Chicken Populations Research Articles

Analysis of Risk Factors and Antibiotic Resistance of Pathogenic Escherichia Coli Strains Isolated from Livestock Layer Chicken in Binjai, Indonesia

Binjai is one of the cities in North Sumatra Province Indonesia, with the second largest layer chicken population in North Sumatra. Studies on the use of antibiotics in layer chicken farms in North Sumatra, especially in Binjai, are still relatively minimal, so studies are still needed to find out the picture of antibiotic use and levels of antibiotic resistance. The aim of this study was to determine the level of resistance of pathogenic E. coli bacterial strains to antibiotics in layers of chicken farms in the city of Binjai and to find out which pathogenic E. coli bacterial strains O157:H7 are resistant type of antibiotic Tetracycline and Penicillin. The data were analyzed using a multivariable logistic regression model (SPSS). The results of antimicrobial resistance (AMR) testing against 9 types of antibiotics showed that the antibiotic Amoxicillin has the highest level of resistance. Based on the results of the analysis of risk factors for the occurrence of pathogenic E. coli strains in 120 isolates, there were possible causes for the occurrence of pathogenic E. coli at the research location, basically poor cage cleanliness and the provision of antibiotics as a preventative, through drinking water, so that the dosage were unmeasured and usage patterns were inappropriately monitored.

Simultaneous detection of infectious bronchitis virus and avian metapneumovirus genotypes A, B, and C by multiplex RT-qPCR assay in chicken tracheal samples in Ecuador.

Respiratory RNA viruses such as Infectious bronchitis virus (IBV) and Avian metapneumovirus (aMPV), which are characterized by generating both respiratory damage and adverse effects on reproductive organs, affect poultry production economically due to high mortality rate and decrease in egg production and quality. Particularly, aMPV has three genotypes that have been reported with greater frequency in chickens: aMPV-A, aMPV-B, and aMPV-C. The present study proposes the design of a multiplex RT-qPCR assay for the simultaneous diagnosis of the 3 genotypes of interest of aMPV and IBV, followed by testing of 200 tracheal samples of vaccinated chickens with respiratory symptoms and finally a phylogenetic analysis of the sequences found. The assay detected up to 1 copy of each viral genome. The standard curves showed an efficiency between 90 and 100% in the multiplex assay and inter- and intra-assay coefficients of variation of 0.363 and 0.459, respectively and inter- and intra-assay coefficients of variation of 0.363 and 0.459, respectively. 69.5% of samples were found positive alone or in coinfection. 114 samples were positive for IBV, 13 for aMPV-A and 25 for aMPV-B. RNA of aMPV-C was no detected. The most commonly found combination was aMPV-B and IBV within 6 samples, and the least common was aMPV-A and aMPV-B in coinfection in 2 samples. The assay was specific for amplification of the genomes of the studied respiratory viruses (IBV, aMPV-A, aMPV-B, aMPV-C) as no amplification was shown from other viral genomes (ChPV, CAstV, ANV, and FAdV) or from the negative controls. Partial genomic Sanger sequencing enabled to identify circulating vaccine-derived and wild-type strains of IBV and vaccine and vaccine-derived strains of aMPV-B. In conclusion, this newly developed multiplex RT-qPCR was shown to be able to detect individual infections as well as co-infections among the respiratory viruses investigated. It was demonstrated to be a reliable and efficient tool for rapidly and safely diagnosing these infections. Furthermore, this study represents the first report of aMPV strains in Ecuadorian poultry and demonstrates the circulation of aMPV-A, aMPV-B, and GI-13 IBV strains in unvaccinated chicken populations in the country. Thus, it highlights the importance of simultaneously identifying these pathogens in greater detail and on a regular basis in Ecuador.

