Commercial Utilization Research Articles

Load-and-go molecular diagnostics for human papilloma virus detection

The present study describes analytical and clinical performance of the HPV 16 & 18 assay for the detection of HPV specific DNA with near point-of-care utility. A room temperature compatible real time PCR test was developed for detection and discrimination of HPV 16/18 in a single tube. The test was integrated and validated on Compact series automated platform for analytical performance (sensitivity, specificity, accuracy). The clinical evaluation was performed on cervico-vaginal specimens. The analytical sensitivity data showed dynamic range from 50 IU/mL to 1´106 IU/mL using NIBSC international standard (19/224 for HPV16 and HPV18). The test was found to be highly specific for both the HPV targets with no cross reactivity against other HPV genotypes (high and low risk) and similar pathogens. The clinical performance on cervical sampling indicated up to 100% agreement with a comparator test method. The HPV 16 and 18 detection assay employing an integrated automated system offers low to high-throughput analysis with a cost-effective, pipette-free approach, eliminating the need for molecular infrastructure and technical expertise. This test named “HPV 16 and 18 detection kit” has been approved by Central Drugs Standard Control Organisation (India) for commercial utility, and would have significant implications for mass screening of cervical cancer.

Unlocking traditional remedy: Gulkand-enhanced mucoadhesive gel for canker sore relief

This study focuses on the development and evaluation of mucoadhesive gel formulations containing different Gulkand and its extract with a specific emphasis on their potential for managing oral health conditions, including the discomforting issue of mouth ulcers. The formulations underwent a thorough analysis, encompassing the assessment of phenolic compounds responsible for antioxidant activity, in addition to comprehensive physical, chemical, and pharmacological evaluations to determine their suitability for commercial utilization. Mouth ulcers are a prevalent oral health concern that can cause significant discomfort and inconvenience. In this study, Gulkand extract exhibited remarkable characteristics with its high phenolic content and robust antioxidant activity. It demonstrated rapid drug release, positioning it as a promising new for addressing the immediate relief needs of individuals suffering from mouth ulcers. Conversely, gel formulation showcased a sustained release profile suggesting the potential for longer therapeutic benefits, presents an intriguing option for oral health applications, capitalizing on the antiulcer properties associated with liquorice. An innovative aspect of this study is the compatibility assessment, which employed antioxidant and phenolic content analysis to verify the harmonious interaction between herbal constituents and excipients. This approach introduces novel perspective on compatibility testing, particularly critical for formulations designed to alleviate oral discomfort effectively. Furthermore, stability studies are warranted to be evaluated prior to their potential for commercialization. The present study revealed the promising potential of the prepared mucoadhesive gels in managing not only mouth ulcers but also a spectrum of oral health conditions, holding broader implications for advancements in the field of medicine.

Spatial Coordination Analysis and Development Methods of the Catering Sector in Yongkang City

The catering sector is recognized as a crucial driver for stimulating domestic consumption and fostering sustained economic growth. A scientifically informed spatial planning of the catering sector in county-level cities can help mitigate the overconcentration or excessive dispersal of resources, thereby enhancing the efficiency of land and commercial resource utilization, reducing waste, and promoting sustainable development within a county’s economy. This study focuses on Yongkang City, located in central Zhejiang Province, as a case study. Using Python 3.1 software to extract point-of-interest (POI) data, spatial analysis techniques such as standard deviation ellipses and kernel density estimation are employed to elucidate the spatial distribution characteristics of the catering sector, comprehensive transportation, and public services in Yongkang City. Furthermore, a coupling coordination degree model is utilized to quantify the coupling coordination degrees between the catering sector and comprehensive transportation (D1), as well as between the catering sector and public services (D2), ultimately assessing the overall coupling coordination degree among the three sectors (D3). Based on the analysis results, the coupling coordination degrees are categorized into five levels, followed by a detailed evaluation of the catering sector’s development across different subdistricts. The findings indicate a certain degree of spatial imbalance in the distribution of the catering sector in Yongkang City, with the western region exhibiting a more favorable development trajectory. Notably, Dongcheng Subdistrict achieved the highest coupling coordination degree (0.993), while the northern region, hindered by underdeveloped economic conditions and limited transportation infrastructure, exhibited the lowest coordination degree (0.098). Considering the unique developmental characteristics and current conditions of each subdistrict, this study advocates for the formulation of a long-term sustainable development plan for the catering sector in Yongkang City. The findings of this research may offer valuable insights for other regions and countries, thereby promoting the sustainable advancement of the catering sector.

