Cheilomenes Sexmaculata Research Articles

Selection and validation of reference genes for quantitative expression analysis of regeneration-related genes in Cheilomenes sexmaculata by real-time qRT-PCR.

Regeneration is a fascinating phenomenon that has intrigued scientists for a long time. Cheilomenes sexmaculata (Fabricius), a zig-zag ladybird beetle, possesses a high capacity for limb regeneration. The molecular mechanics of the zig-zag ladybird beetle are under-explored. Current research trends are focused on uncovering functional genes associated with limb regeneration. Most of these investigations involve quantitative real-time PCR (qRT-PCR) for their rapid and accurate analysis of gene expression levels. Hence, a stable and suitable reference gene is required to normalize the gene expression data. In this study, five housekeeping genes were selected from the transcriptomics data (in-house unpublished data) of C. sexmaculata (Fabricius). The expression stabilities of the selected genes were evaluated under different time intervals post-amputation using geNorm, normFinder, and refFinder software. Actin was revealed to be the most stable housekeeping gene, along with elongation factor 2 and glyceraldehyde-3-phosphate dehydrogenase. A target gene named engrailed (an important segment-forming gene) was used to validate the selected reference genes. The expression levels were found to be consistent with the transcriptomics results. According to our study, actin, along with elongation factor 2 and glyceraldehyde-3-phosphate dehydrogenase, serve as the most stable reference genes and are suitable for regeneration-related research. This study is a groundbreaking effort to identify the most stable reference gene for limb regeneration in C. sexmaculata (Fabricius), and the findings can be applied to other related insect species.

Exploring the diversity of coccinellid species: A comprehensive study on polymorphism and species-specific male genitalia identification

In this study, the diversity of coccinellids along with their polymorphic forms is presented, by studying the structure of male genitalia and siphon. Out of the fourteen species of coccinellids listed, eleven species were predaceous on aphids. The male genitalia structures of the five most common species i.e., occinella septempunctata, Co. transversalis, Cheilomenes sexmaculata, Micraspis discolor and M. yasumatsui have been described and found to exhibit characteristic differentiating features. The melanic and non-melanic forms of Ch. sexmaculata, the most ubiquitous coccinellid in the Indian subcontinent, were dissected and shown to have similar male genitalia structures. Problem of misidentification due to polymorphic forms of coccinellid beetles can be mitigated by studying male genitalia and siphonal structures in addition to external morphological studies.

Ladybird Beetle Diversity in Natural and Human-Modified Habitats in the San Cristóbal Island, Galapagos, Ecuador.

This study investigates the species richness and distribution of ladybird beetles (Coccinellidae) across various habitats on San Cristóbal Island in the Galápagos Archipelago, Ecuador. Through extensive field surveys, we catalogued nineteen species, including four previously known species (two endemics, Psyllobora bisigma and Scymnobius scalesius, and two natives, Cycloneda sanguinea and Tenuisvalvae bromelicola). We also identified nine possibly native species reported for the first time in the Galapagos islands in this study or correspond to the first voucher specimens for the island. We collected three previously reported non-native species: Cheilomenes sexmaculata, Novius cardinalis, and Paraneda guticollis. Three species belonging to the genera Stethorus, Calloeneis, and Delphastus remain undetermined, pending further taxonomic analyses. Our findings reveal a rich and complex community with notable differences in species abundance and habitat preference. Endemic species were found to be particularly scarce and restricted mainly to crops undergoing forest regeneration and deciduous forests, emphasising their vulnerability and specialised habitat requirements. The native Cycloneda sanguinea emerged as the most prevalent species, exhibiting broad ecological adaptability. Non-native species, like Cheilomenes sexmaculata, were predominantly found in disturbed habitats, with some showing early signs of spreading into more natural environments, raising concerns about their potential impact on local biodiversity. These findings contribute valuable knowledge to understanding Coccinellidae diversity on San Cristóbal Island and highlight the importance of continued monitoring, particularly in the face of ongoing environmental change and the introduction of non-native species. This study underscores the need for targeted conservation efforts to protect the unique and fragile ecosystems of the Galápagos Archipelago.

