Commercial Scale Research Articles

The effects of temperature and CO2 enrichment on the red seaweed Asparagopsis taxiformis from Southern California with implications for aquaculture.

The red alga Asparagopsis taxiformis has recently been recognized for its unique ability to significantly reduce methane emissions from ruminant animals when fed in small quantities. The main obstacle in using this seaweed as a methane-mitigating feed supplement is the lack of commercially available biomass. Little is known about how best to grow this red alga on a commercial scale, as there are few published studies that have investigated the factors that influence growth, physiology, and overall performance. This study examined the effects of temperature and CO2 enrichment on the growth, photophysiology, and concentration of bromoform, the secondary metabolite largely responsible for methane reduction in A. taxiformis. A series of single and multifactor closed culture experiments were conducted on A. taxiformis collected, isolated, and cultured from populations in Southern California. We identified the optimal temperature range to be between 22 and 26°C, with significant short-term stress observed below 15°C and above 26°C. Carbon dioxide addition resulted in increased performance, when accounting for growth per CO2 use. In general, we observed the highest bromoform concentrations in algae with the highest growth rates, but these results varied among experiments. These findings indicate that through environmental control and by addressing limiting resources, significant increases in biomass production and quality can be achieved.

An Updated Review on Nanoemulsion: Factory for Food and Drug Delivery.

A nanoemulsion is a colloidal system of small droplets dispersed in another liquid. It has attracted considerable attention due to its unique properties and various applications. Throughout this review, we provide an overview of nanoemulsions and how they can be applied to various applications such as drug delivery, food applications, and pesticide formulations. This updated review aims to comprehensively overview nanoemulsions and their applications as a versatile platform for drug delivery, food applications, and pesticide formulations. Research relevant scientific literature across various databases, including PubMed, Scopus, and Web of Science. Suitable keywords for this purpose include "nanoemulsion," "drug delivery," and "food applications." Ensure the search criteria include recent publications to ensure current knowledge is included. Several benefits have been demonstrated in the delivery of drugs using nanoemulsions, including improved solubility, increased bioavailability, and controlled delivery. Nanoemulsions have improved some bioactive compounds in food applications, including vitamins and antioxidants. At the same time, pesticide formulations based on nanoemulsions have also improved solubility, shelf life, and effectiveness. The versatility of nanoemulsions makes them ideal for drug delivery, food, and pesticide formulation applications. These products are highly soluble, bioavailable, and targeted, providing significant advantages. More research and development are required to implement nanoemulsion-based products on a commercial scale.

A Comprehensive Review of Pfu DNA Polymerase: Extraction, Production and Applications

Pfu DNA Polymerase, a thermostable enzyme derived from the hyperthermophilic archaeon Pyrococcus furiosus, is widely recognized for its high fidelity and strong processivity. Its 3'-5' exonuclease activity makes it indispensable for the correct amplification of both short and complex DNA strands. These biochemical properties of Pfu DNA Polymerase have encouraged considerable advancements in its extraction and production methods. This review covers some of the traditional approaches for purification, including protein purification and affinity chromatography, and updates on recent advances in recombinant gene expression, automated systems of production, and membrane-based technologies. New ways of engineering enzymes have recently been developed, such as CRISPR-Cas9-mediated gene optimization, which raised the bar on extraction efficiency to meet emerging demand. Once challenging, Pfu DNA Polymerase production has been significantly streamlined through recombinant expression in E. coli both at a laboratory and commercial scale. The optimization techniques that are involved with IPTG concentration and Response Surface Methodology have increased the yield by up to 30%. Auto-induction means even higher biomass outputs are allowed. Today, applications of Pfu DNA Polymerase span from standard PCR up to advanced clinical diagnostics in the fields of molecular biology, forensic analysis, clinical microbiology, and biotechnology.

Exploration of the Biotechnological Potential of Two Newly Isolated Haematococcus Strains from Reunion Island for the Production of Natural Astaxanthin.

