Candida Albicans Colonies Research Articles

THE EFFECTIVENESS OF CINNAMON BARK EXTRACT PASTE (Cinnamomum burmanii) IN ELIMINATING Candida albicans IN THERMOPLASTIC NYLON

Background: One type of denture base that is widely used is thermoplastic nylon. Thermoplastic nylon not cleaned regularly will cause food residue to stick and give rise to fungi such as Candida albicans. Mechanical cleaning methods are more effective at removing microbes. However, regular toothpaste contains abrasive ingredients that can scratch dentures. Extracts from cinnamon bark can be used because they contain ingredients that can inhibit the growth of Candida albicans colonies. Purpose: To analyze the antifungal activity of using cinnamon bark (Cinnamomum burmanii) extract paste to eliminate Candida albicans on thermoplastic nylon. Methods: This research is purely experimental, using a pretest-posttest with a control group design. This study used 27 thermoplastic nylon plates measuring 65x10x3mm, which were divided into 3 groups, namely the cinnamon bark extract paste group with a concentration of 50%, Fresh 'n Brite paste as a positive control, distilled water as a negative control. Results: The results of the Kruskal-Wallis test show an effect based on treatment in each group. Conclusion: Fresh ‘n Brite denture paste as a positive control is more effective in eliminating Candida albicans on thermoplastic nylon than cinnamon bark extract paste with a concentration of 50% and distilled water as a negative control.

Mycotic flora characteristics of the endontic flora in patients affected by COVID-19

BACKGROUND: Owing to the fact that, presently, at a dental appointment, it is possible to observe a wide range of consequences arising after COVID-19, among which are observed lesions of the pulp and periodontium of the tooth, additional diagnostic methods should be introduced before choosing treatment tactics. In our previous study, we analyzed the contents of root canals of teeth in patients who had COVID-19 using bacterioscopy. Coccus flora was detected in all cases and elements of Candida yeast-like fungi were recorded in 89 cases (76.1%). Based on the obtained data, it was advantageous to perform microbiological examination of the endodontium in this group of patients to further study their physical and chemical properties and correct the treatment regimen in gangrenous form of chronic pulpitis and aggravated forms of periodontitis, which determined the relevance of the present study. AIM: To reveal the characteristics of the mycotic flora of the endodontium in patients who previously had COVID-19. MATERIALS AND METHODS: A bacteriologic study of root canal contents in patients who had previously undergone a new coronavirus infection at different times, diagnosed as “exacerbation of chronic periodontitis”, “chronic gangrenous pulpitis”, and “acute suppurative periodontitis”, was conducted to detect fungi of the genus Candida in the tooth root canal system, which was obtained during mechanical treatment with a sterile endodontic instrument. The patients (n=49) were divided into groups according to their final diagnosis: group 1, chronic gangrenous pulpitis (27 patients); group 2, acute purulent periodontitis (9 patients); and group 3, exacerbation of chronic periodontitis (13 patients). Then, the collected material was placed in a tube with Amies transport medium and sent to the laboratory. Seeding of the material was performed in sterile Petri dishes on Sabouraud agar by rubbing with a plastic spatula. Further, Petri dishes were placed in the thermostat for 24–48 hours of incubation at 37±10 °C. Then, the study was carried out according to the generally accepted scheme: the obtained cultures were identified to species by the character of growth on dense medium. RESULTS: The given bacteriological study of the patients’ endontic contents confirmed the data of bacterioscopy and concretized the previously obtained results. On final examination, black colonies of Candida albicans were observed in 95.92% of cases. No growth of Candida albicans colonies was observed in two cases (4.08%). CONCLUSION: The bacteriologic study performed after bacterioscopy of diagnosis confirmed the presence of fungi of the genus Candida in the root canals of teeth in patients who previously had COVID-19. The study showed that when treating similar patients, the high risk of infection of periodontal complex tissues by Candida fungi should be considered. It is advisable to supplement the standard protocols of endodontic treatment with physical exposure, for example, laser radiation, or additional medication with antifungal drugs, such as fluconazole.