Population Dynamics of Major Insect Pests and Natural Enemies in Tomato

An experiment on major insect pests and natural enemies of tomato was conducted during rabi, 2022-23 at the experimental farm for PG research, Biswanath College of Agriculture, Sonitpur. Results revealed eleven insect pests; whitefly, Bemisia tabaci (Gennadius); aphid, Aphis craccivora Koch; leafminer, Lyriomyza trifolii (Burgess); fruitborer, Helicoverpa armigera (Hubner); jassid, Amrasca biguttula biguttula (Ishida); semilooper, Trichoplusia ni (Hubner) were found to be the most dominant species. Two coccinellid predators viz., Micraspis discolor(F) and Cheilomenes sexmaculata (F) and two spiders Lycosa tista Tikader, Anyphaena accentuata (Walckenaer) were recorded as natural enemies. It was observed that the incidence of B. tabaci, A. craccivora and A. biguttula biguttula showed significant negative correlation with both maximum and minimum temperature. B. tabaci population registered a positive significant correlation with morning relative humidity. Both L. trifolii and H. armigera exhibited significant negative correlation with bright sunshine hour. Furthermore, T. ni showed significant negative correlation with maximum temperature and bright sunshine hour. As regards coccinellids, a significant negative correlation existed with maximum and minimum temperature, and rainfall and significant positive correlation with morning relative humidity. A significant positive correlation of aphids was also observed with coccinellids.

The Potential of Natural Enemies as Biological Control Agents for Various Pests on Water Spinach (Ipomoea aquatica F.)

Arthropods have the potential as biological control agents for various pests in kale plants. Some arthropod species have potential as natural enemies for biological control of plant pests due to their abundance and availability in the ecosystem. Some natural enemy arthropods consist of insects and spiders. Natural enemies found in the location of West Lombok District, NTB act as predators and parasitoids of pests on kale plants. The number of orders found were Araneae, Coleoptera, Hemiptera, Odonata and Hymenoptera. Four orders act as predators and one order, Hymenoptera as parasitoids. Natural enemy arthropods found that have the potential as natural enemies of pests in kale plants were found as many as 10 species, namely Argiope catenulate, Cicurina sp.., Oxyopes attenuates, Lepthypanthes tenuis, Cheilomenes sexmaculata, Coreus Marginatus, Leptoglossus occidentali sp., Onychogomphus forcipatus, Agriocnemis pieris, and Doryctobracon areolatus. While in Ogan Ilir District, South Sumatra, 1 species was found that acts as a natural enemy of the Hemiptera order, Gonocerus acuteangulatus.

Prey species restrict limb regenerative growth in a ladybird beetle, Cheilomenes sexmaculata: allocation costs of regeneration?

ABSTRACT In aphidophagous ladybird beetles, amputation of limbs in the larval stage leads to limb regeneration in adults. Regeneration is an energetically expensive phenomenon, and altering prey species may have profound consequences on this process. Therefore, we hypothesised that the quality of prey species will impact the ability to regenerate the lost limbs in Cheilomenes sexmaculata. For this experiment, we reared the larvae of C. sexmaculata on two aphid prey species: Aphis craccivora, which is the most preferred good-quality aphid, and A. nerii, which is the least preferred, poor-quality aphid. Although regeneration occurred in both dietary regimes, the extent of regeneration was higher in the beetles fed on A. craccivora. The length of the regenerated legs was shorter than the control/unamputated beetles, and it was further reduced when the prey species was of poor-quality indicating additional utilisation of resources for tissue repair and regeneration. Regeneration delayed pupal duration, post-amputation developmental duration and relative growth rate (RGR). Poor diet negatively affected developmental durations and RGR. Our results indicate prey dependency on regeneration and highlight energetic demands of regeneration and its associated developmental allocation costs.

DNA Isolation From Six Spotted Ladybird Beetle <i>Cheilomenes Sexmaculata</i> (F)

DNA extraction is a routine step in many insect molecular studies and extraction methods need to be evaluated for their efficiency, cost, and side effects, such as DNA degradation. Individuals of six spotted ladybird beetle, Cheilomenes sexmaculata (F.) were subjected to DNA extraction by the CTAB method and PureLink® Kit. The methods were compared in terms of DNA quantity and quality, cost of materials, and time consumed. The CTAB method resulted in higher DNA yield (ng DNA) at a much lower price and less degradation, as revealed on agarose gels. The PureLink® Kit was the most time-efficient but costliest, and degradation was observed on agarose gels. The DNA samples obtained were tested on agarose gel PCR for six SSRs located in various positions of the beetle’s genome. The results revealed that DNA isolated from the CTAB method showed successful amplifications, but the PureLink® Kit method did not show any amplification. These evaluations guide the choice of DNA extraction methods from C. sexmaculata beetles.