EFFECT OF GENOTYPE, SEX AND AGE AND ITS ASSOCIATION BETWEEN BODY WEIGHTS AND BODY MEASUREMENTS IN INDIGENOUS CHICKEN POPULATION

A study was carried out to determine the effect of genotype, sex and age and its association between body weights and body measurements in indigenous chicken population. The parent stocks were sourced from the local markets within Dutsin-Ma and its environs. Each parental genetic group was separated into breeding groups of 1:10 cock to hen, respectively on a deep litter. Performance data of body weight (BW), chest girth (CG), body length (BL), shank length (SL), shank circumference (SL), wing length (WL) and keel length (KL), respectively of 120 progenies generated at 4, 8, 12, 16 and 20 weeks of age were analyzed using Statistical Analysis System (SAS, Version, 9.1). Generally, body increased with age. The overall mean obtained were 659.64 g, 20.93 cm, 22.12 cm, 8.00 cm, 11.93 cm, and 8.24 cm for body weight, chest girth, body length, shank length, wing length and keel length, respectively. The effect of genotype on body weight and body measurements were significant (P<0.05). The naked neck chickens had higher values for all the morphological traits, followed by normal and frizzle feathered birds, respectively. Males had significantly (P<0.05) higher body weights and body measurements than females. The phenotypic correlations among growth traits were positive moderate, high and significant (P<0.05). The estimates ranged from 0.49 to 0.73 between body weight and body measurements. Highest estimates were observed between body weight and chest girth (rp=0.73) in naked neck chicken. Correlations between sexes were generally high and positive and ranged from 0.61 to 0.77 and 0.56 to 0.70 in males and females, respectively. The highest (rp=0.77) positive coefficient was recorded between body weight and wing length in males and between body weight and body length (rp=0.71) in the females. This implies that selection for improvement in the linear body traits could lead to improvement in body weight of these chickens.

LncEDCH1 g.1703613 T>C regulates chicken carcass traits by targeting miR-196-2-3p

Single nucleotide polymorphisms (SNPs) are valuable genetic markers that can provide insights into the genetic diversity and variation within chicken populations. In poultry breeding, SNP analysis is widely utilized to accelerate the selection of desirable traits, improving the efficiency and effectiveness of chicken breeding programs. In our previous research, we identified an association between LncEDCH1 and muscle development. To further investigate its specific mechanism, we conducted SNP detection and performed genotyping, linkage disequilibrium, and haplotype analysis. Our research findings indicate that 16 SNPs in the LncEDCH1. Among these SNPs, g.1703497 C>T and g.1704262 C>T were significantly associated with breast muscle weight percentage, g.1703497 C>T and g.1703613 T>C were significantly associated with leg weight percentage, and g.1703497 C>T, g.1703589 T>C, g.1703613 T>C, g.1703636 C>A, g.1703768 T>C, g.1704079 C>T, g.1704250 T>C, g.1704253 G>A were significantly associated with skin yellowness. Two haplotype blocks composed of 6 SNPs that were significantly associated with wing skin yellowness, breast skin yellowness, full-bore weight, and carcass weight percentage. Furthermore, through dual-luciferase reporter assays, biotin-coupled miRNA pull-down assays, 5-ethynyl-2'-deoxyuridine (EDU) assays, immunofluorescence, and quantitative real-time polymerase chain reaction (qPCR), it has been confirmed that miR-196-2-3p inhibits the expression of LncEDCH1 directly by binding to LncEDCH1 g.1703613T>C, thereby achieving indirect regulation of muscle development. These findings provide valuable molecular markers for chicken molecular breeding and broaden our understanding of the regulatory mechanisms.

Implementasi Sistem Otomatisasi Monitoring Suhu, Kelembapan, dan Amonia pada Kandang Ayam Petelur Menggunakan Metode Fuzzy

The increase in demand for egg consumption and the high chicken population in Indonesia has not been matched by the maintenance of good chicken coops, this has led to a decrease in the quality of eggs produced. Then an automation system is needed in the chicken coop to control temperature, humidity and ammonia levels. Automatic control is by spraying cooling water and prebiotic liquids. The time in the spraying process is calculated using the fuzzy method to determine the spraying decision. The spraying data on the system is compared with manual fuzzy calculations using confusion matrix calculations to get the accuracy value of spraying on the system. The calculation results show that although the precission in water spraying and prebiotic watering reaches 100%, the recall is still low. This shows that improvements are still needed to improve the accuracy of the tool. However, this automation system still has the potential to assist laying hen farmers in optimizing the cage environment, maintaining the health of laying hens, and increasing productivity effectively and efficiently. With this system, it is hoped that the quality of life and welfare of laying hens can be improved, as well as help farmers manage cages more efficiently.