Effect of Tea Tree Oil Addition to Denture Liners Against Candida albicans and Bond Strength to Acrylic Denture Bases.

To evaluate the effect of adding tea tree oil to denture liners on Candida albicans and bond strength to the acrylic denture base. Disc-shaped specimens were fabricated from silicone-based resilient liner (Tokuyama, Molloplast), acrylic-based hard liner (GC Reline), and acrylic-based soft liner (Visco-gel). Tea tree oil (TTO) was incorporated into the liners at varying concentrations (0% [control], 2%, 5%, 8%). C albicans were counted by viable colony count, and optical density (OD) was measured with a spectrophotometer. The tensile strength to heat polymerized acrylic denture base was measured in a universal testing machine. The compliance of the data to the distribution of normality was evaluated using the Shapiro Wilk test. Two-way ANOVA, Bonferroni correction, and paired sample t test were performed (α = .05). The addition of TTO into liners provided a significant decrease in the OD values (P < .001). The control groups of the liners presented the highest colony counts, whereas increasing TTO decreased the results (P < .01). According to tensile bond strength test, 8% TTO addition resulted in a significant decrease for Tokuyama (P < .01) and Molloplast liners (P < .05), while 2% TTO resulted in significance for GC Reline (P < .001). Denture liners containing increasing percentages of TTO presented lower amounts of C albicans colonies and decreased bond strength to the denture bases. When using TTO for its antifungal properties, the amount added should be carefully selected because the tensile bond strength may be affected.

In Vitro Effect of Low-level Laser Therapy on Candida albicans Colonies Isolated From Patients Undergoing Radiotherapy for Head and Neck Cancer.

Introduction: Studies on head and neck cancer (HNC) patients undergoing radiotherapy have revealed increased numbers of Candida spp., leading to oral candidiasis and oral mucositis. The effects of laser therapy on Candida spp. have been studied with varied results. This study aimed to investigate the effect of low-level laser therapy (LLLT) on C. albicans colonies isolated from HNC patients undergoing radiotherapy. Methods: This study included a treated group, wherein 11 C. albicans isolates were obtained from the saliva of HNC patients undergoing radiotherapy at a dose exceeding 30 Gy. The control groups consisted of an untreated negative control and a positive control treated with nystatin. The treatment groups were subjected to LLLT in continuous mode for 50 seconds at a wavelength of 976 nm, 0.1 W at a dose of 5 J/cm2 for Laser 1 (L1) group and 0.2 W at a dose of 10 J/cm2 for Laser 2 (L2) group. The tests were conducted using C. albicans inoculum equivalent to a 0.5-McFarland suspension on 96-well plates. All test group inoculums were then cultured on Sabouraud dextrose agar (SDA), and the colony count was assessed at 104 CFU/mL. Results: LLLT at 5 and 10 J/ cm2 reduced C. albicans colonies by 7.01% and 10.94%, respectively, but the reductions were not statistically significant (P>0.05). Nystatin eliminated all the colonies. Conclusion: LLLT shows potential for reducing C. albicans colonies in HNC patients undergoing radiotherapy. However, further studies with varied parameters and methods are necessary.

Potential of chitosan as anti Candida albicans in denture bases made from thermoplastic nylon

Background: Candida albicans is main cause of fungal infections in denture users. Prevention is needed by soaking dentures using a disinfectant. Chitosan is an alternative disinfectant emulsion because it is biodegradable, biocompatible, and does not cause discoloration. This research objective is to determine the effect of chitosan emulsion on the number of C.albicans on base plate thermoplastic nylon.&#x0D; Method: This is a true experimental study using post-test only design by using 25 base plates divided into 5 groups namely treatment I, treatment II, treatment III, positive and negative control. Plate was incubated in C.albicans suspension for 24 hours in McFarland and 10-3 series dilution. The plates was rinsed with aquadest, then soaked with 0.3% chitosan emulsion, 0.8% chitosan emulsion, 2% chitosan emulsion, 0.2% chlorhexidine gluconate, and aquadest for 15 minutes. The attached C.albicans colony in the plate was released to the SDB by using centrifuge device, then proliferated on the SDA and incubated (24 hours). The results of the C.albicans colony were counted visually by using a colony counter. The number of Candida albicans colonies data were analyzed using One-Way ANOVA and then Post Hoc Tukey HSD test.&#x0D; Result: The results showed that there were significant mean differences between each group (p

