Chelated Form Research Articles

Fertigation of Ajwain (Trachyspermum ammi L.) with Fe-Glutamate Confers Better Plant Performance and Drought Tolerance in Comparison with FeSO4

Shortage of fresh water limits crop yield. Different ways including the use of chemicals are being employed for the improvement in yield through induction of plant performance. In the present study, ajwain plants grown under water stress and normal irrigation conditions were fertigated with Fe-chelated glutamate (Fe-Glu), as a foliar spray for the induction of plant performance in comparison with FeSO4. Water shortage adversely affected the plant growth and seed yield, associated with decreased uptake of water and nutrients, along with perturbations in different physio-biochemical attributes. On the other hand, Fe-Glu and FeSO4 fertigation improved plant performance under water stress and normal irrigation conditions. Fe-Glu and FeSO4 fertigation ameliorated the adverse effects of water stress on biomass and seed production, improved water and nutrients uptake, increased the accumulation of essential amino acids, leaf chlorophyll and carotenoids, and reduced the lipid peroxidation due to the induction of antioxidative mechanisms. Fertigation of Fe-Glu and FeSO4 also improved Fe uptake and conferred better mobility and availability of Fe for plants when applied in chelated form. Overall, a significant improvement in ajwain performance under water stress and normal irrigation conditions was recorded due to the fertigation of Fe-Glu as compared with FeSO4.

Growth regulator and microfertilizers on Sage (Salvia officinalis L.) and Greater Burdock (Arctium lappa L.): effectiveness of complex application

Relevance.Meeting the needs of the pharmaceutical industry with domestic medicinal raw materials is relevant. Methods.The aim of the study is to determine the effectiveness application by growth regulator, organomineral and microfertilizers on Sage (Salvia officinalis L.) and Greater Burdock (Arctium lappa L.) in conditions of Non-chernozem zone of the Russian Federation. The experimental part of the work was performed in Institute of Medicinal and Aromatic Plants in 2010-2011 and 2016-2017 by field experiments. On burdock we tested fertilizer Siliplant (0.5 l / ha): the first treatment was in the rosette phase, the second - after 20 days. On sage studied binary mixture of organomineral fertilizer EcoFys (1 l / ha) with fertilizer Zitovit (0.5 l / ha; applied in phase regrowth of plants) with following foliar application by complex microfertilizers Siliplant (0.5 l / ha) and growth regulator Zircon (40 ml / ha). The solution consumption is 300 l / ha.Results. The effectiveness of chelated form micro-fertilizers and organomineral fertilizers applied on various medicinal plants was shown. It has been established, that complex application by EcoFys+Zitovit (I treatment) and microfertilizer Siliplant with growth regulator Zircon (II treatment) can increase the productivity of Sage: yield of grass raised by 23%, roots by 40%. Similar results were obtained on the Greater Burdock culture – treatment of plants with microfertilizer Siliplant allowed to increase the yield of roots by 28%, leaves-by 24%.

Dependence of yield and quality of soybean grain on macro and micro fertilizers against the background of liming aftereffect

The article presents the results of research performed in a field stationary experiment on leached chernozem. In soy sowings of the Mageva variety, studied was the effectiveness of joint and separate application of various doses of mineral fertilizers and chelated form of microfertilizers (ZhUSS-2) in variants with soil previously limed in 0.5 and 1.0 h.a. The analysis of crop yield showed that the value of this indicator was significantly influenced only by mineral fertilizers, which provided an increase from 0.05 to 0.37 t/ha. as compared to the variant without fertilizers (0.89 t/ha, LSD05 0.05 t/ha). An increase in the level of nitrogen nutrition from 45 to 60 kg a. i. / ha did not contribute to a significant increase in soybean productivity and reduced the payback of 1 kg of a. i. nitrogen by grain from 7.11 to 5.44 kg. The content of crude protein in soybean grain significantly increased as the result of the aftereffect of liming of the soil in 0.5 h.a. by 0.79 % (LSD05 0.33 %), of ZhUSS-2 preparation use ˗ by 0.19 % (LSD05 0.16 %) and of mineral fertilizers use – by 0.26-4.02 % (LSD05 0.22%). When full mineral fertilizer was applied, the concentration of crude fat decreased by 0.53-0.62 abs. %. Considering unfavorable weather conditions for sowing soybean crops on leached chernozem it is possible to recommend the application of mineral fertilizers in doses of N30P50K80 and N45P50K80 together with treatment of crops with ZhUSS-2 preparation.