Foraging Behaviour of Sunflower Pollinators

A field experiment was carried out to gather data on the diversity and foraging activity of sunflower pollinators. The result revealed that distinct patterns among the various pollinators. Apis dorsata exhibited strong preference for evening foraging with highest visit rate observed at 18.00 hr, reaching an impressive visit of 28.96/ m2/ min followed by 12.00 hr (4.33/ m2/ min). Apis florea also displayed preference for evening foraging, with its peak visit rate of 0.40/ m2/ min occurring at 18.00 hrs. In case of Trigona iridipennis maximum visit noticed at 12.00 hr (1.43/ m2/ min) which was at par with visits at 14.00 hr (1.40/ m2/ min). Xylocopa fenestrata exhibited its peak foraging activity at 12.00 hr (0.10/ m2/ min). In case of lepidoptera, butterfly and skipper were visiting at morning session while moth preferred evening hours. More visits of Cheilomenes sexmaculata (0.14/ m2/ min) were recorded at 16.00 hrs. The diptera species, Ischiodon scutallaris exhibited higher activity level at 12.00 hr, with a visit rate of 0.08/ m2/ min.

Effect of Weather Parameters on Population Dynamics of Coccinellids in Sugarcane

Study of the population dynamics of coccinellids were carried out from 28th to 12th SMW during 2022-23. Two species Micraspis yasumatsui and Cheilomenes sexmaculata were found active throughout the whole study period. The population of Coccinellaseptempunctata, Propyleadissecta, Coccinella transversalis first appeared from 30thSMW and reach their peak population during 2ndSMW, whereas the population of Micraspis yasumatsuiand Cheilomenes sexmaculata started from 28th SMW and reach their peak during 52nd and 2ndSMW respectively. The coccinellids showed positive and highly significant correlation with relative humidity and negative and highly significance with maximum temperature.

The extent of regeneration is impacted by the stage of amputation in ladybird beetles: A case study in Cheilomenes sexmaculata

AbstractRegeneration has been a topic of interest across a range of taxa for centuries, and arthropods are no exception. Trade‐offs associated with regeneration are likely to involve the reallocation of resources away from other metabolic activities such as growth, development or reproduction. This might be reflected in costs to some developmental traits of the organism, despite regeneration being advantageous. These associated costs might also differ with the stage of injury or amputation. Here, we hypothesise that the extent of regeneration and trade‐offs associated with it may be stage‐specific. To test this hypothesis, the right forelimb of four larval stages of the ladybird beetle Cheilomenes sexmaculata (Fabricius) (Coleoptera: Coccinellidae) was amputated. Amputated individuals were reared until adulthood, and all developmental transitions were recorded. Regenerated legs in all the treatments were smaller than the controls, which did not experience the amputation, and the regenerative potency of early larval stages was higher than that of late larval stages. Limb regeneration caused delays in post‐amputation developmental duration in all the treatments, increasing their total developmental period. The length of the unamputated left foreleg as well as the wing and antenna size were also reduced in regenerated beetles, showing some internal trade‐off. However, there were no significant differences observed between regenerated and control adults in their fresh body weight and body size. Thus, limb regeneration depends upon the stage of larval development at which the amputation was performed. Amputation also affects the development of other appendages. The delay in normal beetle development might have been observed because of extra resource requirement, their allocation as well as reprogramming of the expression of some genes during regeneration.

Compatibility of Entomopathogenic Nematode (Steinernema sp.) and Coccinelid (Cheilomenes sexmaculata) with Insecticides registered against Fall Army Worm (Spodoptera frugiperda) in Corn