Genetic Architecture of Abdominal Fat Deposition Revealed by a Genome-Wide Association Study in the Laying Chicken.

Fat has a high energy density, and excessive fatness has been recognized as a problem for egg production and the welfare of chickens. The identification of a genetic polymorphism controlling fat deposition would be helpful to select against excessive fatness in the laying hen. This study aimed to estimate genomic heritability and identify the genetic architecture of abdominal fat deposition in a population of chickens from a Dongxiang blue-shelled local breed crossbred with the White Leghorn. A genome-wide association study was conducted on abdominal fat percentage, egg production and body weights using a sample of 1534 hens genotyped with a 600 K Chicken Genotyping Array. The analysis yielded a heritability estimate of 0.19 ± 0.04 for abdominal fat percentage; 0.56 ± 0.04 for body weight at 72 weeks; 0.11 ± 0.03 for egg production; and 0.24 ± 0.04 for body weight gain. The genetic correlation of abdominal fat percentage with egg production between 60 and 72 weeks of age was -0.35 ± 0.18. This implies a potential trade-off between these two traits related to the allocation of resources. Strong positive genetic correlations were found between fat deposition and weight traits. A promising locus close to COL12A1 on chromosome 3, associated with abdominal fat percent, was found in the present study. Another region located around HTR2A on chromosome 1, where allele substitution was predicted to be associated with body weight gain, accounted for 2.9% of phenotypic variance. Another region located on chromosome 1, but close to SOX5, was associated with egg production. These results may be used to influence the balanced genetic selection for laying hens.

Effect of two different dietary endo-1,4-β-xylanases on growth performance, intestinal histomorphology, caecal microbial population and short-chain fatty acid composition of broiler chickens

Effects of two different dietary xylanases on broiler performance and gut integrity were determined. Totally, 7600-d-old Ross-308 chicks were used for 41 d. Chicks were randomly assigned to 4 dietary treatments with 10 replicates (95 males, 95 females per replicate); they were: positive control (PC) based on Ross 308 nutrient specification; negative control (NC) with less ME (80 kcal/kg), crude protein and amino acids compared to PC; NC + AO with addition of xylanase derived from Aspergillus oryzae (AO); NC + TB with addition of xylanase derived from Trichoderma bisset (TB). Birds fed on PC diet significantly (p < 0.05) had higher final body weight (BW) and body weight gain (BWG) compared to other treatments. The final BWG and feed conversion ratio (FCR) were not fully compensated with the addition of the enzymes compared to PC, and FCR was found significantly (p < 0.05) higher in birds fed on diets NC + AO and NC + TB. However, dietary xylanase significantly improved FCR, villus surface area and total goblet cell number in NC + AO and NC + TB birds compared to NC. Xylanase supplementation significantly increased the concentrations of branched-chain fatty acid (BCFA) and total caecal short-chain fatty acid (SCFA) (p < 0.05). Furthermore, both xylanases increased lactobacillus count (p < 0.05). Dietary treatments had no effect on carcase parameters and mortality rate. In conclusion, along with improving performance parameters, the inclusion of xylanase enzymes with nutrient matrix value in broilers’ diet may ameliorate intestinal morphology, stimulate caecal microflora and increase caecal SCFA concentrations, but nutrient contribution of both enzymes seems overestimated.HIGHLIGHTSDietary xylanase could not compensate the final BWG and FCR compared to positive control.Dietary xylanase increased villus surface area and total goblet cell number.Dietary xylanase increased the concentrations of branched-chain fatty acids and total caecal short-chain fatty acid.

Major Histocompatibility Complex Region and Diversity of the Local Chicken Populations In Niger