Haematococcus lacustris is a freshwater green microalgae species able to produce and accumulate astaxanthin in response to environmental stresses such as high light and nutrient deprivation. Astaxanthin is a xanthophyll carotenoid of growing economic interest due to its numerous biological activities, notably its strong antioxidant properties, which can be valued in the fields of nutrition, health, feed and aquaculture. The present study aims at evaluating the capacity of two newly isolated Haematococcus strains from the biodiversity of Reunion Island, to be cultivated in a photobioreactor and to produce astaxanthin. The results showed that both strains were able to grow in various nutritive media and to produce and accumulate astaxanthin in response to stresses, mainly in the form of astaxanthin monoesters, which represented up to 2% of the dry biomass weight and which were mostly composed of linoleic and linolenic acids. In fed-batch cultures using 3 L benchtop photobioreactors, the concentrations of biomass enriched in astaxanthin reached up to 3 g L-1 (dry weight) with biomass productivities of 0.04 and 0.02 g L-1 d-1 based on the durations of the vegetative stage and of the entire culture, respectively. In these cultures, the astaxanthin productivities were found to reach on average around 0.25 mg L-1 d-1. Although these results were relatively low compared to the literature, the possibility of improving growth conditions in order to improve biomass and astaxanthin yields, to guarantee economic viability for cultivation at a commercial scale, was further discussed.

Investigation of structural and electrical properties of electrolyte LaGaO3 for solid oxide fuel cell.

Electrochemical devices such as solid oxide fuel cells (SOFCs) allow the direct transformation of fuel's chemical energy into electrical power. Even though YSZ electrolyte-based conventional SOFCs are widely used in both laboratories and on a commercial scale, developing alternative ion-conducting electrolytes is crucial for enhancing SOFC performance at lower operating temperatures. In this work, we conducted a thorough computational analysis on the characteristics of Sr- and Mg-doped superior oxide ion conductors. We have used the DFT technique to examine the system's electrical and structural characteristics and the impact of doping. The GII value and LaGaO3 formation energy are used to investigate thermodynamical and structural stability, respectively. Theoretical investigations are validated against data to ensure the accuracy of the computational model. The research shows that the properties of Sr- and Mg-doped LaGaO3 have changed in a desirable way. This DFT study sheds light on the underlying mechanisms that affect the structural and electronic properties of LaGaO3 electrolytes and offers a thorough investigation of the synergistic effects of strontium and magnesium co-doping. The knowledge acquired is critical for the logical design and development of more stable and efficient solid oxide fuel cells.

A Comprehensive Review of Advanced Treatment Technologies for the Enhanced Reuse of Produced Water

Produced water (PW) is considered to be the largest source of industrial wastewater associated with oil and gas extraction operations for industrial production. It is a mixture of organic and inorganic compounds that has high complexity in terms of various characteristics. Globally, the volume of PW is increasing along with the expansion of gas and oil fields, leading to major impacts on the environment. Existing treatment technologies involve partially treating the PW through removing the suspended solids, heavy metals, without removing organic components and re-injecting the water underground using water disposal injection wells. The treatment process consists of a primary treatment unit to remove the particles, followed a secondary biological or chemical processing treatment, while the final treatment stage involves the use of a tertiary treatment unit to improve the water quality and remove the remainder of the undesired components. Moreover, while PW is considered one of the available options to be utilized as a water source, no alternate advanced treatment options on a commercial scale are available at present due to the limitations of existing PW treatment technologies, associated with their maintainability, sustainability, cost, and level of quality improvement. As such, research focused on finding an optimal treatment approach to improve the overall process continues to be conducted, with the aim of reusing the water instead of injecting it underground. This literature review discusses the latest advanced technologies for PW treatment aimed at reusing the full stream capacity of PW and eliminating the need for wastewater disposal via injection. It is concluded that researchers should focus on hybrid treatment technologies in order to remove the pollutants from PW, effectively allowing for its reuse.

6-Trifluoromethylnicotinamide Bilaterally Anchored All Cations Implementing Efficient and Humidity-Stable Perovskite Solar Cells.

For organic-inorganic hybrid perovskite solar cells (PSCs), the volatilization of organic components and the presence of undercoordinated Pb2+ ions are the primary causes of device stability imbalance. These factors also serve as significant determinants that restrict the commercialization scale of PSCs. Here, 6-trifluoromethylnicotinamide (TNA) molecules, featuring amide and trifluoromethyl groups at both ends, were introduced as Lewis additives into the precursor solution of perovskite to anchor all cation defect sites and optimize the crystallization process of perovskite. Theoretical calculations confirmed that the -NH2 and C═O groups within the amide group on one side of the TNA molecule synergistically passivated the undercoordinated Pb2+ ions, whereas the trifluoromethyl group on the other side formed hydrogen bonds with the organic components of perovskite. The scorpion-like bilateral all-cation anchoring ability of TNA molecules was further substantiated through experimental characterization. Upon optimization of the TNA treatment concentration, a perovskite film characterized by large grains and a reduced defect density of states was achieved. The photovoltaic performance of PSCs incorporating TNA exhibited significant enhancement with an increase in efficiency from 22.42% (Control) to 24.15%. Under a harsh high-humidity environment, the comprehensive cation passivation effect of TNA significantly hindered the decomposition and phase transition of perovskite films, thereby ensuring excellent humidity stability for the PSCs.

