Chebula Research Articles

Investigation of the effects of Terminalia chebula seed hydroalcoholic extract on Paraoxonase1 enzyme activity in rats

Background and aims: Paraoxonase 1 (PON1) is related to high-density lipoprotein (HDL) in serum and protects against low-density lipoprotein (LDL) oxidation. This study aimed to investigate the effects of Terminalia chebula on PON1 in hyperlipidemic rats and the molecular docking effects of some compounds of this medical plant on PON1 activity. Methods: Overall, 40 male rats (200–250 gr) were randomly divided into four groups, including the control group, the hyperlipid group, the hyperlipid group receiving 400 mg/kg of the hydroalcoholic extract of T. chebula seeds, and the hyperlipid group receiving 800 mg/kg of the hydroalcoholic extract of T. chebula seeds. The PON1 arylesterase activity in serum and the PON1 gene expression in the liver tissue underwent investigation. Then, the molecular docking effects of its compounds were studied on PON1 through in-silico studies using the AutoDock software (version 4.2.0). Results: T. chebula decreased (P<0.001) the serum triglycerides from 105.88±10.15 mg/dL in the hyperlipidemic group to 66.88±14.90 and 74.25±9.51 mg/dL in hyperlipidemic groups receiving 400 and 800 mg/kg of the hydroalcoholic extract of T. chebula seeds, respectively. In addition, the PON1 serum aryl esterase activity increased from 202.12±6058 in hyperlipidemic rats to 224.34±58.74 (P=0.83) and 235.80±37.05 (P=0.6) in hyperlipidemic groups receiving 400 mg/kg and 800 mg/kg of the hydroalcoholic extract of T. chebula seeds, respectively. It also demonstrated a significant effect on PON1 gene expression (P<0.001). In addition, the in-silico and docking results revealed that the main antioxidant compounds of T. chebula, such as catechin, kaempferol, and quercetin, could bind to the PON1 enzyme directly and influence the enzyme activity. Conclusion: T. chebula increased PON1 activity and PON1 gene expression. However, among the plant’s compounds, catechin, kaempferol, and quercetin played the most substantial role in the PON1 activity. It seems that these compounds can be useful as co-treatments in hyperlipidemia therapies.

Augmenting the antioxidant, anti-bacterial and anti-carcinogenic potential of Terminalia chebula and Terminalia bellirica after tannin acyl hydrolase mediated biotransformation

Tannins, the fourth most abundant plant compounds, comprise condensed and hydrolysable types. Proanthocyanidins are flavan-3-ol polymers, while hydrolysable tannins, categorized as gallotannins and ellagitannins, form esters of gallic/ellagic acid and poly-ol. Tannin acyl hydrolase catalyzes hydrolysable tannin breakdown, yielding gallic acid derivatives with antibacterial, anti-hyperglycemic, anti-inflammatory, antioxidant, anti-carcinogenic properties. In this study, we explored hydrolysable tannins in extracts from Terminalia chebula and Terminalia bellirica, focusing on their phenolic and tannin content, as well as enzymatic and biological activities. Quantitative assessments for Total Phenol Content (TPC), Tannic Acid Content (TAC), and Hydrolysable Tannin Content (HTC) were carried out with Terminalia chebula displaying maximum amount of all three i.e. 291.9mg GAE/g DW TPC, 266.7 mgTAE/g DW TAC and 246.6 mg TAE/g DW HTC. LCMS-MS analysis revealed 8 and 13 gallic acid derivatives from T. bellirica and T. chebula, respectively after enzymatic hydrolysis. Following enzymatic treatment, the IC50 values for T. bellirica were slightly higher than T. chebula. The tannase hydrolysed T. chebula demonstrated the highest zone of inhibition against E. coli whereas the un-hydrolysed T. chebula showed minimal inhibition across all four bacterial cultures under study. The hydrolysed extract of T. chebula exhibited significant cytotoxicity after a 20-min hydrolysis, demonstrating a substantial effect with a minimal IC50 value of 83.56 μg/ml against HCT-116 cell line. Our study quantitatively highlights the enhanced bioactive properties conferred by enzymatic hydrolysis of hydrolysable tannins and considerable health benefits associated with these compounds.

