Co-digestion Of Food Waste Research Articles

Effects of mixing time on methane production from anaerobic co-digestion of food waste and chicken manure: Experimental studies and CFD analysis

The relationship between mixing time and methane production was investigated by anaerobically co-digesting food waste (FW) and chicken manure (CM) at four different organic loading rates. The mixing pattern and turbulence intensity obtained from CFD were adopted to evaluate the mixing performance in digesters with different viscosities. The simulated mixing time from CFD was selected as a reference for the first time to analyze the methane yield. The results showed that if the digester was well mixed under intermittent mixing mode with relatively short mixing time, then extending mixing time or changing intermittent mixing to continuous mixing would have no substantial effects on methane production. By contrast, continuously mixed digesters performed better when the intermittent mixing modes cannot make the digester get to a high degree of uniformity. Hence, the simulated mixing time from CFD can be used as a reference to determine the experimental mixing time in different cases.

Anaerobic co-digestion of food waste with pretreated yard waste: A comparative study of methane production, kinetic modeling and energy balance

Abstract Solid state anaerobic digestion of food waste (FW) with yard waste (YW) appears to be an efficient technique for bioenergy production. However, the reluctant nature of YW and nutrient imbalance due to high C/N ratio are the two major obstacles for maximum methane production. In this study to improve the methane production potential, YW was thermally pretreated to reduce its recalcitrance nature and then the pretreated YW was co-digested with FW to balance nutrient for anaerobic co-digestion. Moreover, optimization of F/M (Food/Microorganism) ratios (1.0, 1.5, 2.0 and 2.5) for solid state anaerobic co-digestion was also considered. The highest methane production of 431 mL/gVSadded was observed for co-digestion of FW with microwave pretreated YW at the F/M ratio of 1.5. Energy balance of the pretreatment technique was conducted and the highest net energy gain 3.98 kJ/gVS (= output energy of 14.04 kJ/gVS - input energy of 10.05 kJ/g VS) obtained at the F/M ratio of 1.0.

Simultaneous synergistic effects of addition of agro-based adsorbent on anaerobic co-digestion of food waste and sewage sludge

The aim of this study was to evaluate the performance of anaerobic co-digestion of food waste (FW) and sewage sludge (SS) at high organic load with the aid of an inorganic adsorbent supplementary. From BMP tests of seven mesophilic reactors, it was found that the stability of the system was hindered at higher organic load due to low alkalinity capacity of sludge (1600 mg/L) and excessive accumulation of inhibitory substances such as free ammonia and VFA. Thus, the low-cost adsorbent was applied to enhance the digestion process stability at high organic load (12.8 g VS/L). The results showed that addition of 1 g of sorghum-based activated carbon (SAC) (4 g TS/L) stabilized the system, reduced ammonia and T-VFA concentration from 267 to 39 mg/L, and 1300 to under 600 mg/L, respectively. Consequently, the methane yield increased from 201 to 272 mL/g VS (35%), solid retention time (SRT) reduced by 34%, and T-COD removal achieved by 79.38%. However, with a further increase of SAC the biogas generation decreased due to excessive adsorption of VFAs.

Optimizing pre-treatment conditions for anaerobic co-digestion of food waste and sewage sludge

In this study, the optimum conditions of thermal-alkali pre-treatment and the performance of ammonia stripping were investigated for improving solubilization efficiency and methane yield in the anaerobic co-digestion of food waste (FW) and sewage sludge (SS). The reaction temperature, NaOH concentration and reaction time for the thermal-alkali pre-treatment were investigated to determine optimum pre-treatment conditions. Solubilization rate, volatile suspended solids (VSS) reduction rate and total volatile fatty acid (VFAs) yields were improved with increasing reaction temperature, NaOH concentration and reaction time. In addition, by applying the optimum pre-treatment conditions (140 °C, 60 meq/L and 60 min), the experimental methane yield of thermal-alkali pre-treatment of a mixture of FW and SS was 483.0 ± 15.7 mL CH4/g VSadded, which was about 84% higher than that of the untreated one. However, after thermal-alkali pre-treatment, the NH4+ concentration of the thermal-alkali pre-treatment liquid showed a concentration that could inhibit anaerobic digestion, so ammonia stripping was performed to remove NH4+. As a result, the experimental methane yield was increased by about 7% compared to when ammonia stripping was not performed.