Probiotic Characteristics and the Anti-Inflammatory Effects of Lactiplantibacillus plantarum Z22 Isolated from Naturally Fermented Vegetables.

Currently, there is increasing interest in the commercial utilization of probiotics isolated from traditional fermented food products. Therefore, this study aimed to investigate the probiotic potential of Lactiplantibacillus plantarum (L. plantarum) Z22 isolated from naturally fermented mustard. The results suggest that L. plantarum Z22 exhibits good adhesion ability, antibacterial activity, safety, and tolerance to acidic conditions and bile salts. We further determined the anti-inflammatory mechanism and properties of L. plantarum Z22 and found that L. plantarum Z22 could significantly reduce the secretion of pro-inflammatory cytokines, including interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and the expression of the pro-inflammatory mediator cyclooxygenase-2 (COX-2) protein in LPS-induced RAW 264.7 cells. In addition, L. plantarum Z22 also effectively inhibited the signaling pathways of nuclear factor κB (NF-κB) and mitogen-activated protein kinases (MAPKs). This effect can be attributed to a decrease in the levels of reactive oxygen species (ROS) and increased heme oxygenase-1 (HO-1) expression. Moreover, whole-genome sequencing revealed that L. plantarum Z22 contains gene-encoding proteins with anti-inflammatory functions, such as beta-glucosidase (BGL) and pyruvate kinase (PK), as well as antioxidant functions, including thioredoxin reductase (TrxR), tyrosine-protein phosphatase, and ATP-dependent intracellular proteases ClpP. In summary, these results indicated that L. plantarum Z22 can serve as a potential candidate probiotic for use in fermented foods such as yogurt (starter cultures), providing a promising strategy for the development of functional foods to prevent chronic diseases.

Bambusa vulgaris blooms along the Eastern plains of Uttar Pradesh

Bambusa vulgaris belonging to the subfamily Bambusoideae of Poaceae is the most widely grown bamboo along the tropics (Banik, 2000). Though described in early 1800, is rare in natural forest but evenly found in cultivation. In Uttar Pradesh it is one of the common bamboo along with Bambusa bambos and Dendrocalamus strictus planted on community lands. Its suitability to different agro-climatic conditions, culm endurance, commercial utility, high pulping quality, easy response to vegetative propagation, robust growth, quick recovery of clumps after felling and rare blooming are some of the significant features which make this a priority species. On reviewing the literature it was observed that this species has not been reported under gregarious flowering till date. Incidences of flowering in the species is very rarely reported along with absence of seed set (fruit) which is eventually due to certain physical and physiological factors. In the Chilbila market of the Pratapgarh district, one out of eight clumps was found under flowering in December 2022 in a homestead garden. Upon investigation, it was found that the clump has been in a flowering state for the past two years. However, only a few culms possess flowers that were leafless and yellow, while others without flowers were in normal growth. The emergence of new shoots was also observed in clumps. No seed setting was observed during the present flowering period.