Terminalia Chebula Fruits Powder as Tribopositive Material for High‐Performance Triboelectric Nanogenerator

Triboelectric nanogenerator (TENG) has generated a lot of attention for developing self‐powered/battery‐free technology. These kinds of energy harvesting devices are required for a sustainable future. In this work, the TENG is developed by using the Terminalia Chebula (T. Chebula) biomaterial as tribopositive material. The physiochemical characterizations suggest that T. Chebula has hydroxyl and carbonyl groups which are important to get tribopositivity from the material. The T. Chebula tribopositive material has been tested for the TENG with different electronegative layers such as polyethylene terephthalate (PET), polytetrafluoroethylene (PTFE), and polydimethylsiloxane (PDMS). The T. Chebula/PTFE device exhibited a higher output performance. This pair was used for the area and frequency‐dependent studies. In the present case, 16 cm2 device area and 5 Hz frequency conditions show outstanding device performance. Furthermore, the device has been employed for operating low‐power electronic devices such as LEDs and a calculator which operated without any degradation. These results suggested that T. Chebula is a potential tribopositive material for sustainable energy harvesting technology.

Encapsulation of bioactive compounds extracted from haritaki pulp (Terminalia chebula Retzius): characterization of physical, thermal, and morphological properties

The bioactive compounds of haritaki (Terminalia chebula Retzius) were microencapsulated using zein and starch as the encapsulating agents, utilizing both conventional (encapsulator) and advanced (freeze drying) techniques.

A randomized, double-blind clinical trial of poly-herbal traditional decoction for the management of type 2 diabetes in comparison with standard Western treatments

Abstract Introduction: Type 2 diabetes mellitus (T2DM) known as Mathumegam is a significant health concern in the twenty-first century. Despite great advances in contemporary science, there is a lack of moderately safe and effective drugs for treatment. The purpose of this study was to evaluate the efficacy of a poly herbal traditional decoction containing 10 herbs such as Senna auriculata (root & bark), Cissampelos pareira (root), Ficus racemosa (bark), Terminalia arjuna (bark), Acacia arabica (bark), Syzygium cumini (bark), Curcuma longa (rhizome), Terminalia chebula (fruit pulp), Phyllanthus emblica (dry fruit), and Terminalia bellirica (fruit pulp) in the management of T2DM. Materials and Methods: It was a double-blind, randomized controlled trial involving 100 T2DM patients who were not well managed by diet or exercise. For a period of 12 weeks, 50 patients in group A were administered 5g of the test medication mixed with 100ml (one tumbler) of hot water for 10 to 15 min, as well as standard Western therapy. Group B patients (n=50) received a placebo in addition to normal Western therapy. The main focus of the study was to achieve a 3-month decrease in glycosylated hemoglobin (HbA1c) levels from the starting point, with additional objectives including lowering fasting blood glucose (FBG) levels and observing changes in diabetic symptoms. Results: At the end of the trial, mean fasting blood glucose levels decreased to 119.04 ± 11.52 and 176.90 ± 13.91 mg/dL in the test and control groups, respectively. Group A showed a more significant decrease in mean fasting blood glucose levels compared to group B. This indicates that the daily use of poly herbal traditional decoction along with standard allopathic medical therapy for 3 months led to notable reductions in FBG levels. Similarly, group A showed greater improvement in the measured signs and symptoms of diabetes, such as polydipsia, polyuria, polyphagia, weariness, and burning sensation in the hands and feet compared to group B throughout the trial. At baseline, the mean HbA1c (%) in group A and group B was 7.47 ± 0.70 and 7.39 ± 0.66, respectively. After 12 weeks of investigation, the levels of glycated hemoglobin were lowered to 5.03 ± 0.53 and 6.79 ± 0.51 respectively. There were no adverse events reported during the trial period. Conclusion: This study found that the experimental medication was beneficial in T2DM, radically lowering FBG and HbA1c levels in diabetic individuals after 12 weeks of treatment.

Effect of laxative polyherbal paste for loperamide induced constipation in rats.