Interface engineering of three-phase NiO-CuO/Fe3O4 heterostructured catalyst for quick hydrogen release from ammonia borane methanolysis

Catalytic methanolysis of ammonia borane represents a promising technology for in situ hydrogen production. However, the widespread application of this technology is constrained by the prohibitive cost associated with noble metal catalysts. Consequently, the development of cost-effective and robust is essential for commercial scaling of this technology. In this study, a series of NiO-CuO/Fe3O4 catalyst with three-phase interfaces for ammonia borane methanolysis were fabricated by supporting NiO-CuO with Fe3O4. It was demonstrated that the Fe3O4 support in the catalyst could not only modulate the electronic structure of catalyst but also and confer magnetic characteristics upon it. The catalyst bearing a Ni/Cu molar ratio of 2:2 achieved a turnover frequency (TOF) of 13.5 min−1 in ammonia borane methanolysis, along with long-term stability. Furthermore, magnetic characterization confirmed the catalyst’s ferromagnetic properties with a coercivity of 51.8 Oe, facilitating the magnetic separation of the catalyst under an external magnetic field. Through various exhaustive characterizations, it was revealed that abundant interfacial positively charged Ni sites and negatively charged Cu sites in NiO-CuO/Fe3O4 catalyst could activate methanol and ammonia borane, respectively, thus promoting the hydrogen production efficiently. Considering the excellent activity and recyclability of NiO-CuO/Fe3O4, it is anticipated to gain widespread applications in the industrial production of hydrogen from ammonia borane methanolysis.

Innovative Z-scheme MnV₂O₆/g-C₃N₄ nanocomposite: photocatalytic, electrocatalytic and biosensing properties

Herein, we report Z-scheme mediated pn-junction between manganese vanadate and graphitic carbon nitride, highlighting its photocatalytic degradation capabilities, hydrogen and oxygen evolution reactions and electrochemical biosensing efficacy towards ascorbic acid. The material was synthesized using a co-precipitation method followed by annealing and characterized through XRD, FESEM, FTIR, XPS, UV–visible spectroscopy and EIS in conjunction with Mott-Schottky analysis. The heterojunction demonstrates promising results for photocatalytic degradation of organic dyes. This work further discusses the carrier concentration, decay rate, cyclic stability and photocatalytic degradation mechanism. The results for HER and OER indicate the synthesized material's capability for oxygen and hydrogen production at commercial scale, with Tafel slope calculations providing additional insights. Electrochemical biosensing of ascorbic acid reveals that the material can detect low concentrations of ascorbic acid under various parameters. The results indicate that the material is highly suitable for environmental degradation, electrocatalytic production of hydrogen and oxygen and medical diagnostics such as electrochemical biosensing.

Effect of Pre-Treatment on Bitterness and Quality of Osmodehydrated Lemons

Lemons are rich in beneficial phytochemicals and antioxidants, including phenolic compounds and flavonoids. However, some of these compounds impart a bitterness that can limit its application in food products and affect consumer acceptance. This study aimed to reduce the bitterness of osmodehydrated lemon using a combination of pre-treatment, osmotic dehydration, and drying processes. Fresh lemons, lemons immersed in 2 % NaCl for 3 nights, and lemons stored at room temperature for 3 days were analyzed for bitter compounds. These samples underwent 4 cycles of blanching at either 35 or 80 °C, followed by cooling, osmotic dehydration in a 60 °Brix sugar solution with heating at 80 °C for 45 min and soaking in syrup for an additional 30 min, and finally drying at 70 °C until reaching a water activity below 0.6. Results indicated that the optimal condition for reducing bitterness involved storing fresh lemons at room temperature for 3 days, soaking them in 2 % NaCl solution for 3 nights, and blanching at 80 °C, followed by osmotic dehydration and drying. The naringin, limonin, and hesperidin contents were reduced by 34-fold, 24-fold, and 10-fold, respectively, compared to those in fresh lemons. Additionally, its sensory properties were well accepted by panelists. Although the production cost at the laboratory scale was high, it is expected to be lower at pilot or commercial scales. The production method is deemed suitable for entrepreneurial ventures in the lemons processing industry. HIGHLIGHTS Value Addition: The study explored effective methods to value added and utilize lemon fruits by employing pre-treatment, blanching and cooling cycles, osmotic dehydration, and drying. Bitterness Reduction: The optimized production process significantly reduced the bitterness of the osmodehydrated lemon product. High Quality Process: The optimized production process resulted in high-quality osmodehydrated lemon products. Pre-Processing Storage: Storing fresh lemons at room temperature for 3 days before processing was effective in reducing bitterness. Consumer Approval: Panelists rated the osmodehydrated lemon favorably, indicating strong consumer preference. GRAPHICAL ABSTRACT