Kinetics Modelling of Batch Anaerobic Co-digestion of Domestic Primary Sewage Sludge and Food Waste in a Stirred Reactor

Anaerobic digestion was conventionally applied for treating sewage sludge. However, the accumulation of solid waste particularly food waste has reach the critical levels worldwide. In practice, the food waste was dumped into the landfill for ultimate disposal. However, the greenhouse gases produced in the landfill makes this is no longer a preferable option. Anaerobic digestion was seen as an alternative for managing the food waste in a sustainable way. Methane, a renewable energy is potentially in replacing fossil fuel. However, the methane yield from the digestion of food waste inefficient. Therefore, a study of the co-digestion of sewage sludge and food waste was conducted to investigate the improvement of the methane yield. This study was conducted by using a mixture of domestic primary sewage sludge and food waste as a co-substrate for the anaerobic digester. The kinetics modified Gompertz modelling was applied to describe the anaerobic digestion process. A series of batch biochemical methane potential (BMP) assay was prepared using Automatic Methane Potential Test System (AMPTS II) to investigate the anaerobic digestibility of the mixture of domestic primary sewage sludge and food waste. The BMP assay showed that the co-digestion improved the ultimate methane yield by 32.6% higher than domestic primary sewage sludge alone, indicated that the co-substrate characteristics influencing the methane yield. Besides that, the greater VS/TS ratio of the substrate also resulted in the greater methane yield. The kinetics parameter from the modelling analysis were slightly lower as compared to the laboratory data.

Anaerobic co-digestion of food waste and waste activated sludge: ADM1 modelling and microbial analysis to gain insights into the two substrates’ synergistic effects

The reasons for the acidification problem affecting Food Waste (FW) anaerobic digestion were explored, combining the outcomes of microbiological data (FISH and CARD-FISH) and process modelling, based on the Anaerobic Digestion Model n°1 (ADM1). Long term semi continuous experiments were carried out, both with sole FW and with Waste Activated Sludge (WAS) as a co-substrate, at varying operational conditions (0.8–2.2 g VS L−1 d−1) and FW / WAS ratios.Acidification was observed along FW mono-digestion, making it necessary to buffer the digesters; ADM1 modelling and experimental results suggested that this phenomenon was due to the methanogenic activity decline, most likely related to a deficiency in trace elements. WAS addition, even at proportions as low as 10% of the organic load, settled the acidification issue; this ability was related to the promotion of the methanogenic activity and the consequent enhancement of acetate consumption, rather than to WAS buffering capacity.The ability of the ADM1 to model processes affected by low microbial activity, such as FW mono-digestion, was also assessed. It was observed that the ADM1 was only adequate for digestions with a high activity level for both bacteria and methanogens (FISH/CARD-FISH ratio preferably >0.8) and, under these conditions, the model was able to correctly predict the relative abundance of both microbial populations, extrapolated from FISH analysis.

Effects of Two-Stage Operation on Stability and Efficiency in Co-Digestion of Food Waste and Waste Activated Sludge

The two-stage anaerobic digestion (AD) technology attracts increasing attention due to its ability to collect both hydrogen and methane. A two-stage AD system feeding with food waste and waste activated sludge was investigated in order to achieve higher energy yield and organics removal. The two-stage process consists of a thermophilic H2-reactor and a mesophilic CH4-reactor, achieved the highest hydrogen and methane yields of 76.8 mL/g-VS and 147.6 mL/g-VS at hydraulic retention times (HRTs) of 0.8 d and 6 d, respectively. The co-digestion process in this study required much less external alkalinity to maintain the pH values than sole food waste digestion in the literature. Compared with the single-stage mesophilic methane AD process, the two-stage AD system had better performance on operation stability, biogas and energy yields, organics removal and chemical oxygen demand (COD) conversion at high organic loading rates (OLRs). According to the TA-cloning analysis, the dominant bacteria in H2-reactor was closely related to Clostridium sp. strain Z6 and species Thermoanaerobacterium thermosaccharolyticum. The dominant methanogens in two-stage and single-stage CH4-reactor were recognized as acetotrophic methanogens and hydrogenotrophic methanogens, respectively. The presence of the genus Nitrososphaera in the two CH4-reactors might contribute to the low NH4+-N concentration in digestate and low CO2 content in biogas.