Correlation between use of different type protective facemasks and the oral ecosystem

BackgroundSpread worldwide through droplets, the Virus Sars-Cov-19 has caused a global health emergency alarm. In order to limit its spread, the use of masks has become part of the daily life of the entire population, however, little is known about its constant use and the changes generated in the oral cavity. This work aims to investigate correlations between the continuous use of masks covering the nose and mouth for 3 h and changes in the ecological factors of the oral cavity.Methods34 volunteers were divided into 2 groups: wear only the filtering facepiece code 2 (FFP2) mask (Group A) and wear the FFP2 mask covered by a surgical mask (Group B). Measurement of Volatile Organic Compounds (VOCs), saliva rehydration and consistency test, collection of basal saliva and saliva stimulated with paraffin gum and mucosal swab were collected and analyzed at two times: before using the mask(s) (T0) and 3 h after continuous use of the mask(s) (T1).ResultsThe results indicated a significant difference between the groups, in which the basal saliva volume and pH and the peaks of VOCs increased for group B between T0 and T1. The rehydration time decreased and the volume and pH of the stimulated saliva increased, but with no significant difference between the groups. Furthermore, group B showed a significant decrease in Candida albicans Colony Forming Units (CFUs) and Total Bacterial Count (TBC) between T0 and T1.ConclusionIt is concluded that the prolonged use of the FFP2 mask covered by a surgical mask can generate oral alterations in the user.

EVALUATING CHANGES IN SALIVARY FLOW RATE, PH &amp; MICROBIAL COUNT DURING FIXED ORTHODONTIC TREATMENT - AN IN VIVO STUDY

Aim of the study: Aim of this study is to assess the changes in salivary flow rate, salivary pH, plaque index, Streptococcus mutans, Lactobacillus, and Candida albicans in patients undergoing fixed orthodontic treatment, before and after placement of fixed orthodontic appliances. Materials and Method: In present study salivary samples from 60 patients (30 Males and 30 Females) with age group of 14-25 years would be undergoing fixed orthodontic treatment at the department of orthodontics. Salivary flow rate, pH, plaque index and microbial colony count were measured. Streptococcus mutans, Lactobacillus and Candida albicans colony counts were measured using mitis salivarius agar, rogosa agar and sabouraud dextrose agar respectively. Saliva collected for 5 mins and measured in ml/min. salivary pH measured with digital pH meter. Salivary samples obtained from the same patients at intervals of 8 weeks and 16 weeks after commencement of orthodontic treatment and evaluated for the same. Result: This study showed fixed orthodontic appliances increases salivary flow rate (p=0.0001), decreases salivary pH (p=0.03), increases plaque index (p=0.0001), also increases Streptococcus mutans, Lactobacillus and Candida albicans colony count (p=0.0003, p=0.0001and p=0.019 respectively). Independent t test reported salivary flow rate significantly increases more in male patients at 16 weeks interval (p=0.03). Conclusion: The results of this study confirmed that after placement of fixed orthodontic appliances plaque retention increases, which sequentially decrease the salivary pH and decreased salivary pH promote the growth of microbiota.