Influence of additive components with separate introduction of antagonistic components on gastrointestinal microorganization and microelements content in mouse blood serum

Taking into account the data on higher biological availability of chelate compounds of microelements in comparison with their inorganic salts, a liquid feed additive for cattle with the content of fat-soluble vitamins A, D, E and diacetophenonyl selenide (DAFS 25) in the form of stable microemulsion and chelates of manganese, iron, cobalt, and zinc was developed. The biochemical and physiologically antagonistic components of the additive are divided into two separate liquid mixtures for separate, alternating ingestion on white laboratory mice, histological methods evaluated the effect of different doses of the additive on the micro organization of the components of the stomach wall, small and large intestine in different terms of the experiment. The additive was introduced into animals individually daily per o s during one month in doses of 0.06, 0.12, 0.3, 0.6 and 1.2 g/kg. The control animals received an equivalent amount of physiological solution. The availability of trace elements of the developed product is estimated by analysis of blood serum on the zinc, iron, manganese, cobalt content by atomic-adsorption spectrometry. Examination bodies and blood for analysis were selected at the time of slaughter in accordance with generally accepted requirements. The dosage of the additive, which has the most effective influence on the growth of the concentration of injected micronutrients in the serum of mice in comparison with the control, was determined. It was found that the components of the additive have the most pronounced effect on zinc and iron content in the dose of 0.3 g/kg.

UV Photochemistry of Acetylacetaldehyde Trapped in Cryogenic Matrices.

The broad band UV photochemistry of acetylacetaldehyde, the hybrid form between malonaldehyde and acetylacetone (the two other most simple molecules exhibiting an intramolecular proton transfer), trapped in four cryogenic matrices, neon, nitrogen, argon, and xenon, has been studied by IRTF spectroscopy. These experimental results have been supported by B3LYP/6-311G++(2d,2p) calculations in order to get S0 minima together with their harmonic frequencies. On those minima, we have also calculated their vibrationally resolved UV absorption spectra at the time-dependent DFT ωB97XD/6-311++G(2d,2p) level. After deposition, only the two chelated forms are observed while they isomerize upon UV irradiation toward nonchelated species. From UV irradiation effects we have identified several nonchelated isomers, capable, in turn, of isomerizing and fragmenting, even if this last phenomenon seems to be most unlikely due to cryogenic cages confinement. On the basis of these findings, we have attempted a first approach to the reaction path of electronic relaxation. It appeared that, as with acetylacetone, the path of electronic relaxation seems to involve triplet states.

Detection and imaging of gadolinium accumulation in human bone tissue by micro- and submicro-XRF

Gadolinium-based contrast agents (GBCAs) are frequently used in patients undergoing magnetic resonance imaging. In GBCAs gadolinium (Gd) is present in a bound chelated form. Gadolinium is a rare-earth element, which is normally not present in human body. Though the blood elimination half-life of contrast agents is about 90 minutes, recent studies demonstrated that some tissues retain gadolinium, which might further pose a health threat due to toxic effects of free gadolinium. It is known that the bone tissue can serve as a gadolinium depot, but so far only bulk measurements were performed. Here we present a summary of experiments in which for the first time we mapped gadolinium in bone biopsy from a male patient with idiopathic osteoporosis (without indication of renal impairment), who received MRI 8 months prior to biopsy. In our studies performed by means of synchrotron radiation induced micro- and submicro-X-ray fluorescence spectroscopy (SR-XRF), gadolinium was detected in human cortical bone tissue. The distribution of gadolinium displays a specific accumulation pattern. Correlation of elemental maps obtained at ANKA synchrotron with qBEI images (quantitative backscattered electron imaging) allowed assignment of Gd structures to the histological bone structures. Follow-up beamtimes at ESRF and Diamond Light Source using submicro-SR-XRF allowed resolving thin Gd structures in cortical bone, as well as correlating them with calcium and zinc.