Enhanced biomethane recovery from fat, oil, and grease through co-digestion with food waste and addition of conductive materials

In this study, the effect of conductive additives on co-digestion of fat, oil, and grease (FOG) and food waste (FW) was evaluated. Initially, biochemical methane potential (BMP) test was conducted for optimization of mixing ratio of FW and FOG. The optimal methane production (800 L (kg VS)−1) was obtained from co-digestion of 70% FW + 30% FOG (w/w), which was 1.2 times and 12 times of that obtained from mono-digestion of FW and FOG, respectively. This optimal mixing ratio was used for subsequent fed-batch studies with the addition of two conductive additives, granular activated carbon (GAC) and magnetite. The addition of GAC significantly shortened the lag phase (from 7 to 3 d), reduced accumulation of various volatile fatty acids (VFAs), and enhanced methane production rate (50–80% increase) compared to the control and magnetite-amended bioreactor. Fourier transformation infrared (FTIR) analysis suggested that the degradation of lipids, protein and carbohydrates was the highest in GAC amended reactor, followed by magnetite and control reactors. GAC addition also enriched more abundant and diverse bacteria and methanogens than control. Magnetite addition also showed similar trends but to a lesser degree. The substantial enrichment of syntrophic LCFA β-oxidizing bacteria (e.g. Syntrophomonas) and methanogenic archaea in the GAC-amended bioreactor likely attributed to the superior methanogenesis kinetics in GAC amended bioreactor. Our findings suggest that the addition of GAC could provide a sustainable strategy to enrich kinetically efficient syntrophic microbiome to favor methanogenesis kinetics in co-digestion of FW and FOG.

Anaerobic Codigestion of Food Waste and Polylactic Acid: Effect of Pretreatment on Methane Yield and Solid Reduction

Food waste and biopolymers, plastics derived from plants, are unexploited sources of energy when discarded in landfills without energy recovery. In addition, polylactic acid (PLA) and food waste have complimentary characteristics for anaerobic digestion; both are organic and degrade under anaerobic conditions. Lab-scale reactors were set up to quantify the solubilization of pretreated amorphous and crystalline PLA. Biochemical methane potential (BMP) assays were performed to quantify CH4 production from both treated and untreated PLA in the presence of food waste and anaerobic digested sludge. Amorphous and crystalline PLA reached near-complete solubilization at 97% and 99%, respectively, when alkaline pretreatment was applied. The PLA that received alkaline treatment produced the most of CH4 throughout the run time of 70 days. The PLA without treatment resulted in 54% weight reduction after anaerobic digestion. Results from this study show that alkaline pretreatment has the greatest solid reduction of PLA and maximum production of CH4 when combined with food waste and anaerobic digested sludge.

Biogas production from high solids anaerobic co-digestion of food waste, yard waste and waste activated sludge

High-solids anaerobic co-digestion (HS-AcD) has the potential to recover energy and reduce environmental impacts of the organic fraction of municipal solid waste and waste activated sludge. We investigated the impact of substrate to inoculum (S/I) ratios, alkalinity sources (sodium bicarbonate and oyster shells), co-substrate mixing ratios and inoculum acclimation on HS-AcD of food waste, yard waste, and waste activated sludge using batch studies. Long-term HS-AcD performance was evaluated under the optimal conditions through a semi-continuous biodigester study with leachate recirculation. The digester with S/I = 1 using a mixture of crushed oyster shells and sodium bicarbonate as alkalinity sources had the highest methane yields (183 mL CH4/g VS). Addition of waste activated sludge to food waste and yard waste alleviated acidification (pH 6.86 ± 0.12) during the start-up period, which and improved digester stability. Mixtures with FW/YW/WAS = 0.8:1.7:0.5 had higher methane yields (134 ± 15 mL CH4/gVS) than mixtures with FW/YW/WAS = 1:1:1, but required a longer time (10 days) for self-recovery from volatile fatty acid inhibition. The use of an acclimated inoculum eliminated the lag time during start-up and produced 38% higher methane yield. In the semi-continuous biodigester, an average volatile solids reduction of 38% and methane yield of 186 mL/gVS was achieved. Improved performance in the semi-continuous biodigester compared with batch reactors was likely due to leachate recirculation, which can improve mass transfer of substrates to the microbial community. Digestate produced from HS-AcD of waste organics had a 1.7–2.3 fold higher nitrogen, similar phosphorous and 0.2–0.3 fold lower potassium content than commercially available bioorganic fertilizer.

Comparison of single-stage and two-stage anaerobic co-digestion of food waste and activated sludge for hydrogen and methane production

In this study, the co-digestion of food waste and activated sludge was evaluated in a two-stage anaerobic system and compared to the traditional single-stage process. The two-stage system was composed by two reactors connected in series able to perform the fermentative and the methanogenic phases separated. Experiments were carried out in semi-continuous mode under mesophilic conditions (37 °C). The two-stage technology achieved an overall improvement of the anaerobic performances. Results highlighted an increase in biogas production and volatile solids degradation of 26% and 9%, respectively. Considering the whole two-stage system, i.e. the sum of the biogas productions of the first and the second digester, these percentages increased up to 35.0%. Concerning gas quality, the two-stage system achieved a hydrogen rich biogas in the first fermentative reactor and an improvement of methane content in the second methanogenic digester. The average methane content shifted from 61.2% to 70.1%. The highest methane production of the two-stage process was due to improved substrate hydrolysis, with increased amounts of volatile fatty acids made readily available in the second stage.