Cyantraniliprole, Chlorantraniliprole, Emamectin benzoate, Spinosad, Flubendiamide, Teflubenzuron, Novaluron+Indoxacarb, Broflanilide, Chlorafenapyr and Azadirachtin are some important registered insecticides against S. frugiperda in corn. Bioagents such as entomopathogenic nematodes (EPNs) and lady beetles have also role to reduce its population. Integration of bioagents with chemical insecticides is current need for sustaianable pest management. So, the present evaluation was conducted at laboratory condition to knowhow the fate of EPNs, Steinernema sp. and coccinelid (Cheilomenes sexmaculata) after their exposure with above mentioned insecticides used for S. frugiperda. Lab research was conducted during spring (2021) at BCKV, Burdwan campus (West Bengal). EPNs was multiplied on larva of Corcyra cephalonica. Cheilonemes sexmaculata (grubs and pupa) was collected from field. Ten novel insecticides recommended for S. frugiperda were evaluated against the mentioned bioagents. Corected mortality data of EPNs and coccinelid was analyzed statistically and accordingly insecticides were grouped into different toxicity class. Except Emamectin benzoate, all the chemicals were proved to be safe to Steinernema sp. E. benzoate caused 100 % reduction to insect infectivity by EPN, followed by Chlorafenapyr (80 %). Slightly harmful effect for grubs (C. sexmaculata) was noted in Cyantraniliprole and Emamectin benzoate and for pupa in emamectin benzoate and Novaluron + Indoxacarb. Otherwise all others were harmless both for grubs and pupal stage of C. sexmaculata. These pieces of information might be practical in decision making for IPM of S. frugiperda.

Chemotaxis response and age-stage, two-sex life table of the Cheilomenes sexmaculata (Fabricius) (Coccinellidae: Coleoptera) against different aphid species.

The Cheilomenes sexmaculata (Fabricius) (Coleoptera: Coccinellidae), is one of the most beneficial and identifiable predators of numerous soft-bodied and sucking insect pests of several crops. Biological parameters and olfactory response of C. sexmaculata were investigated under laboratory conditions by providing three different aphid species i.e., mustard aphid (Lipaphis erysimi Kaltenbach), citrus black aphid (Toxoptera citricida Kirkaldy), and peach aphid (Diuraphis noxia Kurdjumov) as a food source. The developmental period of immature stages of C. sexmaculata was shorter on D. noxia as compared to other aphid species. The adult longevities were longer on D. noxia and T. citricida while shorter on L. erysimi. Female fecundity was highest on D. noxia while lowest on L. erysimi. Life table parameters i.e., intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (Ro), and gross reproductive rate (GRR) were maximum on D. noxia while minimum on L. erysimi. The mean generation time C. sexmaculata was 20.90, 23.69, and 26.2 days on D. noxia, L. erysimi, T. and citricida, respectively. These findings were further confirmed from the olfactory experiment where D. noxia proved to be the most preferred prey. This study provides necessary information for mass-rearing of C. sexmaculata.

Population Dynamics of Mustard Aphid <i>Lipaphis erysimi</i> (Kalt) and its Coccinellid Predators

Field experiments were conducted during 2021-2022 and 2022-2023 to determine the population dynamics of mustard aphid Lipaphis erysimi (Kalt) and its coccinellid predators on two mustard varieties viz. Rohini (highly susceptible) and RH0749 (least susceptible) in relation with different abiotic factors. The infestation was observed in last week of December to the first week of March, the overall mean indicates the incidence was at its peak in the second week of February. Maximum incidence of 160-120/ five plants was recorded on Rohini while 160-100/five plants on RH0749. The predatory activities of coccinellids viz., Cheilomenes sexmaculata (L), Coccinella septempunctata (F) and Coccinella transversalis (F) revealed significant positive correlation with aphid incidence.

Heterospecific interference modulates the reproductive traits of a ladybird beetle

AbstractReproductive interference between species is vital to understand interspecific interactions along with their ecological and evolutionary patterns in nature. We studied potential reproductive interference between two sympatric species of ladybird beetles Propylea dissecta and Cheilomenes sexmaculata (Coleoptera: Coccinellidae). Our aim was to determine whether heterospecific matings occur between these two species, and if so, how they affect the reproductive output of females of both species. We found that heterospecific mating occurs, with P. dissecta females mating with C. sexmaculata males, but not vice versa. To compare the effect of conspecific and heterospecific males on the reproductive output of P. dissecta females, we used two mixed mating treatments: conspecific mixed (an additional P. dissecta male with conspecific pair of P. dissecta), and heterospecific mixed (a C. sexmaculata male with conspecific pair of P. dissecta). We found that conspecific interference did not affect reproductive parameters. However, the presence of a heterospecific C. sexmaculata male interfered with conspecific matings in P. dissecta through multiple mating attempts, resulting in reduced fitness and reproductive success of P. dissecta. Our results indicate that the presence of heterospecific males significantly affected the reproductive output of P. dissecta, apparently as a result of the greater attempted mating activity of C. sexmaculata males. Our findings may be relevant for effective biocontrol strategies involving these insect species.