Introduction: The major histocompatibility complex (MHC) of chicken is highly polymorphic, and it is linked to several disease resistance or susceptibility traits. Therefore, the current study aimed to analyze the genetic diversity in the MHC region of Nigerien local chicken (Dourgou, Goggori, Kolonto, Tchagara, Gouzou-gouzou, and Popular) using a high polymorphic microsatellite marker named LEI0258 to determine the diversity of chickens kept at the four agroecological zones in Niger. Materials and methods: A total of 601 chickens from six local Nigerien chickens were sampled. By capillary electrophoresis using LEI0258 marker, 403 samples with different fragment sizes were randomly chosen and sequenced. Results: The findings indicated 80 different alleles ranging in size from 181 to 474 bp. A total of 22 new alleles and 39 private alleles (that existed in only one breed) were detected. The alleles 309, 295, and 193 were the most predominant in the Nigerien local chicken population. Nine polymorphisms were observed along the LEI0258 sequence, including three in the upstream (one indel and two Single Nucleotide Polymorphism [SNP]), one in the repeat region at the last R12 (SNP), and five in the downstream (two indels and three SNPs). Conclusion: The chickens are not clustering according to their agroecological zone of origin. They are randomly distributed across the four investigated agroecological zones. The information found in this study is invaluable in breeding and conservation programs associated with several disease resistance or susceptibility traits.

Weighted single-step GWAS identified candidate genes associated with carcass traits in a Chinese yellow-feathered chicken population

Carcass traits in broiler chickens are complex traits that are influenced by multiple genes. To gain deeper insights into the genetic mechanisms underlying carcass traits, here we conducted a weighted single-step genome-wide association study (wssGWAS) in a population of Chinese yellow-feathered chicken. The objective was to identify genomic regions and candidate genes associated with carcass weight (CW), eviscerated weight with giblets (EWG), eviscerated weight (EW), breast muscle weight (BMW), drumstick weight (DW), abdominal fat weight (AFW), abdominal fat percentage (AFP), gizzard weight (GW), and intestine length (IL). A total of 1,338 broiler chickens with phenotypic and pedigree information were included in this study. Of these, 435 chickens were genotyped using a 600K single nucleotide polymorphism chip for association analysis. The results indicate that the most significant regions for 9 traits explained 2.38% to 5.09% of the phenotypic variation, from which the region of 194.53 to 194.63Mb on chromosome 1 with the gene RELT and FAM168A identified on it was significantly associated with CW, EWG, EW, BMW, and DW. Meanwhile, the 5 traits have a strong genetic correlation, indicating that the region and the genes can be used for further research. In addition, some candidate genes associated with skeletal muscle development, fat deposition regulation, intestinal repair, and protection were identified. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses suggested that the genes are involved in processes such as vascular development (CD34, FGF7, FGFR3, ITGB1BP1, SEMA5A, LOXL2), bone formation (FGFR3, MATN1, MEF2D, DHRS3, SKI, STC1, HOXB1, HOXB3, TIPARP), and anatomical size regulation (ADD2, AKT1, CFTR, EDN3, FLII, HCLS1, ITGB1BP1, SEMA5A, SHC1, ULK1, DSTN, GSK3B, BORCS8, GRIP2). In conclusion, the integration of phenotype, genotype, and pedigree information without creating pseudo-phenotype will facilitate the genetic improvement of carcass traits in chickens, providing valuable insights into the genetic architecture and potential candidate genes underlying carcass traits, enriching our understanding and contributing to the breeding of high-quality broiler chickens.

Impact of Glycetin Injection on Productive Traits in local Iraqi Chickens

This study aimed to investigate the impact of implementing Glycetin by injection on the productive features of local Iraqi chickens. 100 female hens and 20 roosters from a local Iraqi chicken population, at age 26 weeks. The sample was divided into three groups, in addition to a control group. Each experimental group had 25 female hens, with the same procedure being performed for each individual hen, and 20 roosters, which were reared separately for the purpose of artificial insemination. The avian specimens were housed in separate enclosures, and the experimental conditions were allocated as follows: the initial condition (Control Group) did not receive any injections, whereas conditions T2, T3, and T4 were administered with varying doses of Glycetin at concentrations of 5, 10, and 15 milligrams per kilogram of body weight, respectively. The results showed improve significant (P<0.05) many aspects such as egg production rate, cumulative egg count, egg mass, egg weight, and feed conversion ratio.

