Changes In Root Length Research Articles

Major-effect quantitative trait locus qLKR4.1 encodes a phospholipase Dδ protein associated with low-K+ stress tolerance by promoting root length

qLKR4.1, controlling low K+ resistance in tomato, was fine-mapped to an interval of 67.5kb on chromosome A04, and one gene encoding phospholipase Dδ was identified as a candidate gene. In plants, changes in root length are an important morphological response to low K+ (LK) stress; however, the underlying genetics in tomato remain unclear. Here, we combined bulked segregant analysis-based whole-genome sequencing, single-nucleotide polymorphism haplotyping, and fine genetic mapping to identify a candidate gene as a major-effect quantitative trait loci (QTL), i.e., qLKR4.1, which was associated with LK tolerance due to increased root elongation in the tomato line JZ34. Through multiple analyses, we found that Solyc04g082000 is the most likely candidate for qLKR4.1, which encodes phospholipase Dδ (PLDδ). Increased root elongation under LK in JZ34 may be attributed to a non-synonymous single-nucleotide polymorphism in the Ca2+-binding domain region of this gene. Solyc04g082000 increases root length through its PLDδ activity. Silencing of Solyc04g082000Arg in JZ34 led to a significant decrease in root length compared with silencing of Solyc04g082000His allele in JZ18 under LK conditions. Mutation of a Solyc04g082000 homologue in Arabidopsis, pldδ, resulted in decreased primary root lengths under LK conditions, compared to the wild type. Transgenic tomato expressing the qLKR4.1Arg allele from JZ34 exhibited a significant increase in root length compared with the wild type expressing the allele from JZ18 under LK conditions. Taken together, our results confirm that the PLDδ gene Solyc04g082000 exerts important functions in increasing tomato root length and LK tolerance.

Evaluation of root resorption in the lower incisors after orthodontic treatment of skeletal Class III malocclusion by three-dimensional volumetric measurement with cone-beam computed tomography.

To investigate the volumetric changes of the lower incisor roots in skeletal Class III orthodontic patients with anterior crossbite after premolar extraction therapy. Thirty-six adults, aged 18-28 years, had four-premolar extraction treatment. Pre- and posttreatment cone-beam computed tomography (CBCT) images were used to assess the thickness and height of alveolar bone, root volume, and length. A paired t-test was used to detect changes in root volume and length before and after treatment. Pearson's correlation analysis was applied to estimate the correlation between root volume and dentoskeletal morphology. Both the central and lateral incisors had intrusion and tipping movement after treatment. Compared with pretreatment data, root length decreased significantly. The lingual root volume of root cervical, apical third, and the labial root volume of the root apical third decreased significantly (P < .05), among which the percentage of tooth loss at the tip volume was the highest. The pretreatment height of the alveolar ridge crest, thickness of the alveolar bone, and type of incisor movement were related to the volume and length loss. Volume and length loss in the apical third of the lower incisor roots in skeletal Class III patients treated with a Class III bicuspid extraction pattern is common. The pretreatment height of the alveolar ridge crest, thickness of the alveolar bone, and type of tooth movement are related to the loss.

Efficacy evaluation of some fumigants against Fusarium oxysporum and enhancement of tomato growth as elicitor-induced defense responses

Fusarium wilt, the most serious soil-borne pathogen, is a serious problem for tomato production worldwide. The presented study evaluated the antifungal activity against Fusarium oxysporum f. sp. lycopersici in vitro and in vivo for nine fumigants. In addition, the research examined the possibility of enhancing the growth of tomato plants in order to increase resistance against this disease by using four chemical inducers. The results indicated that at 20 mg/L, the radial growth of the pathogen was inhibited 100% by formaldehyde and > 80% by phosphine. Among the essential oils investigated, neem oil was the most effective, however, it only achieved 40.54% at 500 mg/L. The values of EC50 for all fumigants, except dimethyl disulfide (DMDS) and carbon disulfide (CS2), were lower than those for thiophanate-methyl. Phosphine was the highest efficient. The elicitors can be arranged based on their effectiveness, gibberellic acid (GA3) > sorbic acid > cytokinin (6-benzylaminopurine) > indole-3-butyric acid. The change in root length, fresh weight, and dry weight was greater with soil drench than with foliar application. The fumigant generators formaldehyde, phosphine and 1,4-dichlorobenzene and bio-fumigants citrus and neem oils as well as elicitors gibberellic and sorbic acid could be one of the promising alternatives to methyl bromide against Fusarium oxysporum as an important component of integrated management of Fusarium wilt.