Microbial communities in co-digestion of food wastes and wastewater biosolids

The effect of food waste (FW) co-digestion with wastewater biosolids (WWB) on microbial communities was investigated through running thirteen lab-scale digesters for 100 days at different operational conditions i.e. organic loading rates (2 and 4 kgCOD/m3·day), feed types (WWB and FW), and FW content (10%, 90%, 100%). Compared with mono-digestion of WWB, FW co-digestion enhanced biogas production by 13% and COD degradation rates by up to 101%. Among fermentative bacteria/acetogens, Syntrophomonas was the dominant genus in FW digesters in contrast to the dominance of Clostridium in WWB digesters. The predominant methanogen was Methanosarcina in FW digesters in contrast to Methanosaeta in WWB digesters. COD degradation rates and methane yields were well correlated with Bacteroidetes population. Methane production rate was well correlated with Clostridium for FW digesters, with syntrophs for WWB digesters, and with aceticlastic methanogens for both digesters. Synergism was associated with hydrolytic bacteria, Clostridium, Syntrophomonas, syntrophs, Methanosarcina, and Methanobacterium.

Food waste co-digestion in Germany and the United States: From lab to full-scale systems

Using food waste (FW) as a co-substrate in anaerobic digestion (AD) results in increased energy production, decreases in greenhouse gas emissions, and recycles the FW nutrients back to the land for producing crops. This research investigated food waste AD systems in the United States (US) and Germany (DE) that co-digested FW with dairy manure at the lab and full-scale. In DE, the post-consumer FW had 32–49% more CH4 potential (477–499 mLCH4/gVS) than maize and grass silage (368 and 331 mLCH4/gVS) and solid and liquid manure (243 and 91 mLCH4/gVS). Methane production in the full-scale DE system with 66% FW (by volume) was 882 m3CH4/d, which was 37% higher than the laboratory results due to the 86-day retention and 42 °C AD conditions in the field. The pre-consumer FW in the US had a similar CH4 potential (264–553 mLCH4/gVS), but due to the lack of heating in the full-scale system, 62% less CH4 was produced than the lab-based potential. While DE requires pasteurization of FW for AD and bans FW bans to landfills, the US does not have specific requirements for FW treatment in AD or federal FW landfill policies, with a few forthcoming FW bans in some municipalities and states. As FW diversion and utilization in AD systems is expected to grow, it is important to understand the effect of FW in biogas production and nutrient content, comparisons between lab and field-scale results, and the effect of policy on FW utilization.

A New Adjustment Strategy to Relieve Inhibition during Anaerobic Codigestion of Food Waste and Cow Manure

A new adjustment strategy (controlling temperature, pH, inoculum dose, and liquid supernatant replacement in different digestion stages) was used to relieve volatile fatty acid (VFA) inhibition during anaerobic codigestion of FW and CM. Three digestion stages and groups were designed: initial stage (on days 1–5 the temperature was 45 °C), the second stage (on days 6–10 the temperature was 35 °C and inoculum was supplied), and the third stage (on days 11–50 the temperature was 35 °C and liquid supernatant was replaced). Groups A, B, and C had initial inoculums of 0, 100, and 200 mL and were supplied inoculums of 200, 100, and 0 mL, respectively. Results showed that in the initial stage, Group A had the highest VFA concentration (876.54 mg/L) and the lowest pH (3.6). In the second and third stages, pH (~5.5 and ~7.5) and VFA concentrations showed no significant differences in all groups. The highest VFA concentration (3248 mg/L), volatile solid (VS) removal rate (49.72%), and total methane production (TMP) (10,959 mL), the shortest λ (19.92 d), and the T90% (39.25 d) were obtained in Group B (pH 8.5). Group C had the highest chemical oxygen demand (COD) removal rate (96.91%). Group A obtained the maximal TBP of 25,626 mL (pH 8.0).