The goal of this study was to find out if polyherbal paste (PHP) with Rosadamascena, Terminalia chebula, and Trachyspermumammi in honey could help rats that were constipated because of loperamide. Thirty male rats were divided into 6 groups: a control group receiving saline, a model group receiving loperamide at 10 mg/kg and saline, a phenolphthalein group (positive control) receiving loperamide at 10 mg/kg and phenolphthalein at 10 mg/kg, and low (20 mg/kg), medium (40 mg/kg), and high (60 mg/kg) doses of PHP, via intragastric administration for 7 days. Various parameters, including food consumption, water consumption, body weight, fecal characteristics, gastrointestinal transit rate, histological changes, serum biomarkers, and aquaporin-3 (AQP3) and C-kit protein expression levels, were assessed. Administering PHP at a dose of 60 mg/kg resulted in a 16.89% increase in fecal water content, a 12.14% increase in the amount of feces, and a 23.67% increase in gastrointestinal transit rate, while also reducing the time to black stool and restoring appearance by 23.41%. At the 40 mg/kg dose, PHP increased motilin levels in the blood by 31.22%, gastrin by 52.78%, and substance P by 19.45% while decreasing somatostatin by 20.17%. Furthermore, at the 60 mg/kg dose, PHP decreased mucous membrane damage and goblet cell function in the colon, reduced AQP3 protein production by 33.39%, and increased c-kit protein production by 12.14%. The PHP showed promising therapeutic potential for loperamide-induced constipation in rats.

An integrative approach with TROPIS, Tube in Tract and IFTAK therapy in the management of Complex Anal Fistula: An experience

The management of complex anal fistulae is a challenging job because of the possible risk of damage to the anal sphincter leading to fecal continence, high reoccurrence rate, and delayed wound healing. Other challenges include proper drainage of the tract without disturbing anatomical alignment and painless defecation. This case presented to the outpatient department with a history of recurrent boil in the perianal region and painful pus discharge from the site for 15 days. The case was diagnosed as a case of complex fistula-in-ano and was managed by an integrative approach operative procedure under spinal saddle block anesthesia. Principles of application of Interception of Fistulous Tract and Application of Ksharasutra (IFTAK), transanal opening of intersphincteric space, and tube in tract technique were applied in the management. With necessary analgesia and antibiotics for five days, the patient was given Triphala guggulu internally, and sitz bath with Haritaki (Terminalia chebula Retz.) and Neem (Azadirachta indica A. Juss.) decoction, postoperatively. Wound dressing was done by application of a gauze piece soaked in Apamarga kshara taila. The management resulted in a painless recovery and the patient started attending his daily routine activities from the 7th post-operative day onwards. Pus discharge ceased soon after the operative procedure. Complete healing of postoperative wounds was achieved in ten weeks with prescribed postoperative care and regimen. For the last year, the patient is free from anal discomfort and no reoccurrence has been reported till now.

Safety Evaluation of Vatari Guggulu through Acute and 90-day Repeated Dose Toxicity Study in Experimental Animals

Abstract Background: Vatari Guggulu is an Ayurvedic Medicine used in the treatment of Vata disorders. It contains Vatari taila (Castor oil), Shuddha gandhaka (purified Sulphur), Haritaki (Terminalia chebula Retz.), Vibhitaki (Terminalia bellirica [Gaertn.] Roxb.), Amla (Emblica officinalis Gaertn) and guggul (Purified Commiphora mukul Hook). As per the Ayurvedic texts, it is widely used clinically in the treatment of rheumatoid arthritis, hip pain, sciatica, gout, joint pain, and burning sensation. However, there are no systematic toxicity studies were performed to scientifically evaluate the safety of Vatari Guggulu. Objectives: The present study was designed to investigate the safety of Vatari Guggulu in acute and 90-days repeated dose oral toxicity as per Organization for Economic Cooperation and Development guidelines 423 and 408. Materials and Methods: During the acute toxicity, Vatari Guggulu was orally administered at a single limit dose of 2000 mg/kg. The mortality and clinical signs were observed for 14 days. Similarly, the 90-days repeated dose toxicity was studied by administering the Vatari Guggulu at a limit dose of 1000 mg/kg. Results: In the acute toxicity, there were no signs of toxicity, and the LD50 was calculated as >2000 mg/kg. During the 90 days of repeated dose toxicity, the Vatari Guggulu at the dose of 1000 mg/kg did not show any signs of toxicity as compared to the control. The hematological, serum biochemical, and urine analysis profile of the Vatari Guggulu at the dose of 1000 mg/kg did not show any significant difference as compared to the control (P > 0.05). Further, the histopathological data of control and Vatari Guggulu-treated rats were found to be normal. Conclusions: It was concluded that the test drug Vatari Guggulu is found to be safe upto 2000 mg/kg in acute toxicity and 1000 mg/kg in 90-days repeated dose oral toxicity study. The NOAEL of Vatari Guggulu is established as 1000 mg/kg/day.

Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells

Background: Recently, many approaches have been conducted in natural skin healthcare, with herbal remedies gaining prominence due to their perceived efficacy and fewer side effects. Herbal soaps, in particular, are noted for their antimicrobial, anti-inflammatory, and antioxidant properties, making them appealing for acne-causing bacteria such as Propionibacterium acnes, Terminalia chebula, a medicinal plant with a rich history of traditional medicinal properties, including antibacterial effects. However, limited information exists on its activity against acne-causing bacteria and its cytotoxicity on human skin cells. Methods: Our study demonstrated the anti-propionibacterium activity using a novel Terminalia chebula herbal soap formulation (TC-HSF), and to determine its cytotoxicity on Human skin fibroblast cells, Terminalia chebula aqueous extract was used to make herbal soap, the antibacterial activity was performed against wild isolated propionibacterium acne strain using well-diffusion method, MIC and time-kill Kinetics, several concentrations were evaluated for cytotoxicity on Human skin fibroblast cell lines. Results Our findings indicate a potent activity of TC-HSF against P.acne at 0.18mg\ml with lower CFU\ml in tested intervals and high cell viability 98%, IC50 Value was 50mg\ml. Conclusion: Our study highlights the promising antibacterial efficacy of TC-HSF against Propionibacterium acnes and its cytotoxic effects on human skin fibroblast cells. The research adds valuable insights into the potential of TC-HSF for acne management and underscores the importance of dosage considerations in the formulation of herbal soap products.

The Terminalia chebula retz extract treats hyperuricemic nephropathy by inhibiting TLR4/myd88/NF-κb axis

Ethnopharmacological relevanceHyperuricemic nephropathy (HN) is a renal injury caused by hyperuricemia and is the main cause of chronic kidney disease and end-stage renal disease. ShiWeiHeZiSan, which is composed mainly of components of Terminalia chebula Retz. And is recorded in the Four Medical Tantras, is a typical traditional Tibetan medicinal formula for renal diseases. Although T. chebula has been reported to improve renal dysfunction and reduce renal cell apoptosis, the specific mechanism of the nephroprotective effects of T. chebula on HN is still unclear. Aim of the studyThis study was conducted to evaluate the effects and specific mechanism of T. chebula extract on HN through network pharmacology and in vivo and in vitro experiments. Materials and methodsPotassium oxalate (1.5 g/kg) and adenine (50 mg/kg) were combined for oral administration to establish the HN rat model, and the effects of T. chebula extract on rats in the HN model were evaluated by renal function indices and histopathological examinations. UPLC-Q-Exactive Orbitrap/MS analysis was also conducted to investigate the chemical components of T. chebula extract, and the potential therapeutic targets of T. chebula in HN were predicted by network pharmacology analysis. Moreover, the activation of potential pathways and the expression of related mRNAs and proteins were further observed in HN model rats and uric acid-treated HK-2 cells. ResultsT. chebula treatment significantly decreased the serum uric acid (SUA), blood urea nitrogen (BUN) and serum creatinine (SCr) levels in HN rats and ameliorated renal pathological injury and fibrosis. A total of 25 chemical components in T. chebula extract were identified by UPLC-Q-Exactive Orbitrap/MS analysis, and network pharmacology analysis indicated that the NF-κB pathway was the potential pathway associated with the therapeutic effects of T. chebula extract on HN. RT‒PCR analysis, immunofluorescence staining and ELISA demonstrated that the mRNA and protein levels of TLR4 and MyD88 were significantly decreased in the renal tissue of HN rats after treatment with T. chebula extract at different concentrations, while the phosphorylation of P65 and the secretion of TNF-α and IL-6 were significantly inhibited. The results of in vitro experiments showed that T. chebula extract significantly decreased the protein levels of TLR4, MyD88, p-IκBα and p-P65 in uric acid-treated HK-2 cells and inhibited the nuclear translocation of p65 in these cells. In addition, the expression of inflammatory factors (IL-1β, IL-6 and TNF-α) and fibrotic genes (α-SMA and fibronectin) was significantly downregulated by T. chebula extract treatment, while E-cadherin expression was significantly upregulated. ConclusionT. chebula extract exerts nephroprotective effects on HN, such as anti-inflammatory effects and fibrosis improvement, by regulating the TLR4/MyD88/NF-κB axis, which supports the general use of T. chebula in the management of HN and other chronic kidney diseases.