АНАЛИЗ СПЕКТРА ПАТОГЕНОВ ПАРАЗИТАРНОЙ, БАКТЕРИАЛЬНОЙ И ВИРУСНОЙ ЭТИОЛОГИИ В ПРОМЫШЛЕННОМ ПТИЦЕВОДСТВЕ. ПРАКТИЧЕСКИЙ ОПЫТ

Practical experience in diagnosing infectious and parasitic diseases of poultry is presented using the example of an industrial flock of laying hens kept on the territory of a poultry farm. An assessment is made of the spectrum of pathogens of parasitic, bacterial and viral etiology circulating in a particular poultry farm. Clinical, pathological, parasitological and serological studies were carried out on samples of material taken from the poultry population. The immune status of the chicken population was monitored in relation to pathogens of infectious and parasitic diseases and the effectiveness of specific prevention against infectious and parasitic diseases in poultry was analyzed.

A novel goose-origin Tembusu virus exhibits pathogenicity in day-old chicks with evidence of direct contact transmission

In late 2020, an outbreak of Tembusu virus (TMUV)-associated disease occurred in a 45-day-old white Roman geese flock in Taiwan. Here, we present the identification and isolation of a novel goose-origin TMUV strain designated as NTU/C225/2020. The virus was successfully isolated using minimal-pathogen-free duck embryos. Phylogenetic analysis of the polyprotein gene showed that NTU/C225/2020 clustered together with the earliest isolates from Malaysia and was most closely related to the first Taiwanese TMUV strain, TP1906. Genomic analysis revealed significant amino acid variations among TMUV isolates in NS1 and NS2A protein regions. In the present study, we characterized the NTU/C225/2020 culture in duck embryos, chicken embryos, primary duck embryonated fibroblasts, and DF-1 cells. All host systems were susceptible to NTU/C225/2020 infection, with observable lesions. In addition, animal experiments showed that the intramuscular inoculation of NTU/C225/2020 resulted in growth retardation and hyperthermia in day-old chicks. Gross lesions in the infected chicks included hepatomegaly, hyperemic thymus, and splenomegaly. Viral loads and histopathological damage were displayed in various tissues of both inoculated and naïve co-housed chicks, confirming the direct chick-to-chick contact transmission of TMUV. This is the first in vivo study of a local TMUV strain in Taiwan. Our findings provide essential information for TMUV propagation and suggest a potential risk of disease outbreak in chicken populations.

Demand And Supply Of Chicken Egg In South Sulawesi

This research aims to analyze the factors that influence the demand and supply of chicken eggs in South Sulawesi. The research method used was a literature study with data sourced from Central Statistics Agency data and other sources in the form of scientific journals, scientific articles, and other valid information from print and digital media. Data analysis was carried out using descriptive analysis techniques. Based on the data obtained, it is known that the development of the purebred chicken population and demand for chicken eggs in South Sulawesi from 2018-2022 fluctuates and tends to increase. along with population growth in South Sulawesi. The production of purebred chicken eggs in South Sulawesi over the last 5 years has fluctuated. The number of egg production increased significantly by 38% from 2018 to 2019 and decreased in 2020 and 2021. Then the number of egg production increased again by 6.49% from 2021 to 2022. This happened because consumer demand for purebred chicken eggs increased. Thus, it can be said that people need purebred chicken eggs to meet their needs for animal protein sources. Factors that influence the demand and supply of broiler eggs in South Sulawesi are fluctuating egg prices, producer profits, raw materials (feed and seeds), demand, and disease outbreaks.

Phenotypic characterization and genetic diversity of indigenous chickens of Jordan in comparison with native and commercial breeds ‎for conservation and breeding purposes

Indigenous chickens of Jordan are facing extinction and need genetic conservation because they were unable to commercially compete in poultry industry because of low genetic ability ‎compared to commercial layers. The study aimed to investigate phenotypic and genetic diversity ‎of village chickens in the Jordan using discriminant analyses procedures to provide a basis for ‎sustainable genetic conservation and utilization program to overcome any possible extinction. The ‎sampled chicken population of 578 one-year old chickens (125 males and 433 females) was ‎phenotypically characterized for 15 biometric and plumage traits from major cities of the three ‎regions; Middle, North, and South. The traits variations within and between breeds were detected ‎statistically by stepwise discriminant and canonical-discriminant of uni- and multivariate analyses. ‎The results showed the sampled population as village chickens in Jordan is comprised of ‎indigenous (Baladi) breed (85%) and few exotic and commercial breeds. The breeds were distinct ‎and differentiated based on phenotypic traits indicating high genetic variability. The major ‎phenotypic traits that showed significant power to differentiate breeds were comb type, body ‎weight, comb size, earlobe color, wattle size, face color and breast size in males and comb type and ‎size, body weight, face and breast size, leg color and wattle size in females. Recent and past ‎crossings, admixture or migration from exotic and commercial breeds were noted. Moreover, low ‎levels of phylogeographic structure were observed across the studied breeds. In conclusion, there is need to conserve the indigenous breed in situ and in vivo for its adaptive gene pool in coming ‎days of persisted climate change and diseases threats.