Salmonella Infantis Adhesion to Various Surfaces and In Vitro Antimicrobial Efficacy of Commercial Disinfectants.

Salmonella Infantis poses a significant challenge in poultry production due to its persistence and resistance to disinfectants. This study investigated the survival of the S. Infantis strain on different surfaces and evaluated the efficacy of disinfectants in both preventing and treating biofilms. The survival of the tested S. Infantis strain was assessed on plastic and stainless steel surfaces after 24 and 48 h. The minimum inhibitory concentrations (MICs) of five disinfectants were determined, and their antiadhesion effectiveness was evaluated using crystal violet. The efficacy of biofilm treatment was evaluated by cell culturability. The results showed that the adhesion of S. Infantis was significantly higher on the plastic surface. The disinfectants were effective at reducing biofilm formation only within the first 24 h. Fresh solutions of disinfectants based on quaternary ammonium compounds exhibited the highest antimicrobial efficacy, while chlorocresol was the most effective for both the prevention and treatment of biofilms. The study results suggest that the presence of plastic surfaces may contribute to the dissemination of Salmonella. Additionally, the effectiveness of disinfectants varied based on storage conditions and contact time, while biofilms demonstrated reduced susceptibility compared to planktonic cells. However, given the laboratory scale of this study, further validation on a commercial scale is necessary to confirm these findings.

Water Quality Analysis of a 300 Mvar Large-Scale Dual Internal Water Cooling Synchronous Condenser External Cooling System and Exploration of Optimal Water Treatment Agent Dosage at Different Temperatures

The external cooling water system of a 300 Mvar dual internal water cooling synchronous condenser at a certain ultra-high voltage converter station continued to exhibit significant scaling and corrosion, even with regular addition of scale and corrosion inhibitors. To solve this problem, the external cooling water of the synchronous condenser was sampled and tested periodically, with the main test items including conductivity, pH value, turbidity, hardness, alkalinity, and other water quality parameters directly related to corrosion and scaling. The trends of these parameters over time were also analyzed. The results showed that as the operation time increased, the cooling water became concentrated during multiple circulation cycles, and the various dissolved or suspended substances underwent a certain degree of enrichment. However, the addition of scale and corrosion inhibitors did not dynamically adjust according to the changes in water quality, and there was always an excessive dosage. Thus, using the external cooling water as the experimental sample, static scale inhibition tests and rotating coupon corrosion tests were conducted to evaluate the scale and corrosion inhibition performance of the commercial AS-582 scale and corrosion inhibitor used at this ultra-high voltage converter station under different conditions. Considering the more obvious corrosive tendency of this water sample, the focus was on testing its corrosion inhibition performance. When the dosage was 600 ppm, the scale inhibition effect was optimal, with an inhibition rate of 92.15%. The corrosion inhibition effect of this scale and the corrosion inhibitor were significantly related to water temperature. At 25 °C, when the dosage was 500 ppm, the corrosion inhibition effect was optimal, with an inhibition rate of 86.79%. However, when the temperature increased to 40 °C, the corrosion inhibition effect under each dosage was significantly worse, unable to meet the requirements, and the use of other corrosion inhibitors in combination was necessary. This work will provide a reference for the scientific use of scale and corrosion inhibitors.

Techno-economic analysis of green hydrogen integration into existing pipeline infrastructure: A case study of Wyoming

While green hydrogen production technologies like electrolysis have reached commercial scale, the primary challenge centers on efficient, and economically viable transportation of hydrogen. This study proposes an integrated geospatial techno-economic modeling approach to optimize hydrogen transportation through blending in existing pipelines. The simulations strategically locate green hydrogen production and blending points. The paper evaluates blending ratios, flow dynamics, and operating parameters to optimize hydrogen blending in natural gas pipelines. A techno-economic analysis quantifies costs and revenues across the supply chain, identifying the most economically viable hydrogen blending locations. The approach is validated through a case study in Wyoming, providing insights for industry stakeholders considering hydrogen-natural gas blending as a transition strategy. This integrated method offers a robust decision support tool for leveraging existing infrastructure to realize hydrogen's potential as a sustainable energy vector contributing to the broader goal of cleaner energy alternatives.