Phytotoxic Effects of Polystyrene and Polymethyl Methacrylate Microplastics on Allium cepa Roots

Plastic contamination has become one of the most pressing environmental issues due to rapidly increasing production of disposable plastic products, their fragmentation into smaller pieces, and long persistence in the environment, which affects all living organisms, including plants. In this study, Allium cepa roots were exposed to 0.01, 0.1, and 1 g L-1 of commercial polystyrene (PS-MPs) and polymethyl methacrylate microparticles (PMMA-MPs) for 72 h. Dynamic light scattering (DLS) analyses showed high stability of both types of MPs in ultrapure water used for A. cepa treatment. Morphometric analysis revealed no significant change in root length compared to control. Pyrolysis hyphenated to gas chromatography and mass spectrometry (Py-GC-MS) has proven PS-MPs uptake by onion roots in all treatments, while PMMA-MPs were recorded only upon exposure to the highest concentration. Neither MPs induced any (cyto)toxic effect on root growth and PMMA-MPs even had a stimulating effect on root growth. ROS production as well as lipid and protein oxidation were somewhat higher in PS-MP treatments compared to the corresponding concentrations of PMMA-MP, while neither of the applied MPs induced significant damage to the DNA molecule assayed with a Comet test. Significantly elevated activity of H2O2 scavenging enzymes, catalase, and peroxidases was measured after exposure to both types of MPs. Obtained results suggest that onion roots take up PS-MPs more readily in comparison to PMMA-MPs, while both types of MPs induce a successful activation of antioxidant machinery in root cells that prevented the occurrence of toxic effects.

The Arabidopsis thaliana trehalose-6-phosphate phosphatase gene AtTPPI regulates primary root growth and lateral root elongation.

Roots are the main organs through which plants absorb water and nutrients. As the key phytohormone involved in root growth, auxin functions in plant environmental responses by modulating auxin synthesis, distribution and polar transport. The Arabidopsis thaliana trehalose-6-phosphate phosphatase gene AtTPPI can improve root architecture, and tppi1 mutants have significantly shortened primary roots. However, the mechanism underlying the short roots of the tppi1 mutant and the upstream signaling pathway and downstream genes regulated by AtTPPI are unclear. Here, we demonstrated that the AtTPPI gene could promote auxin accumulation in AtTPPI-overexpressing plants. By comparing the transcriptomic data of tppi1 and wild-type roots, we found several upregulations of auxin-related genes, including GH3.3, GH3.9 and GH3.12, may play an important role in the AtTPPI gene-mediated auxin transport signaling pathway, ultimately leading to changes in auxin content and primary root length. Moreover, increased AtTPPI expression can regulate primary root growth and lateral root elongation under different concentration of nitrate conditions. Overall, constitutive expression of AtTPPI increased auxin contents and improved lateral root elongation, constituting a new method for improving the nitrogen utilization efficiency of plants.

Quantitative assessment of root resorption in TAD-aided anchorage with and without RAP: A CBCT study on en masse retraction cases.

The purpose of this study was to evaluate the amount of root resorption with mini implant-aided anchorage, with and without inducing the regional acceleratory phenomenon (RAP) in en masse retraction cases using cone-beam computed tomography (CBCT). Thirty patients requiring therapeutic extraction of all first premolars were included in the study and randomly divided into two groups of fifteen patients each (groups I and II). Patients of both groups underwent retraction using mini implants. In patients of group II, the RAP was initiated before starting retraction. For each patient, CBCT of the maxillary and mandibular anterior region was taken before treatment and after retraction to evaluate root length changes. The amount of root resorption post-retraction was lesser in group II as compared to group I for the maxillary canine, mandibular canines, and mandibular lateral incisors. When the amount of root resorption was scored according to the Malmgren index, a greater percentage of teeth in group I (17.8%) showed root resorption of score of 3 as compared to group II (6.1%). The results of the study show that the RAP caused reduced root resorption, but its effect was localized to the area where it was induced. When mini implants are used for absolute anchorage during en masse retraction, the RAP can be induced to reduce the root resorption that results from increased treatment time associated with implant-aided retraction.