Investigation of active laser delivery and antimycotic activity of zinc-containing nanomaterials and photodynamic drugs in the treatment of onychomicosis

One of the most common nail pathologies is onychomycosis, a fungal nail infection. The low efficiency of existing methods for the treatment of onychomycosis stimulates the search for new drugs and technologies for their delivery under the nail plate. The aim of the investigation was to study the possibility of using Er:YLF laser radiation for sequential laser microporation of the human nail plate and active laser delivery of modern photodynamic preparations and zinc-containing nanomaterials under the nail plate, as well as to determine their antimycotic activity against the Candida albicans fungus under photodynamic exposure with a wavelength of 656±10 nm. A 0.001 % aqueous solution of methylene blue, a chlorine-containing Revixan gel, as well as zinc-containing nanomaterials, which are aqueous solutions of polyvinylpyrrolidone gel with different concentrations of Zn(NO3)2 nanoparticles and acidity, were studied. It was established that the smallest number of laser pulses required for in vitro microporation of the nail plate and active laser delivery under the nail plate is required when using a 0.001 % aqueous solution of methylene blue, and the largest is required when using Revixan gel. For zinc-containing nanomaterials, an increase in the concentration of Zn(NO3)2 nanoparticles from 14 to 40 % leads to an increase in the number of laser pulses required for active drug delivery under the nail plate by 1.25 times, while a change in the acidity of the pH gel from 7.0 to 6.7 does not has a significant impact on it. It was found that all the studied drugs in combination with photodynamic action at a wavelength of 656±10 nm have an antimycotic effect on the Candida albicans fungus culture. The minimum growth rate of Candida albicans colonies was observed when using the Revixan gel, for which the antimycotic activity reached 73.8 %. The obtained results can be applied in the development of laser systems and technologies for the treatment of fungal diseases, including for photodynamic therapy of onychomycosis.

Effect of water treatment with low-temperature and low-pressure glow plasma of low frequency on the growth of selected microorganisms

ABSTRACT Tap water treated in air with low-temperature and low-pressure glow plasma of low frequency was tested for its either stimulation or inhibition of the growth of the selected microorganisms commonly colonizing human organism. The growth of chosen microorganisms was monitored by estimation of optical density of their colonies. The fairly linear growth against time of all microorganisms under study accelerated after 12 h from the beginning of the experiment. Colonies of E. coli and S. cerevisiae breed in the plasma treated water had an approximately 20% stimulation of the growth which was observed between 12 and 24 h. Neither stimulation nor inhibition of the growth could be noted for colonies of Aspergillus niger, Candida albicans, Yarrowia lipolytica, and Enterococcus faecalis, in whole period of observation. The plasma-treated water had no effect upon the growth of Mycobacteria. Independently of the water tested, M. tuberculosis started proliferating on the 14th day of the experiment, M. intercellulare and M. kansai after 9 days, and the growth of M. fortuitos could be observed after 3 days.

Incidence and prevalence of oral candidal colonization in patients with cleft lip and palate.

This study aims to assess and compare the incidence and rate of oral candidal colonization in patients with cleft lip and palate and collate it with healthy individuals. The objectives of this study were to evaluate the factors that may affect the incidence, rate, and polycolonity of colonization of oral Candida species in patients with cleft lip and palate (CLP) as compared to healthy individuals. The current prospective study involved the collection and assessment of oral samples from 50 CLP patients and compared it with oral samples from 50 healthy individuals. Colonies of Candida albicans and other Candida species were identified by using germ tube test and differential chromogenic media. Assessment of the distribution trend of various Candida species and their variation based on age, gender, type of cleft, number of surgeries, and oral hygiene status were recorded. A highly significant (P value < 0.001) statistical difference was seen in the oral carriage rate of Candida species between the two groups with values more in the study group (60.75 ± 71.43) than in controls (1.62 ± 2.22). Individually comparing the different Candida species, significant difference in presence of C albicans (P value = 0.004), C parapsilosis (P value = 0.015), and C dubliensis (P value = 0.027) was seen as compared to other species. In the context of the type of cleft and the presence of Candida species Candida albicans was the most prevalent in patients with the cleft alveolus (60.0% n = 15), unilateral CLP (66.7% n = 8), and bilateral CLP (100.0% n = 3) respectively. Nonsignificant variation in prevalence was seen in the various fungal species based on an individual's gender and oral hygiene and the number of surgeries status was observed. The anatomical alteration in the oral cavity of patients with CLP contributes to the high incidence and prevalence of Candida species.

