The present study aimed to characterize the sport fishing that occurs in the middle Negro River region. The data collection was carried out with the application of semi-structured questionnaires to sport fishing operators and sport fishers. Data were analyzed using descriptive statistics. A total of 142 questionnaires was applied. The results obtained from the questionnaires indicated that most of the operators are male (97.62%), whose ages ranging from 24 to 57 years old (44.09 ± 10.14 years old). The average duration of the season was six months, during which the companies operate in five different types of fishing tourism. The boat-hotel is the most adopted form of accommodation by operators, as well as being the oldest. Transport for the fishing trips is carried out in a greater quantity with semi-flat-nosed boats equipped with a 30-hp engine. Fishing operations are carried out depending on the type of operation, and trips last for seven days, with six fishing days. Twenty-two rivers were identified as being used for catche of Cichla temensis as the target species, and the main channel, the Aracá, Demeni and Cuiuni Rivers, was the most used.