Modified pulpotomy procedure in immature permanent teeth with apical periodontitis: a randomised controlled trial

ObjectivesThe objective of this study was to evaluate the effectiveness of a modified pulpotomy (MP) procedure in immature permanent teeth with apical periodontitis (AP).DesignRandomised controlled trial.SettingOne public hospital in Shanghai,...

A RADIOGRAPHIC ANALYSIS OF EXTERNAL APICAL ROOT RESORPTION (EARR) OF MAXILLARY INCISORS AFTER 6 MONTHS OF INITIATION OF FIXED ORTHODONTIC APPLIANCE THERAPY

Consequences of External apical root resorption (EARR) range from slight tooth mobility due to mild amounts of root resorption to complete loss of teeth due to excessive root resorption. The purpose of this study was to measure the amount of EARR that might occur on the 4 maxillary incisor teeth while using the MBT system and to evaluate its clinical signicance during a 6-month period of active treatment. In addition, the contribution of gender, and how extraction and non-extraction of premolars is related to maxillary incisor EARR was examined. A sample of 40 patients between 12 -20 years of both genders reporting to the Department of Orthodontics &amp; Dentofacial Orthopaedics of KD Dental College was selected for this study. The amount of EARR for the 160 teeth was examined. There was no statistically signicance difference (p&gt;0.05) found between change in root length of Maxillary right Central incisor, Maxillary right lateral incisor, Maxillary left central incisor and Maxillary left lateral incisor. There was no statistically signicant difference was found among male and female with EARR and there was no statistical difference (p&gt;0.05) found in external apical root resorption among participants with premolar extraction and without premolar extraction. This study concludes that all the maxillary incisors in this investigation did not show any kind signicant EARR after the follow-up period of 6months of active orthodontic treatment with MBT treatment techniques, no signicant changes in EARR was observed when comparison was made for gender and also for treatment where premolar extraction was done.

Treatment of Palatally Displaced Canines Using Miniscrews for Direct or Indirect Anchorage: A Three-Dimensional Prospective Cohort Study on Tooth Movement Speed

Palatally impacted canines are usually challenging to treat in terms of both biomechanics and possible side effects. Different therapeutic approaches have been reported with or without the use of temporary anchorage devices, including the canine-first approach. Two groups of patients with palatally impacted canines were compared, observing their canine movement measured on consecutive CBCTs after three months of treatment. In the control group, impacted canines were treated with direct anchorage on miniscrews, and in the test group with indirect anchorage using a miniscrew-supported transpalatal arch. The primary outcome was the canine movement speed; the secondary outcome was the change in the root length of adjacent teeth. The median monthly apex speed was 1.05 mm in the control group (IR [0.74, 1.64]) and 0.72 mm in the test group (IR [0.27, 1.30]). The median monthly cusp displacement was 1.89 mm in the control group (IR [1.04, 2.84]) and 1.08 mm in the test group (IR [0.81, 1.91]). Approximately 50% of teeth adjacent to an impacted canine underwent a negative root length change of less than 1 mm in the majority of cases, but no significant differences were found in root length changes between groups. No statistically significant differences in the reported speeds were found, and no miniscrew failures were observed in either group.

Three-dimensional analysis of root changes after orthodontic treatment for patients at different stages of root development

We investigated changes in the roots of maxillary incisors at different stages of root development after fixed-appliance treatment using cone-beam computed tomography. Data from 52 subjects receiving fixed-appliance treatment were collected retrospectively. The subjects were divided into 3 groups: mixed dentition group (aged 7-10 years; root development stage: Nolla eighth-10th; n= 16), early permanent dentition group (aged 12-18 years; root development stage: Nolla 10th; n= 20), and adult group (aged 18-35 years; root development stage: Nolla 10th; n= 16). Changes in root lengths and volume of the maxillary central incisors were measured using pretreatment and posttreatment cone-beam computed tomography. The root lengths and volumes of maxillary central incisors in the mixed dentition group significantly increased after orthodontic treatment (P>0.05). No significant differences were found when comparing the final root length and volume of the mixed dentition group with the pretreatment maxillary incisor values of the early permanent dentition group (P>0.05). The early permanent dentition group showed a significant decrease in root length (P<0.05), and both the root length and volume of the adult group significantly decreased after treatment (P<0.05). The differences in root length and volume reduction between the 2 groups were not significant (P>0.05). Orthodontic treatment had no significant negative impact on the continued root development of incomplete roots with two-thirds root formation. Both the early permanent dentition and adult groups exhibited root resorption after orthodontic treatment. It seemed age was not a factor that resulted in significant root resorption during routine orthodontic leveling and alignment treatment once the roots were fully developed.