Эффективный метод управления продукционным процессом винограда с использованием некорневого питания в аридных условиях Северо-Западного Прикаспия

Abstract. Scientific novelty and practical significance. The research area is relevant due to the fact that in viticulture the use of new water-soluble complex fertilizers in chelated form is not yet widespread due to their insufficient knowledge. In this regard, it became necessary to determine their influence on the basic vital processes of grape plants in arid conditions. The purpose of the research is to identify the effect of foliar nutrition by “Plantafol” and “Boroplus” fertilizers on economically valuable grape indicators in the arid conditions of the North-Western Caspian during irrigation. Methods. Experience is two-factor. Factor A – varieties: Kodryanka (early ripe), Rizamat (mid-ripening), Moskovskiy (late ripening). Factor B: option 1 – control (water treatment); option 2 – “Plantafol;” option 3 – “Boroplus”; option 4 – “Plantafol” + “Boroplus”. The experience was laid and statistical data processing was carried out according to the method of B. A. Dospekhov. The counts and observations were carried out according to the method of M. A. Lazarevskiy. The mass fraction of sugars and titratable acidity in the juice of berries was found by the method of biochemical study of plants A. I. Ermakova. The content of vitamin C in grapes was determined by the guidelines for determining the quality of plant products. Results. The maximum yield increase for all varieties was provided by the combined use of “Plantafol” and “Boroplus” fertilizers. The variety Kodryanka has 6.6 t/ha for control. The variety Rizamat has 6.7 t/ha for control. Foliar top dressing contributed to a significant increase in the mass fraction of sugars in all varieties. The use of “Boroplus” in its pure form and together with “Plantafol” positively affected the vitamin C content in berries. The sugar acid index of the varieties “Rizamat” (29.8) and Moskovskiy (28.1) had optimal values for the variant without the use of fertilizers, and the varieties Kodryanka (25.3) – for the variant with their combined use.

CAN COPPER AND ZINC IN DIFFERENT CHEMICAL FORMS CAN IMPROVE IRON DEFICIENT IN PHASEOLUS PLANT

This study was aimed to compare between the effects of different chemical forms of Zn and cupper[ionic forms (CuSO4), (Zn SO4) and chelated forms of [Cu Zn (II) HEDTA and Cu (II) HEDTA], whereas, HEDTA is N-(hydroxyethyl) ethylenediamine triacetic acid, applied at micromolar concentrations in the nutrient solution] of Phaseolus Vulgaris plants grown hydroponically under conditions of iron deficiency (- Fe) were investigated. Plant variants (– Fe + 2 µM Cu2+) and (– Fe+ 20 µM Zn2+) with extremely strong chlorosis were examined for investigations to take after the recuperation of leaf greening after treatment with Cu(II)HEDTA created leaf greening in the two variations, particularly strong for the recently which created leaf, as it appeared with chlorophyll estimations. Changes of plasma membrane reductase movement (PMRA) in roots after treatment with ionic or chelated copper were followed in (+Fe) and (– Fe) plants. The results show the increment of ferric-chelate reductase action (with substrate of Fe (III) HEDTA). Then, the cupric-chelate Cu (II) HEDTA, connected at similar level in arrangements with (– Fe) plants, kept up the high encouragement of plasma membrane ferric-chelate reductase activity. It can be concluded that the treatment with Cu (II) HEDTA enhanced the development and root plasma membrane reductase activity (PMRA) and additionally iron deficiency reactions of phaseolus plants. Regard to cell compounds increase, measurements of 20 μM of Zn altogether developed the action of the protein superoxide dismutase and peroxidase.