Preparation of MAPbX3 perovskite thin film materials using in-situ doping atomized deposition methods

Thin films of organic halogenated perovskite MAPbX3 (X = Cl, Br, I or mixed, CH3NH3 = MA) display exceptional optical and electronic properties, rendering them highly promising materials for next-generation optoelectronics. However, achieving a large, thin, and smooth film poses a considerable challenge. Compared with the traditional spraying method, the atomized deposition method is able to break down the precursor solution molecules in the atomization system, and this method produces films with thinner thicknesses(800–1000 nm), smoother surfaces, high light transmittance and low surface roughness. By investigating the deposition time, annealing temperature, and film stability associated with the atomized deposition technique, we determined the optimal process parameters for producing nanometer-thick perovskite films. Specifically, we set the precursor solution concentration to 0.1 mmol/L, the deposition time to 450 s, added PVDF (polyvinylidene fluoride) colloid at a concentration of 3 wt%, and maintained an annealing temperature of 80 °C for 20 min. Ultimately, perovskite films with tunable bandgap were prepared for anti-counterfeit marking applications. This method of preparing perovskite films represents a significant advancement towards their commercial utilization in the realm of optoelectronic materials.

APPLICATION OF GOAL PROGRAMMING APPROACH FOR RUBBER MADE FURNITURE SPECIALLY WOOD DOOR MANUFACTURING FACTORY IN TRIPURA: A COMPARATIVE STUDY

Rubber plantation requires re-planting after 31-35 years when latex collection becomes uneconomical. For commercial utilization and value addition to rubber logs felled during re-plantation, processing of Rubber Timber is vital otherwise, there is no timber value. Restriction on the use of forest timber inspired the establishment of the Rubber Wood manufacturing unit. The carpentry unit plays an important role in popularizing the use of eco-friendly Rubber wood in the country. Making of doors from solid rubber wood and treated timber has good market in the country as plenty of houses are being built in the country. Additionally, Rubber Wood Door has enormous export potential. Sen and Nandi (2012) developed a mathematical model in their paper. In this paper, I consider four cases that change the basic structure of the model, and a comparative study is conducted.

Rh–Ni(OH)2/NF via hydrolysis galvanic replacement of boride: Unveiling an exceptional electrocatalyst for high-current alkaline hydrogen evolution

The efficient and high-quality production for hydrogen through water electrolysis at high current densities is crucial for commercial utilization. However, the performance of existing hydrogen evolution reaction (HER) electrocatalysts is far from satisfactory. In this regard, we proposed a method to prepare Rh–Ni(OH)2 catalyst based on Nickel foam (NF). The hydrolysis galvanic replacement of nickel boride with RhCl3 led to produce a lattice contraction in Ni(OH)2 due to the rigid pressure generated, resulting in outstanding HER performance at high current densities. It achieves an industrial current density of 500 mA cm−2 with an overpotential of 130 mV. Furthermore, at the industrially prefer temperature of 80 °C, only 67 mV overpotential is required. The catalyst also demonstrated exceptional stability, maintaining excellent performance even after a stability test of 100 days with a current density of 200 mA cm−2. Through Density-functional theory (DFT) calculations, Rh series reduced the hydrogen adsorption free energy.

Unlocking the Therapeutic Potential of Medicinal Hemp: Insights into Pharmacokinetics and Metabolism of Cannabinoids