Mixed cattle and sheep grazing reduces the root lifespan of the community in a desert steppe

• Mixed cattle and sheep grazing can significantly shorten root longevity and may accelerate carbon sequestration. • Grazing inhibits fine root longevity of plant community in the temperate desert steppe. • Sheep grazing alone has a greater inhibitory effect on root longevity than cattle grazing alone. • Adjusting the combination of herbivores is an effective strategy to shorten the root lifespan of the community. • The factors affecting root longevity were analyzed by the COX proportional-hazards model. The soil is the primary carbon pool in grassland ecosystems, and the turnover of plant roots is an essential way of organic carbon input. Grazing intensity under a single animal species is usually used to explore the effects of herbivores on grassland, but multi-species grazing is common in grassland utilization. However, few studies have examined the impact of different livestock species and herbivore combinations on the root growth of the grassland community. We used the minirhizotron method to explore the response of root growth in a temperate desert steppe community to cattle and sheep grazing over two years. Among root characteristics and environmental factors, grazing method(cattle grazing; sheep grazing; cattle and sheep grazing; no grazing) is the most significant inhibiting factor affecting root longevity. We found that mixed cattle and sheep grazing significantly reduced the root lifespan of the community, with a median survival time of 120 d, and a survival rate of only 30 % at the end of the observation period. Compared with other treatments, mixed cattle and sheep grazing significantly reduced the number of live roots (0.3724 ± 0.0453 piece/cm 2 ). The change in fine root length of mixed grazing (-0.012 ± 0.0013 mm/cm 2 ) was considerably greater than other treatments. The root lifespan of the community was significantly negatively correlated with the ratio of belowground to aboveground net primary productivity (BNPP/ANPP). The mixed cattle and sheep grazing changed in the patterns of plant carbon contribution, and increased the organic carbon content of the soil. Therefore, under moderate grazing intensity, adjusting the combination of herbivores is an effective strategy to accelerate root turnover rate of the community, accelerate the input of carbon from plant photosynthesis into the soil carbon pool, and may increase soil carbon storage.

Understanding the Role of Physiological and Agronomical Traits during Drought Recovery as a Determinant of Differential Drought Stress Tolerance in Barley

The fast and efficient recovery could be an important trait defining the efficacy of plant drought adaptation. In this work, we aimed to develop a set of simple and appropriate physiological proxies that could be used as reliable indicators to predict plant drought responses and validate the role of specific physiological traits such as root length, stomata density, and residual transpiration, in the drought tolerance and recovery in barley. Eighty barley (Hordeum vulgare L.) genotypes were subjected to progressive droughting until the soil moisture level reached 10%, followed by rewatering. Plants were visually scored at the end of drought period and two weeks after rewatering. SPAD values and chlorophyll fluorescence Fv/Fm ratio were also measured, alongside with stomatal density (SD) and residual transpiration (RT). The same genotypes were germinated in paper rolls treated with 15% (w/v) of polyethylene glycol (PEG) 8000 by quantification of changes in the root growth patterns. Responses to drought stress varied among the genotypes, and drought tolerance and recovery scores were significantly correlated with each other. Changes in SPAD value, Fv/Fm ratio and root length were significantly correlated with the drought tolerance and recovery indices. Both indices correlated strongly with the SD and RT of irrigated plants, although in an unexpected direction. We have also correlated the extent of plants’ drought tolerance to their ability to grow in saline soils (a condition often termed a “physiological drought”) and found a positive association between these two traits. The fact that drought tolerant genotype also possessed higher salinity tolerance implies some common mechanisms conferring both traits. Plants having less SD and more RT under irrigated conditions showed higher drought tolerance. It is concluded that lower SD and higher RT under optimal conditions may be used as proxies for drought tolerance in barley.