Functionalized-AgNPs for Long-Term Stability and Its Applicability in the Detection of Manganese Ions

In this study, silver nanoparticles (AgNPs) were functionalized by various molecules, including sodium borohydride (NaBH4), polyhexamethylene biguanide hydrochloride (PHMB), and Tween 80 to investigate the long-term stabilization of AgNPs in an aqueous dispersion. PHMB-functionalized silver nanoparticles (AgNPs/PHMB) exhibited better stability than others and could be stored at ambient temperature for at least 180 days. In addition to creating stabilization based on the electrostatic repulsion, the use of PHMB helped to increase the degree of stability of the colloidal AgNPs for a long time owing to strong interactions between Ag atoms on AgNPs with nitrogen (N) positions in PHMB molecules. The formed bond led to improving maintenance ability of the electrostatic repulsion layer among independent nanoparticles. The applicability of the as-prepared AgNPs/PHMB was also examined for Mn2+ detection via a colorimetric approach. The calibration curve was found to be linear over the range of 0–100 mM with a correlation coefficient of 0.97. The amine groups of PHMB brought out a cooperative effect to form of ion-templated chelation with Mn2+, which caused the aggregation of AgNPs/PHMB. This suggested that the AgNPs/PHMB could be used as a potential probe in the detection of Mn2+ ions. More importantly, the long-term stability of AgNPs/PHMB paved a great promising path to provide many further solutions for the producer in practical applications.

Effects of GLDA, MGDA, and EDTA chelating ligands on Pb sorption by montmorillonite

In this study, the effects of the new biodegradable chelating ligands, i.e., methylglycinediacetic acid (MGDA) and L-glutamic acid-N,N-diacetic acid (GLDA), were compared with that of ethylenediaminetetraacetic acid (EDTA) on lead (Pb) sorption by montmorillonite (MMT) at equimolar Pb:ligand ratios. The results of PHREEQC simulations indicated that Pb was predominantly present in the form of negatively-charged chelates with significantly different sorption behaviors compared to that of the Pb2+ cations. The time-dependent sorption of the Pb species on MMT was biphasic, with an initial fast phase occurring within 3 h followed by a slower one reaching equilibrium after approximately 12 h. The analysis of equilibrium data revealed that both capacity and affinity of MMT for Pb sorption were decreased in the presence of the chelating ligands. The moderating potential of ligands on Pb sorption decreased in the order of EDTA ≫ MGDA > GLDA in accordance with complex stability constants. The FE-SEM/EDX, ATR-FTIR, and XRD evidence indicated that Pb and Pb complexes were adsorbed, most likely through physical forces, on both external planar and edge surfaces of the MMT. In conclusion, GLDA and MGDA represented lower ability than EDTA to depress Pb2+ adsorption on MMT, and hence, lower potential to mobilize Pb in soils and sediments where MMT is an important mineral sorbent.

Composites of Biopolymers and ZnO NPs for Controlled Release of Zinc in Agricultural Soils and Timed Delivery for Maize

Zinc (Zn) micronutrient deficiency is a widespread problem affecting crops worldwide. Unlike conventional ionic fertilizers (Zn as salt or chelated forms), Zn-based engineered nanomaterials (ENMs) ...

Long-Term Evaluation of Gadolinium Retention in Rat Brain After Single Injection of a Clinically Relevant Dose of Gadolinium-Based Contrast Agents.

