Vasaguduchyadi Kwatha is a compound Ayurvedic formulation, indicated for the treatment of liver diseases, especially for Kamala (jaundice) and Panduroga (anemia). However, till date, no safety profile of this formulation has been reported; hence, in the present study, freshly prepared and market samples of Vasaguduchyadi Kwatha were evaluated for acute toxicity. Acute toxicity test was evaluated as per OECD 425 guidelines with 5 000 mg/kg as limit test in Wistar strain albino rats. Test formulations were administered to overnight fasted animals and parameters like body weight, behavioral changes, and mortality were assessed for 14 days. Hematological and biochemical parameters were assessed on 14th day. Results showed no significant changes in terms of behavioral changes, mortality, and body weight. Both the samples did not affect any of the hematological parameters. However, increase in blood urea level was observed. This study shows that both the samples of Vasaguduchyadi Kwatha are relatively safe up to the dose of 5 g/kg. However, further chronic toxicity evaluation is necessary to establish its safety profile on chronic administration.