Abstract: Medicinal hemp or Cannabis (Cannabis sativa L.) is one of the earliest herbs widely used in traditional medicine by human beings for millions of years. This species was originally thought to be endemic to Asian countries but has now grown worldwide. It is a dioecious and annual plant that belongs to the Cannabaceae family. It has a long history of cultivation for food, intoxicants, hemp medicine, rope, paper, fiber, and oil extraction. Due to its diverse therapeutic properties, it is legalized in over 50 countries and cultivated as a medicinal and recreational crop. This review was aimed at collecting information about medicinal cannabis, ranging from traditional use to commercial utilization. This review article is based on gathering scientific literature from Google Scholar, Scopus, PubMed, Science Direct, Wiley Online Library, Taylor Francis, and Springer with 119 documents, including original articles, review articles, books, and thesis from 1973 to 2024. So far, more than 500 phytochemicals have been identified. Phytocannabinoids are thought to be the main bioactive ingredients that produce therapeutic benefits. These are mostly produced or synthesized in secretary cells located inside of glandular trichomes that are found in the female inflorescence or flowers of hemp crop. Cannabidiol (CBD) has strong therapeutic potential and is used to cure many fatal diseases. A thorough understanding of the pharmacokinetics and metabolism of medicinal hemp is vital for tailoring treatment approaches and minimizing adverse effects. The effect and severity of Cannabidiol (CBD) and tetrahydrocannabinol (THC) are determined by the route of application and their dose. It can be concluded that much of the work needs to be done for the validation of ethnobotanical claims by conducting detailed studies on exploring various phytocannabinoids regarding medicinal use through in vitro and in vivo activities and the way forward for its commercial utilization.

Extraction of polyphenols from Apocynum venetum leaves using customized deep eutectic solvents: Process optimization and antioxidant evaluation

To encourage the commercial utilization of A. venetum polyphenols as bio-colorant on textile, five different combinations of deep eutectic solvents (DESs) were evaluated using a single-factor approach and the extraction of polyphenols from A. venetum leaves by ultrasound-assisted DESs was optimized. At the same time, the antioxidant activity of polyphenols prepared were investigated. The DESs composed of choline chloride and glycerol with a molar ratio of 1:2 and a water content of 20 % showed higher extraction performance than other DESs and traditional solvents (water, 60 % ethanol and 60 % methanol). With the most effective solvent the maximum yield 8.24 % of polyphenols was obtained under the optimal extraction conditions were as follows: the ratio of liquid volume (mL) to solid mass (g) is 49:1, extraction temperature of 77 ℃, and extraction time of 55 min. In addition, the polyphenols that have been separated and purified from the DESs extract exhibited antioxidant activities in three kinds of assays including 2,2-diphenyl-1-picrylhydrazyl stable free radical (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid free radicals (ABTS) and hydroxyl radical (•OH) scavenging activity. Therefore, this paper demonstrates that DESs are effective and environmentally friendly solvents extraction of antioxidant polyphenols pigments from the leaves of A. venetum.

Glyphosate resistance and no fitness cost in backcross offspring of wild soybean and transgenic soybean with epsps gene

BackgroundThe commercial utilization of genetically modified soybeans has yielded substantial economic advantages. Nevertheless, the genetic drift towards wild soybeans is one of the main ecological risks that needs to be addressed. Previous experiments demonstrated the absence of fitness cost or florescence overlap in hybrid offspring resulting from the crossbreeding of transgenic soybean GTS40-3-2 and Zhengzhou wild soybeans. In this study, hybrid progeny was systematically crossed with wild soybeans to establish a backcross progeny system. This system was employed to evaluate the ecological risk associated with the backcross progeny of transgenic and wild soybeans.ResultsThe findings indicated that the offspring from the backcross exhibited glyphosate tolerance. Furthermore, the expression of foreign proteins in the backcross offspring was notably lower than in the transgenic soybean, and there was no significant difference when compared to the hybrid progeny. Parameters such as germination rate, aboveground biomass, pods per plant, full seeds per plant, and 100-grain weight exhibited no significant differences between the negative and positive lines of the backcross progenies, and no fitness cost was identified in comparison to wild soybeans. These results underscore the potential for foreign genes to propagate within other wild soybeans, which requires continuous attention.ConclusionsThe widespread adoption of genetically modified soybeans has undeniably led to substantial economic gains. However, the research findings emphasize the critical importance of addressing the ecological risks posed by genetic drift towards wild soybeans. The backcross progeny system established in this study indicates that the potential for foreign gene dissemination to wild soybean populations warrants continued attention and mitigation strategies.