PurposeThe aim of this study was to investigate the presence and chemical forms of residual gadolinium (Gd) in rat brain after a single dose of Gd-based contrast agent.MethodsFour groups of healthy rats (2 sacrifice time-points, n = 10/group, 80 rats in total) were randomized to receive a single intravenous injection of 1 of the 3 Gd-based contrast agents (GBCAs) (gadoterate meglumine, gadobenate dimeglumine, or gadodiamide) or the same volume of 0.9% saline solution. The injected concentration was 0.6 mmol/kg, corresponding to a concentration of 0.1 mmol/kg in humans after body surface normalization between rats and humans (according to the US Food and Drug Administration recommendations). Animals were sacrificed at 2 washout times: 1 (M1) and 5 (M5) months after the injection. Total Gd concentrations were determined in cerebellum by inductively coupled plasma mass spectrometry. Gadolinium speciation was analyzed by size-exclusion chromatography coupled to inductively coupled plasma mass spectrometry after extraction from cerebellum.ResultsA single injection of a clinically relevant dose of GBCA resulted in the detectable presence of Gd in the cerebellum 1 and 5 months after injection. The cerebellar total Gd concentrations after administration of the least stable GBCA (gadodiamide) were significantly higher at both time-points (M1: 0.280 ± 0.060 nmol/g; M5: 0.193 ± 0.023 nmol/g) than those observed for macrocyclic gadoterate (M1: 0.019 ± 0.004 nmol/g, M5: 0.004 ± 0.002 nmol/g; P < 0.0001). Gadolinium concentrations after injection of gadobenate were significantly lower at both time-points (M1: 0.093 ± 0.020 nmol/g; M5: 0.067 ± 0.013 nmol/g; P < 0.05) than the Gd concentration measured after injection of gadodiamide. At the 5-month time-point, the Gd concentration in the gadoterate group was also significantly lower than the Gd concentration in the gadobenate group (P < 0.05). Gadolinium speciation analysis of the water-soluble fraction showed that, after injection of the macrocyclic gadoterate, Gd was still detected only in its intact, chelated form 5 months after injection. In contrast, after a single dose of linear GBCAs (gadobenate and gadodiamide), 2 different forms were detected: intact GBCA and Gd bound to soluble macromolecules (above 80 kDa). Elimination of the intact GBCA form was also observed between the first and fifth month, whereas the amount of Gd present in the macromolecular fraction remained constant 5 months after injection.ConclusionsA single injection of a clinically relevant dose of GBCA is sufficient to investigate long-term Gd retention in the cerebellar parenchyma. Administration of linear GBCAs (gadodiamide and gadobenate) resulted in higher residual Gd concentrations than administration of the macrocyclic gadoterate. Speciation analysis of the water-soluble fraction of cerebellum confirmed washout of intact GBCA over time. The quantity of Gd bound to macromolecules, observed only with linear GBCAs, remained constant 5 months after injection and is likely to represent a permanent deposition.

The effect of foliar fertilization on darkening of tuber flesh of selected potato cultivars

The aim of the study was to determine the effect of foliar fertilizers with the content of macro- and microelements used in the form of chelates on darkening of the tuber flesh of selected potato cultivars. The research was based on a 3-year (2013‒2015) field experiment carried out in Haczów (49°40'N, 21°54'E) on brown, slightly acidic soil. The experiment was based on the method of randomized sub-blocks, where the first order factor was foliar fertilization: Fortis Zn Mn + Fortis Aminotop (A), Fortis B Mo + Ferti Agro (B), Fortis Zn Mn + Fortis B Mo (C) and a standard object without foliar fertilization (0). Second-order factor included four edible potato cultivars of all earliness classes (‘Viviana’, ‘Vineta’, ‘Agnes’, ‘Jelly’). Significant differences in tuber darkening due to foliar application were observed only for tubers 24 h after cooking. The application of Fortis B Mo + Ferti Agro and Fortis Duotop Zn Mn + Fortis Aminotop fertilizers reduced the darkening of the apical part tuber to the greatest extent. Genetic factor differentiated the most the value of all examined traits. The cultivars tested showed varied response to foliar fertilizers.

ВЛИЯНИЕ ХЕЛАТНЫХ ФОРМ МЕДИ И ЦИНКА НА УРОЖАЙНОСТЬ И КАЧЕСТВО РАСТЕНИЙ РИСА В УСЛОВИЯХ КУБАНИ

ВЛИЯНИЕ ХЕЛАТНЫХ ФОРМ МЕДИ И ЦИНКА НА УРОЖАЙНОСТЬ И КАЧЕСТВО РАСТЕНИЙ РИСА В УСЛОВИЯХ КУБАНИ

Growth-stimulating role of chelates and organic acids

The effect of chelating forms of copper and zinc and organic acids on germination, germination energy, and morphometric parameters of Tríticum aestívum seedlings in an in vitro culture was experimentally established. The most effective growth regulators were identified, and their optimal doses for pre-sowing treatment of seeds were also established.