A Comparative Analysis for Phytochemical Screening and Antioxidant Potential of Two Different Formulations of <i>Triphala</i> Extract

In Ayurveda, Triphala is a formulation of three herbs. It is a mixture (equiproportional 1:1:1) of fruits of three plants, Haritaki (Terminalia chebula), Bibhitaki (Terminalia bellerica) and Amalaki (Emblica officinalis). Triphala shows antibacterial, antiallergic, antiviral, antifungal, immunomodulatory and antioxidant properties. Triphala and its constituents help in the improvement of blood circulation, reduction in level of cholesterol and control of blood pressure. This study focuses on detecting various phytocompounds and their comparative analysis for antioxidant potential in two different formulations of Triphala extract. Antioxidant activity was determined by phosphomolybdenum assay and reducing power assay. Our results indicated the presence of various phytocompounds such as phenols, flavonoids, tannins, saponins, alkaloids etc, which may play a role in its biological activities. Both Triphala formulations showed significant free radical-scavenging properties as compared to ascorbic acid. Formulation S2 (H:B:A - 1:2:3) showed higher antioxidant activity as compared to formulation S1 (H:B:A- 1:1:1). In conclusion, Triphala non-equiproportional mixture may be a more promising Ayurvedic drug in future Triphala.

Ethyl acetate extract of Terminalia chebula alleviates DSS-induced ulcerative colitis in C57BL/6 mice.

Background: The Chinese pharmacopeia records Terminalia chebula as effective in treating prolonged diarrhea and dysentery, blood in the stool, and prolapse. Modern pharmacological research proves it has multiple pharmacological benefits, including antioxidant, anti-inflammatory, analgesic, hepatoprotective, neuroprotective, and other properties. Objectives: This study aims to clarify the role of Terminalia chebula's ethyl acetate extract (TCEA) on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice, as well as explore the potential mechanism of action. Materials and methods: The variation of different extracts of T. chebula was detected using the HPLC technique, and the main components in TCEA were identified. DSS was used to establish a mouse model to mimic the physiological state of UC in humans; the alleviating effect of TCEA and positive control 5-ASA on UC mice were evaluated by gavage treatment. Disease progression was assessed by monitoring the mouse's weight change and disease activity index (DAI). The changes in colon tissue were estimated by measuring colon length, HE, and AB-PAS staining and detecting oxidative stress parameters. The results draw from Western blot and real-time PCR showed the TLR4/MyD88/NF-κB pathway may involve in the anti-inflammatory activity of TCEA. Furthermore, the gut flora sequencing technique was employed to monitor the differentiation of intestinal microbiota of mice induced by DSS and TCEA treatment. Results: TCEA significantly lowered DAI scores and inhibited the weight loss and colonic shortening induced by DSS. The colon histomorphology and oxidative stress levels were enhanced after TCEA treatment compared with DSS induced UC group. TCEA attenuated the inflammatory response by regulating TLR4/MyD88/NF-κB pathway activation. Intestinal flora sequencing showed that DSS and TCEA greatly impacted mice's composition and diversity of intestinal microorganisms. But TCEA increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes and Proteobacteria compared with the DSS group, which contributed a lot to returning the intestinal flora to a balanced state. Conclusion: This study confirms the alleviating effect of TCEA on UC and provides new ideas for developing TCEA into a new drug to treat UC.