Modelling and mapping the abundance of lingonberry (Vaccinium vitis-idaea L.) in Norway

Lingonberry (Vaccinium vitis-idaea L.) grows in a range of nature types in the boreal zone, and understanding factors affecting the abundance of the plant, as well as mapping its spatial distribution, is important. The abundance of the species can be an indicator of ecosystem changes, and lingonberry can also be a source for commercial utilisation of berry resources. Using country-wide data from 6404 field plots of the Norwegian national forest inventory (NFI), we modelled the relationship between lingonberry cover and airborne laser scanning (ALS) and satellite metrics and bioclimatic variables describing the forest structure, terrain, soil properties and climate using a generalised mixed-effects model with a quasipoisson distribution. The validation carried out with an independent set of 2124 NFI plots indicated no obvious bias in predictions. The most important predictors were found to be interactions between dominant tree species, stand basal area and latitude, as well as the reflectance in the near-infrared band from Sentinel-2 satellite imagery, the dominant height based on the ALS variable and the long-term mean summer (June–August) temperature. The results provide an indicator of the effects of global warming, as well as the possibility of giving forest management prescriptions that favour lingonberry and locating the most abundant lingonberry sites in Norwegian forests.

Structural color of metallic glass through picosecond laser

Abstract The alteration in surface color of metallic glasses (MGs) holds great significance in the context of microstructure design and commercial utility. It is essential to accurately describe the structures that are formed during the laser and color separation processes in order to develop practical laser coloring applications. Due to the high oxidation sensitivity of La-based metallic glass, it can broaden the color range but make it more complex. Structure coloring by laser processing on the surface of La-based metallic glass can be conducted after thermoplastic forming. It is particularly important to clarify the role of structure and composition in the surface coloring process. The aim is to study the relationship between amorphous surface structural color, surface geometry, and oxide formation by laser processing in metallic glasses. The findings revealed that the periodic structure primarily determines the surface color at laser energy densities below 1.0 J/mm2. In contrast, the surface color predominantly depends on the proportion of oxides that are formed when energy densities exceed 1.0 J/mm2. Consequently, this study provides a novel concept for the fundamental investigation of laser coloring and establishes a new avenue for practical application.

Activating Redox Kinetics of Li2S via Cu+, I- Co-Doping Toward High-Performance All-Solid-State Lithium Sulfide-Based Batteries.

All-solid-state lithium sulfide-based batteries (ASSLSBs) have drawn much attention due to their intrinsic safety and excellent performance in overcoming the polysulfide shuttle effect. However, the sluggish kinetics of Li2S cathode severely impede commercial utilization. Here, a Cu+, I- co-doping strategy is employed to activate the kinetics of Li2S to construct high-performance ASSLSBs. The electronic conductivity and Li-ion diffusion coefficient of the co-doped Li2S are increased by five and two orders of magnitude, respectively. Cu+ as a redox medium greatly improves the reaction kinetics, which is supported by ex situ X-ray photoelectron spectroscopy. Density functional theory calculation (DFT) shows that Cu+, I- co-doping reduces the Li-ions diffusion energy barrier. The co-doped Li2S exhibits a remarkable improvement in capacity (1165.23 mAh g-1 (6.65 times that of pristine Li2S) at 0.02 C and 592.75 mAh g-1 at 2 C), and excellent cycling stability (84.58% capacity retention after 6200 cycles at 2 C) at room temperature. Moreover, an ASSLSB, fabricated with a lithium-free (Si─C) anode, obtains a high specific capacity of 1082.7 mAh g-1 at 0.05 C and 97% capacity retention after 400 cycles at 0.5 C. This work provides a broad prospect for the development of ASSLSBs with practical energy density exceeding that of traditional lithium-ion batteries.