Influence of chelated forms of trace elements on the live weight and livability of rearing stock of broilers

Nowadays, organic forms of trace elements, which have a high bioavailability, and, as a consequence, have a beneficial effect on the body of animals and birds, are increasingly used in feeding. In this area, quite a lot of research has been carried out on the use of chelates in poultry feeding, but mainly for broiler chickens and laying hens. Therefore, the purpose of our study was the use of organic forms of trace elements such as iron, manganese, copper, zinc, and selenium in the diets of rearing stock of the parent flock of the ROSS cross. The experiment was carried out in the production conditions of the Novosafonovskaya poultry farm of Kuzbasskiy Broiler LLC and at the Small animal science department of the Altai State Agrarian University in 2020. The results of the experiment indicate that replacing 50% of the need for inorganic forms of iron, manganese, copper, zinc and selenium with organic analogs contributed to an increase in live weight, absolute and average daily gains of rearing stock. There was an increase in the uniformity of the flock in terms of live weight, both males and females. The crop percent of poultry in the experimental group was 3.2-4.6% higher due to a decrease not only in mortality, but also in culled chickens.

Synthesis of magnetic nanoparticles with an IDA or TED modified surface for purification and immobilization of poly-histidine tagged proteins.

Magnetic nanoparticles (MNPs) chelating with metal ions can specifically interact with poly-histidine peptides and facilitate immobilization and purification of proteins with poly-histidine tags. Fabrication of MNPs is generally complicated and time consuming. In this paper, we report the preparation of Ni(ii) ion chelated MNPs (Ni-MNPs) in two stages for protein immobilization and purification. In the first stage, organic ligands including pentadentate tris (carboxymethyl) ethylenediamine (TED) and tridentate iminodiacetic acid (IDA) and inorganic Fe3O4–SiO2 MNPs were synthesized separately. In the next stage, ligands were grafted to the surface of MNPs and MNPs with a TED or IDA modified surface were acquired, followed by chelating with Ni(ii) ions. The Ni(ii) ion chelated forms of MNPs (Ni-MNPs) were characterized including morphology, surface charge, structure, size distribution and magnetic response. Taking a his-tagged glycoside hydrolase DspB (Dispersin B) as the protein representative, specific interactions were confirmed between DspB and Ni-MNPs. Purification of his-tagged DspB was achieved with Ni-MNPs that exhibited better performance in terms of purity and activity of DspB than commercial Ni-NTA. Ni-MNPs as enzyme carriers for DspB also exhibited good compatibility and reasonable reusability as well as improved performance in various conditions.

The effect of metal ions on endogenous melanin nanoparticles used as magnetic resonance imaging contrast agents.

Melanin nanoparticles are of great importance in biomedicine. They have excellent affinity for metallic cations, especially paramagnetic ions, which has sparked interest in their application in the development of magnetic resonance imaging (MRI) contrast agents. In this work, we prepared ultrasmall water-soluble melanin nanoparticles, and investigated the binding properties of melanin toward different metal cations (Gd3+, Mn2+, Fe3+ and Cu2+), and compared their physicochemical properties and the MRI contrast enhancement ability in various metal chelated forms (MNP-PEG-M) in vitro and in vivo. We show that the saturation binding numbers of Gd3+, Mn2+, Fe3+ and Cu2+ per MNP-PEG were 49, 59, 69 and 62, respectively. MNP-PEG-Gd, MNP-PEG-Mn, MNP-PEG-Fe and MNP-PEG-Cu exhibited the maximum r1 relaxivities at the loading mass ratios of Gd3+ : MNP = 1 : 1, Mn2+ : MNP = 0.5 : 1, Fe3+ : MNP = 0.1 : 1 and Cu2+ : MNP = 0.1 : 1, corresponding to 49, 57, 54 and 51 chelated metals per MNP-PEG, respectively. The maximal per metal ion r1 relaxivity values were 61.9, 48.7, 11.1 and 9.7 mM-1 s-1 for MNP-PEG-Gd, MNP-PEG-Mn, MNP-PEG-Fe and MNP-PEG-Cu at 1.5 T, respectively. MNP-PEG-Gd and MNP-PEG-Fe presented larger sizes (6.9 nm and 5.8 nm) than MNP-PEG-Mn and MNP-PEG-Cu (3.4 nm and 3.7 nm), all featuring excellent solubility, high stability and ultrasmall size. A significant in vivo MRI signal enhancement in tissues was observed for all MNP-PEG-M after intravenous injection in mice, and these nanoparticles were excreted through renal and hepatobiliary pathways. In agreement with their r1 relaxivity values, MNP-PEG-Gd and MNP-PEG-Mn showed a significantly greater in vivo tissue maximum enhancement than MNP-PEG-Fe and MNP-PEG-Cu. This study could yield valuable insight into the development of a new class of MRI contrast agents.