Baiheqingjin formula reduces inflammation in mice with asthma by inhibiting the PI3K/AKT/NF-κb signaling pathway

Ethnopharmacological relevanceBaiheqingjin Decoction (BHQJ), which consists of 7 traditional Chinese herbs including Baibu (Stemona tuberosa Lour.), Hezi (Terminalia chebula Retz.), Mahuang (Ephedra sinica Stapf.), Ziwan (Aster tataricus L. f.), Dilong (Pheretima), Sangbaipi (Morus alba L.), and Xianhecao (Agrimonia pilosa Ledeb.). BHQJ is commonly used for treating cough asthma, and variant cough-variant asthma as it, is effective in improving asthma symptoms and reducing airway inflammation. Aim of the studyTo investigate the mechanisms of BHQJ in treating allergic asthma. Materials and methodsWe collected information about the components and targets of 6 Chinese medicines (excluding Pheretima) from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Additionally, we obtained genes associated with asthma from six disease databases. To create a protein-protein interaction network, we conducted an intersection analysis using differentially expressed genes derived from RNA transcriptome data. Subsequently, we carried out Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses. To validate the findings from network pharmacology and transcriptomics, we established an allergic asthma mouse model induced by ovalbumin and conducted in vivo experiments. ResultsUsing network pharmacology and transcriptomics analyses, we identified the pathways including the PI3K/AKT signaling pathway, and NF-κB signaling pathway. Among these, the involvement of the PI3K/AKT/NF-κB signaling pathway in various pathological processes of asthma, such as airway inflammation, smooth muscle contraction, and excessive mucus production, are well-documented. Histopathological examinations indicated that BHQJ had the potential to mitigate inflammatory cell infiltration and the excessive growth of goblet cells in the airways of asthmatic mice, consequently reducing mucus secretion. Results from Western blot demonstrated that BHQJ could inhibit the activation of the PI3K/AKT/NF-κB pathway at the protein levels. Enzyme-linked immunosorbent assay findings revealed that BHQJ could reduce the production of typical “type 2 asthma” cytokines and immunoglobulin (Ig) E in the blood. These discoveries imply that BHQJ has the potential to reduce the release of inflammatory cytokines and suppress the overactivation of the PI3K/AKT/NF-κB signaling pathway, thus offering a therapeutic approach for asthma. ConclusionOur research offers initial insights into the fundamental mechanisms through which BHQJ treats asthma. This study reveals the potential mechanism of BHQJ in treating asthma, particularly its role in reducing inflammatory cytokines, mucus production, and cell infiltration, as well as inhibiting the expression of PI3K/AKT/P65 phosphorylated protein. These findings indicate the potential of BHQJ in treating asthma. In summary, our study provides preliminary insights into the asthma treatment mechanism of BHQJ and provides guidance for future research.

Green Synthesis of Copper Oxide Nanoparticles Synthesized by Terminalia chebula Dried Fruit Extract: Characterization and Antibacterial Action.

Copper oxide nanoparticles (CuONPs) have emerged as potential antibacterial agents. In this study, we aimed to synthesize CuONPs using Terminalia chebula(T. chebula)dried fruit extract and evaluate their antibacterial activity against specific wound pathogens. Our primary objective was to comprehensively characterize driedT. chebula fruit (TCF)-CuONPs and assess their antibacterial efficacy. CuONPs were synthesized through a green synthesis approach employing T. chebula dried fruit extract. Structural and compositional characterization involved UV-visible spectroscopy, scanning electron microscopy (SEM), elemental dispersive X-ray analysis (EDX), and transmission electron microscopy (TEM). The antibacterial activity of CuONPs was assessed against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli through various assays, including agar well diffusion, time-kill curve, protein leakage analysis, and antibiofilm assays. Characterization revealed a distinct absorption peak at 440 nm in UV-visible spectroscopy, spherical morphology under SEM, and the presence of copper in EDX analysis. TEM revealed nanoparticle dimensions of approximately 10-12 nm. In antibacterial assays, TCF-CuONPs displayed significant efficacy, with Pseudomonas aeruginosa exhibiting heightened susceptibility. This study successfully synthesized eco-friendly copper oxide nanoparticles using T. chebuladried fruit extract and thoroughly characterized their structural and compositional attributes. CuONPs exhibited substantial antibacterial potency against specific wound pathogens, indicating their potential in wound management applications. These findings contribute to the development of sustainable antibacterial solutions with implications for healthcare and environmental sustainability. Further research can delve into the mechanisms and broader applications of CuONPs based on the specific experimental outcomes.

