Poor treatment adherence, often referred to as the "silent epidemic," is a growing global issue that significantly contributes to preventable illness, premature death, and rising healthcare costs. Among compliance-enhancing strategies, controlled-release depots designed for intermediate treatment cycles represent a suitable approach, particularly in contexts where frequent dosing is impractical. Current long-acting injectable formulations, though, are often hindered by challenges in injectability, delayed onset, and complex manufacturing. Here, we present a thermoresponsive, in situ forming liposomal depot (TILD) designed to modulate the release of membrane-associated drugs following subcutaneous administration. Using buprenorphine as a model analgesic, we demonstrate that TILD responds to subcutaneous divalent cations with immediate surface-drug release and to body temperature with sustained diffusion through a fluidized bilayer. Molecular simulations guided the system design, and structural and colloidal characterizations validated its responsiveness to physiological cues. In vivo, TILD formed stable depots and maintained therapeutic drug levels for up to four days in both rats and Beagle dogs. Pharmacodynamics studies in rats confirmed the delivery of prolonged analgesia.

Navigating the complexities in characterization of NDSRIs using advanced analytical techniques.

Research on the ultrasonic-assisted extraction, purification, structural characterization, and anti- glycolipid metabolic disorder (anti-GLMD) activity and mechanism of polysaccharide from Dryopteridis crassirhizomatis Rhizoma.

Novel amino acid-substituted cycloastragenol derivatives with antibacterial activity: Design, synthesis, structural characterization, and moleculardocking

Synthesis, structural characterization, and biological evaluation of a bioactive strontium (II)-mefenamic acid complex: In-vivo and in-silico insights.

O-methyl-(2,4-dimethoxyphenyl) dithiophosphonate ligand and the Ni(II) complex thereof: Synthesis, structural characterization, X-ray crystallography, theoretical calculations and photophysical properties

Isolation, purification, structural characterization, and In vitro hypoglycemic activity of flaxseed gum modified by lactic acid bacteria

Microbial conversion of banana pseudostem scutcher waste into xylitol using Candida tropicalis (PV544892).

Sesamol-functionalized cyclophosphazenes: Synthesis, structural characterization, and biological evaluation

In this study, 2-(4-methylpiperidin-1-yl)acetohydrazide was condensed with a series of substituted benzaldehydes to afford a library of piperidine-based hydrazones, which were subsequently complexed with K₂PtCl₄ and K₂PdCl₄ to yield the corresponding Pt(II) and Pd(II) complexes. The structures of molecules were elucidated by spectral methods and elemental analysis. The in vitro anticancer potential of the compounds was evaluated through cytotoxicity, wound-healing/migration, and RT-qPCR assays in A549 (lung) and HepG2 (hepatocellular) cancer cell lines. In the cytotoxicity studies, compound 3lPd exhibited the highest activity in A549 cells (IC₅₀ = 61.25 ± 0.03 µM), while ligand 3b and complex 3nPt showed the most pronounced effects in HepG2 cells (IC₅₀ = 77.96 ± 0.05 and 77.01 ± 0.02 µM, respectively). The most active compounds were further tested in wound-healing assays, where 3lPd almost completely inhibited A549 cell migration at 24 and 48 h, whereas the reference drug cisplatin induced only partial inhibition. Consistent with these findings, RT-qPCR analysis revealed that in A549 cells, 3lPd markedly suppressed MYC expression and reduced p53 levels to an extent comparable to cisplatin. In HepG2 cells, 3nPt decreased p53 expression, while L3b uniquely upregulated p53 and maintained MYC expression near control levels. In silico studies were performed to investigate their interactions with p53, GAPDH, and c-MYC proteins. Overall, these results suggest that piperidine-bearing hydrazones and their Pt(II)/Pd(II) complexes represent promising scaffolds for the development of novel anticancer agents.

Structural characterization, DFT-based electronic analysis, Hirshfeld surface study, molecular docking, and in silico drug-likeness evaluation of N-(4-(3-methyl-3mesitylcyclobutyl)-1H-imidazol-2-yl)acetamide

High-pressure homogenized whey protein-milk fat composite salt enhances myofibrillar protein gelation in Litopenaeus vannamei: A mechanistic approach to sodium reduction with improved texture and digestibility.

Structural characterization and antioxidant activity evaluation of a polysaccharide obtained from the wheat aleurone layer fermented by Agrocybe aegerita.

Neuroprotective effects and mechanism of ginseng aqueous extract against Alzheimer's disease.

Synthesis, structural characterization, photophysical properties, and OLED applications of Ir(Ⅲ) complexes

Chiral amino acid–based anthraquinones: synthesis, structural characterization, and structure–activity relationships in cancer cell lines

Synthesis, structural characterization, computational studies, and anticancer evaluation of novel rufloxacin Schiff base metal complexes with involvement of Mcl-1 modulation in breast cancer cells.

Phosphorylation modification enhances the iron-chelating properties of peptides derived from goat blood proteins: Towards a facile one-pot strategy.

Efficient extraction of Cercis chinensis seeds polysaccharides by ultrasound-assisted deep eutectic solvent: Process optimization, identification, kinetics and bioactivity.

Ruthenium-doped metal phosphides with expanded lattices enable efficient alkaline water splitting.