Local nitrogen application increases maize post-silking nitrogen uptake of responsive genotypes via enhanced deep root growth

Nitrogen (N) is unevenly distributed throughout the soil and plant roots proliferate in N-rich soil patches. However, the relationship between the root response to localized N supply and maize N uptake efficiency among different genotypes is unclear. In this study, four maize varieties were evaluated to explore genotypic differences in the root response to local N application in relation to N uptake. A split-root system was established for hydroponically-grown plants and two methods of local N application (local banding and local dotting) were examined in the field. Genotypic differences in the root length response to N were highly correlated between the hydroponic and field conditions (r>0.99). Genotypes showing high response to N, ZD958, XY335 and XF32D22, showed 50–63% longer lateral root length and 36–53% greater root biomass in N-rich regions under hydroponic conditions, while the LY13 genotype did not respond to N. Under field conditions, the root length of the high-response genotypes was found to increase by 66–75% at 40–60 cm soil depth, while LY13 showed smaller changes in root length. In addition, local N application increased N uptake at the post-silking stage by 16–88% in the high-response genotypes and increased the grain yield of ZD958 by 10–12%. Moreover, yield was positively correlated with root length at 40–60 cm soil depth (r=0.39). We conclude that local fertilization should be used for high-response genotypes, which can be rapidly identified at the seedling stage, and selection for “local-N responsive roots” can be a promising trait in maize breeding for high nitrogen uptake efficiency.

Leading nutrient foraging strategies shaping by root system characteristics along the elevations in rubber (Hevea brasiliensis) plantations.

When it comes to root and mycorrhizal associations that define resource acquisition strategy, there is a need to identify the leading dimension across root physiology, morphology, architecture and whole plant biomass allocation to better predict the plant's responses to multiple environmental constraints. Here, we developed a new framework for understanding the variation in roots and symbiotic fungi by quantifying multiple-scale characteristics, ranging from anatomy to the whole plant. We chose the rubber (Hevea brasiliensis) grown at three elevations to test our framework and to identify the key dimensions for resource acquisition. Results showed that the quantities of absorptive roots and root system architecture, rather than single root traits, played the leading role in belowground resource acquisition. As the elevation increased from the low to high elevation, root length growth, productivity and root mass fraction (RMF) increased by 2.9-, 2.3- and 13.8-fold, respectively. The contribution of RMF to the changes in total root length was 3.6-fold that of specific root length (SRL). Root architecture exhibited higher plasticity than anatomy and morphology. Further, mycorrhizal colonization was highly sensitive to rising elevations with a non-monotonic pattern. By contrast, both leaf biomass and specific leaf area (traits) co-varied with increasing elevation. In summary, rubber trees changed root system architecture by allocating more biomass and lowering the reliance on mycorrhizal fungi rather than improving single root efficiency in adapting to high elevation. Our framework is instructive for traits-based ecology; accurate assessments of forest carbon cycling in response to resource gradient should account for the leading dimension of root system architecture.

Root Length Changes in Orthodontically Displaced Teeth Treated with the Corticotomy Approach

The aim of the study: Corticotomy-facilitated orthodontics is a modern approach to resolve complicated orthodontic cases that may increase the pace of tooth movement. The study's goal was to assess the changes that occurred at the root level following orthodontic treatment when corticotomy was used. Material and methods: Based on Cone Beam Computer Tomography, measurements of the root length at T0 (before corticotomy) and T1 (after corticotomy) were taken after splitting the individuals into two groups (maxillary and mandibular corticotomy) (6 months after surgery). For statistical analysis of the data, many tests were utilized. Results: The root length values obtained at T1 showed minimal changes in length, with statistically insignificant values (for the maxillary arch, the values obtained were 13.36 ± 2.41 mm for women and 14.26 ± 2.06 mm for men; for the lower arch, the measured values were 12.38 ± 2.09 mm for women and 11.56 ± 2.29 mm for men). The canine on the left hemiarcade had the most significant change in root length following treatment, with a value assessed at T1 of 16.72 ± 1.78 mm, which was statistically significant, p 0.05. Conclusion: According to the data obtained in this study, when orthodontic therapy is associated with corticotomy, there is a decrease in root resorption that may occur in the case of conventional orthodontic treatments.

