Eggs are a source of many nutrients, including cholesterol and choline, thus there is uncertainty regarding the impact of egg intake on plasma lipids and the choline metabolite trimethylamine‐N‐oxide (TMAO). In particular, it has not been established whether there is a dose‐dependency between egg consumption and changes in plasma lipids and plasma TMAO concentrations. Therefore, the effects of increasing daily egg intake on biomarkers associated with cardiovascular disease (CVD) risk were evaluated in a young, healthy population. Thirty‐eight subjects (19 men/19 women, 24.1 ± 2.2 y, BMI 24.3 ± 2.5 kg/m2) were recruited for this 14‐week crossover intervention. Participants underwent a 2‐week washout period with no egg consumption (0 eggs/d), followed by the intake of 1, 2, and 3 eggs/d for 4 weeks each. Anthropometric data (weight, body mass index (BMI), waist circumference (WC)), blood pressure (BP), dietary records, and plasma biomarkers (lipids, glucose, liver enzymes, C‐reactive protein (CRP), choline, and TMAO) were assessed following each study period. BMI, WC, systolic BP, plasma glucose, triglycerides, total cholesterol, liver enzymes, and CRP were unchanged during the intervention. Diastolic BP decreased with increasing egg intake (p < 0.05). Intake of nutrients present in eggs (fat, cholesterol, choline, selenium, and vitamin D) increased with egg intake (p < 0.05) while carbohydrate and fiber intake and glycemic load decreased (p < 0.05). Compared to 0 eggs/d, intake of 1 egg/d increased HDL cholesterol (HDL‐c) (p < 0.05); HDL‐c remained elevated with intake of 2–3 eggs/d. Compared to 0 eggs, Intake of 1 egg/d resulted in a 10.9% decrease in plasma LDL cholesterol (LDL‐c) (p < 0.05) while there was no difference in LDL‐c between intake of 0, 2, or 3 eggs/d. Intake of 1–3 eggs/d also resulted in a decreased LDL‐c/HDL‐c ratio (p < 0.01) when compared to 0 eggs/d. Lastly, plasma choline increased (p < 0.0001) in a dose‐dependent manner with egg intake but remained within established normal ranges, while fasting plasma TMAO did not change. These data indicate that intake of up to 3 eggs/d results in an overall beneficial effect on biomarkers associated with CVD risk in a healthy population, as documented by increased HDL‐c and plasma choline and reductions in the LDL‐c/HDL‐c ratio, in combination with unchanged plasma LDL‐c and TMAO concentrations.Support or Funding InformationThis research was funded by an award to MLF by the Esperance Family Foundation and a grant awarded to DMD by the Egg Nutrition Center.