Stress Reactivity In Children Research Articles

Parental buffering in the context of poverty: positive parenting behaviors differentiate young children's stress reactivity profiles.

Experiencing poverty increases vulnerability for dysregulated hypothalamic-pituitary-adrenal (HPA) axis functioning and compromises long-term health. Positive parenting buffers children from HPA axis reactivity, yet this has primarily been documented among families not experiencing poverty. We tested the theorized power of positive parenting in 124 parent-child dyads recruited from Early Head Start (Mage = 25.21 months) by examining child cortisol trajectories using five samples collected across a standardized stress paradigm. Piecewise latent growth models revealed that positive parenting buffered children's stress responses when controlling for time of day, last stress task completed, and demographics. Positive parenting also interacted with income such that positive parenting was especially protective for cortisol reactivity in families experiencing greater poverty. Findings suggest that positive parenting behaviors are important for protecting children in families experiencing low income from heightened or prolonged physiologic stress reactivity to an acute stressor.

Maternal stress during pregnancy and stress reactivity in children: A meta-analysis

Maternal stress during pregnancy and stress reactivity in children: A meta-analysis

Cognitive-affective strategies and cortisol stress reactivity in children and adolescents: Normative development and effects of early life stress.

This study examined cognitive-affective strategies as predictors of hypothalamic-pituitary-adrenal (HPA) axis responses to a social-evaluative stressor in adolescence as compared to late childhood as a function of early life experiences. Participants included 159 children (9-10years) and adolescents (15-16years) divided into two groups based on early care experiences: non-adopted youth raised in their birth families (n=81) and post-institutionalized youth internationally adopted from orphanage care (n=78). Youth completed a version of the Trier Social Stress Test modified for use with children and reported on their trait emotion regulation and coping strategies. Children reported more use of suppression and disengagement than adolescents, while adolescents reported more engagement coping strategies. Non-adopted and post-institutionalized youth did not differ in reported strategies. Cognitive reappraisal predicted higher cortisol reactivity in non-adopted children and adolescents, and was not associated with reactivity in the post-institutionalized group. This study has implications for efforts aimed at promoting self-regulation and adaptive stress responses during the transition to adolescence for both typically developing children and children who experienced adverse early care.

Influence of Acute Physical Activity on Stress Reactivity in Obese and Normal Weight Children: A Randomized Controlled Trial

Objective: Physical activity (PA) may influence acute stress reactivity in children differently depending on their weight. This randomized controlled trial investigated the impact of acute PA and of BMI status (overweight/obese (OB/OW) and normal weight (NW) on stress reactivity. Method: 50 prepubertal children (24 OW/OB and 26 NW) were randomly assigned to the PA or sedentary arm (SED) for 30 min followed by a stress task. Salivary cortisol, blood pressure (BP), and heart rate (HR) were measured. Results: An interaction effect between the randomization arms and weight status on salivary cortisol was found after the stress task (p = 0.04). Cortisol increased in the SED, but not in the PA arm (p = 0.004 for differences in time course) of NW children. Time course did not differ between both arms in OW/OB children (p = 0.7). OW/OB SED children had a flat cortisol course, and levels were reduced compared to the NW SED or the OW/OB PA children (p ≤ 0.03). Systolic BP increased only in the SED arm (p = 0.01). HR was higher in the PA than in the SED arm during stress (p < 0.001) and showed different time courses (p = 0.006). Conclusion: PA impacted on acute stress reactivity and influenced stress reactivity differently in NW and OW/OB children.

Salivary cortisol and alpha-amylase diurnal profiles and stress reactivity in children with Attention Deficit Hyperactivity Disorder

There is growing evidence for dysregulation of the stress system in individuals with Attention Deficit Hyperactivity Disorder (ADHD). The stress system includes neuroanatomical and functional components that function in concert to maintain homeostasis and its main effectors are the hypothalamic-pituitary-adrenal (HPA) axis and the sympathetic/adrenomedullary nervous system (SNS). As stress system activity demonstrates a distinct circadian variation, we aimed to describe simultaneously, diurnal rhythms of both the HPA axis and the SNS in children with ADHD and a comparison group. Moreover, we attempted to investigate stress responses to a physical stressor, venipuncture, in both groups. Sixty-two prepubertal children with ADHD combined (ADHD-C) or inattentive (ADHD-I) type and 40 typically developing children provided saliva samples at six specific time points during a day, as well as before and 10 min after a scheduled morning venipuncture. Salivary cortisol and α- amylase were selected as reliable noninvasive biomarkers for HPA axis and SNS function and were measured in the samples obtained. Results revealed that children with ADHD-C had lower mean cortisol values both 30 min after awakening and at 18:00 h than controls (p = 0.002 and p = 0.018 respectively), as well as lower mean Cortisol Awakening Response (CAR) and Area Under the Curve for “wake to bed” period (AUCi) values of cortisol (p = 0.004 and p = 0.001, respectively). Also, mean CAR and cortisol AUCi were lower in children with ADHD-I than the control group (p = 0.034 and p = 0.038 respectively). Alpha-amylase measurements showed an increase over time (p < 0.001), which was similar in all three groups. Interestingly, α-amylase changes over time were correlated with the corresponding cortisol changes (p < 0.001). Venipuncture, elicited a significant increase only in α-amylase levels and more so in the control group (p = 0.003). These findings suggest a partial hypofunction of the stress system in children with ADHD.