Data Hub for Life Cycle Assessment of Climate Change Solutions—Hydrogen Case Study

Life cycle assessment, which evaluates the complete life cycle of a product, is considered the standard methodological framework to evaluate the environmental performance of climate change solutions. However, significant challenges exist related to datasets used to quantify these environmental indicators. Although extensive research and commercial data on climate change technologies, pathways, and facilities exist, they are not readily available to practitioners of life cycle assessment in the right format and structure using an open platform. In this study, we propose a new open data hub platform for life cycle assessment, considering a hierarchical data flow starting with raw data collected on climate change technologies at laboratory, pilot, demonstration, or commercial scales to provide the information required for policy and decision-making. This platform makes data accessible at multiple levels for practitioners of life cycle assessment, while making data interoperable across platforms. The proposed data hub platform and workflow are explained through the polymer electrolyte membrane electrolysis hydrogen production as a case study. The climate change environment impact of 1.17 ± 0.03 kg CO2 eq./kg H2 was calculated for the case study. The current data hub platform is limited to evaluating environmental impacts; however, future additions of economic and social aspects are envisaged.

Optimization of autonomous vehicle control system reliability on a commercial scale through LIF dombi methodologies.

The resilient framework of Linguistic Intuitionistic Fuzzy Sets (LIFSs) allows for the representation and management of uncertainties related to intuitionistic judgments and linguistic expressions. Recent advances in passive and active safety systems have reduced highway fatalities. Autonomous vehicles can improve safety, efficiency, and mobility by navigating traffic without a driver. One of the primary benefits associated with this technology is that it reduces the number of traffic collisions that result in millions of fatalities and numerous injuries. In this research work, we devise two novel aggregation operators: the linguistic intuitionistic fuzzy Dombi ordered weighted averaging (LIFDOWA) operator and the linguistic intuitionistic fuzzy Dombi ordered weighted geometric (LIFDOWG) operator, and explore their fundamental structural properties. We provide innovative score and accuracy functions for multiple attribute decision-making (MADM) problems within the framework of LIF knowledge. Moreover, we use these techniques to develop a specialized algorithm for MADM issues that addresses the complexities arising from ambiguous data during the selection process. We also demonstrate the effectiveness of our proposed methods by applying them to solve the MADM scenario of selecting an optimal approach to improve the credibility of autonomous vehicle control systems on a commercial scale. In addition, we also compare and evaluate the authenticity and practicability of the newly designed techniques in comparison to existing methodologies.

Leveraging Spruce Bark Particle Morphology for Enhanced Internal Bonding in Particleboard Production.

The continuous rise in global demand for wood products has led to an increase in prices and a surge in research into alternative resources. As a byproduct of the timber industry, bark has emerged as a promising supplement in particleboard (PB) production. However, its anatomical structure, the presence of extractives, and its inferior mechanical properties complicate the production process, which have not yet been fully overcome at a commercial scale. This study proposes a paradigm shift, advocating for separate and specialized bark constituent processing in a wet state. Three bark-based raw materials-namely, outer bark particles, bark fiber clumps, and bark fibers-were investigated under varying wood content scenarios. PBs with a target density of 0.7 g/cm3 and a thickness of 16 mm were produced using mixtures of these bark-based materials and wood particles in different ratios bonded with a urea-formaldehyde adhesive. The results demonstrated that these bark constituents exhibit distinct properties that can be optimized through tailored processing techniques. Compared to bark fibers, outer bark particles displayed about 40% lower water absorption and thickness swelling. However, bark fibers improved the internal bond by about 50% due to their favorable morphology compared to outer bark. These findings highlight the potential of bark as a valuable resource for particleboard production and pave the way for its efficient utilization through specialized processing strategies.