Rewetting prolongs root growing season in minerotrophic peatlands and mitigates negative drought effects

Abstract Root phenology influences the timing of plant resource acquisition and carbon fluxes into the soil. This is particularly important in fen peatlands, in which peat is primarily formed by roots and rhizomes of vascular plants. However, most fens in Central Europe are drained for agriculture, leading to large carbon losses, and further threatened by increasing frequency and intensity of droughts. Rewetting fens aims to restore the original carbon sink, but how root phenology is affected by drainage and rewetting is largely unknown. We monitored root phenology with minirhizotrons in drained and rewetted fens (alder forest, percolation fen and coastal fen) as well as its soil temperature and water table depth during the 2018 drought. For each fen type, we studied a drained site and a site that was rewetted ~25 years ago, while all the sites studied had been drained for almost a century. Overall, the growing season was longer with rewetting, allowing roots to grow over a longer period in the year and have a higher root production than under drainage. With increasing depth, the growing season shifted to later in time but remained a similar length, and the relative importance of soil temperature for root length changes increased with soil depth. Synthesis and applications. Rewetting extended the growing season of roots, highlighting the importance of phenology in explaining root productivity in peatlands. A longer growing season allows a longer period of carbon sequestration in form of root biomass and promotes the peatlands' carbon sink function, especially through longer growth in deep soil layers. Thus, management practices that focus on rewetting peatland ecosystems are necessary to maintain their function as carbon sinks, particularly under drought conditions, and are a top priority to reduce carbon emissions and address climate change.

OsSPL14 is involved in nitrogen-deficiency-induced root elongation in rice

Nitrogen (N) is an essential element for rice (Oryza sativa L.) growth. Rice responds to N-limiting conditions by changing root morphology. Strigolactones (SLs) and auxin modulate rice root elongation under N-deficient conditions. However, the interactions between SLs and auxin in the regulation of root elongation in response to N deficiency remain needing further study. Here, we analysed the function of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 14 (SPL14, a downstream transcription factor of strigolactone signaling) in regulating root elongation under low N (LN) supply. Compared with sufficient N (SN) condition, LN and SN+GR24 (SL analogue) reduced protein interaction between SLs signaling repressor DWARF 53 (D53) and SPL14. The expression of SPL14 was induced by LN supply. In contrasts to WT plants, knockdown of SPL14 resulted in less extensive changes in root length under LN condition. Overexpression of SPL14 under SN supply enhanced root length to the same extent as WT plants under LN condition, suggesting that SPL14 was induced by LN condition and led to changes in root elongation. The transcription of PIN-FORMED 2 and 10b (auxin efflux carriers) were negatively and positively regulated by SPL14, respectively. Under SN and LN conditions, similar root morphology was observed in overexpressing PIN10b and SPL14 lines, and opposite root phenotypes were recorded between pin2 mutants and SPL14-RNAi lines.These results indicated that PIN2 and PIN10b were involved in SPL14-induced root elongation under LN condition. Therefore, we presented that, LN inhibits D53 and SPL14 interaction, which releases SPL14 to modulate the transcription of PIN2 and PIN10b, resulting in root elongation in rice.

Study of vital inflamed pulp therapy in immature permanent teeth with irreversible pulpitis and apical periodontitis