Formation blue lupine productivity under different options of fertilization and seed treatment

Purpose. To increase the yield of blue lupine due to the intensification of the generative development of plants and maximum preservation of fruit elements by the phase of full ripeness by introducing the optimal dose of mineral fertilizers and foliar nutrition with a microfertilizer in chelated form at the optimum time, application of a bioinoculant and biofungicide. Methods. Field (to study the interaction of the research object with biotic and abiotic factors); morphophysiological (for biological control over the development of productivity elements at the stages of organogenesis); weight (to establish the parameters of the crop structure elements and determine the grain yield); statistical (statistical processing of research results). Results. The results of studies on the effects of different doses of mineral fertilizers, bioinoculant and biofungicide as well as foliar nutrition with microfertilizer in chelated form at different stages of organogenesis on the generative development of blue lupine plants of cultivar Peremozhets and grain yield are presented. The optimal dose of mineral fertilizers and the duration of plant foliar nutrition were determined. Conclusions. Application of the calculated dose of mineral fertilizers for the planned yield of grain of blue lupine of cultivar Peremozhets at the dose of 3.5 t/ha of mineral fertilizers (N68Р48К66), sowing with seeds treated with BTU-r bioinoculant (1 l/t) and MikoHelp biofungicide (1.0 l/t), foliar nutrition with Tropicel microfertilizer (0.3 kg/ha) at the second phase of plant organogenesis contributed to the intensification of their generative development (the number of flowers – 38.0 pcs., beans – 13.3 pcs./plant, which was 1.8 times higher than in the absolute control) as well as an increase by 39.3 % of the crop yield with a control indicator of 2.01 t/ha.

Effect of Trace Minerals and B Vitamins on the Proliferation/Cytotoxicity and Mineralization of a Gilthead Seabream Bone-Derived Cell Line.

Trace minerals and vitamins are known modulators of bone metabolism, and dietary optimization of these components may improve skeletal development and reduce the occurrence of skeleton deformities in farmed fish. As for larval stages, mineral and water-soluble vitamin nutrition requirementsare lacking in research efforts and knowledge is scarce. An in vitro cell system developed from gilthead seabream vertebra and capable of mineralization was used to assess the effect of B vitamins (thiamin and pyridoxine) and trace minerals (copper, manganese, and zinc in a sulfated and chelated form) on cell proliferation and extracellular matrix (ECM) mineralization. Dependent on dose, inhibition of cellular proliferation and/or cytotoxic effects was observed for all nutrients tested and LD50 valueswere determined: copper, 67.4-69.5 ppm; manganese, 20.9-29.8 ppm; zinc, 37.1-42.8 ppmin sulfated and chelated formrespectively; thiamin, 6273 ppm; pyridoxine, 14226 ppm. ECM mineralization was enhanced by mineral (dose and form dependent) and vitamin (dose dependent) supplementation, at non-toxic concentrationsbelow the determined LD50s. This in vitro work confirmed the mineralogenic action of trace minerals and water-soluble vitamins and provided valuable insights for subsequent in vivo nutritional trials.