Maternal immune and affiliative biomarkers and sensitive parenting mediate the effects of chronic early trauma on child anxiety.

Chronic early trauma alters children's stress reactivity and increases the prevalence of anxiety disorders; yet the neuroendocrine and immune mechanisms underpinning this effect are not fully clear. Animal studies indicate that the mother's physiology and behavior mediate offspring stress in a system-specific manner, but few studies tested this external-regulatory maternal role in human children exposed to chronic stress. We followed a unique cohort of children exposed to continuous wartime trauma (N = 177; exposed; N = 101, controls; N = 76). At 10 years, maternal and child's salivary immunoglobulin A (s-IgA) and oxytocin (OT), biomarkers of the immune and affiliation systems, were assayed, maternal and child relational behaviors observed, mother and child underwent psychiatric diagnosis, and child anxiety symptoms assessed. War-exposed mothers had higher s-IgA, lower OT, more anxiety symptoms, and their parenting was characterized by reduced sensitivity. Exposed children showed higher s-IgA, more anxiety disorders and post traumatic stress disorder, and more anxiety symptoms. Path analysis model defined three pathways by which maternal physiology and behavior impacted child anxiety; (a) increasing maternal s-IgA, which led to increased child s-IgA, augmenting child anxiety; (b) reducing maternal OT, which linked with diminished child OT and social repertoire; and (c) increasing maternal anxiety, which directly impacted child anxiety. Our findings, the first to measure immune and affiliation biomarkers in mothers and children, detail their unique and joint effects on children's anxiety in response to stress; highlight the relations between chronic stress, immune activation, and anxiety in children; and describe how processes of biobehavioral synchrony shape children's long-term adaptation.

Empathy networks in the parental brain and their long-term effects on children's stress reactivity and behavior adaptation

Parental empathy is a key component of sensitive parenting that supports children's social adaptation throughout life. Consistent with a two dissociable network perspective on empathy, we measured within- and between-network integrity of two empathy-related networks in the parental brain as predictors of children's social outcomes across the first six years of life. We focused on two empathy networks; embodied simulation, which supports parents' capacity to resonate with infant state and emotions and implicates cingulo-insulary structures, and mentalizing, which underpins parents' theory-of-mind and mental attributions via prefrontal-temporo-parietal circuit. We followed 87 first-time parents across the first six years of family formation, including heterosexual and homosexual parents. In infancy, parents' brain response to own versus unfamiliar infant stimuli was imaged; in preschool, children's cortisol production and emotion regulation were assessed; and at six years, children's behavior problems were reported. Parents' intra- and inter- network integrity increased when viewing their own infant compared to unfamiliar infant, suggesting that attachment stimuli increase network coherence in the parental brain. Functional connectivity within the parent's embodied simulation network in infancy predicted lower child cortisol production while inter-network connectivity among the embodied simulation and mentalizing networks was associated with more advanced child emotion regulation skills in preschool and lower internalizing problems at six years. Children's emotion regulation capacities mediated the link between inter-network integrity in the parental brain and internalizing symptoms. Our findings, the first to demonstrate that integrity of empathy-related networks in the parental brain shape children's long-term stress reactivity and emotional adaptation, highlight the brain component of the parental empathy attribute, suggest that increased coherence within the "parental caregiving network" marks a key feature of parent-infant attachment, and contribute to discussion on biobehavioral mechanisms underpinning the cross-generation transmission of human stress reactivity and sociality.

What is the effect of yoga compared to a physical education class on stress reactivity for sixth-grade students?

This study was interested in the physiological responses of children to stressful stimuli or stress reactivity. As the authors noted, stress reactivity in children has been related to important hea...