Quantitative comparison on the similarities and differences of Mongolian medicines in the treatment of liver diseases between China and Mongolia based on data mining

Abstract Objective The R language was used to quantitatively compare the medication patterns of Mongolian medicines used for the treatment of liver diseases between China and Mongolia, with a view to provide a reference for the research and clinical application of Mongolian medicines in the treatment of liver diseases. Methods The Mongolian medicinal prescriptions used for treating liver disease in Mongolia were collected from the Mongolian Pharmacy and Mongolian Treatment Guide for Common diseases in Mongolian Medicine, and those used in China were collected from the Inner Mongolia Standard for Mongolian Proprietary Medicine and Annotation of Mongolian Medicine Preparation Specifications. After the database was established, the frequency, properties, and tastes of the Mongolian medicines were analyzed, and R studio was used for the association rules analysis and cluster analysis. Results A total of 27 prescriptions used in Inner Mongolia, China, were collected involving 105 Mongolian medicines, with a total frequency of 284. Among them, there were 18 high-frequency medicines (frequency ≥5), which had a frequency of 147, accounting for 51.76% of the total frequency. The medicine with the highest frequency (14, 4.93%) was Carthamus tinctorius L.. The analysis of the medicine data in China revealed 15 association rules. A total of 21 prescriptions used in Mongolia were collected, involving 92 Mongolian medicines, with a total frequency of 254. Among them, there were 17 high-frequency (frequency ≥5) medicines, with a total frequency of 124 (48.82%). The medicine with the highest frequency (13, 5.12%) was Carthamus tinctorius L.. The analysis of the medicine data in Mongolia revealed 79 association rules. In both regions, the high-frequency medicines used for treating liver disease commonly had cool or warm property with bitter or sweet taste. Conclusions Carthamus tinctorius L., Terminalia chebula Retz., and other Mongolian medicines are commonly used to treat liver diseases in Inner Mongolia and Mongolia. The Mongolian medicines with cool or warm property and bitter, sweet, or astringent taste are commonly used to protect the liver.

Intra and Interspecific Relation of Tree Species Aspects of Seed Biometry, Phenology, Seed Dispersion, and Germination

Bangladesh, with its dense population and diverse ecological landscape, embodies a unique environmental tapestry. This study explores the intricate interplay between tree phenology, seed dispersal seed germination, and biometric characteristics within the context of Bangladesh's rich biodiversity. Through field research conducted from 2020 to 2023 in the Chittagong division, we investigated species such as Artocarpus chama, Avicennia officinalis, Terminalia arjuna, Terminalia bellirica, and Terminalia chebula. The findings reveal significant variations in seed morphology, phenology, germination, and ecological roles among these species. Furthermore, through dendrogram analysis and principal component analysis (PCA), we elucidate the underlying patterns and relationships governing biometric variability. These insights provide valuable contributions to our understanding of ecological processes and inform conservation strategies aimed at preserving Bangladesh's forest tree biodiversity.

