An imbalance between flavonoids depletion and lipid metabolism may be a key mechanism of chilling injury in cold-stored cucumbers.

Normothermic machine perfusion (NMP) enables metabolic restoration and viability testing of liver grafts, but current viability criteria incompletely predict post-transplant outcomes. The molecular basis of graft resilience or biliary vulnerability remains unclear. This study aimed to characterise tissue-level proteomic trajectories during NMP and early reperfusion to identify molecular signatures associated with biliary complications after liver transplantation (LT). This prospective, single-centre study was conducted at Rennes University Hospital. Twenty donation-after-brain-death (DBD) livers underwent NMP; sixteen transplanted grafts with complete sequential biopsies and ≥ 6 months of follow-up were analysed. Biopsies were collected after cold storage (B1), at the end of NMP (B2), and 1 h after graft reperfusion (B3). Proteins were quantified by high-resolution LC-MS/MS and analysed with Proteome Discoverer 3.1/Chimerys. Pathway enrichment used Ingenuity Pathway Analysis to compare grafts with and without biliary complications. Principal component analysis revealed distinct proteomic profiles between grafts with and without complications at all biopsy time points. During NMP (B2/B1), uncomplicated grafts showed glycolytic activation with attenuation of oxidative phosphorylation, whereas complicated grafts showed blunted glycolysis and mild OXPHOS upregulation. At reperfusion (B3/B2), complicated grafts displayed induction of translational and endoplasmic-reticulum-stress pathways, while resilient grafts maintained proteasome-related protein turnover and enrichment of a hypoxia-response signature driven by ELOC and proteasome subunits. Sequential tissue proteomics during NMP reveals divergent metabolic and proteostatic adaptations linked to biliary outcomes. Glycolytic activation with preserved protein turnover characterises resilient grafts, whereas translational and ER-stress programmes predominate in complicated ones. These insights may refine viability assessment beyond biochemical criteria.

R2R3-MYB transcription factors in regulating postharvest quality of fruit and vegetables during cold storage: Mechanisms and prospects

Preservation of pediatric donor hearts via subnormothermic machine perfusion: Comparison to cold storage.

Sustainable valorization of unutilized marigold flowers for eco-friendly herbal gulal production and its storability effects on quality attributes.

Peach fruit are highly perishable and prone to chilling injury (CI) during cold storage, limiting postharvest storage life. We evaluated the efficacy of low-pressure (LP) storage alone and in combination with 1-methylcyclopropene (1-MCP) to delay maturation, mitigate CI, and thus extend the storage life of ‘Early Rich’ peaches. Fruit were stored under atmospheric pressure (AP) or LP storage conditions without or with 1-MCP and were evaluated after 21 and 35 d of cold storage, followed by simulated shelf life conditions at 20 °C. LP storage reduced weight loss and CI incidence significantly compared with AP controls. The combination of LP and 1-MCP resulted in the greatest firmness retention during shelf life, although the resulting firmness was greater than typical ripe eating values (30–35 N), suggesting a possible oversuppression of ripening by the 1-MCP dosage. Alternatively, this may suggest that the storage durations could be much longer before softening becomes the limiting postharvest factor. Internal quality parameters (soluble solids content and titratable acidity) were largely preserved under LP storage, despite greater respiration rates than expected. These results indicate that LP storage, particularly when combined with optimized 1-MCP dosing, can extend the storage life of ‘Early Rich’ peaches by ∼1 to 2 weeks while maintaining marketable quality.

Inside the kiwifruit microcosm: Tissue-specific microbiome-metabolome shifts driven by cold storage and 1-MCP

Optimized oligochitosan treatment for preserving postharvest quality of Sepia pharaonis during cold storage

Combined analysis of microbial diversity, water migration, and volatile spoilage profile changes in modified atmosphere packaged crucian carp (Carassius auratus) fillets during cold storage

Dual cross-linked alginate-tara gum hydrogel embedded with red cabbage anthocyanins as a smart indicator for non-destructive fish freshness monitoring.

Different strategies to obtain laccase-based time-temperature indicator for monitoring of cold chain storage and management

Exposure to medication during pregnancy can compromise placental function, impacting maternal and fetal health. This study aims to evaluate the viability of human precision-cut placental slices (hPCPSs) as a potential in vitro model, offering a new platform for experimental investigations in placenta. Decidual and villous hPCPSs of healthy, full-term placentas were prepared with a Krumdieck slicer and were cultured and analyzed at 0 h, 24 h, 48 h, and 72 h. Slice viability was assessed using ATP/protein content, lactate dehydrogenase (LDH) leakage and PrestoBlue assay. Progesterone, estradiol, estrone and human chorionic gonadotropin secretion were measured in culture supernatants. Morphological changes were microscopically evaluated via Hematoxylin and Eosin (H&E) staining. To evaluate the impact of delayed processing, slices from freshly processed placentas and stored at 4 °C for 24 h were also compared. At 72 h, ATP decreased only in stored villous hPCPSs (P < 0.01), whereas PrestoBlue indicated reduced viability in all groups except fresh villous (all P < 0.05). LDH leakage peaked at 24 h across decidual and villous slices, irrespective of storage (P < 0.05). Hormone levels peaked at 24 h and subsequently decreased, with significance mainly in progesterone and estrone (P < 0.05). H&E showed progressive karyorrhexis and syncytiotrophoblast detachment across all groups. Our studies established a reproducible protocol for generating hPCPSs, enabling separate evaluation of decidua and villi. Furthermore, our results underscore the importance of viability monitoring and suggest that hPCPSs maintain viability up to 48 h, even after short-term cold storage.

