 percent and 55.04 ± 8.15 percent and polyploidy induction 70 ± 7.07 percent and 60 ± 7.07 percent, respectively.

Lipid Profile and Fatty Acid Composition in Eggs of Common carp (Cyprinus carpio)

The lipid class and fatty acid composition in eggs of Indian fresh water common carp Cyprinus carpio (Var. Cummunis Linnaeus) have been studied. The mean wet weight of mature eggs are 4.1% of total weight of fish, of which 9.8% (on dry basis) is lipid. The major portion of the lipid is phospholipid (PL), about 64.3%, of which phosphatidylcholine (PC) is predominant. The fatty acid composition of the total lipid, triacylglycerol (TAG) and phospholipids are observed. Palmitic acid (16:0) is the most abundant saturated fatty acids present in the total lipid (24.2%), TAG (33.2%) and PL (27.4%) fractions. Polyunsaturated fatty acids (PUFA) are more accumulated in the PL fraction than in the TAG. Arachidonic acid (20:4 n-6, AA) and docosahexaenoic acid (22:6 n-3, DHA) in the PL fraction are 13.0% and 15.2% and in TAG fraction 0.6% and 1.0%, respectively. The results of the study indicate that C. carpio eggs are rich in PUFAs which are very much essential in terms of fertilization success and larval development.

Thrombin induces assembly in vitro of vitelline envelope components of common carp eggs

Thrombin induces assembly in vitro of vitelline envelope components of common carp eggs

Hatching out goldfish from common carp eggs: interspecific androgenesis between two cyprinid species

We have successfully performed interspecific androgenesis between two cyprinid species. Gamma-ray irradiated eggs of common carp were fertilized with fresh and cryopreserved sperm of three different goldfish varieties and the haploid embryos were then heat-shocked to restore diploidy and to produce viable offspring. Androgenic diploid goldfish progenies from over a dozen different experiments were screened for four phenotypic markers several times. Color and other phenotypic markers characteristic of goldfish were found exclusively among androgenetic goldfish progenies; no markers originating from common carp were detected in over 1500 individuals investigated visually.RAPD assay was used to compare the parents and the offspring at the genomic level. The RAPD pattern of the androgenetic goldfish contained exclusively paternal bands, thereby confirming the results of the phenotypic analysis. According to our knowledge, this is the first successful interspecific androgenesis performed with two different species resulting in viable offspring.Key words: RAPD, whole genome manipulation, nuclear-mitochondrial incompatibility.