Application of Date Palm Tree Branch-Based Activated Carbon for Aqueous Toxicity Reduction

The Kingdom of Saudi Arabia (KSA) has millions of date palm trees for commercial scale date-fruit production. The respective industry also generates agricultural waste including date palm tree branches. This rich bio-resource can be used for several beneficial applications. The present study therefore investigated the application of granular activated carbon (GAC) produced using date palm tree branches to successfully remove phenol, p-cresol, and copper from synthetic wastewater. The respective adsorption equilibrium results fitted well to the Langmuir-type adsorption isotherm. Furthermore, the pH-dependent adsorption results both for phenol and p-cresol appeared to follow an anionic-type adsorption behavior (i.e., decreasing adsorption with an increase in aqueous phase equilibrium pH). However, the pH-dependent adsorption finding for copper showed a cationic-type adsorption behavior. These adsorption trends were explained employing the pH-dependent speciation of the respective pollutants. In general, findings from the present work indicate that a high-specific-surface-area (SSABET) GAC material from the date palm tree branches can be successfully employed for aqueous phase pollution control.

The Vitextrifolia complex (Lamiaceae) in the Philippines.

The Vitextrifolia complex in the Philippines comprises economically important medicinal species, but its taxonomic status has become ambiguous due to numerous historical nomenclatural revisions. We assembled the complete chloroplast genomes of five species belonging to this species complex to provide additional evidence for their species delimitation. Based on a detailed analysis of specimens which combined molecular and morphological data, we propose reinstating V.elmeri Moldenke and delineating V.arvensis Gentallan, Sengun & M.B.Bartolome as a new endemic species belonging to this complex. The new species is a putative hybrid between V.bicolor Willd. and V.elmeri Moldenke. The specific epithet arvensis reflects its predominantly cultivated nature, both on a commercial scale and in home gardens, as a valued medicinal plant. We also provided a key to identify the five species belonging to the V.trifolia complex in the Philippines.

Waste-to-Energy technologies for municipal solid waste management: Bibliometric review, life cycle assessment, and energy potential case study

Inadequate municipal solid waste (MSW) management threatens public health and the environment. The waste-to-energy (WtE) route allows the production of electricity, heat, and other valuable chemical products. WtE consists of incineration, gasification, pyrolysis, and some emerging technologies. This study follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to conduct a bibliometric analysis of WtE technologies for MSW management research. Scopus-indexed articles were examined between 1971 and 2023. Four periods were defined and analyzed in terms of their most significant results. In addition, the most pertinent journals (Waste Management, Chemosphere, and Waste Management & Research) ranked first, second, and third, respectively. Moreover, the top contributor countries include one in America (the United States), three in Asia (China, Japan, and India), and five in Europe (Spain, Italy, Germany, Sweden, and the United Kingdom). Throughout a Life Cycle Assessment (LCA) case study, the environmental performance of gasification and incineration were compared as relevant technologies for treating MSW were contrasted. Gasification excelled in seven environmental parameters, suggesting that developing strategies for MSW gasification on a commercial scale should prioritize this aspect. Finally, the energy potential was assessed, which provided valuable information on the societal benefits of WtE technologies in various sectors, such as education, health, and electromobility. Under their respective contexts, stakeholders should enhance MSW sorting policies, pretreatment methods, and emerging valorization options. The growing global interest in WtE requires a social, environmental, and economic evaluation before implementation.

A comprehensive review of integrated management strategies for damping-off disease in chili.

Damping-off disease in chili (Capsicum annum L.) cultivation is a significant global issue, severely affecting seeds, seedlings, and young plants, regardless of the location of cultivation, whether in greenhouses or open fields. Despite chili being a widely popular vegetable used in various cuisines globally, farmers face challenges in meeting the growing demand due to the extensive damage caused by this disease, ranging from 20 to 85%. The shelf life and quality of mature pods are also severely affected. Damping-off disease is mainly caused by soil-borne fungus from the Pythium species, with additional contributions from Phytophthora, Fusarium, and Rhizoctonia species. These pathogens' adaptability to diverse environmental conditions and resistance to synthetic fungicides make controlling damping-off on a commercial scale challenging. However, integrated disease management has shown promising results as a remedial approach. In this review, we discuss the current state of chili diseases, the nature of the pathogens causing damping-off, the epidemiology of the disease, and various control mechanisms. In this review, we broadly discuss the current state of chili diseases, the nature of the pathogens causing damping-off, the epidemiology of the disease, and various control mechanisms. Furthermore, we highlight the importance and efficacy of integrated disease management techniques, along with future prospects in unexplored areas, such as host-pathogen interaction and sustainable disease control measures. The information in this review aims to assist chili growers in understanding the epidemiology and management of damping-off in chili cultivation.