Parental Anxiety Prospectively Predicts Fearful Children's Physiological Recovery from Stress.

Parental anxiety confers risk for the development of an anxiety disorder in children, and this risk may be transmitted through children's stress reactivity. Further, some children may be more vulnerable to reactivity in the presence of parent factors such as anxiety. In this study, we examined whether parents' anxiety symptoms prospectively predict school-aged children's physiological reactivity following stress, assessed through their electrodermal activity (galvanic skin response) during recovery from a performance challenge task, and whether this varies as a function of children's temperamental fearfulness. Parents and their children (N=68) reported on their anxiety symptoms at Time 1 of data collection, and parents characterized the extent to which their children had fearful temperaments. At Time 2 children completed the performance challenge and two recovery tasks. Greater parental anxiety symptom severity at Time 1 predicted children's higher electrodermal response during both recovery tasks following the failure task. Further, these effects are specific to children with medium and high fearful temperament, whereas for children low in fearfulness, the association between parent anxiety and child reactivity is not significant. Findings provide additional evidence for the diathesis-stress hypothesis and are discussed in terms of their contribution to the literature on developmental psychopathology.

Disparate plasma cortisol concentrations in sexually abused female children from Johannesburg, South Africa

A growing body of research indicates that a bidirectional response to a stressor may occur in maltreated children and may be associated with later life psychopathology. However, few studies have investigated stress reactivity in children when they first present to a sexual abuse clinic. Thus, in order to evaluate whether HPA axis dysregulation would be evident at first presentation to a sexual abuse clinic in young girls (n=26), between the ages of 6–12 years old, blood samples were obtained immediately following examination at a forensic sexual abuse clinic and from the matched control group of children (n=14; 10.1±0.8) immediately following a bone density scan. Stratification of the sexually abused group into those children who were reportedly abused by a stranger and had no other family stressors (n=15, 10.4±1.8) and those children whose parents reported abuse of the child by a stranger and other family stressors (n=11; 9.5±1.8) revealed differences in stress reactivity. Plasma concentrations, of the children from the forensic clinic, were significantly increased in children who reported abuse by a stranger only (322.3±117.4nmol/l) and significantly decreased in children whose histories indicated sexual abuse by a stranger and other family stressors (149.6±39.7nmol/l) when compared to the control group (225.5±47.5nmol/l). In conclusion, following sexual abuse and a secondary stressor, the forensic examination, there is evidence of divergent cortisol responses in the stratified clinical group of children.

Does the arrival index predict physiological stress reactivity in children

Knowledge about children's stress reactivity and its correlates is mostly based on one stress task, making it hard to assess the generalizability of the results. The development of an additional stress paradigm for children, that also limits stress exposure and test time, could greatly advance this field of research. Research in adults may provide a starting point for the development of such an additional stress paradigm, as changes in salivary cortisol and alpha-amylase (sAA) over a 1-h pre-stress period in the laboratory correlated strongly with subsequent reactivity to stress task (Balodis et al., 2010, Psychoneuroendocrinology 35:1363–73). The present study examined whether such strong correlations could be replicated in 9- to 11-year-old children. Cortisol and sAA samples were collected from 158 children (83 girls) during a 2.5-h visit to the laboratory. This visit included a 1-h pre-stress period in which children performed some non-stressful tasks and relaxed before taking part in a psychosocial stress task (TSST-C). A higher cortisol arrival index was significantly and weakly correlated with a higher AUCg but unrelated to cortisol reactivity to the stressor. A higher sAA arrival index was significantly and moderately related to lower stress reactivity and to a lower AUCi. Children's personality and emotion regulation variables were unrelated to the cortisol and sAA arrival indices. The results of this study do not provide a basis for the development of an additional stress paradigm for children. Further replications in children and adults are needed to clarify the potential meaning of an arrival index.

Cortisol Reactivity in Two-Year-Old Children Prenatally Exposed to Methamphetamine