Antiviral Activities of Some Traditional Medicinal Plants of Sri Lanka

Abstract: The aim of this article is to review the antiviral activity of plants traditionally used in indigenous medicine in Sri Lanka, their therapeutic potential, chemistry, and botany. Viral infections represent an increasing threat to humans worldwide. Conventional antiviral drugs are available against respiratory viruses. Naturally occurring antiviral activity of medicinal plants was used for centuries in the country’s rich traditional medicine system consisting of Ayurveda, Siddha, Unani, and Deshiya Chikitsa. Traditional physicians cure diseases, including those that originate from viruses, through herbal medicine. To complement this, about 1430 species representing 838 genera, equivalent to 45% of the entire flowering plant community, are considered medicinal. The present article attempts to review the essence of decades of discoveries on antiviral and related properties of 21 medicinal plants, Allium sativum L., Annona muricata L., Ardisia elliptica Thunb., Azadirachta indica A. Juss., Caesalpinia pulcherrima (L.) Sw., Coriandrum sativum L., Coscinium fenestratum (Gaertn.) Colebr., Hedyotis corymbosa (L.) Lam., Hemidesmus indicus (L.) R. Br., Justicia adhatoda L., Ocimum tenuiflorum L., Phyllanthus embilica L., Phyllanthus debilis Klein ex Willd., Piper longum L., Piper nigrum L., Solanum xanthocarpum Schrad & Wendl., Terminalia bellirica (Gaertn.) Roxb., Terminalia chebula Retz., Tinospora cordifolia (Wild) Miers., Vitex negundo L., Zingiber officinale Roscoe. Among the medicinal plants commonly used in Sri Lankan traditional medicine, Justicia adhatoda showed stronger anti-influenza virus activity, inhibiting virus attachment and replication, while Terminalia chebula consisting of chebulagic and chebulinic acids, demonstrated direct antiviral activity against sexually transmitted herpes simplex virus-2 (HSV-2).

Exploring phytochemical diversity and antioxidant potential in leaves extracts: A comparative study of Psidium guajava (Guava) and Terminalia chebula (Myrobalan)

Exploring phytochemical diversity and antioxidant potential in leaves extracts: A comparative study of Psidium guajava (Guava) and Terminalia chebula (Myrobalan)

Phytochemical and Pharmacognostical Evaluation of Kodiveli Churnam - A Polyherbal Siddha Formulation

Kodiveli Churnam is a traditional Siddha formulation made up of Plumbago zeylanica, Pongamia pinnata, Chukrasia tabularis, Piper longum, Brassica juncea, Nigella sativa, Zingiber officinalis and Terminalia chebula. The formulation has been studied using the macroscopic, microscopic, TLC/HPTLC techniques. The pharmacognostical studies revealed the presence of specific characteristic features of the raw drugs. The HPTLC fingerprinting was derived using a specific solvent system which could capture majority of the phytoconstituents of this drug. It is concluded that the formulation of Kodiveli churnam, the HPTLC fingerprinting and microscopic characteristics can be used to validate the preparation and authenticity of the formulation.

Evaluation and comparison of antibacterial efficacy of different concentrations of Chhattisgarh herbal product-Terminalia chebula fruit extract in opposition to Enterococcus faecalis: An invitro study.

Due to the low cost, natural origin, higher safety margins, and little to negligible adverse effects of herbal medications, the use of plants and plant derivatives in medicine is becoming increasingly widespread. Terminalia chebula is among the most significant medicinal plants in ayurvedic, siddha, unani, and homeopathic remedies. It is ranked first in Ayurvedic material medicine. T. chebula has been shown to have established effects against various bacterial and fungal infections, including dental caries pathogens. In recent years, there has been a rise in interest in dentistry and medicine related to Enterococcus faecalis. The research aimed to assess the antibacterial effectiveness of different concentrations of T. chebula ethanolic fruit extract (10%, 40%, and 100%) in opposition to E. faecalis and compare it with 2% chlorhexidine. For the study, T. chebula ethanolic fruit extracts were obtained and prepared with Group I: -10% concentration, Group II: -40% concentration, Group III: -100% concentration, and Group IV: -2% chlorhexidine. Colonies of E. faecalis were cultivated in brain heart infusion (BHI) broth at 37°C and were inoculated in 16 BHI agar plates. Then, on the petri dishes, four wells were created (8 mm diameter) using a metal borer. The Agar well diffusion method was used to examine the antibacterial activity, and the zones of inhibition around the wells were noted. The obtained data were statistically analyzed using one-way ANOVA and post-hoc tests. The result shows that as the concentration increases, there is an increase in the efficacy of the antibacterial property of the extract before it reaches the saturation point. The decreasing order of antibacterial was chlorhexidine >100% T. chebula >40% T. chebula >10% T. chebula. The production of contemporary pharmaceuticals from T. chebula was addressed, as the global scenario is currently evolving toward using nontoxic plant products with traditional medicinal benefits.