To assess the treatment effectiveness of vital inflamed pulp therapy (VIPT) in immature permanent teeth with irreversible pulpitis and apical periodontitis. The faculty members in the Department of Pediatric Dentistry, the Ninth People's Hospital were invited to submit consecutive VIPT cases from June 2015 to June 2016 (follow-up periods>12 months). The cases were retrospectively reviewed, clinical symptoms and radiographic changes in periapical radiolucency were evaluated, meanwhile, the data of radiographic changes such as apical diameter and root length were calculated and analyzed with ANOVA. Totally thirteen submitted patients/cases were included (6 males and 7 females) in the present study,. The average age of patients was (9.9±1.4) years old. The average follow-up time was (26.5±6.8) months (17-37 months). At the 12-month visit, all 13 treated teeth survived, 9 out of 11 teeth with apical periodontitis showed normal radiographic manifestation. At the 3, 6 and 12 months visits, the within-case percentage changes in apical diameter were (8.0±5.1)%, (24.1±9.1)% and (70.3±10.7)%, respectively, while the within-case percentage changes in root length were (11.4±9.8)%, (14.5±9.8)% and (27.4±14.2)%, respectively. There were statistically significant differences in the changes of apical diameter (F=18.80, P<0.001) and root length (F=4.64, P=0.047) from the preoperative time to the postoperative follow-ups. VIPT might improve clinical outcomes, even achieve continued root development. VIPT can be an option in treating immature teeth with irreversible pulpitis and apical periodontitis.

Short-duration exposure of 3-µm polystyrene microplastics affected morphology and physiology of watermilfoil (sp. roraima)

Microplastics are one of the most widely discussed environmental issues worldwide. Several studies have shown the effect of microplastic exposure on the marine environment; however, studies on freshwater systems are lacking. This study was conducted to investigate the effect of microplastics on hydroponically growing emergent freshwater macrophytes, watermilfoil (sp. roraima) under controlled environmental conditions. Plants were exposed to 0mg L-1 (control), 0.05mg L-1, 0.25mg L-1, 1.25mg L-1, and 6mg L-1 of 3-µm polystyrene microspheres for 7days. The oxidative stress, antioxidant response, pigmentations, Fv/Fm, and growth parameters in the above-water and below-water parts were analyzed separately. Microscopic observations were performed to confirm the tissue absorbance of the microplastics. Exposure to microplastics altered some parameters; however, growth was not affected. The effect of microplastics was not linear with the exposure concentration for most of the parameters and between 1.25 and 6mg L-1 concentrations. The response trends mostly followed the second-order polynomial distributions. Under the 1.25mg L-1 exposure, there were significant changes in root length, H2O2 content, catalase activity, anthocyanin content, and Fv/Fm. There were differences in parameters between the above-water and below-water parts, and the responses of the microplastics followed different trends. Microscopic observations confirmed the attachment of microplastic particles onto newly formed roots, except for older roots or shoot tissues.

Evaluating Slow Pyrolysis of Parthenium hysterophorus Biochar: Perspectives to Acidic Soil Amelioration and Growth of Selected Wheat (Triticum aestivum) Varieties.

Application of biochar on acidic soils may improve soil fertility and crop productivity. This study aimed to explore the relevance of parthenium biochar-induced changes in the physicochemical properties and agronomic performance of the selected wheat varieties in acidic soils. A pot trial was used in determining the effect of slow pyrolysis parthenium biochar on acidic soils and the agronomic performance of wheat varieties. A general linear model (GLM) of multivariate analysis and principal component analysis (PCA) was used to compare functional variation among soil assayed parameters with biochar dosages and years. Biochar-treated acidic soils did not show significant differences in their physical properties. However, a significant incremental trend was observed in the soil moisture content. The biochar-amended acidic soils showed noticeable differences in the soil pH, available phosphorous, and exchangeable bases (Ca, K, and Na) compared to the control. In all soil samples, a decreasing trend in the soil micronutrients was observed with an increase in the biochar amounts. The analysis also unveiled significant changes in root length, root and shoot dry biomass, and plant height of wheat varieties in response to the biochar amendments. The application of 19.5 t/ha and 23 t/ha dosages of biochar gave the maximum changes in the agronomic performance of Kekeba and Ogolcha varieties, while the minimum was obtained in the 26.5 t/ha and the control. Furthermore, PCA axis 1 accounted for 74.34% of the total variance within a higher eigenvector value (10.4076), and most of the soil parameters were positively correlated with CEC (0.29), available phosphorous (0.29), and soil pH (0.28); however, the micronutrients were negatively correlated. In conclusion, Parthenium hysterophorus biochar has the potential to amend acidic soils, and thus, the application of 16.0, 19.5, and 23 t·ha−1 biochar dosages are considered suitable to reduce the soil acidity level and improve the agronomic performance of wheat varieties. However, extensive research will be needed to determine the effects of biochar on soil properties and crop production in field conditions.