Until now, the functioning of the hypothalamic-pituitary-adrenal (HPA) axis in children with prenatal methamphetamine exposure (PME) had been unexamined. Previous research indicates that prenatal exposure to stimulant drugs is associated with dose-response alterations in neural growth and connectivity and consequent neurobehavioral deficits. In addition, children of drug-using parents are at an increased risk for exposure to chronic postnatal stress. In this preliminary study, we examined the associations of PME and postnatal environmental stress with cortisol stress reactivity in children with PME. Participants were 2-year-old children (N = 123; 55.3% male) with PME from a multicenter longitudinal Infant, Development, Environment, and Lifestyle Study. Saliva samples were obtained before and after a stress-inducing separation task. Hierarchical multiple regression analyses examined prenatal drug exposure, methodological and postnatal stress covariates, and interactions between levels of PME and postnatal stress. Mild to moderate potential for child physical abuse moderated increased cortisol reactivity in high exposed children with PME. Blunted cortisol reactivity was associated with caregiver's postnatal alcohol use, child's behavioral dysregulation, and the interaction between higher levels of PME and caregiver's psychopathology. Consistent with the known effects of stimulant drugs and chronically stressful environments on the HPA axis and, thus, the toxic stress and allostatic load phenomena, our results imply that elevated PME may be associated with alterations in the programming of the HPA axis reflecting hyperactivity, which under significant and chronic environmental stress then may become hypoactive.

Determinants of physiological and perceived physiological stress reactivity in children and adolescents.

AimsAbnormal physiological stress reactivity is increasingly investigated as a vulnerability marker for various physical and psychological health problems. However, studies are inconsistent in taking into account potential covariates that may influence the developing stress system. We systematically tested determinants (individual, developmental, environmental and substance use-related) of physiological and perceived physiological stress reactivity. We also examined the relation between physiological and perceived physiological stress reactivity.MethodIn a stratified sample of 363 children (7–12 years) and 344 adolescents (13–20 years) from the general population, we examined cortisol, heart rate, respiratory sinus arrhythmia and perceived physiological stress reactivity to a psychosocial stress procedure.ResultsUsing multivariate linear regression models, we found that individual, developmental, environmental and substance use-related factors were related to each of the stress response indices. These determinant factors were different for each of the stress reactivity indices, and different in children versus adolescents. Perceived physiological stress reactivity predicted cortisol reactivity in adolescents only. All other relations between perceived physiological and physiological stress reactivity were not significant.ConclusionsAs physiological stress variables are often examined as vulnerability markers for the development of health problems, we maintain that it is essential that future studies take into consideration factors that may account for found relations. Our study provides an overview and indication of which variables should be considered in the investigation of the relation between physiological stress indices and illness.

Anxiety symptom severity differentiates HPA acute stress reactivity in children

Anxiety symptom severity differentiates HPA acute stress reactivity in children

Testosterone is involved in mediating the effects of prenatal stress in male guinea pig offspring

A link exists between stress during pregnancy and altered hypothalamic–pituitary–adrenal (HPA) activity and behaviour in children. In the guinea pig, male offspring born to mothers that were exposed to stress during pregnancy demonstrated increased anxiety, basal cortisol levels and decreased testosterone concentrations. Testosterone is known to inhibit HPA function and anxiety behaviours. Therefore, we hypothesized that restoring plasma testosterone would ameliorate the differences observed in HPA function and behaviour. Pregnant guinea pigs were exposed to a stressor during the period of rapid fetal brain growth (prenatal stress, PS) or left undisturbed (control, C). Behaviour in an open-field and prepulse inhibition (PPI) of the acoustic startle reflex (ASR) was assessed in juvenile offspring. In adulthood, male offspring were divided into four groups: Control + sham gonadectomy (GDX), control + GDX + testosterone replacement, PS + sham GDX and PS + GDX + testosterone. Male offspring were retested in the open-field and PPI. Basal HPA activity was also assessed. As juveniles, PS males exhibited significantly lower ASR (P < 0.05) and elevated PPI. In adulthood, PS male offspring exhibited significantly decreased PPI (P < 0.02) and this was reversed by administration of testosterone. We also found that adult PS offspring exhibited significantly less activity in the open-field (P < 0.05) and administration of testosterone increased ambulatory activity in PS animals. Basal plasma adrenocorticotrophin hormone (ACTH) levels were significantly greater in PS animals and there was a trend towards reversal by administration of testosterone in PS males. In conclusion, prenatal stress results in male guinea pig offspring that exhibit age-dependent differences in ambulatory activity, sensorimotor gating and HPA activity. In adulthood, the behavioural changes are reversed by replacement of plasma testosterone.