TRITROPHIC ASSOCIATION AND DISTRIBUTION OF APHIDOPHAGOUS COCCINELLID CHEILOMENES SPP. (COCCINELLIDAE: COLEOPTERA: INSECTA) IN INDIA

The paper deals with the prey records of Cheilomenes spp. in different states of India. In India, only two species of the genus Cheilomenes Chevrolat, 1837 - Cheilomenes propinqua (Mulsant, 1850) and Cheilomenes sexmaculata (Fabricius, 1781) are known to prey aphids. Cheilomenes propinqua is recorded in India on only one aphid species Aphis (Aphis) gossypii Glover, 1877 infesting brinjal in Assam and Meghalaya. The Cheilomenes sexmaculata (Fabricius, 1781), also known as six-spotted zigzag ladybird, preys on 76 species of aphids infesting 160 species of plants belonging to several families distributed throughout India except Jharkhand and Mizoram states. In union territories, it is recorded preying on aphids only in Jammu and Kashmir and Delhi. The most preferred prey is Aphis (Aphis) gossypii Glover which was found infesting 53 plant species distributed in 21 states/union territories followed by Aphis (Aphis) craccivora Koch infesting 35 plant species distributed in 24 states/union territories and Myzus (Nectarosiphon) persicae (Sulzer) infesting on 20 plant species distributed in 16 states/union territories. Total number of tritrophic relationship of Cheilomenes sexmaculata (Fabricius) (predator-prey-host plant) reported in India was 282; maximally being in Gujarat (72) followed by Uttar Pradesh (56), West Bengal (46), Bihar (43), Karnataka (41), Manipur (38), Maharashtra (25), and less than it in other states.

Population dynamics of insect pests and their natural enemies in pearl millet ecosystem

Field experiment was carried out at the Regional Agricultural Research Station, Vijayapura, to study population dynamics of insect pests and their natural enemies in pearl millet ecosystem. The present study revealed that, dead heart per cent (14.45%) due to shoot fly, Atherigona approximata (Malloch) was peak during 35th Standard Meteorological Week (SMW), per cent damage due to stem borer, Chilo partellus (Swinhoe) was at peak during the 37th SMW (4.55%) and highest blister beetle, Lytta sp. population was observed during 38th SMW (12 beetles/ 5 Ear heads). While, the maximum population of aphid Rhopalosiphum maidis (Fitch) and its predators, such as coccinellids Cheilomenes sexmaculata (Fabricius), Harmonia octomaculata (Fabricius) and spiders were recorded during the 34th SMW and the green lacewing Chrysoperla zastrowi sillemi (Ebson-petersen) population at 35th SMW. The correlation studies indicated that, dead heart per cent due to shoot fly revealed a significant positive correlation with maximum temperature (r= 0.588*) and stem borer infestation revealed a significant positive correlation with maximum relative humidity (r= 603*) and sunshine hours (r= 585*). None of the weather parameters showed a significant correlation with the incidence of blister beetle and aphid populations.

Intra- and inter-specific interactions between <i>Coccinella transversalis</i> and <i>Cheilomenes sexmaculata</i> in relation to prey abundance

In the present study, we investigated the feeding rate and the effect of intra- and inter-specific interactions between adults, 3rd and 4th instar larvae of the coccinellids, Coccinella transversalis and Cheilomenes sexmaculata to its conspecific and heterospecific combinations under laboratory conditions. Cannibalism and the effect of interaction were investigated using the multiplicative risk model. Aphidophagous predators and their instars were kept alone, paired with conspecifics, and heterospecifics combinations in the absence and presence of prey (aphid, Aphis gossypii). In the presence of aphids, the combined release of these predator species revealed that except for (3rd × 3rd) and (4th× 4th) intraspecific combination of both predators, the observed consumption was lower than the expected consumption. In the case of interspecific combination, the observed consumption was significantly lower than expected and it indicated the antagonistic effect of both the predators in later instars in the presence of sufficient prey population. In the absence of the prey, intra-specific cannibalism was lowest between adults of both the species and highest among C. transversalis 3rd × C. transversalis adult and C. sexmaculata 3rd × C. sexmaculata adult. The interspecific predation was highest between C. transversalis adult × C. sexmaculata 3rd instar and lowest between adults of both species. The results suggest that C. transversalis was more a successful aphid predator, being able to find aphids more quickly and consume more of them compared to C. sexmaculata. As there was less intraspecific cannibalism among C. transversalis and individual consumption is also high, it is considered to play a significant role as a biocontrol agent in aphid management.