Antimicrobial effect of silver nanoparticles on polypropylene and acrylic resin denture bases.

The investigation of the antimicrobial action of Ag solutions in different surfaces has been performed, however, there is little data on the direct addition of those particles to the polypropylene denture base. The aim of this study was to evaluate the antimicrobial effect of silver nanoparticles in Candida albicans and Streptococcus mutans colonization in acrylic resin and polypropylene denture bases. Twenty test bodies (10×13×3 mm) were fabricated with (heat-polymerized) poly methyl methacrylate acrylic resin (group 1), (heat-polymerized) poly methyl methacrylate acrylic resin with silver nanoparticles (group 2), polypropylene (group 3) and polypropylene with silver nanoparticles (group 4). Candida albicans and Streptococcus mutans colonization were performed. A colony count was performed in Colony Forming Unit (CFU/ml) and registered to calculate the arithmetical mean of the duplicate values of each sample. Data was analyzed by Lilliefors test and since samples did not present a normal distribution, Kruskal-Wallis for variance analysis with the value of significance set at p<0.05. For Candida albicans colonies, when comparing nano-Ag in polypropylene, there were statistical difference between groups 3 and 4. For the same type of growth in heat-polymerized PMMA resin with and without nano-Ag, no statistical differences were found. The presence of nanoparticles did not affect Streptococcus mutans growth in the heat-polymerized PMMA resin. Value differences of S. mutans were not significant for comparisons between groups 1-2 and 3-4. Silver nanoparticles presented antifungal activity against C. albicans in polypropylene surfaces, but not in the heat-polymerized PMMA resin. Silver nanoparticles did not present antibacterial activity against S. mutans in any of the analyzed surfaces. Key words:Removable Partial Denture, Candida, Streptococcus mutans.

Effectiveness of Kemangi (Ocimum basilicum) Leaf Methanol Extract against Candida albicans Colonies

Candida species, for women are the foremost common cause of parasitic contaminations. Candida species affect contamination in 75% of women and at slightest 6-9% of women involvement repetitive vulvovaginal candidiasis. Candida albicans (CA) accounts for 85-95% of yeast strains separated from the vagina. The treatment which has been administered for candida infections is antifungal drugs such as clotrimazole and fluconazole. When applied topically, synthetic antifungal drugs cause allergic reactions, resistance, and a burning sensation. It is necessary to conduct research on plant-based herbal medicine as an alternative treatment. Kemangi, also recognized as Ocimum Basilicum (OB), is a plant native to Indonesia which has medicinal properties. The objective of this study is to examine how effective OB methanol extract is against CA colonies. The study was performed at Brawijaya University's Microbiology Laboratory in Malang. The experimental laboratories with Posttest Only Control Group Design were employed in this study, with four repetitions of OB concentrations of 15%, 20%, 25%, 30%, and 35% against CA colonies. One-way ANOVA was utilized as the hypothesis test, with a significance level of 0.05. The results demonstrated that OB extract with a concentration of 15% was able to inhibit the growth of CA colonies. In the OB extract with a concentration of 35%, no CA colony growth was revealed. One-way ANOVA test obtained p 0.000 &lt;0.05. Conclusion OB owns adequacy in restraining the development of CA organism with negligible murdering rate at a concentration of 35%. Research required to be performed to identify the antifungal potential of OB extract in vivo.

Effect of method and concentration of titanium dioxide addition on anti-biofilm ability in extraoral maxillofacial prosthetic fungus