Recombinant chimpanzee adenovirus expressing spike protein protects chickens against infectious bronchitis virus

Infectious bronchitis (IB) is an acute and highly contagious disease caused by avian infectious bronchitis virus (IBV), resulting in significant economic losses in the global poultry industry. In this study, we utilized a replication-incompetent adenovirus vector derived from chimpanzees for the first time to express the S gene of IBV. The adenovirus was successfully rescued and demonstrated convenient production, good growth performance, and stability on HEK293 A cells. Morphologically, the recombinant adenovirus (named PAD-S) appeared normal under transmission electron microscopy, and efficient expression of the exogenous gene was confirmed through immunofluorescence analysis and immunoblotting. Administration of PAD-S via ocular and nasal routes induced a strong immune response in the chicken population, as evidenced by specific antibody and cytokine measurements. PAD-S was unable to replicate within chickens and showed low pre-existing immunity, demonstrating high safety and environmental friendliness. The robust immune response triggered by PAD-S immunization effectively suppressed viral replication in various tissues, alleviating clinical symptoms and tissue damage, thus providing complete protection against viral challenges in the chicken population. In conclusion, this study successfully developed an IBV candidate vaccine strain that possesses biosafety, high protective efficacy, and ease of production.

Prospects for Free-Range Chicken Development in Meeting Market Demand in South Sulawesi

Free-range chicken farming has promising prospects for development, whether we admit it or not, consumer appetite for free-range chicken is still very high, this can be seen from population growth and demand for free-range chicken which increases from year to year. So it is not surprising that the culinary business using free-range chicken as its raw material is mushrooming and increasing rapidly, especially in tourist destinations in Indonesia. It seems to be a breath of fresh air for beginner breeders and for those who want to try free-range chicken farming. This research aims to analyze the prospects, potential, and demand for free-range chickens in Indonesia. The increase in the free-range chicken population in the last 4 years, namely in 2019 as many as 30 038 625 birds, until in 2022 it will be 30 996 054 chickens, means that products in the form of free-range chicken meat and eggs also increase. The increase in the amount of production every year has not been able to meet the consumption needs of the population in South Sulawesi. Production of free-range chicken meat in 2022 will be 5,590,90 tons, while the amount of consumption required will be 63,697,438 tons. Likewise, free-range chicken egg products in 2022 will amount to 20 069.95 tons, while the need for eggs will reach 175 033 tons. The high demand for consumption shows potential business prospects in developing free-range chicken businesses in South Sulawesi Province.

Prevalence and Molecular Characteristics of FAdV-4 from Indigenous Chicken Breeds in Yunnan Province, Southwestern China.

Fowl adenovirus-induced hepatitis-pericardial effusion syndrome outbreaks have been increasingly reported in China since 2015, resulting in substantial economic losses to the poultry industry. The genetic diversity of indigenous chicken results in different immune traits, affecting the evolution of these viruses. Although the molecular epidemiology of fowl adenovirus serotype 4 (FAdV-4) has been well studied in commercial broiler and layer chickens, the prevalence and genetic characteristics of FAdV-4 in indigenous chickens remain largely unknown. In this study, samples were collected from six indigenous chicken breeds in Yunnan province, China. FAdV-positive samples were identified in five of the six indigenous chicken populations via PCR and 10 isolates were obtained. All FAdVs belonged to serotype FAdV-4 and species FAdV-C. The hexon, fiber, and penton gene sequence comparison analysis demonstrated that the prevalence of FAdV-4 isolates in these chickens might have originated from other provinces that exported chicks and poultry products to Yunnan province. Moreover, several distinct amino acid mutations were firstly identified in the major structural proteins. Our findings highlighted the need to decrease inter-regional movements of live poultry to protect indigenous chicken genetic resources and that the immune traits of these indigenous chickens might result in new mutations of FAdV-4 strains.