Inhibitory effects of potassium sorbate and ZnO nanoparticles on Escherichia coli and Staphylococcus aureus in milk-based beverage

Antibacterial substances have played a crucial role in food preservation. Herein, the inhibitory effects of potassium sorbate and ZnO nanoparticles (ZnO NPs) on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) in milk-based beverages were examined through the determination of their minimum inhibitory concentrations (MICs) with an automated method for measuring bacterial growth curves. We revealed that the MICs of potassium sorbate and ZnO NPs against E. coli were 16.0 mg/mL and 8.0 mg/mL, respectively. While against S. aureus they were 24.0 mg/mL and 8.0 mg/mL, respectively. ZnO NPs demonstrated higher antibacterial efficacy compared to potassium sorbate. The combination of potassium sorbate and ZnO NPs showed an indifferent effect on E. coli, but an additive effect on S. aureus. The addition of 3 × MIC of either potassium sorbate or ZnO NPs resulted in a complete suppression of bacterial growth for 5 days. This study underscored the efficacy of potassium sorbate and ZnO NPs, either alone or in combination, in combating E. coli and S. aureus, highlighting their potential commercial utility in safeguarding milk-based beverages from spoilage.

Ion‐sieving MXene flakes with quantum dots enable high plating capacity for dendrite‐free Zn anodes

AbstractThe commercial utilization of Zn metal anodes with high plating capacity is significantly hindered by the uncontrolled growth of dendrites and associated side reactions. Herein, a robust artificial ion‐sieving MXene flake (MXF)‐coating layer, with abundant polar terminated groups, is constructed to regulate the interfacial Zn2+ deposition behavior. In particular, the fragmented MXF coupled with in situ generated quantum dots not only has strong Zn affinity to homogenize electric fields but also generates numerous zincophilic sites to reduce nucleation energy, thus securing a uniform dendrite‐free surface. Additionally, the porous coating layer with polar groups allows the downward diffusion of Zn2+ to achieve bottom‐up deposition and repels the excessive free water and anions to prevent parasitic reactions. The ion‐sieving effect of MXF is firmly verified in symmetric cells with high areal capacity of 10–40 mAh cm−2 (1.0 mA cm−2) and depth of discharge of 15%–60%. Therefore, the functional MXF‐coated anode manifests long‐term cycling with 2700 h of stable plating/stripping in Zn||Zn cell. Such rational design of MXF protective layer breaks new ground in developing high plating capacity zinc anodes for practical applications.

A review on endophytic bacteria of orchids: functional roles toward synthesis of bioactive metabolites for plant growth promotion and disease biocontrol.

In this review, we have discussed the untapped potential of orchid endophytic bacteria as a valuable reservoir of bioactive metabolites, offering significant contributions to plant growth promotion and disease protection in the context of sustainable agriculture. Orchidaceae is one of the broadest and most diverse flowering plant families on Earth. Although the relationship between orchids and fungi is well documented, bacterial endophytes have recently gained attention for their roles in host development, vigor, and as sources of novel bioactive compounds. These endophytes establish mutualistic relationships with orchids, influencing plant growth, mineral solubilization, nitrogen fixation, and protection from environmental stress and phytopathogens. Current research on orchid-associated bacterial endophytes is limited, presenting significant opportunities to discover new species or genetic variants that improve host fitness and stress tolerance. The potential for extracting bioactive compounds from these bacteria is considerable, and optimization strategies for their sustainable production could significantly enhance their commercial utility. This review discusses the methods used in isolating and identifying endophytic bacteria from orchids, their diversity and significance in promoting orchid growth, and the production of bioactive compounds, with an emphasis on their potential applications in sustainable agriculture and other sectors.