Background: One of the efforts to prevent adhesion and biofilm colonization on the material surface is to improve its properties by using Titanium dioxide (TiO₂) nanoparticles. TiO₂ nanoparticles have antimicrobial properties, especially against the fungus Candida albicans as it has photocatalytic properties that can inhibit the growth of the fungal colonies. The present study aimed to determine the effect of applying TiO₂ to polyurethane plates on the growth of Candida albicans fungal biofilms.Methods: This study applied a laboratory experimental design. The subjects were divided into two groups which included the treatment and control groups. In the treatment group, there were two treatments consisting of the addition of TiO₂ filler (1%, 2%, 3%, and 4%) and the material surface coating group with TiO₂ (1%, 2%, 3%, and 4%). Candida albicans was cultured and grown to form biofilms on polyurethane plates in each group.Results: The results of the statistical analysis obtained through Welch's One Way Anova showed that there was a significant difference in the number of Candida albicans colonies between the treatment and control groups (P&lt;0.05). Treatment with 4% TiO₂ surface coating showed the lowest number of Candida albicans colonies.Conclusions: Coating the surface of the material with TiO₂ on a polyurethane plate was able to inhibit the formation of Candida albicans biofilms.

Utilization and potential of natural materials in prostodontics: literature review

Antibiotics and antifungals are needed in the patient's dentures because acrylic resin denture base can be a breeding ground for Streptococcus mutans and Candida albicans colonies. Indonesia has biodiversity consisting of thousands of plant species so that it becomes an opportunity for the development ofsecondary metabolites for the healthcare industry. Some natural materials can be used as antibiotics, anti-inflammatory, and antifungal. Antibiotics and antifungalsare needed in the patient's dentures be-cause acrylic resin denture base can be a breeding ground for S.mutans and C.albicans colonies. This literature review explains the utilization and potential of natural materials in prosthodontics. So, it is concluded that various active compounds such as fla-vonoids, alkaloids,saponins, steroids, tannins, essential oils, kabivetol, estrgiol, eugenol metileugenol, carvakrol, phenol, trape-zoid, ascorbic acid and others found in natural materials have antifungal, antibacterial, and anti-inflammatory properties.

Efektivitas pertumbuhan Candida albicans pada media alternatif air rebusan kacang kedelai (Glycine max (l) Merr)

Background: Candida albicans are one of the pathogenic fungi in humans whose growth requires carbohydrates and proteins to grow and develop. Soybeans have a reasonably good nutritional content and potential as an alternative medium for the growth of Candida albicans fungi, and soybeans are very easy to find in the community, so they can be used as an alternative medium to replace SGA (Sabouraud Glucose Agar). Soybeans (Glycine max (L.) Merr) have nutritional content consisting of oils, carbohydrates, and minerals that allow them to source nutrients and food for fungi. Furthermore, one of them that can be utilized from soybeans is cooking water.Objectives: The study aims to determine whether soybean (Glycine max (L.) Merr) cooking water media can be an effective alternative for Candida albicans culture.Methods: An analytical study using an experimental design was conducted in the Microbiology laboratory at the Department of Medical Laboratory Technology, Health Polytechnic of Aceh, Ministry of Health, in September 2022. The sample used in this study was soybean cooking water as an alternative medium for Candida albicans. Data collection is based on the culture (breeding) of Candida albicans on alternative media with soybean decoction water as raw material. For the identification of Candida albicans species, confirmation tests were carried out using the germ tube test method. Then the growth assessment of positive (+) colonies of Candida albicans on alternative media of soybean cooking water was incubated for 72 hours (± three days) at 37°C. Data analysis was only done descriptivelyResults: There is the growth of Candida albicans fungal colonies on alternative media from soybean cooking water with macroscopic characteristics of yellowish white color, yeast smell, smooth, smooth surface, flat edges, small colonies, and many colonies. On microscopic observation, Candida albicans fungus was found with microscopic characteristics of round, oval, small, thin-walled, yeast-like cells and pseudohyphae.Conclusion: Soybean (Glycine max (L) Merr) cooking water media is effectively used as an alternative media for the growth of Candida albicans.

In Vitro Effect of a High-Intensity Laser on Candida albicans Colony Count.