A real-time modeling approach of transient thermal behavior for phase change cold storage heat exchanger

Feasibility of Lung Hypothermic Perfusion Without Ventilation: An Experimental Study.

Suitable donor shortage for lung transplantation and limiting criteria of circulatory death donors calls for the expansion of donor criteria. A critical parameter for lung quality is oxygenation. We investigated the influence of ventilation on lung injury resulting from circulatory death utilizing a lung perfusion protocol. Circulatory dead male Wistar rats were ventilated or not for 30 min during warm ischemia. Lungs were either placed in cold storage for 2 h; or perfused in situ for 2 h with Perfadex in an open circuit (37°C). Perfusate was collected at 15 and 120 min of perfusion. Lung tissue was evaluated for inflammation and apoptosis. Lung fragments were placed in culture (24 h), and medium was used to measure cytokine concentration. Cytokines were found elevated in the perfusate at the end of perfusion: tumor necrosis factor-α (P = 0.0312) and (interleukin (IL)-6 P = 0.0049). These cytokines in lung homogenates were higher in the perfused groups compared to their respective controls: tumor necrosis factor-α (P = 0.0036), IL 1-β (P = 0.0011), and IL-6 (P = 0.0595). Ventilation increased lung BAX (Bcl-2 associated X protein) expression in the control groups (P = 0.0248) and indicated a higher number of caspase-3-stained cells (P = 0.0185) when compared to the nonventilated groups. A decrease in hemorrhage was observed after perfusion in both groups (P < 0.0001). Ventilation during warm ischemia seems to point to an increased apoptotic profile of donor lungs. Thus, early ventilatory strategies might not be necessary for graft maintenance, and the data presented supports the potential inclusion of uncontrolled circulatory dead donor lungs to expand the donor pool for transplantation.

RF36. Celsior Cold Storage Reduces Ischemic Injury in Human Donor Hearts from Circulatory Death by Alleviating Myocardial Endoplasmic Reticular Stress and Inflammation

Normothermic machine perfusion (NMP) and normothermic regional perfusion (NRP) have expanded donation after circulatory death (DCD) liver transplantation (LT). However, cold ischemia time (CIT) potentially restricts access to DCD LT for recipients living far from transplant centers. The impact of perfusion technology on DCD LT access for geographically distant patients remains underexplored. All adult DCD LT at a transplant center from January 2017 to December 2024 were reviewed. Recipients were categorized by perfusion method:static cold storage (SCS) only, NRP, or NMP. Real-world travel distances and times to transplant center were calculated using Google Maps application programming interface. Social determinants of health, including Distressed Community Index (DCI) scores, were assessed. Among 208 DCD LT recipients (n = 80 SCS; n = 106 NRP; n = 22 NMP), median travel distances were 63 (17-122), 61 (23-92), and 156 (78-386) miles for SCS, NRP, and NMP, respectively (NMP versus SCS and NRP, p = 0.043 and 0.010). Median travel times were 1.23 (0.38-2.40), 1.20 (0.50-1.80), and 3.15 (1.58-7.73) h for SCS, NRP, and NMP, respectively (NMP versus .SCS and NRP, P = 0.025 and 0.007). Although DCI distribution did not differ by procurement type, New Mexico (NM) recipients had significantly higher DCI scores (67.7, at risk) than Colorado (CO) recipients (26.7, comfortable) ( P < 0.001). Notably, 21% of NM recipients received NMP LT compared with 7% of CO residents. An early center experience with DCD LT demonstrates that perfusion technologies facilitated transplantation for geographically vulnerable recipients. NMP recipients on average lived over 2 times farther away and traveled twice as long compared to SCS and NRP recipients. NMP allowed us to mitigate historical barriers of prolonged CIT, especially for recipients from NM, who live further and in highly distressed communities.

Preparation and optimization of graphene-doped TiO2 shell microencapsulated phase change materials for high-stability cold storage

PP2A inhibition alleviates DCD liver damage during prolonged cold ischemia by interfering Drp1 translocation and ER stress.

Light spectrum ratios during cultivation affect antioxidant capacity and chilling tolerance of cold storage holy basils