Seasonal Incidence of Different Insect Pests and Their Natural Enemies in Tomato Ecosystem

A field investigation was carried out to analyze the seasonal incidence of different insect pests and their natural enemies in tomato ecosystem along with their correlation with weather parameters from December, 2020 to April, 2021. A total number of 10 insect pests and 11 natural enemies were documented throughout the season. Among those insect pests recorded, tomato fruit borer, Helicoverpa armigera was the major one (49.91% relative abundance) followed by aphid, Aphis gossypii (18.62%), serpentine leaf miner, Liriomyza trifolii (16.20%) and whitefly, Bemisia tabaci (7.33%). Among the natural enemies, coccinellids were found abundantly and Coccinella transversalis recorded the highest relative abundance of 26.77% followed by Cheilomenes propinqua (10.23%), Cheilomenes sexmaculata (10.23%) and Coccinella septempunctata (9.44%). The findings of the experiment indicated that the maximum population of Helicoverpa armigera and L. trifolii was observed on the 10th SMW. While in case of A. gossypii it was observed on the 7th SMW respectively. The correlation between insect infestations and meteorological conditions demonstrated that the min temp. showed notable positive correlation with H. armigera and Aphis gossypii population and considerable negative correlation with L. trifolii. The max temp. showed noteworthy negative correlation with L. trifolii and Aphis gossypii. The morning relative humidity was found to possess significant positive correlation with L. trifolii and negative correlation with H. armigera. The evening relative humidity showed negative correlation with L. trifolii and Aphis gossypii. The study's findings provide a clear picture of the level of harm caused by insect pests in addition to helping us anticipate the seasonal occurrence of these pests for the purposes of pest monitoring and management.

Interaction of Myzus spp. (Hemiptera: Aphididae) with their Food Plants, Parasitoids and Predators in Northeast Bihar

The article examines the association of aphids along with their food plants, parasitoids and predators in varying ecological conditions for the possible use in biological control programs. Aphids are small, sap-sucking insects and they are considered an important pest of agricultural and horticultural plants due to sucking of vital nutrients and transmitting viral diseases. During the survey, 41 species of aphids were recorded on 122 plant species in northeast Bihar. Three species of Myzus viz., M. ascalonicus, M. persicae and M. variants were recorded. M. persicae was recorded on 36 food plants from December to April but its peak population was observed during February and March. Five species of parasitoids (Aphelinus albipodus, Aphelinus gossypii, Aphidius colemani, Binodoxys indicus, Diaeretiella rapae, Lipolexis oregmae), five species of coccinellid predators (Cheilomenes sexmaculata, Coccinella septempunctata, Coccinella transversalis, Micraspis discolor, Scymnus pyrocheilus) and three species of syrphid predators (Dideopsis aegrota, Ischiodon scutellaris, Melanostoma orientale) were recorded on M. persicae. Among these, three parasitoids (B. indicus, L. oregmae, A. gossypii), four coccinellids predators (C. sexmaculata, C. septempunctata, C. transversalis, S. pyrocheilus) and two syrphid predators (D. aegrota, I. scutellaris) were observed abundantly in all localities with a high rate of parasitization/predation on M. persicae.

Effect of larval thermal conditions on limb regeneration in a ladybird beetle, Cheilomenes sexmaculata.

In view of global environmental change, ecological factors especially temperature, affect development of the poikilotherms like insects. Since ladybirds are at risk of injury under mass-rearing conditions, their ability to regenerate injured limbs is highly crucial for their survival. Therefore, the effect of limb regeneration in relation to temperature forms the basis of the present study. The immature stages of insects, being more vulnerable to the surrounding temperature, were considered to study the effect of the prior thermal experience of larvae on regeneration. We exposed the early larval stages of the ladybird beetle, Cheilomenes sexmaculata, to different temperature conditions pre- and postamputation. Exposure of immature stages to extreme temperatures did not affect the ability to regenerate and regeneration occurred at given temperature conditions. However, the regenerated legs were smaller in size across given temperatures as compared to unamputated legs. Body weights in amputated treatments showed no difference and remained unchanged across temperatures when compared to unamputated treatments. Postamputation developmental duration, equivalent to recovery time postlimb amputation, was found to be affected by larval thermal conditions. Recovery was faster in larval treatments exposed to higher temperatures. Thus, larval thermal conditions though did not affect the ability to regenerate lost limbs directly, it does modulate the time taken to regenerate.