Introduction: This study assessed the effect of a high-intensity laser on Candida albicans colony count. Methods: This in vitro, experimental study was conducted on standard-strain C. albicans (ATCC18804). Seventy-two samples with two different concentrations of C. albicans (104 cells/mL and 106 cells/mL) were randomly assigned to three groups of control, laser and nystatin. Each group included 12 samples from each concentration. In the nystatin group, 10 cc of nystatin suspension was added to the samples and mixed for 30-60 seconds. In the laser group, the Ga-Al-Ar diode laser with a 940-nm wavelength and 2-W power was irradiated to the samples with 0.4 mm tip diameter in non-contact mode (1-mm distance) at a speed of 1 mm/s. The suspensions in the three groups were cultured on the Sabouraud dextrose agar culture medium and incubated at 37°C for 24 hours. The number of C. albicans colonies was then counted and reported. The three groups were compared by ANOVA and Tukey's test (alpha=0.05). Results: In both concentrations, the mean colony count in the nystatin group was significantly lower than that in the control (P<0.05) and laser (P<0.05) groups. The mean colony count in the laser group was significantly lower than that in the control group (P<0.05). Conclusion: The high-intensity laser with a 940-nm wavelength and 2-W power in non-contact mode can significantly decrease the C. albicans colony count in vitro, but its effect is lower than that of nystatin.

Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract

The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.

Unusual cause of bronchiolitis obliterans in an immunosuppressed patient

Abstract Bronchiolitis obliterans organizing pneumonia (BOOP) is a diffuse interstitial lung disease, currently classified as cryptogenic organizing pneumonia (COP) when idiopathic, respectively organizing pneumonia (OP) in case of a specified etiology. We present the case of a 48-year-old patient, former smoker (20PY), diabetic, who accused moderate dyspnea, persistent febrile syndrome. The chest radiography showed multiple areas of condensation distributed bilaterally diffusely and the laboratory data revealed an important inflammatory syndrome. More than 100 colonies of Candida albicans were isolated from the sputum, therefore antifungal therapy was initiated. The computer tomography scan (CT scan) highlighted multiple micronodular and nodular lesions, some with tendency of excavation and hydroaeric level present and a polyseptate lesion in the right lower lobe; no proliferative elements were found during bronchoscopy and the bronchoalveolar lavage was non-specific; To obtain a final diagnosis, a CT-guided transthoracic needle biopsy was performed and the histological examination was compatible with organized pneumonia.

The Effect of Dentures’ Overnight Soaking in Distilled Water: A Mycological Pilot Study

Introduction: In order to keep their dentures clean and properly stored, patients generally soak them in water at night. Candida albicans is a commensal yeast fungus that colonizes dentures, and in some conditions, it becomes an opportunistic pathogen and causes fungal infections known as candidiasis.&#x0D; Objective: This pilot study aimed to evaluate the effect of distilled water on Candida albicans colonizing dentures.&#x0D; Materials and Methods: Twenty patients (9 men, 11 women; age range 40-75 years) with complete maxillary dentures infected by Candida albicans were included in this study. The dentures of these patients were soaked in distilled water for 4 days (8 hours at night). Swab samples from the dentures were collected before and after distilled water use and examined mycologically.&#x0D; Results: The Candida albicans colony counts increased after soaking the dentures in distilled water for 8 hours for 4 days.&#x0D; Conclusion: Patients should be dissuaded from soaking their dentures overnight in distilled water as the result is a significant increase in fungal colonization.

Growth of Candida albicans colony on heat cured acrylic plat in inclusion of lime fruit extract (Citrus aurantifolia) 40% as desinfectant

Heat cured acrylic (HCA) resin has a rough, hard and porous surface. This causes food scraps to stick frequently and makes it easier for Candida albicans to grow. Lime peel extract (citrus aurantifolia) is a natural ingredient that is antifungal. The pur-pose of this study was to determine the growth of C.albicanscolonies on heat cured acrylic dentures in 40% immersion of lime peel extract. The HCA resin plate samples were 40 samples, namely the 40% lime peel extractconcentration group, the negative control group (akuades) and the positive control group which were soaked for 2 hours and 6 hours. The design of this study was a post-test only control group with data analysis using the one-way Anova test. The results showed that each group was normally distributed and the variance data was heterogeneous (p&gt;0.05). It is concluded that 40% lime peel extract can inhibit the growth of C.albicans colonies on HCA resin plates.