Neurobiological and behavioral stress reactivity in children prenatally exposed to tobacco

This study examined neurobiological and behavioral stress reactivity in children who had been prenatally exposed to tobacco. Neurobiological stress reactivity was measured using salivary cortisol and alpha-amylase levels at five different time points throughout a stressful neuropsychological test session, which involved a competition against a videotaped opponent. Participants (mean age: 10.6 years, SD 1.3) were 14 prenatally exposed (PE) children, 9 children with disruptive behavior problems (DBD), and 15 normal controls (NC). For cortisol responses, no significant differences between the three groups were observed. Normal controls, however, had significantly higher alpha-amylase levels than PE-children throughout the test session, and their alpha-amylase levels also increased throughout the session, whereas these remained low and stable for PE-children. Alpha-amylase levels and trajectory of PE-children were similar to those observed for DBD-children. PE-children also showed significantly increased behavioral stress reactivity compared to NC-children, and neurobiological and behavioral stress reactivity were inversely related in PE-children, again similar to what was observed for DBD-children. These results support the hypothesis that prenatal smoking may lead to long-lasting neurobiological and behavioral changes in exposed offspring.

Maternal distress in early life predicts the waist-to-hip ratio in schoolchildren.

We report on life course stress determinants of overweight in children, using data from the longitudinal follow-up of the nested case-control arm of the SAGE (study of asthma genes and the environment) birth cohort in Manitoba, Canada. Waist and hip measurements were obtained during a clinic visit at age 9-11 years. Multiple linear regression was conducted to determine the relationship between the waist-to-hip ratio and maternal smoking during pregnancy, postpartum maternal distress and stress reactivity in children (cortisol, cortisol-DHEA [dihydroepiandrostrenone] ratio quartiles) following a clinic stressor at age 8-10 years. We found waist-to-hip risk at age 9-11 years to be elevated among boys and girls whose mothers had experienced distress in the postnatal period. This association varied by gender and asthma status. In healthy girls, postpartum distress increased waist-to-hip ratio by a factor of 0.034 (P < 0.01), independent of the child's stage of puberty and adrenarche, cortisol-DHEA ratio and duration of exclusive breastfeeding. Among girls with asthma, maternal smoking during pregnancy was associated with an increased waist-to-hip ratio, if the mother also experienced distress in the postpartum period (0.072, P = 0.038). Among asthmatic boys, an association between maternal distress and waist-to-hip ratio was evident at the highest cortisol-DHEA ratios. Stress-induced changes to leptin and infant over-eating pathways were proposed to explain the postnatal maternal distress effects. Drawing on the theories of evolutionary biology, our findings underscore the significance of postnatal stress in disrupting hypothalamic-pituitary-adrenal axis function in infants and increasing risk for child overweight.

P.4.d.001 Influence of peptide bioregulator on hormones in therapy of patients with organic disorders

A growing body of research indicates that a bidirectional response to a stressor may occur in maltreated children and may be associated with later life psychopathology. However, few studies have investigated stress reactivity in children when they first present to a sexual abuse clinic. Thus, in order to evaluate whether HPA axis dysregulation would be evident at first presentation to a sexual abuse clinic in young girls (n = 26), between the ages of 6–12 years old, blood samples were obtained immediately following examination at a forensic sexual abuse clinic and from the matched control group of children (n = 14; 10.1 ± 0.8) immediately following a bone density scan. Stratification of the sexually abused group into those children who were reportedly abused by a stranger and had no other family stressors (n = 15, 10.4 ± 1.8) and those children whose parents reported abuse of the child by a stranger and other family stressors (n = 11; 9.5 ± 1.8) revealed differences in stress reactivity. Plasma concentrations, of the children from the forensic clinic, were significantly increased in children who reported abuse by a stranger only (322.3 ± 117.4 nmol/l) and significantly decreased in children whose histories indicated sexual abuse by a stranger and other family stressors (149.6 ± 39.7 nmol/l) when compared to the control group (225.5 ± 47.5 nmol/l). In conclusion, following sexual abuse and a secondary stressor, the forensic examination, there is evidence of divergent cortisol responses in the stratified clinical group of children.

Emotion dysregulation and stress reactivity in children at risk for depression

Emotion dysregulation and stress reactivity in children at risk for depression

Protective effect of interval exercise on psychophysiological stress reactivity in children

Two studies determined whether interval exercise reduces children's stress reactivity. For Experiment 1 children completed interval exercise (n=14) or watched TV (n=14) for 25 min. After 20 min rest children completed a speech task. Speech-induced diastolic blood pressure (DBP) reactivity was dampened in the exercise group (p<.05). For Experiment 2 children (n=22) completed interval exercise-speech and TV-speech conditions on separate days. Physical activity was assessed by accelerometry and aerobic fitness estimated by submaximal exercise. DBP, systolic BP, and heart rate (HR) reactivity to the speech stressor were dampened (p<.05) after exercise compared to TV watching. Fitness was positively associated with HR reactivity. Interval exercise that mimics usual patterns of physically active play dampens cardiovascular reactivity to interpersonal stress.