Microbiome network analysis of co-occurrence patterns in anaerobic co-digestion of sewage sludge and food waste

Addition of food waste (FW) as a co-substrate in anaerobic digesters of wastewater treatment plants is a desirable strategy towards achievement of the potential of wastewater treatment plants to become energy-neutral, diverting at the same time organic waste from landfills. Because substrate type is a driver of variations in phylogenetic structure of digester microbiomes, it is critical to understand how microbial communities respond to changes in substrate composition and concentration. In this work, high throughput sequencing was used to monitor the dynamics of microbiome changes in four parallel laboratory-scale anaerobic digesters treating sewage sludge during acclimation to an increasing amount of food waste. A co-occurrence network was constructed using data from 49 metagenomes sampled over the 161 days of the digesters' operation. More than half of the nodes in the network were clustered in two major modules, i.e. groups of highly interconnected taxa that had much fewer connections with taxa outside the group. The dynamics of co-occurrence networks evidenced shifts that occurred within microbial communities due to the addition of food waste in the co-digestion process. A diverse and reproducible group of hydrolytic and fermentative bacteria, syntrophic bacteria and methanogenic archaea appeared to grow in a concerted fashion to allow stable performance of anaerobic co-digestion at high FW.

Three-stage anaerobic co-digestion of food waste and waste activated sludge: Identifying bacterial and methanogenic archaeal communities and their correlations with performance parameters

A three-stage anaerobic digester setup was configured and evaluated for enhanced methane production during co-digestion of food waste and waste activated sludge and the corresponding bacterial and methanogen communities were characterized. Results showed that the average methane yield (0.496 L/gVS) in the three-stage digester was 13–52% higher than that of one- and two-stage digesters. Compared to controls, an increase of 12–47% in volatile solids reduction was achieved in the three-stage digester (69.3 ± 6.7%). Bacterial phyla Proteobacteria, Firmicutes and Bacteroidetes dominated in one-, two- and three-stage digester while genera Pseudomonas, Tissierella, and Petrimonas were selectively enriched in the three-stage digester due to functional segregation. Taxonomic analysis identified 8 dominant methanogen genera, of which Methanosarcina, Methanosaeta, Methanobacterium and Methanolinea collectively accounted for 80%. With increasing OLR and digester stage number, the dominant methanogenic pathway shifted from hydrogenotrophic pattern to acetoclastic pattern and reached a final synergy of these two.

Methane Production Dynamics of Co-Digestion of Cow Manure and Food Waste Under Mesophilic Condition

Methane Production Dynamics of Co-Digestion of Cow Manure and Food Waste Under Mesophilic Condition

Integrating anaerobic co-digestion of dairy manure and food waste with cultivation of edible mushrooms for nutrient recovery

State-level policies in the New England region of the United States require diversion of organic materials away from landfills. One management option for food waste is anaerobic co-digestion with dairy manure. In addition to biogas, anaerobic digestion produces separated solid and liquid digestates. Solid digestates in the region are typically recycled as animal bedding before returning to the digester and liquids are used to fertilize local soils. Repeated land application of nutrients can contribute to eutrophication risk over time and alternative models are needed to convert digestates into valuable export products. We tested solid digestates derived from dairy manure and food waste as substrate ingredients in the cultivation of Pleurotus ostreatus. We show these materials can be used to offset non-local substrate ingredients while achieving mushroom yields comparable to commercial recipes. This strategy could help divert nutrients away from land adjacent to digesters and into safe, protein-rich food, while producing useful spent mushroom substrate.

Efficient resource valorization by co-digestion of food and vegetable waste using three stage integrated bioprocess

During two-stage (Acidogenesis-Methanogenesis) process, solid organics and gaseous by-products are usually left unused. To increase resource recovery efficiency, a three stage process (Hydrolysis/Acidogenesis-Methanogenesis-Composting) was designed. Initially, co-digestion of food waste (FW) and vegetable waste (VW) was carried out in Leach Bed Reactor (LBR) for hydrolysis and acidogenesis, followed by airlift reactor (ALR) for methanogenesis for 21 days using two different feed stocks [2:3 FW:VW~FVW; FW alone]. Off gas from LBR was diverted to ALR to enhance methane recovery. Results depicted that volatile fatty acids (VFA) and biohydrogen production was more for FW fed system, while methane production was higher in FVW fed system. Three different functional zones in three separate chambers significantly accelerated organic removal rate while gas diversion increased overall methane recovery. In third stage, residual solid organic matter from LBR was subjected to aerobic composting and compost with N (%): 2.90 & 2.76; C/N ratio: 18.2 & 20.8 for FVW and FW was recovered. The three-stage process has advantages of zero waste generation and overall process stability, accounting for resource efficient circular loop.

Hydrogen and Methane Production from Co-digestion of Food Waste and Chicken Manure

Hydrogen and Methane Production from Co-digestion of Food Waste and Chicken Manure