Physicochemical Characterization of Oil and Chitosan Extracted from Cross Breed and Bivoltine Hybrid Silkworm Pupae: A Comparative Analysis

The oil and chitosan were extracted from silkworm pupae and analysed its physicochemical properties. Oil content in silkworm pupae ranged from 26 to 28 % on a dry weight basis. Notably, female pupae had a higher oil content (27.99 %) compared to males (27.03%). Further, bivoltine hybrids exhibited a slightly higher oil content (27.65 %) than cross breeds (27.30 %). In comparison to edible oils like groundnut oil and sunflower oil, the pupal oil showed favourable results in terms of moisture content, iodine value and saponification values, indicating quality similar to edible oils. However, acid value of silkworm pupal oil (1.570 mg KOH/g oil) was lower than that of groundnut oil (3.125 mg KOH/g oil). The acid value and peroxide value were found to be lower than three in pupal oil samples, revealed that pupal oil is good for edible purposes and indicates good oxidative stability. Among the samples, Chitosan content was higher 2.526% in the cross breed (PM × CSR2) than bivoltine hybrid 2.308%. Male silkworm pupae had a higher chitin content (3.242%) compared to female pupae (3.013%) and chitosan content was also higher (2.430%) in male pupae than in female pupae (2.345%). Pupal chitosan shows better solubility (99 %), degree of deacetylation (>85%) and ash content (<1%). These physicochemical properties were achieved the desired level for utilizing the silkworm pupal oil and chitosan as commercial utilization.

Reconditioning commercial vehicle booth from industrial bakery waste to support herbal products distribution in Menik Jaya Community Group

The Menik Jaya Community Group faces constraints in distributing herbal products, impacting sales quality and quantity. Long distances, limited transportation, and weather-related product damage pose significant challenges. Additionally, bakery industry fiberglass booth waste presents an environmental issue. This community service aimed to address these problems by: 1) Providing more effective distribution through two-wheeled commercial booths; 2) Recycling fiberglass booth waste into functional commercial booths; and 3) Enhancing the group's herbal product marketing capacity. Methods included socialization, booth-making training, and commercial booth utilization assistance. Results yielded environmentally friendly commercial booths, improved distribution efficiency, and increased community income.

AdNAC20 Regulates Lignin and Coumarin Biosynthesis in the Roots of Angelica dahurica var. Formosana.

Angelica dahurica var. formosana (ADF), which belongs to the Umbelliferae family, is one of the original plants of herbal raw material Angelicae Dahuricae Radix. ADF roots represent an enormous biomass resource convertible for disease treatment and bioproducts. But, early bolting of ADF resulted in lignification and a decrease in the coumarin content in the root, and roots lignification restricts its coumarin for commercial utility. Although there have been attempts to regulate the synthesis ratio of lignin and coumarin through biotechnology to increase the coumarin content in ADF and further enhance its commercial value, optimizing the biosynthesis of lignin and coumarin remains challenging. Based on gene expression analysis and phylogenetic tree profiling, AdNAC20 as the target for genetic engineering of lignin and coumarin biosynthesis in ADF was selected in this study. Early-bolting ADF had significantly greater degrees of root lignification and lower coumarin contents than that of the normal plants. In this study, overexpression of AdNAC20 gene plants were created using transgenic technology, while independent homozygous transgenic lines with precise site mutation of AdNAC20 were created using CRISPR/Cas9 technology. The overexpressing transgenic ADF plants showed a 9.28% decrease in total coumarin content and a significant 12.28% increase in lignin content, while knockout mutant plants showed a 16.3% increase in total coumarin content and a 33.48% decrease in lignin content. Furthermore, 29,671 differentially expressed genes (DEGs) were obtained by comparative transcriptomics of OE-NAC20, KO-NAC20, and WT of ADF. A schematic diagram of the gene network interacting with AdNAC20 during the early-bolting process of ADF was constructed by DEG analysis. AdNAC20 was predicted to directly regulate the transcription of several genes with SNBE-like motifs in their promoter, such as MYB46, C3H, and CCoAOMT. In this study, AdNAC20 was shown to play a dual pathway function that positively enhanced lignin formation but negatively controlled coumarin formation. And the heterologous expression of the AdNAC20 gene at Arabidopsis thaliana proved that the AdNAC20 gene also plays an important role in the process of bolting and flowering.