Assessing the conservation impact of Chinese indigenous chicken populations between ex-situ and in-situ using genome-wide SNPs

Conservation programs require rigorous evaluation to ensure the preservation of genetic diversity and viability of conservation populations. In this study, we conducted a comparative analysis of two indigenous Chinese chicken breeds, Gushi and Xichuan black-bone, using whole-genome SNPs to understand their genetic diversity, track changes over time and population structure. The breeds were divided into five conservation populations (GS1, 2010, ex-situ; GS2, 2019, ex-situ; GS3, 2019, in-situ; XB1, 2010, in-situ; and XB2, 2019, in-situ) based on conservation methods and generations. The genetic diversity indices of three conservation populations of Gushi chicken showed consistent trends, with the GS3 population under in-situ strategy having the highest diversity and GS2 under ex-situ strategy having the lowest. The degree of inbreeding of GS2 was higher than that of GS1 and GS3. Conserved populations of Xichuan black-bone chicken showed no obvious changes in genetic diversity between XB1 and XB2. In terms of population structure, the GS3 population were stratified relative to GS1 and GS2. According to the conservation priority, GS3 had the highest contribution to the total gene and allelic diversity in GS breed, whereas the contribution of XB1 and XB2 were similar. We also observed that the genetic diversity of GS2 was lower than GS3, which were from the same generation but under different conservation programs (in-situ and ex-situ). While XB1 and XB2 had similar levels of genetic diversity. Overall, our findings suggested that the conservation programs performed in ex-situ could slow down the occurrence of inbreeding events, but could not entirely prevent the loss of genetic diversity when the conserved population size was small, while in-situ conservation populations with large population size could maintain a relative high level of genetic diversity.

POPULATION DOCUMENTATION OF PELUNG CHICKEN IN FIVE SUB DISTRICTS IN GARUT REGENCY

Pelung chicken is a local Indonesian chicken originating from Cianjur. The Pelung population data is still not known with certainty. This study aims to obtain the total and structure of population, determine the effective population size (Ne), and the rate of inbreeding (∆F) in Pelung chickens in five districts in Garut Regency. This research was conducted from May to June 2022 in Cikajang, Pasirwangi, Bayongbong, Samarang, and Cigedug Districts. The study used, quantitative descriptive methods and data were obtained by direct interview techniques in which a total of respondents were interviewed. The results of the study showed that the total population of Pelung chickens at the research location was 1,867 Pelung chickens with 452 birds in district of Cikajang, 316 of Pasirwangi, 555 of Bayongbong, 194 of Samarang, and 350 of Cigedug. The total number of effective population sizes at the study site was 465 individuals. The inbreeding rate per generation in five sub-districts was 0.1%, with the highest inbreeding rates in Samarang 0.82%, followed by Cigedug 0.78%, Pasirwangi 0.54%, Cikajang 0.42%, and Bayongbong 0.39%.

Analisis Jiwa Kewirausahaan terhadap Keberhasilan Usaha Peternak Plasma Ayam Ras Pedaging di Gianyar-Bali

The increase in the population of broiler chickens is in line with the increasing public demand for chicken and the improving economy of Bali in the new normal period of Covid-19. The entrepreneurial spirit plays an important role in the progress of the broiler business. This study aims to analyze: the level of entrepreneurial spirit of farmers; which indicators of the entrepreneurial spirit and characteristics of farmers have the most dominant influence on the success of broiler business; and the influence of entrepreneurial spirit and farmer's characteristics on the success of broiler business in Gianyar Regency, Bali Province. The study was conducted using a purposive sampling method, simple random sampling was conducted on 40 farmers using a structured questionnaire to obtain primary research data. Data were analyzed using Partial Least Square (PLS). The results showed that most of the respondents had a fairly high entrepreneurial spirit. The entrepreneurial spirit of breeders is proven to be influenced by self-confidence, initiative, has a motive for achievement, has a leadership spirit, and likes, where liking challenges is the strongest indicator to reflect this variable. While the individual characteristics of breeders are influenced by age, education, skills, number of chicken ownership, and knowledge. The number of chicken ownership is the most dominant reflecting indicator.